Rh6BG048800
ERF Family

Belongs to the small GTPase superfamily. Arf family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0001967
Physical Location & Seq
Reverse (-)
8049175 .. 8052418
3244 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG048800.1

Sequence Viewer

Length: 441 bp
ATGGGGTTGTGGGAAGCTTTTCTTAATTGGCTAAGAAGCCTCTTTTTCAAGCAAGAAATGGAGCTATCTTTGATAGGCCTTCAAAATGCCGGGAAAACATCTCTTGTGAATGTTATTGCAACTGGTGGATATAGTGAAGACATGATCCCTACGGTTGGATTCAATATGAGGAAAGTTACAAAAGGGAACGTCACTATAAAGCTGTGGGATCTTGGTGGTCAACCAAGGTTTCGAAGCATGTGGGAAAGATATTGTCGAGCAGTTTCTTCTATTGTTTATGTTGTGGATGCTGCTGATTATGACAACTTACCTGTTTCGAGAAGAGAACTTCATGATCTGTTGAGCAAGCCATCGCTTAGTGGCATTCCATTGCTTGTACTGGGTAACAAGATTGACAAAAAGGAAGCTTTGACCAAGGAAGATTTGACAGAACAAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arf

Protein Analysis

146

Amino Acids

16.53

Weight (kDa)

7.81

Isoelectric Point (pI)

