Rorug03G0263400

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0003619
Physical Location & Seq
Forward (+)
25508862 .. 25532368
23507 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0263400.1

Sequence Viewer

Length: 5664 bp
ATGGGGACCGGAACGAGACAGCTCAAGGCTATGCTTCGCAAGAATTGGCTTCTCAAAATTCGTCACCCTTTCGTCACCTGCGCTGAGATATTACTCCCCACTGTAGTAATGTTGATGCTCATAGCTGTAAGGACACATGTTGATACGCAGATTCACCCTTCACAGCCATATATCCGGAAAGGGATGTTGGTGGAAGTGGGCAAAGGAATATCCCCTAATTTTGAGCAAGTGTTGGAATTACTATTGAAGAAGGAAGAGATTTTGGCTTTTGCACCAGACACAAAAGAGACAAGGTCAATGATCAACGTGATGTCGATGAAATTTCCTCTCTTGAAGCATGTTTCTAGAGTTTACAAAGATGAGCAGGAGCTGGAAACCTATATACGCTCAAATCTCTATGGTACCTGCAATCAAATCATGAATTGCTCGAACCCAAAAATCAAAGGAGCAGTAGTATTTCACGATCAAGGTCCTCAACTTTTCGATTATAGCATACGTCTTAATCACACATGGGCTTTCTCGGGATTTCCTGATGTTAAATCGATCATGGACACAAATGGTCCTTACTTAAATGACTTGGAACTGGGCGTGAGTACTGTACCGACAATGCAATACAGCTGCAGTGGATTTTTAACTCTCCAGCAAGCACTAGACTCTTTTATAATTTTTGCGGCCCAGCAATCGGATACCAGAAATATAGAACTTCCTACTTCGCTGTCCTCTAGCACACCTTCCTCTCTCAAAGTTCCATGGACGCGATATGGTCCGTCAAACATCCGTATAGTCCCGTTTCCAACTCGTGAATACACTGATGATGAGTTCCAGTCCATCATAAAAAGTGTCATGGGTGTACTGTATCTTTTGGGATTTCTCTATCCAATTTCGCGCTTGATCAGCTATTCTGTGTTTGAGAAGGAACAAAAGATAAGAGAAGGTCTTTATATGATGGGGTTGAAAGATGGGGTATTCCATCTATCGTGGTTCATTGCATATGCTTTGCAGTTCGCAGTTTCTTCTCTTATTATTACAGTTTGCACGATGGATAACCTTTTCAAGTATAGTGACAAGTCAGTGGTGTTCATTTACTTCTTCTTTTTCGGAATAAGCGCGATTATGCTGTCATTTTTAATCTCTACATTCTTCGAACGAGCGAAGACAGCGGTTGCGGTTGGGACCCTTGCTTTCTTGGGTGCTTTTTTCCCATACTACTCTGTCAATGACGAGTCTGTTCCTATGATTCTAAAGGTCATTGCCTCTCTGCTTTCACCTACGGCTTTTGCTCTGGGGTCAATCAACTTTGCTGATTATGAGCGTGCTCATGTTGGCCTTCGTTGGAGCAATATATGGCGGGCATCATCCGGAGTGAATTTTTCAGTCTGTCTCCTGATGATGTTGCTCGATGCGTTGCTGTACTGTGTAATTGGTCTTTACCTAGATAAGGTACTTCCGAGAGAGAATGGAGTTAGGTATCCCTGGAACTTCATTTTTCAGAAGTGCTTTTGGAAGAATCCAAATATTAACAAACACCACAACTCCAGTCCAGAAGTTCATATTCGTGACAAGGTATCCCAGAAGGCAATGTTTTCTCGGAAAGAAAATGCTAAAGCTGCTGTGGAAGCAATAACGTTCGATATGAGACAACAAGAGCTTGACCATAGATGCATCCAAATAAGGAATCTGCATAAGGTTTATGCTAGTAAAAAGGGGAAATGTTGTGCTGTCAACTCATTACAGCTCACCATGTATGAAAATCAGATTCTTGCTCTTCTAGGACATAATGGAGCTGGTAAAAGCACAACGATATCAATGCTTGTTGGCCTTCTTCGTCCTACTTCAGGGGATGCTATGGTGTTTGGAAAAAATATTATGACAGACATGGAGGAGATACGGAAAGAGTTGGGTGTATGCCCTCAGCATGATATCCTTTTTCCAGAATTGACGGTGAAGGAGCACTTGGAAATATTTGCTATATTGAAAGGTGTGCAGGAAGATTTTCTGAACAGCGTTGTTATTGATATGGTTGACCAAGTAGGGTTGGCCGATAAAATCAACACTGCTGTTATGGCTCTTTCTGGAGGAATGAAGAGGAAGTTGTCTCTTGGAATTGCACTAATTGGAAATAGTAAGGTTATAATTCTTGATGAACCTACTAGTGGAATGGATCCATACTCTATGCGCTTGACTTGGCAATTAATAAAAAAGATTAGAAAGGGCAGGATAGTATTACTGACAACACACTCAATGGATGAAGCTGAGGCACTTGGAGATCGCATAGCCATCATGGCCAATGGTTCTTTGAAATGCTGCGGAAGTTCCCTTTTCTTGAAGCATCAGTATGGGGTTGGTTATACTCTAACTCTAGTGAAGTCTGCACCCACTGCCTCTATGGCTGCTGATATTGTTTACCGCCATATCCCATCAGCAACATGTGTGAGTGAGGTTGGAACTGAGATTTCCTTTAAGCTTCCATTGGCATCCTCAACATCCTTCGAAAGTATGTTTAGAGAAATTGAGAGTTGCATGGGATCATCCACATCAAACTTAGAAACAAGTTGTGATGAGAAGGACTATATTGGCATTGAAAGTTATGGCATATCTGTCACAACTTTGGAGGAGGTGTTCCTGAGGGTTGCAGGATGCGATTATGACGAAGCTGCTTGTTTTGACCAGAAGAATGGTCCACTGTGCCCTGAGTCTCTGATTTCTCAAACTTCTCATGATCCTACCCACAAACAGATTCTTCACTCGAAGAAATCTTTTTCTTATTATAAAGAGATTCTTGGGGTCTTATTTACAATGGTGGGAAGAGCCTGTGGTTTAATTTTTGCTACAGTGTTAAGTTTCTTAAACTTCTTAGGTGTGCAATGCTGTGGCTGTTGTATCATTTCAAGGTCAACATTTTGGAGGCATTCTAAAGCATTATTCAAAAAGAGGGCAATATCTGCCCGCAGAGACCGTAAAACAATTGTTTTCCAGCTTGTAATTCCTGCAGTTTTCTTATTTTTTGGTCTTTTATTTCTCAAGCTCAAGCCACATCCTGATCAGGAGTCTGTTACCTTCACAACTTCACATTTTAATCCACTATTACGTGGTGGTGGTGGTGGTGGTCCAATTCCTTTTGATCTATCCCGGCCTATTGCACAGGAGGTTGCAGAATACATAGAAGGGGGTTGGATACAAACCTTCAAACCTAGTGGGTATAAATTCCCCAACTCAGAGAAGGCATTGAATGATGCAATTGAGGTAGCAGGGGAAACTCTGGGACCTGCTTTACTTTCAATGAGTGAATTTTTGATGTCCAGCTTTAATGAATCTTACCAGTCAAGGTATGGCGCAGTTGTGATGGATGATCAGAGTGATGATGGGAGTTTAGGGTACACTGTGCTACACAACAGTTCCTGTCAGCATGCTGCCCCAACCTTTATCAATTTAGTAAATGCTGCAATTCTTCGGCTTGCTTCTCGTAATAAGAATGTGACAATTCAAACACGCAATCACCCTTTGCCAATGACAAAAAGTCAACATCTGCAACGGCATGATTTGGATGCTTTCTCTGCTGCAGTTATTGTTAGTATCGCCTTCTCCTTCATTCCTGCGTCATTTGCTGTTCCTATAGTGAAGGAACGTGAAGTAAAAGCTAAACACCAACAACTTATTAGTGGAGTTTCCGTACTGTCATATTGGACTTCTACATACATATGGGACTTCATCAGTTTCCTCTTTCCTTCATCTTTTGCCATAGTTCTTTTTTACATATTTGGTCTGGACCAGTTTATTGGAAGGGGATGTTTACTGTCGACTGTTGTCATGTTTTTAGCATACGGATTAGCAATTGCATCATCAACATACTGCCTTACGTTTTTCTTTTCTGATCACACTATGGCTCAGAATGTGGTTCTCTTGGTCCACTTTTTCACGGGACTCATTCTTATGGTTATCTCATTCATTATGGGGCTTATAAAGACGACGACAAGTGCAAATTCTTTTCTTAAGAACTTTTTCAGATTATCTCCAGGATTTTGCTTTGCTGACGGACTCGCTTCACTGGCTCTTCTACGTCAAGATATGAAAAATAAAAGCAATAAGGCCTTTGATTGGAATGTTACTGGTGGCTCAATCTGCTACCTTTGTATTGAGAGCATTTGTTACTTCCTTTTGGCACTTGGTCTTGAAATCTTGCCTTTCAATAAATTGACACTAGCCACACTCAAGGAGTGGTGGAAGAGTATTAAAAGTATCCCACCTAGCACTTCAAGTTATCGAGAGCCCCTTCTGACTTCTTCCGCAGAATCTGTCACTCTGGACCTTGATGAGGACACAGATGTGAAGGCAGAAAGAACTAGGGTACTTTCAGGTTCCATTGACAATGCCATCATCTACTTGCGTAATCTTCGTAAGGTGTATCCAGGAGGACAGCAGCATGCTACAAAGGTTGCGGTACATTCATTGACTTTTTCAGTTCAAGAAGGAGAATGTTTCGGTTTTTTAGGAACAAATGGAGCTGGGAAGACCACAACTCTGTCTATGTTGACTGGTGAGGAATCTCCTACGGATGGGACTGCTTGTATATTTGGCAAGGATATTTCCTCCAATCCCAAGGCTGCTCGTCAACATATTGGGTTTTGCCCACAGTTTGATGCTCTGTTAGAATATTTAACTGTTCAAGAGCATCTTGAGCTTTATGCCACGATAAAAGGAGTACCAGATAATAAGATAGCTGAGGTTGTAATGGAAAAGTTGGTGGAGTTTGACTTGTTAAAGCATGCCAGCAAACCATCATTTTCCCTCAGTGGGGGAAACAAGCGAAAATTATCTGTTGCAATTGCTATGATTGGAGATCCTCCTATAGTTATTCTTGATGAACCATCAACAGGTATGGATCCAATTGCCAAAAGATTCATGTGGGAAGTGATATCTCGTTTATCGACCAGACGAGGAAAGACAGCAGTGATACTGACTACCCATAGTATGAATGAAGCTCAAGCTCTGTGCACCCGTATCGGAATCATGGTTGGAGGCCAATTACGGTGCATTGGAAGTCCCCAACATTTAAAAACTCGATTTGGAAATCATCTTGAGCTAGAGGTTAAACCTATTGAAGTCAGCTCTGTGGACTTGGACAAGCTTTGCCGAGTTATCCAAGAAAGCCTCTCATATGTTCCTTCTCATCCAATGAGCTTACTTGATGGCCTTGAAGTCTGCATTGGTGCCACTGACTCCATTGTGGCAGAAAATGCATCGGTGGCAGAGATTAGTTTATCACGAGAAATGATAATTATGATTGGACGATGGCTTGGGAATGAAGAAAGAATAAAGCCGCTTATATCTTCAACACCACTTTCAGATGGGGTGCTTGGTGAACAGTTATTTGAGCAGTTAGATCGAGATGGTGGGATACCGTTGCCAATATTTTCTGAATGGTGGCTGTTCAATGACAAGTTTTCAGCAATCGATTCATTTGTCCTATCATCATTTCCTGGTGCAATGTTCCAGGGAATGAATGGCTTGAGTGTTAAATATCAGTTGCCTTGTGGTCCGCACCTCTCTCTTGCTGATGTATTTGGACACCTTGAACGGAAAAGAAACCGCCTTGGCATCGCAGAGTACAGTATTAGCCAGTCCACTCTGGAGAGCATATTCAATCATTTTGCAGCCAACTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

