RchiOBHm_Chr3g0493691

DNA ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0015031
Physical Location & Seq
Reverse (-)
40979844 .. 40991212
11369 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45643

Sequence Viewer

Length: 4209 bp
ATGTTCTTGATGACGGCTCTAGATTCTACGGACCTCTTCCTGACTGCCCTAACCGCCTTTTCCCACCCACCCAGACCCATACCCCGTCCCCTTGAACTCTCTCTCCCTCCAATTCCCTCCACATTCCCCCACTCCAAACTCATCCCCAGAACCCGTTTCGCCCTCGACGCATTCCGCTATGCCGGCGATTATTCCGTCTCTTACTTCCTCTCTCACTTCCACTCCGATCACTACGGCGGTCTCTCCCCCAATTGGTCCCAAGGCCTCGTCTTTTGCTCCCCCACCACCGCTCGTCTTCTCACAGACGTCCTCAACGTCTCTTCGCTTTTCGTGGTTCCTCTGCCGCTCGGACAGCCCCTTCTCATCGATGGATGCGAGGTCGTGCTTATTGACGCCAATCATTGCCCGGGCGCCGTTCAGTTCTTGTTCAAAGTTCCCGCCTCCAATGGAGACTTCCAGAGGTATGTTCACACTGGTGATTTTCGGTTTTCTGCTCCGATGAAGCTAGACCCTTTTCTCCGTAACTTTGTGGGTTCAGACGCCGTTTTCTTGGATACAACTTATTGCAATCCCAAATTCGTGTTTCCTTCACAAGAAGAATCTGTTGATTATATTTCTAGCTTGATAGAAAGAGTCGGAGGTGAACACGAAGTTTCTATGAAAAGTGTTCTGTTCCTTGTTGCTACATATGTGATTGGGAAAGAGAAGATTTTGATAGAAATTGCTCGTAGATGTAAGCGTAAAGTGTATGTGGATGCTCGAAAAATGGCGGTGCTGCGTGTTTTGGGTTACGGAGACAGTGGGGTGTTTACAGAGGATGAATGTGAGAGTGATGTGCATGTTGTTGGGTGGAATGTGTTGGGTGAGACTTGGCCATATTTCAGGCCAAATTTTGTGAAAATGGAGGAGATTATGGTTGAGAGGGGGTATTCCAGGGTTGTAGGGTTTGTCCCTACTGGCTGGACTTATGAAGTCAAGCGTAACAAGTTCTCGGTGAGGTCAAAGGATGCTTTTGAGATCCATCTTGTACCCTACAGTGAGCATTCAAACTATGATGAACTTAGAGAATACGTCAGGTTTTTGAAACCAAAGCGTGTGATTCCTACAGTGGGCTCCGATGTGGAAAAGATAGACAGTAAACAGGCCAGTAAAATGCAAAAGCATTTTGCTGGGTTAATTGATGAAATGGCCAACAAAAAAGAGTTCCTGCAGGGTTTTCATCGTGGGACTACAGAAGGAGGTGGGAAGGTTGACAGTGATGCCAAAGATTACACCATGGAGGGGCAGGATCAAGAGAAAAAGGCAACACCTTCAGATATTGATACTATGGGAGATACTAATGTAGGCTTTGGTAAGGAATTGTTGTCTCCTCTCCAAGAACCTGATTCACAAACTCCAATATTGTTAACTGATGAAAAAGCAGAGGAAATTATTCAACAACTTCGTGATTGTTTGCCGTCATGGGTCACTCGCCAGCAGATGCTAGATCTGATTGGGAGCTCAGGAGGGGACATTGTTGAAGCAGTCACTAAATTTTACGATCGTGAAACAGAATTTCATGATCAAGGAATTGCCTCTACAAGTGCCGTATCTGTATCCGAGACCAGCTCACTCTGTGACACAGCATCACCGACAAAAGCAGGTTCTGTTCATGCAAATATTGATGTTTCTTCAAGCCAAGCTCATATTTCACCCAACCCAAGAAATATTGTTAAAAGTGGCATTTCCCCTGGAAAAAGAGGGAGGAAGATCAGTAATAAAGTTAACAAGAAAGTAAAGTTACAGTCAAAATTGAATTTGTGTGGGCCAAAACAATCGACAATAACAAGGTTTTTTAGTAAAGTTTTGCCTGACGTTACAGAAAGTGCAGAGATTGGATCAGTAGGTGAACAAAACCCTAAAGATAAAAAATTACCAGATCATGTCACCCAATCATACAAGGATGCAATAGATCAATTTCTTCAGATAATAGATGGCAATGAATCATTAAAAAGCTATGCTGGTACCATTCTTCAAAAGGCAAATGGGGATATTAGTATGGCTGTGGATATACATTACCGTAATAATGAAGGCAGGTCTGATGAGAATGAAGTGGAGCTGGTGGTTGATGCAAAATCAGTTCAGTCCAAATGTGGTATAGATAACTCTTCTTCTGTCCAAAAAAAAATTGAATTAGGGAAAATAGGAGTGGTAGCTGATTTGTCTGTACGGGGGTCATTACCAGATAACGTCGCTGCAACTTCTGTGTCACTACCACCAGAAAAATACAATCCTGTAGATCATGCATGCTGGAGCAATGGACAACATGCTCCATATTTACATCTTGCACGAACCTTTGACTTACTAGAGGATGAAAAAGGCAAGATAAAGGCTACTTCTATGCTCTGCAATATGTTCAGAAGTTTGCTTGCCCTGTCTCCTGATGATGTGCTGCCTTCAGTTTATTTGTGCACCAATAAGATAGCTGCAGACCACGAAAATGTGGAACTGAACATTGGTGGAAGTTTGGTCACAGCAGCACTGGAGGACGCATGTGGGACAAGCAGATCCAAAATCAGGGATATGTACAATGAATTGGGAGATCTTGGTGATGTTGCTCAAGCATGCCGGCAGACACAGACTTTGCTTGCCCCTCCTCCACCACTTCTAATAAAAGATGTATTTTCTGCACTCCGGAAGATAAGTGTACAAACAGGTACTGGAAGCAGTGCTCGAAAGAGAAGCCTGATTGTGAATCTCTTGCGTTCTTGCAGAGAGAAAGAGATGAAGTTCCTTGTTAGAACTTTGGTTCGAAACTTACGTATCGGAGCAATGATGAAAACTGTCCTACCTGCTCTGGCTCAAGCTGTTGTTCTGAATTCCTTCCATAGCTGTAATCACGAAGGAACTATAGAAAGTTTGAAGGACAAGCTTCAGCGCCATTCAGCAGCTGTAGTAGAAGCATACAATGTACTGCCCAATTTGGATTTGGTCATTCCTTCTCTCATGAAAAGAGGCATTGGATTCTCCTCATCAACTTTGTCAATGGTTCCAGGAATACCTATCAAGCCAATGCTTGCAAGAATCACAAATGGTGTTCAGCAAGCACTGAAGCTATTTGAAAATAAAGCTTTTACATGTGAATACAAATATGATGGCCAGCGTGCACAAATTCATAAACTAGTTGATGGGTCCATGCATGTTTTTACAAGAAATGGGGATGAGTCAACTTCGAGATTTCCAGATCTGATTAATATAATCAATCAATCTTGTAAACCCGATGCAGTGACTTTCATACTGGATGCAGAGGTTGTTGGAGTTGATAGGAAGAATGGTTGCAAGCTAATGTCCTTCCAAGAATTATCTTCACGTGGGAGAGGGAGCAGAGATGCATCAATAACGTTGGATAGCATAAAGGTCAACATTTGCGTCTTTGTCTTCGACATCATGTTTGCCAATGGACAACAGTTGTTAAGCTTTCCTCTGCGGAGAAGGCGAAAGTATTTGAAAGAGTTGTTCTACGATGAGAAGTTGGGTTACTTTGAATATGCCAAGGAAATGACAGTTGAGGCAGAGGATGCTTGTTTAACCAGTGAAGCGACATTAACCAAAATAAACTCTTTCCTTGAAAATGCATTTGTTTCCTCTTGTGAAGGGATTATAGTTAAATCTTTAGATGTTGATGCTGGATATTCTCCATCAAAGCGTACAGATACTTGGTTAAAGGTCAAGCGAGACTACATGGAGGGATTGGGCGATACTCTTGATTTAGTCCCAATTGGAGCTTGGCATGGAAACGGGAGAAAGGCAGGCTGGTACAGTCCATTTCTCATGGCATGTTACAATCCTGAAACTGAAGAATTTCAAAGCGTCTGTCGTGTTATGTCTGGGTTTTCAGATTTATTTTATACAGAGATGAAAAGTTTTTATTCTGGGGACAAGATCTTGGCCAAAAAACCTCCTTATTATAGAACAGCTGAGGTGCCTGATTTGTGGTTTTCTCCTGAGCTTGTATGGGAAATAAGAGGTGCAGACTTTACAGTGTCTCCAGTTCACCAGGCTGCTTTAGGTCTAGTTCATCCATCACGTGGCATTTCCATTCGATTCCCAAGATACATTCGCACTGTGACTGATAGAAAACCAGATGAATGTAGCACAGCTTCTGATTTAGCTGAAATGTTTCATTCTCAGACGAGGAAGATGGATGTGGCTTCTGAAGATGGCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000012 GO:0000726 GO:0002237 GO:0003006 GO:0003674 GO:0003824 GO:0003909 GO:0003910 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006266 GO:0006271 GO:0006273 GO:0006281 GO:0006302 GO:0006303 GO:0006304 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009314 GO:0009409 GO:0009411 GO:0009416 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009791 GO:0009845 GO:0009987 GO:0010033 GO:0010154 GO:0010225 GO:0010243 GO:0015074 GO:0016070 GO:0016874 GO:0016886 GO:0022414 GO:0022616 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0033554 GO:0034641 GO:0034645 GO:0035510 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044728 GO:0046483 GO:0046677 GO:0048316 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051103 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0065007 GO:0070013 GO:0070988 GO:0071704 GO:0080111 GO:0090304 GO:0090351 GO:0140097 GO:1901360 GO:1901576 GO:1901652 GO:1901698 GO:1901700 GO:1904975 GO:2000683 GO:2000685
KEGG Pathways
Metabolic & Signaling
ko03030 ko03410 ko03420 ko03430 map03030 map03410 map03420 map03430
Pfam Domains
