Rroxscaffold_6G00393230
ERF Family

Belongs to the small GTPase superfamily. Arf family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001967
Physical Location & Seq
Forward (+)
13123619 .. 13128539
4921 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00393230.1

Sequence Viewer

Length: 555 bp
ATGGGTTTGTTGGATACCTTCCTCAATTGGCTTCGCAGCTTATTCTTCAAACAGGAAATGGAACTTTCACTAGTCGGGCTTCAGAATGCAGGAAAGACATCTCTTTTAAATGCCATTGCTACAGGAGGGTACAGTGAGGACATGATTCCAACTGTTGGATTCAACATGCGGAAAGTAACCAAGGGAAACGTGACAATTAAGGTTTGGGACCTTGGAGGGCAACGCAGGTTCCGCACAATGTGGGAGCGTTACTGTCGTGGGGTCTCCGTGATTGTGTATGTAGTTGATGCTGCTGACAGGGACAGCGTTCCAATATCTCGAAGTGAGCTACATGACCTCTTGATGAAACCTTCCTTAAATGGAATTCCTTTACTTGTTCTTGGCAACAAAATCGACAAGTCTGAGGCTCTTTCAAAGCAAGCATTGGTTGAACAGCTGGGCCTTGATTCAATCACGGACAAAGAGGTATGTTGCTACATGATCTCATGCAAGGATTCCATAAACATAGATGTTGTGATTGATTGGCTTATCAAACACTCCAAAGCAGCAACCTGA

Protein Analysis

184

Amino Acids

20.56

Weight (kDa)

7.64

Isoelectric Point (pI)

