AT1G66730

DNA ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0015031
Physical Location & Seq
Forward (+)
24884681 .. 24892046
7366 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G66730.1

Sequence Viewer

Length: 4191 bp
ATGGCCTCTGATTCCGCCGGAGCCACAATCTCCGGCAACTTCTCAAACTCCGACAACTCAGAAACCCTAAATCTCAACACTACGAAACTCTACTCATCAGCAATTTCCTCTATCTCTCCACAATTCCCCTCACCGAAACCAACATCTTCATGTCCTTCAATCCCTAATTCAAAACGAATCCCTAATACTAACTTCATCGTCGATCTCTTCCGCCTCCCACACCAATCATCCTCCGTCGCTTTCTTCCTCTCACATTTCCACTCCGATCACTACTCCGGTCTCTCCTCTTCCTGGTCCAAAGGAATAATCTACTGCTCACACAAAACCGCTCGTCTCGTTGCAGAAATCCTTCAAGTTCCTTCACAATTCGTTTTCGCTTTACCTATGAACCAAATGGTTAAGATCGATGGCTCTGAAGTTGTGTTGATTGAAGCTAATCATTGTCCAGGAGCGGTACAGTTTTTGTTTAAAGTCAAATTAGAGAGCAGTGGATTTGAAAAGTATGTTCATACTGGAGATTTTAGATTCTGTGATGAGATGAGATTTGATCCGTTTCTTAATGGTTTTGTTGGTTGTGATGGTGTTTTTCTCGATACTACTTATTGTAATCCTAAGTTTGTTTTTCCGAGTCAAGAAGAGTCTGTTGGTTATGTTGTTAGTGTGATAGATAAGATTAGTGAGGAGAAGGTTTTGTTTCTTGTAGCTACTTATGTTGTTGGTAAAGAGAAGATTTTGGTTGAGATTGCTAGGAGATGTAAGAGGAAAATCGTTGTCGATGCGAGAAAGATGTCGATGTTGTCGGTTTTGGGGTGTGGTGAGGAGGGAATGTTTACTGAAGATGAGAATGAGAGTGATGTTCATGTTGTGGGGTGGAATGTGTTGGGTGAGACTTGGCCGTATTTTAGGCCGAATTTTGTGAAGATGAATGAGATTATGGTTGAGAAAGGGTATGATAAGGTTGTAGGTTTTGTGCCTACAGGGTGGACTTATGAAGTGAAGCGGAATAAGTTTGCGGTTAGGTTTAAAGATTCGATGGAGATTCATCTTGTACCGTATAGTGAGCATTCGAATTATGATGAACTTAGAGAGTTTATAAAGTTCCTGAAACCTAAAAGAGTTATTCCTACTGTTGGAGTTGATATTGAGAAGTTTGATTGCAAGGAGGTTAATAAGATGCAGAAGCATTTCTCTGGATTGGTTGATGAGATGGCAAACAAGAAAGATTTTTTATTGGGTTTCTATCGTCAGTCTTACCAGAAGAATGAGAAAAGTGATGTAGATGTTGTGTCACACAGTGCTGAGGTTTACGAAGAGGAAGAGAAAAATGCATGTGAAGACGGTGGAGAGAATGTTCCATCATCTAGAGGACCCATATTGCATGATACAACTCCAAGTAGTGATTCAAGATTGCTGATAAAACTACGTGATTCTTTGCCTGCTTGGGTCACTGAAGAGCAGATGTTAGATCTGATTAAGAAGCATGCTGGTAATCCCGTGGATATAGTAAGTAACTTTTATGAATATGAAGCAGAGCTTTACAAGCAAGCCTCTCTTCCCACGCCTTCCTTGAATAATCAGGCTGTTTTGTTTGATGACGATGTAACCGACCTCCAACCTAATCCTGTGAAAGGCATTTGTCCAGATGTTCAAGCAATTCAAAAAGGTTTTGACTTACCAAGAAAAATGAATTTAACAAAGGGTACTATTTCTCCTGGGAAGAGAGGCAAAAGCTCGGGCAGTAAGTCAAACAAGAAGGCCAAGAAAGATCCAAAATCAAAACCAGTTGGTCCAGGGCAGCCAACACTTTTTAAGTTTTTCAATAAGGTCTTAGATGGTGGATCTAATTCTGTTAGTGTTGGTTCAGAGACTGAAGAATGCAATACAGATAAAAAGATGGTCCACATTGATGCTTCAGAAGCATACAAAGAAGTGACAGATCAATTTATTGATATTGTTAACGGTTCTGAATCTTTAAGAGATTATGCTGCTTCCATCATTGATGAGGCTAAAGGAGATATAAGTAGGGCACTGAACATCTATTATAGCAAACCCCGTGAAATACCTGGTGACCATGCAGGTGAGAGAGGACTCTCTAGCAAAACAATTCAGTATCCGAAATGTTCAGAAGCATGCTCCTCTCAGGAGGACAAAAAGGCTTCAGAAAATTCAGGACATGCAGTCAACATATGCGTGCAGACATCAGCAGAGGAAAGTGTAGACAAGAATTATGTATCTCTACCTCCTGAAAAATATCAACCTAAAGAGCACGCTTGTTGGAGGGAGGGACAGCCTGCTCCATATATCCACCTTGTCCGAACATTTGCTTCTGTTGAAAGTGAAAAGGGAAAGATCAAAGCTATGTCTATGCTTTGCAACATGTTTAGAAGCTTGTTTGCTCTCTCTCCAGAAGATGTTCTGCCCGCTGTTTATCTTTGCACAAATAAGATTGCCGCTGACCATGAGAATATTGAGCTTAACATTGGTGGAAGTCTAATCTCTTCTGCATTGGAAGAAGCATGTGGAATAAGTCGGTCTACTGTGAGAGATATGTATAATAGCTTGGGGGATCTTGGGGATGTTGCTCAGCTATGTCGACAAACGCAAAAGCTACTGGTTCCTCCACCTCCACTTCTAGTTAGAGATGTGTTTTCAACTCTACGAAAGATAAGTGTGCAGACAGGTACTGGAAGTACTAGGCTAAAGAAAAATCTCATCGTGAAGCTAATGCGCTCTTGTAGAGAGAAAGAGATCAAATTTCTTGTTAGAACATTGGCCCGTAATTTAAGGATTGGCGCTATGTTGAGAACTGTTCTACCTGCACTTGGGCGAGCAATCGTTATGAATTCTTTCTGGAATGACCATAATAAAGAACTATCAGAAAGCTGTTTCAGGGAGAAACTTGAGGGTGTTTCTGCTGCAGTAGTTGAGGCTTACAACATTCTTCCATCTCTGGACGTGGTTGTTCCTTCTCTCATGGACAAAGACATTGAGTTTTCAACGTCAACTCTGTCAATGGTCCCAGGGATACCTATAAAACCCATGCTTGCAAAAATTGCTAAGGGGGTTCAAGAATTTTTTAACCTCTCCCAAGAAAAGGCCTTTACTTGTGAATACAAATATGATGGCCAGCGCGCCCAGATCCATAAGTTACTTGATGGTACTGTGTGTATCTTTTCTCGGAATGGGGACGAAACAACGTCCAGATTTCCGGATCTGGTTGATGTCATAAAACAGTTTTCTTGCCCTGCCGCTGAGACATTCATGTTGGACGCAGAGGTGGTTGCCACTGATAGAATAAATGGAAACAAACTCATGTCATTCCAAGAGTTATCAACAAGAGAGAGAGGGAGCAAAGATGCTTTGATAACTACAGAAAGTATTAAGGTTGAAGTCTGCGTCTTCGTGTTTGATATTATGTTTGTCAATGGAGAACAGCTGTTGGCTCTTCCCCTCCGTGAGAGACGAAGACGTCTAAAGGAGGTCTTCCCTGAGACTAGGCCAGGTTATCTTGAGTATGCTAAGGAAATAACTGTGGGAGCAGAAGAAGCGTCTCTGAATAATCATGACACATTGAGTAGGATAAATGCTTTTCTTGAAGAAGCATTTCAGTCATCTTGTGAAGGAATCATGGTCAAGTCTTTAGATGTAAATGCTGGATACTGCCCCACAAAGCGCTCTGATTCATGGCTCAAGGTTAAGCGAGATTATGTAGATGGATTGGGCGATACATTGGATTTAGTTCCTATTGGTGCTTGGTATGGCAATGGGAGAAAAGCAGGATGGTATAGTCCATTCCTTATGGCCTGCTTCAACCCTGAGACTGAAGAATTCCAGAGTGTCTGCCGTGTCATGTCTGGTTTTTCTGATGCCTTTTACATCGAGATGAAAGAATTCTACTCGGAGGATAAGATCCTTGCGAAAAAGCCGCCTTACTACAGAACAGGGGAGACACCTGACATGTGGTTTTCCGCAGAGGTAGTTTGGGAAATCAGAGGTGCTGATTTCACAGTCTCGCCTGTTCACAGTGCCAGTTTAGGTCTCGTCCATCCATCGCGAGGCATCTCTGTTAGGTTTCCGAGATTTATCAGTAAAGTGACAGATAGAAATCCTGAAGAATGTAGTACGGCTACAGATATCGCAGAGATGTTTCATGCTCAAACCAGAAAAATGAACATCACTTCTCAACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000012 GO:0000726 GO:0002237 GO:0003006 GO:0003674 GO:0003824 GO:0003909 GO:0003910 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006266 GO:0006271 GO:0006273 GO:0006281 GO:0006302 GO:0006303 GO:0006304 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009314 GO:0009409 GO:0009411 GO:0009416 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009791 GO:0009845 GO:0009987 GO:0010033 GO:0010154 GO:0010225 GO:0010243 GO:0015074 GO:0016070 GO:0016874 GO:0016886 GO:0022414 GO:0022616 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0033554 GO:0034641 GO:0034645 GO:0035510 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044728 GO:0046483 GO:0046677 GO:0048316 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051103 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0065007 GO:0070013 GO:0070988 GO:0071704 GO:0080111 GO:0090304 GO:0090351 GO:0140097 GO:1901360 GO:1901576 GO:1901652 GO:1901698 GO:1901700 GO:1904975 GO:2000683 GO:2000685