Protein Families
DNA_ligase_A_C DNA_ligase_A_M DNA_ligase_A_N DRMBL

Protein Analysis

1402

Amino Acids

155.93

Weight (kDa)

6.38

Isoelectric Point (pI)

45.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DRMBL PF07522 268 - 371 2.4e-20 DNA repair metallo-beta-lactamase
DNA_ligase_A_N PF04675 771 - 947 6.8e-43 DNA ligase N terminus
DNA_ligase_A_M PF01068 1017 - 1237 1.3e-46 ATP dependent DNA ligase domain
DNA_ligase_A_C PF04679 1263 - 1370 3.2e-16 ATP dependent DNA ligase C terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015031)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66730
fragaria_vesca FvH4_1g29000 FvH4_1g29000 FvH4_1g29000
malus_domestica MD00G1054100.v1.1
prunus_persica Prupe.3G150300_v2.0.a1
pyrus_communis pycom17g25560
rosa_chinensis RchiOBHm_Chr3g0493691 RchiOBHm_Chr3g0493951
rosa_laevigata RLG00000022694
rosa_roxburghii Rroxscaffold_6G00391370
rosa_rugosa Rorug03G0263400 Rorug03G0263500 Rorug03G0263600
rosa_samantha Rh3AG312500 Rh3AG313200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 309
Acc36I ACCTGC 3 cut(s) 1631, 2064, 2839
Acc65I GGTACC 1 cut(s) 2003
AccB1I GGYRCC 3 cut(s) 410, 2003, 3961
AccB7I CCANNNNNTGG 1 cut(s) 4067
AccBSI CCGCTC 2 cut(s) 290, 346
AccIII TCCGGA 1 cut(s) 2673
AciI CCGC 8 cut(s) 54, 175, 237, 288, 344, 438, 770, 3466
AclI AACGTT 1 cut(s) 3380
AclWI GGATC 4 cut(s) 1012, 1296, 1885, 2541
AcoI YGGCCR 4 cut(s) 872, 1188, 3136, 3927
AcsI RAATTY 8 cut(s) 575, 889, 1532, 1553, 1795, 2857, 3150, 3839
AcuI CTGAAG 6 cut(s) 1296, 1946, 2421, 2897, 3110, 3855
AcvI CACGTG 2 cut(s) 3350, 4067
AcyI GRCGYC 4 cut(s) 306, 393, 411, 540
AdeI CACNNNGTG 1 cut(s) 1616
AfaI GTAC 9 cut(s) 1029, 2005, 2208, 2567, 2688, 2698, 2952, 3688, 3797
AfiI CCNNNNNNNGG 5 cut(s) 252, 1109, 1281, 2556, 4067
AflIII ACRYGT 1 cut(s) 3116
AhlI ACTAGT 1 cut(s) 3160
AjnI CCWGG 4 cut(s) 932, 1729, 3031, 4035
AjuI GAANNNNNNNTTGG 4 cut(s) 437, 469, 2478, 2510
AleI CACNNNNGTG 1 cut(s) 474
AloI GAACNNNNNNTCC 2 cut(s) 87, 119
Alw21I GWGCWC 4 cut(s) 1502, 2453, 2713, 3148
Alw44I GTGCAC 2 cut(s) 2449, 3144
AlwI GGATC 4 cut(s) 1012, 1296, 1885, 2541
AlwNI CAGNNNCTG 4 cut(s) 1646, 2699, 2930, 4142
Ama87I CYCGRG 1 cut(s) 406
Aor13HI TCCGGA 1 cut(s) 2673
AoxI GGCC 8 cut(s) 262, 872, 884, 1143, 1188, 1805, 3136, 3927
ApaLI GTGCAC 2 cut(s) 2449, 3144
ApeKI GCWGC 8 cut(s) 775, 2234, 2431, 2465, 2516, 2927, 4040, 4203
ApoI RAATTY 8 cut(s) 575, 889, 1532, 1553, 1795, 2857, 3150, 3839
ArsI GACNNNNNNTTYG 6 cut(s) 2605, 2637, 3413, 3445, 3838, 3870
AseI ATTAAT 1 cut(s) 3231
Asp700I GAANNNNTTC 4 cut(s) 1431, 2861, 3840, 4158
Asp718I GGTACC 1 cut(s) 2003
AspLEI GCGC 2 cut(s) 413, 2919
AspS9I GGNCC 4 cut(s) 31, 255, 1805, 3171
AsuC2I CCSGG 2 cut(s) 407, 408
AsuII TTCGAA 1 cut(s) 2791
AvaI CYCGRG 1 cut(s) 406
AvaII GGWCC 3 cut(s) 31, 255, 3171
