FvH4_2g03780
ERF Family

Belongs to the small GTPase superfamily. Arf family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001967
Physical Location & Seq
Reverse (-)
2999637 .. 3002073
2437 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g03780.t1

Sequence Viewer

Length: 555 bp
ATGGGGTTGTGGGAAGCTTTTCTGAATTGGCTACGAAGCCTCTTTTTCAAGCAAGAGATGGAGCTTTCTTTGATAGGCCTTCAAAATGCCGGCAAAACATCACTTGTTAACGTTATTGCAACTGGCGGATATAGTGAAGACATGATCCCTACGGTTGGATTCAATATGAGGAAAGTTACAAAAGGGAGTGTAACTATAAAGCTGTGGGATCTTGGTGGTCAGCCAAGGTTTCGAAGCATGTGGGAAAGATATTGTCGCGCAGTTTCTTCTATTGTTTATGTTGTAGATGCTGCTGATCATGATAACTTGCCTGTTTCAAGAAGAGAACTTCATGACCTGTTGAGCAAGCCATCTCTTAGTGGCATTCCATTGCTGGTACTAGGTAACAAGATTGACAAAAAGGAAGCTTTGTCCAAGGAGGATTTGACAGAACAAATGGAGCTCAAAGCCATTACAGATAGGGAAGTTTGTTGCTTCATGATTTCCTGCAAAAACTCTACTAATATTGATACCGTCATTGATTGGCTTACAAAGCATTCAAAAGCCAAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arf

Protein Analysis

185

Amino Acids

20.8

Weight (kDa)

8.32

Isoelectric Point (pI)

