Rh3AG313200

DNA ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0015031
Physical Location & Seq
Reverse (-)
39871411 .. 39874427
3017 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG313200.1

Sequence Viewer

Length: 1755 bp
ATGACTCGAGAAAGAGAGAAATATCGTTGGTGGCTCTCTTTGGCGAGCGGCGGCGACAAAAATTCTTGCTTGCTTCTCTATGGAAGCGGCAATAAATCTCTTCGTTTGCCTCTTTCTTCGGTTAGAGGAGGCGGTTTTGCTGATGTTGGGGGGATTGGAAATGGCATCTTGATCGACCATTTCTGGTGGGATGTTCCTATTAAGGGTGAGGATGGATCTGCTCTTGATCCGTCGATCTATCGCGGCGGCCAAATTCCTCTTGTTGGCAGTGGTGGTGCTTTGTGGCGGGTTTGTGTGCAACAGTATGGCGAATCTGGGCGAGGAGACGACTTCTTCTTCTTCTCCATGTCCTCGTCGTCTTTTGATGGTCCTAGCGGTGCAGATCTCGCGGTCGGTGCAGTTGGTTGGCTTTGGGTGTTGACAGCAGGGTATATGATCTTTGATGGTGGTGGCGGTAATTTGTCTCAGGTTGTTGACATTGGGACTGTTCTGGTGGCCACCACTGGTGCTTGCGACGGCGATGGAGGCAGGATAGAAGCCGACATGGAAAGAATTCCGAATGCTCTGTTCTTGATGACGGCTCTGGATTCTACGGACCTCTTCCTGACTGCCCTAACCGCCTTTTCCCACCCACCCAGACCCATACCCCGTCCCCTTGAACTCTCTCTCCCTCCAATTCCCTCCACATTCCCCCACTCCAAACTCATCCCCAGAACCCGTTTCGCCGTCGATGCATTCCGCTATGCCGGCGATTATTCCGTCTCTTACTTCCTCTCTCACTTCCACTCCGATCACTACGGCGGTCTCTCCCCCAATTGGTCCCAAGGCCTCGTCTTTTGCTCCCCCACCACCGCTCGTCTTCTCACAGACGTCCTCAACGTCTCTTCGCTTTTCGTGGTTCCTCTGCCGCTCGGACAGCCCCTTCTCATCGATGGATGCGAGGTCGTGCTTATTGACGCCAATCATTGCCCGGGCGCCGTTCAGTTCTTGTTCAAAGTTCCCGCCTCCAATGGAGACTTCCAGAGGTATGTTCACACTGGTGATTTTCGGTTTTCTGCTCCGATGAAGCTAGACCCTTTTCTCTGTAACTTTGTGGGTTCAGACGCCGTTTTCTTGGATACAACTTATTGCAATCCCAAATTCGTGTTTCCTTCACAAGAAGAATCTGTTGATTATATTTCTAGCTTGATAGAAAGAGTTGGAGGTGAACATGAAGTTTCTGTGAAAAGTGTTCTGTTCCTTGTTGCTACATATGTGATTGGGAAAGAGAAGATTTTGATAGAAATTGCTCGTAGATGTAAGCGTAAAGTGTATGTGGATGCTCGAAAAATGGCGGTGCTGCGTGTTTTGGGTTACGGAGACAGTGGGGTGTTTACGGAGGATGAATGTGAGAGTGATGTGCATGTTGTTGGGTGGAATGTGTTGGGTGAGACTTGGCCATATTTCAGGCCAAATTTTGTGAAAATGGAGGAGATTATGGTTGAGAGGGGGTATTCCAGGGTTGTAGGGTTTGTCCCTACTGGCTGGACTTATGAAGTCAAGCGTAACAAGTTCTCGGTGAGGTCAAAGGATGCTTTTGAGATCCATCTTGTACCCTACAGTGAGCATTCAAGCTATGATGAACTTAGAGAATACGTCAGGTTTTTGAAACCAAGGCGTGTGATTCCTACAGTGGGCTCCGATGTGGAAAAGATAGACAGTAAACATGCCAGTAAAATGCAAAAGCATTTTGCTGGGTTAATAATTAACCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000012 GO:0000726 GO:0002237 GO:0003006 GO:0003674 GO:0003824 GO:0003909 GO:0003910 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006266 GO:0006271 GO:0006273 GO:0006281 GO:0006302 GO:0006303 GO:0006304 