AT1G65080

60Kd inner membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001721
Physical Location & Seq
Forward (+)
24176563 .. 24180871
4309 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G65080.1

Sequence Viewer

Length: 1578 bp
ATGGCAGTGTGGAGGAGTTGTTTACGCGCCTCCTCCTCTCGTCACCGTTTATCTTCTCTCTTCTTCTCCCACCACCTCAAACCCTCCTCCTTCATCTCCCATTCTCCTCCTCTCTTCTCTCCCTCGCCGTCACCATCTCCGACGTGTTTCTCTCCTCTCCACCGATTACTCTCTTCTGGACCAGAATTCCCAATTGGGTCTCAAGAAATCATCCCTACCGATGATTCTTCTTTACCTGTTCTCGCCGTCGTTGATTTCCTCGAAGGCTTTCATGAATTCACAGGATTGCCTTGGTGGATGATTATTGCATCTTCTACTGTTGCTGTTAGATTAGCTTTATTGCCTTTACTCATACTTCAACTCAAGAAATTGAAGACCATTTCAGAGCTGCTACCGAAGTTGCCTATGCCGATCCCAGAGACTCCAACGTTGAAAGGATCTATTGACCAGTTTTCTCATTTTCTTAAAGAGAGTCGAGCAATTGGGTGCCCCTCCTTTTTGTGGTTCTTCCCATACTTATCTGTCCAGCTCCCATGCTTTTTCTTGTTGATGGCTAGCATCCGGAAGATGTCACTAGATGGTCACCCTGGCTTTGATTCTGGTGGTGTCTTATGGTTCCAGAACTTGAGTGATCTTCCTGGTGGCTCTTTTGGACCAGTCTTCCCAATTTTAATTGCTACCTTCCATTACATTAATATACAGATTTCTTTTGATACATCCACAATTCGTCAAACTACCGGCTTGACTGGGCTGCTCATGAGATATTACAAGTTATATCTGGAGATCTTAAGCGTTCCTCTGTTTTTTGTTGGCTATGCTATTCCACAGGGAAGTCTTGTCTACTGGGTAACCAACAGCTCAGTAAATATCTTTCAGCAATTATCTCTCAAGCACCCTACTGTAGGAGCAAAACTTGGTTTACTGAGTCAGGGGGCCACTCCAGGCATGGGACATTCCATGGAGATCAGCGAATCTGTGATCAAGTATGTGGACTCTGACTCAAAAGAACAGACCCTTTCTCTACAGACTCTAACACCAGAAGAACTTCTTTCTCTTTCAGTTCAGGTCTTGTCCAAAGGTGATAAAGAAACATCCATTCAGTTGCTTCGATTAGCTCTTGAAAAGGATCCTGGATATGTAAGAGGTTTGGTTCTCATGGGGCAGGCGTTGTTACAGAAGACGCAGTTGTCAGAAGCTACTGAATATTTAGAGCTTGCTATCTCCAAGCTCCTTGATGAGGATCCATCTGATGCTGAGGATGTTGAACTTTTAATGCTTGCATCTCAGTGGGCTGGTGCTGCATACGTACAACAGGGAAAACTGAAAAGCGGAATCATACACTTGGAAAGAGTAGCGAAACTGAGAGAACCTGGTGATCCTAAAAGCAAAGAGCATTACTTTGAGGCATTGCTGCTTCTTTCAAGCGCTTTGTACAAAGAAGGGCAGAGTGATGAAGCTGCAAAGATTTTACGAGTAGTAGTGGATCACAATCCGGCGTACAAGCCTCTGCTGGAACAGTGTGAAGATGAGAATGAGCTAGTTAGCGATCTTGTCAGTCGCAGAAAGGATCATTTCTGA

Protein Analysis

525

Amino Acids

58.29

Weight (kDa)

5.94

Isoelectric Point (pI)