30.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 9 - 178 1.2e-45 ADP-ribosylation factor family
Roc PF08477 22 - 132 3.9e-15 Ras of Complex, Roc, domain of DAPkinase
SRPRB PF09439 22 - 147 2.8e-06 Signal recognition particle receptor beta subunit
Ras PF00071 23 - 179 5.7e-17 Ras family
MMR_HSR1 PF01926 23 - 130 2.3e-06 50S ribosome-binding GTPase
Gtr1_RagA PF04670 24 - 151 1e-09 Gtr1/RagA G protein conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 258
AciI CCGC 1 cut(s) 126
AclI AACGTT 1 cut(s) 111
AclWI GGATC 2 cut(s) 139, 216
AfaI GTAC 1 cut(s) 378
AfiI CCNNNNNNNGG 1 cut(s) 155
AgsI TTSAA 5 cut(s) 49, 83, 163, 318, 540
AluBI AGCT 5 cut(s) 17, 64, 202, 407, 442
AluI AGCT 5 cut(s) 17, 64, 202, 407, 442
Alw21I GWGCWC 1 cut(s) 444
AlwI GGATC 2 cut(s) 139, 216
AoxI GGCC 1 cut(s) 76
ApeKI GCWGC 1 cut(s) 290
Asp700I GAANNNNTTC 1 cut(s) 18
AspLEI GCGC 1 cut(s) 260
AsuII TTCGAA 1 cut(s) 232
BanII GRGCYC 1 cut(s) 444
BbsI GAAGAC 1 cut(s) 144
Bbv12I GWGCWC 1 cut(s) 444
BbvI GCAGC 1 cut(s) 277
BccI CCATC 2 cut(s) 52, 358
BclI TGATCA 1 cut(s) 295
BfaI CTAG 1 cut(s) 380
BisI GCNGC 1 cut(s) 291
BlsI GCNGC 1 cut(s) 292
BmsI GCATC 1 cut(s) 277
BpiI GAAGAC 1 cut(s) 144
Bpu14I TTCGAA 1 cut(s) 232
BsaJI CCNNGG 2 cut(s) 224, 414
Bsc4I CCNNNNNNNGG 1 cut(s) 155
Bse118I RCCGGY 1 cut(s) 89
Bse1I ACTGG 1 cut(s) 127
Bse3DI GCAATG 1 cut(s) 368
BseDI CCNNGG 2 cut(s) 224, 414
BseLI CCNNNNNNNGG 1 cut(s) 155
BseMI GCAATG 1 cut(s) 368
BseNI ACTGG 1 cut(s) 127
BseXI GCAGC 1 cut(s) 277
Bsh1236I CGCG 1 cut(s) 258
BshFI GGCC 1 cut(s) 78
BsiHKAI GWGCWC 1 cut(s) 444
BsiSI CCGG 1 cut(s) 90
BslI CCNNNNNNNGG 1 cut(s) 155
BsmI GAATGC 2 cut(s) 363, 535
BsnI GGCC 1 cut(s) 78
Bsp119I TTCGAA 1 cut(s) 232
Bsp1286I GDGCHC 1 cut(s) 444
Bsp143I GATC 3 cut(s) 144, 208, 295
BspACI CCGC 1 cut(s) 126
BspANI GGCC 1 cut(s) 78
BspFNI CGCG 1 cut(s) 258
BspHI TCATGA 3 cut(s) 298, 331, 477
BspPI GGATC 2 cut(s) 139, 216
BspT104I TTCGAA 1 cut(s) 232
BsrDI GCAATG 1 cut(s) 368
BsrFI RCCGGY 1 cut(s) 89
BsrI ACTGG 1 cut(s) 127
BssAI RCCGGY 1 cut(s) 89
BssECI CCNNGG 2 cut(s) 224, 414
BssMI GATC 3 cut(s) 144, 208, 295
BssT1I CCWWGG 2 cut(s) 224, 414
Bst4CI ACNGT 2 cut(s) 154, 514
Bst6I CTCTTC 1 cut(s) 316
BstBI TTCGAA 1 cut(s) 232
BstC8I GCNNGC 2 cut(s) 91, 347
BstDEI CTNAG 1 cut(s) 356
BstFNI CGCG 1 cut(s) 258
BstHHI GCGC 1 cut(s) 260
BstKTI GATC 3 cut(s) 147, 211, 298
BstMBI GATC 3 cut(s) 144, 208, 295
BstMWI GCNNNNNNNGC 1 cut(s) 532
BstNSI RCATGY 1 cut(s) 241
BstUI CGCG 1 cut(s) 258
BstV1I GCAGC 1 cut(s) 277
BstV2I GAAGAC 1 cut(s) 144
BstX2I RGATCY 1 cut(s) 208
BstYI RGATCY 1 cut(s) 208
BsuRI GGCC 1 cut(s) 78
Cac8I GCNNGC 2 cut(s) 91, 347
CciI TCATGA 3 cut(s) 298, 331, 477
CfoI GCGC 1 cut(s) 260
Cfr10I RCCGGY 1 cut(s) 89
Csp6I GTAC 1 cut(s) 377
CviAII CATG 5 cut(s) 142, 238, 299, 332, 478
CviQI GTAC 1 cut(s) 377
DdeI CTNAG 1 cut(s) 356
DpnI GATC 3 cut(s) 146, 210, 297
DpnII GATC 3 cut(s) 144, 208, 295
Eam1104I CTCTTC 1 cut(s) 316
EarI CTCTTC 1 cut(s) 316
EciI GGCGGA 1 cut(s) 141
Ecl136II GAGCTC 1 cut(s) 442
Eco130I CCWWGG 2 cut(s) 224, 414
Eco147I AGGCCT 1 cut(s) 78
Eco24I GRGCYC 1 cut(s) 444
Eco53kI GAGCTC 1 cut(s) 442
EcoICRI GAGCTC 1 cut(s) 442
EcoT14I CCWWGG 2 cut(s) 224, 414
