Rroxscaffold_6G00391370

DNA ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0015031
Physical Location & Seq
Forward (+)
9305055 .. 9317084
12030 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00391370.1

Sequence Viewer

Length: 4200 bp
ATGACGGCTCTGGATTCTACGGACCTCTTCCTGACTGCCCTAACCGCCTTTTCCCACCCACCCAGACCCCGACCCCTCGAACTCTCTCTCCCTCCGATTCCCTCCACATTCCCCCACTCCAAACTCATCCCCAGAACCCGTTTCGCCGTCGACGCATTCCGCTATGCCGGCGATTATTCCGTCGCTTACTTCCTCTCTCACTTCCACTCCGATCACTACGGCGGTCTCTCCCCCAATTGGTCCCAAGGCCTCGTCTTTTGCTCCCCCACCACCGCTCGTCTTCTCACAGACGTCCTCAACGTCTCTTCGCTTTTCGTGGTTCCTCTGCCGCTCGGACAGCCCCTTCTCATTGACGGATGCGAGGTCGTGCTTATTGACGCCAATCATTGCCCAGGCGCCGTTCAGTTCTTGTTCAAAGTTCCCGTTCCCGCCTCCAATGGAGACTTCCAGAGGTATGTTCACACTGGTGATTTTCGGTTTTCTGCTTCCATGAAGCTAGACCCTTTTCTCTGTAAATTTGTTGGTTCAGACGCCGTTTTCTTGGATACAACTTATTGCAATCCCAAATTCGTGTTTCCTTCACAAGAAGAATCTGTTGATTATATTTCTAGCTTGATAGAAAGGGTTGGAGGTGAACGTGAAGTTTCTATGAAAAGTGTCCTGTTCCTTGTTGCTACATATGTGATTGGGAAAGAGAAGATTTTGATGGAAATTGCTCGTAGATGTAAGCGTAAAGTGTATGTGGATGCTCGAAAAATGGCGGTGCTGCGTGTTTTGGGTTACGGAGACAGTGGGGTGTTTACAGAGGATGAATGTGAGAGTGATGTGCATGTTGTTGGGTGGAATGTGTTGGGTGAGACTTGGCCATATTTCAGGCCAAATTTTGTGAAAATGGAGGAGATTATGGTTGACAGGGGGTATTCCAGGGTTGTAGGGTTTGTCCCTACTGGCTGGACTTATGAAGTGAAGCGTAACAAGTTCTCGGTGAGGTCAAAGGATGCTTTTGAGATCCATCTTGTACCCTACAGTGAACATTCAAGCTATGATGAACTTAGAGAATACGTCAGGTTTTTGAAACCAAAGCGTGTGATTCCTACAGTGGGCTCCGATGTGGAAAAGATAGACAGTAAACAGGCCAGTAAAATGCAAAAGCATTTTGCTGGGTTAATTGATGAAATGGCCAACAAAAAAGAGTTCTTAAGGGGTTTTCATCGTGGGACTACAGAAGGAGGTGGGAAGGTTGACAGTGATGCCAAAGATTACACGATGGAGGGGCAGGATCAAGAGAAAAAGGCAACACCTTCAGATGTTGATACTATGGGAGATACTAATGTAGGCTTTGGTAAGGAATTGTTGTCTGCTCTCCAAGAACCTGATTCACAAACTCCAATATTGTTAACTGATGAAAAAGCAGAGGAAATTATTCAAGAACTTTGTGATTGTTTACCGTCATGGGTCACTCGCCAGCAGATGCTAGATCTGATTGGGAGCTCAGGAGGGGACATTGTTGAAGCAGTCACTAAATTTTACGATCGTGAAACAGAATTTCATGATCAAGGAATTGCCTCTACAAGTGCTGTATCTGTATCCGAGACCAGCTCACTCTGTGACACAGCATCACCGACAAAAGCAGGTTCTGTTCATGCAAATATTGATGTTTCTTCAAGCCAAGCTCATATTTCACCCAACCCAAGAAATATTTTTAAAAGTGGCATTTTCCCTGGAAAAAGAGGGAGGAAGATCAGTAATAAAGTTAACAAGAAAGTAAAGTTACAGTCAAAATTGAATTCGTGTGGGCCAAAACAATCGACAATAACAAGGTTTTTTAGTAAAGTTTTGCCTGACGTTACAGAAAGTGCAGAGATTGGATCAGTAGGTGAACAAAACCCTAAAGATAAAAAAGTACCAGATCATGTCACCCAATCATACAAGGATGCAATAGATCAATTTCTTCAGATAATAGATGGCAATGAATCATTAAAAAGCTATGCTGGTACCATTCTTCAAAAGGCAAATGGGGATATTAGTATGGCTGTGGATATACATTACCGTAATAATGAAGGCAGGTCTGATGAGAATGAAGTGGAGCTGGTGGTTGATGCAAAATCAGTTCAGTCCAACTGTGGTATAGATAACTGTTCTTCTGTTAAAAAAAAAATTGAATTAGGGAAAATAGGAGTGGTAGCTGATTTGTCTGTACAGGGGTCATTACCAGATAACGTTGCTGCAACTTCTGTGTCACTACCACCAGAAAAATACAATCCTGTAGATCATGCATGCTGGAGCAATGGACAACATGCTCCATATTTACATCTTGCACGAACCTTTGACTTACTAGAGGATGAAAAAGGCAAGATAAAGGCTACTTCTATGCTCTGCAATATGTTCAGAAGTCTGCTTGCCCTGTCTCCTGATGATGTGCTGCCTTCAGTTTATTTGTGCACCAATAAGATAGCAGCAGACCACGAAAATGTGGAACTGAACATTGGTGGAAGTTTGGTCACAGCAGCACTGGAGGACGCATGTGGGACAAGCAGATCCAAAATCAGGGATATGTACAATGAATTGGGAGATCTTGGTGATGTTGCTCAAGCATGCCGGCAGACACAGACTTTGCTTGCCCCTCCTCCACCACTTCTAATAAAAGATGTATTTTCTGCACTCCGGAAGATAAGTGTACAAACAGGTACTGGAAGCAGTGCTCGAAAGAGAAGCCTGATTGTGAATCTCTTGCGTTCTTGCAGAGAGAAAGAGATGAAGTTCCTTGTTAGAACTTTGGTTCGAAACTTACGTATCGGAGCAATGATGAAAACTGTCCTACCTGCTCTGGCTCAAGCTGTTGTTCTGAATTCCTTCCATAGGTGTAATCACGAAGGAACTATAGAAAGTTTGAAGGACAAGCTTCAGCGCCATTCAGCAGCTGTAGTAGACGCATACAATGTACTGCCCAATTTGGATTTGGTCATTCCTTCTCTCATGAAAAGAGGCATTGGATTCTCCTCATCAACTTTGTCAATGGTTCCAGGAACACCTATCAAGCCAATGCTTGCAAGAATCACAAATGGTGTTCAGCAAGCACTGAAGCTATTTGAAAATAAAGCTTTTACATGTGAATACAAATATGATGGCCAGCGTGCACAAATTCATAAACTAGTTGATGGGTCCATGCATGTTTTTACAAGAAATGGGGATGAGTCAACTTCGAGATTTCCAGATCTGATTAATATAATCAATCAATCTTGTAAACCCGATGCAGTGACTTTCATACTGGATGCAGAGGTTGTTGGAGTTGATAGGAAGAATGGTTGCAAGCTAATGTCCTTCCAAGAATTATCTTCACGTGGGAGAGGGAGCAGAGATGCATCAATAACGTTGGATAGCATAAAGGTCAACATTTGCGTCTTTGTCTTCGACATCATGTTTGCCAATGGACAACAGTTGTTAAGCTTTCCTCTGCGGAGAAGGCGAAAGTATTTGAAAGAGTTGTTCTATGATGAGAAGTTGGGTTACTTTGAATATGCCAAGGAAATGACAGTTGAGGCAGAGGATGCTTGTTTAACCAGTGAAGCGACATTAACCAAAATAAACTCTTTCCTTGAAAATGCATTTGTTTCCTCATGTGAAGGGATTATAGTTAAATCTTTAGATGTTGATGCTGGATATTCTCCATCAAAGCGTACAGATACTTGGTTAAAGGTCAAGCGAGACTACATGGAGGGATTGGGTGATACTCTTGATTTAGTCCCAATTGGAGCTTGGCATGGAAACGGGAGAAAGGCAGGCTGGTACAGTCCATTTCTCATGGCATGTTACAATCCTGAAACTGAAGAATTTCAAAGCGTCTGTCGTGTTATGTCTGGGTTTTCAGATTTATTTTATACAGAGATGAAAAGTTTTTATTCTGGGGACAAGATCTTGGCCAAAAAACCACCTTATTATAGAACAGCTGAGGTGCCTGATATGTGGTTTTCTCCTGAGCTTGTATGGGAAATAAGAGGTGCAGACTTTACAGTGTCTCCAGTTCACCAGGCTGCTTTAGGTCTAGTTCATCCATCACGTGGCATTTCCATTCGATTCCCAAGATACATTCGCACTGTGACTGATAGAAAACCAGATGAATGTAGCACAGCTTCTGATTTAGCTGAAATGTTTCATTCTCAGACGAGGAAGATGGATGTGGCTTCTGAAGATGGCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000012 GO:0000726 GO:0002237 GO:0003006 GO:0003674 GO:0003824 GO:0003909 GO:0003910 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006266 GO:0006271 GO:0006273 GO:0006281 GO:0006302 GO:0006303 GO:0006304 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009314 GO:0009409 GO:0009411 GO:0009416 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009791 GO:0009845 GO:0009987 GO:0010033 GO:0010154 GO:0010225 GO:0010243 GO:0015074 GO:0016070 GO:0016874 GO:0016886 GO:0022414 GO:0022616 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0033554 GO:0034641 GO:0034645 GO:0035510 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044728 GO:0046483 GO:0046677 GO:0048316 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051103 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0065007 GO:0070013 GO:0070988 GO:0071704 GO:0080111 GO:0090304 GO:0090351 GO:0140097 GO:1901360 GO:1901576 GO:1901652 GO:1901698 GO:1901700 GO:1904975 GO:2000683 GO:2000685
