Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000003

Orthogroup ID

OG0001967

Physical Location & Seq

Genomic Coordinates Forward (+)

22988777 .. 22992601

Gene Structure 3825 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug03G0245700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2325 bp

ATGCAGAGGCCTCCACCGGAAGACTTTGCTTTGAAGGAGACCAAACCCCATCTCGGCGGGGGGAGGATCTCCGGCGACAAGCACACGAGCACCTATGACCTCGTTGAGCAGATGCAGTACCTCTATGTTCGGGTTGTAAAGGCCAAGGACTTGCCGTCCAAGGATGTGACCGGAAGCTGTGACCCTTATGTTGAAGTTAAGCTGGGAAACTACAAGGGTACAACTCGGCATTTTGAGAAGAAGACCCATCCGGAGTGGAACCAGGTGTTTGCATTCTCGAAGGACCGGATCCAGGCTTCGGCCCTTGAGGTGATTGTGAAGGACAAGGATCTTTTGAAGGATGATTTCATTGGCCACGTTATTTTTGACCTGAATGAGGTTCCGAAAAGGGTTCCACCTGATAGTCCCCTGGCACCACAATGGTATAGACTGGAGGATAGGAGCGGGGGCAAGGCTAGGGGTGAGCTGATGTTGGCTGTTTGGATGGGCACTCAAGCTGATGAAGCATTTCCTGAAGCATGGCATTCCGATGCTGCTACAGTTAGTGGGGCTGACAGTCTGTCAAACATTCGATCAAAGGTGTATCTCTCTCCCAAGCTATGGTATTTGAGGGTTAATGTGATTGAGGCTCAGGATTTGATGCCGAGCGATAAAGGCAGGTATCCGGAAGTTTATGTGAAGGCTATCCTGGGAAATCAGGCTTTGAGAACTCGAATTTCACAAAGCAGGAGTATAAATCCCATGTGGAATGAAGATTTAATGTTTGTAGCATCAGAGCCTTTTGAGGAGCCATTGATTTTAAGTGTGGAAGATAGAGTTGCGCCAAACAAGGATGAAGTTCTGGGGAGGTGTGCTATACCTTTGCAGTATGTGGCCCGAAGGTATGATCATAAACCTGTGAACACTAGCTGGCACAATCTTGAGAAGCACATTATTGTAGAAGGGGAAAAGAAGAAGGATATTAAGTTTGCAAGCAGGATTCATATGAGGATCTGTCTAGAAGGTGGCTACCATGTGCTTGATGAGTCAACACACTATAGTAGTGATCTTAGACCGACAGCCAAACCGTTGTGGAAGTCCAGCATTGGGGTTCTGGAAGTGGGAATCCTGAATGCTCAGGGGTTGATGCCAATGAAGACAATAGACGGTAGGGGAACAACAGATGCTTACTGCGTTGCAAAATATGGGCAGAAGTGGGTTCGGACAAGAACCATTATCGATAGCTTTGCTCCCAGGTGGAATGAGCAATACACTTGGGAAGTTTTTGATCCTTGCACTGTCATTACAATTGGGGTCTTTGATAACTGTCATTTGCATGGAGGAGAGAAGGCTGCTGCGGCAAAGGATTCACGAATCGGGAAGGTCAGGATTCGTCTCTCTACTCTTGAAACTGATCGGGTTTACACACACTCTTATCCTCTTCTGGTTCTGCACCCCAATGGTGTTAAGAAGATGGGTGAAATTCATATGGCTGTGAGGTTCACCTGCTCTTCTTTGCTGAACATGATGCATATGTACTCGCAACCACTGTTGCCCAAAATGCACTATCTTCATCCATTAACTGTAAGCCAGCTTGATAGCTTGAGGCACCAGGCCACTCTGATTGTATCAGTGAGGCTGAGCCGTGCTGAACCGCCCTTGAGAAAGGAGGTGGTCGAGTATATGCTGGATGTGGGTTCCCACATGTGGAGTATGAGAAGAAGCAAAGCTAATTTCTTCAGGATTATGAACGTTCTTGGTGGATTAATTGCTGTTGGAAAATGGTTTGATCAGATCTGTAATTGGAAAAACCCCATCACTACTGTGCTTATTCATATCCTGTTCATAATTCTGGTCATGTACCCAGAGCTGATATTACCTACAATTTTTCTCTACCTCTTTTTGATTGGAGTCTGGTACTACAGATGGAGGCCGAGGCATCCTCCTCACATGGACACTCGTCTCTCTCACGCAGATTCTGCACATCCTGATGAACTTGATGAAGAATTTGATACCTTCCCAACTTCACGACCTTCTGACATAGTGAGGATGAGATATGACAGATTGAGGAGTATTGCTGGGAGGATCCAGACAGTGGTTGGTGACCTGGCTACTCAAGGGGAGAGGCTGCAATCATTGTTGAGCTGGCGAGACCCCAGAGCTACAGCTCTGTTTGTGCTTTTCTGTCTGATTGCTGCCATTGTTCTGTATGTTACTCCATTCCAGATTGTGGCACTTCTCGCTGGATTCTATGTGTTAAGGCACCCAAGATTCCGCCATAAGCTTCCATCAGTGCCGTTGAATTTCTTCAGGAGGTTGCCGGCTAGGACCGACTGCATGCTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001882 GO:0001883 GO:0002252 GO:0002376 GO:0003674 GO:0005488 GO:0005525 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009615 GO:0017076 GO:0019001 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0043207 GO:0050896 GO:0051607 GO:0051704 GO:0051707 GO:0097159 GO:0097367 GO:0098542 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Arf

