Rh4CG458500

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0007229
Physical Location & Seq
Reverse (-)
68986217 .. 68997973
11757 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG458500.1

Sequence Viewer

Length: 2223 bp
ATGGCGGCTCTTGGAGAAGCATGTAATGGTGACACCACTTTTGCAGATTCATTGGAAGCATTTGGTGGTGATCTGGATGATCCTATAAGTGTTGCAATAGGAGGTCCAGTACTATCCAAATTCATTTCTGCATTTCGAGAGCTTGCTACATATAAAGAGCTTCTTCGCACCCAAGTAGAGCATGTGTTAGTCAATCGGTTGATGCACTTTATGTCAGTTGATCTGCAAGATGCAAAGGAGTCCCGACGCCGCTTTGACAAGGCAATACATGCTTATGATCAGTCACGGGAAAAGTTTGTATCCTTAAAGAAGAATACACGGGGAGACATTGTTTCTGAACTAGAGGAGGATCTACAAAATTCAAAATCAGCATTCGAGAAAGGCCGCTTCAACCTAGTAAATTCCCTAATGAATATTGAAGCAAAAAAGAAGTATGAATTTTTGGAATCTATTAGTGCAATCATGGATGGTCATCTAAGATACTTTAAACTGGGATATGAGTTATTGAGCCAAATGGAGCCTTATATTCACCAGGTGTTGACATATGCACAACAATCTAAAGAACAGGCTACTGTTGAACAAGATAAACTTCACAAAAGGATTCAGGAATTTAGGACACAAGCTGAGATGGATAGTATACGAGCTTCCAGTAACTTTGAGGCTTCTGCAGTGGTTGAAGGACATCGTGCCTTTGGTTTGGCTTCATACAAAAACGTAGAACCAATCACCTTTGAAAATGGGAAGCCACAGACAATCAAGGAAGGATATCTACTGAAACGATCGTCAAATTTGAGGGGAGACTGGAAGAGAAGGTTTTTTGTACTGAACAGTCAAGGGGCTTTGTTTTATTACAGAATCAAGGGTACCAAACCCATGGGATCTCAAGCACATCACTTCACACGTTCAACTGAACATAATAGCGGTGTTTTCGGGAGATTCCGCTCAAAAAATAGGGCATCGTCACTTAATGAAAACATTCTGGGCTGCCGAACTGTTGATCTGTGCACCTCAACAATTAAGATGGATGCAGAAGATACAGACCTTCGACTTTGCTTCAGAATAATATCCCCATCGAAAACGTACACATTGCAGGCTGAAAATGAGGCCGATAGGATGGATTGGATGAACAAAATAACAGGAGCTATTACATCGCTGCTGAATTTTCGGCTTCTTGAACAGCCACATCCGGGAAATGCGAATCTTGAGAGTTGTGAATTCGGTGTTGGTGATGTTTGTCGAGTAGCTAGCTGTGAAAGCTTACATAACATACAATTCAATAGAGTTGATTGTGTTTCTACTGCTCTTAGGGGAATCCCTGGAAATGATCTTTGTGCAGAGTGCAGTAGTCCTGAGCCTGATTGGGCATCTCTTAATCTTGGGATACTGTTATGCATTGAATGTTCTGGTGCTCATCGGAATCTTGGTGTACATATTTCAAAGGTGAGATCTATAACATTAGATGTCAAAGTTTGGGAACCGACAATTGTGGACTTATTTCGCAACTTGGGTAATGCTTACTGTAATTCTTTGTGGGAGGGAAAGCTTCTACTCAAAAATGAAAGATTGGATGGCCCAAATGCCATTAGGGCTTCAATTTCAAAACCATGTCCCCAAGATGCAATTCAGCATAAGGAAATTTACATTCAAGCAAAGTATGTAGAGAAGGTGATGGTCATTAGAGATGTGGCTGTACCTGTCCTTGCAGCAAGTATATGGGAAGCAGTCAAGACTAGTAACTTACAAGAAGTCTATCGGCTTATTGTGATATCAGATATGAATATTGTCGACACCACCTATGATGATGTGGTTGGCGACAATTTATATCACCATGTTGATGCACAAGACTCAGAGTTGAGTTTTCCCCCAATAGAGAGCAAACAGCACGATCCAGCAGCATGTGAGAGAATCAAGACTTCCAATGATCAAGGGAACTGCCTTCAAGGTTGTTCATTATTGCATCTGGCTTGTCACTCTGGCAATGCAATGATGCTTGAATTGTTGCTGCAATTTGGTGCCAACATAAATAGGTGTGATTTCCATGGAAGGACTCCTTTACACCACTGTATCTGCAGCGGGAATAATTCGCTGGCGAAGTTTCTACTTAGAAGAGGTGCACGCCCATCTATTCAAGATGGAGGAGGGCAAAGCGTACTGGAAAGAGCAATGGAGATGGGAGCGATTACGGATGAGGATTTATTCATAAAACTTTCTGAACATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_5 ArfGap BAR_3 PH

Protein Analysis

740

Amino Acids

83.08

Weight (kDa)

6.15

Isoelectric Point (pI)

44.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BAR_3 PF16746 6 - 197 2.6e-28 BAR domain of APPL family
BAR PF03114 12 - 176 1.5e-10 BAR domain
PH PF00169 252 - 382 2.1e-13 PH domain
ArfGap PF01412 434 - 557 3.2e-30 Putative GTPase activating protein for Arf
Ank_2 PF12796 640 - 711 6.3e-13 Ankyrin repeats (3 copies)
Ank_5 PF13857 644 - 687 7.4e-06 Ankyrin repeats (many copies)
Ank PF00023 648 - 678 3.3e-06 Ankyrin repeat
Ank_4 PF13637 652 - 701 2.8e-07 Ankyrin repeats (many copies)
Ank PF00023 680 - 711 7.7e-06 Ankyrin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 863
AccB1I GGYRCC 2 cut(s) 863, 2012
AccBSI CCGCTC 1 cut(s) 942
AccI GTMKAC 2 cut(s) 637, 1785
AciI CCGC 6 cut(s) 5, 250, 385, 921, 940, 2073
AclWI GGATC 4 cut(s) 74, 357, 886, 1880
AcsI RAATTY 9 cut(s) 119, 358, 400, 437, 608, 787, 1159, 1214, 1635
AcuI CTGAAG 1 cut(s) 1039
AcyI GRCGYC 1 cut(s) 247
AdeI CACNNNGTG 1 cut(s) 535
AfaI GTAC 7 cut(s) 111, 822, 865, 1082, 1428, 1692, 2151
AfiI CCNNNNNNNGG 1 cut(s) 1187
AflIII ACRYGT 1 cut(s) 899
AhlI ACTAGT 1 cut(s) 1730
AjnI CCWGG 2 cut(s) 531, 1315
AjuI GAANNNNNNNTTGG 4 cut(s) 1206, 1238, 1605, 1637
AluBI AGCT 9 cut(s) 142, 160, 623, 644, 1142, 1244, 1248, 1257, 1543
AluI AGCT 9 cut(s) 142, 160, 623, 644, 1142, 1244, 1248, 1257, 1543
Alw21I GWGCWC 3 cut(s) 1007, 1411, 2116
Alw26I GTCTC 2 cut(s) 318, 792
Alw44I GTGCAC 2 cut(s) 1003, 2112
AlwI GGATC 4 cut(s) 74, 357, 886, 1880
AoxI GGCC 3 cut(s) 382, 1104, 1570
ApaLI GTGCAC 2 cut(s) 1003, 2112