EcoT38I GRGCYC 1 cut(s) 444
ErhI CCWWGG 2 cut(s) 224, 414
FaeI CATG 5 cut(s) 145, 241, 302, 335, 481
FaiI YATR 9 cut(s) 132, 143, 167, 197, 239, 279, 300, 333, 479
FatI CATG 5 cut(s) 141, 237, 298, 331, 477
FbaI TGATCA 1 cut(s) 295
Fnu4HI GCNGC 1 cut(s) 291
FriOI GRGCYC 1 cut(s) 444
Fsp4HI GCNGC 1 cut(s) 291
FspBI CTAG 1 cut(s) 380
GlaI GCGC 1 cut(s) 259
GluI GCNGC 1 cut(s) 291
HaeIII GGCC 1 cut(s) 78
HapII CCGG 1 cut(s) 90
HhaI GCGC 1 cut(s) 260
Hin1II CATG 5 cut(s) 145, 241, 302, 335, 481
Hin6I GCGC 1 cut(s) 258
HinP1I GCGC 1 cut(s) 258
HincII GTYRAC 1 cut(s) 109
HindII GTYRAC 1 cut(s) 109
HindIII AAGCTT 2 cut(s) 15, 405
HinfI GANTC 1 cut(s) 159
HpaI GTTAAC 1 cut(s) 109
HpaII CCGG 1 cut(s) 90
Hpy166II GTNNAC 1 cut(s) 109
Hpy188I TCNGA 1 cut(s) 24
Hpy188III TCNNGA 4 cut(s) 299, 318, 332, 478
Hpy8I GTNNAC 1 cut(s) 109
HpyAV CCTTC 1 cut(s) 89
HpyCH4III ACNGT 2 cut(s) 154, 514
HpyCH4IV ACGT 1 cut(s) 111
HpyCH4V TGCA 2 cut(s) 119, 489
HpyF10VI GCNNNNNNNGC 1 cut(s) 532
HpyF3I CTNAG 1 cut(s) 356
HpySE526I ACGT 1 cut(s) 111
Hsp92II CATG 5 cut(s) 145, 241, 302, 335, 481
HspAI GCGC 1 cut(s) 258
KroI GCCGGC 1 cut(s) 89
KroNI GCCGGC 1 cut(s) 91
Ksp22I TGATCA 1 cut(s) 295
KspAI GTTAAC 1 cut(s) 109
Kzo9I GATC 3 cut(s) 144, 208, 295
LmnI GCTCC 2 cut(s) 61, 439
LpnPI CCDG 6 cut(s) 103, 108, 324, 350, 359, 499
Lsp1109I GCAGC 1 cut(s) 277
LweI GCATC 1 cut(s) 277
MaeI CTAG 1 cut(s) 380
MaeII ACGT 1 cut(s) 111
MaeIII GTNAC 3 cut(s) 175, 190, 383
MalI GATC 3 cut(s) 146, 210, 297
MboI GATC 3 cut(s) 144, 208, 295
MboII GAAGA 3 cut(s) 149, 258, 333
MflI RGATCY 1 cut(s) 208
MhlI GDGCHC 1 cut(s) 444
MluCI AATT 2 cut(s) 25, 550
MmeI TCCRAC 1 cut(s) 136
MnlI CCTC 3 cut(s) 50, 162, 412
MroNI GCCGGC 1 cut(s) 89
MroXI GAANNNNTTC 1 cut(s) 18
MseI TTAA 1 cut(s) 108
MspI CCGG 1 cut(s) 90
Mva1269I GAATGC 2 cut(s) 363, 535
MvnI CGCG 1 cut(s) 258
MwoI GCNNNNNNNGC 1 cut(s) 532
NaeI GCCGGC 1 cut(s) 91
NdeII GATC 3 cut(s) 144, 208, 295
NgoMIV GCCGGC 1 cut(s) 89
NlaIII CATG 5 cut(s) 145, 241, 302, 335, 481
NspI RCATGY 1 cut(s) 241
NspV TTCGAA 1 cut(s) 232
PagI TCATGA 3 cut(s) 298, 331, 477
PceI AGGCCT 1 cut(s) 78
PctI GAATGC 2 cut(s) 363, 535
PdiI GCCGGC 1 cut(s) 91
PdmI GAANNNNTTC 1 cut(s) 18
PfeI GAWTC 1 cut(s) 159
PkrI GCNGC 1 cut(s) 292
Psp124BI GAGCTC 1 cut(s) 444
Psp1406I AACGTT 1 cut(s) 111
PsuI RGATCY 1 cut(s) 208
RsaI GTAC 1 cut(s) 378
RsaNI GTAC 1 cut(s) 377
SacI GAGCTC 1 cut(s) 444
SaqAI TTAA 1 cut(s) 108
SatI GCNGC 1 cut(s) 291
Sau3AI GATC 3 cut(s) 144, 208, 295
SduI GDGCHC 1 cut(s) 444
SetI ASST 9 cut(s) 19, 66, 114, 204, 230, 339, 385, 409, 444
SfaNI GCATC 1 cut(s) 277
SfuI TTCGAA 1 cut(s) 232
Sse9I AATT 2 cut(s) 25, 550
SseBI AGGCCT 1 cut(s) 78
SsiI CCGC 1 cut(s) 126
SspI AATATT 1 cut(s) 505
SspMI CTAG 1 cut(s) 380
SstI GAGCTC 1 cut(s) 444
StuI AGGCCT 1 cut(s) 78
StyI CCWWGG 2 cut(s) 224, 414
TaaI ACNGT 2 cut(s) 154, 514
TaiI ACGT 1 cut(s) 114
TaqI TCGA 1 cut(s) 232
TasI AATT 2 cut(s) 25, 550
TfiI GAWTC 1 cut(s) 159
Tru1I TTAA 1 cut(s) 108
Tru9I TTAA 1 cut(s) 108
TseI GCWGC 1 cut(s) 290
TspDTI ATGAA 2 cut(s) 320, 466
XceI RCATGY 1 cut(s) 241
XmnI GAANNNNTTC 1 cut(s) 18
XspI CTAG 1 cut(s) 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.