Rw4G036890

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0007229
Physical Location & Seq
Reverse (-)
63692242 .. 63702182
9941 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G036890.1

Sequence Viewer

Length: 2349 bp
ATGGCAGCATTTATCAAGCTCGAGGACTCCCCAATGTTCCAGAAGCAGGTATGTAATCTAGAACAGACAGCTGATGAGTTGAAAGAGCGATGCCAAAAACTATTCAAAGGGTGTAAGAAGTTTATGGCGGCTCTTGGAGAAGCATGTAATGGCGACACCACTTTTGCAGATTCATTGGAAGCATTTGGTGGTGATCTGGATGATCCTATAAGTGTTGCAATAGGAGGTCCAGTACTATCCAAATTCATTTCTGCATTTCGAGAGCTTGCTACATATAAAGAGCTTCTTCGCACCCAAGTAGAGCATGTGTTAGTCAATCGGTTGATGCACTTTATGTCTGTTGATCTGCAAGATGCAAAGGAGTCCCGACGCCGCTTTGACAAGGCAATACATGTTTATGATCAGTCACGGGAAAAGTTTGTATCCTTAAAGAAGAATACACGGGGAGACATTGTTTCTGAACTAGAGGAGTGTGATCTACAAAACTCAAAATCAGCATTCGAGAAAGGCCGCTTTAACCTAGTAAATTCCCTAATGAATATTGAAGCAAAAAAGAAGTATGAATTTTTGGAATCTATTAGTGCAATCATGGATGGTCATCTAAGATACTTTAAGCTGGGATATGAGTTATTGAGCCAAATGGAGCCATATATTCACCAGGTGTTAACATATGCACAACAATCTAAAGAACAGGCTACTGTTGAACAAGATAAACTTCACAAAAGGATTCAGGAATTTAGGACACAAGCTGAGATGGACAGTTTACGAGCTTCTAGTAACTTTGAGGCTTCTGCAGGGGTTGAAGGACATCGTGCCTTTGGTTTGGCTTCATACAAAAACACAGAACCAATCACCTTTGAAAATGGGAAGCCACAGACAATCAAGGAAGGATATCTTCTGAAACGATCGTCAAATTTGAGGGGAGACTGGAAGAGAAGGTTTTTCGTACTGAACAGTCAAGGGGCTTTATTTTATTACAGAATCAAGGGTACCAAACCCATGGGATCTCAAGCACATCACTTTACACGTTCAACTGAACATAATAGCGGTGTTTTTGGGAGATTCCGCTCAAAAAATAGGGCATCGTCACTTAATGAAAACATTCTGGGCTGCCGAACTGTTGATCTGTGCACTTCAACAATTAAGATGGATGCAGAAGATACAGACCTTCGACTTTGCTTCAGAATAATATCTCCATCGAAAACGTACACGTTGCAGGCTGAAAATGAGGCCGATAGGATGGATTGGATGAACAAAATAACAGGAGCCATTACATCTCTGCTGAATTTTCGGCTTCTTGAACAGCCACATCCGGGAAATGCGAATCTCGAGGGTAGTGAATTAGGTGTTGGTGATGTTCGTCGAGTAGCTAGCTGTGAAAGCTTACATAACATACACTTCAATAGAGTTGATTCCGTTTCTACTGCTCTTAGGGGAATCCCTGGAAATGATCTTTGTGCAGAGTGCAGTAGTCCTGAGCCGGATTGGGCTTCTCTCAATCTTGGGATACTGTTATGCATTGAATGTTCTGGTGCTCATCGGAATCTTGGTGTACATATTTCAAAGGTGAGATCTATAACATTAGATGTCAAAGTTTGGGAACCGACAATTGTGGACTTATTTCGCAACTTGGGTAATGCCTACTGTAATTCTGTGTGGGAGGGAAAGCTTCAACTCAAAAACGAAAGATTGGAGGGCTCAAATGCCATTAGGGCTTCAATTTCAAAACCATGTCCCCAAGATGCAATTCAGCATAAGGAAATTTACATTCAAGCAAAGTATGTAGAGAAAGTGATGGTCATTAGAGATGTAGCTGTGCCTGTCCTTGCAGCAAGTATATGGGAAGCAGTCAAGACTAGTAACTTACAAGAAGTCTATCGGCTTATTGTGATATCAGATATGAATATCATCGATACCACCTATGATGATGTGGTTGGCGATGATTTATATCACCATGTTGATGCACAAGACTCAGAGTTGAGTTTTCCCTTAATAGAGAGCAAACAGCACGATCCAGCAGCATGTGAGAGAATCAAGACTTCCAATGATCAAGGGAACTGCCTTCAAGGATGTTCATTATTGCATCTGGCTTGTCACTCTGGCAATGCAATGATGCTCGAATTGTTGCTGCAATTTGGTGCCAACATAAATAGGCGTGATTTCCATGGAAGGACTCCTTTACACCACTGTATCTGCAGTGGGAATAATTCGCTGGCGAAGTTCCTACTTAGAAGAGGTGCACGCCCATCAATTCAAGATGGAGGAGGGCAAAGCGTACTGGAAAGAGCAATGGAGATGGGAGCGATTACGGATGAGGATCTATTCATAAAACTTTCTGAACATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_5 ArfGap BAR_3 PH

Protein Analysis

782

Amino Acids

88.03

Weight (kDa)

6.36

Isoelectric Point (pI)

45.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BAR_3 PF16746 7 - 239 8.6e-36 BAR domain of APPL family
BAR PF03114 18 - 218 1.1e-11 BAR domain
PH PF00169 294 - 424 2.2e-13 PH domain
ArfGap PF01412 474 - 599 9e-31 Putative GTPase activating protein for Arf
Ank_2 PF12796 676 - 753 6e-13 Ankyrin repeats (3 copies)
Ank_5 PF13857 686 - 729 4.1e-06 Ankyrin repeats (many copies)
Ank PF00023 690 - 720 1.7e-06 Ankyrin repeat
Ank_4 PF13637 694 - 743 3.4e-07 Ankyrin repeats (many copies)
Ank PF00023 722 - 753 8.2e-06 Ankyrin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 37
Acc65I GGTACC 1 cut(s) 989
AccB1I GGYRCC 2 cut(s) 989, 2138
AccBSI CCGCTC 1 cut(s) 1068
AciI CCGC 5 cut(s) 128, 373, 511, 1047, 1066
AclWI GGATC 4 cut(s) 197, 1012, 2006, 2325
AcsI RAATTY 7 cut(s) 242, 526, 563, 734, 913, 1285, 1761
AcuI CTGAAG 1 cut(s) 1165
AcyI GRCGYC 1 cut(s) 370
AdeI CACNNNGTG 1 cut(s) 661
AfaI GTAC 6 cut(s) 234, 948, 991, 1208, 1554, 2277
AfiI CCNNNNNNNGG 2 cut(s) 46, 1313
AflIII ACRYGT 3 cut(s) 391, 1025, 1209
AhlI ACTAGT 1 cut(s) 1856
AjnI CCWGG 2 cut(s) 657, 1441
AjuI GAANNNNNNNTTGG 4 cut(s) 1332, 1364, 1731, 1763
Alw21I GWGCWC 3 cut(s) 1133, 1537, 2242
Alw26I GTCTC 2 cut(s) 441, 918
Alw44I GTGCAC 2 cut(s) 1129, 2238
AlwI GGATC 4 cut(s) 197, 1012, 2006, 2325
Ama87I CYCGRG 2 cut(s) 20, 1328
AoxI GGCC 2 cut(s) 508, 1230
ApaLI GTGCAC 2 cut(s) 1129, 2238
ApeKI GCWGC 5 cut(s) 5, 1110, 1829, 2018, 2128
ApoI RAATTY 7 cut(s) 242, 526, 563, 734, 913, 1285, 1761
ArsI GACNNNNNNTTYG 2 cut(s) 146, 178