KEGG Pathways
Metabolic & Signaling
ko03030 ko03410 ko03420 ko03430 map03030 map03410 map03420 map03430
Pfam Domains
Protein Families
DNA_ligase_A_C DNA_ligase_A_M DNA_ligase_A_N DRMBL

Protein Analysis

1399

Amino Acids

155.48

Weight (kDa)

6.36

Isoelectric Point (pI)

43.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DRMBL PF07522 265 - 368 4.5e-21 DNA repair metallo-beta-lactamase
DNA_ligase_A_N PF04675 768 - 944 6.8e-43 DNA ligase N terminus
DNA_ligase_A_M PF01068 1014 - 1234 1.3e-46 ATP dependent DNA ligase domain
DNA_ligase_A_C PF04679 1260 - 1367 1.7e-16 ATP dependent DNA ligase C terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015031)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66730
fragaria_vesca FvH4_1g29000 FvH4_1g29000 FvH4_1g29000
malus_domestica MD00G1054100.v1.1
prunus_persica Prupe.3G150300_v2.0.a1
pyrus_communis pycom17g25560
rosa_chinensis RchiOBHm_Chr3g0493691 RchiOBHm_Chr3g0493951
rosa_laevigata RLG00000022694
rosa_roxburghii Rroxscaffold_6G00391370
rosa_rugosa Rorug03G0263400 Rorug03G0263500 Rorug03G0263600
rosa_samantha Rh3AG312500 Rh3AG313200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 294
Acc36I ACCTGC 3 cut(s) 1622, 2055, 2830
Acc65I GGTACC 1 cut(s) 1994
AccB1I GGYRCC 3 cut(s) 395, 1994, 3952
AccB7I CCANNNNNTGG 1 cut(s) 4058
AccBSI CCGCTC 2 cut(s) 275, 331
AccI GTMKAC 2 cut(s) 150, 2928
AccIII TCCGGA 1 cut(s) 2664
AciI CCGC 8 cut(s) 45, 160, 222, 273, 329, 429, 761, 3457
AclI AACGTT 2 cut(s) 2220, 3371
AclWI GGATC 4 cut(s) 1003, 1287, 1876, 2532
AcoI YGGCCR 4 cut(s) 863, 1179, 3127, 3918
AcsI RAATTY 9 cut(s) 515, 566, 880, 1523, 1544, 1786, 2848, 3141, 3830
AcuI CTGAAG 6 cut(s) 1287, 1937, 2412, 2888, 3101, 3846
AcvI CACGTG 2 cut(s) 3341, 4058
AcyI GRCGYC 4 cut(s) 291, 378, 396, 531
AdeI CACNNNGTG 1 cut(s) 1607
AfiI CCNNNNNNNGG 5 cut(s) 237, 1100, 2547, 2859, 4058
AflII CTTAAG 1 cut(s) 1198
AflIII ACRYGT 1 cut(s) 3107
AhlI ACTAGT 1 cut(s) 3151
AjnI CCWGG 5 cut(s) 391, 923, 1720, 3022, 4026
AjuI GAANNNNNNNTTGG 4 cut(s) 428, 460, 2469, 2501
AleI CACNNNNGTG 1 cut(s) 465
AloI GAACNNNNNNTCC 2 cut(s) 72, 104
Alw21I GWGCWC 4 cut(s) 1493, 2444, 2704, 3139
Alw26I GTCTC 9 cut(s) 230, 307, 435, 780, 851, 1586, 2412, 3699, 4020
Alw44I GTGCAC 2 cut(s) 2440, 3135
AlwI GGATC 4 cut(s) 1003, 1287, 1876, 2532
AlwNI CAGNNNCTG 4 cut(s) 1637, 2690, 2921, 4133
Aor13HI TCCGGA 1 cut(s) 2664
AoxI GGCC 8 cut(s) 247, 863, 875, 1134, 1179, 1796, 3127, 3918
ApaLI GTGCAC 2 cut(s) 2440, 3135
ApeKI GCWGC 8 cut(s) 766, 2225, 2422, 2456, 2507, 2918, 4031, 4194
