FvH4_1g29000

DNA ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0015031
Physical Location & Seq
Forward (+)
21433605 .. 21444140
10536 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g29000.t2

Sequence Viewer

Length: 4170 bp
ATGGCGGTTCTAGATTCTACGGACCTCTACCTGACAGCCCTAACAACCTTTTCACACCCACACCCGCCCCCACCTCAACTCTCTCTCCCTACACTTCCCCCCACATTCCCCCACTCCAAACTCATTCCCAGAACCCGTTTCGCCGTCGACGCTTTCCGCTACGCCGGCGATTACTCCGTCTCTTACTTCCTCTCTCACTTCCACTCGGATCACTACGGCGGTCTCTCTCCCAGTTGGTCTAAGGGCCTCGTGTTCTGCTCCCCCACCACCGCCCGTCTCCTCACACAGGTCCTCCGCGTCTCTTCTCTCTTTGTCGTTGCCCTCCCACTCCGACAACCCCTTGTCATCGATGGATGCGAGGTTGTGCTTGTTGACGCCAATCACTGTCCGGGTGCCGTTCAGTTCTTGTTCCAAGTCCCCCTTCCCGGCGGTGCCCAACTCTCCGAGAGGTATCTTCATACTGGTGATTTTCGATTTTCTCCTTGCATGAAGTCGGACCCTTTTCTCTCTACATTTGTGGGCTGCGAAGCCGTTTTCTTGGATACCACTTATTGTAATCCCAAATTCGTGTTTCCTTCGCAACAAGAATCTGTTGATTATGTTTGTCGCGTGATCCAGACGGAGACAGTTGTAGGCCAACCCAAAACTGTTCTCTTCCTTGTTGCTACTTATGTTATTGGGAAAGAGAAGATTCTGTTGGAAATTGCTCGTAGGTGTAACCGCAAAGTGCATGTGGATGCTCGGAAAATGGCCGTCTTGCGCGTCTTGGGTTTCGGAGACAGTGGGGTGTTTACGGAGGATGAATGTGAGACTGATGTGCATGTTGTTGGGTGGAACGTCTTGGGTGACACTTGGCCATATTTCCGGCCTAATTTTGTGAAAATAGAGGAGATTATGGCTCATAAGGGGTATTCCAGGGTTGTAGGCTTTGTCCCTACTGGCTGGACTTATGAAGTGAAGCGTAATAAGTTTTCGGTAAGGTCAAAGGATGCTCTTGAGATCCATCTTGTGCCCTACAGTGAACATTCAAACTATGATGAACTTAGAGAATACGTGCGGTTTTTGAAACCAAAACGCGTGATTCCTACTGTGGGTTCCGATGTCGAGAAGATTGACAGTAAACATGCCGGTAAAATGCAAAAGCATTTTGCTGGGTTGATCGATGAAATGGCTAACAAAAAAGAGTTCTTGAGGGGTTTTCATTGTGGGTCTACAGAAGTAGGTGGGAAGGTTGACAGTGATGCCAATGATTGCCCCATGGATGGGCAGTATCTGGATGAAAAGGCATCAACTGATACTAATGTTGGCGCTCCCATTCAATTGTTCTCTCCTCTCCAAGAACCTGATTCACAAACTCCAATGCTGTTAACTGATGACAAAGAGGAGGAAATCATTCAAGAACTTCGTAATTGTCTGCCTTCCTGGGTCACACGCCAGCAAATGCTAGAGCTGATTGGGAGCTCAGGAGGCGACATTGTTGAAGCAGTCAGTAAATTTTATGATCGTGAAACAGAATTTCGTGGTCAAGGGATTGCCTCTGCAACTGCTGTATCTGTATCCGAGACCAGCAAACTGTGTGACACTGCAACGCCGACAAAAGCAGGTTCTGTTCATGCAAATATTGACGTTTCTTCAAGCCGAGATCATATTTCACCCAACCCAAGTAACATCATCATTAAAAGTGGCATTTCTCCTGGCAAAAGAGGGAAGAAGATTAGTAATAAAGTAAACAAGAAACTCAAGTTACAGCCGAAGTTGGAGTCATGTGGGCCAAAACAATCAATCATAACAAGGTTTTTTAGTAAAGTTTTGCCTGATGTTACAGCAAGTGGAGAGACTGGATCCATGGGTGAACAAAACCCTAAAGATAAAAATTTGCCCGATCATGGCACCCAACCCTACAAGGATGCAGTAGATCAATTTCTTCAGATAATAGATGGCAATGAATCATTAAAAAGCTATGCTGATAGAGTTATTCGAAAGGCAAATGGGGATATTAGTAGGGCAGTGGATATACATTACTGTAACGAAGGCAAGTCTGGTGAGAATGAAATGGAGTTAGTGGCTGAGGCAAACTCAGTTCAGTCCAACAGTTGTGTGGACAACTATTCCGCTGACCAAAAGATAATTGAATTAGGGAAGACAGGACTGTTAGCTGATTCATCTGTTCTGCTTTCATCACCAGATAATATAGATGCAACTTCTGTTTCACTACCGCCAGAAAAATACAACCCTGTAGAGCATGCATGCTGGAGTAATGGACAACATGCTCCTTATTTACATCTTGCACGAACCTTTGACTTACTTGAGAATGAAAAAGGCAAGATAAAGGCTACATCTATGCTCTGCAATATGTTCAGAAGTTTGCTTGCCTTGTCTCCTGATGATGTGCTGCCTTCAGTTTATTTGTGCACCAATAAGATAGCTGCAGACCATAAAAATGTGGAGCTGAACATTGGTGGAAGTTTGGTTACATCAGCACTGGAGGATGCATGCGGGACAAGTAGATCCAAAATCAGGGATATGTACAATGAATTGGGAGATCTTGGTGATGTTGCTCAAGCATGCCGACAGACACAGACTTTACTTGCCCCTCCTTCACCACTTCTAATAAAAGATGTATTTTTGGCGCTCTGGAAGATAAGTGTACAAACAGGTAGTGGAAGCAGTGCTCGAAAGAGAAGCCTGATTGTGAATCTCATGCGTTCTTGCAGAGAGAAAGAGATGAAGTTCCTTGTCAGAACACTGGTTCGGAACTTACGTATTGGGGCAATGATGAAAACTGTTCTACCTGCTCTGGCTCAAGCTGTTGTTCTGAATTCCTTCCATAGCTGTAACCACAAAGGAACTATAGAAAGTTTGATGGATAAGCTTCAGCGTCATTCAGCAGCTGTAGTAGAGGCATACAATGTACTGCCCAGTTTGGATGTGGTCATTCCTTCTCTCATGAAAAGAGGCATTGGATTCTCTTCATCAACTTTGTCAATGGTTCCAGGAATACCAATCAAGCCAATGCTTGCAAGAATCACAAATGGTGTTCAGCAAACACTGAAGCTGTTTGAAAATAAAGCTTTTACATGTGAATACAAATATGATGGCCAGCGTGCACAAATTCATAAACTAGTTGATGGGTCCGTGCATATTTTTTCACGAAATGGGGACGAGTCGACTTCGAGATTTCCAGATCTGATTAATATAATCAATCAATCTTGTAAACCTGATGCATTAACTTTCATACTGGATGGAGAGGTTGTCGGAGTTGATAGGAAGAATGGGTGCAGGCTAATGTCCTTCCAAGAATTATCTTCACGTGGGAGAGGGAGCAGAGATGCATCAATAACATTGGATAGCATAAAGGTCGACATTTGCGTATTTGTCTTCGACATCATGTTTGCCAATGGACAACAGTTGTTAAGCTTTCCTCTGAGGAGAAGACGAAAGTATTTGAAAGAAATGTTTTACGATGAGAAGTTGGGTTACTTCGAATATGCCAAGGAAATGACAGTTGAGGCAGAGGATGCTTGTTTAGCCAGTGAAGCGACATTAGCAAAAATGAACTCTTTCCTTGAAAATGCATTTGTTTCCTCATGTGAAGGGATTATGGTGAAATGTTTAGATGTTGATGCTGGATATTTGCCATCAAAGCGTATGGATACTTGGTTAAAGGTCAAGCGAGATTACATAGAGGGATTGCGTGATTCACTTGATTTGGTCCCAATTGGAGCTTGGCATGGAAACGGGAGAAAGGCAGGCTGGTACAGTCCATTTCTCATGGCATGTTACAACCCTGAAACTGAAGAACTTCAAAGCGTCTGTCGTGTTATGTCTGGGTTTTCAGATTTATTCTATACAGAGATGAAAAGTTTTTATTCTGGGGACAAGATCTTGGCCAAAAAACCACCTTACTATAGAACAGCTGAGGTGCCTGATATGTGGTTCTCTCCAGAGCTTGTTTGGGAAATAAGAGGTGCAGACTTTACAGTGTCTCCAGTTCACCAGGCCGCTTTGGGTTTAGTTCATCCATCACGTGGCATTTCCATTCGATTCCCGAGATACATTCGCACTGTGACTGATAGAAAACCAGAGGAATGTAGCACAGCTTCTGATATAGCTGAAATGTTTCATTCTCAGACGAGGAAGATGGACGTGGCTTCTGAAGATGGGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000012 GO:0000726 GO:0002237 GO:0003006 GO:0003674 GO:0003824 GO:0003909 GO:0003910 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006266 GO:0006271 GO:0006273 GO:0006281 GO:0006302 GO:0006303 GO:0006304 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009314 GO:0009409 GO:0009411 GO:0009416 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009791 GO:0009845 GO:0009987 GO:0010033 GO:0010154 GO:0010225 GO:0010243 GO:0015074 GO:0016070 GO:0016874 GO:0016886 GO:0022414 GO:0022616 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0033554 GO:0034641 GO:0034645 GO:0035510 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044728 GO:0046483 GO:0046677 GO:0048316 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051103 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0065007 GO:0070013 GO:0070988 GO:0071704 GO:0080111 GO:0090304 GO:0090351 GO:0140097 GO:1901360 GO:1901576 GO:1901652 GO:1901698 GO:1901700 GO:1904975 GO:2000683 GO:2000685
