Rh6DG043200
ERF Family

Belongs to the small GTPase superfamily. Arf family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0001967
Physical Location & Seq
Reverse (-)
4650341 .. 4653464
3124 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG043200.1

Sequence Viewer

Length: 558 bp
ATGTCATCTTTTTCAAGAATGCATCTGAGCCTCTTAGGCCTTTACTTTTGTCAGAAAATGAGTTTCTTTTCAGAATTTGCAATTTTATTTCCACGGTTATTGATTATGTATTTCTTTAAATATCTCACTCAAGAAAGGCACATTCTTTGTCTACAGGTTGGATTCAATATGAGGAAAGTTACAAAAGGGAATGTCACTATAAAGCTGTGGGATCTTGGTGGTCAACCAAGGTTTCGAAGCATGTGGGAAAGATATTGTCGAGCAGTTTCTTCTATTGTTTATGTTGTGGATGCTGCTGATTATGACAACTTACCTGTTTCGAGAAGAGAACTTCATGATCTGTTGAGCAAGCCATCGCTTAGTGGCATTCCATTGCTTGTACTGGGTAACAAGATTGACAAAAAGGAAGCTTTGTCCAAGGATGATTTGACAGAACAAATGGAGCTCAAGTCCATTACTGATAGGGAAGTTTGTTGCTTCATGATTTCTTGCAAGAACTCCACTAACATTGATACCGTCATTGATTGGCTTGTAAAGCATTCAAAAGCCAAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arf

Protein Analysis

185

Amino Acids

21.51

Weight (kDa)

9.14

Isoelectric Point (pI)