ApoI RAATTY 9 cut(s) 515, 566, 880, 1523, 1544, 1786, 2848, 3141, 3830
ArsI GACNNNNNNTTYG 6 cut(s) 2596, 2628, 3404, 3436, 3829, 3861
AseI ATTAAT 1 cut(s) 3222
Asp700I GAANNNNTTC 4 cut(s) 1422, 2852, 3831, 4149
Asp718I GGTACC 1 cut(s) 1994
AspLEI GCGC 2 cut(s) 398, 2910
AspS9I GGNCC 4 cut(s) 22, 240, 1796, 3162
AsuII TTCGAA 1 cut(s) 2782
AvaII GGWCC 3 cut(s) 22, 240, 3162
BaeGI GKGCMC 2 cut(s) 2444, 3139
BaeI ACNNNNGTAYC 4 cut(s) 1317, 1350, 2776, 2809
BalI TGGCCA 4 cut(s) 865, 1181, 3129, 3920
BanI GGYRCC 3 cut(s) 395, 1994, 3952
BanII GRGCYC 2 cut(s) 1106, 1493
BarI GAAGNNNNNNTAC 4 cut(s) 3491, 3523, 4114, 4146
BbrPI CACGTG 2 cut(s) 3341, 4058
BbsI GAAGAC 2 cut(s) 272, 3400
Bbv12I GWGCWC 4 cut(s) 1493, 2444, 2704, 3139
BbvCI CCTCAGC 1 cut(s) 3948
BbvI GCAGC 7 cut(s) 753, 2212, 2409, 2468, 2519, 2930, 4018
BceAI ACGGC 5 cut(s) 21, 131, 235, 383, 518
BciT130I CCWGG 5 cut(s) 393, 925, 1722, 3024, 4028
BciVI GTATCC 2 cut(s) 538, 1597
BclI TGATCA 1 cut(s) 1552
BcoDI GTCTC 9 cut(s) 230, 307, 435, 780, 851, 1586, 2412, 3699, 4020
BcuI ACTAGT 1 cut(s) 3151
BfaI CTAG 6 cut(s) 497, 609, 1475, 2336, 3152, 4043
BfmI CTRYAG 6 cut(s) 1024, 1095, 1221, 2265, 2880, 2922
BfoI RGCGCY 2 cut(s) 399, 2911
BfrI CTTAAG 1 cut(s) 1198
BfuAI ACCTGC 3 cut(s) 1622, 2055, 2830
BfuI GTATCC 2 cut(s) 538, 1597
BglII AGATCT 4 cut(s) 1477, 2572, 3214, 3912
BisI GCNGC 9 cut(s) 329, 767, 2226, 2423, 2457, 2508, 2919, 4032, 4195
BlsI GCNGC 9 cut(s) 330, 768, 2227, 2424, 2458, 2509, 2920, 4033, 4196
Bme1390I CCNGG 5 cut(s) 393, 925, 1722, 3024, 4028
Bme18I GGWCC 3 cut(s) 22, 240, 3162
BmgT120I GGNCC 4 cut(s) 22, 240, 1796, 3162
BmiI GGNNCC 8 cut(s) 242, 321, 397, 1105, 1996, 3021, 3163, 3954
BmrFI CCNGG 5 cut(s) 393, 925, 1722, 3024, 4028
BpiI GAAGAC 2 cut(s) 272, 3400
BplI GAGNNNNNCTC 2 cut(s) 1583, 1615
BpmI CTGGAG 3 cut(s) 2302, 2534, 4002
Bpu10I CCTNAGC 3 cut(s) 1492, 3948, 3975
Bpu14I TTCGAA 1 cut(s) 2782
BpuEI CTTGAG 2 cut(s) 2574, 2817
BsaAI YACGTR 3 cut(s) 2792, 3341, 4058
BsaHI GRCGYC 4 cut(s) 291, 378, 396, 531
BsaI GGTCTC 2 cut(s) 230, 1586
BsaJI CCNNGG 5 cut(s) 244, 391, 924, 1720, 3522
BsaWI WCCGGW 1 cut(s) 2664
BsaXI ACNNNNNCTCC 6 cut(s) 72, 102, 191, 221, 4000, 4030
Bsc4I CCNNNNNNNGG 5 cut(s) 237, 1100, 2547, 2859, 4058
Bse118I RCCGGY 2 cut(s) 167, 2598
Bse1I ACTGG 8 cut(s) 469, 952, 1137, 2517, 2695, 3273, 3561, 4019
Bse3DI GCAATG 4 cut(s) 385, 1975, 2293, 2808
BseAI TCCGGA 1 cut(s) 2664
BseBI CCWGG 5 cut(s) 393, 925, 1722, 3024, 4028
BseDI CCNNGG 5 cut(s) 244, 391, 924, 1720, 3522
BseLI CCNNNNNNNGG 5 cut(s) 237, 1100, 2547, 2859, 4058
BseMI GCAATG 4 cut(s) 385, 1975, 2293, 2808
BseMII CTCAG 4 cut(s) 1506, 3939, 3966, 4172
