RchiOBHm_Chr4g0446341

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0007229
Physical Location & Seq
Reverse (-)
66548911 .. 66559520
10610 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41386

Sequence Viewer

Length: 2346 bp
ATGGCAGCATTTATCAAGCTCGAGGACTCCCCAATGTTCCAGAAGCAGGTATGTAATCTAGAACAGACAGCTGATGAGTTGAAAGAGCGATGCCAAAAACTATTCAAAGGGTGTAAGAAGTTTATGGCGGCTCTTGGAGAAGCATGTAATGGCGACACCACTTTTGCAGATTCATTGGAAGCATTTGGTGGTGATCTGGATGATCCTATAAGTGTTGCAATAGGAGGTCCAGTGCTATCCAAATTCATTTCTGCATTTCGAGAGCTTGCTACATATAAAGAGCTTCTTCGCACCCAAGTAGAGCATGTGTTAGTCAATCGGTTGATGCACTTTATGTCTGTTGATCTGCAAGATGCAAAGGAGTCCCGACGCCGCTTTGACAAGGCAATACATGTTTATGATCAGTCACGGGAAAAGTTTGTATCCTTAAAGAAGAATACACGGGGAGACATTGTTTCTGAACTAGAGGAGGATCTACAAAACTCAAAATCAGCATTCGAGAAAGGCCGCTTCAACCTAGTAAATTCCCTAATGAATATTGAAGCAAAAAAGAAGTATGAATTTTTGGAATCTATTAGTGCAATCATGGATGGTCATCTAAGATACTTTAAGCTGGGATATGAGTTATTGAGCCAAATGGAGCCATATATTCACCAGGTGTTAACATATGCACAACAATCTAAAGAACAGGCTACTGTTGAACAAGATAAACTTCACAAAAGGATTCAGGAATTTAGGACACAAGCTGAGATGGACAGTTTACAAGCTTCTAGTAACTTTGAGGCTTCTGCAGGGGTTGAAGGACATCGTGCCTTTGGTTTGGCTTCATACAAAAACACAGAACCAATCACCTTTGAAAATGGGAAGCCACAGACAATCAAGCAAGGATATCTTCTGAAACGATCGTCAAATTTGAGGGGAGACTGGAAGAGAAGGTTTTTCGTACTGAACAGTCAAGGGGCTTTATTTTATTACAGAATCAAGGGTACCAAACCCATGGGATCTCAAGCACATCACTTTACACGTTCAACTGAACATAATAGCGGTGTTTTCGGGAGATTCCGCTCAAAAAATAGGGCATCGTCACTTAATGAAAACATTCTGGGCTGCCGAACTGTTGATCTGTGCACTTCAACAATTAAGATGGATGCAGAAGATACAGACCTTCGACTTTGCTTCAGAATAATCTCTCCATCAAAAACGTACACGTTGCAGGCTGAAAATGAGGCCGATAGGATGGATTGGATGAACAAAATAACAGGAGCCATTACATCTCTGCTGAATTTTCGGCTTCTTGAACAGCCACATCCGGGAAATGCGAATCTCGAGGGTAGTGAATTAGGTGTTGGTGATGTTCGTCGAGTAGCTAGCTGTGAAAGCTTACATAACATACACTTCAATAGAGTTGATTCCGTTTCTACCGCTCTTAGGGGAATCCCTGGAAATGATCTTTGTGCAGAGTGCAGTAGTCCTGAGCCGGATTGGGCTTCTCTCAATCTTGGGATACTGTTATGCATTGAATGTTCTGGTGCTCATCGGAATCTTGGTGTACATATTTCAAAGGTGAGATCTATAACATTAGATGTCAAAGTTTGGGAACCGACAATTGTGGACTTATTTCGCAACTTGGGTAATGCCTACTGTAATTCTGTGTGGGAGGGAAAGCTTCAACTCAAAAACGAAAGATTGGAGGGCTCAAATGCCATTAGGGCTTCAATTTCAAAACCATGTCCCCAAGATGCAATTCAGCATAAGGAAATTTACATTCAAGCAAAGTATGTAGAGAAAGTGATGGTCATTAGAGATGTAGCTGTACCTTTCCTTGCAGCAAGTATATGGGAAGCAGTCAAGACTAGTAACTTACAAGAAGTCTATCGGCTTATTGTGATATCAGATATGAATATCGTCGATACCACCTATGATGATGTGGTTGGCGATGATTTATATCACCATGTTGATGCACAAGACTCAGAGTTGAGTTTTCCCTTAATAGAGAGCAAACAGCACGATCCAGCAGCATGTGAGAGAATCAAGACTTCCAATGATCAAGGGAACTGCCTTCAAGGTTGTTCATTATTGCATCTGGCTTGTCACTCTGGCAATGCAATGATGCTCGAATTGTTGCTGCAATTTGGTGCCAACATAAATAGGCGTGATTTCCATGGAAGGACTCCTTTACACCACTGTATCTGCAGTGGGAATAATTCGCTGGCGAAGTTTCTACTTAGAAGAGGTGCACGCCCATCAATTCAAGACGGAGGAGGGCAAAGCGTACTGGAAAGAGCAATGGAGATGGGAGCGATTACGGATGAGGATCTATTCATAAAACTTTCTGAACATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_5 ArfGap BAR_3 PH

Protein Analysis

781

Amino Acids

87.94

Weight (kDa)

6.36

Isoelectric Point (pI)

45.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BAR_3 PF16746 7 - 238 1.2e-37 BAR domain of APPL family
BAR PF03114 18 - 217 7.5e-13 BAR domain
PH PF00169 293 - 423 4.7e-13 PH domain
ArfGap PF01412 473 - 598 9e-31 Putative GTPase activating protein for Arf
Ank_2 PF12796 675 - 752 6e-13 Ankyrin repeats (3 copies)
Ank_5 PF13857 685 - 728 4.1e-06 Ankyrin repeats (many copies)
Ank PF00023 689 - 719 1.7e-06 Ankyrin repeat
Ank_4 PF13637 693 - 742 3.4e-07 Ankyrin repeats (many copies)
Ank PF00023 721 - 752 8.2e-06 Ankyrin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 37
Acc65I GGTACC 1 cut(s) 986
AccB1I GGYRCC 2 cut(s) 986, 2135
AccBSI CCGCTC 2 cut(s) 1065, 1424
AciI CCGC 6 cut(s) 128, 373, 508, 1044, 1063, 1422
AclWI GGATC 5 cut(s) 197, 480, 1009, 2003, 2322
AcsI RAATTY 7 cut(s) 242, 523, 560, 731, 910, 1282, 1758
AcuI CTGAAG 1 cut(s) 1162
AcyI GRCGYC 1 cut(s) 370
AdeI CACNNNGTG 1 cut(s) 658
AfaI GTAC 6 cut(s) 945, 988, 1205, 1551, 1815, 2274
AfiI CCNNNNNNNGG 3 cut(s) 46, 1310, 1428
AflIII ACRYGT 3 cut(s) 391, 1022, 1206
AhlI ACTAGT 1 cut(s) 1853
AjnI CCWGG 2 cut(s) 654, 1438
AjuI GAANNNNNNNTTGG 4 cut(s) 1329, 1361, 1728, 1760
Alw21I GWGCWC 3 cut(s) 1130, 1534, 2239
Alw26I GTCTC 2 cut(s) 441, 915
Alw44I GTGCAC 2 cut(s) 1126, 2235
AlwI GGATC 5 cut(s) 197, 480, 1009, 2003, 2322
Ama87I CYCGRG 2 cut(s) 20, 1325
AoxI GGCC 2 cut(s) 505, 1227
ApaLI GTGCAC 2 cut(s) 1126, 2235
ApeKI GCWGC 5 cut(s) 5, 1107, 1826, 2015, 2125