ApeKI GCWGC 6 cut(s) 984, 1153, 1703, 1892, 2002, 2070
ApoI RAATTY 9 cut(s) 119, 358, 400, 437, 608, 787, 1159, 1214, 1635
ArsI GACNNNNNNTTYG 2 cut(s) 23, 55
Asp700I GAANNNNTTC 1 cut(s) 975
Asp718I GGTACC 1 cut(s) 863
AspS9I GGNCC 2 cut(s) 104, 1571
AsuC2I CCSGG 1 cut(s) 1188
AsuHPI GGTGA 8 cut(s) 41, 80, 521, 718, 1238, 1453, 1678, 1817
AsuNHI GCTAGC 1 cut(s) 1244
AvaII GGWCC 1 cut(s) 104
BaeGI GKGCMC 2 cut(s) 1007, 2116
BaeI ACNNNNGTAYC 2 cut(s) 2047, 2080
BanI GGYRCC 2 cut(s) 863, 2012
BarI GAAGNNNNNNTAC 4 cut(s) 628, 660, 753, 785
Bbv12I GWGCWC 3 cut(s) 1007, 1411, 2116
BbvI GCAGC 6 cut(s) 971, 1140, 1715, 1904, 1989, 2082
BciT130I CCWGG 2 cut(s) 533, 1317
BciVI GTATCC 2 cut(s) 310, 1374
BclI TGATCA 2 cut(s) 277, 1921
BcnI CCSGG 1 cut(s) 1188
BcoDI GTCTC 2 cut(s) 318, 792
BcuI ACTAGT 1 cut(s) 1730
BfaI CTAG 4 cut(s) 341, 395, 1245, 1731
BfmI CTRYAG 2 cut(s) 666, 2068
BfuI GTATCC 2 cut(s) 310, 1374
BglI GCCNNNNNGGC 1 cut(s) 1586
BglII AGATCT 1 cut(s) 1445
BisI GCNGC 9 cut(s) 6, 250, 385, 985, 1154, 1704, 1893, 2003, 2071
BlsI GCNGC 9 cut(s) 7, 251, 386, 986, 1155, 1705, 1894, 2004, 2072
BmcAI AGTACT 1 cut(s) 111
Bme1390I CCNGG 3 cut(s) 533, 1188, 1317
Bme18I GGWCC 1 cut(s) 104
BmgT120I GGNCC 2 cut(s) 104, 1571
BmiI GGNNCC 4 cut(s) 519, 865, 1476, 2014
BmrFI CCNGG 3 cut(s) 533, 1188, 1317
BmrI ACTGGG 1 cut(s) 500
BmsI GCATC 9 cut(s) 192, 220, 965, 1015, 1373, 1606, 1825, 1966, 1977
BmtI GCTAGC 1 cut(s) 1248
BmuI ACTGGG 1 cut(s) 500
Bpu10I CCTNAGC 1 cut(s) 1350
BpuEI CTTGAG 2 cut(s) 867, 1223
BpuMI CCSGG 1 cut(s) 1188
BsaBI GATNNNNATC 1 cut(s) 471
BsaHI GRCGYC 1 cut(s) 247
BsaJI CCNNGG 3 cut(s) 873, 1315, 2038
BsaXI ACNNNNNCTCC 4 cut(s) 93, 123, 315, 345
Bsc4I CCNNNNNNNGG 1 cut(s) 1187
Bse1I ACTGG 5 cut(s) 107, 495, 648, 806, 2157
Bse3DI GCAATG 4 cut(s) 1085, 1984, 1989, 2169
Bse8I GATNNNNATC 1 cut(s) 471
BseBI CCWGG 2 cut(s) 533, 1317
BseDI CCNNGG 3 cut(s) 873, 1315, 2038
BseGI GGATG 8 cut(s) 82, 472, 1030, 1119, 1128, 1183, 1573, 2191
BseJI GATNNNNATC 1 cut(s) 471
BseLI CCNNNNNNNGG 1 cut(s) 1187