BaeGI GKGCMC 2 cut(s) 2453, 3148
BaeI ACNNNNGTAYC 4 cut(s) 1326, 1359, 2785, 2818
BalI TGGCCA 4 cut(s) 874, 1190, 3138, 3929
BanI GGYRCC 3 cut(s) 410, 2003, 3961
BanII GRGCYC 2 cut(s) 1115, 1502
BarI GAAGNNNNNNTAC 4 cut(s) 3500, 3532, 4123, 4155
BbrPI CACGTG 2 cut(s) 3350, 4067
BbsI GAAGAC 2 cut(s) 287, 3409
Bbv12I GWGCWC 4 cut(s) 1502, 2453, 2713, 3148
BbvCI CCTCAGC 1 cut(s) 3957
BbvI GCAGC 7 cut(s) 762, 2221, 2418, 2452, 2528, 2939, 4027
BccI CCATC 9 cut(s) 362, 1029, 1967, 3128, 3161, 3685, 4069, 4174, 4193
BceAI ACGGC 6 cut(s) 30, 250, 398, 527, 1441, 1571
BciT130I CCWGG 4 cut(s) 934, 1731, 3033, 4037
BciVI GTATCC 2 cut(s) 547, 1606
BclI TGATCA 1 cut(s) 1561
BcnI CCSGG 2 cut(s) 407, 408
BcuI ACTAGT 1 cut(s) 3160
BfaI CTAG 7 cut(s) 20, 506, 618, 1484, 2345, 3161, 4052
BfmI CTRYAG 8 cut(s) 1033, 1104, 1208, 1230, 2274, 2466, 2889, 2931
BfoI RGCGCY 2 cut(s) 414, 2920
BfuAI ACCTGC 3 cut(s) 1631, 2064, 2839
BfuI GTATCC 2 cut(s) 547, 1606
BglII AGATCT 4 cut(s) 1486, 2581, 3223, 3921
BisI GCNGC 9 cut(s) 344, 776, 2235, 2432, 2466, 2517, 2928, 4041, 4204
BlsI GCNGC 9 cut(s) 345, 777, 2236, 2433, 2467, 2518, 2929, 4042, 4205
Bme1390I CCNGG 6 cut(s) 407, 408, 934, 1731, 3033, 4037
Bme18I GGWCC 3 cut(s) 31, 255, 3171
BmeT110I CYCGRG 1 cut(s) 406
BmgT120I GGNCC 4 cut(s) 31, 255, 1805, 3171
BmiI GGNNCC 8 cut(s) 257, 336, 412, 1114, 2005, 3030, 3172, 3963
BmrFI CCNGG 6 cut(s) 407, 408, 934, 1731, 3033, 4037
BpiI GAAGAC 2 cut(s) 287, 3409
BplI GAGNNNNNCTC 2 cut(s) 1592, 1624
BpmI CTGGAG 3 cut(s) 2311, 2543, 4011
Bpu10I CCTNAGC 3 cut(s) 1501, 3957, 3984
Bpu14I TTCGAA 1 cut(s) 2791
BpuEI CTTGAG 2 cut(s) 2583, 2826
BpuMI CCSGG 2 cut(s) 407, 408
Bsa29I ATCGAT 1 cut(s) 366
BsaAI YACGTR 3 cut(s) 2801, 3350, 4067
BsaHI GRCGYC 4 cut(s) 306, 393, 411, 540
BsaI GGTCTC 2 cut(s) 245, 1595
BsaJI CCNNGG 6 cut(s) 259, 406, 933, 1275, 1729, 3531
BsaWI WCCGGW 1 cut(s) 2673
BsaXI ACNNNNNCTCC 6 cut(s) 87, 117, 206, 236, 4009, 4039
Bsc4I CCNNNNNNNGG 5 cut(s) 252, 1109, 1281, 2556, 4067
Bse118I RCCGGY 2 cut(s) 182, 2607
Bse1I ACTGG 8 cut(s) 478, 961, 1146, 2526, 2704, 3282, 3570, 4028
Bse3DI GCAATG 4 cut(s) 400, 1984, 2302, 2817
BseAI TCCGGA 1 cut(s) 2673
BseBI CCWGG 4 cut(s) 934, 1731, 3033, 4037
BseCI ATCGAT 1 cut(s) 366
BseDI CCNNGG 6 cut(s) 259, 406, 933, 1275, 1729, 3531
BseLI CCNNNNNNNGG 5 cut(s) 252, 1109, 1281, 2556, 4067
BseMI GCAATG 4 cut(s) 400, 1984, 2302, 2817
BseMII CTCAG 4 cut(s) 1515, 3948, 3975, 4181
BseNI ACTGG 8 cut(s) 478, 961, 1146, 2526, 2704, 3282, 3570, 4028
BseRI GAGGAG 4 cut(s) 920, 1359, 2625, 2998
BseSI GKGCMC 2 cut(s) 2453, 3148
BseXI GCAGC 7 cut(s) 762, 2221, 2418, 2452, 2528, 2939, 4027
BseYI CCCAGC 1 cut(s) 1169
BsgI GTGCAG 3 cut(s) 1887, 2652, 4029
Bsh1285I CGRYCG 1 cut(s) 1543
BshFI GGCC 8 cut(s) 264, 874, 886, 1145, 1190, 1807, 3138, 3929