KEGG Pathways
Metabolic & Signaling
ko03030 ko03410 ko03420 ko03430 map03030 map03410 map03420 map03430
Pfam Domains
Protein Families
DNA_ligase_A_C DNA_ligase_A_M DNA_ligase_A_N DRMBL

Protein Analysis

1396

Amino Acids

156.32

Weight (kDa)

5.95

Isoelectric Point (pI)

47.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DRMBL PF07522 274 - 379 2.3e-21 DNA repair metallo-beta-lactamase
DNA_ligase_A_N PF04675 766 - 941 1.7e-41 DNA ligase N terminus
DNA_ligase_A_M PF01068 1013 - 1233 1.1e-50 ATP dependent DNA ligase domain
DNA_ligase_A_C PF04679 1259 - 1363 1.9e-14 ATP dependent DNA ligase C terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015031)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66730
fragaria_vesca FvH4_1g29000 FvH4_1g29000 FvH4_1g29000
malus_domestica MD00G1054100.v1.1
prunus_persica Prupe.3G150300_v2.0.a1
pyrus_communis pycom17g25560
rosa_chinensis RchiOBHm_Chr3g0493691 RchiOBHm_Chr3g0493951
rosa_laevigata RLG00000022694
rosa_roxburghii Rroxscaffold_6G00391370
rosa_rugosa Rorug03G0263400 Rorug03G0263500 Rorug03G0263600
rosa_samantha Rh3AG312500 Rh3AG313200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1094
AarI CACCTGC 1 cut(s) 2066
AasI GACNNNNNNGTC 1 cut(s) 3468
AatII GACGTC 1 cut(s) 3472
Acc36I ACCTGC 2 cut(s) 2066, 2824
AccBSI CCGCTC 2 cut(s) 329, 452
AccI GTMKAC 3 cut(s) 2217, 2534, 2593
AccII CGCG 2 cut(s) 3132, 4054
AccIII TCCGGA 1 cut(s) 3208
AclWI GGATC 7 cut(s) 542, 1760, 1846, 2574, 3133, 3219, 3904
AcoI YGGCCR 2 cut(s) 893, 3124
AcsI RAATTY 8 cut(s) 910, 1687, 2164, 2753, 2842, 3071, 3827, 3890
AcuI CTGAAG 8 cut(s) 435, 855, 1470, 1890, 1896, 2142, 3843, 4131
AcyI GRCGYC 1 cut(s) 3469
AdeI CACNNNGTG 1 cut(s) 1295
AfaI GTAC 7 cut(s) 456, 1050, 1702, 2683, 2692, 3160, 4123
AfeI AGCGCT 1 cut(s) 3674
AfiI CCNNNNNNNGG 6 cut(s) 291, 1130, 1628, 2822, 3094, 4055
AflIII ACRYGT 2 cut(s) 2376, 3957
AhdI GACNNNNNGTC 1 cut(s) 2177
AjiI CACGTC 1 cut(s) 2956
AjnI CCWGG 7 cut(s) 290, 445, 1711, 1789, 2062, 3019, 3499
AjuI GAANNNNNNNTTGG 4 cut(s) 627, 659, 1384, 1416
AloI GAACNNNNNNTCC 2 cut(s) 1335, 1367
Alw21I GWGCWC 1 cut(s) 2268
AlwI GGATC 7 cut(s) 542, 1760, 1846, 2574, 3133, 3219, 3904
AlwNI CAGNNNCTG 2 cut(s) 1868, 2684
Ama87I CYCGRG 1 cut(s) 1732
Aor13HI TCCGGA 1 cut(s) 3208
Aor51HI AGCGCT 1 cut(s) 3674
AoxI GGCC 9 cut(s) 3, 893, 905, 1755, 2772, 3096, 3124, 3497, 3801
ApeKI GCWGC 3 cut(s) 1795, 1985, 2915