BseMI GCAATG 4 cut(s) 1085, 1984, 1989, 2169
BseMII CTCAG 3 cut(s) 615, 1341, 1860
BseNI ACTGG 5 cut(s) 107, 495, 648, 806, 2157
BseRI GAGGAG 2 cut(s) 359, 2151
BseSI GKGCMC 2 cut(s) 1007, 2116
BseXI GCAGC 6 cut(s) 971, 1140, 1715, 1904, 1989, 2082
BsgI GTGCAG 2 cut(s) 1353, 1360
Bsh1285I CGRYCG 1 cut(s) 782
BshFI GGCC 3 cut(s) 384, 1106, 1572
BshNI GGYRCC 2 cut(s) 863, 2012
BsiEI CGRYCG 1 cut(s) 782
BsiHKAI GWGCWC 3 cut(s) 1007, 1411, 2116
BsiSI CCGG 1 cut(s) 1187
BslFI GGGAC 2 cut(s) 226, 1593
BslI CCNNNNNNNGG 1 cut(s) 1187
BsmAI GTCTC 2 cut(s) 318, 792
BsmFI GGGAC 2 cut(s) 226, 1593
BsmI GAATGC 1 cut(s) 371
BsnI GGCC 3 cut(s) 384, 1106, 1572
Bsp1286I GDGCHC 3 cut(s) 1007, 1411, 2116
Bsp1407I TGTACA 1 cut(s) 1426
Bsp19I CCATGG 2 cut(s) 873, 2038
BspACI CCGC 6 cut(s) 5, 250, 385, 921, 940, 2073
BspANI GGCC 3 cut(s) 384, 1106, 1572
BspCNI CTCAG 3 cut(s) 616, 1342, 1859
BspLI GGNNCC 4 cut(s) 519, 865, 1476, 2014
BspMAI CTGCAG 2 cut(s) 670, 2072
BspOI GCTAGC 1 cut(s) 1248
BspPI GGATC 4 cut(s) 74, 357, 886, 1880
BspT107I GGYRCC 2 cut(s) 863, 2012
BsrBI CCGCTC 1 cut(s) 942
BsrDI GCAATG 4 cut(s) 1085, 1984, 1989, 2169
BsrGI TGTACA 1 cut(s) 1426
BsrI ACTGG 5 cut(s) 107, 495, 648, 806, 2157
BssECI CCNNGG 3 cut(s) 873, 1315, 2038
BssNAI GTATAC 1 cut(s) 638
BssNI GRCGYC 1 cut(s) 247
BssT1I CCWWGG 2 cut(s) 873, 2038
Bst1107I GTATAC 1 cut(s) 638
Bst2UI CCWGG 2 cut(s) 533, 1317
Bst4CI ACNGT 6 cut(s) 574, 830, 994, 1386, 1520, 2063
Bst6I CTCTTC 2 cut(s) 800, 2101
BstACI GRCGYC 1 cut(s) 247
BstAPI GCANNNNNTGC 1 cut(s) 269
BstAUI TGTACA 1 cut(s) 1426
BstC8I GCNNGC 5 cut(s) 144, 1092, 1246, 2088, 2116
BstDEI CTNAG 6 cut(s) 476, 624, 1304, 1350, 1846, 2102
BstDSI CCRYGG 2 cut(s) 873, 2038
BstF5I GGATG 8 cut(s) 82, 472, 1030, 1119, 1128, 1183, 1573, 2191
BstMAI GTCTC 2 cut(s) 318, 792
BstMCI CGRYCG 1 cut(s) 782
BstMWI GCNNNNNNNGC 3 cut(s) 269, 1254, 1586
BstNI CCWGG 2 cut(s) 533, 1317
BstNSI RCATGY 4 cut(s) 24, 185, 272, 1899
BstSCI CCNGG 3 cut(s) 531, 1186, 1315
BstSFI CTRYAG 2 cut(s) 666, 2068
BstSLI GKGCMC 2 cut(s) 1007, 2116