Protein Analysis

774

Amino Acids

88.88

Weight (kDa)

9.11

Isoelectric Point (pI)

47.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	40 - 143	2.8e-23	C2 domain
C2	PF00168	201 - 306	5.3e-20	C2 domain
C2	PF00168	363 - 474	3.6e-20	C2 domain
Pex24p	PF06398	592 - 754	6.9e-07	Integral peroxisomal membrane peroxin
PRT_C	PF08372	619 - 774	5.1e-83	Plant phosphoribosyltransferase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001967)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G37680 AT5G37680
fragaria_vesca	FvH4_1g30280 FvH4_1g30280 FvH4_1g30280 FvH4_2g03780
malus_domestica	MD05G1101000.v1.1 MD09G1271300.v1.1 MD17G1269600.v1.1
prunus_persica	Prupe.3G017200_v2.0.a1 Prupe.3G017200_v2.0.a1 Prupe.3G017200_v2.0.a1 Prupe.3G017200_v2.0.a1 Prupe.3G017200_v2.0.a1 Prupe.3G017200_v2.0.a1 Prupe.8G145900_v2.0.a1 Prupe.8G145900_v2.0.a1
pyrus_communis	pycom05g09970 pycom09g18230 pycom15g26060 pycom17g26810
rosa_chinensis	RchiOBHm_Chr3g0491081 RchiOBHm_Chr6g0252801
rosa_laevigata	RLG00000017639
rosa_multiflora	Rmu_sc0000829.1_g000043 Rmu_sc0005038.1_g000013 Rmu_ssc0000386.1_g000060
rosa_roxburghii	Rroxscaffold_6G00393230 Rroxscaffold_7G00212420
rosa_rugosa	Rorug03G0245700 Rorug03G0245700 Rorug03G0245800 Rorug03G0245900 Rorug05G0538800 Rorug05G0538800
rosa_samantha	Rh3AG296100 Rh3BG332100 Rh3CG329200 Rh3DG329800 Rh6AG054800 Rh6BG048800 Rh6CG047700 Rh6DG043200
rosa_wichuraiana	Rw3G026130 Rw6G004950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1493
Acc36I	ACCTGC	2 cut(s)	648, 1493
AccB1I	GGYRCC	3 cut(s)	412, 1587, 2241
AccB7I	CCANNNNNTGG	3 cut(s)	600, 2074, 2209
AccBSI	CCGCTC	1 cut(s)	444
AccIII	TCCGGA	2 cut(s)	250, 664
AciI	CCGC	5 cut(s)	57, 444, 1337, 1634, 2254
AclI	AACGTT	1 cut(s)	1731
AclWI	GGATC	8 cut(s)	74, 283, 296, 336, 998, 1262, 2059, 2072
AcoI	YGGCCR	1 cut(s)	352
AcsI	RAATTY	4 cut(s)	714, 1461, 1985, 2281
AcuI	CTGAAG	3 cut(s)	534, 1702, 2272
AfaI	GTAC	5 cut(s)	119, 220, 1517, 1841, 1898
AfiI	CCNNNNNNNGG	9 cut(s)	53, 292, 298, 376, 600, 1086, 1686, 2074, 2209
AflIII	ACRYGT	1 cut(s)	1683