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009314 GO:0009409 GO:0009411 GO:0009416 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009791 GO:0009845 GO:0009987 GO:0010033 GO:0010154 GO:0010225 GO:0010243 GO:0015074 GO:0016070 GO:0016874 GO:0016886 GO:0022414 GO:0022616 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0033554 GO:0034641 GO:0034645 GO:0035510 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044728 GO:0046483 GO:0046677 GO:0048316 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051103 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0065007 GO:0070013 GO:0070988 GO:0071704 GO:0080111 GO:0090304 GO:0090351 GO:0140097 GO:1901360 GO:1901576 GO:1901652 GO:1901698 GO:1901700 GO:1904975 GO:2000683 GO:2000685
KEGG Pathways
Metabolic & Signaling
ko03030 ko03410 ko03420 ko03430 map03030 map03410 map03420 map03430
Pfam Domains
Protein Families
DNA_ligase_A_C DNA_ligase_A_M DNA_ligase_A_N DRMBL

Protein Analysis

584

Amino Acids

64.61

Weight (kDa)

5.99

Isoelectric Point (pI)

36.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DRMBL PF07522 456 - 559 2.5e-21 DNA repair metallo-beta-lactamase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015031)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66730
fragaria_vesca FvH4_1g29000 FvH4_1g29000 FvH4_1g29000
malus_domestica MD00G1054100.v1.1
prunus_persica Prupe.3G150300_v2.0.a1
pyrus_communis pycom17g25560
rosa_chinensis RchiOBHm_Chr3g0493691 RchiOBHm_Chr3g0493951
rosa_laevigata RLG00000022694
rosa_roxburghii Rroxscaffold_6G00391370
rosa_rugosa Rorug03G0263400 Rorug03G0263500 Rorug03G0263600
rosa_samantha Rh3AG312500 Rh3AG313200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 873
AccB1I GGYRCC 1 cut(s) 974
AccBSI CCGCTC 3 cut(s) 48, 854, 910
AccII CGCG 2 cut(s) 243, 389
AclWI GGATC 3 cut(s) 221, 223, 1576
AcoI YGGCCR 3 cut(s) 247, 495, 1436
AcsI RAATTY 5 cut(s) 61, 252, 552, 1139, 1453
AcyI GRCGYC 4 cut(s) 870, 957, 975, 1104
AfaI GTAC 1 cut(s) 1593
AfiI CCNNNNNNNGG 4 cut(s) 203, 263, 816, 1673
AgsI TTSAA 4 cut(s) 659, 994, 1611, 1648
AjnI CCWGG 1 cut(s) 1496
AjuI GAANNNNNNNTTGG 2 cut(s) 1001, 1033
AleI CACNNNNGTG 1 cut(s) 1038
AloI GAACNNNNNNTCC 2 cut(s) 651, 683
AluBI AGCT 3 cut(s) 1069, 1185, 1614
AluI AGCT 3 cut(s) 1069, 1185, 1614
