MD16G1006300.v1.1

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0007229
Physical Location & Seq
Forward (+)
488818 .. 496030
7213 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1006300.v1.1.491

Sequence Viewer

Length: 2373 bp
ATGGCGGCCTTCATCAAGCTCGAGGATTCCCCAATGTTTCAGAAGCAGATGTATTCTATGGAACAAACAGCTGATGAGTTGAAAGATCGCTGCCAAAAATTATGCAAAGGGTCTAAGAAGCTTATGGGGGCTCTGGAAGAAGCATGCAATGGAGACACCATTTTTTCAGAATCTTTGGAAGCATTTGGTGGTGGACTGGACGATCCTATTAGTGTATCCATTGGAGGTCCAGTATTATCCAAATTCGTTACTGCATTTCAAGAGCTTGCCACTTATAAGGAGCTTCTGCGCTCCCAAGTAGAGCACGTTTTAGTTGATCGGTTGATGCACTTTATGACTGTTGATCTGCAAGATGCAAAGGAGTCCCGACGTCAATTCGACAAAGCTATACATGGTTATGATCAGGCACGGGAAAAGTTTGTGTCCTTAAAGAAGAATACACGGGAAGACATTGTTGCAGAACTAGAGGAGGATCTACAAAATTCAAAATCAACATTTGAGAAAAGCCGCTTCAACCTAGTAAACTCACTAATGAATATTGAAGCAAAAAAGAAGTATGAGTTTTTGGAGTCTATTAGTGCAATCATGGATGCGCATCTGAGATACTTTAAGCTGGGATACGAGTTATTCAGCCAAATGGAACCATATATTCACCAGGTGTTAACGTACGCACAACAATCTAAAGAACAGGCCACTGTTGAACAAGATAAACTTCACAAAAGGATTCAGGAATATAGGACACAAGCTGAGCTGGACAGTATACGAGCTTCCAGTAATATTGAGCCTCCTGCAGTGGCTGATGGAAATCGCGCCTTTGGTTTAGCTTCTCACAAAAACATAGAAGCAATCATGCAGTCCAGTGCAAATGGGAAGGTCCAGACGATCAAGCAAGGATATCTAGTGAAACGATCTTCAAGTTTGAGGGGAGATTGGAAGAGGAGGTTTTTCGTTCTGAACAGTCATGGGGCTTTATTTTATTACAGAATTAAGGGTACCAAACCCATGGGCGGCTCCCAGTCACATCATTTCACTCGCTCACCGGAACATCACAGTGGTGTGTTTAGCAGATTTCGTTCAAGACATAGGGTAGCGTCACTTAATGAAAACATTCTGGGCTGCCGTACTGTTGATCTGCGTACCTCAACAATAAAGATGGACGCAGAGGATACAGATCTTCGACTTTGCTTTAGAATAATATCCCCATTGAAAACTTATACATTGCAGGCTGAAAATGAAGCTGATAGGATGGATTGGACAAACAAAATAGCAGGAGTTATTGCATCTCTTTTGAATTTTCAGCTTCTTGACCAGCCACATCCTGGAACTGTGAATCACGAGAGTAGTAAATCTTCTCTTGGTACTTATGATATTCGTAGAGTGAAAAGCACTGAAAGTTTAGGAAATACCAGAAAATTCAACAGAGTAGATTCTGTTTCTAGTATTCTCAGGAGAATTCCTGGAAATGATCTTTGTGCGGAGTGCGGTGCTCCTGAGCCTGACTGGGCATCTCTTAATCTTGGCGTACTGATGTGCATTGAATGTTCTGGTGTTCACCGGAATCTTGGAGTACACATTTCAAAGGTGAGATCTCTAACATTAGATGTCAAGGTTTGGGAACCTATACTTGTGGACTTATTTCGCAACTTGGGTAATGCATATTGTAATTCTGTATGGGAGGGAACGCTTCTACTTGAGAATGAAAAACTGGATGGTTCGAAGGACATGAGGGCTCCAGTTTTAAAACCAGGCCCCAAGGATGCAGCTCAGCATAAGGAAATATACATTCAAGCAAAGTATGTGGAGAAAGTGATGGTTATTAGAGATGTAGCTGCATCGGGCATGCCTGTTCTTGCAGCAGATATATGGGAAGCAGTCAAGGCTGGTAATTTACAAGAAGTCTACCGCATTATCGTGATGTCAGATGTGAATATTGTCGACACCGCCTTTGAGAATGTGGTTACTCATGATTTATATCACCATGCTGAAGCAATACAGTCGGAGTCAGATTTTAACACGATGGAGATGAAACTGTATGATCCAGCAGCATGTGATAGAATCAAGGAGTCCAATGAGCCTGGGAACTGTCTTCAAGGTTGTTCATTATTGCATCTGGCTTGTCACTCTGGCAATCCGATGATGGTTGAATTGTTGCTGCAGTTTGGTGCCAACATGAATTTGCGGGATTTCCATGGAAGGACTCCTTTGCACCACTGCATCGCCAGCGGGAACAACTCATTGGCAAAGTGGCTACTTAGGAGGGGTGCACGCCCGTCAATTCAAGATGGGGGAGGGCAAAGTGCATTAGAAAGAGCGATGGAGCTGGGAGCAATTACAGACGAGGAGTTGTTCATACTACTTTCAGAACACGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_5 ArfGap BAR_3 PH

Protein Analysis

791

Amino Acids

88.96

Weight (kDa)

6.4

Isoelectric Point (pI)

47.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BAR_3 PF16746 7 - 238 2.8e-37 BAR domain of APPL family
BAR PF03114 19 - 217 3.7e-11 BAR domain
PH PF00169 295 - 425 2.9e-10 PH domain
ArfGap PF01412 480 - 605 7.2e-31 Putative GTPase activating protein for Arf
Ank_2 PF12796 689 - 761 6.2e-13 Ankyrin repeats (3 copies)
Ank_5 PF13857 694 - 737 3.4e-06 Ankyrin repeats (many copies)
Ank PF00023 698 - 728 3.8e-07 Ankyrin repeat
Ank_4 PF13637 702 - 751 1.2e-08 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 276
AatII GACGTC 1 cut(s) 373
Acc16I TGCGCA 1 cut(s) 594
Acc65I GGTACC 1 cut(s) 992
AccB1I GGYRCC 2 cut(s) 992, 2162
AccB7I CCANNNNNTGG 1 cut(s) 1319
AccI GTMKAC 3 cut(s) 760, 1899, 1935
AccII CGCG 1 cut(s) 810
AciI CCGC 9 cut(s) 5, 508, 1008, 1475, 1482, 1903, 1941, 2179, 2223
AclWI GGATC 3 cut(s) 197, 480, 2030
AcsI RAATTY 6 cut(s) 242, 481, 1291, 1412, 1452, 2173
AcuI CTGAAG 1 cut(s) 2004
AcyI GRCGYC 1 cut(s) 370
AdeI CACNNNGTG 1 cut(s) 658
AfaI GTAC 7 cut(s) 668, 994, 1123, 1138, 1360, 1524, 1569
AfiI CCNNNNNNNGG 2 cut(s) 1007, 1319
AjnI CCWGG 5 cut(s) 654, 1318, 1456, 1744, 2074
AjuI GAANNNNNNNTTGG 2 cut(s) 2219, 2251
AleI CACNNNNGTG 1 cut(s) 1053
Alw21I GWGCWC 3 cut(s) 306, 1489, 2266
Alw26I GTCTC 1 cut(s) 147
Alw44I GTGCAC 1 cut(s) 2262
AlwI GGATC 3 cut(s) 197, 480, 2030
AlwNI CAGNNNCTG 1 cut(s) 797
Ama87I CYCGRG 1 cut(s) 20
AoxI GGCC 3 cut(s) 6, 690, 1747
ApaLI GTGCAC 1 cut(s) 2262
ApeKI GCWGC 7 cut(s) 90, 1116, 1760, 1829, 1853, 2042, 2152
ApoI RAATTY 6 cut(s) 242, 481, 1291, 1412, 1452, 2173
ArsI GACNNNNNNTTYG 2 cut(s) 1928, 1960
Asp700I GAANNNNTTC 1 cut(s) 1107
Asp718I GGTACC 1 cut(s) 992
AspLEI GCGC 3 cut(s) 291, 595, 812