AgsI	TTSAA	5 cut(s)	34, 194, 337, 1388, 2281
AhdI	GACNNNNNGTC	2 cut(s)	154, 559
AjnI	CCWGG	7 cut(s)	261, 291, 408, 687, 1232, 1590, 2085
AleI	CACNNNNGTG	1 cut(s)	1802
Alw21I	GWGCWC	1 cut(s)	92
Alw26I	GTCTC	4 cut(s)	32, 1379, 1946, 2124
AlwI	GGATC	8 cut(s)	74, 283, 296, 336, 998, 1262, 2059, 2072
AlwNI	CAGNNNCTG	1 cut(s)	1958
Aor13HI	TCCGGA	2 cut(s)	250, 664
AoxI	GGCC	7 cut(s)	8, 141, 300, 352, 873, 1593, 1910
ApeKI	GCWGC	5 cut(s)	533, 1331, 1334, 2107, 2174
ApoI	RAATTY	4 cut(s)	714, 1461, 1985, 2281
AseI	ATTAAT	1 cut(s)	1745
Asp700I	GAANNNNTTC	2 cut(s)	507, 2285
AspLEI	GCGC	1 cut(s)	823
AspS9I	GGNCC	4 cut(s)	283, 301, 874, 2307
AsuHPI	GGTGA	5 cut(s)	322, 473, 1469, 1474, 2093
AvaII	GGWCC	2 cut(s)	283, 2307
BaeGI	GKGCMC	1 cut(s)	491
BalI	TGGCCA	1 cut(s)	354
BamHI	GGATCC	2 cut(s)	288, 2064
BanI	GGYRCC	3 cut(s)	412, 1587, 2241
BauI	CACGAG	1 cut(s)	85
BbsI	GAAGAC	3 cut(s)	27, 248, 1142
Bbv12I	GWGCWC	1 cut(s)	92
BbvI	GCAGC	5 cut(s)	520, 1318, 1321, 2094, 2161
BccI	CCATC	7 cut(s)	57, 255, 478, 1447, 1802, 1899, 2275
BceAI	ACGGC	3 cut(s)	139, 1608, 2260
BcgI	CGANNNNNNTGC	2 cut(s)	1208, 1242
BciT130I	CCWGG	7 cut(s)	263, 293, 410, 689, 1234, 1592, 2087
BciVI	GTATCC	1 cut(s)	672
BclI	TGATCA	2 cut(s)	886, 1768
BcoDI	GTCTC	4 cut(s)	32, 1379, 1946, 2124
BfaI	CTAG	4 cut(s)	456, 906, 998, 2304
BfmI	CTRYAG	4 cut(s)	537, 1036, 1900, 2142
BfuAI	ACCTGC	2 cut(s)	648, 1493
BfuI	GTATCC	1 cut(s)	672
BglII	AGATCT	1 cut(s)	1773
BisI	GCNGC	6 cut(s)	534, 1332, 1335, 1338, 2108, 2175
BlpI	GCTNAGC	1 cut(s)	1619
BlsI	GCNGC	6 cut(s)	535, 1333, 1336, 1339, 2109, 2176
Bme1390I	CCNGG	7 cut(s)	263, 293, 410, 689, 1234, 1592, 2087
Bme18I	GGWCC	2 cut(s)	283, 2307
BmeRI	GACNNNNNGTC	2 cut(s)	154, 559
BmgT120I	GGNCC	4 cut(s)	283, 301, 874, 2307
BmrFI	CCNGG	7 cut(s)	263, 293, 410, 689, 1234, 1592, 2087
BmsI	GCATC	8 cut(s)	102, 520, 630, 779, 1116, 1153, 1497, 1927
BoxI	GACNNNNGTC	1 cut(s)	1938
BpiI	GAAGAC	3 cut(s)	27, 248, 1142
BplI	GAGNNNNNCTC	2 cut(s)	1906, 1938
BpmI	CTGGAG	1 cut(s)	452
Bpu10I	CCTNAGC	2 cut(s)	630, 1116
Bpu1102I	GCTNAGC	1 cut(s)	1619