ApoI RAATTY 7 cut(s) 242, 523, 560, 731, 910, 1282, 1758
ArsI GACNNNNNNTTYG 2 cut(s) 146, 178
Asp700I GAANNNNTTC 1 cut(s) 1098
Asp718I GGTACC 1 cut(s) 986
AspS9I GGNCC 1 cut(s) 227
AsuC2I CCSGG 1 cut(s) 1311
AsuHPI GGTGA 6 cut(s) 203, 644, 841, 1361, 1576, 1940
AsuNHI GCTAGC 1 cut(s) 1367
AvaI CYCGRG 2 cut(s) 20, 1325
AvaII GGWCC 1 cut(s) 227
BaeGI GKGCMC 2 cut(s) 1130, 2239
BaeI ACNNNNGTAYC 2 cut(s) 2170, 2203
BanI GGYRCC 2 cut(s) 986, 2135
BanII GRGCYC 1 cut(s) 1697
Bbv12I GWGCWC 3 cut(s) 1130, 1534, 2239
BbvI GCAGC 5 cut(s) 17, 1094, 1838, 2027, 2112
BccI CCATC 8 cut(s) 584, 745, 1138, 1201, 1231, 1786, 2251, 2287
BciT130I CCWGG 2 cut(s) 656, 1440
BciVI GTATCC 2 cut(s) 433, 1497
BclI TGATCA 2 cut(s) 400, 2044
BcnI CCSGG 1 cut(s) 1311
BcoDI GTCTC 2 cut(s) 441, 915
BcuI ACTAGT 1 cut(s) 1853
BfaI CTAG 6 cut(s) 59, 464, 518, 771, 1368, 1854
BfmI CTRYAG 2 cut(s) 789, 2191
BfuAI ACCTGC 1 cut(s) 37
BfuI GTATCC 2 cut(s) 433, 1497
BglI GCCNNNNNGGC 1 cut(s) 1709
BglII AGATCT 1 cut(s) 1568
BisI GCNGC 8 cut(s) 6, 129, 373, 508, 1108, 1827, 2016, 2126
BlsI GCNGC 8 cut(s) 7, 130, 374, 509, 1109, 1828, 2017, 2127
Bme1390I CCNGG 3 cut(s) 656, 1311, 1440
Bme18I GGWCC 1 cut(s) 227
BmeT110I CYCGRG 2 cut(s) 20, 1325
BmgT120I GGNCC 1 cut(s) 227
BmiI GGNNCC 5 cut(s) 642, 988, 1264, 1599, 2137
BmrFI CCNGG 3 cut(s) 656, 1311, 1440
BmsI GCATC 9 cut(s) 80, 315, 343, 1088, 1138, 1729, 1948, 2089, 2100
BmtI GCTAGC 1 cut(s) 1371
Bpu10I CCTNAGC 1 cut(s) 1473
BpuEI CTTGAG 1 cut(s) 990
BpuMI CCSGG 1 cut(s) 1311
BsaBI GATNNNNATC 3 cut(s) 594, 1944, 2313
BsaHI GRCGYC 1 cut(s) 370
BsaJI CCNNGG 3 cut(s) 996, 1438, 2161
BsaXI ACNNNNNCTCC 4 cut(s) 216, 246, 438, 468
Bsc4I CCNNNNNNNGG 3 cut(s) 46, 1310, 1428
Bse1I ACTGG 3 cut(s) 230, 929, 2280
Bse3DI GCAATG 3 cut(s) 2107, 2112, 2292
Bse8I GATNNNNATC 3 cut(s) 594, 1944, 2313
BseBI CCWGG 2 cut(s) 656, 1440
BseDI CCNNGG 3 cut(s) 996, 1438, 2161
BseGI GGATG 7 cut(s) 205, 595, 1153, 1242, 1251, 1306, 2314
BseJI GATNNNNATC 3 cut(s) 594, 1944, 2313
BseLI CCNNNNNNNGG 3 cut(s) 46, 1310, 1428
BseMI GCAATG 3 cut(s) 2107, 2112, 2292