Alw26I GTCTC 8 cut(s) 318, 468, 766, 809, 886, 1008, 1353, 1424
AlwI GGATC 3 cut(s) 221, 223, 1576
Ama87I CYCGRG 2 cut(s) 6, 970
AoxI GGCC 5 cut(s) 247, 495, 826, 1436, 1448
ApeKI GCWGC 1 cut(s) 1339
ApoI RAATTY 5 cut(s) 61, 252, 552, 1139, 1453
Asp700I GAANNNNTTC 1 cut(s) 552
AspLEI GCGC 1 cut(s) 977
AspS9I GGNCC 3 cut(s) 368, 595, 819
AsuC2I CCSGG 2 cut(s) 971, 972
AsuHPI GGTGA 5 cut(s) 218, 1052, 1217, 1439, 1570
AvaI CYCGRG 2 cut(s) 6, 970
AvaII GGWCC 3 cut(s) 368, 595, 819
BalI TGGCCA 2 cut(s) 497, 1438
BanI GGYRCC 1 cut(s) 974
BanII GRGCYC 1 cut(s) 1679
BbsI GAAGAC 1 cut(s) 851
BbvI GCAGC 1 cut(s) 1326
BccI CCATC 6 cut(s) 206, 359, 437, 515, 926, 1593
BceAI ACGGC 6 cut(s) 532, 594, 710, 814, 962, 1091
BcgI CGANNNNNNTGC 2 cut(s) 154, 188
BciT130I CCWGG 1 cut(s) 1498
BciVI GTATCC 1 cut(s) 1111
BcnI CCSGG 2 cut(s) 971, 972
BcoDI GTCTC 8 cut(s) 318, 468, 766, 809, 886, 1008, 1353, 1424
BfaI CTAG 3 cut(s) 372, 1070, 1182
BfmI CTRYAG 2 cut(s) 1597, 1668
BfoI RGCGCY 1 cut(s) 978
BfuI GTATCC 1 cut(s) 1111
BglII AGATCT 1 cut(s) 382
BisI GCNGC 7 cut(s) 49, 52, 88, 244, 247, 908, 1340
BlsI GCNGC 7 cut(s) 50, 53, 89, 245, 248, 909, 1341
Bme1390I CCNGG 3 cut(s) 971, 972, 1498
Bme18I GGWCC 3 cut(s) 368, 595, 819
BmeT110I CYCGRG 2 cut(s) 6, 970
BmgT120I GGNCC 3 cut(s) 368, 595, 819
BmiI GGNNCC 4 cut(s) 821, 900, 976, 1678
BmrFI CCNGG 3 cut(s) 971, 972, 1498
BmsI GCATC 5 cut(s) 174, 721, 926, 1309, 1561
BpiI GAAGAC 1 cut(s) 851
BpuMI CCSGG 2 cut(s) 971, 972
Bsa29I ATCGAT 1 cut(s) 930
BsaHI GRCGYC 4 cut(s) 870, 957, 975, 1104
BsaI GGTCTC 1 cut(s) 809
BsaJI CCNNGG 4 cut(s) 823, 970, 1497, 1652
BsaXI ACNNNNNCTCC 4 cut(s) 651, 681, 770, 800
Bsc4I CCNNNNNNNGG 4 cut(s) 203, 263, 816, 1673
Bse118I RCCGGY 1 cut(s) 746
Bse1I ACTGG 4 cut(s) 508, 1042, 1525, 1710
Bse3DI GCAATG 1 cut(s) 964
BseBI CCWGG 1 cut(s) 1498
BseCI ATCGAT 1 cut(s) 930
BseDI CCNNGG 4 cut(s) 823, 970, 1497, 1652
BseGI GGATG 7 cut(s) 196, 217, 705, 941, 1324, 1387, 1576
BseLI CCNNNNNNNGG 4 cut(s) 203, 263, 816, 1673
BseMI GCAATG 1 cut(s) 964
BseMII CTCAG 1 cut(s) 479
BseNI ACTGG 4 cut(s) 508, 1042, 1525, 1710
BseRI GAGGAG 3 cut(s) 141, 336, 1484
BseXI GCAGC 1 cut(s) 1326
BseYI CCCAGC 1 cut(s) 1733
BsgI GTGCAG 2 cut(s) 399, 417
Bsh1236I CGCG 2 cut(s) 243, 389