Asp700I GAANNNNTTC 1 cut(s) 1101
Asp718I GGTACC 1 cut(s) 989
AspS9I GGNCC 1 cut(s) 227
AsuC2I CCSGG 1 cut(s) 1314
AsuHPI GGTGA 6 cut(s) 203, 647, 844, 1364, 1579, 1943
AsuNHI GCTAGC 1 cut(s) 1370
AvaI CYCGRG 2 cut(s) 20, 1328
AvaII GGWCC 1 cut(s) 227
BaeGI GKGCMC 2 cut(s) 1133, 2242
BaeI ACNNNNGTAYC 2 cut(s) 2173, 2206
BanI GGYRCC 2 cut(s) 989, 2138
BanII GRGCYC 1 cut(s) 1700
Bbv12I GWGCWC 3 cut(s) 1133, 1537, 2242
BbvI GCAGC 5 cut(s) 17, 1097, 1841, 2030, 2115
BccI CCATC 9 cut(s) 587, 748, 1141, 1204, 1234, 1789, 2252, 2254, 2290
BciT130I CCWGG 2 cut(s) 659, 1443
BciVI GTATCC 2 cut(s) 433, 1500
BclI TGATCA 2 cut(s) 400, 2047
BcnI CCSGG 1 cut(s) 1314
BcoDI GTCTC 2 cut(s) 441, 918
BcuI ACTAGT 1 cut(s) 1856
BfaI CTAG 6 cut(s) 59, 464, 521, 774, 1371, 1857
BfmI CTRYAG 2 cut(s) 792, 2194
BfuAI ACCTGC 1 cut(s) 37
BfuI GTATCC 2 cut(s) 433, 1500
BglI GCCNNNNNGGC 1 cut(s) 1712
BglII AGATCT 1 cut(s) 1571
BisI GCNGC 8 cut(s) 6, 129, 373, 511, 1111, 1830, 2019, 2129
BlsI GCNGC 8 cut(s) 7, 130, 374, 512, 1112, 1831, 2020, 2130
BmcAI AGTACT 1 cut(s) 234
Bme1390I CCNGG 3 cut(s) 659, 1314, 1443
Bme18I GGWCC 1 cut(s) 227
BmeT110I CYCGRG 2 cut(s) 20, 1328
BmgT120I GGNCC 1 cut(s) 227
BmiI GGNNCC 5 cut(s) 645, 991, 1267, 1602, 2140
BmrFI CCNGG 3 cut(s) 659, 1314, 1443
BmsI GCATC 9 cut(s) 80, 315, 343, 1091, 1141, 1732, 1951, 2092, 2103
BmtI GCTAGC 1 cut(s) 1374
Bpu10I CCTNAGC 1 cut(s) 1476
BpuEI CTTGAG 1 cut(s) 993
BpuMI CCSGG 1 cut(s) 1314
Bsa29I ATCGAT 1 cut(s) 1911
BsaBI GATNNNNATC 3 cut(s) 597, 1947, 2316
BsaHI GRCGYC 1 cut(s) 370
BsaJI CCNNGG 3 cut(s) 999, 1441, 2164
BsaXI ACNNNNNCTCC 4 cut(s) 216, 246, 438, 468
Bsc4I CCNNNNNNNGG 2 cut(s) 46, 1313
Bse1I ACTGG 3 cut(s) 230, 932, 2283
Bse3DI GCAATG 3 cut(s) 2110, 2115, 2295
Bse8I GATNNNNATC 3 cut(s) 597, 1947, 2316
BseBI CCWGG 2 cut(s) 659, 1443
BseCI ATCGAT 1 cut(s) 1911
BseDI CCNNGG 3 cut(s) 999, 1441, 2164
BseGI GGATG 8 cut(s) 205, 598, 1156, 1245, 1254, 1309, 2075, 2317
BseJI GATNNNNATC 3 cut(s) 597, 1947, 2316
BseLI CCNNNNNNNGG 2 cut(s) 46, 1313
BseMI GCAATG 3 cut(s) 2110, 2115, 2295
BseMII CTCAG 3 cut(s) 741, 1467, 1986