KEGG Pathways
Metabolic & Signaling
ko03030 ko03410 ko03420 ko03430 map03030 map03410 map03420 map03430
Pfam Domains
Protein Families
DNA_ligase_A_C DNA_ligase_A_M DNA_ligase_A_N DRMBL

Protein Analysis

1390

Amino Acids

154.44

Weight (kDa)

6.58

Isoelectric Point (pI)

46.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DRMBL PF07522 262 - 365 2.2e-20 DNA repair metallo-beta-lactamase
DNA_ligase_A_N PF04675 758 - 934 6.1e-41 DNA ligase N terminus
DNA_ligase_A_M PF01068 1004 - 1230 1.5e-46 ATP dependent DNA ligase domain
DNA_ligase_A_C PF04679 1256 - 1363 1.3e-16 ATP dependent DNA ligase C terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015031)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66730
fragaria_vesca FvH4_1g29000 FvH4_1g29000 FvH4_1g29000
malus_domestica MD00G1054100.v1.1
prunus_persica Prupe.3G150300_v2.0.a1
pyrus_communis pycom17g25560
rosa_chinensis RchiOBHm_Chr3g0493691 RchiOBHm_Chr3g0493951
rosa_laevigata RLG00000022694
rosa_roxburghii Rroxscaffold_6G00391370
rosa_rugosa Rorug03G0263400 Rorug03G0263500 Rorug03G0263600
rosa_samantha Rh3AG312500 Rh3AG313200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1592, 2800
AccB1I GGYRCC 4 cut(s) 392, 431, 1889, 3922
AccB7I CCANNNNNTGG 1 cut(s) 4028
AccI GTMKAC 4 cut(s) 147, 1211, 3167, 3360
AccII CGCG 4 cut(s) 297, 609, 762, 1077
AclWI GGATC 6 cut(s) 216, 607, 994, 1836, 1849, 2502
AcoI YGGCCR 4 cut(s) 750, 854, 3097, 3888
AcsI RAATTY 6 cut(s) 563, 1493, 1514, 1873, 2818, 3111
AcuI CTGAAG 5 cut(s) 1910, 2382, 2858, 3071, 3816
AcvI CACGTG 2 cut(s) 3311, 4028
AcyI GRCGYC 1 cut(s) 375
AfaI GTAC 4 cut(s) 2528, 2649, 2913, 3758
AfiI CCNNNNNNNGG 8 cut(s) 286, 425, 1091, 1262, 1263, 1886, 2517, 4028
AflIII ACRYGT 2 cut(s) 1075, 3077
AhlI ACTAGT 1 cut(s) 3121
AjiI CACGTC 1 cut(s) 4147
AjnI CCWGG 5 cut(s) 914, 1421, 1693, 2992, 3996
AjuI GAANNNNNNNTTGG 4 cut(s) 405, 437, 680, 712
Alw21I GWGCWC 4 cut(s) 1463, 2414, 2674, 3109
Alw44I GTGCAC 2 cut(s) 2410, 3105
AlwI GGATC 6 cut(s) 216, 607, 994, 1836, 1849, 2502
AlwNI CAGNNNCTG 4 cut(s) 1273, 1607, 2891, 4103
Ama87I CYCGRG 1 cut(s) 4048
AoxI GGCC 9 cut(s) 244, 634, 750, 854, 866, 1769, 3097, 3888, 3999
ApaLI GTGCAC 2 cut(s) 2410, 3105
ApeKI GCWGC 4 cut(s) 522, 2392, 2426, 2888
ApoI RAATTY 6 cut(s) 563, 1493, 1514, 1873, 2818, 3111
ArsI GACNNNNNNTTYG 6 cut(s) 1745, 1777, 3374, 3406, 3799, 3831
AseI ATTAAT 1 cut(s) 3192
Asp700I GAANNNNTTC 5 cut(s) 1392, 2822, 3560, 3801, 4119