AspS9I GGNCC 3 cut(s) 227, 874, 1748
AsuHPI GGTGA 5 cut(s) 644, 1029, 1544, 1594, 1967
AsuII TTCGAA 1 cut(s) 1715
AvaI CYCGRG 1 cut(s) 20
AvaII GGWCC 2 cut(s) 227, 874
BaeGI GKGCMC 1 cut(s) 2266
BanI GGYRCC 2 cut(s) 992, 2162
BanII GRGCYC 2 cut(s) 133, 1732
BarI GAAGNNNNNNTAC 4 cut(s) 751, 783, 1333, 1365
BauI CACGAG 2 cut(s) 1334, 2366
BbsI GAAGAC 2 cut(s) 453, 2078
Bbv12I GWGCWC 3 cut(s) 306, 1489, 2266
BbvI GCAGC 7 cut(s) 77, 1103, 1772, 1816, 1865, 2054, 2139
BccI CCATC 9 cut(s) 794, 1147, 1240, 1703, 1804, 2011, 2131, 2276, 2308
BceAI ACGGC 1 cut(s) 1104
BcgI CGANNNNNNTGC 2 cut(s) 1977, 2011
BciT130I CCWGG 5 cut(s) 656, 1320, 1458, 1746, 2076
BciVI GTATCC 3 cut(s) 226, 611, 1159
BclI TGATCA 1 cut(s) 400
BcoDI GTCTC 1 cut(s) 147
BfaI CTAG 4 cut(s) 464, 518, 899, 1437
BfmI CTRYAG 2 cut(s) 789, 2153
BfuI GTATCC 3 cut(s) 226, 611, 1159
BglII AGATCT 2 cut(s) 1171, 1586
BlpI GCTNAGC 2 cut(s) 747, 1764
Bme1390I CCNGG 5 cut(s) 656, 1320, 1458, 1746, 2076
Bme18I GGWCC 2 cut(s) 227, 874
BmeT110I CYCGRG 1 cut(s) 20
BmgT120I GGNCC 3 cut(s) 227, 874, 1748
BmiI GGNNCC 7 cut(s) 642, 994, 1012, 1617, 1731, 1750, 2164
BmrFI CCNGG 5 cut(s) 656, 1320, 1458, 1746, 2076
BmrI ACTGGG 2 cut(s) 1009, 1510
BmuI ACTGGG 2 cut(s) 1009, 1510
BpiI GAAGAC 2 cut(s) 453, 2078
BpmI CTGGAG 1 cut(s) 1716
Bpu10I CCTNAGC 1 cut(s) 1491
Bpu1102I GCTNAGC 2 cut(s) 747, 1764
Bpu14I TTCGAA 1 cut(s) 1715
BpuEI CTTGAG 1 cut(s) 1712
BsaBI GATNNNNATC 4 cut(s) 594, 804, 1170, 1971
BsaHI GRCGYC 1 cut(s) 370
BsaJI CCNNGG 4 cut(s) 1002, 1752, 2075, 2188
BsaWI WCCGGW 2 cut(s) 1039, 1554
BsaXI ACNNNNNCTCC 2 cut(s) 216, 246
Bsc4I CCNNNNNNNGG 2 cut(s) 1007, 1319
Bse1I ACTGG 8 cut(s) 201, 230, 771, 858, 1015, 1505, 1710, 1733
Bse3DI GCAATG 2 cut(s) 154, 1217
Bse8I GATNNNNATC 4 cut(s) 594, 804, 1170, 1971
BseBI CCWGG 5 cut(s) 656, 1320, 1458, 1746, 2076
BseDI CCNNGG 4 cut(s) 1002, 1752, 2075, 2188
BseGI GGATG 5 cut(s) 595, 1251, 1315, 1714, 1762
BseJI GATNNNNATC 4 cut(s) 594, 804, 1170, 1971
BseLI CCNNNNNNNGG 2 cut(s) 1007, 1319
BseMI GCAATG 2 cut(s) 154, 1217
BseMII CTCAG 5 cut(s) 590, 738, 1459, 1482, 1778
BseNI ACTGG 8 cut(s) 201, 230, 771, 858, 1015, 1505, 1710, 1733
BseRI GAGGAG 3 cut(s) 482, 952, 2354
BseSI GKGCMC 1 cut(s) 2266