BpuEI	CTTGAG	6 cut(s)	326, 477, 941, 1603, 1660, 2079
Bsa29I	ATCGAT	1 cut(s)	1218
BsaI	GGTCTC	2 cut(s)	32, 2124
BsaJI	CCNNGG	6 cut(s)	144, 159, 408, 688, 1232, 1913
BsaWI	WCCGGW	5 cut(s)	16, 170, 250, 285, 664
Bsc4I	CCNNNNNNNGG	9 cut(s)	53, 292, 298, 376, 600, 1086, 1686, 2074, 2209
Bse118I	RCCGGY	1 cut(s)	2299
Bse1I	ACTGG	1 cut(s)	435
BseAI	TCCGGA	2 cut(s)	250, 664
BseBI	CCWGG	7 cut(s)	263, 293, 410, 689, 1234, 1592, 2087
BseCI	ATCGAT	1 cut(s)	1218
BseDI	CCNNGG	6 cut(s)	144, 159, 408, 688, 1232, 1913
BseLI	CCNNNNNNNGG	9 cut(s)	53, 292, 298, 376, 600, 1086, 1686, 2074, 2209
BseMII	CTCAG	3 cut(s)	644, 1130, 1610
BseNI	ACTGG	1 cut(s)	435
BseRI	GAGGAG	4 cut(s)	800, 1335, 1914, 2062
BseSI	GKGCMC	1 cut(s)	491
BseXI	GCAGC	5 cut(s)	520, 1318, 1321, 2094, 2161
BseYI	CCCAGC	2 cut(s)	202, 2057
BsgI	GTGCAG	2 cut(s)	1415, 1944
BshFI	GGCC	7 cut(s)	10, 143, 302, 354, 875, 1595, 1912
BshNI	GGYRCC	3 cut(s)	412, 1587, 2241
BshVI	ATCGAT	1 cut(s)	1218
BsiHKAI	GWGCWC	1 cut(s)	92
BsiSI	CCGG	7 cut(s)	17, 72, 171, 251, 286, 665, 2300
BslFI	GGGAC	1 cut(s)	390
BslI	CCNNNNNNNGG	9 cut(s)	53, 292, 298, 376, 600, 1086, 1686, 2074, 2209
BsmAI	GTCTC	4 cut(s)	32, 1379, 1946, 2124
BsmBI	CGTCTC	2 cut(s)	1379, 1946
BsmFI	GGGAC	1 cut(s)	390
BsmI	GAATGC	3 cut(s)	272, 523, 1117
BsnI	GGCC	7 cut(s)	10, 143, 302, 354, 875, 1595, 1912
Bso31I	GGTCTC	2 cut(s)	32, 2124
Bsp1286I	GDGCHC	2 cut(s)	92, 491
Bsp13I	TCCGGA	2 cut(s)	250, 664
Bsp1720I	GCTNAGC	1 cut(s)	1619
BspACI	CCGC	5 cut(s)	57, 444, 1337, 1634, 2254
BspANI	GGCC	7 cut(s)	10, 143, 302, 354, 875, 1595, 1912
BspCNI	CTCAG	3 cut(s)	643, 1129, 1611
BspDI	ATCGAT	1 cut(s)	1218
BspEI	TCCGGA	2 cut(s)	250, 664
BspMI	ACCTGC	2 cut(s)	648, 1493
BspPI	GGATC	8 cut(s)	74, 283, 296, 336, 998, 1262, 2059, 2072
BspQI	GCTCTTC	1 cut(s)	1495
BspT107I	GGYRCC	3 cut(s)	412, 1587, 2241
BspTNI	GGTCTC	2 cut(s)	32, 2124
BsrBI	CCGCTC	1 cut(s)	444
BsrFI	RCCGGY	1 cut(s)	2299
BsrI	ACTGG	1 cut(s)	435
BssAI	RCCGGY	1 cut(s)	2299
BssECI	CCNNGG	6 cut(s)	144, 159, 408, 688, 1232, 1913
BssSI	CACGAG	1 cut(s)	85
BssT1I	CCWWGG	2 cut(s)	144, 159
Bst2BI	CACGAG	1 cut(s)	85
Bst2UI	CCWGG	7 cut(s)	263, 293, 410, 689, 1234, 1592, 2087
Bst6I	CTCTTC	2 cut(s)	1425, 1495
BstAPI	GCANNNNNTGC	1 cut(s)	1958
BstC8I	GCNNGC	6 cut(s)	911, 973, 1571, 2126, 2301, 2318
BstDEI	CTNAG	4 cut(s)	630, 1049, 1116, 1619
BstEII	GGTNACC	1 cut(s)	2081
BstENI	CCTNNNNNAGG	1 cut(s)	374
BstHHI	GCGC	1 cut(s)	823
BstMAI	GTCTC	4 cut(s)	32, 1379, 1946, 2124
BstMWI	GCNNNNNNNGC	6 cut(s)	503, 654, 1337, 1540, 1958, 2219
BstNI	CCWGG	7 cut(s)	263, 293, 410, 689, 1234, 1592, 2087
BstNSI	RCATGY	2 cut(s)	1687, 2320
BstPAI	GACNNNNGTC	1 cut(s)	1938
BstPI	GGTNACC	1 cut(s)	2081
BstSCI	CCNGG	7 cut(s)	261, 291, 408, 687, 1232, 1590, 2085
BstSFI	CTRYAG	4 cut(s)	537, 1036, 1900, 2142
BstSLI	GKGCMC	1 cut(s)	491
BstV1I	GCAGC	5 cut(s)	520, 1318, 1321, 2094, 2161
BstV2I	GAAGAC	3 cut(s)	27, 248, 1142
BstX2I	RGATCY	6 cut(s)	66, 288, 328, 990, 1773, 2064
BstYI	RGATCY	6 cut(s)	66, 288, 328, 990, 1773, 2064
Bsu15I	ATCGAT	1 cut(s)	1218
BsuI	GTATCC	1 cut(s)	672
BsuRI	GGCC	7 cut(s)	10, 143, 302, 354, 875, 1595, 1912
BsuTUI	ATCGAT	1 cut(s)	1218
BtsIMutI	CAGTG	5 cut(s)	1275, 1526, 1617, 2079, 2277
BveI	ACCTGC	2 cut(s)	648, 1493
Cac8I	GCNNGC	6 cut(s)	911, 973, 1571, 2126, 2301, 2318
CaiI	CAGNNNCTG	1 cut(s)	1958
CfoI	GCGC	1 cut(s)	823
Cfr10I	RCCGGY	1 cut(s)	2299
Cfr13I	GGNCC	4 cut(s)	283, 301, 874, 2307
ClaI	ATCGAT	1 cut(s)	1218
CsiI	ACCWGGT	1 cut(s)	261
Csp6I	GTAC	5 cut(s)	118, 219, 1516, 1840, 1897
CspCI	CAANNNNNGTGG	2 cut(s)	1585, 1620
CviAII	CATG	9 cut(s)	519, 742, 1013, 1316, 1504, 1684, 1837, 1930, 2317
CviQI	GTAC	5 cut(s)	118, 219, 1516, 1840, 1897
DdeI	CTNAG	4 cut(s)	630, 1049, 1116, 1619
DriI	GACNNNNNGTC	2 cut(s)	154, 559
EaeI	YGGCCR	1 cut(s)	352
Eam1104I	CTCTTC	2 cut(s)	1425, 1495
Eam1105I	GACNNNNNGTC	2 cut(s)	154, 559
EarI	CTCTTC	2 cut(s)	1425, 1495
EciI	GGCGGA	1 cut(s)	2243
Eco130I	CCWWGG	2 cut(s)	144, 159
Eco147I	AGGCCT	1 cut(s)	10
Eco31I	GGTCTC	2 cut(s)	32, 2124
Eco47I	GGWCC	2 cut(s)	283, 2307
Eco57I	CTGAAG	3 cut(s)	534, 1702, 2272
Eco91I	GGTNACC	1 cut(s)	2081
EcoNI	CCTNNNNNAGG	1 cut(s)	374
EcoO65I	GGTNACC	1 cut(s)	2081