AspLEI GCGC 3 cut(s) 762, 1310, 2632
AspS9I GGNCC 7 cut(s) 22, 244, 289, 496, 1769, 3132, 3712
AsuC2I CCSGG 2 cut(s) 390, 426
AsuII TTCGAA 2 cut(s) 1978, 3483
AvaI CYCGRG 1 cut(s) 4048
AvaII GGWCC 5 cut(s) 22, 289, 496, 3132, 3712
BaeGI GKGCMC 4 cut(s) 436, 1014, 2414, 3109
BaeI ACNNNNGTAYC 4 cut(s) 1287, 1320, 1533, 1566
BalI TGGCCA 3 cut(s) 856, 3099, 3890
BamHI GGATCC 1 cut(s) 1841
BanI GGYRCC 4 cut(s) 392, 431, 1889, 3922
BanII GRGCYC 1 cut(s) 1463
BarI GAAGNNNNNNTAC 6 cut(s) 2455, 2487, 3461, 3493, 4084, 4116
BauI CACGAG 1 cut(s) 248
BbrPI CACGTG 2 cut(s) 3311, 4028
BbsI GAAGAC 3 cut(s) 2147, 3370, 3439
Bbv12I GWGCWC 4 cut(s) 1463, 2414, 2674, 3109
BbvCI CCTCAGC 2 cut(s) 2067, 3918
BbvI GCAGC 4 cut(s) 509, 2379, 2413, 2900
BceAI ACGGC 5 cut(s) 128, 232, 380, 515, 737
BcgI CGANNNNNNTGC 2 cut(s) 3340, 3374
BciT130I CCWGG 5 cut(s) 916, 1423, 1695, 2994, 3998
BciVI GTATCC 3 cut(s) 535, 1567, 3646
BcnI CCSGG 2 cut(s) 390, 426
BcuI ACTAGT 1 cut(s) 3121
BfaI CTAG 3 cut(s) 11, 1445, 3122
BfmI CTRYAG 7 cut(s) 1015, 1212, 2235, 2427, 2850, 2892, 3907
BfoI RGCGCY 2 cut(s) 1311, 2633
BfuAI ACCTGC 2 cut(s) 1592, 2800
BfuI GTATCC 3 cut(s) 535, 1567, 3646
BglII AGATCT 3 cut(s) 2542, 3184, 3882
BisI GCNGC 5 cut(s) 523, 2393, 2427, 2889, 4002
BlsI GCNGC 5 cut(s) 524, 2394, 2428, 2890, 4003
Bme1390I CCNGG 7 cut(s) 390, 426, 916, 1423, 1695, 2994, 3998
Bme18I GGWCC 5 cut(s) 22, 289, 496, 3132, 3712
BmeT110I CYCGRG 1 cut(s) 4048
BmgBI CACGTC 1 cut(s) 4147
BmgT120I GGNCC 7 cut(s) 22, 244, 289, 496, 1769, 3132, 3712
BmrFI CCNGG 7 cut(s) 390, 426, 916, 1423, 1695, 2994, 3998
BmrI ACTGGG 2 cut(s) 225, 2913
BmuI ACTGGG 2 cut(s) 225, 2913
BpiI GAAGAC 3 cut(s) 2147, 3370, 3439
BplI GAGNNNNNCTC 2 cut(s) 2060, 2092
BpmI CTGGAG 4 cut(s) 2272, 2504, 3927, 3972
Bpu10I CCTNAGC 3 cut(s) 1462, 2067, 3918
Bpu14I TTCGAA 2 cut(s) 1978, 3483
BpuEI CTTGAG 6 cut(s) 1016, 1210, 1724, 2327, 2544, 2787
BpuMI CCSGG 2 cut(s) 390, 426
Bsa29I ATCGAT 2 cut(s) 348, 1161
BsaAI YACGTR 4 cut(s) 1054, 2762, 3311, 4028
BsaHI GRCGYC 1 cut(s) 375
BsaI GGTCTC 2 cut(s) 227, 1556
BsaJI CCNNGG 5 cut(s) 915, 1257, 1422, 1845, 3492
BsaXI ACNNNNNCTCC 8 cut(s) 69, 99, 276, 306, 3237, 3267, 3970, 4000
Bsc4I CCNNNNNNNGG 8 cut(s) 286, 425, 1091, 1262, 1263, 1886, 2517, 4028
Bse118I RCCGGY 2 cut(s) 164, 1127
Bse3DI GCAATG 2 cut(s) 1948, 2778
BseBI CCWGG 5 cut(s) 916, 1423, 1695, 2994, 3998
BseCI ATCGAT 2 cut(s) 348, 1161
BseDI CCNNGG 5 cut(s) 915, 1257, 1422, 1845, 3492
BseLI CCNNNNNNNGG 8 cut(s) 286, 425, 1091, 1262, 1263, 1886, 2517, 4028
BseMI GCAATG 2 cut(s) 1948, 2778
BseMII CTCAG 6 cut(s) 1476, 2058, 2091, 3416, 3909, 4142