BseXI GCAGC 7 cut(s) 77, 1103, 1772, 1816, 1865, 2054, 2139
BseYI CCCAGC 2 cut(s) 613, 2320
Bsh1236I CGCG 1 cut(s) 810
BshFI GGCC 3 cut(s) 8, 692, 1749
BshNI GGYRCC 2 cut(s) 992, 2162
BsiHKAI GWGCWC 3 cut(s) 306, 1489, 2266
BsiHKCI CYCGRG 1 cut(s) 20
BsiSI CCGG 2 cut(s) 1040, 1555
BsiWI CGTACG 1 cut(s) 666
BslFI GGGAC 1 cut(s) 349
BslI CCNNNNNNNGG 2 cut(s) 1007, 1319
BsmAI GTCTC 1 cut(s) 147
BsmFI GGGAC 1 cut(s) 349
BsnI GGCC 3 cut(s) 8, 692, 1749
BsoBI CYCGRG 1 cut(s) 20
Bsp119I TTCGAA 1 cut(s) 1715
Bsp1286I GDGCHC 5 cut(s) 133, 306, 1489, 1732, 2266
Bsp1720I GCTNAGC 2 cut(s) 747, 1764
Bsp19I CCATGG 2 cut(s) 1002, 2188
BspACI CCGC 9 cut(s) 5, 508, 1008, 1475, 1482, 1903, 1941, 2179, 2223
BspANI GGCC 3 cut(s) 8, 692, 1749
BspCNI CTCAG 5 cut(s) 591, 739, 1458, 1483, 1777
BspFNI CGCG 1 cut(s) 810
BspHI TCATGA 1 cut(s) 1963
BspLI GGNNCC 7 cut(s) 642, 994, 1012, 1617, 1731, 1750, 2164
BspMAI CTGCAG 2 cut(s) 793, 2157
BspPI GGATC 3 cut(s) 197, 480, 2030
BspT104I TTCGAA 1 cut(s) 1715
BspT107I GGYRCC 2 cut(s) 992, 2162
BsrDI GCAATG 2 cut(s) 154, 1217
BsrI ACTGG 8 cut(s) 201, 230, 771, 858, 1015, 1505, 1710, 1733
BssECI CCNNGG 4 cut(s) 1002, 1752, 2075, 2188
BssNAI GTATAC 1 cut(s) 761
BssNI GRCGYC 1 cut(s) 370
BssSI CACGAG 2 cut(s) 1334, 2366
BssT1I CCWWGG 3 cut(s) 1002, 1752, 2188
Bst1107I GTATAC 1 cut(s) 761
Bst2BI CACGAG 2 cut(s) 1334, 2366
Bst2UI CCWGG 5 cut(s) 656, 1320, 1458, 1746, 2076
Bst6I CTCTTC 1 cut(s) 929
BstACI GRCGYC 1 cut(s) 370
BstBI TTCGAA 1 cut(s) 1715
BstC8I GCNNGC 6 cut(s) 145, 267, 1224, 1841, 2221, 2266
BstDEI CTNAG 7 cut(s) 114, 599, 747, 1445, 1491, 1764, 2252
BstDSI CCRYGG 2 cut(s) 1002, 2188
BstF5I GGATG 5 cut(s) 595, 1251, 1315, 1714, 1762
BstFNI CGCG 1 cut(s) 810
BstHHI GCGC 3 cut(s) 291, 595, 812
BstMAI GTCTC 1 cut(s) 147
BstMWI GCNNNNNNNGC 2 cut(s) 1877, 2220
BstNI CCWGG 5 cut(s) 656, 1320, 1458, 1746, 2076
BstNSI RCATGY 3 cut(s) 147, 1843, 2049
BstSCI CCNGG 5 cut(s) 654, 1318, 1456, 1744, 2074
BstSFI CTRYAG 2 cut(s) 789, 2153
BstSLI GKGCMC 1 cut(s) 2266
BstUI CGCG 1 cut(s) 810
BstV1I GCAGC 7 cut(s) 77, 1103, 1772, 1816, 1865, 2054, 2139
BstV2I GAAGAC 2 cut(s) 453, 2078
BstX2I RGATCY 3 cut(s) 472, 1171, 1586
BstXI CCANNNNNNTGG 1 cut(s) 1003