47.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
60KD_IMP PF02096 98 - 296 7.8e-10 60Kd inner membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1186
AccB1I GGYRCC 1 cut(s) 486
AccI GTMKAC 1 cut(s) 840
AccII CGCG 1 cut(s) 27
AccIII TCCGGA 1 cut(s) 561
AciI CCGC 1 cut(s) 1329
AclI AACGTT 1 cut(s) 428
AclWI GGATC 9 cut(s) 406, 445, 1121, 1134, 1235, 1248, 1370, 1491, 1575
AcsI RAATTY 2 cut(s) 185, 275
AfaI GTAC 3 cut(s) 1308, 1433, 1499
AfeI AGCGCT 1 cut(s) 1426
AfiI CCNNNNNNNGG 4 cut(s) 501, 902, 947, 1237
AflII CTTAAG 1 cut(s) 787
AflIII ACRYGT 1 cut(s) 143
AgsI TTSAA 6 cut(s) 359, 373, 433, 1121, 1265, 1422
AjiI CACGTC 1 cut(s) 144
AjnI CCWGG 5 cut(s) 586, 637, 940, 1129, 1369
AjuI GAANNNNNNNTTGG 2 cut(s) 177, 209
Alw26I GTCTC 2 cut(s) 204, 413
AlwI GGATC 9 cut(s) 406, 445, 1121, 1134, 1235, 1248, 1370, 1491, 1575
Aor13HI TCCGGA 1 cut(s) 561
Aor51HI AGCGCT 1 cut(s) 1426
AoxI GGCC 1 cut(s) 933
ApeKI GCWGC 5 cut(s) 388, 751, 1298, 1411, 1457
ApoI RAATTY 2 cut(s) 185, 275
AseI ATTAAT 1 cut(s) 693
Asp700I GAANNNNTTC 2 cut(s) 267, 1044
AspLEI GCGC 2 cut(s) 29, 1427
AspS9I GGNCC 3 cut(s) 179, 653, 933
AsuHPI GGTGA 5 cut(s) 35, 123, 575, 1091, 1385
AsuNHI GCTAGC 1 cut(s) 554
AvaII GGWCC 2 cut(s) 179, 653
BaeGI GKGCMC 1 cut(s) 491
BamHI GGATCC 2 cut(s) 1126, 1240
BanI GGYRCC 1 cut(s) 486
BbsI GAAGAC 3 cut(s) 380, 652, 1184
BbvCI CCTCAGC 1 cut(s) 1254
BbvI GCAGC 5 cut(s) 375, 738, 1285, 1398, 1444
BccI CCATC 4 cut(s) 142, 544, 572, 1252
BceAI ACGGC 2 cut(s) 112, 230
BciT130I CCWGG 5 cut(s) 588, 639, 942, 1131, 1371
BclI TGATCA 1 cut(s) 978
BcoDI GTCTC 2 cut(s) 204, 413
BfaI CTAG 3 cut(s) 555, 575, 1538
BfmI CTRYAG 2 cut(s) 900, 1022
BfoI RGCGCY 1 cut(s) 1428
BfrI CTTAAG 1 cut(s) 787
BglII AGATCT 1 cut(s) 783
BisI GCNGC 5 cut(s) 389, 752, 1299, 1412, 1458
BlsI GCNGC 5 cut(s) 390, 753, 1300, 1413, 1459
Bme1390I CCNGG 5 cut(s) 588, 639, 942, 1131, 1371
Bme18I GGWCC 2 cut(s) 179, 653
BmgBI CACGTC 1 cut(s) 144
BmgT120I GGNCC 3 cut(s) 179, 653, 933
BmiI GGNNCC 5 cut(s) 488, 617, 934, 1128, 1242
BmrFI CCNGG 5 cut(s) 588, 639, 942, 1131, 1371
BmrI ACTGGG 2 cut(s) 756, 853
BmsI GCATC 4 cut(s) 317, 567, 1240, 1289
BmtI GCTAGC 1 cut(s) 558
BmuI ACTGGG 2 cut(s) 756, 853
BpiI GAAGAC 3 cut(s) 380, 652, 1184
