Rmu_sc0010778.1_g000001

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0010778.1
OG0007229
Physical Location & Seq
Reverse (-)
1 .. 4950
4950 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0010778.1_g000001.1.cds

Sequence Viewer

Length: 657 bp
atggcagcatttatcaagctcgaggactccccaatgttccagaagcaggtatgtaatctagaacagacagctgatgagttgaaagagcgatgccaaaaactattcaaagggtgtaagaagtttatggcggctcttggagaagcatgtaatggcgacaccacttttgcagattcattggaagcatttggtggtgatctggatgatcctataagtgttgcaataggaggtccagtactatccaaattcatttctgcatttcgagagcttgctacatataaagagcttcttcgcacccaagtagagcatgtgttagtcaatcggttgatgcactttatgtctgttgatctgcaagatgcaaaggagtcccgacgccgctttgacaaggcaatacatgtttatgatcagtcacgggaaaagtttgtatccttaaagaagaatacacggggagacattgtttctgaactagaggaggatctacaaaactcaaaatcagcattcgagaaaggccgctttaacctagtaaattccctaatgaatattgaagcaaaaaagaagtatgaatttttggaatctattagtgcaatcatggatggtcatctaagatactttaagctgggatatgagttattgagccaaatggagccatatattcaccag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_5 ArfGap BAR_3 PH

Protein Analysis

219

Amino Acids

25.11

Weight (kDa)

6.01

Isoelectric Point (pI)

41.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 37
AciI CCGC 3 cut(s) 128, 373, 508
AclWI GGATC 2 cut(s) 197, 480
AcsI RAATTY 3 cut(s) 242, 523, 560
AcyI GRCGYC 1 cut(s) 370
AfaI GTAC 1 cut(s) 234
AfiI CCNNNNNNNGG 1 cut(s) 46
AflIII ACRYGT 1 cut(s) 391
AgsI TTSAA 3 cut(s) 82, 106, 542
AluBI AGCT 5 cut(s) 19, 71, 265, 283, 613
AluI AGCT 5 cut(s) 19, 71, 265, 283, 613
Alw26I GTCTC 1 cut(s) 441
AlwI GGATC 2 cut(s) 197, 480
Ama87I CYCGRG 1 cut(s) 20
AoxI GGCC 1 cut(s) 505
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 3 cut(s) 242, 523, 560
ArsI GACNNNNNNTTYG 2 cut(s) 146, 178
AspS9I GGNCC 1 cut(s) 227
AsuHPI GGTGA 2 cut(s) 203, 644
AvaI CYCGRG 1 cut(s) 20
AvaII GGWCC 1 cut(s) 227
BbvI GCAGC 1 cut(s) 17
BccI CCATC 1 cut(s) 584
BciVI GTATCC 1 cut(s) 433
BclI TGATCA 1 cut(s) 400
BcoDI GTCTC 1 cut(s) 441
BfaI CTAG 3 cut(s) 59, 464, 518
BfuAI ACCTGC 1 cut(s) 37
BfuI GTATCC 1 cut(s) 433
BisI GCNGC 4 cut(s) 6, 129, 373, 508
BlsI GCNGC 4 cut(s) 7, 130, 374, 509
BmcAI AGTACT 1 cut(s) 234
Bme18I GGWCC 1 cut(s) 227
BmeT110I CYCGRG 1 cut(s) 20
BmgT120I GGNCC 1 cut(s) 227
BmiI GGNNCC 1 cut(s) 642
BmsI GCATC 3 cut(s) 80, 315, 343
BsaBI GATNNNNATC 1 cut(s) 594
BsaHI GRCGYC 1 cut(s) 370
BsaXI ACNNNNNCTCC 4 cut(s) 216, 246, 438, 468
Bsc4I CCNNNNNNNGG 1 cut(s) 46
Bse1I ACTGG 1 cut(s) 230
Bse8I GATNNNNATC 1 cut(s) 594
BseGI GGATG 2 cut(s) 205, 595
BseJI GATNNNNATC 1 cut(s) 594
BseLI CCNNNNNNNGG 1 cut(s) 46
BseNI ACTGG 1 cut(s) 230
BseRI GAGGAG 1 cut(s) 482
BseXI GCAGC 1 cut(s) 17
BseYI CCCAGC 1 cut(s) 613
BshFI GGCC 1 cut(s) 507
BsiHKCI CYCGRG 1 cut(s) 20
BslFI GGGAC 1 cut(s) 349
BslI CCNNNNNNNGG 1 cut(s) 46
BsmAI GTCTC 1 cut(s) 441
BsmFI GGGAC 1 cut(s) 349
BsmI GAATGC 1 cut(s) 494
BsnI GGCC 1 cut(s) 507
BsoBI CYCGRG 1 cut(s) 20
Bsp143I GATC 5 cut(s) 193, 202, 343, 400, 472
BspACI CCGC 3 cut(s) 128, 373, 508
BspANI GGCC 1 cut(s) 507
BspLI GGNNCC 1 cut(s) 642
BspMI ACCTGC 1 cut(s) 37
BspPI GGATC 2 cut(s) 197, 480
BsrI ACTGG 1 cut(s) 230
BssMI GATC 5 cut(s) 193, 202, 343, 400, 472
BssNI GRCGYC 1 cut(s) 370
BstACI GRCGYC 1 cut(s) 370
BstC8I GCNNGC 1 cut(s) 267
BstDEI CTNAG 1 cut(s) 599
BstF5I GGATG 2 cut(s) 205, 595
BstKTI GATC 5 cut(s) 196, 205, 346, 403, 475
BstMAI GTCTC 1 cut(s) 441
BstMBI GATC 5 cut(s) 193, 202, 343, 400, 472
BstNSI RCATGY 3 cut(s) 147, 308, 395
BstV1I GCAGC 1 cut(s) 17
BstX2I RGATCY 1 cut(s) 472
BstYI RGATCY 1 cut(s) 472
BsuI GTATCC 1 cut(s) 433
BsuRI GGCC 1 cut(s) 507
BtgZI GCGATG 1 cut(s) 103
BtsCI GGATG 2 cut(s) 205, 595
BveI ACCTGC 1 cut(s) 37
Cac8I GCNNGC 1 cut(s) 267
Cfr13I GGNCC 1 cut(s) 227
CseI GACGC 1 cut(s) 378
Csp6I GTAC 1 cut(s) 233
CviAII CATG 4 cut(s) 144, 305, 392, 586
CviJI RGCY 9 cut(s) 19, 71, 131, 265, 283, 507, 613, 633, 643
CviKI_1 RGCY 9 cut(s) 19, 71, 131, 265, 283, 507, 613, 633, 643
CviQI GTAC 1 cut(s) 233
DdeI CTNAG 1 cut(s) 599
DpnI GATC 5 cut(s) 195, 204, 345, 402, 474
DpnII GATC 5 cut(s) 193, 202, 343, 400, 472
Eco47I GGWCC 1 cut(s) 227
Eco88I CYCGRG 1 cut(s) 20
FaeI CATG 4 cut(s) 147, 308, 395, 589
FalI AAGNNNNNCTT 2 cut(s) 270, 302
FaqI GGGAC 1 cut(s) 349
FatI CATG 4 cut(s) 143, 304, 391, 585