BstV1I GCAGC 6 cut(s) 971, 1140, 1715, 1904, 1989, 2082
BstX2I RGATCY 3 cut(s) 349, 878, 1445
BstXI CCANNNNNNTGG 1 cut(s) 874
BstYI RGATCY 3 cut(s) 349, 878, 1445
BstZ17I GTATAC 1 cut(s) 638
BsuI GTATCC 2 cut(s) 310, 1374
BsuRI GGCC 3 cut(s) 384, 1106, 1572
BtgI CCRYGG 2 cut(s) 873, 2038
BtgZI GCGATG 1 cut(s) 1134
BtsCI GGATG 8 cut(s) 82, 472, 1030, 1119, 1128, 1183, 1573, 2191
BtsI GCAGTG 1 cut(s) 675
BtsIMutI CAGTG 2 cut(s) 675, 2059
Cac8I GCNNGC 5 cut(s) 144, 1092, 1246, 2088, 2116
Cfr13I GGNCC 2 cut(s) 104, 1571
CseI GACGC 1 cut(s) 255
CsiI ACCWGGT 1 cut(s) 531
Csp6I GTAC 7 cut(s) 110, 821, 864, 1081, 1427, 1691, 2150
CviQI GTAC 7 cut(s) 110, 821, 864, 1081, 1427, 1691, 2150
DdeI CTNAG 6 cut(s) 476, 624, 1304, 1350, 1846, 2102
DraI TTTAAA 1 cut(s) 487
DraIII CACNNNGTG 1 cut(s) 535
Eam1104I CTCTTC 2 cut(s) 800, 2101
EarI CTCTTC 2 cut(s) 800, 2101
Eco130I CCWWGG 2 cut(s) 873, 2038
Eco32I GATATC 2 cut(s) 767, 1767
Eco47I GGWCC 1 cut(s) 104
Eco57I CTGAAG 1 cut(s) 1039
EcoRI GAATTC 1 cut(s) 1214
EcoRII CCWGG 2 cut(s) 531, 1315
EcoRV GATATC 2 cut(s) 767, 1767
EcoT14I CCWWGG 2 cut(s) 873, 2038
EcoT22I ATGCAT 1 cut(s) 1394
ErhI CCWWGG 2 cut(s) 873, 2038
FalI AAGNNNNNCTT 6 cut(s) 147, 179, 573, 605, 2035, 2067
FaqI GGGAC 2 cut(s) 226, 1593
FauI CCCGC 1 cut(s) 2066
FauNDI CATATG 1 cut(s) 544
FbaI TGATCA 2 cut(s) 277, 1921
FblI GTMKAC 2 cut(s) 637, 1785
Fnu4HI GCNGC 9 cut(s) 6, 250, 385, 985, 1154, 1704, 1893, 2003, 2071
FokI GGATG 8 cut(s) 89, 479, 1037, 1126, 1135, 1170, 1580, 2198
Fsp4HI GCNGC 9 cut(s) 6, 250, 385, 985, 1154, 1704, 1893, 2003, 2071
FspBI CTAG 4 cut(s) 341, 395, 1245, 1731
GluI GCNGC 9 cut(s) 6, 250, 385, 985, 1154, 1704, 1893, 2003, 2071
HaeIII GGCC 3 cut(s) 384, 1106, 1572
HapII CCGG 1 cut(s) 1187
HgaI GACGC 1 cut(s) 255
Hin1I GRCGYC 1 cut(s) 247
HincII GTYRAC 2 cut(s) 540, 1786
HindII GTYRAC 2 cut(s) 540, 1786
HindIII AAGCTT 2 cut(s) 1255, 1541
HpaII CCGG 1 cut(s) 1187
HphI GGTGA 8 cut(s) 41, 80, 521, 718, 1238, 1453, 1678, 1817
Hpy166II GTNNAC 8 cut(s) 540, 638, 1005, 1083, 1427, 1489, 1786, 2114