ApoI RAATTY 8 cut(s) 910, 1687, 2164, 2753, 2842, 3071, 3827, 3890
Asp700I GAANNNNTTC 6 cut(s) 348, 1184, 2412, 2846, 3603, 3890
AspLEI GCGC 5 cut(s) 2730, 2795, 3132, 3134, 3675
AspS9I GGNCC 6 cut(s) 294, 1367, 1787, 1897, 2773, 3016
AsuHPI GGTGA 5 cut(s) 123, 827, 896, 2078, 2090
AsuII TTCGAA 1 cut(s) 1067
AvaI CYCGRG 1 cut(s) 1732
AvaII GGWCC 5 cut(s) 294, 1367, 1787, 1897, 3016
BaeGI GKGCMC 1 cut(s) 2029
BalI TGGCCA 1 cut(s) 3126
BbsI GAAGAC 4 cut(s) 1341, 3391, 3472, 3475
Bbv12I GWGCWC 1 cut(s) 2268
BbvCI CCTCAGC 1 cut(s) 1299
BbvI GCAGC 3 cut(s) 1807, 1972, 2902
BceAI ACGGC 3 cut(s) 880, 3828, 4140
BciT130I CCWGG 7 cut(s) 292, 447, 1713, 1791, 2064, 3021, 3501
BciVI GTATCC 3 cut(s) 2121, 3018, 3650
BfaI CTAG 6 cut(s) 747, 1362, 2094, 2633, 2694, 3495
BfmI CTRYAG 5 cut(s) 975, 2916, 3369, 3934, 4128
BfoI RGCGCY 2 cut(s) 2796, 3676
BfuAI ACCTGC 2 cut(s) 2066, 2824
BfuI GTATCC 3 cut(s) 2121, 3018, 3650
BglII AGATCT 1 cut(s) 1465
BisI GCNGC 6 cut(s) 1796, 1986, 2451, 2916, 3249, 3926
BlpI GCTNAGC 1 cut(s) 2583
BlsI GCNGC 6 cut(s) 1797, 1987, 2452, 2917, 3250, 3927
BmcAI AGTACT 1 cut(s) 2692
Bme1390I CCNGG 7 cut(s) 292, 447, 1713, 1791, 2064, 3021, 3501
Bme18I GGWCC 5 cut(s) 294, 1367, 1787, 1897, 3016
BmeRI GACNNNNNGTC 1 cut(s) 2177
BmeT110I CYCGRG 1 cut(s) 1732
BmgBI CACGTC 1 cut(s) 2956
BmgT120I GGNCC 6 cut(s) 294, 1367, 1787, 1897, 2773, 3016
BmiI GGNNCC 4 cut(s) 22, 1369, 2616, 3018
BmrFI CCNGG 7 cut(s) 292, 447, 1713, 1791, 2064, 3021, 3501
BmsI GCATC 6 cut(s) 766, 1164, 1897, 3346, 3856, 4068
BpiI GAAGAC 4 cut(s) 1341, 3391, 3472, 3475
BpmI CTGGAG 2 cut(s) 534, 2388
Bpu10I CCTNAGC 3 cut(s) 1299, 3057, 3519
Bpu1102I GCTNAGC 1 cut(s) 2583
Bpu14I TTCGAA 1 cut(s) 1067
BpuEI CTTGAG 3 cut(s) 2921, 3530, 3674
Bsa29I ATCGAT 1 cut(s) 405
BsaAI YACGTR 1 cut(s) 1424
BsaBI GATNNNNATC 1 cut(s) 4104
BsaHI GRCGYC 1 cut(s) 3469
BsaI GGTCTC 2 cut(s) 284, 4043
BsaJI CCNNGG 5 cut(s) 1494, 1712, 1790, 3019, 3020
BsaWI WCCGGW 2 cut(s) 275, 3208
BsaXI ACNNNNNCTCC 6 cut(s) 812, 842, 1335, 1365, 1693, 1723
Bsc4I CCNNNNNNNGG 6 cut(s) 291, 1130, 1628, 2822, 3094, 4055
Bse1I ACTGG 5 cut(s) 517, 1781, 2616, 2689, 4029
Bse3DI GCAATG 1 cut(s) 3769
Bse8I GATNNNNATC 1 cut(s) 4104
BseAI TCCGGA 1 cut(s) 3208
BseBI CCWGG 7 cut(s) 292, 447, 1713, 1791, 2064, 3021, 3501
BseCI ATCGAT 1 cut(s) 405
BseDI CCNNGG 5 cut(s) 1494, 1712, 1790, 3019, 3020
BseGI GGATG 4 cut(s) 227, 2581, 3785, 4045
BseJI GATNNNNATC 1 cut(s) 4104
BseLI CCNNNNNNNGG 6 cut(s) 291, 1130, 1628, 2822, 3094, 4055
BseMI GCAATG 1 cut(s) 3769
BseMII CTCAG 7 cut(s) 72, 1290, 2153, 2597, 3243, 3480, 3807