BshNI GGYRCC 3 cut(s) 410, 2003, 3961
BshVI ATCGAT 1 cut(s) 366
BsiEI CGRYCG 1 cut(s) 1543
BsiHKAI GWGCWC 4 cut(s) 1502, 2453, 2713, 3148
BsiHKCI CYCGRG 1 cut(s) 406
BsiSI CCGG 4 cut(s) 183, 407, 2608, 2674
BslFI GGGAC 8 cut(s) 72, 241, 935, 1240, 1523, 2551, 3737, 3929
BslI CCNNNNNNNGG 5 cut(s) 252, 1109, 1281, 2556, 4067
BsmBI CGTCTC 2 cut(s) 202, 322
BsmFI GGGAC 8 cut(s) 72, 241, 935, 1240, 1523, 2551, 3737, 3929
BsmI GAATGC 2 cut(s) 170, 1042
BsnI GGCC 8 cut(s) 264, 874, 886, 1145, 1190, 1807, 3138, 3929
Bso31I GGTCTC 2 cut(s) 245, 1595
BsoBI CYCGRG 1 cut(s) 406
Bsp119I TTCGAA 1 cut(s) 2791
Bsp1286I GDGCHC 5 cut(s) 1115, 1502, 2453, 2713, 3148
Bsp13I TCCGGA 1 cut(s) 2673
Bsp1407I TGTACA 2 cut(s) 2565, 2686
Bsp19I CCATGG 1 cut(s) 1275
BspACI CCGC 8 cut(s) 54, 175, 237, 288, 344, 438, 770, 3466
BspANI GGCC 8 cut(s) 264, 874, 886, 1145, 1190, 1807, 3138, 3929
BspCNI CTCAG 4 cut(s) 1514, 3949, 3976, 4180
BspDI ATCGAT 1 cut(s) 366
BspEI TCCGGA 1 cut(s) 2673
BspHI TCATGA 2 cut(s) 1558, 2985
BspLI GGNNCC 8 cut(s) 257, 336, 412, 1114, 2005, 3030, 3172, 3963
BspMAI CTGCAG 2 cut(s) 1212, 2470
BspMI ACCTGC 3 cut(s) 1631, 2064, 2839
BspPI GGATC 4 cut(s) 1012, 1296, 1885, 2541
BspT104I TTCGAA 1 cut(s) 2791
BspT107I GGYRCC 3 cut(s) 410, 2003, 3961
BspTNI GGTCTC 2 cut(s) 245, 1595
BsrBI CCGCTC 2 cut(s) 290, 346
BsrDI GCAATG 4 cut(s) 400, 1984, 2302, 2817
BsrFI RCCGGY 2 cut(s) 182, 2607
BsrGI TGTACA 2 cut(s) 2565, 2686
BsrI ACTGG 8 cut(s) 478, 961, 1146, 2526, 2704, 3282, 3570, 4028
BssAI RCCGGY 2 cut(s) 182, 2607
BssECI CCNNGG 6 cut(s) 259, 406, 933, 1275, 1729, 3531
BssNI GRCGYC 4 cut(s) 306, 393, 411, 540
BssT1I CCWWGG 3 cut(s) 259, 1275, 3531
Bst2UI CCWGG 4 cut(s) 934, 1731, 3033, 4037
Bst6I CTCTTC 3 cut(s) 41, 325, 2152
BstACI GRCGYC 4 cut(s) 306, 393, 411, 540
BstAPI GCANNNNNTGC 1 cut(s) 3557
BstAUI TGTACA 2 cut(s) 2565, 2686
BstBAI YACGTR 3 cut(s) 2801, 3350, 4067
BstBI TTCGAA 1 cut(s) 2791
BstDEI CTNAG 5 cut(s) 1061, 1501, 3957, 3984, 4167
BstDSI CCRYGG 1 cut(s) 1275
BstH2I RGCGCY 2 cut(s) 414, 2920
BstHHI GCGC 2 cut(s) 413, 2919
BstMCI CGRYCG 1 cut(s) 1543
BstMWI GCNNNNNNNGC 6 cut(s) 53, 167, 183, 352, 3472, 3557
BstNI CCWGG 4 cut(s) 934, 1731, 3033, 4037
BstNSI RCATGY 8 cut(s) 842, 2289, 2309, 2535, 2607, 3120, 3182, 3819
BstSCI CCNGG 6 cut(s) 405, 406, 932, 1729, 3031, 4035
BstSFI CTRYAG 8 cut(s) 1033, 1104, 1208, 1230, 2274, 2466, 2889, 2931
BstSLI GKGCMC 2 cut(s) 2453, 3148
BstSNI TACGTA 1 cut(s) 2801
BstV1I GCAGC 7 cut(s) 762, 2221, 2418, 2452, 2528, 2939, 4027
BstV2I GAAGAC 2 cut(s) 287, 3409
BstX2I RGATCY 6 cut(s) 1017, 1486, 2546, 2581, 3223, 3921
BstYI RGATCY 6 cut(s) 1017, 1486, 2546, 2581, 3223, 3921
Bsu15I ATCGAT 1 cut(s) 366
BsuI GTATCC 2 cut(s) 547, 1606
BsuRI GGCC 8 cut(s) 264, 874, 886, 1145, 1190, 1807, 3138, 3929
BsuTUI ATCGAT 1 cut(s) 366
