Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0014421

Physical Location & Seq

Genomic Coordinates Forward (+)

24677121 .. 24678580

Gene Structure 1460 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G66230.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 849 bp

ATGGGGAGACAACCATGCTGTGACAAAGTAGGGTTGAAGAAAGGACCATGGACTGCAGAAGAGGATAGGAAGCTCATAAACTTCATCCTTACCAATGGACAATGTTGTTGGAGAGCTGTTCCTAAGCTTTCTGGTCTTCTTAGGTGTGGCAAGAGTTGCAGACTTCGTTGGACTAACTATCTTAGACCAGACCTTAAGAGAGGTCTTCTCTCTGATTACGAAGAGAAGATGGTCATTGATCTCCATTCCCAGCTTGGAAACAGGTGGTCAAAGATAGCTTCTCATTTACCAGGAAGAACAGACAACGAAATCAAGAATCATTGGAACACTCACATCAAGAAGAAGTTGAGGAAAATGGGGATTGATCCTCTTACACATAAACCACTCTCTATCGTCGAAAAAGAAGACGAAGAACCCTTAAAGAAGCTACAGAATAATACAGTTCCTTTTCAAGAAACAATGGAGCGTCCTTTAGAGAACAACATCAAGAACATATCAAGACTTGAAGAGTCTTTAGGTGATGATCAATTCATGGAGATAAATCTTGAGTATGGTGTCGAAGATGTCCCTCTTATTGAAACAGAGTCTTTAGACCTTATCTGCAGCAATTCAACAATGTCTTCATCCACGTCCACATCTTCGCATTCTTCTAATGATTCGAGTTTCTTGAAGGATTTGCAGTTCCCGGAGTTCGAGTGGTCCGACTATGGTAATAGTAATAATGATAATAATAATGGTGTGGACAACATTATAGAGAACAATATGATGAGCCTGTGGGAAATTAGTGACTTTAGCAGTTTGGATTTGCTGCTTAATGATGAGAGTTCTTCCACTTTTGGGTTGTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000976 GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001134 GO:0001135 GO:0001158 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032502 GO:0035326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044087 GO:0044212 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0047484 GO:0048518 GO:0048519 GO:0048523 GO:0048583 GO:0048584 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0060255 GO:0065007 GO:0080090 GO:0080134 GO:0097159 GO:0140110 GO:1901000 GO:1901002 GO:1901363 GO:1902679 GO:1903338 GO:1903506 GO:1903507 GO:1990837 GO:2000112 GO:2000113 GO:2000652 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Myb_DNA-binding

Protein Analysis

282

Amino Acids

32.34

Weight (kDa)

5.13

Isoelectric Point (pI)