EcoRII	CCWGG	7 cut(s)	261, 291, 408, 687, 1232, 1590, 2085
EcoT14I	CCWWGG	2 cut(s)	144, 159
EcoT22I	ATGCAT	1 cut(s)	1512
ErhI	CCWWGG	2 cut(s)	144, 159
Esp3I	CGTCTC	2 cut(s)	1379, 1946
FaeI	CATG	9 cut(s)	522, 745, 1016, 1319, 1507, 1687, 1840, 1933, 2320
FaqI	GGGAC	1 cut(s)	390
FatI	CATG	9 cut(s)	518, 741, 1012, 1315, 1503, 1683, 1836, 1929, 2316
FauI	CCCGC	2 cut(s)	50, 437
FauNDI	CATATG	3 cut(s)	984, 1467, 1512
FbaI	TGATCA	2 cut(s)	886, 1768
Fnu4HI	GCNGC	6 cut(s)	534, 1332, 1335, 1338, 2108, 2175
Fsp4HI	GCNGC	6 cut(s)	534, 1332, 1335, 1338, 2108, 2175
FspBI	CTAG	4 cut(s)	456, 906, 998, 2304
GlaI	GCGC	1 cut(s)	822
GluI	GCNGC	6 cut(s)	534, 1332, 1335, 1338, 2108, 2175
GsaI	CCCAGC	2 cut(s)	206, 2061
GsuI	CTGGAG	1 cut(s)	452
HaeIII	GGCC	7 cut(s)	10, 143, 302, 354, 875, 1595, 1912
HapII	CCGG	7 cut(s)	17, 72, 171, 251, 286, 665, 2300
HhaI	GCGC	1 cut(s)	823
Hin1II	CATG	9 cut(s)	522, 745, 1016, 1319, 1507, 1687, 1840, 1933, 2320
Hin6I	GCGC	1 cut(s)	821
HinP1I	GCGC	1 cut(s)	821
HincII	GTYRAC	1 cut(s)	1029
HindII	GTYRAC	1 cut(s)	1029
HindIII	AAGCTT	1 cut(s)	2261
HpaII	CCGG	7 cut(s)	17, 72, 171, 251, 286, 665, 2300
HphI	GGTGA	5 cut(s)	322, 473, 1469, 1474, 2093
Hpy166II	GTNNAC	4 cut(s)	901, 1029, 1402, 1482
Hpy188I	TCNGA	8 cut(s)	384, 529, 775, 1203, 1602, 1773, 2017, 2169
Hpy8I	GTNNAC	4 cut(s)	901, 1029, 1402, 1482
HpyCH4IV	ACGT	2 cut(s)	357, 1731
HpyF10VI	GCNNNNNNNGC	6 cut(s)	503, 654, 1337, 1540, 1958, 2219
HpyF3I	CTNAG	4 cut(s)	630, 1049, 1116, 1619
HpySE526I	ACGT	2 cut(s)	357, 1731
Hsp92II	CATG	9 cut(s)	522, 745, 1016, 1319, 1507, 1687, 1840, 1933, 2320
HspAI	GCGC	1 cut(s)	821
Kpn2I	TCCGGA	2 cut(s)	250, 664
KroI	GCCGGC	1 cut(s)	2299
KroNI	GCCGGC	1 cut(s)	2301
Ksp22I	TGATCA	2 cut(s)	886, 1768
LguI	GCTCTTC	1 cut(s)	1495
LmnI	GCTCC	3 cut(s)	441, 787, 1234
Lsp1109I	GCAGC	5 cut(s)	520, 1318, 1321, 2094, 2161
LweI	GCATC	8 cut(s)	102, 520, 630, 779, 1116, 1153, 1497, 1927
MabI	ACCWGGT	1 cut(s)	261
MaeI	CTAG	4 cut(s)	456, 906, 998, 2304
MaeII	ACGT	2 cut(s)	357, 1731
MaeIII	GTNAC	4 cut(s)	166, 179, 2081, 2191
MbiI	CCGCTC	1 cut(s)	444