BseNI ACTGG 3 cut(s) 230, 932, 2283
BseRI GAGGAG 2 cut(s) 482, 2277
BseSI GKGCMC 2 cut(s) 1133, 2242
BseXI GCAGC 5 cut(s) 17, 1097, 1841, 2030, 2115
BseYI CCCAGC 1 cut(s) 616
BsgI GTGCAG 2 cut(s) 1479, 1486
Bsh1285I CGRYCG 1 cut(s) 908
BshFI GGCC 2 cut(s) 510, 1232
BshNI GGYRCC 2 cut(s) 989, 2138
BshVI ATCGAT 1 cut(s) 1911
BsiEI CGRYCG 1 cut(s) 908
BsiHKAI GWGCWC 3 cut(s) 1133, 1537, 2242
BsiHKCI CYCGRG 2 cut(s) 20, 1328
BsiSI CCGG 2 cut(s) 1313, 1481
BslFI GGGAC 2 cut(s) 349, 1719
BslI CCNNNNNNNGG 2 cut(s) 46, 1313
BsmAI GTCTC 2 cut(s) 441, 918
BsmFI GGGAC 2 cut(s) 349, 1719
BsmI GAATGC 1 cut(s) 497
BsnI GGCC 2 cut(s) 510, 1232
BsoBI CYCGRG 2 cut(s) 20, 1328
Bsp1286I GDGCHC 4 cut(s) 1133, 1537, 1700, 2242
Bsp1407I TGTACA 1 cut(s) 1552
Bsp19I CCATGG 2 cut(s) 999, 2164
BspACI CCGC 5 cut(s) 128, 373, 511, 1047, 1066
BspANI GGCC 2 cut(s) 510, 1232
BspCNI CTCAG 3 cut(s) 742, 1468, 1985
BspDI ATCGAT 1 cut(s) 1911
BspLI GGNNCC 5 cut(s) 645, 991, 1267, 1602, 2140
BspMAI CTGCAG 2 cut(s) 796, 2198
BspMI ACCTGC 1 cut(s) 37
BspOI GCTAGC 1 cut(s) 1374
BspPI GGATC 4 cut(s) 197, 1012, 2006, 2325
BspT107I GGYRCC 2 cut(s) 989, 2138
BsrBI CCGCTC 1 cut(s) 1068
BsrDI GCAATG 3 cut(s) 2110, 2115, 2295
BsrGI TGTACA 1 cut(s) 1552
BsrI ACTGG 3 cut(s) 230, 932, 2283
BssECI CCNNGG 3 cut(s) 999, 1441, 2164
BssNI GRCGYC 1 cut(s) 370
BssT1I CCWWGG 2 cut(s) 999, 2164
Bst2UI CCWGG 2 cut(s) 659, 1443
Bst4CI ACNGT 7 cut(s) 700, 761, 956, 1120, 1512, 1646, 2189
Bst6I CTCTTC 2 cut(s) 926, 2227
BstACI GRCGYC 1 cut(s) 370
BstAUI TGTACA 1 cut(s) 1552
BstC8I GCNNGC 5 cut(s) 267, 1218, 1372, 2214, 2242
BstDEI CTNAG 6 cut(s) 602, 750, 1430, 1476, 1972, 2228
BstDSI CCRYGG 2 cut(s) 999, 2164
BstF5I GGATG 8 cut(s) 205, 598, 1156, 1245, 1254, 1309, 2075, 2317
BstMAI GTCTC 2 cut(s) 441, 918
BstMCI CGRYCG 1 cut(s) 908
BstMWI GCNNNNNNNGC 2 cut(s) 1380, 1712
BstNI CCWGG 2 cut(s) 659, 1443
BstNSI RCATGY 4 cut(s) 147, 308, 395, 2025
BstSCI CCNGG 3 cut(s) 657, 1312, 1441
BstSFI CTRYAG 2 cut(s) 792, 2194
BstSLI GKGCMC 2 cut(s) 1133, 2242
BstV1I GCAGC 5 cut(s) 17, 1097, 1841, 2030, 2115
BstX2I RGATCY 3 cut(s) 1004, 1571, 2317