31.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 9 - 178 8.6e-45 ADP-ribosylation factor family
Roc PF08477 22 - 132 4.7e-16 Ras of Complex, Roc, domain of DAPkinase
SRPRB PF09439 22 - 146 5.5e-07 Signal recognition particle receptor beta subunit
Ras PF00071 23 - 179 6.6e-18 Ras family
MMR_HSR1 PF01926 23 - 130 2.3e-07 50S ribosome-binding GTPase
Gtr1_RagA PF04670 24 - 145 7.5e-09 Gtr1/RagA G protein conserved region
G-alpha PF00503 49 - 105 6.1e-06 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 216
AccB7I CCANNNNNTGG 1 cut(s) 155
AciI CCGC 2 cut(s) 169, 232
AcsI RAATTY 1 cut(s) 363
AcuI CTGAAG 1 cut(s) 65
AdeI CACNNNGTG 1 cut(s) 240
AfaI GTAC 1 cut(s) 131
AfiI CCNNNNNNNGG 1 cut(s) 155
AgsI TTSAA 5 cut(s) 49, 163, 414, 431, 450
AhlI ACTAGT 1 cut(s) 70
AluBI AGCT 3 cut(s) 39, 328, 436
AluI AGCT 3 cut(s) 39, 328, 436
Alw26I GTCTC 1 cut(s) 268
AoxI GGCC 1 cut(s) 439
ApeKI GCWGC 3 cut(s) 36, 290, 545
ApoI RAATTY 1 cut(s) 363
AspS9I GGNCC 2 cut(s) 208, 439
AvaII GGWCC 1 cut(s) 208
BbvI GCAGC 2 cut(s) 48, 277
BcgI CGANNNNNNTGC 2 cut(s) 373, 407
BciVI GTATCC 1 cut(s) 7
BcoDI GTCTC 1 cut(s) 268
BcuI ACTAGT 1 cut(s) 70
BfaI CTAG 1 cut(s) 71
BfmI CTRYAG 1 cut(s) 120
BfuAI ACCTGC 1 cut(s) 216
BfuI GTATCC 1 cut(s) 7
BisI GCNGC 3 cut(s) 37, 291, 546
BlsI GCNGC 3 cut(s) 38, 292, 547
Bme18I GGWCC 1 cut(s) 208
BmgT120I GGNCC 2 cut(s) 208, 439
BmiI GGNNCC 2 cut(s) 209, 230
BmsI GCATC 1 cut(s) 277
BsaI GGTCTC 1 cut(s) 268
BsaJI CCNNGG 2 cut(s) 180, 211
Bsc4I CCNNNNNNNGG 1 cut(s) 155
Bse3DI GCAATG 1 cut(s) 114
BseDI CCNNGG 2 cut(s) 180, 211
BseLI CCNNNNNNNGG 1 cut(s) 155
BseMI GCAATG 1 cut(s) 114
BseMII CTCAG 1 cut(s) 393
BseXI GCAGC 2 cut(s) 48, 277
BseYI CCCAGC 1 cut(s) 436
BshFI GGCC 1 cut(s) 441
BslFI GGGAC 2 cut(s) 221, 314
BslI CCNNNNNNNGG 1 cut(s) 155
BsmAI GTCTC 1 cut(s) 268
BsmFI GGGAC 2 cut(s) 221, 314
BsmI GAATGC 1 cut(s) 91
BsnI GGCC 1 cut(s) 441
Bso31I GGTCTC 1 cut(s) 268
Bsp143I GATC 1 cut(s) 480
BspACI CCGC 2 cut(s) 169, 232
BspANI GGCC 1 cut(s) 441
BspCNI CTCAG 1 cut(s) 394
BspLI GGNNCC 2 cut(s) 209, 230
BspMI ACCTGC 1 cut(s) 216
BspTNI GGTCTC 1 cut(s) 268
BsrDI GCAATG 1 cut(s) 114
BssECI CCNNGG 2 cut(s) 180, 211
BssMI GATC 1 cut(s) 480
BssT1I CCWWGG 2 cut(s) 180, 211
Bst4CI ACNGT 3 cut(s) 134, 154, 254
BstC8I GCNNGC 1 cut(s) 420
BstDEI CTNAG 1 cut(s) 402
BstKTI GATC 1 cut(s) 483
BstMAI GTCTC 1 cut(s) 268
BstMBI GATC 1 cut(s) 480
BstMWI GCNNNNNNNGC 1 cut(s) 231
BstNSI RCATGY 1 cut(s) 169
BstSFI CTRYAG 1 cut(s) 120
BstV1I GCAGC 2 cut(s) 48, 277
BsuI GTATCC 1 cut(s) 7
BsuRI GGCC 1 cut(s) 441
BtsIMutI CAGTG 1 cut(s) 139
BveI ACCTGC 1 cut(s) 216
Cac8I GCNNGC 1 cut(s) 420
Cfr13I GGNCC 2 cut(s) 208, 439
Csp6I GTAC 1 cut(s) 130
CviAII CATG 5 cut(s) 142, 166, 332, 478, 486
CviJI RGCY 8 cut(s) 31, 39, 79, 328, 407, 436, 441, 526
CviKI_1 RGCY 8 cut(s) 31, 39, 79, 328, 407, 436, 441, 526
CviQI GTAC 1 cut(s) 130
DdeI CTNAG 1 cut(s) 402
DpnI GATC 1 cut(s) 482
DpnII GATC 1 cut(s) 480
DraI TTTAAA 1 cut(s) 108
DraIII CACNNNGTG 1 cut(s) 240
Eco130I CCWWGG 2 cut(s) 180, 211