34.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 9 - 145 9.5e-39 ADP-ribosylation factor family
Roc PF08477 22 - 132 1.4e-15 Ras of Complex, Roc, domain of DAPkinase
SRPRB PF09439 22 - 133 6e-06 Signal recognition particle receptor beta subunit
Ras PF00071 23 - 135 1.1e-13 Ras family
MMR_HSR1 PF01926 23 - 130 7.5e-07 50S ribosome-binding GTPase
Gtr1_RagA PF04670 24 - 145 1.3e-09 Gtr1/RagA G protein conserved region
G-alpha PF00503 49 - 106 9.7e-07 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 139, 216
AfaI GTAC 1 cut(s) 378
AfiI CCNNNNNNNGG 1 cut(s) 155
AgsI TTSAA 3 cut(s) 49, 83, 163
AluBI AGCT 4 cut(s) 17, 64, 202, 407
AluI AGCT 4 cut(s) 17, 64, 202, 407
AlwI GGATC 2 cut(s) 139, 216
AoxI GGCC 1 cut(s) 76
ApeKI GCWGC 1 cut(s) 290
Asp700I GAANNNNTTC 1 cut(s) 18
AsuC2I CCSGG 1 cut(s) 91
AsuII TTCGAA 1 cut(s) 232
BbsI GAAGAC 1 cut(s) 144
BbvI GCAGC 1 cut(s) 277
BccI CCATC 1 cut(s) 358
BcnI CCSGG 1 cut(s) 91
BisI GCNGC 1 cut(s) 291
BlsI GCNGC 1 cut(s) 292
Bme1390I CCNGG 1 cut(s) 91
BmrFI CCNGG 1 cut(s) 91
BmrI ACTGGG 1 cut(s) 389
BmsI GCATC 1 cut(s) 277
BmuI ACTGGG 1 cut(s) 389
BpiI GAAGAC 1 cut(s) 144
Bpu14I TTCGAA 1 cut(s) 232
BpuMI CCSGG 1 cut(s) 91
BsaJI CCNNGG 2 cut(s) 224, 414
Bsc4I CCNNNNNNNGG 1 cut(s) 155
Bse1I ACTGG 2 cut(s) 127, 384
Bse3DI GCAATG 1 cut(s) 368
BseDI CCNNGG 2 cut(s) 224, 414
BseGI GGATG 1 cut(s) 292
BseLI CCNNNNNNNGG 1 cut(s) 155
BseMI GCAATG 1 cut(s) 368
BseNI ACTGG 2 cut(s) 127, 384
BseXI GCAGC 1 cut(s) 277
BshFI GGCC 1 cut(s) 78
BsiSI CCGG 1 cut(s) 90
BslI CCNNNNNNNGG 1 cut(s) 155
BsmI GAATGC 1 cut(s) 363
BsnI GGCC 1 cut(s) 78
Bsp119I TTCGAA 1 cut(s) 232
Bsp143I GATC 3 cut(s) 144, 208, 334
BspANI GGCC 1 cut(s) 78
BspHI TCATGA 1 cut(s) 331
BspPI GGATC 2 cut(s) 139, 216
BspT104I TTCGAA 1 cut(s) 232
BsrDI GCAATG 1 cut(s) 368
BsrI ACTGG 2 cut(s) 127, 384
BssECI CCNNGG 2 cut(s) 224, 414
BssMI GATC 3 cut(s) 144, 208, 334
BssT1I CCWWGG 2 cut(s) 224, 414
Bst4CI ACNGT 1 cut(s) 154
Bst6I CTCTTC 1 cut(s) 316
BstBI TTCGAA 1 cut(s) 232
BstC8I GCNNGC 1 cut(s) 347
BstDEI CTNAG 2 cut(s) 32, 356
BstF5I GGATG 1 cut(s) 292
BstKTI GATC 3 cut(s) 147, 211, 337
BstMBI GATC 3 cut(s) 144, 208, 334
BstNSI RCATGY 1 cut(s) 241
BstSCI CCNGG 1 cut(s) 89
BstV1I GCAGC 1 cut(s) 277
BstV2I GAAGAC 1 cut(s) 144
BstX2I RGATCY 1 cut(s) 208
BstYI RGATCY 1 cut(s) 208
BsuRI GGCC 1 cut(s) 78
BtgZI GCGATG 1 cut(s) 336
BtsCI GGATG 1 cut(s) 292
Cac8I GCNNGC 1 cut(s) 347
CciI TCATGA 1 cut(s) 331
Csp6I GTAC 1 cut(s) 377
CviAII CATG 3 cut(s) 142, 238, 332
CviJI RGCY 8 cut(s) 17, 31, 39, 64, 78, 202, 349, 407
CviKI_1 RGCY 8 cut(s) 17, 31, 39, 64, 78, 202, 349, 407
CviQI GTAC 1 cut(s) 377
DdeI CTNAG 2 cut(s) 32, 356
DpnI GATC 3 cut(s) 146, 210, 336
DpnII GATC 3 cut(s) 144, 208, 334
Eam1104I CTCTTC 1 cut(s) 316
EarI CTCTTC 1 cut(s) 316
Eco130I CCWWGG 2 cut(s) 224, 414
Eco147I AGGCCT 1 cut(s) 78
EcoT14I CCWWGG 2 cut(s) 224, 414
ErhI CCWWGG 2 cut(s) 224, 414
FaeI CATG 3 cut(s) 145, 241, 335
FaiI YATR 8 cut(s) 132, 143, 167, 197, 239, 279, 300, 333
FalI AAGNNNNNCTT 1 cut(s) 38
FatI CATG 3 cut(s) 141, 237, 331
Fnu4HI GCNGC 1 cut(s) 291
FokI GGATG 1 cut(s) 299
Fsp4HI GCNGC 1 cut(s) 291
GluI GCNGC 1 cut(s) 291
HaeIII GGCC 1 cut(s) 78
HapII CCGG 1 cut(s) 90
Hin1II CATG 3 cut(s) 145, 241, 335
HincII GTYRAC 1 cut(s) 221
HindII GTYRAC 1 cut(s) 221
HindIII AAGCTT 2 cut(s) 15, 405
HinfI GANTC 1 cut(s) 159
HpaII CCGG 1 cut(s) 90
Hpy166II GTNNAC 1 cut(s) 221
Hpy188III TCNNGA 2 cut(s) 318, 332
Hpy8I GTNNAC 1 cut(s) 221
HpyAV CCTTC 1 cut(s) 89
HpyCH4III ACNGT 1 cut(s) 154
HpyCH4IV ACGT 1 cut(s) 189
HpyCH4V TGCA 1 cut(s) 119
HpyF3I CTNAG 2 cut(s) 32, 356
HpySE526I ACGT 1 cut(s) 189
Hsp92II CATG 3 cut(s) 145, 241, 335
Kzo9I GATC 3 cut(s) 144, 208, 334
LmnI GCTCC 1 cut(s) 61
LpnPI CCDG 4 cut(s) 103, 108, 324, 365
Lsp1109I GCAGC 1 cut(s) 277
LweI GCATC 1 cut(s) 277
MaeII ACGT 1 cut(s) 189
MaeIII GTNAC 3 cut(s) 175, 190, 383
MalI GATC 3 cut(s) 146, 210, 336
MboI GATC 3 cut(s) 144, 208, 334
MboII GAAGA 4 cut(s) 149, 258, 333, 431
MflI RGATCY 1 cut(s) 208
MluCI AATT 1 cut(s) 25
MmeI TCCRAC 1 cut(s) 136
MnlI CCTC 2 cut(s) 50, 162
MroXI GAANNNNTTC 1 cut(s) 18
MseI TTAA 1 cut(s) 24
MspI CCGG 1 cut(s) 90
MspR9I CCNGG 1 cut(s) 91
Mva1269I GAATGC 1 cut(s) 363
NciI CCSGG 1 cut(s) 91
NdeII GATC 3 cut(s) 144, 208, 334
NlaIII CATG 3 cut(s) 145, 241, 335
NmuCI GTSAC 1 cut(s) 190
NspI RCATGY 1 cut(s) 241
NspV TTCGAA 1 cut(s) 232
PagI TCATGA 1 cut(s) 331
PceI AGGCCT 1 cut(s) 78
PctI GAATGC 1 cut(s) 363
PdmI GAANNNNTTC 1 cut(s) 18
PfeI GAWTC 1 cut(s) 159
PkrI GCNGC 1 cut(s) 292
PsuI RGATCY 1 cut(s) 208
RsaI GTAC 1 cut(s) 378
RsaNI GTAC 1 cut(s) 377
SaqAI TTAA 1 cut(s) 24
SatI GCNGC 1 cut(s) 291
Sau3AI GATC 3 cut(s) 144, 208, 334
ScrFI CCNGG 1 cut(s) 91
SetI ASST 7 cut(s) 19, 66, 192, 204, 230, 313, 409
SfaNI GCATC 1 cut(s) 277
SfuI TTCGAA 1 cut(s) 232
Sse9I AATT 1 cut(s) 25
SseBI AGGCCT 1 cut(s) 78
StuI AGGCCT 1 cut(s) 78
StyD4I CCNGG 1 cut(s) 89
StyI CCWWGG 2 cut(s) 224, 414
TaaI ACNGT 1 cut(s) 154
TaiI ACGT 1 cut(s) 192
TaqI TCGA 3 cut(s) 232, 256, 317
TasI AATT 1 cut(s) 25
TatI WGTACW 1 cut(s) 376
TfiI GAWTC 1 cut(s) 159
Tru1I TTAA 1 cut(s) 24
Tru9I TTAA 1 cut(s) 24
TseFI GTSAC 1 cut(s) 190
TseI GCWGC 1 cut(s) 290
Tsp45I GTSAC 1 cut(s) 190
TspDTI ATGAA 1 cut(s) 320
XceI RCATGY 1 cut(s) 241
XmnI GAANNNNTTC 1 cut(s) 18
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.