BstYI RGATCY 3 cut(s) 472, 1171, 1586
BstZ17I GTATAC 1 cut(s) 761
BsuI GTATCC 3 cut(s) 226, 611, 1159
BsuRI GGCC 3 cut(s) 8, 692, 1749
BtgI CCRYGG 2 cut(s) 1002, 2188
BtgZI GCGATG 2 cut(s) 2200, 2327
BtsCI GGATG 5 cut(s) 595, 1251, 1315, 1714, 1762
BtsI GCAGTG 2 cut(s) 798, 2209
BtsIMutI CAGTG 6 cut(s) 693, 798, 865, 1057, 1386, 2209
Cac8I GCNNGC 6 cut(s) 145, 267, 1224, 1841, 2221, 2266
CaiI CAGNNNCTG 1 cut(s) 797
CciI TCATGA 1 cut(s) 1963
CfoI GCGC 3 cut(s) 291, 595, 812
Cfr13I GGNCC 3 cut(s) 227, 874, 1748
CseI GACGC 2 cut(s) 1080, 1166
CsiI ACCWGGT 1 cut(s) 654
Csp6I GTAC 7 cut(s) 667, 993, 1122, 1137, 1359, 1523, 1568
CspCI CAANNNNNGTGG 2 cut(s) 1779, 1814
CviQI GTAC 7 cut(s) 667, 993, 1122, 1137, 1359, 1523, 1568
DdeI CTNAG 7 cut(s) 114, 599, 747, 1445, 1491, 1764, 2252
DraI TTTAAA 1 cut(s) 1740
DraIII CACNNNGTG 1 cut(s) 658
Eam1104I CTCTTC 1 cut(s) 929
EarI CTCTTC 1 cut(s) 929
Eco130I CCWWGG 3 cut(s) 1002, 1752, 2188
Eco24I GRGCYC 2 cut(s) 133, 1732
Eco32I GATATC 1 cut(s) 896
Eco47I GGWCC 2 cut(s) 227, 874
Eco57I CTGAAG 1 cut(s) 2004
Eco88I CYCGRG 1 cut(s) 20
EcoO109I RGGNCCY 1 cut(s) 1748
EcoRI GAATTC 1 cut(s) 1452
EcoRII CCWGG 5 cut(s) 654, 1318, 1456, 1744, 2074
EcoRV GATATC 1 cut(s) 896
EcoT14I CCWWGG 3 cut(s) 1002, 1752, 2188
EcoT22I ATGCAT 1 cut(s) 1657
EcoT38I GRGCYC 2 cut(s) 133, 1732
ErhI CCWWGG 3 cut(s) 1002, 1752, 2188
FalI AAGNNNNNCTT 4 cut(s) 696, 728, 2185, 2217
FaqI GGGAC 1 cut(s) 349
FauI CCCGC 2 cut(s) 2172, 2216
FbaI TGATCA 1 cut(s) 400
FblI GTMKAC 3 cut(s) 760, 1899, 1935
FokI GGATG 5 cut(s) 602, 1258, 1302, 1721, 1769
FriOI GRGCYC 2 cut(s) 133, 1732
FspAI RTGCGCAY 1 cut(s) 594
FspBI CTAG 4 cut(s) 464, 518, 899, 1437
FspI TGCGCA 1 cut(s) 594
GlaI GCGC 3 cut(s) 290, 594, 811
GsaI CCCAGC 2 cut(s) 617, 2324
GsuI CTGGAG 1 cut(s) 1716
HaeIII GGCC 3 cut(s) 8, 692, 1749
HapII CCGG 2 cut(s) 1040, 1555
HgaI GACGC 2 cut(s) 1080, 1166
HhaI GCGC 3 cut(s) 291, 595, 812
Hin1I GRCGYC 1 cut(s) 370
Hin6I GCGC 3 cut(s) 289, 593, 810
HinP1I GCGC 3 cut(s) 289, 593, 810
HincII GTYRAC 2 cut(s) 663, 1936
HindII GTYRAC 2 cut(s) 663, 1936
HindIII AAGCTT 1 cut(s) 119
HpaI GTTAAC 1 cut(s) 663
HpaII CCGG 2 cut(s) 1040, 1555
HphI GGTGA 5 cut(s) 644, 1029, 1544, 1594, 1967