MfeI	CAATTG	1 cut(s)	1287
MflI	RGATCY	6 cut(s)	66, 288, 328, 990, 1773, 2064
MhlI	GDGCHC	2 cut(s)	92, 491
MlsI	TGGCCA	1 cut(s)	354
MluNI	TGGCCA	1 cut(s)	354
MlyI	GAGTC	2 cut(s)	1034, 1899
MmeI	TCCRAC	1 cut(s)	1735
Mox20I	TGGCCA	1 cut(s)	354
Mph1103I	ATGCAT	1 cut(s)	1512
MroI	TCCGGA	2 cut(s)	250, 664
MroNI	GCCGGC	1 cut(s)	2299
MroXI	GAANNNNTTC	2 cut(s)	507, 2285
MscI	TGGCCA	1 cut(s)	354
MseI	TTAA	9 cut(s)	198, 615, 758, 800, 963, 1446, 1559, 1745, 2237
MslI	CAYNNNNRTG	6 cut(s)	418, 528, 1314, 1437, 1802, 1968
Msp20I	TGGCCA	1 cut(s)	354
MspI	CCGG	7 cut(s)	17, 72, 171, 251, 286, 665, 2300
MspR9I	CCNGG	7 cut(s)	263, 293, 410, 689, 1234, 1592, 2087
MunI	CAATTG	1 cut(s)	1287
Mva1269I	GAATGC	3 cut(s)	272, 523, 1117
MvaI	CCWGG	7 cut(s)	263, 293, 410, 689, 1234, 1592, 2087
MwoI	GCNNNNNNNGC	6 cut(s)	503, 654, 1337, 1540, 1958, 2219
NaeI	GCCGGC	1 cut(s)	2301
NdeI	CATATG	3 cut(s)	984, 1467, 1512
NgoMIV	GCCGGC	1 cut(s)	2299
NlaIII	CATG	9 cut(s)	522, 745, 1016, 1319, 1507, 1687, 1840, 1933, 2320
NmeAIII	GCCGAG	4 cut(s)	33, 205, 669, 1938
NmuCI	GTSAC	3 cut(s)	166, 179, 2081
NsiI	ATGCAT	1 cut(s)	1512
NspI	RCATGY	2 cut(s)	1687, 2320
OliI	CACNNNNGTG	1 cut(s)	1802
PaeI	GCATGC	1 cut(s)	2320
PaqCI	CACCTGC	1 cut(s)	1493
PceI	AGGCCT	1 cut(s)	10
PciI	ACATGT	1 cut(s)	1683
PciSI	GCTCTTC	1 cut(s)	1495
PctI	GAATGC	3 cut(s)	272, 523, 1117
PdiI	GCCGGC	1 cut(s)	2301
PdmI	GAANNNNTTC	2 cut(s)	507, 2285
PfeI	GAWTC	8 cut(s)	979, 1104, 1346, 1353, 1369, 1955, 2226, 2250
PflMI	CCANNNNNTGG	3 cut(s)	600, 2074, 2209
PkrI	GCNGC	6 cut(s)	535, 1333, 1336, 1339, 2109, 2176
PleI	GAGTC	2 cut(s)	1033, 1898
PpsI	GAGTC	2 cut(s)	1033, 1898
PscI	ACATGT	1 cut(s)	1683
PshAI	GACNNNNGTC	1 cut(s)	1938
PshBI	ATTAAT	1 cut(s)	1745
Psp1406I	AACGTT	1 cut(s)	1731
Psp6I	CCWGG	7 cut(s)	261, 291, 408, 687, 1232, 1590, 2085
PspEI	GGTNACC	1 cut(s)	2081
PspFI	CCCAGC	2 cut(s)	202, 2057
PspGI	CCWGG	7 cut(s)	261, 291, 408, 687, 1232, 1590, 2085
PspPI	GGNCC	4 cut(s)	283, 301, 874, 2307
PstNI	CAGNNNCTG	1 cut(s)	1958
PsuI	RGATCY	6 cut(s)	66, 288, 328, 990, 1773, 2064