BseNI ACTGG 5 cut(s) 517, 1781, 2616, 2689, 4029
BsePI GCGCGC 1 cut(s) 3130
BseRI GAGGAG 4 cut(s) 274, 695, 833, 2125
BseSI GKGCMC 1 cut(s) 2029
BseXI GCAGC 3 cut(s) 1807, 1972, 2902
BsgI GTGCAG 3 cut(s) 2213, 2693, 2802
Bsh1236I CGCG 2 cut(s) 3132, 4054
BshFI GGCC 9 cut(s) 5, 895, 907, 1757, 2774, 3098, 3126, 3499, 3803
BshVI ATCGAT 1 cut(s) 405
BsiHKAI GWGCWC 1 cut(s) 2268
BsiHKCI CYCGRG 1 cut(s) 1732
BsiSI CCGG 4 cut(s) 18, 33, 276, 3209
BslFI GGGAC 3 cut(s) 2298, 3002, 3200
BslI CCNNNNNNNGG 6 cut(s) 291, 1130, 1628, 2822, 3094, 4055
BsmBI CGTCTC 3 cut(s) 338, 3454, 3555
BsmFI GGGAC 3 cut(s) 2298, 3002, 3200
BsmI GAATGC 2 cut(s) 1063, 1880
BsnI GGCC 9 cut(s) 5, 895, 907, 1757, 2774, 3098, 3126, 3499, 3803
Bso31I GGTCTC 2 cut(s) 284, 4043
BsoBI CYCGRG 1 cut(s) 1732
Bsp119I TTCGAA 1 cut(s) 1067
Bsp1286I GDGCHC 2 cut(s) 2029, 2268
Bsp13I TCCGGA 1 cut(s) 3208
Bsp1720I GCTNAGC 1 cut(s) 2583
Bsp68I TCGCGA 1 cut(s) 4054
BspANI GGCC 9 cut(s) 5, 895, 907, 1757, 2774, 3098, 3126, 3499, 3803
BspCNI CTCAG 7 cut(s) 71, 1291, 2152, 2596, 3244, 3481, 3808
BspDI ATCGAT 1 cut(s) 405
BspEI TCCGGA 1 cut(s) 3208
BspFNI CGCG 2 cut(s) 3132, 4054
BspHI TCATGA 1 cut(s) 3562
BspLI GGNNCC 4 cut(s) 22, 1369, 2616, 3018
BspMAI CTGCAG 1 cut(s) 2920
BspMI ACCTGC 2 cut(s) 2066, 2824
BspPI GGATC 7 cut(s) 542, 1760, 1846, 2574, 3133, 3219, 3904
BspQI GCTCTTC 2 cut(s) 1446, 3450
BspT104I TTCGAA 1 cut(s) 1067
BspTNI GGTCTC 2 cut(s) 284, 4043
BsrBI CCGCTC 2 cut(s) 329, 452
BsrDI GCAATG 1 cut(s) 3769
BsrI ACTGG 5 cut(s) 517, 1781, 2616, 2689, 4029
BssECI CCNNGG 5 cut(s) 1494, 1712, 1790, 3019, 3020
BssHII GCGCGC 1 cut(s) 3130
BssNI GRCGYC 1 cut(s) 3469
Bst2UI CCWGG 7 cut(s) 292, 447, 1713, 1791, 2064, 3021, 3501
BstACI GRCGYC 1 cut(s) 3469
BstAPI GCANNNNNTGC 1 cut(s) 3053
BstBAI YACGTR 1 cut(s) 1424
BstBI TTCGAA 1 cut(s) 1067
BstDSI CCRYGG 1 cut(s) 1494
BstEII GGTNACC 1 cut(s) 2066
BstENI CCTNNNNNAGG 1 cut(s) 1626
BstF5I GGATG 4 cut(s) 227, 2581, 3785, 4045
BstFNI CGCG 2 cut(s) 3132, 4054
BstH2I RGCGCY 2 cut(s) 2796, 3676
BstHHI GCGC 5 cut(s) 2730, 2795, 3132, 3134, 3675
BstMWI GCNNNNNNNGC 4 cut(s) 1540, 1916, 3053, 3545
BstNI CCWGG 7 cut(s) 292, 447, 1713, 1791, 2064, 3021, 3501
BstNSI RCATGY 7 cut(s) 1332, 1484, 2133, 2177, 2380, 2520, 3961
BstPI GGTNACC 1 cut(s) 2066
BstSCI CCNGG 7 cut(s) 290, 445, 1711, 1789, 2062, 3019, 3499
BstSFI CTRYAG 5 cut(s) 975, 2916, 3369, 3934, 4128
BstSLI GKGCMC 1 cut(s) 2029
BstUI CGCG 2 cut(s) 3132, 4054
BstV1I GCAGC 3 cut(s) 1807, 1972, 2902
BstV2I GAAGAC 4 cut(s) 1341, 3391, 3472, 3475
BstX2I RGATCY 7 cut(s) 1465, 1765, 1838, 2566, 3138, 3211, 3909