53.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Myb_DNA-binding	PF00249	14 - 61	3.2e-14	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	17 - 75	2.9e-07	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	72 - 118	9.4e-09	Myb-like DNA-binding domain
Myb_DNA-binding	PF00249	73 - 111	1.8e-15	Myb-like DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0014421)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G66230
fragaria_vesca	FvH4_1g30290
malus_domestica	MD00G1116400.v1.1 MD09G1271400.v1.1
prunus_persica	Prupe.3G017100_v2.0.a1
pyrus_communis	pycom09g18240 pycom17g26820
rosa_chinensis	RchiOBHm_Chr3g0491061
rosa_laevigata	RLG00000022867
rosa_multiflora	Rmu_sc0005038.1_g000005 Rmu_ssc0000386.1_g000051
rosa_roxburghii	Rroxscaffold_6G00393250
rosa_rugosa	Rorug03G0245700
rosa_samantha	Rh3AG296000 Rh3CG329100 Rh3DG329700
rosa_wichuraiana	Rw3G026120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AclWI	GGATC	1 cut(s)	359
AfiI	CCNNNNNNNGG	1 cut(s)	837
AflII	CTTAAG	1 cut(s)	194
AgsI	TTSAA	6 cut(s)	37, 452, 506, 578, 612, 670
AjiI	CACGTC	1 cut(s)	630
AjnI	CCWGG	1 cut(s)	289
AloI	GAACNNNNNNTCC	2 cut(s)	665, 697
AluBI	AGCT	6 cut(s)	73, 116, 127, 253, 278, 427
AluI	AGCT	6 cut(s)	73, 116, 127, 253, 278, 427
AlwI	GGATC	1 cut(s)	359
ApeKI	GCWGC	2 cut(s)	603, 808
AspS9I	GGNCC	2 cut(s)	44, 699
AsuC2I	CCSGG	1 cut(s)	686
AsuHPI	GGTGA	1 cut(s)	530
AvaII	GGWCC	2 cut(s)	44, 699
BbsI	GAAGAC	4 cut(s)	128, 197, 411, 612
BbvI	GCAGC	2 cut(s)	615, 795
BccI	CCATC	1 cut(s)	223
BciT130I	CCWGG	1 cut(s)	291
BclI	TGATCA	1 cut(s)	523
BcnI	CCSGG	1 cut(s)	686
BfmI	CTRYAG	3 cut(s)	54, 428, 601
BfrI	CTTAAG	1 cut(s)	194
BisI	GCNGC	2 cut(s)	604, 809
BlsI	GCNGC	2 cut(s)	605, 810
Bme1390I	CCNGG	2 cut(s)	291, 686
Bme18I	GGWCC	2 cut(s)	44, 699
BmgBI	CACGTC	1 cut(s)	630
BmgT120I	GGNCC	2 cut(s)	44, 699
BmrFI	CCNGG	2 cut(s)	291, 686
BpiI	GAAGAC	4 cut(s)	128, 197, 411, 612
BplI	GAGNNNNNCTC	2 cut(s)	192, 224
Bpu10I	CCTNAGC	1 cut(s)	123
BpuEI	CTTGAG	1 cut(s)	566
BpuMI	CCSGG	1 cut(s)	686
BsaJI	CCNNGG	1 cut(s)	47
BsaXI	ACNNNNNCTCC	2 cut(s)	680, 710
Bsc4I	CCNNNNNNNGG	1 cut(s)	837
BseBI	CCWGG	1 cut(s)	291
BseDI	CCNNGG	1 cut(s)	47
BseGI	GGATG	2 cut(s)	84, 623
BseLI	CCNNNNNNNGG	1 cut(s)	837
BseXI	GCAGC	2 cut(s)	615, 795
BseYI	CCCAGC	1 cut(s)	249
BsiSI	CCGG	1 cut(s)	686
BslFI	GGGAC	1 cut(s)	551
BslI	CCNNNNNNNGG	1 cut(s)	837
BsmFI	GGGAC	1 cut(s)	551
BsmI	GAATGC	1 cut(s)	643
Bsp143I	GATC	3 cut(s)	238, 364, 523
Bsp19I	CCATGG	1 cut(s)	47
BspMAI	CTGCAG	2 cut(s)	58, 605
BspPI	GGATC	1 cut(s)	359
BspTI	CTTAAG	1 cut(s)	194
BssECI	CCNNGG	1 cut(s)	47
BssMI	GATC	3 cut(s)	238, 364, 523
BssT1I	CCWWGG	1 cut(s)	47
Bst2UI	CCWGG	1 cut(s)	291
Bst4CI	ACNGT	1 cut(s)	442
Bst6I	CTCTTC	3 cut(s)	54, 216, 501
BstAFI	CTTAAG	1 cut(s)	194
BstAPI	GCANNNNNTGC	1 cut(s)	156
BstDEI	CTNAG	3 cut(s)	123, 140, 182
BstDSI	CCRYGG	1 cut(s)	47
BstF5I	GGATG	2 cut(s)	84, 623
BstKTI	GATC	3 cut(s)	241, 367, 526
BstMBI	GATC	3 cut(s)	238, 364, 523
BstMWI	GCNNNNNNNGC	1 cut(s)	156
BstNI	CCWGG	1 cut(s)	291
BstSCI	CCNGG	2 cut(s)	289, 684
BstSFI	CTRYAG	3 cut(s)	54, 428, 601
BstV1I	GCAGC	2 cut(s)	615, 795
BstV2I	GAAGAC	4 cut(s)	128, 197, 411, 612
BtgI	CCRYGG	1 cut(s)	47
BtrI	CACGTC	1 cut(s)	630
BtsCI	GGATG	2 cut(s)	84, 623
Cfr13I	GGNCC	2 cut(s)	44, 699
CseI	GACGC	1 cut(s)	455
CviAII	CATG	3 cut(s)	15, 48, 532