BseMII CTCAG 3 cut(s) 738, 1464, 1983
BseNI ACTGG 3 cut(s) 230, 929, 2280
BseRI GAGGAG 2 cut(s) 482, 2274
BseSI GKGCMC 2 cut(s) 1130, 2239
BseXI GCAGC 5 cut(s) 17, 1094, 1838, 2027, 2112
BseYI CCCAGC 1 cut(s) 613
BsgI GTGCAG 2 cut(s) 1476, 1483
Bsh1285I CGRYCG 1 cut(s) 905
BshFI GGCC 2 cut(s) 507, 1229
BshNI GGYRCC 2 cut(s) 986, 2135
BsiEI CGRYCG 1 cut(s) 905
BsiHKAI GWGCWC 3 cut(s) 1130, 1534, 2239
BsiHKCI CYCGRG 2 cut(s) 20, 1325
BsiSI CCGG 2 cut(s) 1310, 1478
BslFI GGGAC 2 cut(s) 349, 1716
BslI CCNNNNNNNGG 3 cut(s) 46, 1310, 1428
BsmAI GTCTC 2 cut(s) 441, 915
BsmFI GGGAC 2 cut(s) 349, 1716
BsmI GAATGC 1 cut(s) 494
BsnI GGCC 2 cut(s) 507, 1229
BsoBI CYCGRG 2 cut(s) 20, 1325
Bsp1286I GDGCHC 4 cut(s) 1130, 1534, 1697, 2239
Bsp1407I TGTACA 1 cut(s) 1549
Bsp19I CCATGG 2 cut(s) 996, 2161
BspACI CCGC 6 cut(s) 128, 373, 508, 1044, 1063, 1422
BspANI GGCC 2 cut(s) 507, 1229
BspCNI CTCAG 3 cut(s) 739, 1465, 1982
BspLI GGNNCC 5 cut(s) 642, 988, 1264, 1599, 2137
BspMAI CTGCAG 2 cut(s) 793, 2195
BspMI ACCTGC 1 cut(s) 37
BspOI GCTAGC 1 cut(s) 1371
BspPI GGATC 5 cut(s) 197, 480, 1009, 2003, 2322
BspT107I GGYRCC 2 cut(s) 986, 2135
BsrBI CCGCTC 2 cut(s) 1065, 1424
BsrDI GCAATG 3 cut(s) 2107, 2112, 2292
BsrGI TGTACA 1 cut(s) 1549
BsrI ACTGG 3 cut(s) 230, 929, 2280
BssECI CCNNGG 3 cut(s) 996, 1438, 2161
BssNI GRCGYC 1 cut(s) 370
BssT1I CCWWGG 2 cut(s) 996, 2161
Bst2UI CCWGG 2 cut(s) 656, 1440
Bst4CI ACNGT 7 cut(s) 697, 758, 953, 1117, 1509, 1643, 2186
Bst6I CTCTTC 2 cut(s) 923, 2224
BstACI GRCGYC 1 cut(s) 370
BstAUI TGTACA 1 cut(s) 1549
BstC8I GCNNGC 5 cut(s) 267, 1215, 1369, 2211, 2239
BstDEI CTNAG 6 cut(s) 599, 747, 1427, 1473, 1969, 2225
BstDSI CCRYGG 2 cut(s) 996, 2161
BstF5I GGATG 7 cut(s) 205, 595, 1153, 1242, 1251, 1306, 2314
BstMAI GTCTC 2 cut(s) 441, 915
BstMCI CGRYCG 1 cut(s) 905
BstMWI GCNNNNNNNGC 2 cut(s) 1377, 1709
BstNI CCWGG 2 cut(s) 656, 1440
BstNSI RCATGY 4 cut(s) 147, 308, 395, 2022
BstSCI CCNGG 3 cut(s) 654, 1309, 1438
BstSFI CTRYAG 2 cut(s) 789, 2191
BstSLI GKGCMC 2 cut(s) 1130, 2239
BstV1I GCAGC 5 cut(s) 17, 1094, 1838, 2027, 2112
BstX2I RGATCY 4 cut(s) 472, 1001, 1568, 2314