BpmI CTGGAG 2 cut(s) 800, 924
Bpu10I CCTNAGC 1 cut(s) 1254
BpuEI CTTGAG 4 cut(s) 186, 347, 646, 872
BsaAI YACGTR 1 cut(s) 1306
BsaBI GATNNNNATC 2 cut(s) 1239, 1488
BsaI GGTCTC 1 cut(s) 204
BsaJI CCNNGG 3 cut(s) 290, 586, 957
BsaWI WCCGGW 1 cut(s) 561
BsaXI ACNNNNNCTCC 1 cut(s) 34
Bsc4I CCNNNNNNNGG 4 cut(s) 501, 902, 947, 1237
Bse118I RCCGGY 1 cut(s) 737
Bse1I ACTGG 4 cut(s) 448, 656, 751, 848
Bse3DI GCAATG 1 cut(s) 1406
Bse8I GATNNNNATC 2 cut(s) 1239, 1488
BseAI TCCGGA 1 cut(s) 561
BseBI CCWGG 5 cut(s) 588, 639, 942, 1131, 1371
BseDI CCNNGG 3 cut(s) 290, 586, 957
BseGI GGATG 6 cut(s) 210, 303, 558, 716, 1091, 1264
BseJI GATNNNNATC 2 cut(s) 1239, 1488
BseLI CCNNNNNNNGG 4 cut(s) 501, 902, 947, 1237
BseMI GCAATG 1 cut(s) 1406
BseMII CTCAG 5 cut(s) 873, 914, 1245, 1298, 1352
BseNI ACTGG 4 cut(s) 448, 656, 751, 848
BseRI GAGGAG 7 cut(s) 22, 25, 28, 76, 96, 99, 144
BseSI GKGCMC 1 cut(s) 491
BseXI GCAGC 5 cut(s) 375, 738, 1285, 1398, 1444
Bsh1236I CGCG 1 cut(s) 27
BshFI GGCC 1 cut(s) 935
BshNI GGYRCC 1 cut(s) 486
BsiSI CCGG 3 cut(s) 562, 738, 1493
BslFI GGGAC 1 cut(s) 963
BslI CCNNNNNNNGG 4 cut(s) 501, 902, 947, 1237
BsmAI GTCTC 2 cut(s) 204, 413
BsmFI GGGAC 1 cut(s) 963
BsnI GGCC 1 cut(s) 935
Bso31I GGTCTC 1 cut(s) 204
Bsp1286I GDGCHC 1 cut(s) 491
Bsp13I TCCGGA 1 cut(s) 561
Bsp1407I TGTACA 1 cut(s) 1431
Bsp19I CCATGG 1 cut(s) 957
BspACI CCGC 1 cut(s) 1329
BspANI GGCC 1 cut(s) 935
BspCNI CTCAG 5 cut(s) 872, 915, 1246, 1297, 1353
BspEI TCCGGA 1 cut(s) 561
BspFNI CGCG 1 cut(s) 27
BspHI TCATGA 2 cut(s) 271, 756
BspLI GGNNCC 5 cut(s) 488, 617, 934, 1128, 1242
BspOI GCTAGC 1 cut(s) 558
BspPI GGATC 9 cut(s) 406, 445, 1121, 1134, 1235, 1248, 1370, 1491, 1575
BspT107I GGYRCC 1 cut(s) 486
BspTI CTTAAG 1 cut(s) 787
BspTNI GGTCTC 1 cut(s) 204
BsrDI GCAATG 1 cut(s) 1406
BsrFI RCCGGY 1 cut(s) 737
BsrGI TGTACA 1 cut(s) 1431
BsrI ACTGG 4 cut(s) 448, 656, 751, 848
BssAI RCCGGY 1 cut(s) 737
BssECI CCNNGG 3 cut(s) 290, 586, 957
BssT1I CCWWGG 2 cut(s) 290, 957
Bst2UI CCWGG 5 cut(s) 588, 639, 942, 1131, 1371
Bst4CI ACNGT 4 cut(s) 47, 319, 901, 1518
Bst6I CTCTTC 3 cut(s) 65, 119, 178
BstAFI CTTAAG 1 cut(s) 787
BstAUI TGTACA 1 cut(s) 1431
BstBAI YACGTR 1 cut(s) 1306
BstC8I GCNNGC 4 cut(s) 556, 1164, 1215, 1278
BstDEI CTNAG 5 cut(s) 859, 923, 1254, 1284, 1361