BtgI CCRYGG 1 cut(s) 1275
BtsI GCAGTG 2 cut(s) 2713, 3270
BveI ACCTGC 3 cut(s) 1631, 2064, 2839
CaiI CAGNNNCTG 4 cut(s) 1646, 2699, 2930, 4142
CciI TCATGA 2 cut(s) 1558, 2985
CfoI GCGC 2 cut(s) 413, 2919
Cfr10I RCCGGY 2 cut(s) 182, 2607
Cfr13I GGNCC 4 cut(s) 31, 255, 1805, 3171
Cfr9I CCCGGG 1 cut(s) 406
ClaI ATCGAT 1 cut(s) 366
CseI GACGC 6 cut(s) 176, 401, 548, 2537, 3397, 3838
Csp6I GTAC 9 cut(s) 1028, 2004, 2207, 2566, 2687, 2697, 2951, 3687, 3796
CviQI GTAC 9 cut(s) 1028, 2004, 2207, 2566, 2687, 2697, 2951, 3687, 3796
DdeI CTNAG 5 cut(s) 1061, 1501, 3957, 3984, 4167
DinI GGCGCC 1 cut(s) 412
DraIII CACNNNGTG 1 cut(s) 1616
EaeI YGGCCR 4 cut(s) 872, 1188, 3136, 3927
Eam1104I CTCTTC 3 cut(s) 41, 325, 2152
EarI CTCTTC 3 cut(s) 41, 325, 2152
Ecl136II GAGCTC 1 cut(s) 1500
Eco105I TACGTA 1 cut(s) 2801
Eco130I CCWWGG 3 cut(s) 259, 1275, 3531
Eco147I AGGCCT 1 cut(s) 264
Eco24I GRGCYC 2 cut(s) 1115, 1502
Eco31I GGTCTC 2 cut(s) 245, 1595
Eco47I GGWCC 3 cut(s) 31, 255, 3171
Eco53kI GAGCTC 1 cut(s) 1500
Eco57I CTGAAG 6 cut(s) 1296, 1946, 2421, 2897, 3110, 3855
Eco72I CACGTG 2 cut(s) 3350, 4067
Eco88I CYCGRG 1 cut(s) 406
EcoICRI GAGCTC 1 cut(s) 1500
EcoRI GAATTC 1 cut(s) 2857
EcoRII CCWGG 4 cut(s) 932, 1729, 3031, 4035
EcoT14I CCWWGG 3 cut(s) 259, 1275, 3531
EcoT22I ATGCAT 4 cut(s) 2287, 3180, 3373, 3616
EcoT38I GRGCYC 2 cut(s) 1115, 1502
EgeI GGCGCC 1 cut(s) 412
EheI GGCGCC 1 cut(s) 412
ErhI CCWWGG 3 cut(s) 259, 1275, 3531
Esp3I CGTCTC 2 cut(s) 202, 322
FaqI GGGAC 8 cut(s) 72, 241, 935, 1240, 1523, 2551, 3737, 3929
FauI CCCGC 1 cut(s) 445
FauNDI CATATG 1 cut(s) 688
FbaI TGATCA 1 cut(s) 1561
Fnu4HI GCNGC 9 cut(s) 344, 776, 2235, 2432, 2466, 2517, 2928, 4041, 4204
FriOI GRGCYC 2 cut(s) 1115, 1502
Fsp4HI GCNGC 9 cut(s) 344, 776, 2235, 2432, 2466, 2517, 2928, 4041, 4204
FspBI CTAG 7 cut(s) 20, 506, 618, 1484, 2345, 3161, 4052
GlaI GCGC 2 cut(s) 412, 2918
GluI GCNGC 9 cut(s) 344, 776, 2235, 2432, 2466, 2517, 2928, 4041, 4204
GsaI CCCAGC 1 cut(s) 1173
GsuI CTGGAG 3 cut(s) 2311, 2543, 4011
HaeII RGCGCY 2 cut(s) 414, 2920
HaeIII GGCC 8 cut(s) 264, 874, 886, 1145, 1190, 1807, 3138, 3929
HapII CCGG 4 cut(s) 183, 407, 2608, 2674
HgaI GACGC 6 cut(s) 176, 401, 548, 2537, 3397, 3838
HhaI GCGC 2 cut(s) 413, 2919
Hin1I GRCGYC 4 cut(s) 306, 393, 411, 540
Hin6I GCGC 2 cut(s) 411, 2917
HinP1I GCGC 2 cut(s) 411, 2917
HincII GTYRAC 5 cut(s) 1252, 1407, 1765, 3207, 3400
HindII GTYRAC 5 cut(s) 1252, 1407, 1765, 3207, 3400
HindIII AAGCTT 3 cut(s) 2909, 3108, 3454
HpaI GTTAAC 2 cut(s) 1407, 1765
HpaII CCGG 4 cut(s) 183, 407, 2608, 2674
Hpy99I CGWCG 2 cut(s) 170, 2234
HpyCH4IV ACGT 9 cut(s) 306, 315, 1071, 1854, 2229, 2800, 3349, 3380, 4066
HpyF10VI GCNNNNNNNGC 6 cut(s) 53, 167, 183, 352, 3472, 3557
HpyF3I CTNAG 5 cut(s) 1061, 1501, 3957, 3984, 4167