BstXI CCANNNNNNTGG 1 cut(s) 997
BstYI RGATCY 4 cut(s) 472, 1001, 1568, 2314
BsuI GTATCC 2 cut(s) 433, 1497
BsuRI GGCC 2 cut(s) 507, 1229
BtgI CCRYGG 2 cut(s) 996, 2161
BtgZI GCGATG 2 cut(s) 103, 1950
BtsCI GGATG 7 cut(s) 205, 595, 1153, 1242, 1251, 1306, 2314
BtsI GCAGTG 1 cut(s) 2200
BtsIMutI CAGTG 3 cut(s) 237, 2182, 2200
BveI ACCTGC 1 cut(s) 37
Cac8I GCNNGC 5 cut(s) 267, 1215, 1369, 2211, 2239
Cfr13I GGNCC 1 cut(s) 227
CseI GACGC 1 cut(s) 378
CsiI ACCWGGT 1 cut(s) 654
Csp6I GTAC 6 cut(s) 944, 987, 1204, 1550, 1814, 2273
CviQI GTAC 6 cut(s) 944, 987, 1204, 1550, 1814, 2273
DdeI CTNAG 6 cut(s) 599, 747, 1427, 1473, 1969, 2225
DraIII CACNNNGTG 1 cut(s) 658
Eam1104I CTCTTC 2 cut(s) 923, 2224
EarI CTCTTC 2 cut(s) 923, 2224
Eco130I CCWWGG 2 cut(s) 996, 2161
Eco24I GRGCYC 1 cut(s) 1697
Eco32I GATATC 2 cut(s) 890, 1890
Eco47I GGWCC 1 cut(s) 227
Eco57I CTGAAG 1 cut(s) 1162
Eco88I CYCGRG 2 cut(s) 20, 1325
EcoRII CCWGG 2 cut(s) 654, 1438
EcoRV GATATC 2 cut(s) 890, 1890
EcoT14I CCWWGG 2 cut(s) 996, 2161
EcoT22I ATGCAT 1 cut(s) 1517
EcoT38I GRGCYC 1 cut(s) 1697
ErhI CCWWGG 2 cut(s) 996, 2161
FalI AAGNNNNNCTT 8 cut(s) 270, 302, 696, 728, 876, 908, 2158, 2190
FaqI GGGAC 2 cut(s) 349, 1716
FauNDI CATATG 1 cut(s) 667
FbaI TGATCA 2 cut(s) 400, 2044
Fnu4HI GCNGC 8 cut(s) 6, 129, 373, 508, 1108, 1827, 2016, 2126
FokI GGATG 7 cut(s) 212, 602, 1160, 1249, 1258, 1293, 2321
FriOI GRGCYC 1 cut(s) 1697
Fsp4HI GCNGC 8 cut(s) 6, 129, 373, 508, 1108, 1827, 2016, 2126
FspBI CTAG 6 cut(s) 59, 464, 518, 771, 1368, 1854
GluI GCNGC 8 cut(s) 6, 129, 373, 508, 1108, 1827, 2016, 2126
GsaI CCCAGC 1 cut(s) 617
HaeIII GGCC 2 cut(s) 507, 1229
HapII CCGG 2 cut(s) 1310, 1478
HgaI GACGC 1 cut(s) 378
Hin1I GRCGYC 1 cut(s) 370
HincII GTYRAC 1 cut(s) 663
HindII GTYRAC 1 cut(s) 663
HindIII AAGCTT 3 cut(s) 765, 1378, 1664
HpaI GTTAAC 1 cut(s) 663
HpaII CCGG 2 cut(s) 1310, 1478
HphI GGTGA 6 cut(s) 203, 644, 841, 1361, 1576, 1940
Hpy166II GTNNAC 7 cut(s) 663, 761, 1128, 1206, 1550, 1612, 2237
Hpy188I TCNGA 7 cut(s) 460, 897, 1181, 1539, 1894, 1972, 2335