BseNI ACTGG 8 cut(s) 469, 952, 1137, 2517, 2695, 3273, 3561, 4019
BseRI GAGGAG 3 cut(s) 911, 2616, 2989
BseSI GKGCMC 2 cut(s) 2444, 3139
BseXI GCAGC 7 cut(s) 753, 2212, 2409, 2468, 2519, 2930, 4018
BseYI CCCAGC 1 cut(s) 1160
BsgI GTGCAG 3 cut(s) 1878, 2643, 4020
Bsh1285I CGRYCG 1 cut(s) 1534
BshFI GGCC 8 cut(s) 249, 865, 877, 1136, 1181, 1798, 3129, 3920
BshNI GGYRCC 3 cut(s) 395, 1994, 3952
BsiEI CGRYCG 1 cut(s) 1534
BsiHKAI GWGCWC 4 cut(s) 1493, 2444, 2704, 3139
BsiSI CCGG 3 cut(s) 168, 2599, 2665
BslFI GGGAC 7 cut(s) 226, 926, 1231, 1514, 2542, 3728, 3920
BslI CCNNNNNNNGG 5 cut(s) 237, 1100, 2547, 2859, 4058
BsmAI GTCTC 9 cut(s) 230, 307, 435, 780, 851, 1586, 2412, 3699, 4020
BsmBI CGTCTC 1 cut(s) 307
BsmFI GGGAC 7 cut(s) 226, 926, 1231, 1514, 2542, 3728, 3920
BsmI GAATGC 1 cut(s) 155
BsnI GGCC 8 cut(s) 249, 865, 877, 1136, 1181, 1798, 3129, 3920
Bso31I GGTCTC 2 cut(s) 230, 1586
Bsp119I TTCGAA 1 cut(s) 2782
Bsp1286I GDGCHC 5 cut(s) 1106, 1493, 2444, 2704, 3139
Bsp13I TCCGGA 1 cut(s) 2664
Bsp1407I TGTACA 3 cut(s) 2197, 2556, 2677
BspACI CCGC 8 cut(s) 45, 160, 222, 273, 329, 429, 761, 3457
BspANI GGCC 8 cut(s) 249, 865, 877, 1136, 1181, 1798, 3129, 3920
BspCNI CTCAG 4 cut(s) 1505, 3940, 3967, 4171
BspEI TCCGGA 1 cut(s) 2664
BspHI TCATGA 2 cut(s) 1549, 2976
BspLI GGNNCC 8 cut(s) 242, 321, 397, 1105, 1996, 3021, 3163, 3954
BspMI ACCTGC 3 cut(s) 1622, 2055, 2830
BspPI GGATC 4 cut(s) 1003, 1287, 1876, 2532
BspT104I TTCGAA 1 cut(s) 2782
BspT107I GGYRCC 3 cut(s) 395, 1994, 3952
BspTI CTTAAG 1 cut(s) 1198
BspTNI GGTCTC 2 cut(s) 230, 1586
BsrBI CCGCTC 2 cut(s) 275, 331
BsrDI GCAATG 4 cut(s) 385, 1975, 2293, 2808
BsrFI RCCGGY 2 cut(s) 167, 2598
BsrGI TGTACA 3 cut(s) 2197, 2556, 2677
BsrI ACTGG 8 cut(s) 469, 952, 1137, 2517, 2695, 3273, 3561, 4019
BssAI RCCGGY 2 cut(s) 167, 2598
BssECI CCNNGG 5 cut(s) 244, 391, 924, 1720, 3522
BssNI GRCGYC 4 cut(s) 291, 378, 396, 531
BssT1I CCWWGG 2 cut(s) 244, 3522
Bst2UI CCWGG 5 cut(s) 393, 925, 1722, 3024, 4028
Bst6I CTCTTC 2 cut(s) 32, 310
BstACI GRCGYC 4 cut(s) 291, 378, 396, 531
BstAFI CTTAAG 1 cut(s) 1198
BstAPI GCANNNNNTGC 1 cut(s) 3548
BstAUI TGTACA 3 cut(s) 2197, 2556, 2677
BstBAI YACGTR 3 cut(s) 2792, 3341, 4058
BstBI TTCGAA 1 cut(s) 2782
BstDEI CTNAG 5 cut(s) 1052, 1492, 3948, 3975, 4158
BstENI CCTNNNNNAGG 1 cut(s) 2857
BstH2I RGCGCY 2 cut(s) 399, 2911
BstHHI GCGC 2 cut(s) 398, 2910
BstMAI GTCTC 9 cut(s) 230, 307, 435, 780, 851, 1586, 2412, 3699, 4020
BstMCI CGRYCG 1 cut(s) 1534
BstMWI GCNNNNNNNGC 6 cut(s) 44, 152, 168, 337, 3463, 3548
BstNI CCWGG 5 cut(s) 393, 925, 1722, 3024, 4028
BstNSI RCATGY 8 cut(s) 833, 2280, 2300, 2526, 2598, 3111, 3173, 3810
BstSCI CCNGG 5 cut(s) 391, 923, 1720, 3022, 4026