42.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
G-alpha PF00503 31 - 133 1.5e-06 G-protein alpha subunit
Gtr1_RagA PF04670 38 - 150 3.3e-06 Gtr1/RagA G protein conserved region
Arf PF00025 52 - 179 3.1e-35 ADP-ribosylation factor family
Ras PF00071 53 - 180 2.8e-10 Ras family
Roc PF08477 61 - 133 2.5e-08 Ras of Complex, Roc, domain of DAPkinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 151
AclWI GGATC 1 cut(s) 219
AcsI RAATTY 1 cut(s) 74
AfaI GTAC 1 cut(s) 381
AgsI TTSAA 3 cut(s) 15, 166, 543
AluBI AGCT 3 cut(s) 205, 410, 445
AluI AGCT 3 cut(s) 205, 410, 445
Alw21I GWGCWC 1 cut(s) 447
AlwI GGATC 1 cut(s) 219
AoxI GGCC 1 cut(s) 37
ApeKI GCWGC 1 cut(s) 293
ApoI RAATTY 1 cut(s) 74
AsuII TTCGAA 1 cut(s) 235
BanII GRGCYC 1 cut(s) 447
Bbv12I GWGCWC 1 cut(s) 447
BbvI GCAGC 1 cut(s) 280
BccI CCATC 1 cut(s) 361
BfmI CTRYAG 1 cut(s) 152
BglI GCCNNNNNGGC 1 cut(s) 36
BisI GCNGC 1 cut(s) 294
BlsI GCNGC 1 cut(s) 295
BmrI ACTGGG 1 cut(s) 392
BmsI GCATC 2 cut(s) 31, 280
BmuI ACTGGG 1 cut(s) 392
Bpu14I TTCGAA 1 cut(s) 235
BpuEI CTTGAG 2 cut(s) 114, 431
BsaJI CCNNGG 3 cut(s) 92, 227, 417
BsaXI ACNNNNNCTCC 2 cut(s) 434, 464
Bse1I ACTGG 1 cut(s) 387
Bse3DI GCAATG 1 cut(s) 371
BseDI CCNNGG 3 cut(s) 92, 227, 417
BseGI GGATG 2 cut(s) 295, 427
BseMI GCAATG 1 cut(s) 371
BseMII CTCAG 1 cut(s) 17
BseNI ACTGG 1 cut(s) 387
BseXI GCAGC 1 cut(s) 280
BshFI GGCC 1 cut(s) 39
BsiHKAI GWGCWC 1 cut(s) 447
BsmI GAATGC 3 cut(s) 24, 366, 538
BsnI GGCC 1 cut(s) 39
Bsp119I TTCGAA 1 cut(s) 235
Bsp1286I GDGCHC 1 cut(s) 447
Bsp143I GATC 2 cut(s) 211, 337
BspANI GGCC 1 cut(s) 39
BspCNI CTCAG 1 cut(s) 18
BspHI TCATGA 2 cut(s) 334, 480
BspPI GGATC 1 cut(s) 219
BspT104I TTCGAA 1 cut(s) 235
BsrDI GCAATG 1 cut(s) 371
BsrI ACTGG 1 cut(s) 387
BssECI CCNNGG 3 cut(s) 92, 227, 417
BssMI GATC 2 cut(s) 211, 337
BssT1I CCWWGG 2 cut(s) 227, 417
Bst4CI ACNGT 2 cut(s) 96, 517
Bst6I CTCTTC 1 cut(s) 319
BstBI TTCGAA 1 cut(s) 235
BstC8I GCNNGC 1 cut(s) 350
BstDEI CTNAG 3 cut(s) 26, 34, 359
BstDSI CCRYGG 1 cut(s) 92
BstF5I GGATG 2 cut(s) 295, 427
BstKTI GATC 2 cut(s) 214, 340
BstMBI GATC 2 cut(s) 211, 337
BstMWI GCNNNNNNNGC 2 cut(s) 36, 535
BstNSI RCATGY 1 cut(s) 244
BstSFI CTRYAG 1 cut(s) 152
BstV1I GCAGC 1 cut(s) 280
BstX2I RGATCY 1 cut(s) 211
BstYI RGATCY 1 cut(s) 211
BsuRI GGCC 1 cut(s) 39
BtgI CCRYGG 1 cut(s) 92
BtgZI GCGATG 1 cut(s) 339
BtsCI GGATG 2 cut(s) 295, 427
Cac8I GCNNGC 1 cut(s) 350
CciI TCATGA 2 cut(s) 334, 480
Csp6I GTAC 1 cut(s) 380
CspCI CAANNNNNGTGG 4 cut(s) 81, 116, 490, 525
CviAII CATG 3 cut(s) 241, 335, 481
CviJI RGCY 8 cut(s) 30, 39, 205, 352, 410, 445, 529, 548
CviKI_1 RGCY 8 cut(s) 30, 39, 205, 352, 410, 445, 529, 548
CviQI GTAC 1 cut(s) 380
DdeI CTNAG 3 cut(s) 26, 34, 359
DpnI GATC 2 cut(s) 213, 339
DpnII GATC 2 cut(s) 211, 337
DraI TTTAAA 1 cut(s) 118
Eam1104I CTCTTC 1 cut(s) 319
EarI CTCTTC 1 cut(s) 319
Ecl136II GAGCTC 1 cut(s) 445