BstDSI CCRYGG 1 cut(s) 957
BstEII GGTNACC 2 cut(s) 581, 847
BstENI CCTNNNNNAGG 2 cut(s) 900, 1235
BstF5I GGATG 6 cut(s) 210, 303, 558, 716, 1091, 1264
BstFNI CGCG 1 cut(s) 27
BstH2I RGCGCY 1 cut(s) 1428
BstHHI GCGC 2 cut(s) 29, 1427
BstMAI GTCTC 2 cut(s) 204, 413
BstMWI GCNNNNNNNGC 1 cut(s) 1298
BstNI CCWGG 5 cut(s) 588, 639, 942, 1131, 1371
BstPI GGTNACC 2 cut(s) 581, 847
BstSCI CCNGG 5 cut(s) 586, 637, 940, 1129, 1369
BstSFI CTRYAG 2 cut(s) 900, 1022
BstSLI GKGCMC 1 cut(s) 491
BstSNI TACGTA 1 cut(s) 1306
BstUI CGCG 1 cut(s) 27
BstV1I GCAGC 5 cut(s) 375, 738, 1285, 1398, 1444
BstV2I GAAGAC 3 cut(s) 380, 652, 1184
BstX2I RGATCY 4 cut(s) 437, 783, 1126, 1240
BstYI RGATCY 4 cut(s) 437, 783, 1126, 1240
BsuRI GGCC 1 cut(s) 935
BtgI CCRYGG 1 cut(s) 957
BtrI CACGTC 1 cut(s) 144
BtsCI GGATG 6 cut(s) 210, 303, 558, 716, 1091, 1264
BtsI GCAGTG 1 cut(s) 12
BtsIMutI CAGTG 3 cut(s) 12, 1292, 1523
Cac8I GCNNGC 4 cut(s) 556, 1164, 1215, 1278
CciI TCATGA 2 cut(s) 271, 756
CfoI GCGC 2 cut(s) 29, 1427
Cfr10I RCCGGY 1 cut(s) 737
Cfr13I GGNCC 3 cut(s) 179, 653, 933
CseI GACGC 1 cut(s) 1189
CsiI ACCWGGT 1 cut(s) 1369
Csp6I GTAC 3 cut(s) 1307, 1432, 1498
CviAII CATG 6 cut(s) 272, 534, 757, 946, 958, 1156
CviQI GTAC 3 cut(s) 1307, 1432, 1498
DdeI CTNAG 5 cut(s) 859, 923, 1254, 1284, 1361
DrdI GACNNNNNNGTC 1 cut(s) 1186
DseDI GACNNNNNNGTC 1 cut(s) 1186
Eam1104I CTCTTC 3 cut(s) 65, 119, 178
EarI CTCTTC 3 cut(s) 65, 119, 178
Eco105I TACGTA 1 cut(s) 1306
Eco130I CCWWGG 2 cut(s) 290, 957
Eco31I GGTCTC 1 cut(s) 204
Eco47I GGWCC 2 cut(s) 179, 653
Eco47III AGCGCT 1 cut(s) 1426
Eco91I GGTNACC 2 cut(s) 581, 847
EcoNI CCTNNNNNAGG 2 cut(s) 900, 1235
EcoO65I GGTNACC 2 cut(s) 581, 847
EcoRI GAATTC 2 cut(s) 185, 275
EcoRII CCWGG 5 cut(s) 586, 637, 940, 1129, 1369
EcoT14I CCWWGG 2 cut(s) 290, 957
ErhI CCWWGG 2 cut(s) 290, 957
FaeI CATG 6 cut(s) 275, 537, 760, 949, 961, 1159
FalI AAGNNNNNCTT 2 cut(s) 1032, 1064
FaqI GGGAC 1 cut(s) 963
FatI CATG 6 cut(s) 271, 533, 756, 945, 957, 1155
FbaI TGATCA 1 cut(s) 978
FblI GTMKAC 1 cut(s) 840
Fnu4HI GCNGC 5 cut(s) 389, 752, 1299, 1412, 1458
FokI GGATG 6 cut(s) 197, 310, 545, 703, 1078, 1271
Fsp4HI GCNGC 5 cut(s) 389, 752, 1299, 1412, 1458
FspBI CTAG 3 cut(s) 555, 575, 1538
GlaI GCGC 2 cut(s) 28, 1426