HpySE526I ACGT 9 cut(s) 306, 315, 1071, 1854, 2229, 2800, 3349, 3380, 4066
Hsp92I GRCGYC 4 cut(s) 306, 393, 411, 540
HspAI GCGC 2 cut(s) 411, 2917
KasI GGCGCC 1 cut(s) 410
Kpn2I TCCGGA 1 cut(s) 2673
KpnI GGTACC 1 cut(s) 2007
KroI GCCGGC 2 cut(s) 182, 2607
KroNI GCCGGC 2 cut(s) 184, 2609
Ksp22I TGATCA 1 cut(s) 1561
KspAI GTTAAC 2 cut(s) 1407, 1765
Lsp1109I GCAGC 7 cut(s) 762, 2221, 2418, 2452, 2528, 2939, 4027
MaeI CTAG 7 cut(s) 20, 506, 618, 1484, 2345, 3161, 4052
MaeII ACGT 9 cut(s) 306, 315, 1071, 1854, 2229, 2800, 3349, 3380, 4066
MbiI CCGCTC 2 cut(s) 290, 346
MfeI CAATTG 2 cut(s) 250, 3756
MflI RGATCY 6 cut(s) 1017, 1486, 2546, 2581, 3223, 3921
MhlI GDGCHC 5 cut(s) 1115, 1502, 2453, 2713, 3148
MlsI TGGCCA 4 cut(s) 874, 1190, 3138, 3929
MluNI TGGCCA 4 cut(s) 874, 1190, 3138, 3929
Mly113I GGCGCC 1 cut(s) 411
MlyI GAGTC 2 cut(s) 642, 3212
MmeI TCCRAC 3 cut(s) 616, 3274, 3363
Mox20I TGGCCA 4 cut(s) 874, 1190, 3138, 3929
Mph1103I ATGCAT 4 cut(s) 2287, 3180, 3373, 3616
MroI TCCGGA 1 cut(s) 2673
MroNI GCCGGC 2 cut(s) 182, 2607
MroXI GAANNNNTTC 4 cut(s) 1431, 2861, 3840, 4158
MscI TGGCCA 4 cut(s) 874, 1190, 3138, 3929
MslI CAYNNNNRTG 2 cut(s) 474, 2478
Msp20I TGGCCA 4 cut(s) 874, 1190, 3138, 3929
MspA1I CMGCKG 3 cut(s) 2930, 3956, 4206
MspI CCGG 4 cut(s) 183, 407, 2608, 2674
MspR9I CCNGG 6 cut(s) 407, 408, 934, 1731, 3033, 4037
MunI CAATTG 2 cut(s) 250, 3756
Mva1269I GAATGC 2 cut(s) 170, 1042
MvaI CCWGG 4 cut(s) 934, 1731, 3033, 4037
MwoI GCNNNNNNNGC 6 cut(s) 53, 167, 183, 352, 3472, 3557
NaeI GCCGGC 2 cut(s) 184, 2609
NarI GGCGCC 1 cut(s) 411
NciI CCSGG 2 cut(s) 407, 408
NcoI CCATGG 1 cut(s) 1275
NdeI CATATG 1 cut(s) 688
NgoMIV GCCGGC 2 cut(s) 182, 2607
NlaIV GGNNCC 8 cut(s) 257, 336, 412, 1114, 2005, 3030, 3172, 3963
NmuCI GTSAC 8 cut(s) 1465, 1525, 1616, 1924, 2247, 2509, 3265, 4105
NsiI ATGCAT 4 cut(s) 2287, 3180, 3373, 3616
NspI RCATGY 8 cut(s) 842, 2289, 2309, 2535, 2607, 3120, 3182, 3819
NspV TTCGAA 1 cut(s) 2791
OliI CACNNNNGTG 1 cut(s) 474
PaeI GCATGC 2 cut(s) 2289, 2607
PagI TCATGA 2 cut(s) 1558, 2985
PceI AGGCCT 1 cut(s) 264
PciI ACATGT 1 cut(s) 3116
PcsI WCGNNNNNNNCGW 3 cut(s) 312, 372, 2797
PctI GAATGC 2 cut(s) 170, 1042
PdiI GCCGGC 2 cut(s) 184, 2609
PdmI GAANNNNTTC 4 cut(s) 1431, 2861, 3840, 4158
PfeI GAWTC 9 cut(s) 23, 599, 1099, 1385, 1982, 2734, 3003, 3063, 4083
PflMI CCANNNNNTGG 1 cut(s) 4067
PfoI TCCNGGA 1 cut(s) 3031
PkrI GCNGC 9 cut(s) 345, 777, 2236, 2433, 2467, 2518, 2929, 4042, 4205
Ple19I CGATCG 1 cut(s) 1543
PleI GAGTC 2 cut(s) 641, 3211
PluTI GGCGCC 1 cut(s) 414
PmaCI CACGTG 2 cut(s) 3350, 4067
PmlI CACGTG 2 cut(s) 3350, 4067
PpsI GAGTC 2 cut(s) 641, 3211
Ppu21I YACGTR 3 cut(s) 2801, 3350, 4067
PscI ACATGT 1 cut(s) 3116
PshBI ATTAAT 1 cut(s) 3231
Psp124BI GAGCTC 1 cut(s) 1502
Psp1406I AACGTT 1 cut(s) 3380
Psp6I CCWGG 4 cut(s) 932, 1729, 3031, 4035