FbaI TGATCA 1 cut(s) 400
Fnu4HI GCNGC 4 cut(s) 6, 129, 373, 508
FokI GGATG 2 cut(s) 212, 602
Fsp4HI GCNGC 4 cut(s) 6, 129, 373, 508
FspBI CTAG 3 cut(s) 59, 464, 518
GluI GCNGC 4 cut(s) 6, 129, 373, 508
GsaI CCCAGC 1 cut(s) 617
HaeIII GGCC 1 cut(s) 507
HgaI GACGC 1 cut(s) 378
Hin1I GRCGYC 1 cut(s) 370
Hin1II CATG 4 cut(s) 147, 308, 395, 589
HinfI GANTC 4 cut(s) 26, 170, 362, 569
HphI GGTGA 2 cut(s) 203, 644
Hpy188I TCNGA 1 cut(s) 460
Hpy188III TCNNGA 6 cut(s) 40, 59, 197, 260, 366, 499
Hpy99I CGWCG 1 cut(s) 372
HpyCH4V TGCA 7 cut(s) 167, 218, 254, 328, 349, 356, 581
HpyF3I CTNAG 1 cut(s) 599
Hsp92I GRCGYC 1 cut(s) 370
Hsp92II CATG 4 cut(s) 147, 308, 395, 589
Ksp22I TGATCA 1 cut(s) 400
Kzo9I GATC 5 cut(s) 193, 202, 343, 400, 472
LmnI GCTCC 1 cut(s) 640
LpnPI CCDG 5 cut(s) 32, 53, 182, 243, 599
Lsp1109I GCAGC 1 cut(s) 17
LweI GCATC 3 cut(s) 80, 315, 343
MaeI CTAG 3 cut(s) 59, 464, 518
MaeIII GTNAC 1 cut(s) 405
MalI GATC 5 cut(s) 195, 204, 345, 402, 474
MboI GATC 5 cut(s) 193, 202, 343, 400, 472
MboII GAAGA 2 cut(s) 278, 445
MflI RGATCY 1 cut(s) 472
MluCI AATT 3 cut(s) 242, 523, 560
MlyI GAGTC 2 cut(s) 20, 371
MnlI CCTC 4 cut(s) 16, 218, 460, 463
MseI TTAA 3 cut(s) 428, 513, 609
MslI CAYNNNNRTG 1 cut(s) 396
MspA1I CMGCKG 1 cut(s) 71
Mva1269I GAATGC 1 cut(s) 494
NdeII GATC 5 cut(s) 193, 202, 343, 400, 472
NlaIII CATG 4 cut(s) 147, 308, 395, 589
NlaIV GGNNCC 1 cut(s) 642
NmuCI GTSAC 1 cut(s) 405
NspI RCATGY 3 cut(s) 147, 308, 395
PaeR7I CTCGAG 1 cut(s) 20
PciI ACATGT 1 cut(s) 391
PctI GAATGC 1 cut(s) 494
PfeI GAWTC 2 cut(s) 170, 569
PkrI GCNGC 4 cut(s) 7, 130, 374, 509
PleI GAGTC 2 cut(s) 20, 370
PpsI GAGTC 2 cut(s) 20, 370
PscI ACATGT 1 cut(s) 391
PspFI CCCAGC 1 cut(s) 613
PspN4I GGNNCC 1 cut(s) 642
PspPI GGNCC 1 cut(s) 227
PspXI VCTCGAGB 1 cut(s) 20
PsuI RGATCY 1 cut(s) 472
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 1 cut(s) 234
RsaNI GTAC 1 cut(s) 233
RseI CAYNNNNRTG 1 cut(s) 396
SaqAI TTAA 3 cut(s) 428, 513, 609
SatI GCNGC 4 cut(s) 6, 129, 373, 508
Sau3AI GATC 5 cut(s) 193, 202, 343, 400, 472
Sau96I GGNCC 1 cut(s) 227
ScaI AGTACT 1 cut(s) 234
SchI GAGTC 2 cut(s) 20, 371
SetI ASST 8 cut(s) 21, 51, 73, 229, 267, 285, 519, 615
SfaNI GCATC 3 cut(s) 80, 315, 343
Sfr274I CTCGAG 1 cut(s) 20
SinI GGWCC 1 cut(s) 227
SlaI CTCGAG 1 cut(s) 20
SmiMI CAYNNNNRTG 1 cut(s) 396
SmlI CTYRAG 1 cut(s) 20
SmoI CTYRAG 1 cut(s) 20
Sse9I AATT 3 cut(s) 242, 523, 560
SsiI CCGC 3 cut(s) 128, 373, 508
SspI AATATT 1 cut(s) 538
SspMI CTAG 3 cut(s) 59, 464, 518
TaqI TCGA 3 cut(s) 21, 259, 498
TasI AATT 3 cut(s) 242, 523, 560
TatI WGTACW 1 cut(s) 232
TauI GCSGC 3 cut(s) 131, 375, 510
TfiI GAWTC 2 cut(s) 170, 569
Tru1I TTAA 3 cut(s) 428, 513, 609
Tru9I TTAA 3 cut(s) 428, 513, 609
TseFI GTSAC 1 cut(s) 405
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 1 cut(s) 405
TspDTI ATGAA 4 cut(s) 162, 235, 548, 573
VpaK11BI GGWCC 1 cut(s) 227
XapI RAATTY 3 cut(s) 242, 523, 560
XbaI TCTAGA 1 cut(s) 58
XceI RCATGY 3 cut(s) 147, 308, 395
XhoI CTCGAG 1 cut(s) 20
XspI CTAG 3 cut(s) 59, 464, 518
ZrmI AGTACT 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.