Eco31I GGTCTC 1 cut(s) 268
Eco47I GGWCC 1 cut(s) 208
Eco57I CTGAAG 1 cut(s) 65
EcoO109I RGGNCCY 1 cut(s) 208
EcoRI GAATTC 1 cut(s) 363
EcoT14I CCWWGG 2 cut(s) 180, 211
ErhI CCWWGG 2 cut(s) 180, 211
FaeI CATG 5 cut(s) 145, 169, 335, 481, 489
FaiI YATR 9 cut(s) 143, 167, 279, 333, 469, 479, 487, 500, 506
FaqI GGGAC 2 cut(s) 221, 314
FatI CATG 5 cut(s) 141, 165, 331, 477, 485
Fnu4HI GCNGC 3 cut(s) 37, 291, 546
Fsp4HI GCNGC 3 cut(s) 37, 291, 546
FspBI CTAG 1 cut(s) 71
GluI GCNGC 3 cut(s) 37, 291, 546
GsaI CCCAGC 1 cut(s) 440
HaeIII GGCC 1 cut(s) 441
Hin1II CATG 5 cut(s) 145, 169, 335, 481, 489
HinfI GANTC 4 cut(s) 145, 159, 446, 494
Hpy188I TCNGA 2 cut(s) 84, 403
Hpy188III TCNNGA 2 cut(s) 318, 340
HpyAV CCTTC 2 cut(s) 28, 360
HpyCH4III ACNGT 3 cut(s) 134, 154, 254
HpyCH4IV ACGT 1 cut(s) 189
HpyCH4V TGCA 2 cut(s) 89, 489
HpyF10VI GCNNNNNNNGC 1 cut(s) 231
HpyF3I CTNAG 1 cut(s) 402
HpySE526I ACGT 1 cut(s) 189
Hsp92II CATG 5 cut(s) 145, 169, 335, 481, 489
Kzo9I GATC 1 cut(s) 480
LmnI GCTCC 1 cut(s) 244
LpnPI CCDG 6 cut(s) 38, 75, 108, 211, 283, 422
Lsp1109I GCAGC 2 cut(s) 48, 277
LweI GCATC 1 cut(s) 277
MaeI CTAG 1 cut(s) 71
MaeII ACGT 1 cut(s) 189
MaeIII GTNAC 3 cut(s) 175, 190, 248
MalI GATC 1 cut(s) 482
MboI GATC 1 cut(s) 480
MboII GAAGA 1 cut(s) 37
MfeI CAATTG 1 cut(s) 25
MluCI AATT 3 cut(s) 25, 195, 363
MmeI TCCRAC 2 cut(s) 136, 173
MnlI CCTC 7 cut(s) 32, 119, 130, 209, 347, 397, 457
MseI TTAA 3 cut(s) 107, 198, 356
MspA1I CMGCKG 1 cut(s) 436
MunI CAATTG 1 cut(s) 25
Mva1269I GAATGC 1 cut(s) 91
MwoI GCNNNNNNNGC 1 cut(s) 231
NdeII GATC 1 cut(s) 480
NlaIII CATG 5 cut(s) 145, 169, 335, 481, 489
NlaIV GGNNCC 2 cut(s) 209, 230
NmuCI GTSAC 1 cut(s) 190
NspI RCATGY 1 cut(s) 169
PctI GAATGC 1 cut(s) 91
PfeI GAWTC 4 cut(s) 145, 159, 446, 494
PflMI CCANNNNNTGG 1 cut(s) 155
PkrI GCNGC 3 cut(s) 38, 292, 547
PpuMI RGGWCCY 1 cut(s) 208
Psp5II RGGWCCY 1 cut(s) 208
PspFI CCCAGC 1 cut(s) 436
PspN4I GGNNCC 2 cut(s) 209, 230
PspPI GGNCC 2 cut(s) 208, 439
PspPPI RGGWCCY 1 cut(s) 208
PvuII CAGCTG 1 cut(s) 436
RsaI GTAC 1 cut(s) 131
RsaNI GTAC 1 cut(s) 130
SaqAI TTAA 3 cut(s) 107, 198, 356
SatI GCNGC 3 cut(s) 37, 291, 546
Sau3AI GATC 1 cut(s) 480
Sau96I GGNCC 2 cut(s) 208, 439
SfaNI GCATC 1 cut(s) 277
SfcI CTRYAG 1 cut(s) 120
SinI GGWCC 1 cut(s) 208
SpeI ACTAGT 1 cut(s) 70
Sse9I AATT 3 cut(s) 25, 195, 363
SsiI CCGC 2 cut(s) 169, 232
SspMI CTAG 1 cut(s) 71
StyI CCWWGG 2 cut(s) 180, 211
TaaI ACNGT 3 cut(s) 134, 154, 254
TaiI ACGT 1 cut(s) 192
TaqI TCGA 2 cut(s) 319, 393
TasI AATT 3 cut(s) 25, 195, 363
TfiI GAWTC 4 cut(s) 145, 159, 446, 494
Tru1I TTAA 3 cut(s) 107, 198, 356
Tru9I TTAA 3 cut(s) 107, 198, 356
TscAI CASTG 1 cut(s) 139
TseFI GTSAC 1 cut(s) 190
TseI GCWGC 3 cut(s) 36, 290, 545
Tsp45I GTSAC 1 cut(s) 190
TspDTI ATGAA 1 cut(s) 359
TspGWI ACGGA 2 cut(s) 256, 470
TspRI CASTG 1 cut(s) 139
Van91I CCANNNNNTGG 1 cut(s) 155
VpaK11BI GGWCC 1 cut(s) 208
XapI RAATTY 1 cut(s) 363
XceI RCATGY 1 cut(s) 169
XspI CTAG 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.