BseRI GAGGAG 5 cut(s) 269, 902, 1320, 1397, 3442
BseSI GKGCMC 4 cut(s) 436, 1014, 2414, 3109
BseXI GCAGC 4 cut(s) 509, 2379, 2413, 2900
BseYI CCCAGC 1 cut(s) 1151
BsgI GTGCAG 2 cut(s) 3298, 3990
Bsh1236I CGCG 4 cut(s) 297, 609, 762, 1077
BshFI GGCC 9 cut(s) 246, 636, 752, 856, 868, 1771, 3099, 3890, 4001
BshNI GGYRCC 4 cut(s) 392, 431, 1889, 3922
BshVI ATCGAT 2 cut(s) 348, 1161
BsiHKAI GWGCWC 4 cut(s) 1463, 2414, 2674, 3109
BsiHKCI CYCGRG 1 cut(s) 4048
BsiSI CCGG 5 cut(s) 165, 389, 426, 865, 1128
BslFI GGGAC 6 cut(s) 401, 917, 2512, 3173, 3698, 3890
BslI CCNNNNNNNGG 8 cut(s) 286, 425, 1091, 1262, 1263, 1886, 2517, 4028
BsmBI CGTCTC 3 cut(s) 184, 281, 304
BsmFI GGGAC 6 cut(s) 401, 917, 2512, 3173, 3698, 3890
BsnI GGCC 9 cut(s) 246, 636, 752, 856, 868, 1771, 3099, 3890, 4001
Bso31I GGTCTC 2 cut(s) 227, 1556
BsoBI CYCGRG 1 cut(s) 4048
Bsp119I TTCGAA 2 cut(s) 1978, 3483
Bsp1286I GDGCHC 6 cut(s) 436, 1014, 1463, 2414, 2674, 3109
Bsp1407I TGTACA 2 cut(s) 2526, 2647
Bsp19I CCATGG 2 cut(s) 1257, 1845
BspANI GGCC 9 cut(s) 246, 636, 752, 856, 868, 1771, 3099, 3890, 4001
BspCNI CTCAG 6 cut(s) 1475, 2059, 2090, 3417, 3910, 4141
BspDI ATCGAT 2 cut(s) 348, 1161
BspFNI CGCG 4 cut(s) 297, 609, 762, 1077
BspHI TCATGA 1 cut(s) 2946
BspMAI CTGCAG 1 cut(s) 2431
BspMI ACCTGC 2 cut(s) 1592, 2800
BspPI GGATC 6 cut(s) 216, 607, 994, 1836, 1849, 2502
BspT104I TTCGAA 2 cut(s) 1978, 3483
BspT107I GGYRCC 4 cut(s) 392, 431, 1889, 3922
BspTNI GGTCTC 2 cut(s) 227, 1556
BsrDI GCAATG 2 cut(s) 1948, 2778
BsrFI RCCGGY 2 cut(s) 164, 1127
BsrGI TGTACA 2 cut(s) 2526, 2647
BssAI RCCGGY 2 cut(s) 164, 1127
BssECI CCNNGG 5 cut(s) 915, 1257, 1422, 1845, 3492
BssNI GRCGYC 1 cut(s) 375
BssSI CACGAG 1 cut(s) 248
BssT1I CCWWGG 3 cut(s) 1257, 1845, 3492
Bst2BI CACGAG 1 cut(s) 248
Bst2UI CCWGG 5 cut(s) 916, 1423, 1695, 2994, 3998
Bst6I CTCTTC 3 cut(s) 307, 659, 2974
BstACI GRCGYC 1 cut(s) 375
BstAPI GCANNNNNTGC 1 cut(s) 3518
BstAUI TGTACA 2 cut(s) 2526, 2647
BstBAI YACGTR 4 cut(s) 1054, 2762, 3311, 4028
BstBI TTCGAA 2 cut(s) 1978, 3483
BstDEI CTNAG 8 cut(s) 240, 1043, 1462, 2067, 2077, 3425, 3918, 4128
BstDSI CCRYGG 2 cut(s) 1257, 1845
BstENI CCTNNNNNAGG 1 cut(s) 284
BstFNI CGCG 4 cut(s) 297, 609, 762, 1077
BstH2I RGCGCY 2 cut(s) 1311, 2633
BstHHI GCGC 3 cut(s) 762, 1310, 2632
BstMWI GCNNNNNNNGC 8 cut(s) 149, 165, 1467, 3518, 3527, 3536, 3545, 3643
BstNI CCWGG 5 cut(s) 916, 1423, 1695, 2994, 3998
BstSCI CCNGG 7 cut(s) 388, 424, 914, 1421, 1693, 2992, 3996
BstSFI CTRYAG 7 cut(s) 1015, 1212, 2235, 2427, 2850, 2892, 3907
BstSLI GKGCMC 4 cut(s) 436, 1014, 2414, 3109
BstSNI TACGTA 1 cut(s) 2762
BstUI CGCG 4 cut(s) 297, 609, 762, 1077