RsaI	GTAC	5 cut(s)	119, 220, 1517, 1841, 1898
RsaNI	GTAC	5 cut(s)	118, 219, 1516, 1840, 1897
RseI	CAYNNNNRTG	6 cut(s)	418, 528, 1314, 1437, 1802, 1968
SapI	GCTCTTC	1 cut(s)	1495
SaqAI	TTAA	9 cut(s)	198, 615, 758, 800, 963, 1446, 1559, 1745, 2237
SatI	GCNGC	6 cut(s)	534, 1332, 1335, 1338, 2108, 2175
Sau96I	GGNCC	4 cut(s)	283, 301, 874, 2307
SchI	GAGTC	2 cut(s)	1034, 1899
ScrFI	CCNGG	7 cut(s)	263, 293, 410, 689, 1234, 1592, 2087
SduI	GDGCHC	2 cut(s)	92, 491
SexAI	ACCWGGT	1 cut(s)	261
SfaNI	GCATC	8 cut(s)	102, 520, 630, 779, 1116, 1153, 1497, 1927
SfcI	CTRYAG	4 cut(s)	537, 1036, 1900, 2142
SinI	GGWCC	2 cut(s)	283, 2307
SmiMI	CAYNNNNRTG	6 cut(s)	418, 528, 1314, 1437, 1802, 1968
SmlI	CTYRAG	6 cut(s)	305, 492, 920, 1582, 1639, 2094
SmoI	CTYRAG	6 cut(s)	305, 492, 920, 1582, 1639, 2094
SphI	GCATGC	1 cut(s)	2320
SseBI	AGGCCT	1 cut(s)	10
SsiI	CCGC	5 cut(s)	57, 444, 1337, 1634, 2254
SspMI	CTAG	4 cut(s)	456, 906, 998, 2304
StuI	AGGCCT	1 cut(s)	10
StyD4I	CCNGG	7 cut(s)	261, 291, 408, 687, 1232, 1590, 2085
StyI	CCWWGG	2 cut(s)	144, 159
TaiI	ACGT	2 cut(s)	360, 1734
TaqI	TCGA	5 cut(s)	278, 571, 712, 1218, 1656
TaqII	GACCGA	2 cut(s)	1069, 2324
TatI	WGTACW	1 cut(s)	1515
TauI	GCSGC	1 cut(s)	1340
TfiI	GAWTC	8 cut(s)	979, 1104, 1346, 1353, 1369, 1955, 2226, 2250
Tru1I	TTAA	9 cut(s)	198, 615, 758, 800, 963, 1446, 1559, 1745, 2237
Tru9I	TTAA	9 cut(s)	198, 615, 758, 800, 963, 1446, 1559, 1745, 2237
TscAI	CASTG	5 cut(s)	1282, 1533, 1617, 2079, 2277
TseFI	GTSAC	3 cut(s)	166, 179, 2081
TseI	GCWGC	5 cut(s)	533, 1331, 1334, 2107, 2174
Tsp45I	GTSAC	3 cut(s)	166, 179, 2081
TspRI	CASTG	5 cut(s)	1282, 1533, 1617, 2079, 2277
Van91I	CCANNNNNTGG	3 cut(s)	600, 2074, 2209
VpaK11BI	GGWCC	2 cut(s)	283, 2307
VspI	ATTAAT	1 cut(s)	1745
XagI	CCTNNNNNAGG	1 cut(s)	374
XapI	RAATTY	4 cut(s)	714, 1461, 1985, 2281
XbaI	TCTAGA	1 cut(s)	997
XceI	RCATGY	2 cut(s)	1687, 2320
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2075
XmnI	GAANNNNTTC	2 cut(s)	507, 2285
XspI	CTAG	4 cut(s)	456, 906, 998, 2304
Zsp2I	ATGCAT	1 cut(s)	1512

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.