Hpy188I TCNGA 9 cut(s) 42, 169, 600, 954, 1921, 1999, 2005, 2133, 2362
Hpy99I CGWCG 1 cut(s) 372
HpyAV CCTTC 4 cut(s) 19, 865, 1711, 2187
HpyCH4IV ACGT 3 cut(s) 306, 370, 665
HpyF10VI GCNNNNNNNGC 2 cut(s) 1877, 2220
HpyF3I CTNAG 7 cut(s) 114, 599, 747, 1445, 1491, 1764, 2252
HpySE526I ACGT 3 cut(s) 306, 370, 665
Hsp92I GRCGYC 1 cut(s) 370
HspAI GCGC 3 cut(s) 289, 593, 810
KpnI GGTACC 1 cut(s) 996
Ksp22I TGATCA 1 cut(s) 400
KspAI GTTAAC 1 cut(s) 663
LmnI GCTCC 7 cut(s) 280, 296, 1016, 1492, 1735, 2317, 2324
Lsp1109I GCAGC 7 cut(s) 77, 1103, 1772, 1816, 1865, 2054, 2139
MabI ACCWGGT 1 cut(s) 654
MaeI CTAG 4 cut(s) 464, 518, 899, 1437
MaeII ACGT 3 cut(s) 306, 370, 665
MaeIII GTNAC 5 cut(s) 247, 1017, 1092, 1957, 2117
MboII GAAGA 8 cut(s) 149, 445, 458, 903, 946, 1166, 1341, 2078
MflI RGATCY 3 cut(s) 472, 1171, 1586
MhlI GDGCHC 5 cut(s) 133, 306, 1489, 1732, 2266
MlyI GAGTC 5 cut(s) 371, 578, 2009, 2072, 2191
MmeI TCCRAC 1 cut(s) 1977
Mph1103I ATGCAT 1 cut(s) 1657
MroXI GAANNNNTTC 1 cut(s) 1107
MseI TTAA 8 cut(s) 428, 609, 662, 987, 1098, 1512, 1739, 2010
MslI CAYNNNNRTG 4 cut(s) 396, 1050, 1053, 1910
MspA1I CMGCKG 2 cut(s) 71, 2223
MspI CCGG 2 cut(s) 1040, 1555
MspR9I CCNGG 5 cut(s) 656, 1320, 1458, 1746, 2076
MvaI CCWGG 5 cut(s) 656, 1320, 1458, 1746, 2076
MvnI CGCG 1 cut(s) 810
MwoI GCNNNNNNNGC 2 cut(s) 1877, 2220
NcoI CCATGG 2 cut(s) 1002, 2188
NlaIV GGNNCC 7 cut(s) 642, 994, 1012, 1617, 1731, 1750, 2164
NmuCI GTSAC 3 cut(s) 1017, 1092, 2117
NsbI TGCGCA 1 cut(s) 594
NsiI ATGCAT 1 cut(s) 1657
NspI RCATGY 3 cut(s) 147, 1843, 2049
NspV TTCGAA 1 cut(s) 1715
OliI CACNNNNGTG 1 cut(s) 1053
PaeI GCATGC 2 cut(s) 147, 1843
PaeR7I CTCGAG 1 cut(s) 20
PagI TCATGA 1 cut(s) 1963
PdmI GAANNNNTTC 1 cut(s) 1107
PfeI GAWTC 7 cut(s) 26, 170, 724, 1330, 1427, 1558, 2055
Pfl23II CGTACG 1 cut(s) 666
PflMI CCANNNNNTGG 1 cut(s) 1319
PfoI TCCNGGA 2 cut(s) 1318, 1456
PleI GAGTC 5 cut(s) 370, 577, 2008, 2071, 2191
PpsI GAGTC 5 cut(s) 370, 577, 2008, 2071, 2191
PsiI TTATAA 1 cut(s) 276
Psp6I CCWGG 5 cut(s) 654, 1318, 1456, 1744, 2074
PspFI CCCAGC 2 cut(s) 613, 2320
PspGI CCWGG 5 cut(s) 654, 1318, 1456, 1744, 2074
PspLI CGTACG 1 cut(s) 666
PspN4I GGNNCC 7 cut(s) 642, 994, 1012, 1617, 1731, 1750, 2164
PspPI GGNCC 3 cut(s) 227, 874, 1748