BstYI RGATCY 7 cut(s) 1465, 1765, 1838, 2566, 3138, 3211, 3909
Bsu15I ATCGAT 1 cut(s) 405
BsuI GTATCC 3 cut(s) 2121, 3018, 3650
BsuRI GGCC 9 cut(s) 5, 895, 907, 1757, 2774, 3098, 3126, 3499, 3803
BsuTUI ATCGAT 1 cut(s) 405
BtgI CCRYGG 1 cut(s) 1494
BtgZI GCGATG 1 cut(s) 4035
BtrI CACGTC 1 cut(s) 2956
BtsCI GGATG 4 cut(s) 227, 2581, 3785, 4045
BtsI GCAGTG 1 cut(s) 493
BtsIMutI CAGTG 6 cut(s) 493, 1300, 1446, 2027, 3285, 4030
BtuMI TCGCGA 1 cut(s) 4054
BveI ACCTGC 2 cut(s) 2066, 2824
CaiI CAGNNNCTG 2 cut(s) 1868, 2684
CciI TCATGA 1 cut(s) 3562
CfoI GCGC 5 cut(s) 2730, 2795, 3132, 3134, 3675
Cfr13I GGNCC 6 cut(s) 294, 1367, 1787, 1897, 2773, 3016
ClaI ATCGAT 1 cut(s) 405
CseI GACGC 3 cut(s) 3278, 3385, 3537
CsiI ACCWGGT 1 cut(s) 2062
Csp6I GTAC 7 cut(s) 455, 1049, 1701, 2682, 2691, 3159, 4122
CviQI GTAC 7 cut(s) 455, 1049, 1701, 2682, 2691, 3159, 4122
DraI TTTAAA 2 cut(s) 469, 1024
DraIII CACNNNGTG 1 cut(s) 1295
DrdI GACNNNNNNGTC 1 cut(s) 3468
DriI GACNNNNNGTC 1 cut(s) 2177
DseDI GACNNNNNNGTC 1 cut(s) 3468
EaeI YGGCCR 2 cut(s) 893, 3124
Eam1105I GACNNNNNGTC 1 cut(s) 2177
EciI GGCGGA 2 cut(s) 4, 200
Eco147I AGGCCT 1 cut(s) 3098
Eco31I GGTCTC 2 cut(s) 284, 4043
Eco32I GATATC 1 cut(s) 4135
Eco47I GGWCC 5 cut(s) 294, 1367, 1787, 1897, 3016
Eco47III AGCGCT 1 cut(s) 3674
Eco57I CTGAAG 8 cut(s) 435, 855, 1470, 1890, 1896, 2142, 3843, 4131
Eco88I CYCGRG 1 cut(s) 1732
Eco91I GGTNACC 1 cut(s) 2066
EcoNI CCTNNNNNAGG 1 cut(s) 1626
EcoO109I RGGNCCY 1 cut(s) 1367
EcoO65I GGTNACC 1 cut(s) 2066
EcoRI GAATTC 3 cut(s) 2842, 3827, 3890
EcoRII CCWGG 7 cut(s) 290, 445, 1711, 1789, 2062, 3019, 3499
EcoRV GATATC 1 cut(s) 4135
EcoT22I ATGCAT 1 cut(s) 1330
Esp3I CGTCTC 3 cut(s) 338, 3454, 3555
FalI AAGNNNNNCTT 6 cut(s) 1518, 1550, 1536, 1568, 3467, 3499
FaqI GGGAC 3 cut(s) 2298, 3002, 3200
FauI CCCGC 1 cut(s) 2428
FauNDI CATATG 1 cut(s) 2186
FblI GTMKAC 3 cut(s) 2217, 2534, 2593
Fnu4HI GCNGC 6 cut(s) 1796, 1986, 2451, 2916, 3249, 3926
FokI GGATG 4 cut(s) 214, 2588, 3792, 4032
Fsp4HI GCNGC 6 cut(s) 1796, 1986, 2451, 2916, 3249, 3926
FspBI CTAG 6 cut(s) 747, 1362, 2094, 2633, 2694, 3495
GlaI GCGC 5 cut(s) 2729, 2794, 3131, 3133, 3674
GluI GCNGC 6 cut(s) 1796, 1986, 2451, 2916, 3249, 3926
GsuI CTGGAG 2 cut(s) 534, 2388
HaeII RGCGCY 2 cut(s) 2796, 3676
HaeIII GGCC 9 cut(s) 5, 895, 907, 1757, 2774, 3098, 3126, 3499, 3803
HapII CCGG 4 cut(s) 18, 33, 276, 3209
HgaI GACGC 3 cut(s) 3278, 3385, 3537
HhaI GCGC 5 cut(s) 2730, 2795, 3132, 3134, 3675
Hin1I GRCGYC 1 cut(s) 3469
Hin6I GCGC 5 cut(s) 2728, 2793, 3130, 3132, 3673
HinP1I GCGC 5 cut(s) 2728, 2793, 3130, 3132, 3673
HincII GTYRAC 4 cut(s) 1957, 2182, 2594, 3003