BstXI CCANNNNNNTGG 1 cut(s) 1000
BstYI RGATCY 3 cut(s) 1004, 1571, 2317
Bsu15I ATCGAT 1 cut(s) 1911
BsuI GTATCC 2 cut(s) 433, 1500
BsuRI GGCC 2 cut(s) 510, 1232
BsuTUI ATCGAT 1 cut(s) 1911
BtgI CCRYGG 2 cut(s) 999, 2164
BtgZI GCGATG 2 cut(s) 103, 1953
BtsCI GGATG 8 cut(s) 205, 598, 1156, 1245, 1254, 1309, 2075, 2317
BtsI GCAGTG 1 cut(s) 2203
BtsIMutI CAGTG 2 cut(s) 2185, 2203
BveI ACCTGC 1 cut(s) 37
Cac8I GCNNGC 5 cut(s) 267, 1218, 1372, 2214, 2242
Cfr13I GGNCC 1 cut(s) 227
ClaI ATCGAT 1 cut(s) 1911
CseI GACGC 1 cut(s) 378
CsiI ACCWGGT 1 cut(s) 657
Csp6I GTAC 6 cut(s) 233, 947, 990, 1207, 1553, 2276
CviQI GTAC 6 cut(s) 233, 947, 990, 1207, 1553, 2276
DdeI CTNAG 6 cut(s) 602, 750, 1430, 1476, 1972, 2228
DraIII CACNNNGTG 1 cut(s) 661
Eam1104I CTCTTC 2 cut(s) 926, 2227
EarI CTCTTC 2 cut(s) 926, 2227
Eco130I CCWWGG 2 cut(s) 999, 2164
Eco24I GRGCYC 1 cut(s) 1700
Eco32I GATATC 2 cut(s) 893, 1893
Eco47I GGWCC 1 cut(s) 227
Eco57I CTGAAG 1 cut(s) 1165
Eco88I CYCGRG 2 cut(s) 20, 1328
EcoRII CCWGG 2 cut(s) 657, 1441
EcoRV GATATC 2 cut(s) 893, 1893
EcoT14I CCWWGG 2 cut(s) 999, 2164
EcoT22I ATGCAT 1 cut(s) 1520
EcoT38I GRGCYC 1 cut(s) 1700
ErhI CCWWGG 2 cut(s) 999, 2164
FalI AAGNNNNNCTT 8 cut(s) 270, 302, 699, 731, 879, 911, 2161, 2193
FaqI GGGAC 2 cut(s) 349, 1719
FauNDI CATATG 1 cut(s) 670
FbaI TGATCA 2 cut(s) 400, 2047
Fnu4HI GCNGC 8 cut(s) 6, 129, 373, 511, 1111, 1830, 2019, 2129
FokI GGATG 8 cut(s) 212, 605, 1163, 1252, 1261, 1296, 2082, 2324
FriOI GRGCYC 1 cut(s) 1700
Fsp4HI GCNGC 8 cut(s) 6, 129, 373, 511, 1111, 1830, 2019, 2129
FspBI CTAG 6 cut(s) 59, 464, 521, 774, 1371, 1857
GluI GCNGC 8 cut(s) 6, 129, 373, 511, 1111, 1830, 2019, 2129
GsaI CCCAGC 1 cut(s) 620
HaeIII GGCC 2 cut(s) 510, 1232
HapII CCGG 2 cut(s) 1313, 1481
HgaI GACGC 1 cut(s) 378
Hin1I GRCGYC 1 cut(s) 370
HincII GTYRAC 1 cut(s) 666
HindII GTYRAC 1 cut(s) 666
HindIII AAGCTT 2 cut(s) 1381, 1667
HpaI GTTAAC 1 cut(s) 666
HpaII CCGG 2 cut(s) 1313, 1481
HphI GGTGA 6 cut(s) 203, 647, 844, 1364, 1579, 1943
Hpy166II GTNNAC 7 cut(s) 666, 764, 1131, 1209, 1553, 1615, 2240
Hpy188I TCNGA 7 cut(s) 460, 900, 1184, 1542, 1897, 1975, 2338
