MD05G1101000.v1.1
ERF Family

Belongs to the small GTPase superfamily. Arf family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001967
Physical Location & Seq
Forward (+)
20954975 .. 20957601
2627 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1101000.v1.1.491

Sequence Viewer

Length: 555 bp
ATGGGGTTGTGGGAAGCTTTTCTCAATTGGCTGCGAAGCCTCTTTTTCAAGCAAGAAATGGAGCTATCCTTAATAGGCCTTCAAAACGCTGGGAAAACATCGCTTGTAAATGTTATTGCAACTGGTGGATATAGTGAAGACATGATCCCGACGGTTGGATTCAATATGAAGAAGGTAACAAAAGGGAATGTCACAATAAAGTTGTGGGATCTTGGTGGTCAGCCAAGGTTTCGAAGCATGTGGGAAAGATATTGCCGTGCAGTTTCCTCTATCGTTTATGTTGTGGATGCTGCTGATTATGATAACTTGCCAGTCTCAAGAAGAGAACTTCATGACTTGTTGAGTAAGTCGTCGCTAAATGGTATTCCACTGCTGGTATTAGGTAACAAGATTGACAAAAAGGAAGCTCTGTCTAAAGAGGATTTGACAGAAGAGATGGATCTGAAGTCCATCACCGACAGAGAAGTTTGTTGCTTCATGATATCCTGCAAGAACTCTACCAACATTGATACGGTCATTGATTGGCTTGTAAAGCATTCGAAGTCAAAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arf

Protein Analysis

185

Amino Acids

20.86

Weight (kDa)

7.63

Isoelectric Point (pI)