BstV1I GCAGC 4 cut(s) 509, 2379, 2413, 2900
BstV2I GAAGAC 3 cut(s) 2147, 3370, 3439
BstX2I RGATCY 6 cut(s) 999, 1841, 2507, 2542, 3184, 3882
BstYI RGATCY 6 cut(s) 999, 1841, 2507, 2542, 3184, 3882
Bsu15I ATCGAT 2 cut(s) 348, 1161
BsuI GTATCC 3 cut(s) 535, 1567, 3646
BsuRI GGCC 9 cut(s) 246, 636, 752, 856, 868, 1771, 3099, 3890, 4001
BsuTUI ATCGAT 2 cut(s) 348, 1161
BtgI CCRYGG 2 cut(s) 1257, 1845
BtrI CACGTC 1 cut(s) 4147
BtsI GCAGTG 3 cut(s) 1581, 2013, 2674
BveI ACCTGC 2 cut(s) 1592, 2800
CaiI CAGNNNCTG 4 cut(s) 1273, 1607, 2891, 4103
CciI TCATGA 1 cut(s) 2946
CfoI GCGC 3 cut(s) 762, 1310, 2632
Cfr10I RCCGGY 2 cut(s) 164, 1127
Cfr13I GGNCC 7 cut(s) 22, 244, 289, 496, 1769, 3132, 3712
ClaI ATCGAT 2 cut(s) 348, 1161
CseI GACGC 6 cut(s) 158, 286, 383, 751, 2867, 3799
Csp6I GTAC 4 cut(s) 2527, 2648, 2912, 3757
CviQI GTAC 4 cut(s) 2527, 2648, 2912, 3757
DdeI CTNAG 8 cut(s) 240, 1043, 1462, 2067, 2077, 3425, 3918, 4128
EaeI YGGCCR 4 cut(s) 750, 854, 3097, 3888
Eam1104I CTCTTC 3 cut(s) 307, 659, 2974
EarI CTCTTC 3 cut(s) 307, 659, 2974
Ecl136II GAGCTC 1 cut(s) 1461
Eco105I TACGTA 1 cut(s) 2762
Eco130I CCWWGG 3 cut(s) 1257, 1845, 3492
Eco24I GRGCYC 1 cut(s) 1463
Eco31I GGTCTC 2 cut(s) 227, 1556
Eco47I GGWCC 5 cut(s) 22, 289, 496, 3132, 3712
Eco53kI GAGCTC 1 cut(s) 1461
Eco57I CTGAAG 5 cut(s) 1910, 2382, 2858, 3071, 3816
Eco72I CACGTG 2 cut(s) 3311, 4028
Eco88I CYCGRG 1 cut(s) 4048
EcoICRI GAGCTC 1 cut(s) 1461
EcoNI CCTNNNNNAGG 1 cut(s) 284
EcoO109I RGGNCCY 2 cut(s) 244, 289
EcoRI GAATTC 1 cut(s) 2818
EcoRII CCWGG 5 cut(s) 914, 1421, 1693, 2992, 3996
EcoT14I CCWWGG 3 cut(s) 1257, 1845, 3492
EcoT22I ATGCAT 5 cut(s) 2248, 2494, 3226, 3334, 3577
EcoT38I GRGCYC 1 cut(s) 1463
ErhI CCWWGG 3 cut(s) 1257, 1845, 3492
Esp3I CGTCTC 3 cut(s) 184, 281, 304
FalI AAGNNNNNCTT 2 cut(s) 405, 437
FaqI GGGAC 6 cut(s) 401, 917, 2512, 3173, 3698, 3890
FauI CCCGC 2 cut(s) 72, 2489
FblI GTMKAC 4 cut(s) 147, 1211, 3167, 3360
Fnu4HI GCNGC 5 cut(s) 523, 2393, 2427, 2889, 4002
FriOI GRGCYC 1 cut(s) 1463
Fsp4HI GCNGC 5 cut(s) 523, 2393, 2427, 2889, 4002
FspBI CTAG 3 cut(s) 11, 1445, 3122
GlaI GCGC 3 cut(s) 761, 1309, 2631
GluI GCNGC 5 cut(s) 523, 2393, 2427, 2889, 4002
GsaI CCCAGC 1 cut(s) 1155
GsuI CTGGAG 4 cut(s) 2272, 2504, 3927, 3972
HaeII RGCGCY 2 cut(s) 1311, 2633
HaeIII GGCC 9 cut(s) 246, 636, 752, 856, 868, 1771, 3099, 3890, 4001
HapII CCGG 5 cut(s) 165, 389, 426, 865, 1128
HgaI GACGC 6 cut(s) 158, 286, 383, 751, 2867, 3799
HhaI GCGC 3 cut(s) 762, 1310, 2632
Hin1I GRCGYC 1 cut(s) 375
Hin6I GCGC 3 cut(s) 760, 1308, 2630
HinP1I GCGC 3 cut(s) 760, 1308, 2630
HincII GTYRAC 6 cut(s) 148, 373, 1234, 1368, 3168, 3361