CviJI	RGCY	7 cut(s)	73, 116, 127, 253, 278, 427, 771
CviKI_1	RGCY	7 cut(s)	73, 116, 127, 253, 278, 427, 771
DdeI	CTNAG	3 cut(s)	123, 140, 182
DpnI	GATC	3 cut(s)	240, 366, 525
DpnII	GATC	3 cut(s)	238, 364, 523
Eam1104I	CTCTTC	3 cut(s)	54, 216, 501
EarI	CTCTTC	3 cut(s)	54, 216, 501
Eco130I	CCWWGG	1 cut(s)	47
Eco47I	GGWCC	2 cut(s)	44, 699
EcoRII	CCWGG	1 cut(s)	289
EcoT14I	CCWWGG	1 cut(s)	47
ErhI	CCWWGG	1 cut(s)	47
FaeI	CATG	3 cut(s)	18, 51, 535
FaqI	GGGAC	1 cut(s)	551
FatI	CATG	3 cut(s)	14, 47, 531
FbaI	TGATCA	1 cut(s)	523
Fnu4HI	GCNGC	2 cut(s)	604, 809
FokI	GGATG	2 cut(s)	71, 610
Fsp4HI	GCNGC	2 cut(s)	604, 809
GluI	GCNGC	2 cut(s)	604, 809
GsaI	CCCAGC	1 cut(s)	253
HapII	CCGG	1 cut(s)	686
HgaI	GACGC	1 cut(s)	455
Hin1II	CATG	3 cut(s)	18, 51, 535
HindIII	AAGCTT	1 cut(s)	125
HinfI	GANTC	4 cut(s)	316, 509, 584, 656
HpaII	CCGG	1 cut(s)	686
HphI	GGTGA	1 cut(s)	530
Hpy166II	GTNNAC	2 cut(s)	633, 742
Hpy188I	TCNGA	2 cut(s)	214, 703
Hpy188III	TCNNGA	7 cut(s)	313, 337, 452, 487, 498, 545, 667
Hpy8I	GTNNAC	2 cut(s)	633, 742
Hpy99I	CGWCG	1 cut(s)	398
HpyAV	CCTTC	1 cut(s)	664
HpyCH4III	ACNGT	1 cut(s)	442
HpyCH4IV	ACGT	1 cut(s)	629
HpyCH4V	TGCA	4 cut(s)	56, 159, 603, 679
HpyF10VI	GCNNNNNNNGC	1 cut(s)	156
HpyF3I	CTNAG	3 cut(s)	123, 140, 182
HpySE526I	ACGT	1 cut(s)	629
Hsp92II	CATG	3 cut(s)	18, 51, 535
Ksp22I	TGATCA	1 cut(s)	523
Kzo9I	GATC	3 cut(s)	238, 364, 523
LmnI	GCTCC	1 cut(s)	463
LpnPI	CCDG	8 cut(s)	117, 201, 247, 263, 276, 303, 699, 785
Lsp1109I	GCAGC	2 cut(s)	615, 795
MaeII	ACGT	1 cut(s)	629
MaeIII	GTNAC	2 cut(s)	20, 785
MalI	GATC	3 cut(s)	240, 366, 525
MboI	GATC	3 cut(s)	238, 364, 523
MluCI	AATT	3 cut(s)	527, 607, 780
MlyI	GAGTC	2 cut(s)	518, 593
MmeI	TCCRAC	3 cut(s)	89, 149, 726
MnlI	CCTC	5 cut(s)	55, 194, 342, 378, 579
MseI	TTAA	3 cut(s)	195, 419, 813
MspCI	CTTAAG	1 cut(s)	194
MspI	CCGG	1 cut(s)	686
MspR9I	CCNGG	2 cut(s)	291, 686
Mva1269I	GAATGC	1 cut(s)	643
MvaI	CCWGG	1 cut(s)	291
MwoI	GCNNNNNNNGC	1 cut(s)	156
NciI	CCSGG	1 cut(s)	686
NcoI	CCATGG	1 cut(s)	47
NdeII	GATC	3 cut(s)	238, 364, 523
NlaIII	CATG	3 cut(s)	18, 51, 535
NmuCI	GTSAC	2 cut(s)	20, 785
PcsI	WCGNNNNNNNCGW	1 cut(s)	699
PctI	GAATGC	1 cut(s)	643
PfeI	GAWTC	2 cut(s)	316, 656
PfoI	TCCNGGA	1 cut(s)	684
PkrI	GCNGC	2 cut(s)	605, 810
PleI	GAGTC	2 cut(s)	517, 592
PpsI	GAGTC	2 cut(s)	517, 592
Psp6I	CCWGG	1 cut(s)	289
PspFI	CCCAGC	1 cut(s)	249
PspGI	CCWGG	1 cut(s)	289
PspPI	GGNCC	2 cut(s)	44, 699
PstI	CTGCAG	2 cut(s)	58, 605
SaqAI	TTAA	3 cut(s)	195, 419, 813
SatI	GCNGC	2 cut(s)	604, 809
Sau3AI	GATC	3 cut(s)	238, 364, 523
Sau96I	GGNCC	2 cut(s)	44, 699
SchI	GAGTC	2 cut(s)	518, 593
ScrFI	CCNGG	2 cut(s)	291, 686
SfcI	CTRYAG	3 cut(s)	54, 428, 601
SinI	GGWCC	2 cut(s)	44, 699
SmlI	CTYRAG	2 cut(s)	194, 545
SmoI	CTYRAG	2 cut(s)	194, 545
Sse9I	AATT	3 cut(s)	527, 607, 780
StyD4I	CCNGG	2 cut(s)	289, 684
StyI	CCWWGG	1 cut(s)	47
TaaI	ACNGT	1 cut(s)	442
TaiI	ACGT	1 cut(s)	632
TaqI	TCGA	4 cut(s)	396, 558, 659, 693
TasI	AATT	3 cut(s)	527, 607, 780
TfiI	GAWTC	2 cut(s)	316, 656
Tru1I	TTAA	3 cut(s)	195, 419, 813
Tru9I	TTAA	3 cut(s)	195, 419, 813
TseFI	GTSAC	2 cut(s)	20, 785
TseI	GCWGC	2 cut(s)	603, 808
Tsp45I	GTSAC	2 cut(s)	20, 785
TspDTI	ATGAA	3 cut(s)	73, 520, 612
Vha464I	CTTAAG	1 cut(s)	194
VpaK11BI	GGWCC	2 cut(s)	44, 699
XcmI	CCANNNNNNNNNTGG	1 cut(s)	251

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.