32.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 9 - 178 1.7e-46 ADP-ribosylation factor family
Roc PF08477 22 - 132 6.4e-16 Ras of Complex, Roc, domain of DAPkinase
SRPRB PF09439 22 - 142 5e-07 Signal recognition particle receptor beta subunit
Ras PF00071 23 - 179 2.5e-17 Ras family
MMR_HSR1 PF01926 23 - 130 1.3e-06 50S ribosome-binding GTPase
Gtr1_RagA PF04670 24 - 148 1.5e-10 Gtr1/RagA G protein conserved region
G-alpha PF00503 49 - 108 1.4e-06 G-protein alpha subunit
GTP_EFTU PF00009 58 - 180 6.7e-06 Elongation factor Tu GTP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 139, 216, 447
AcuI CTGAAG 1 cut(s) 464
AfiI CCNNNNNNNGG 1 cut(s) 155
AgsI TTSAA 3 cut(s) 49, 83, 163
AluBI AGCT 3 cut(s) 17, 64, 407
AluI AGCT 3 cut(s) 17, 64, 407
Alw26I GTCTC 1 cut(s) 319
AlwI GGATC 3 cut(s) 139, 216, 447
AoxI GGCC 1 cut(s) 76
ApeKI GCWGC 2 cut(s) 31, 290
Asp700I GAANNNNTTC 1 cut(s) 18
AsuHPI GGTGA 1 cut(s) 445
AsuII TTCGAA 2 cut(s) 232, 539
BbsI GAAGAC 1 cut(s) 144
BbvI GCAGC 2 cut(s) 18, 277
BccI CCATC 2 cut(s) 430, 458
BceAI ACGGC 1 cut(s) 240
BcoDI GTCTC 1 cut(s) 319
BisI GCNGC 2 cut(s) 32, 291
BlsI GCNGC 2 cut(s) 33, 292
BmsI GCATC 1 cut(s) 277
BpiI GAAGAC 1 cut(s) 144
Bpu14I TTCGAA 2 cut(s) 232, 539
BpuEI CTTGAG 1 cut(s) 301
BsaJI CCNNGG 1 cut(s) 224
Bsc4I CCNNNNNNNGG 1 cut(s) 155
Bse1I ACTGG 2 cut(s) 127, 311
BseDI CCNNGG 1 cut(s) 224
BseGI GGATG 1 cut(s) 292
BseLI CCNNNNNNNGG 1 cut(s) 155
BseNI ACTGG 2 cut(s) 127, 311
BseXI GCAGC 2 cut(s) 18, 277
BseYI CCCAGC 1 cut(s) 89
BsgI GTGCAG 1 cut(s) 279
BshFI GGCC 1 cut(s) 78
BslI CCNNNNNNNGG 1 cut(s) 155
BsmAI GTCTC 1 cut(s) 319
BsmI GAATGC 1 cut(s) 535
BsnI GGCC 1 cut(s) 78
Bsp119I TTCGAA 2 cut(s) 232, 539
Bsp143I GATC 3 cut(s) 144, 208, 439
BspANI GGCC 1 cut(s) 78
BspHI TCATGA 2 cut(s) 331, 477
BspPI GGATC 3 cut(s) 139, 216, 447
BspT104I TTCGAA 2 cut(s) 232, 539
BsrI ACTGG 2 cut(s) 127, 311
BssECI CCNNGG 1 cut(s) 224
BssMI GATC 3 cut(s) 144, 208, 439
BssT1I CCWWGG 1 cut(s) 224
Bst4CI ACNGT 2 cut(s) 154, 514
Bst6I CTCTTC 2 cut(s) 316, 426
BstBI TTCGAA 2 cut(s) 232, 539
BstF5I GGATG 1 cut(s) 292
BstKTI GATC 3 cut(s) 147, 211, 442
BstMAI GTCTC 1 cut(s) 319
BstMBI GATC 3 cut(s) 144, 208, 439
BstMWI GCNNNNNNNGC 1 cut(s) 532
BstNSI RCATGY 1 cut(s) 241
BstV1I GCAGC 2 cut(s) 18, 277
BstV2I GAAGAC 1 cut(s) 144
BstX2I RGATCY 2 cut(s) 208, 439
BstYI RGATCY 2 cut(s) 208, 439
BsuRI GGCC 1 cut(s) 78
BtgZI GCGATG 1 cut(s) 84
BtsCI GGATG 1 cut(s) 292
BtsI GCAGTG 1 cut(s) 368
BtsIMutI CAGTG 1 cut(s) 368
CciI TCATGA 2 cut(s) 331, 477
CviAII CATG 4 cut(s) 142, 238, 332, 478
CviJI RGCY 8 cut(s) 17, 31, 39, 64, 78, 223, 407, 526
CviKI_1 RGCY 8 cut(s) 17, 31, 39, 64, 78, 223, 407, 526
DpnI GATC 3 cut(s) 146, 210, 441