Bsh1285I CGRYCG 1 cut(s) 393
BshFI GGCC 5 cut(s) 249, 497, 828, 1438, 1450
BshNI GGYRCC 1 cut(s) 974
BshVI ATCGAT 1 cut(s) 930
BsiEI CGRYCG 1 cut(s) 393
BsiHKCI CYCGRG 2 cut(s) 6, 970
BsiSI CCGG 2 cut(s) 747, 971
BslFI GGGAC 4 cut(s) 496, 636, 805, 1499
BslI CCNNNNNNNGG 4 cut(s) 203, 263, 816, 1673
BsmAI GTCTC 8 cut(s) 318, 468, 766, 809, 886, 1008, 1353, 1424
BsmBI CGTCTC 3 cut(s) 318, 766, 886
BsmFI GGGAC 4 cut(s) 496, 636, 805, 1499
BsmI GAATGC 3 cut(s) 565, 734, 1606
BsnI GGCC 5 cut(s) 249, 497, 828, 1438, 1450
Bso31I GGTCTC 1 cut(s) 809
BsoBI CYCGRG 2 cut(s) 6, 970
Bsp1286I GDGCHC 1 cut(s) 1679
Bsp143I GATC 8 cut(s) 171, 215, 226, 234, 382, 435, 790, 1581
BspANI GGCC 5 cut(s) 249, 497, 828, 1438, 1450
BspCNI CTCAG 1 cut(s) 478
BspDI ATCGAT 1 cut(s) 930
BspFNI CGCG 2 cut(s) 243, 389
BspLI GGNNCC 4 cut(s) 821, 900, 976, 1678
BspPI GGATC 3 cut(s) 221, 223, 1576
BspT107I GGYRCC 1 cut(s) 974
BspTNI GGTCTC 1 cut(s) 809
BsrBI CCGCTC 3 cut(s) 48, 854, 910
BsrDI GCAATG 1 cut(s) 964
BsrFI RCCGGY 1 cut(s) 746
BsrI ACTGG 4 cut(s) 508, 1042, 1525, 1710
BssAI RCCGGY 1 cut(s) 746
BssECI CCNNGG 4 cut(s) 823, 970, 1497, 1652
BssMI GATC 8 cut(s) 171, 215, 226, 234, 382, 435, 790, 1581
BssNI GRCGYC 4 cut(s) 870, 957, 975, 1104
BssT1I CCWWGG 2 cut(s) 823, 1652
Bst2UI CCWGG 1 cut(s) 1498
Bst4CI ACNGT 7 cut(s) 303, 487, 1364, 1601, 1672, 1700, 1751
Bst6I CTCTTC 3 cut(s) 105, 605, 889
BstACI GRCGYC 4 cut(s) 870, 957, 975, 1104
BstC8I GCNNGC 4 cut(s) 46, 71, 511, 748
BstDEI CTNAG 2 cut(s) 465, 1625
BstF5I GGATG 7 cut(s) 196, 217, 705, 941, 1324, 1387, 1576
BstFNI CGCG 2 cut(s) 243, 389
BstH2I RGCGCY 1 cut(s) 978
BstHHI GCGC 1 cut(s) 977
BstKTI GATC 8 cut(s) 174, 218, 229, 237, 385, 438, 793, 1584
BstMAI GTCTC 8 cut(s) 318, 468, 766, 809, 886, 1008, 1353, 1424
BstMBI GATC 8 cut(s) 171, 215, 226, 234, 382, 435, 790, 1581
BstMCI CGRYCG 1 cut(s) 393
BstMWI GCNNNNNNNGC 7 cut(s) 386, 395, 525, 617, 731, 747, 916
BstNI CCWGG 1 cut(s) 1498
BstNSI RCATGY 2 cut(s) 1406, 1709
BstSCI CCNGG 3 cut(s) 969, 970, 1496
BstSFI CTRYAG 2 cut(s) 1597, 1668
BstUI CGCG 2 cut(s) 243, 389
BstV1I GCAGC 1 cut(s) 1326
BstV2I GAAGAC 1 cut(s) 851
BstX2I RGATCY 3 cut(s) 215, 382, 1581
BstYI RGATCY 3 cut(s) 215, 382, 1581
Bsu15I ATCGAT 1 cut(s) 930
BsuI GTATCC 1 cut(s) 1111