GluI GCNGC 5 cut(s) 389, 752, 1299, 1412, 1458
GsuI CTGGAG 2 cut(s) 800, 924
HaeII RGCGCY 1 cut(s) 1428
HaeIII GGCC 1 cut(s) 935
HapII CCGG 3 cut(s) 562, 738, 1493
HgaI GACGC 1 cut(s) 1189
HhaI GCGC 2 cut(s) 29, 1427
Hin1II CATG 6 cut(s) 275, 537, 760, 949, 961, 1159
Hin6I GCGC 2 cut(s) 27, 1425
HinP1I GCGC 2 cut(s) 27, 1425
HpaII CCGG 3 cut(s) 562, 738, 1493
HphI GGTGA 5 cut(s) 35, 123, 575, 1091, 1385
Hpy166II GTNNAC 4 cut(s) 23, 841, 920, 991
Hpy188I TCNGA 6 cut(s) 141, 385, 997, 1192, 1249, 1577
Hpy188III TCNNGA 9 cut(s) 177, 203, 272, 364, 562, 619, 757, 779, 1118
Hpy8I GTNNAC 4 cut(s) 23, 841, 920, 991
Hpy99I CGWCG 2 cut(s) 145, 251
HpyAV CCTTC 4 cut(s) 100, 257, 691, 1433
HpyCH4III ACNGT 4 cut(s) 47, 319, 901, 1518
HpyCH4IV ACGT 3 cut(s) 143, 428, 1305
HpyCH4V TGCA 4 cut(s) 308, 1280, 1301, 1460
HpyF10VI GCNNNNNNNGC 1 cut(s) 1298
HpyF3I CTNAG 5 cut(s) 859, 923, 1254, 1284, 1361
HpySE526I ACGT 3 cut(s) 143, 428, 1305
Hsp92II CATG 6 cut(s) 275, 537, 760, 949, 961, 1159
HspAI GCGC 2 cut(s) 27, 1425
Kpn2I TCCGGA 1 cut(s) 561
Ksp22I TGATCA 1 cut(s) 978
LmnI GCTCC 3 cut(s) 534, 905, 1233
Lsp1109I GCAGC 5 cut(s) 375, 738, 1285, 1398, 1444
LweI GCATC 4 cut(s) 317, 567, 1240, 1289
MabI ACCWGGT 1 cut(s) 1369
MaeI CTAG 3 cut(s) 555, 575, 1538
MaeII ACGT 3 cut(s) 143, 428, 1305
MaeIII GTNAC 6 cut(s) 41, 129, 570, 581, 847, 1170
MfeI CAATTG 2 cut(s) 192, 480
MflI RGATCY 4 cut(s) 437, 783, 1126, 1240
MhlI GDGCHC 1 cut(s) 491
MluCI AATT 9 cut(s) 185, 192, 275, 368, 480, 666, 672, 723, 878
MlyI GAGTC 6 cut(s) 415, 481, 934, 986, 992, 1021
MmeI TCCRAC 2 cut(s) 164, 449
MroI TCCGGA 1 cut(s) 561
MroXI GAANNNNTTC 2 cut(s) 267, 1044
MseI TTAA 5 cut(s) 465, 671, 693, 788, 1271
MslI CAYNNNNRTG 1 cut(s) 1285
MspCI CTTAAG 1 cut(s) 787
MspI CCGG 3 cut(s) 562, 738, 1493
MspR9I CCNGG 5 cut(s) 588, 639, 942, 1131, 1371
MunI CAATTG 2 cut(s) 192, 480
MvaI CCWGG 5 cut(s) 588, 639, 942, 1131, 1371
MvnI CGCG 1 cut(s) 27
MwoI GCNNNNNNNGC 1 cut(s) 1298
NcoI CCATGG 1 cut(s) 957
NheI GCTAGC 1 cut(s) 554
NlaIII CATG 6 cut(s) 275, 537, 760, 949, 961, 1159
NlaIV GGNNCC 5 cut(s) 488, 617, 934, 1128, 1242
NmuCI GTSAC 4 cut(s) 41, 129, 570, 581
PagI TCATGA 2 cut(s) 271, 756
PdmI GAANNNNTTC 2 cut(s) 267, 1044
PfeI GAWTC 4 cut(s) 224, 596, 971, 1332