Eco130I CCWWGG 2 cut(s) 227, 417
Eco147I AGGCCT 1 cut(s) 39
Eco24I GRGCYC 1 cut(s) 447
Eco53kI GAGCTC 1 cut(s) 445
EcoICRI GAGCTC 1 cut(s) 445
EcoT14I CCWWGG 2 cut(s) 227, 417
EcoT22I ATGCAT 1 cut(s) 24
EcoT38I GRGCYC 1 cut(s) 447
ErhI CCWWGG 2 cut(s) 227, 417
FaeI CATG 3 cut(s) 244, 338, 484
FaiI YATR 8 cut(s) 107, 170, 200, 242, 282, 303, 336, 482
FatI CATG 3 cut(s) 240, 334, 480
FblI GTMKAC 1 cut(s) 151
Fnu4HI GCNGC 1 cut(s) 294
FokI GGATG 2 cut(s) 302, 434
FriOI GRGCYC 1 cut(s) 447
Fsp4HI GCNGC 1 cut(s) 294
GluI GCNGC 1 cut(s) 294
HaeIII GGCC 1 cut(s) 39
Hin1II CATG 3 cut(s) 244, 338, 484
HincII GTYRAC 1 cut(s) 224
HindII GTYRAC 1 cut(s) 224
HindIII AAGCTT 1 cut(s) 408
HinfI GANTC 1 cut(s) 162
Hpy166II GTNNAC 2 cut(s) 152, 224
Hpy188I TCNGA 3 cut(s) 27, 54, 73
Hpy188III TCNNGA 5 cut(s) 15, 131, 321, 335, 481
Hpy8I GTNNAC 2 cut(s) 152, 224
HpyCH4III ACNGT 2 cut(s) 96, 517
HpyCH4V TGCA 3 cut(s) 22, 80, 492
HpyF10VI GCNNNNNNNGC 2 cut(s) 36, 535
HpyF3I CTNAG 3 cut(s) 26, 34, 359
Hsp92II CATG 3 cut(s) 244, 338, 484
Kzo9I GATC 2 cut(s) 211, 337
LmnI GCTCC 1 cut(s) 442
LpnPI CCDG 3 cut(s) 140, 327, 368
Lsp1109I GCAGC 1 cut(s) 280
LweI GCATC 2 cut(s) 31, 280
MaeIII GTNAC 3 cut(s) 178, 193, 386
MalI GATC 2 cut(s) 213, 339
MboI GATC 2 cut(s) 211, 337
MboII GAAGA 2 cut(s) 261, 336
MflI RGATCY 1 cut(s) 211
MhlI GDGCHC 1 cut(s) 447
MluCI AATT 3 cut(s) 74, 81, 553
MmeI TCCRAC 1 cut(s) 139
MnlI CCTC 2 cut(s) 41, 165
Mph1103I ATGCAT 1 cut(s) 24
MseI TTAA 1 cut(s) 117
Mva1269I GAATGC 3 cut(s) 24, 366, 538
MwoI GCNNNNNNNGC 2 cut(s) 36, 535
NdeII GATC 2 cut(s) 211, 337
NlaIII CATG 3 cut(s) 244, 338, 484
NmuCI GTSAC 1 cut(s) 193
NsiI ATGCAT 1 cut(s) 24
NspI RCATGY 1 cut(s) 244
NspV TTCGAA 1 cut(s) 235
PagI TCATGA 2 cut(s) 334, 480
PceI AGGCCT 1 cut(s) 39
PctI GAATGC 3 cut(s) 24, 366, 538
PfeI GAWTC 1 cut(s) 162
PkrI GCNGC 1 cut(s) 295
Psp124BI GAGCTC 1 cut(s) 447
PsuI RGATCY 1 cut(s) 211
RsaI GTAC 1 cut(s) 381
RsaNI GTAC 1 cut(s) 380
SacI GAGCTC 1 cut(s) 447
SaqAI TTAA 1 cut(s) 117
SatI GCNGC 1 cut(s) 294
Sau3AI GATC 2 cut(s) 211, 337
SduI GDGCHC 1 cut(s) 447
SetI ASST 6 cut(s) 159, 207, 233, 316, 412, 447
SfaNI GCATC 2 cut(s) 31, 280
SfcI CTRYAG 1 cut(s) 152
SfuI TTCGAA 1 cut(s) 235
SmlI CTYRAG 2 cut(s) 129, 446
SmoI CTYRAG 2 cut(s) 129, 446
Sse9I AATT 3 cut(s) 74, 81, 553
SseBI AGGCCT 1 cut(s) 39
SstI GAGCTC 1 cut(s) 447
StuI AGGCCT 1 cut(s) 39
StyI CCWWGG 2 cut(s) 227, 417
TaaI ACNGT 2 cut(s) 96, 517
TaqI TCGA 3 cut(s) 235, 259, 320
TasI AATT 3 cut(s) 74, 81, 553
TatI WGTACW 1 cut(s) 379
TfiI GAWTC 1 cut(s) 162
Tru1I TTAA 1 cut(s) 117
Tru9I TTAA 1 cut(s) 117
TseFI GTSAC 1 cut(s) 193
TseI GCWGC 1 cut(s) 293
Tsp45I GTSAC 1 cut(s) 193
TspDTI ATGAA 2 cut(s) 323, 469
XapI RAATTY 1 cut(s) 74
XceI RCATGY 1 cut(s) 244
XmiI GTMKAC 1 cut(s) 151
Zsp2I ATGCAT 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.