BsuRI GGCC 5 cut(s) 249, 497, 828, 1438, 1450
BsuTUI ATCGAT 1 cut(s) 930
BtgZI GCGATG 1 cut(s) 534
BtsCI GGATG 7 cut(s) 196, 217, 705, 941, 1324, 1387, 1576
BtsI GCAGTG 1 cut(s) 274
BtsIMutI CAGTG 6 cut(s) 274, 501, 1035, 1369, 1606, 1677
Cac8I GCNNGC 4 cut(s) 46, 71, 511, 748
CfoI GCGC 1 cut(s) 977
Cfr10I RCCGGY 1 cut(s) 746
Cfr13I GGNCC 3 cut(s) 368, 595, 819
Cfr9I CCCGGG 1 cut(s) 970
ClaI ATCGAT 1 cut(s) 930
CseI GACGC 2 cut(s) 965, 1112
Csp6I GTAC 1 cut(s) 1592
CviAII CATG 5 cut(s) 346, 544, 1211, 1403, 1706
CviQI GTAC 1 cut(s) 1592
DdeI CTNAG 2 cut(s) 465, 1625
DinI GGCGCC 1 cut(s) 976
DpnI GATC 8 cut(s) 173, 217, 228, 236, 384, 437, 792, 1583
DpnII GATC 8 cut(s) 171, 215, 226, 234, 382, 435, 790, 1581
EaeI YGGCCR 3 cut(s) 247, 495, 1436
Eam1104I CTCTTC 3 cut(s) 105, 605, 889
EarI CTCTTC 3 cut(s) 105, 605, 889
Eco130I CCWWGG 2 cut(s) 823, 1652
Eco147I AGGCCT 1 cut(s) 828
Eco24I GRGCYC 1 cut(s) 1679
Eco31I GGTCTC 1 cut(s) 809
Eco47I GGWCC 3 cut(s) 368, 595, 819
Eco88I CYCGRG 2 cut(s) 6, 970
EcoRI GAATTC 1 cut(s) 552
EcoRII CCWGG 1 cut(s) 1496
EcoT14I CCWWGG 2 cut(s) 823, 1652
EcoT22I ATGCAT 1 cut(s) 736
EcoT38I GRGCYC 1 cut(s) 1679
EgeI GGCGCC 1 cut(s) 976
EheI GGCGCC 1 cut(s) 976
ErhI CCWWGG 2 cut(s) 823, 1652
Esp3I CGTCTC 3 cut(s) 318, 766, 886
FaeI CATG 5 cut(s) 349, 547, 1214, 1406, 1709
FaqI GGGAC 4 cut(s) 496, 636, 805, 1499
FatI CATG 5 cut(s) 345, 543, 1210, 1402, 1705
FauI CCCGC 2 cut(s) 279, 1009
FauNDI CATATG 1 cut(s) 1252
Fnu4HI GCNGC 7 cut(s) 49, 52, 88, 244, 247, 908, 1340
FokI GGATG 7 cut(s) 203, 224, 692, 948, 1331, 1394, 1583
FriOI GRGCYC 1 cut(s) 1679
Fsp4HI GCNGC 7 cut(s) 49, 52, 88, 244, 247, 908, 1340
FspBI CTAG 3 cut(s) 372, 1070, 1182
GlaI GCGC 1 cut(s) 976
GluI GCNGC 7 cut(s) 49, 52, 88, 244, 247, 908, 1340
GsaI CCCAGC 1 cut(s) 1737
HaeII RGCGCY 1 cut(s) 978
HaeIII GGCC 5 cut(s) 249, 497, 828, 1438, 1450
HapII CCGG 2 cut(s) 747, 971
HgaI GACGC 2 cut(s) 965, 1112
HhaI GCGC 1 cut(s) 977
Hin1I GRCGYC 4 cut(s) 870, 957, 975, 1104
Hin1II CATG 5 cut(s) 349, 547, 1214, 1406, 1709
Hin6I GCGC 1 cut(s) 975
HinP1I GCGC 1 cut(s) 975
HincII GTYRAC 2 cut(s) 420, 475
HindII GTYRAC 2 cut(s) 420, 475
HinfI GANTC 5 cut(s) 4, 311, 587, 1163, 1663
HpaII CCGG 2 cut(s) 747, 971
HphI GGTGA 5 cut(s) 218, 1052, 1217, 1439, 1570