Hpy188I TCNGA 6 cut(s) 337, 1058, 1416, 1771, 1849, 2212
Hpy8I GTNNAC 8 cut(s) 540, 638, 1005, 1083, 1427, 1489, 1786, 2114
Hpy99I CGWCG 1 cut(s) 249
HpyAV CCTTC 7 cut(s) 671, 755, 804, 1052, 1657, 1946, 2037
HpyCH4III ACNGT 6 cut(s) 574, 830, 994, 1386, 1520, 2063
HpyCH4IV ACGT 3 cut(s) 714, 901, 1079
HpyF10VI GCNNNNNNNGC 3 cut(s) 269, 1254, 1586
HpyF3I CTNAG 6 cut(s) 476, 624, 1304, 1350, 1846, 2102
HpySE526I ACGT 3 cut(s) 714, 901, 1079
Hsp92I GRCGYC 1 cut(s) 247
KpnI GGTACC 1 cut(s) 867
Ksp22I TGATCA 2 cut(s) 277, 1921
LmnI GCTCC 3 cut(s) 517, 1139, 2174
Lsp1109I GCAGC 6 cut(s) 971, 1140, 1715, 1904, 1989, 2082
LweI GCATC 9 cut(s) 192, 220, 965, 1015, 1373, 1606, 1825, 1966, 1977
MabI ACCWGGT 1 cut(s) 531
MaeI CTAG 4 cut(s) 341, 395, 1245, 1731
MaeII ACGT 3 cut(s) 714, 901, 1079
MaeIII GTNAC 6 cut(s) 29, 282, 650, 960, 1733, 1967
MbiI CCGCTC 1 cut(s) 942
MboII GAAGA 5 cut(s) 155, 322, 817, 1043, 2118
MfeI CAATTG 1 cut(s) 1482
MflI RGATCY 3 cut(s) 349, 878, 1445
MhlI GDGCHC 3 cut(s) 1007, 1411, 2116
MlyI GAGTC 3 cut(s) 248, 1838, 2041
Mph1103I ATGCAT 1 cut(s) 1394
MroXI GAANNNNTTC 1 cut(s) 975
MseI TTAA 5 cut(s) 305, 486, 966, 1017, 1371
MslI CAYNNNNRTG 2 cut(s) 273, 1833
MspA1I CMGCKG 1 cut(s) 2073
MspI CCGG 1 cut(s) 1187
MspR9I CCNGG 3 cut(s) 533, 1188, 1317
MunI CAATTG 1 cut(s) 1482
Mva1269I GAATGC 1 cut(s) 371
MvaI CCWGG 2 cut(s) 533, 1317
MwoI GCNNNNNNNGC 3 cut(s) 269, 1254, 1586
NciI CCSGG 1 cut(s) 1188
NcoI CCATGG 2 cut(s) 873, 2038
NdeI CATATG 1 cut(s) 544
NheI GCTAGC 1 cut(s) 1244
NlaIV GGNNCC 4 cut(s) 519, 865, 1476, 2014
NmuCI GTSAC 4 cut(s) 29, 282, 960, 1967
NsiI ATGCAT 1 cut(s) 1394
NspI RCATGY 4 cut(s) 24, 185, 272, 1899
PctI GAATGC 1 cut(s) 371
PdmI GAANNNNTTC 1 cut(s) 975
PfeI GAWTC 9 cut(s) 47, 446, 601, 855, 936, 1198, 1311, 1417, 1905
PfoI TCCNGGA 1 cut(s) 1186
PkrI GCNGC 9 cut(s) 7, 251, 386, 986, 1155, 1705, 1894, 2004, 2072
Ple19I CGATCG 1 cut(s) 782
PleI GAGTC 3 cut(s) 247, 1838, 2041
PpsI GAGTC 3 cut(s) 247, 1838, 2041
Psp6I CCWGG 2 cut(s) 531, 1315
PspGI CCWGG 2 cut(s) 531, 1315