HindII GTYRAC 6 cut(s) 148, 373, 1234, 1368, 3168, 3361
HindIII AAGCTT 3 cut(s) 2870, 3069, 3415
HpaI GTTAAC 1 cut(s) 1368
HpaII CCGG 5 cut(s) 165, 389, 426, 865, 1128
Hpy99I CGWCG 2 cut(s) 149, 152
HpyCH4IV ACGT 7 cut(s) 837, 1053, 1626, 2761, 3310, 4027, 4146
HpyF10VI GCNNNNNNNGC 8 cut(s) 149, 165, 1467, 3518, 3527, 3536, 3545, 3643
HpyF3I CTNAG 8 cut(s) 240, 1043, 1462, 2067, 2077, 3425, 3918, 4128
HpySE526I ACGT 7 cut(s) 837, 1053, 1626, 2761, 3310, 4027, 4146
Hsp92I GRCGYC 1 cut(s) 375
HspAI GCGC 3 cut(s) 760, 1308, 2630
KroI GCCGGC 1 cut(s) 164
KroNI GCCGGC 1 cut(s) 166
KspAI GTTAAC 1 cut(s) 1368
LmnI GCTCC 8 cut(s) 263, 1315, 1458, 2275, 2446, 3321, 3722, 4164
Lsp1109I GCAGC 4 cut(s) 509, 2379, 2413, 2900
MaeI CTAG 3 cut(s) 11, 1445, 3122
MaeII ACGT 7 cut(s) 837, 1053, 1626, 2761, 3310, 4027, 4146
MfeI CAATTG 2 cut(s) 1319, 3717
MflI RGATCY 6 cut(s) 999, 1841, 2507, 2542, 3184, 3882
MhlI GDGCHC 6 cut(s) 436, 1014, 1463, 2414, 2674, 3109
MlsI TGGCCA 3 cut(s) 856, 3099, 3890
MluI ACGCGT 1 cut(s) 1075
MluNI TGGCCA 3 cut(s) 856, 3099, 3890
MlyI GAGTC 2 cut(s) 1769, 3173
MmeI TCCRAC 6 cut(s) 355, 474, 678, 1737, 2112, 3235
Mox20I TGGCCA 3 cut(s) 856, 3099, 3890
Mph1103I ATGCAT 5 cut(s) 2248, 2494, 3226, 3334, 3577
MreI CGCCGGCG 1 cut(s) 164
MroNI GCCGGC 1 cut(s) 164
MroXI GAANNNNTTC 5 cut(s) 1392, 2822, 3560, 3801, 4119
MscI TGGCCA 3 cut(s) 856, 3099, 3890
MseI TTAA 7 cut(s) 1367, 1677, 1952, 3192, 3227, 3413, 3662
MslI CAYNNNNRTG 3 cut(s) 462, 735, 2439
Msp20I TGGCCA 3 cut(s) 856, 3099, 3890
MspA1I CMGCKG 3 cut(s) 2114, 2891, 3917
MspI CCGG 5 cut(s) 165, 389, 426, 865, 1128
MspR9I CCNGG 7 cut(s) 390, 426, 916, 1423, 1695, 2994, 3998
MunI CAATTG 2 cut(s) 1319, 3717
MvaI CCWGG 5 cut(s) 916, 1423, 1695, 2994, 3998
MvnI CGCG 4 cut(s) 297, 609, 762, 1077
MwoI GCNNNNNNNGC 8 cut(s) 149, 165, 1467, 3518, 3527, 3536, 3545, 3643
NaeI GCCGGC 1 cut(s) 166
NciI CCSGG 2 cut(s) 390, 426
NcoI CCATGG 2 cut(s) 1257, 1845
NgoMIV GCCGGC 1 cut(s) 164
NmeAIII GCCGAG 1 cut(s) 1664
NmuCI GTSAC 4 cut(s) 845, 1426, 1577, 4066
NsiI ATGCAT 5 cut(s) 2248, 2494, 3226, 3334, 3577
NspV TTCGAA 2 cut(s) 1978, 3483
PaeI GCATGC 4 cut(s) 2246, 2250, 2496, 2568
PagI TCATGA 1 cut(s) 2946
PciI ACATGT 1 cut(s) 3077
PcsI WCGNNNNNNNCGW 3 cut(s) 354, 2758, 3366
PdiI GCCGGC 1 cut(s) 166
PdmI GAANNNNTTC 5 cut(s) 1392, 2822, 3560, 3801, 4119
PflMI CCANNNNNTGG 1 cut(s) 4028
PfoI TCCNGGA 1 cut(s) 2992
PkrI GCNGC 5 cut(s) 524, 2394, 2428, 2890, 4003
PleI GAGTC 2 cut(s) 1768, 3172
PmaCI CACGTG 2 cut(s) 3311, 4028
PmlI CACGTG 2 cut(s) 3311, 4028
PpsI GAGTC 2 cut(s) 1768, 3172
Ppu21I YACGTR 4 cut(s) 1054, 2762, 3311, 4028