Hpy8I GTNNAC 7 cut(s) 663, 761, 1128, 1206, 1550, 1612, 2237
Hpy99I CGWCG 3 cut(s) 372, 1362, 1910
HpyAV CCTTC 5 cut(s) 794, 927, 1175, 2069, 2160
HpyCH4III ACNGT 7 cut(s) 697, 758, 953, 1117, 1509, 1643, 2186
HpyCH4IV ACGT 3 cut(s) 1024, 1202, 1208
HpyF10VI GCNNNNNNNGC 2 cut(s) 1377, 1709
HpyF3I CTNAG 6 cut(s) 599, 747, 1427, 1473, 1969, 2225
HpySE526I ACGT 3 cut(s) 1024, 1202, 1208
Hsp92I GRCGYC 1 cut(s) 370
KpnI GGTACC 1 cut(s) 990
Ksp22I TGATCA 2 cut(s) 400, 2044
KspAI GTTAAC 1 cut(s) 663
LmnI GCTCC 3 cut(s) 640, 1262, 2297
Lsp1109I GCAGC 5 cut(s) 17, 1094, 1838, 2027, 2112
LweI GCATC 9 cut(s) 80, 315, 343, 1088, 1138, 1729, 1948, 2089, 2100
MabI ACCWGGT 1 cut(s) 654
MaeI CTAG 6 cut(s) 59, 464, 518, 771, 1368, 1854
MaeII ACGT 3 cut(s) 1024, 1202, 1208
MaeIII GTNAC 5 cut(s) 405, 773, 1083, 1856, 2090
MbiI CCGCTC 2 cut(s) 1065, 1424
MboII GAAGA 6 cut(s) 278, 445, 884, 940, 1166, 2241
MfeI CAATTG 1 cut(s) 1605
MflI RGATCY 4 cut(s) 472, 1001, 1568, 2314
MhlI GDGCHC 4 cut(s) 1130, 1534, 1697, 2239
MlyI GAGTC 4 cut(s) 20, 371, 1961, 2164
Mph1103I ATGCAT 1 cut(s) 1517
MroXI GAANNNNTTC 1 cut(s) 1098
MseI TTAA 6 cut(s) 428, 609, 662, 1089, 1140, 1988
MslI CAYNNNNRTG 2 cut(s) 396, 1956
MspA1I CMGCKG 1 cut(s) 71
MspI CCGG 2 cut(s) 1310, 1478
MspR9I CCNGG 3 cut(s) 656, 1311, 1440
MunI CAATTG 1 cut(s) 1605
Mva1269I GAATGC 1 cut(s) 494
MvaI CCWGG 2 cut(s) 656, 1440
MwoI GCNNNNNNNGC 2 cut(s) 1377, 1709
NciI CCSGG 1 cut(s) 1311
NcoI CCATGG 2 cut(s) 996, 2161
NdeI CATATG 1 cut(s) 667
NheI GCTAGC 1 cut(s) 1367
NlaIV GGNNCC 5 cut(s) 642, 988, 1264, 1599, 2137
NmuCI GTSAC 3 cut(s) 405, 1083, 2090
NsiI ATGCAT 1 cut(s) 1517
NspI RCATGY 4 cut(s) 147, 308, 395, 2022
PaeR7I CTCGAG 2 cut(s) 20, 1325
PciI ACATGT 1 cut(s) 391
PctI GAATGC 1 cut(s) 494
PdmI GAANNNNTTC 1 cut(s) 1098
PfoI TCCNGGA 1 cut(s) 1309
PkrI GCNGC 8 cut(s) 7, 130, 374, 509, 1109, 1828, 2017, 2127
Ple19I CGATCG 1 cut(s) 905
PleI GAGTC 4 cut(s) 20, 370, 1961, 2164
PpsI GAGTC 4 cut(s) 20, 370, 1961, 2164
PscI ACATGT 1 cut(s) 391
Psp6I CCWGG 2 cut(s) 654, 1438
PspFI CCCAGC 1 cut(s) 613
PspGI CCWGG 2 cut(s) 654, 1438