HindII GTYRAC 4 cut(s) 1957, 2182, 2594, 3003
HindIII AAGCTT 1 cut(s) 2386
HpaI GTTAAC 1 cut(s) 1957
HpaII CCGG 4 cut(s) 18, 33, 276, 3209
HphI GGTGA 5 cut(s) 123, 827, 896, 2078, 2090
Hpy99I CGWCG 2 cut(s) 203, 239
HpyAV CCTTC 8 cut(s) 165, 359, 369, 679, 1572, 1747, 2976, 3614
HpyCH4IV ACGT 5 cut(s) 1423, 2955, 2999, 3197, 3469
HpyF10VI GCNNNNNNNGC 4 cut(s) 1540, 1916, 3053, 3545
HpySE526I ACGT 5 cut(s) 1423, 2955, 2999, 3197, 3469
Hsp92I GRCGYC 1 cut(s) 3469
HspAI GCGC 5 cut(s) 2728, 2793, 3130, 3132, 3673
Kpn2I TCCGGA 1 cut(s) 3208
KspAI GTTAAC 1 cut(s) 1957
LguI GCTCTTC 2 cut(s) 1446, 3450
LmnI GCTCC 6 cut(s) 20, 449, 2138, 2299, 3348, 3536
Lsp1109I GCAGC 3 cut(s) 1807, 1972, 2902
LweI GCATC 6 cut(s) 766, 1164, 1897, 3346, 3856, 4068
MabI ACCWGGT 1 cut(s) 2062
MaeI CTAG 6 cut(s) 747, 1362, 2094, 2633, 2694, 3495
MaeII ACGT 5 cut(s) 1423, 2955, 2999, 3197, 3469
MaeIII GTNAC 8 cut(s) 1287, 1444, 1508, 1600, 1930, 2066, 3147, 4093
MbiI CCGCTC 2 cut(s) 329, 452
MflI RGATCY 7 cut(s) 1465, 1765, 1838, 2566, 3138, 3211, 3909
MhlI GDGCHC 2 cut(s) 2029, 2268
MlsI TGGCCA 1 cut(s) 3126
MluNI TGGCCA 1 cut(s) 3126
MlyI GAGTC 3 cut(s) 637, 647, 2082
MmeI TCCRAC 5 cut(s) 75, 1111, 1636, 2255, 3246
Mox20I TGGCCA 1 cut(s) 3126
Mph1103I ATGCAT 1 cut(s) 1330
MroI TCCGGA 1 cut(s) 3208
MroXI GAANNNNTTC 6 cut(s) 348, 1184, 2412, 2846, 3603, 3890
MscI TGGCCA 1 cut(s) 3126
MslI CAYNNNNRTG 5 cut(s) 148, 1905, 2076, 2189, 3881
Msp20I TGGCCA 1 cut(s) 3126
MspA1I CMGCKG 4 cut(s) 2423, 2453, 3251, 3436
MspI CCGG 4 cut(s) 18, 33, 276, 3209
MspR9I CCNGG 7 cut(s) 292, 447, 1713, 1791, 2064, 3021, 3501
Mva1269I GAATGC 2 cut(s) 1063, 1880
MvaI CCWGG 7 cut(s) 292, 447, 1713, 1791, 2064, 3021, 3501
MvnI CGCG 2 cut(s) 3132, 4054
MwoI GCNNNNNNNGC 4 cut(s) 1540, 1916, 3053, 3545
NdeI CATATG 1 cut(s) 2186
NlaIV GGNNCC 4 cut(s) 22, 1369, 2616, 3018
NmuCI GTSAC 5 cut(s) 1287, 1444, 1930, 2066, 4093
NruI TCGCGA 1 cut(s) 4054
NsiI ATGCAT 1 cut(s) 1330
NspI RCATGY 7 cut(s) 1332, 1484, 2133, 2177, 2380, 2520, 3961
NspV TTCGAA 1 cut(s) 1067
PaeI GCATGC 2 cut(s) 1484, 2133
PagI TCATGA 1 cut(s) 3562
PaqCI CACCTGC 1 cut(s) 2066
PasI CCCWGGG 1 cut(s) 3020
PauI GCGCGC 1 cut(s) 3130
PceI AGGCCT 1 cut(s) 3098
PciI ACATGT 2 cut(s) 2376, 3957
PciSI GCTCTTC 2 cut(s) 1446, 3450
PcsI WCGNNNNNNNCGW 1 cut(s) 1602
PctI GAATGC 2 cut(s) 1063, 1880
PdmI GAANNNNTTC 6 cut(s) 348, 1184, 2412, 2846, 3603, 3890
PfoI TCCNGGA 1 cut(s) 445
PkrI GCNGC 6 cut(s) 1797, 1987, 2452, 2917, 3250, 3927
PleI GAGTC 3 cut(s) 636, 646, 2082
PpsI GAGTC 3 cut(s) 636, 646, 2082
Ppu21I YACGTR 1 cut(s) 1424
PpuMI RGGWCCY 1 cut(s) 1367
PscI ACATGT 2 cut(s) 2376, 3957