PfoI TCCNGGA 1 cut(s) 1129
PkrI GCNGC 5 cut(s) 390, 753, 1300, 1413, 1459
PleI GAGTC 6 cut(s) 415, 480, 933, 986, 992, 1021
PpsI GAGTC 6 cut(s) 415, 480, 933, 986, 992, 1021
Ppu21I YACGTR 1 cut(s) 1306
PshBI ATTAAT 1 cut(s) 693
Psp1406I AACGTT 1 cut(s) 428
Psp6I CCWGG 5 cut(s) 586, 637, 940, 1129, 1369
PspEI GGTNACC 2 cut(s) 581, 847
PspGI CCWGG 5 cut(s) 586, 637, 940, 1129, 1369
PspN4I GGNNCC 5 cut(s) 488, 617, 934, 1128, 1242
PspPI GGNCC 3 cut(s) 179, 653, 933
PsuI RGATCY 4 cut(s) 437, 783, 1126, 1240
RsaI GTAC 3 cut(s) 1308, 1433, 1499
RsaNI GTAC 3 cut(s) 1307, 1432, 1498
RseI CAYNNNNRTG 1 cut(s) 1285
SaqAI TTAA 5 cut(s) 465, 671, 693, 788, 1271
SatI GCNGC 5 cut(s) 389, 752, 1299, 1412, 1458
Sau96I GGNCC 3 cut(s) 179, 653, 933
SchI GAGTC 6 cut(s) 415, 481, 934, 986, 992, 1021
ScrFI CCNGG 5 cut(s) 588, 639, 942, 1131, 1371
SduI GDGCHC 1 cut(s) 491
SexAI ACCWGGT 1 cut(s) 1369
SfaNI GCATC 4 cut(s) 317, 567, 1240, 1289
SfcI CTRYAG 2 cut(s) 900, 1022
SinI GGWCC 2 cut(s) 179, 653
SmiMI CAYNNNNRTG 1 cut(s) 1285
SmlI CTYRAG 5 cut(s) 201, 362, 625, 787, 887
SmoI CTYRAG 5 cut(s) 201, 362, 625, 787, 887
SnaBI TACGTA 1 cut(s) 1306
Sse9I AATT 9 cut(s) 185, 192, 275, 368, 480, 666, 672, 723, 878
SsiI CCGC 1 cut(s) 1329
SspI AATATT 1 cut(s) 1205
SspMI CTAG 3 cut(s) 555, 575, 1538
StyD4I CCNGG 5 cut(s) 586, 637, 940, 1129, 1369
StyI CCWWGG 2 cut(s) 290, 957
TaaI ACNGT 4 cut(s) 47, 319, 901, 1518
TaiI ACGT 3 cut(s) 146, 431, 1308
TaqI TCGA 3 cut(s) 261, 475, 1108
TasI AATT 9 cut(s) 185, 192, 275, 368, 480, 666, 672, 723, 878
TatI WGTACW 1 cut(s) 1431
TfiI GAWTC 4 cut(s) 224, 596, 971, 1332
Tru1I TTAA 5 cut(s) 465, 671, 693, 788, 1271
Tru9I TTAA 5 cut(s) 465, 671, 693, 788, 1271
TscAI CASTG 3 cut(s) 12, 1292, 1523
TseFI GTSAC 4 cut(s) 41, 129, 570, 581
TseI GCWGC 5 cut(s) 388, 751, 1298, 1411, 1457
Tsp45I GTSAC 4 cut(s) 41, 129, 570, 581
TspDTI ATGAA 4 cut(s) 82, 260, 288, 1467
TspRI CASTG 3 cut(s) 12, 1292, 1523
Vha464I CTTAAG 1 cut(s) 787
VpaK11BI GGWCC 2 cut(s) 179, 653
VspI ATTAAT 1 cut(s) 693
XagI CCTNNNNNAGG 2 cut(s) 900, 1235
XapI RAATTY 2 cut(s) 185, 275
XcmI CCANNNNNNNNNTGG 1 cut(s) 943
XmiI GTMKAC 1 cut(s) 840
XmnI GAANNNNTTC 2 cut(s) 267, 1044
XspI CTAG 3 cut(s) 555, 575, 1538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.