PspCI CACGTG 2 cut(s) 3350, 4067
PspFI CCCAGC 1 cut(s) 1169
PspGI CCWGG 4 cut(s) 932, 1729, 3031, 4035
PspN4I GGNNCC 8 cut(s) 257, 336, 412, 1114, 2005, 3030, 3172, 3963
PspPI GGNCC 4 cut(s) 31, 255, 1805, 3171
PstI CTGCAG 2 cut(s) 1212, 2470
PstNI CAGNNNCTG 4 cut(s) 1646, 2699, 2930, 4142
PsuI RGATCY 6 cut(s) 1017, 1486, 2546, 2581, 3223, 3921
PvuI CGATCG 1 cut(s) 1543
PvuII CAGCTG 3 cut(s) 2930, 3956, 4206
RsaI GTAC 9 cut(s) 1029, 2005, 2208, 2567, 2688, 2698, 2952, 3688, 3797
RsaNI GTAC 9 cut(s) 1028, 2004, 2207, 2566, 2687, 2697, 2951, 3687, 3796
RseI CAYNNNNRTG 2 cut(s) 474, 2478
SacI GAGCTC 1 cut(s) 1502
SatI GCNGC 9 cut(s) 344, 776, 2235, 2432, 2466, 2517, 2928, 4041, 4204
Sau96I GGNCC 4 cut(s) 31, 255, 1805, 3171
SbfI CCTGCAGG 1 cut(s) 1212
SchI GAGTC 2 cut(s) 642, 3212
ScrFI CCNGG 6 cut(s) 407, 408, 934, 1731, 3033, 4037
SdaI CCTGCAGG 1 cut(s) 1212
SduI GDGCHC 5 cut(s) 1115, 1502, 2453, 2713, 3148
SfcI CTRYAG 8 cut(s) 1033, 1104, 1208, 1230, 2274, 2466, 2889, 2931
SfoI GGCGCC 1 cut(s) 412
SfuI TTCGAA 1 cut(s) 2791
SinI GGWCC 3 cut(s) 31, 255, 3171
SmaI CCCGGG 1 cut(s) 408
SmiMI CAYNNNNRTG 2 cut(s) 474, 2478
SmlI CTYRAG 2 cut(s) 2598, 2841
SmoI CTYRAG 2 cut(s) 2598, 2841
SnaBI TACGTA 1 cut(s) 2801
SpeI ACTAGT 1 cut(s) 3160
SphI GCATGC 2 cut(s) 2289, 2607
SrfI GCCCGGGC 1 cut(s) 408
Sse8387I CCTGCAGG 1 cut(s) 1212
SseBI AGGCCT 1 cut(s) 264
SsiI CCGC 8 cut(s) 54, 175, 237, 288, 344, 438, 770, 3466
SspDI GGCGCC 1 cut(s) 410
SspI AATATT 3 cut(s) 1401, 1660, 1708
SspMI CTAG 7 cut(s) 20, 506, 618, 1484, 2345, 3161, 4052
SstI GAGCTC 1 cut(s) 1502
StuI AGGCCT 1 cut(s) 264
StyD4I CCNGG 6 cut(s) 405, 406, 932, 1729, 3031, 4035
StyI CCWWGG 3 cut(s) 259, 1275, 3531
TaiI ACGT 9 cut(s) 309, 318, 1074, 1857, 2232, 2803, 3352, 3383, 4069
TaqI TCGA 9 cut(s) 165, 366, 760, 1817, 2713, 2791, 3212, 3420, 4081
TatI WGTACW 3 cut(s) 2565, 2686, 2950
TauI GCSGC 1 cut(s) 346
TfiI GAWTC 9 cut(s) 23, 599, 1099, 1385, 1982, 2734, 3003, 3063, 4083
TseFI GTSAC 8 cut(s) 1465, 1525, 1616, 1924, 2247, 2509, 3265, 4105
TseI GCWGC 8 cut(s) 775, 2234, 2431, 2465, 2516, 2927, 4040, 4203
Tsp45I GTSAC 8 cut(s) 1465, 1525, 1616, 1924, 2247, 2509, 3265, 4105
TspGWI ACGGA 4 cut(s) 44, 184, 509, 807
TspMI CCCGGG 1 cut(s) 406
Van91I CCANNNNNTGG 1 cut(s) 4067
VneI GTGCAC 2 cut(s) 2449, 3144
VpaK11BI GGWCC 3 cut(s) 31, 255, 3171
VspI ATTAAT 1 cut(s) 3231
XapI RAATTY 8 cut(s) 575, 889, 1532, 1553, 1795, 2857, 3150, 3839
XbaI TCTAGA 1 cut(s) 19
XceI RCATGY 8 cut(s) 842, 2289, 2309, 2535, 2607, 3120, 3182, 3819
XcmI CCANNNNNNNNNTGG 2 cut(s) 2965, 3762
XmaI CCCGGG 1 cut(s) 406
XmnI GAANNNNTTC 4 cut(s) 1431, 2861, 3840, 4158
XspI CTAG 7 cut(s) 20, 506, 618, 1484, 2345, 3161, 4052
ZraI GACGTC 1 cut(s) 307
Zsp2I ATGCAT 4 cut(s) 2287, 3180, 3373, 3616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.