Hpy8I GTNNAC 7 cut(s) 666, 764, 1131, 1209, 1553, 1615, 2240
Hpy99I CGWCG 2 cut(s) 372, 1365
HpyAV CCTTC 6 cut(s) 797, 881, 930, 1178, 2072, 2163
HpyCH4III ACNGT 7 cut(s) 700, 761, 956, 1120, 1512, 1646, 2189
HpyCH4IV ACGT 3 cut(s) 1027, 1205, 1211
HpyF10VI GCNNNNNNNGC 2 cut(s) 1380, 1712
HpyF3I CTNAG 6 cut(s) 602, 750, 1430, 1476, 1972, 2228
HpySE526I ACGT 3 cut(s) 1027, 1205, 1211
Hsp92I GRCGYC 1 cut(s) 370
KpnI GGTACC 1 cut(s) 993
Ksp22I TGATCA 2 cut(s) 400, 2047
KspAI GTTAAC 1 cut(s) 666
LmnI GCTCC 3 cut(s) 643, 1265, 2300
Lsp1109I GCAGC 5 cut(s) 17, 1097, 1841, 2030, 2115
LweI GCATC 9 cut(s) 80, 315, 343, 1091, 1141, 1732, 1951, 2092, 2103
MabI ACCWGGT 1 cut(s) 657
MaeI CTAG 6 cut(s) 59, 464, 521, 774, 1371, 1857
MaeII ACGT 3 cut(s) 1027, 1205, 1211
MaeIII GTNAC 5 cut(s) 405, 776, 1086, 1859, 2093
MbiI CCGCTC 1 cut(s) 1068
MboII GAAGA 6 cut(s) 278, 445, 887, 943, 1169, 2244
MfeI CAATTG 1 cut(s) 1608
MflI RGATCY 3 cut(s) 1004, 1571, 2317
MhlI GDGCHC 4 cut(s) 1133, 1537, 1700, 2242
MlyI GAGTC 4 cut(s) 20, 371, 1964, 2167
Mph1103I ATGCAT 1 cut(s) 1520
MroXI GAANNNNTTC 1 cut(s) 1101
MseI TTAA 7 cut(s) 428, 516, 612, 665, 1092, 1143, 1991
MslI CAYNNNNRTG 2 cut(s) 396, 1959
MspA1I CMGCKG 1 cut(s) 71
MspI CCGG 2 cut(s) 1313, 1481
MspR9I CCNGG 3 cut(s) 659, 1314, 1443
MunI CAATTG 1 cut(s) 1608
Mva1269I GAATGC 1 cut(s) 497
MvaI CCWGG 2 cut(s) 659, 1443
MwoI GCNNNNNNNGC 2 cut(s) 1380, 1712
NciI CCSGG 1 cut(s) 1314
NcoI CCATGG 2 cut(s) 999, 2164
NdeI CATATG 1 cut(s) 670
NheI GCTAGC 1 cut(s) 1370
NlaIV GGNNCC 5 cut(s) 645, 991, 1267, 1602, 2140
NmuCI GTSAC 3 cut(s) 405, 1086, 2093
NsiI ATGCAT 1 cut(s) 1520
NspI RCATGY 4 cut(s) 147, 308, 395, 2025
PaeR7I CTCGAG 2 cut(s) 20, 1328
PciI ACATGT 1 cut(s) 391
PctI GAATGC 1 cut(s) 497
PdmI GAANNNNTTC 1 cut(s) 1101
PfoI TCCNGGA 1 cut(s) 1312
PkrI GCNGC 8 cut(s) 7, 130, 374, 512, 1112, 1831, 2020, 2130
Ple19I CGATCG 1 cut(s) 908
PleI GAGTC 4 cut(s) 20, 370, 1964, 2167
PpsI GAGTC 4 cut(s) 20, 370, 1964, 2167
PscI ACATGT 1 cut(s) 391
Psp6I CCWGG 2 cut(s) 657, 1441
PspFI CCCAGC 1 cut(s) 616
PspGI CCWGG 2 cut(s) 657, 1441
PspN4I GGNNCC 5 cut(s) 645, 991, 1267, 1602, 2140