Hpy166II GTNNAC 6 cut(s) 420, 475, 1033, 1208, 1374, 1703
Hpy188I TCNGA 6 cut(s) 558, 790, 914, 1062, 1102, 1681
Hpy188III TCNNGA 7 cut(s) 8, 169, 224, 571, 584, 604, 1021
Hpy8I GTNNAC 6 cut(s) 420, 475, 1033, 1208, 1374, 1703
Hpy99I CGWCG 4 cut(s) 235, 358, 518, 731
HpyAV CCTTC 2 cut(s) 932, 1161
HpyCH4III ACNGT 7 cut(s) 303, 487, 1364, 1601, 1672, 1700, 1751
HpyCH4IV ACGT 3 cut(s) 870, 879, 1635
HpyCH4V TGCA 7 cut(s) 298, 380, 398, 734, 1131, 1402, 1720
HpyF10VI GCNNNNNNNGC 7 cut(s) 386, 395, 525, 617, 731, 747, 916
HpyF3I CTNAG 2 cut(s) 465, 1625
HpySE526I ACGT 3 cut(s) 870, 879, 1635
Hsp92I GRCGYC 4 cut(s) 870, 957, 975, 1104
Hsp92II CATG 5 cut(s) 349, 547, 1214, 1406, 1709
HspAI GCGC 1 cut(s) 975
KasI GGCGCC 1 cut(s) 974
KroI GCCGGC 1 cut(s) 746
KroNI GCCGGC 1 cut(s) 748
Kzo9I GATC 8 cut(s) 171, 215, 226, 234, 382, 435, 790, 1581
LmnI GCTCC 3 cut(s) 845, 1063, 1682
Lsp1109I GCAGC 1 cut(s) 1326
LweI GCATC 5 cut(s) 174, 721, 926, 1309, 1561
MaeI CTAG 3 cut(s) 372, 1070, 1182
MaeII ACGT 3 cut(s) 870, 879, 1635
MaeIII GTNAC 3 cut(s) 1085, 1352, 1544
MalI GATC 8 cut(s) 173, 217, 228, 236, 384, 437, 792, 1583
MbiI CCGCTC 3 cut(s) 48, 854, 910
MboI GATC 8 cut(s) 171, 215, 226, 234, 382, 435, 790, 1581
MfeI CAATTG 1 cut(s) 814
MflI RGATCY 3 cut(s) 215, 382, 1581
MhlI GDGCHC 1 cut(s) 1679
MlsI TGGCCA 2 cut(s) 497, 1438
MluNI TGGCCA 2 cut(s) 497, 1438
Mly113I GGCGCC 1 cut(s) 975
MmeI TCCRAC 1 cut(s) 1180
Mox20I TGGCCA 2 cut(s) 497, 1438
Mph1103I ATGCAT 1 cut(s) 736
MroNI GCCGGC 1 cut(s) 746
MroXI GAANNNNTTC 1 cut(s) 552
MscI TGGCCA 2 cut(s) 497, 1438
MseI TTAA 4 cut(s) 201, 1739, 1746, 1753
MslI CAYNNNNRTG 1 cut(s) 1038
Msp20I TGGCCA 2 cut(s) 497, 1438
MspI CCGG 2 cut(s) 747, 971
MspR9I CCNGG 3 cut(s) 971, 972, 1498
MunI CAATTG 1 cut(s) 814
Mva1269I GAATGC 3 cut(s) 565, 734, 1606
MvaI CCWGG 1 cut(s) 1498
MvnI CGCG 2 cut(s) 243, 389
MwoI GCNNNNNNNGC 7 cut(s) 386, 395, 525, 617, 731, 747, 916
NaeI GCCGGC 1 cut(s) 748
NarI GGCGCC 1 cut(s) 975
NciI CCSGG 2 cut(s) 971, 972
NdeI CATATG 1 cut(s) 1252
NdeII GATC 8 cut(s) 171, 215, 226, 234, 382, 435, 790, 1581
NgoMIV GCCGGC 1 cut(s) 746
NlaIII CATG 5 cut(s) 349, 547, 1214, 1406, 1709
NlaIV GGNNCC 4 cut(s) 821, 900, 976, 1678
NsiI ATGCAT 1 cut(s) 736
NspI RCATGY 2 cut(s) 1406, 1709