PspN4I GGNNCC 4 cut(s) 519, 865, 1476, 2014
PspPI GGNCC 2 cut(s) 104, 1571
PstI CTGCAG 2 cut(s) 670, 2072
PsuI RGATCY 3 cut(s) 349, 878, 1445
PvuI CGATCG 1 cut(s) 782
RsaI GTAC 7 cut(s) 111, 822, 865, 1082, 1428, 1692, 2151
RsaNI GTAC 7 cut(s) 110, 821, 864, 1081, 1427, 1691, 2150
RseI CAYNNNNRTG 2 cut(s) 273, 1833
SalI GTCGAC 1 cut(s) 1784
SaqAI TTAA 5 cut(s) 305, 486, 966, 1017, 1371
SatI GCNGC 9 cut(s) 6, 250, 385, 985, 1154, 1704, 1893, 2003, 2071
Sau96I GGNCC 2 cut(s) 104, 1571
ScaI AGTACT 1 cut(s) 111
SchI GAGTC 3 cut(s) 248, 1838, 2041
ScrFI CCNGG 3 cut(s) 533, 1188, 1317
SduI GDGCHC 3 cut(s) 1007, 1411, 2116
SexAI ACCWGGT 1 cut(s) 531
SfaNI GCATC 9 cut(s) 192, 220, 965, 1015, 1373, 1606, 1825, 1966, 1977
SfcI CTRYAG 2 cut(s) 666, 2068
SinI GGWCC 1 cut(s) 104
SmiMI CAYNNNNRTG 2 cut(s) 273, 1833
SmlI CTYRAG 2 cut(s) 882, 1202
SmoI CTYRAG 2 cut(s) 882, 1202
SpeI ACTAGT 1 cut(s) 1730
SsiI CCGC 6 cut(s) 5, 250, 385, 921, 940, 2073
SspI AATATT 2 cut(s) 415, 1780
SspMI CTAG 4 cut(s) 341, 395, 1245, 1731
StyD4I CCNGG 3 cut(s) 531, 1186, 1315
StyI CCWWGG 2 cut(s) 873, 2038
TaaI ACNGT 6 cut(s) 574, 830, 994, 1386, 1520, 2063
TaiI ACGT 3 cut(s) 717, 904, 1082
TaqI TCGA 6 cut(s) 136, 375, 1045, 1073, 1237, 1785
TatI WGTACW 3 cut(s) 109, 820, 1426
TauI GCSGC 3 cut(s) 8, 252, 387
TfiI GAWTC 9 cut(s) 47, 446, 601, 855, 936, 1198, 1311, 1417, 1905
Tru1I TTAA 5 cut(s) 305, 486, 966, 1017, 1371
Tru9I TTAA 5 cut(s) 305, 486, 966, 1017, 1371
TscAI CASTG 2 cut(s) 675, 2066
TseFI GTSAC 4 cut(s) 29, 282, 960, 1967
TseI GCWGC 6 cut(s) 984, 1153, 1703, 1892, 2002, 2070
Tsp45I GTSAC 4 cut(s) 29, 282, 960, 1967
TspGWI ACGGA 1 cut(s) 2198
TspRI CASTG 2 cut(s) 675, 2066
VneI GTGCAC 2 cut(s) 1003, 2112
VpaK11BI GGWCC 1 cut(s) 104
XapI RAATTY 9 cut(s) 119, 358, 400, 437, 608, 787, 1159, 1214, 1635
XceI RCATGY 4 cut(s) 24, 185, 272, 1899
XmiI GTMKAC 2 cut(s) 637, 1785
XmnI GAANNNNTTC 1 cut(s) 975
XspI CTAG 4 cut(s) 341, 395, 1245, 1731
ZrmI AGTACT 1 cut(s) 111
Zsp2I ATGCAT 1 cut(s) 1394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.