BstSFI CTRYAG 6 cut(s) 1024, 1095, 1221, 2265, 2880, 2922
BstSLI GKGCMC 2 cut(s) 2444, 3139
BstSNI TACGTA 1 cut(s) 2792
BstV1I GCAGC 7 cut(s) 753, 2212, 2409, 2468, 2519, 2930, 4018
BstV2I GAAGAC 2 cut(s) 272, 3400
BstX2I RGATCY 6 cut(s) 1008, 1477, 2537, 2572, 3214, 3912
BstYI RGATCY 6 cut(s) 1008, 1477, 2537, 2572, 3214, 3912
BsuI GTATCC 2 cut(s) 538, 1597
BsuRI GGCC 8 cut(s) 249, 865, 877, 1136, 1181, 1798, 3129, 3920
BtsI GCAGTG 2 cut(s) 2704, 3261
BveI ACCTGC 3 cut(s) 1622, 2055, 2830
CaiI CAGNNNCTG 4 cut(s) 1637, 2690, 2921, 4133
CciI TCATGA 2 cut(s) 1549, 2976
CfoI GCGC 2 cut(s) 398, 2910
Cfr10I RCCGGY 2 cut(s) 167, 2598
Cfr13I GGNCC 4 cut(s) 22, 240, 1796, 3162
CseI GACGC 7 cut(s) 161, 386, 539, 2528, 2939, 3388, 3829
DdeI CTNAG 5 cut(s) 1052, 1492, 3948, 3975, 4158
DinI GGCGCC 1 cut(s) 397
DraI TTTAAA 1 cut(s) 1705
DraIII CACNNNGTG 1 cut(s) 1607
EaeI YGGCCR 4 cut(s) 863, 1179, 3127, 3918
Eam1104I CTCTTC 2 cut(s) 32, 310
EarI CTCTTC 2 cut(s) 32, 310
Ecl136II GAGCTC 1 cut(s) 1491
Eco105I TACGTA 1 cut(s) 2792
Eco130I CCWWGG 2 cut(s) 244, 3522
Eco147I AGGCCT 1 cut(s) 249
Eco24I GRGCYC 2 cut(s) 1106, 1493
Eco31I GGTCTC 2 cut(s) 230, 1586
Eco47I GGWCC 3 cut(s) 22, 240, 3162
Eco53kI GAGCTC 1 cut(s) 1491
Eco57I CTGAAG 6 cut(s) 1287, 1937, 2412, 2888, 3101, 3846
Eco72I CACGTG 2 cut(s) 3341, 4058
EcoICRI GAGCTC 1 cut(s) 1491
EcoNI CCTNNNNNAGG 1 cut(s) 2857
EcoRI GAATTC 2 cut(s) 1786, 2848
EcoRII CCWGG 5 cut(s) 391, 923, 1720, 3022, 4026
EcoT14I CCWWGG 2 cut(s) 244, 3522
EcoT22I ATGCAT 4 cut(s) 2278, 3171, 3364, 3607
EcoT38I GRGCYC 2 cut(s) 1106, 1493
EgeI GGCGCC 1 cut(s) 397
EheI GGCGCC 1 cut(s) 397
ErhI CCWWGG 2 cut(s) 244, 3522
Esp3I CGTCTC 1 cut(s) 307
FaqI GGGAC 7 cut(s) 226, 926, 1231, 1514, 2542, 3728, 3920
FauI CCCGC 1 cut(s) 436
FauNDI CATATG 1 cut(s) 679
FbaI TGATCA 1 cut(s) 1552
FblI GTMKAC 2 cut(s) 150, 2928
Fnu4HI GCNGC 9 cut(s) 329, 767, 2226, 2423, 2457, 2508, 2919, 4032, 4195
FriOI GRGCYC 2 cut(s) 1106, 1493
Fsp4HI GCNGC 9 cut(s) 329, 767, 2226, 2423, 2457, 2508, 2919, 4032, 4195
FspBI CTAG 6 cut(s) 497, 609, 1475, 2336, 3152, 4043
GlaI GCGC 2 cut(s) 397, 2909
GluI GCNGC 9 cut(s) 329, 767, 2226, 2423, 2457, 2508, 2919, 4032, 4195
GsaI CCCAGC 1 cut(s) 1164
GsuI CTGGAG 3 cut(s) 2302, 2534, 4002
HaeII RGCGCY 2 cut(s) 399, 2911
HaeIII GGCC 8 cut(s) 249, 865, 877, 1136, 1181, 1798, 3129, 3920
HapII CCGG 3 cut(s) 168, 2599, 2665
HgaI GACGC 7 cut(s) 161, 386, 539, 2528, 2939, 3388, 3829
HhaI GCGC 2 cut(s) 398, 2910
Hin1I GRCGYC 4 cut(s) 291, 378, 396, 531
Hin6I GCGC 2 cut(s) 396, 2908
HinP1I GCGC 2 cut(s) 396, 2908
HincII GTYRAC 7 cut(s) 151, 910, 1243, 1398, 1756, 3198, 3391