PspN4I GGNNCC 5 cut(s) 642, 988, 1264, 1599, 2137
PspPI GGNCC 1 cut(s) 227
PspXI VCTCGAGB 1 cut(s) 20
PstI CTGCAG 2 cut(s) 793, 2195
PsuI RGATCY 4 cut(s) 472, 1001, 1568, 2314
PvuI CGATCG 1 cut(s) 905
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 6 cut(s) 945, 988, 1205, 1551, 1815, 2274
RsaNI GTAC 6 cut(s) 944, 987, 1204, 1550, 1814, 2273
RseI CAYNNNNRTG 2 cut(s) 396, 1956
SaqAI TTAA 6 cut(s) 428, 609, 662, 1089, 1140, 1988
SatI GCNGC 8 cut(s) 6, 129, 373, 508, 1108, 1827, 2016, 2126
Sau96I GGNCC 1 cut(s) 227
SchI GAGTC 4 cut(s) 20, 371, 1961, 2164
ScrFI CCNGG 3 cut(s) 656, 1311, 1440
SduI GDGCHC 4 cut(s) 1130, 1534, 1697, 2239
SexAI ACCWGGT 1 cut(s) 654
SfaNI GCATC 9 cut(s) 80, 315, 343, 1088, 1138, 1729, 1948, 2089, 2100
SfcI CTRYAG 2 cut(s) 789, 2191
Sfr274I CTCGAG 2 cut(s) 20, 1325
SinI GGWCC 1 cut(s) 227
SlaI CTCGAG 2 cut(s) 20, 1325
SmiMI CAYNNNNRTG 2 cut(s) 396, 1956
SmlI CTYRAG 3 cut(s) 20, 1005, 1325
SmoI CTYRAG 3 cut(s) 20, 1005, 1325
SpeI ACTAGT 1 cut(s) 1853
SsiI CCGC 6 cut(s) 128, 373, 508, 1044, 1063, 1422
SspI AATATT 1 cut(s) 538
SspMI CTAG 6 cut(s) 59, 464, 518, 771, 1368, 1854
StyD4I CCNGG 3 cut(s) 654, 1309, 1438
StyI CCWWGG 2 cut(s) 996, 2161
TaaI ACNGT 7 cut(s) 697, 758, 953, 1117, 1509, 1643, 2186
TaiI ACGT 3 cut(s) 1027, 1205, 1211
TaqI TCGA 8 cut(s) 21, 259, 498, 1168, 1326, 1360, 1908, 2115
TatI WGTACW 1 cut(s) 1549
TauI GCSGC 3 cut(s) 131, 375, 510
Tru1I TTAA 6 cut(s) 428, 609, 662, 1089, 1140, 1988
Tru9I TTAA 6 cut(s) 428, 609, 662, 1089, 1140, 1988
TscAI CASTG 3 cut(s) 237, 2189, 2200
TseFI GTSAC 3 cut(s) 405, 1083, 2090
TseI GCWGC 5 cut(s) 5, 1107, 1826, 2015, 2125
Tsp45I GTSAC 3 cut(s) 405, 1083, 2090
TspGWI ACGGA 3 cut(s) 1402, 2271, 2321
TspRI CASTG 3 cut(s) 237, 2189, 2200
VneI GTGCAC 2 cut(s) 1126, 2235
VpaK11BI GGWCC 1 cut(s) 227
XapI RAATTY 7 cut(s) 242, 523, 560, 731, 910, 1282, 1758
XbaI TCTAGA 1 cut(s) 58
XceI RCATGY 4 cut(s) 147, 308, 395, 2022
XhoI CTCGAG 2 cut(s) 20, 1325
XmnI GAANNNNTTC 1 cut(s) 1098
XspI CTAG 6 cut(s) 59, 464, 518, 771, 1368, 1854
Zsp2I ATGCAT 1 cut(s) 1517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.