OliI CACNNNNGTG 1 cut(s) 1038
PaeR7I CTCGAG 1 cut(s) 6
PceI AGGCCT 1 cut(s) 828
PcsI WCGNNNNNNNCGW 2 cut(s) 876, 936
PctI GAATGC 3 cut(s) 565, 734, 1606
PdiI GCCGGC 1 cut(s) 748
PdmI GAANNNNTTC 1 cut(s) 552
PfeI GAWTC 4 cut(s) 311, 587, 1163, 1663
PkrI GCNGC 7 cut(s) 50, 53, 89, 245, 248, 909, 1341
PluTI GGCGCC 1 cut(s) 978
Psp6I CCWGG 1 cut(s) 1496
PspFI CCCAGC 1 cut(s) 1733
PspGI CCWGG 1 cut(s) 1496
PspN4I GGNNCC 4 cut(s) 821, 900, 976, 1678
PspPI GGNCC 3 cut(s) 368, 595, 819
PsuI RGATCY 3 cut(s) 215, 382, 1581
RsaI GTAC 1 cut(s) 1593
RsaNI GTAC 1 cut(s) 1592
RseI CAYNNNNRTG 1 cut(s) 1038
SaqAI TTAA 4 cut(s) 201, 1739, 1746, 1753
SatI GCNGC 7 cut(s) 49, 52, 88, 244, 247, 908, 1340
Sau3AI GATC 8 cut(s) 171, 215, 226, 234, 382, 435, 790, 1581
Sau96I GGNCC 3 cut(s) 368, 595, 819
ScrFI CCNGG 3 cut(s) 971, 972, 1498
SduI GDGCHC 1 cut(s) 1679
SfaNI GCATC 5 cut(s) 174, 721, 926, 1309, 1561
SfcI CTRYAG 2 cut(s) 1597, 1668
SfoI GGCGCC 1 cut(s) 976
Sfr274I CTCGAG 1 cut(s) 6
SinI GGWCC 3 cut(s) 368, 595, 819
SlaI CTCGAG 1 cut(s) 6
SmaI CCCGGG 1 cut(s) 972
SmiMI CAYNNNNRTG 1 cut(s) 1038
SmlI CTYRAG 1 cut(s) 6
SmoI CTYRAG 1 cut(s) 6
SrfI GCCCGGGC 1 cut(s) 972
SseBI AGGCCT 1 cut(s) 828
SspDI GGCGCC 1 cut(s) 974
SspMI CTAG 3 cut(s) 372, 1070, 1182
StuI AGGCCT 1 cut(s) 828
StyD4I CCNGG 3 cut(s) 969, 970, 1496
StyI CCWWGG 2 cut(s) 823, 1652
TaaI ACNGT 7 cut(s) 303, 487, 1364, 1601, 1672, 1700, 1751
TaiI ACGT 3 cut(s) 873, 882, 1638
TaqI TCGA 6 cut(s) 7, 174, 233, 729, 930, 1324
TauI GCSGC 6 cut(s) 51, 54, 90, 246, 249, 910
TfiI GAWTC 4 cut(s) 311, 587, 1163, 1663
Tru1I TTAA 4 cut(s) 201, 1739, 1746, 1753
Tru9I TTAA 4 cut(s) 201, 1739, 1746, 1753
TscAI CASTG 6 cut(s) 274, 508, 1042, 1369, 1606, 1677
TseI GCWGC 1 cut(s) 1339
TspDTI ATGAA 5 cut(s) 1079, 1227, 1398, 1548, 1635
TspGWI ACGGA 5 cut(s) 219, 608, 748, 1371, 1391
TspMI CCCGGG 1 cut(s) 970
TspRI CASTG 6 cut(s) 274, 508, 1042, 1369, 1606, 1677
VpaK11BI GGWCC 3 cut(s) 368, 595, 819
XapI RAATTY 5 cut(s) 61, 252, 552, 1139, 1453
XceI RCATGY 2 cut(s) 1406, 1709
XhoI CTCGAG 1 cut(s) 6
XmaI CCCGGG 1 cut(s) 970
XmnI GAANNNNTTC 1 cut(s) 552
XspI CTAG 3 cut(s) 372, 1070, 1182
ZraI GACGTC 1 cut(s) 871
Zsp2I ATGCAT 1 cut(s) 736
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.