HindII GTYRAC 7 cut(s) 151, 910, 1243, 1398, 1756, 3198, 3391
HindIII AAGCTT 3 cut(s) 2900, 3099, 3445
HpaI GTTAAC 2 cut(s) 1398, 1756
HpaII CCGG 3 cut(s) 168, 2599, 2665
Hpy99I CGWCG 3 cut(s) 152, 155, 185
HpyF10VI GCNNNNNNNGC 6 cut(s) 44, 152, 168, 337, 3463, 3548
HpyF3I CTNAG 5 cut(s) 1052, 1492, 3948, 3975, 4158
Hsp92I GRCGYC 4 cut(s) 291, 378, 396, 531
HspAI GCGC 2 cut(s) 396, 2908
KasI GGCGCC 1 cut(s) 395
Kpn2I TCCGGA 1 cut(s) 2664
KpnI GGTACC 1 cut(s) 1998
KroI GCCGGC 2 cut(s) 167, 2598
KroNI GCCGGC 2 cut(s) 169, 2600
Ksp22I TGATCA 1 cut(s) 1552
KspAI GTTAAC 2 cut(s) 1398, 1756
LmnI GCTCC 9 cut(s) 266, 1109, 1488, 2086, 2283, 2305, 2798, 3351, 3752
Lsp1109I GCAGC 7 cut(s) 753, 2212, 2409, 2468, 2519, 2930, 4018
MaeI CTAG 6 cut(s) 497, 609, 1475, 2336, 3152, 4043
MbiI CCGCTC 2 cut(s) 275, 331
MfeI CAATTG 2 cut(s) 235, 3747
MflI RGATCY 6 cut(s) 1008, 1477, 2537, 2572, 3214, 3912
MhlI GDGCHC 5 cut(s) 1106, 1493, 2444, 2704, 3139
MlsI TGGCCA 4 cut(s) 865, 1181, 3129, 3920
MluNI TGGCCA 4 cut(s) 865, 1181, 3129, 3920
Mly113I GGCGCC 1 cut(s) 396
MlyI GAGTC 1 cut(s) 3203
MmeI TCCRAC 4 cut(s) 607, 2142, 3265, 3354
Mox20I TGGCCA 4 cut(s) 865, 1181, 3129, 3920
Mph1103I ATGCAT 4 cut(s) 2278, 3171, 3364, 3607
MroI TCCGGA 1 cut(s) 2664
MroNI GCCGGC 2 cut(s) 167, 2598
MroXI GAANNNNTTC 4 cut(s) 1422, 2852, 3831, 4149
MscI TGGCCA 4 cut(s) 865, 1181, 3129, 3920
MslI CAYNNNNRTG 2 cut(s) 465, 2469
Msp20I TGGCCA 4 cut(s) 865, 1181, 3129, 3920
MspA1I CMGCKG 3 cut(s) 2921, 3947, 4197
MspCI CTTAAG 1 cut(s) 1198
MspI CCGG 3 cut(s) 168, 2599, 2665
MspR9I CCNGG 5 cut(s) 393, 925, 1722, 3024, 4028
MunI CAATTG 2 cut(s) 235, 3747
Mva1269I GAATGC 1 cut(s) 155
MvaI CCWGG 5 cut(s) 393, 925, 1722, 3024, 4028
MwoI GCNNNNNNNGC 6 cut(s) 44, 152, 168, 337, 3463, 3548
NaeI GCCGGC 2 cut(s) 169, 2600
NarI GGCGCC 1 cut(s) 396
NdeI CATATG 1 cut(s) 679
NgoMIV GCCGGC 2 cut(s) 167, 2598
NlaIV GGNNCC 8 cut(s) 242, 321, 397, 1105, 1996, 3021, 3163, 3954
NmuCI GTSAC 8 cut(s) 1456, 1516, 1607, 1915, 2238, 2500, 3256, 4096
NsiI ATGCAT 4 cut(s) 2278, 3171, 3364, 3607
NspI RCATGY 8 cut(s) 833, 2280, 2300, 2526, 2598, 3111, 3173, 3810
NspV TTCGAA 1 cut(s) 2782
OliI CACNNNNGTG 1 cut(s) 465
PaeI GCATGC 2 cut(s) 2280, 2598
PagI TCATGA 2 cut(s) 1549, 2976
PceI AGGCCT 1 cut(s) 249
PciI ACATGT 1 cut(s) 3107
PcsI WCGNNNNNNNCGW 2 cut(s) 297, 2788
PctI GAATGC 1 cut(s) 155
PdiI GCCGGC 2 cut(s) 169, 2600
PdmI GAANNNNTTC 4 cut(s) 1422, 2852, 3831, 4149
PflMI CCANNNNNTGG 1 cut(s) 4058
PfoI TCCNGGA 1 cut(s) 3022
PkrI GCNGC 9 cut(s) 330, 768, 2227, 2424, 2458, 2509, 2920, 4033, 4196
Ple19I CGATCG 1 cut(s) 1534
PleI GAGTC 1 cut(s) 3202
PluTI GGCGCC 1 cut(s) 399
PmaCI CACGTG 2 cut(s) 3341, 4058