DpnII GATC 3 cut(s) 144, 208, 439
Eam1104I CTCTTC 2 cut(s) 316, 426
EarI CTCTTC 2 cut(s) 316, 426
Eco130I CCWWGG 1 cut(s) 224
Eco147I AGGCCT 1 cut(s) 78
Eco32I GATATC 1 cut(s) 483
Eco57I CTGAAG 1 cut(s) 464
EcoRV GATATC 1 cut(s) 483
EcoT14I CCWWGG 1 cut(s) 224
ErhI CCWWGG 1 cut(s) 224
FaeI CATG 4 cut(s) 145, 241, 335, 481
FaiI YATR 8 cut(s) 132, 143, 167, 239, 279, 300, 333, 479
FatI CATG 4 cut(s) 141, 237, 331, 477
Fnu4HI GCNGC 2 cut(s) 32, 291
FokI GGATG 1 cut(s) 299
Fsp4HI GCNGC 2 cut(s) 32, 291
GluI GCNGC 2 cut(s) 32, 291
GsaI CCCAGC 1 cut(s) 93
HaeIII GGCC 1 cut(s) 78
Hin1II CATG 4 cut(s) 145, 241, 335, 481
HindIII AAGCTT 1 cut(s) 15
HinfI GANTC 1 cut(s) 159
HphI GGTGA 1 cut(s) 445
Hpy188I TCNGA 1 cut(s) 444
Hpy188III TCNNGA 4 cut(s) 148, 318, 332, 478
Hpy99I CGWCG 2 cut(s) 154, 355
HpyAV CCTTC 2 cut(s) 89, 166
HpyCH4III ACNGT 2 cut(s) 154, 514
HpyCH4V TGCA 3 cut(s) 119, 260, 489
HpyF10VI GCNNNNNNNGC 1 cut(s) 532
Hsp92II CATG 4 cut(s) 145, 241, 335, 481
Kzo9I GATC 3 cut(s) 144, 208, 439
LmnI GCTCC 1 cut(s) 61
LpnPI CCDG 5 cut(s) 75, 108, 324, 359, 499
Lsp1109I GCAGC 2 cut(s) 18, 277
LweI GCATC 1 cut(s) 277
MaeIII GTNAC 3 cut(s) 175, 190, 383
MalI GATC 3 cut(s) 146, 210, 441
MboI GATC 3 cut(s) 144, 208, 439
MboII GAAGA 4 cut(s) 149, 181, 333, 443
MfeI CAATTG 1 cut(s) 25
MflI RGATCY 2 cut(s) 208, 439
MluCI AATT 2 cut(s) 25, 550
MmeI TCCRAC 1 cut(s) 136
MnlI CCTC 3 cut(s) 50, 277, 412
MroXI GAANNNNTTC 1 cut(s) 18
MseI TTAA 1 cut(s) 71
MunI CAATTG 1 cut(s) 25
Mva1269I GAATGC 1 cut(s) 535
MwoI GCNNNNNNNGC 1 cut(s) 532
NdeII GATC 3 cut(s) 144, 208, 439
NlaIII CATG 4 cut(s) 145, 241, 335, 481
NmuCI GTSAC 1 cut(s) 190
NspI RCATGY 1 cut(s) 241
NspV TTCGAA 2 cut(s) 232, 539
PagI TCATGA 2 cut(s) 331, 477
PceI AGGCCT 1 cut(s) 78
PctI GAATGC 1 cut(s) 535
PdmI GAANNNNTTC 1 cut(s) 18
PfeI GAWTC 1 cut(s) 159
PkrI GCNGC 2 cut(s) 33, 292
PspFI CCCAGC 1 cut(s) 89
PsuI RGATCY 2 cut(s) 208, 439
SaqAI TTAA 1 cut(s) 71
SatI GCNGC 2 cut(s) 32, 291
Sau3AI GATC 3 cut(s) 144, 208, 439
SetI ASST 6 cut(s) 19, 66, 177, 230, 385, 409
SfaNI GCATC 1 cut(s) 277
SfuI TTCGAA 2 cut(s) 232, 539
SmlI CTYRAG 1 cut(s) 316
SmoI CTYRAG 1 cut(s) 316
Sse9I AATT 2 cut(s) 25, 550
SseBI AGGCCT 1 cut(s) 78
StuI AGGCCT 1 cut(s) 78
StyI CCWWGG 1 cut(s) 224
TaaI ACNGT 2 cut(s) 154, 514
TaqI TCGA 2 cut(s) 232, 539
TasI AATT 2 cut(s) 25, 550
TfiI GAWTC 1 cut(s) 159
Tru1I TTAA 1 cut(s) 71
Tru9I TTAA 1 cut(s) 71
TscAI CASTG 1 cut(s) 375
TseFI GTSAC 1 cut(s) 190
TseI GCWGC 2 cut(s) 31, 290
Tsp45I GTSAC 1 cut(s) 190
TspDTI ATGAA 3 cut(s) 182, 320, 466
TspRI CASTG 1 cut(s) 375
XceI RCATGY 1 cut(s) 241
XmnI GAANNNNTTC 1 cut(s) 18
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.