PspXI VCTCGAGB 1 cut(s) 20
PstI CTGCAG 2 cut(s) 793, 2157
PstNI CAGNNNCTG 1 cut(s) 797
PsuI RGATCY 3 cut(s) 472, 1171, 1586
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 7 cut(s) 668, 994, 1123, 1138, 1360, 1524, 1569
RsaNI GTAC 7 cut(s) 667, 993, 1122, 1137, 1359, 1523, 1568
RseI CAYNNNNRTG 4 cut(s) 396, 1050, 1053, 1910
SalI GTCGAC 1 cut(s) 1934
SaqAI TTAA 8 cut(s) 428, 609, 662, 987, 1098, 1512, 1739, 2010
Sau96I GGNCC 3 cut(s) 227, 874, 1748
SchI GAGTC 5 cut(s) 371, 578, 2009, 2072, 2191
ScrFI CCNGG 5 cut(s) 656, 1320, 1458, 1746, 2076
SduI GDGCHC 5 cut(s) 133, 306, 1489, 1732, 2266
SexAI ACCWGGT 1 cut(s) 654
SfcI CTRYAG 2 cut(s) 789, 2153
Sfr274I CTCGAG 1 cut(s) 20
SfuI TTCGAA 1 cut(s) 1715
SinI GGWCC 2 cut(s) 227, 874
SlaI CTCGAG 1 cut(s) 20
SmiMI CAYNNNNRTG 4 cut(s) 396, 1050, 1053, 1910
SmlI CTYRAG 2 cut(s) 20, 1691
SmoI CTYRAG 2 cut(s) 20, 1691
SphI GCATGC 2 cut(s) 147, 1843
SsiI CCGC 9 cut(s) 5, 508, 1008, 1475, 1482, 1903, 1941, 2179, 2223
SspI AATATT 3 cut(s) 538, 778, 1930
SspMI CTAG 4 cut(s) 464, 518, 899, 1437
StyD4I CCNGG 5 cut(s) 654, 1318, 1456, 1744, 2074
StyI CCWWGG 3 cut(s) 1002, 1752, 2188
TaiI ACGT 3 cut(s) 309, 373, 668
TaqI TCGA 5 cut(s) 21, 378, 1177, 1715, 1935
TatI WGTACW 1 cut(s) 1567
TauI GCSGC 3 cut(s) 8, 510, 1011
TfiI GAWTC 7 cut(s) 26, 170, 724, 1330, 1427, 1558, 2055
Tru1I TTAA 8 cut(s) 428, 609, 662, 987, 1098, 1512, 1739, 2010
Tru9I TTAA 8 cut(s) 428, 609, 662, 987, 1098, 1512, 1739, 2010
TscAI CASTG 6 cut(s) 700, 798, 865, 1057, 1393, 2216
TseFI GTSAC 3 cut(s) 1017, 1092, 2117
TseI GCWGC 7 cut(s) 90, 1116, 1760, 1829, 1853, 2042, 2152
Tsp45I GTSAC 3 cut(s) 1017, 1092, 2117
TspDTI ATGAA 8 cut(s) 548, 1116, 1248, 1713, 2039, 2088, 2186, 2338
TspRI CASTG 6 cut(s) 700, 798, 865, 1057, 1393, 2216
Van91I CCANNNNNTGG 1 cut(s) 1319
VneI GTGCAC 1 cut(s) 2262
VpaK11BI GGWCC 2 cut(s) 227, 874
XapI RAATTY 6 cut(s) 242, 481, 1291, 1412, 1452, 2173
XceI RCATGY 3 cut(s) 147, 1843, 2049
XcmI CCANNNNNNNNNTGG 1 cut(s) 1316
XhoI CTCGAG 1 cut(s) 20
XmiI GTMKAC 3 cut(s) 760, 1899, 1935
XmnI GAANNNNTTC 1 cut(s) 1107
XspI CTAG 4 cut(s) 464, 518, 899, 1437
ZraI GACGTC 1 cut(s) 371
Zsp2I ATGCAT 1 cut(s) 1657
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.