PmlI CACGTG 2 cut(s) 3341, 4058
PpsI GAGTC 1 cut(s) 3202
Ppu21I YACGTR 3 cut(s) 2792, 3341, 4058
PscI ACATGT 1 cut(s) 3107
PshBI ATTAAT 1 cut(s) 3222
Psp124BI GAGCTC 1 cut(s) 1493
Psp1406I AACGTT 2 cut(s) 2220, 3371
Psp6I CCWGG 5 cut(s) 391, 923, 1720, 3022, 4026
PspCI CACGTG 2 cut(s) 3341, 4058
PspFI CCCAGC 1 cut(s) 1160
PspGI CCWGG 5 cut(s) 391, 923, 1720, 3022, 4026
PspN4I GGNNCC 8 cut(s) 242, 321, 397, 1105, 1996, 3021, 3163, 3954
PspPI GGNCC 4 cut(s) 22, 240, 1796, 3162
PstNI CAGNNNCTG 4 cut(s) 1637, 2690, 2921, 4133
PsuI RGATCY 6 cut(s) 1008, 1477, 2537, 2572, 3214, 3912
PvuI CGATCG 1 cut(s) 1534
PvuII CAGCTG 3 cut(s) 2921, 3947, 4197
RseI CAYNNNNRTG 2 cut(s) 465, 2469
SacI GAGCTC 1 cut(s) 1493
SalI GTCGAC 1 cut(s) 149
SatI GCNGC 9 cut(s) 329, 767, 2226, 2423, 2457, 2508, 2919, 4032, 4195
Sau96I GGNCC 4 cut(s) 22, 240, 1796, 3162
SchI GAGTC 1 cut(s) 3203
ScrFI CCNGG 5 cut(s) 393, 925, 1722, 3024, 4028
SduI GDGCHC 5 cut(s) 1106, 1493, 2444, 2704, 3139
SfcI CTRYAG 6 cut(s) 1024, 1095, 1221, 2265, 2880, 2922
SfoI GGCGCC 1 cut(s) 397
SfuI TTCGAA 1 cut(s) 2782
SgrDI CGTCGACG 1 cut(s) 149
SinI GGWCC 3 cut(s) 22, 240, 3162
SmiMI CAYNNNNRTG 2 cut(s) 465, 2469
SmlI CTYRAG 3 cut(s) 1198, 2589, 2832
SmoI CTYRAG 3 cut(s) 1198, 2589, 2832
SnaBI TACGTA 1 cut(s) 2792
SpeI ACTAGT 1 cut(s) 3151
SphI GCATGC 2 cut(s) 2280, 2598
SseBI AGGCCT 1 cut(s) 249
SsiI CCGC 8 cut(s) 45, 160, 222, 273, 329, 429, 761, 3457
SspDI GGCGCC 1 cut(s) 395
SspI AATATT 3 cut(s) 1392, 1651, 1699
SspMI CTAG 6 cut(s) 497, 609, 1475, 2336, 3152, 4043
SstI GAGCTC 1 cut(s) 1493
StuI AGGCCT 1 cut(s) 249
StyD4I CCNGG 5 cut(s) 391, 923, 1720, 3022, 4026
StyI CCWWGG 2 cut(s) 244, 3522
TaqI TCGA 9 cut(s) 78, 150, 751, 1808, 2704, 2782, 3203, 3411, 4072
TatI WGTACW 4 cut(s) 2197, 2556, 2677, 2941
TauI GCSGC 1 cut(s) 331
TseFI GTSAC 8 cut(s) 1456, 1516, 1607, 1915, 2238, 2500, 3256, 4096
TseI GCWGC 8 cut(s) 766, 2225, 2422, 2456, 2507, 2918, 4031, 4194
Tsp45I GTSAC 8 cut(s) 1456, 1516, 1607, 1915, 2238, 2500, 3256, 4096
TspGWI ACGGA 4 cut(s) 35, 169, 369, 798
Van91I CCANNNNNTGG 1 cut(s) 4058
Vha464I CTTAAG 1 cut(s) 1198
VneI GTGCAC 2 cut(s) 2440, 3135
VpaK11BI GGWCC 3 cut(s) 22, 240, 3162
VspI ATTAAT 1 cut(s) 3222
XagI CCTNNNNNAGG 1 cut(s) 2857
XapI RAATTY 9 cut(s) 515, 566, 880, 1523, 1544, 1786, 2848, 3141, 3830
XceI RCATGY 8 cut(s) 833, 2280, 2300, 2526, 2598, 3111, 3173, 3810
XcmI CCANNNNNNNNNTGG 2 cut(s) 2956, 3753
XmiI GTMKAC 2 cut(s) 150, 2928
XmnI GAANNNNTTC 4 cut(s) 1422, 2852, 3831, 4149
XspI CTAG 6 cut(s) 497, 609, 1475, 2336, 3152, 4043
ZraI GACGTC 1 cut(s) 292
Zsp2I ATGCAT 4 cut(s) 2278, 3171, 3364, 3607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.