PpuMI RGGWCCY 1 cut(s) 289
PscI ACATGT 1 cut(s) 3077
PshBI ATTAAT 1 cut(s) 3192
Psp124BI GAGCTC 1 cut(s) 1463
Psp5II RGGWCCY 1 cut(s) 289
Psp6I CCWGG 5 cut(s) 914, 1421, 1693, 2992, 3996
PspCI CACGTG 2 cut(s) 3311, 4028
PspFI CCCAGC 1 cut(s) 1151
PspGI CCWGG 5 cut(s) 914, 1421, 1693, 2992, 3996
PspPI GGNCC 7 cut(s) 22, 244, 289, 496, 1769, 3132, 3712
PspPPI RGGWCCY 1 cut(s) 289
PstI CTGCAG 1 cut(s) 2431
PstNI CAGNNNCTG 4 cut(s) 1273, 1607, 2891, 4103
PsuI RGATCY 6 cut(s) 999, 1841, 2507, 2542, 3184, 3882
PvuII CAGCTG 2 cut(s) 2891, 3917
RsaI GTAC 4 cut(s) 2528, 2649, 2913, 3758
RsaNI GTAC 4 cut(s) 2527, 2648, 2912, 3757
RseI CAYNNNNRTG 3 cut(s) 462, 735, 2439
SacI GAGCTC 1 cut(s) 1463
SalI GTCGAC 3 cut(s) 146, 3166, 3359
SaqAI TTAA 7 cut(s) 1367, 1677, 1952, 3192, 3227, 3413, 3662
SatI GCNGC 5 cut(s) 523, 2393, 2427, 2889, 4002
Sau96I GGNCC 7 cut(s) 22, 244, 289, 496, 1769, 3132, 3712
SchI GAGTC 2 cut(s) 1769, 3173
ScrFI CCNGG 7 cut(s) 390, 426, 916, 1423, 1695, 2994, 3998
SduI GDGCHC 6 cut(s) 436, 1014, 1463, 2414, 2674, 3109
SfcI CTRYAG 7 cut(s) 1015, 1212, 2235, 2427, 2850, 2892, 3907
SfuI TTCGAA 2 cut(s) 1978, 3483
SgrAI CRCCGGYG 1 cut(s) 164
SgrDI CGTCGACG 1 cut(s) 146
SinI GGWCC 5 cut(s) 22, 289, 496, 3132, 3712
SmiMI CAYNNNNRTG 3 cut(s) 462, 735, 2439
SmlI CTYRAG 6 cut(s) 995, 1189, 1739, 2306, 2559, 2802
SmoI CTYRAG 6 cut(s) 995, 1189, 1739, 2306, 2559, 2802
SnaBI TACGTA 1 cut(s) 2762
SpeI ACTAGT 1 cut(s) 3121
SphI GCATGC 4 cut(s) 2246, 2250, 2496, 2568
SspI AATATT 1 cut(s) 1621
SspMI CTAG 3 cut(s) 11, 1445, 3122
SstI GAGCTC 1 cut(s) 1463
StyD4I CCNGG 7 cut(s) 388, 424, 914, 1421, 1693, 2992, 3996
StyI CCWWGG 3 cut(s) 1257, 1845, 3492
TaiI ACGT 7 cut(s) 840, 1056, 1629, 2764, 3313, 4030, 4149
TatI WGTACW 3 cut(s) 2526, 2647, 2911
TauI GCSGC 1 cut(s) 4004
Tru1I TTAA 7 cut(s) 1367, 1677, 1952, 3192, 3227, 3413, 3662
Tru9I TTAA 7 cut(s) 1367, 1677, 1952, 3192, 3227, 3413, 3662
TseFI GTSAC 4 cut(s) 845, 1426, 1577, 4066
TseI GCWGC 4 cut(s) 522, 2392, 2426, 2888
Tsp45I GTSAC 4 cut(s) 845, 1426, 1577, 4066
TspGWI ACGGA 5 cut(s) 35, 166, 635, 809, 3124
Van91I CCANNNNNTGG 1 cut(s) 4028
VneI GTGCAC 2 cut(s) 2410, 3105
VpaK11BI GGWCC 5 cut(s) 22, 289, 496, 3132, 3712
VspI ATTAAT 1 cut(s) 3192
XagI CCTNNNNNAGG 1 cut(s) 284
XapI RAATTY 6 cut(s) 563, 1493, 1514, 1873, 2818, 3111
XbaI TCTAGA 1 cut(s) 10
XcmI CCANNNNNNNNNTGG 4 cut(s) 2095, 2926, 3723, 3951
XmiI GTMKAC 4 cut(s) 147, 1211, 3167, 3360
XmnI GAANNNNTTC 5 cut(s) 1392, 2822, 3560, 3801, 4119
XspI CTAG 3 cut(s) 11, 1445, 3122
Zsp2I ATGCAT 5 cut(s) 2248, 2494, 3226, 3334, 3577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.