PsiI TTATAA 1 cut(s) 1094
Psp5II RGGWCCY 1 cut(s) 1367
Psp6I CCWGG 7 cut(s) 290, 445, 1711, 1789, 2062, 3019, 3499
PspEI GGTNACC 1 cut(s) 2066
PspGI CCWGG 7 cut(s) 290, 445, 1711, 1789, 2062, 3019, 3499
PspN4I GGNNCC 4 cut(s) 22, 1369, 2616, 3018
PspPI GGNCC 6 cut(s) 294, 1367, 1787, 1897, 2773, 3016
PspPPI RGGWCCY 1 cut(s) 1367
PstI CTGCAG 1 cut(s) 2920
PstNI CAGNNNCTG 2 cut(s) 1868, 2684
PsuI RGATCY 7 cut(s) 1465, 1765, 1838, 2566, 3138, 3211, 3909
PteI GCGCGC 1 cut(s) 3130
PvuII CAGCTG 1 cut(s) 3436
RruI TCGCGA 1 cut(s) 4054
RsaI GTAC 7 cut(s) 456, 1050, 1702, 2683, 2692, 3160, 4123
RsaNI GTAC 7 cut(s) 455, 1049, 1701, 2682, 2691, 3159, 4122
RseI CAYNNNNRTG 5 cut(s) 148, 1905, 2076, 2189, 3881
SalI GTCGAC 1 cut(s) 2592
SapI GCTCTTC 2 cut(s) 1446, 3450
SatI GCNGC 6 cut(s) 1796, 1986, 2451, 2916, 3249, 3926
Sau96I GGNCC 6 cut(s) 294, 1367, 1787, 1897, 2773, 3016
ScaI AGTACT 1 cut(s) 2692
SchI GAGTC 3 cut(s) 637, 647, 2082
ScrFI CCNGG 7 cut(s) 292, 447, 1713, 1791, 2064, 3021, 3501
SduI GDGCHC 2 cut(s) 2029, 2268
SexAI ACCWGGT 1 cut(s) 2062
SfaNI GCATC 6 cut(s) 766, 1164, 1897, 3346, 3856, 4068
SfcI CTRYAG 5 cut(s) 975, 2916, 3369, 3934, 4128
SfuI TTCGAA 1 cut(s) 1067
SinI GGWCC 5 cut(s) 294, 1367, 1787, 1897, 3016
SmiMI CAYNNNNRTG 5 cut(s) 148, 1905, 2076, 2189, 3881
SmlI CTYRAG 3 cut(s) 2900, 3509, 3689
SmoI CTYRAG 3 cut(s) 2900, 3509, 3689
SphI GCATGC 2 cut(s) 1484, 2133
SseBI AGGCCT 1 cut(s) 3098
SspI AATATT 1 cut(s) 2467
SspMI CTAG 6 cut(s) 747, 1362, 2094, 2633, 2694, 3495
StuI AGGCCT 1 cut(s) 3098
StyD4I CCNGG 7 cut(s) 290, 445, 1711, 1789, 2062, 3019, 3499
TaiI ACGT 5 cut(s) 1426, 2958, 3002, 3200, 3472
TaqI TCGA 9 cut(s) 201, 405, 591, 774, 791, 1031, 1067, 2593, 3879
TaqII GACCGA 1 cut(s) 2520
TatI WGTACW 1 cut(s) 2690
TauI GCSGC 3 cut(s) 2453, 3251, 3928
TscAI CASTG 6 cut(s) 493, 1300, 1453, 2034, 3292, 4030
TseFI GTSAC 5 cut(s) 1287, 1444, 1930, 2066, 4093
TseI GCWGC 3 cut(s) 1795, 1985, 2915
Tsp45I GTSAC 5 cut(s) 1287, 1444, 1930, 2066, 4093
TspGWI ACGGA 3 cut(s) 223, 540, 3443
TspRI CASTG 6 cut(s) 493, 1300, 1453, 2034, 3292, 4030
VpaK11BI GGWCC 5 cut(s) 294, 1367, 1787, 1897, 3016
XagI CCTNNNNNAGG 1 cut(s) 1626
XapI RAATTY 8 cut(s) 910, 1687, 2164, 2753, 2842, 3071, 3827, 3890
XbaI TCTAGA 1 cut(s) 1361
XceI RCATGY 7 cut(s) 1332, 1484, 2133, 2177, 2380, 2520, 3961
XmiI GTMKAC 3 cut(s) 2217, 2534, 2593
XmnI GAANNNNTTC 6 cut(s) 348, 1184, 2412, 2846, 3603, 3890
XspI CTAG 6 cut(s) 747, 1362, 2094, 2633, 2694, 3495
ZraI GACGTC 1 cut(s) 3470
ZrmI AGTACT 1 cut(s) 2692
Zsp2I ATGCAT 1 cut(s) 1330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.