PspPI GGNCC 1 cut(s) 227
PspXI VCTCGAGB 1 cut(s) 20
PstI CTGCAG 2 cut(s) 796, 2198
PsuI RGATCY 3 cut(s) 1004, 1571, 2317
PvuI CGATCG 1 cut(s) 908
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 6 cut(s) 234, 948, 991, 1208, 1554, 2277
RsaNI GTAC 6 cut(s) 233, 947, 990, 1207, 1553, 2276
RseI CAYNNNNRTG 2 cut(s) 396, 1959
SaqAI TTAA 7 cut(s) 428, 516, 612, 665, 1092, 1143, 1991
SatI GCNGC 8 cut(s) 6, 129, 373, 511, 1111, 1830, 2019, 2129
Sau96I GGNCC 1 cut(s) 227
ScaI AGTACT 1 cut(s) 234
SchI GAGTC 4 cut(s) 20, 371, 1964, 2167
ScrFI CCNGG 3 cut(s) 659, 1314, 1443
SduI GDGCHC 4 cut(s) 1133, 1537, 1700, 2242
SexAI ACCWGGT 1 cut(s) 657
SfaNI GCATC 9 cut(s) 80, 315, 343, 1091, 1141, 1732, 1951, 2092, 2103
SfcI CTRYAG 2 cut(s) 792, 2194
Sfr274I CTCGAG 2 cut(s) 20, 1328
SinI GGWCC 1 cut(s) 227
SlaI CTCGAG 2 cut(s) 20, 1328
SmiMI CAYNNNNRTG 2 cut(s) 396, 1959
SmlI CTYRAG 3 cut(s) 20, 1008, 1328
SmoI CTYRAG 3 cut(s) 20, 1008, 1328
SpeI ACTAGT 1 cut(s) 1856
SsiI CCGC 5 cut(s) 128, 373, 511, 1047, 1066
SspI AATATT 1 cut(s) 541
SspMI CTAG 6 cut(s) 59, 464, 521, 774, 1371, 1857
StyD4I CCNGG 3 cut(s) 657, 1312, 1441
StyI CCWWGG 2 cut(s) 999, 2164
TaaI ACNGT 7 cut(s) 700, 761, 956, 1120, 1512, 1646, 2189
TaiI ACGT 3 cut(s) 1030, 1208, 1214
TaqI TCGA 9 cut(s) 21, 259, 501, 1171, 1199, 1329, 1363, 1911, 2118
TatI WGTACW 2 cut(s) 232, 1552
TauI GCSGC 3 cut(s) 131, 375, 513
Tru1I TTAA 7 cut(s) 428, 516, 612, 665, 1092, 1143, 1991
Tru9I TTAA 7 cut(s) 428, 516, 612, 665, 1092, 1143, 1991
TscAI CASTG 2 cut(s) 2192, 2203
TseFI GTSAC 3 cut(s) 405, 1086, 2093
TseI GCWGC 5 cut(s) 5, 1110, 1829, 2018, 2128
Tsp45I GTSAC 3 cut(s) 405, 1086, 2093
TspGWI ACGGA 2 cut(s) 1405, 2324
TspRI CASTG 2 cut(s) 2192, 2203
VneI GTGCAC 2 cut(s) 1129, 2238
VpaK11BI GGWCC 1 cut(s) 227
XapI RAATTY 7 cut(s) 242, 526, 563, 734, 913, 1285, 1761
XbaI TCTAGA 1 cut(s) 58
XceI RCATGY 4 cut(s) 147, 308, 395, 2025
XhoI CTCGAG 2 cut(s) 20, 1328
XmnI GAANNNNTTC 1 cut(s) 1101
XspI CTAG 6 cut(s) 59, 464, 521, 774, 1371, 1857
ZrmI AGTACT 1 cut(s) 234
Zsp2I ATGCAT 1 cut(s) 1520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.