1887

Amino Acids

209.91

Weight (kDa)

7.75

Isoelectric Point (pI)

35.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC2_membrane_3 PF12698 211 - 476 3.7e-20 ABC-2 family transporter protein
ABC_tran PF00005 575 - 718 1.3e-30 ABC transporter
ABC2_membrane_3 PF12698 987 - 1388 9e-41 ABC-2 family transporter protein
ABC_tran PF00005 1481 - 1623 3.9e-28 ABC transporter
AAA_21 PF13304 1592 - 1658 9.2e-09 AAA domain, putative AbiEii toxin, Type IV TA system
R1_ABCA1 PF23321 1813 - 1873 1e-10 ABCA1-like, C-terminal R1 regulatory domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 662, 2132, 2769, 3959
AarI CACCTGC 1 cut(s) 86
Acc36I ACCTGC 3 cut(s) 86, 413, 3274
Acc65I GGTACC 1 cut(s) 401
AccB1I GGYRCC 2 cut(s) 401, 5207
AccI GTMKAC 1 cut(s) 3797
AccII CGCG 3 cut(s) 757, 886, 1109
AccIII TCCGGA 2 cut(s) 174, 1358
AclI AACGTT 1 cut(s) 1625
AclWI GGATC 7 cut(s) 2156, 2169, 2533, 2714, 4832, 4874, 4887
AcoI YGGCCR 2 cut(s) 2037, 2283
AcsI RAATTY 6 cut(s) 57, 320, 1366, 3203, 3287, 3979
AcuI CTGAAG 1 cut(s) 1818
AflII CTTAAG 1 cut(s) 3989
AflIII ACRYGT 2 cut(s) 136, 2426
AhdI GACNNNNNGTC 1 cut(s) 3516
AhlI ACTAGT 1 cut(s) 2150
AjnI CCWGG 5 cut(s) 1472, 4012, 4405, 5476, 5490
AjuI GAANNNNNNNTTGG 6 cut(s) 170, 202, 1937, 1969, 5187, 5219
AleI CACNNNNGTG 1 cut(s) 4322
AloI GAACNNNNNNTCC 2 cut(s) 2603, 2635
Alw21I GWGCWC 3 cut(s) 1318, 1953, 4994
Alw26I GTCTC 7 cut(s) 10, 281, 1385, 1630, 2100, 2700, 2946
Alw44I GTGCAC 1 cut(s) 4990
AlwI GGATC 7 cut(s) 2156, 2169, 2533, 2714, 4832, 4874, 4887
AlwNI CAGNNNCTG 3 cut(s) 370, 3399, 4292
Ama87I CYCGRG 1 cut(s) 520
Aor13HI TCCGGA 2 cut(s) 174, 1358
AoxI GGCC 9 cut(s) 672, 1324, 1816, 2037, 2283, 3131, 4086, 5017, 5188
ApaLI GTGCAC 1 cut(s) 4990
ApoI RAATTY 6 cut(s) 57, 320, 1366, 3203, 3287, 3979
AseI ATTAAT 1 cut(s) 2192
Asp700I GAANNNNTTC 2 cut(s) 1992, 3998
Asp718I GGTACC 1 cut(s) 401
AspLEI GCGC 5 cut(s) 83, 888, 1109, 2178, 3335
AsuC2I CCSGG 1 cut(s) 3130
AsuHPI GGTGA 9 cut(s) 56, 67, 146, 1257, 1729, 1954, 3488, 4547, 5369
AsuII TTCGAA 2 cut(s) 1143, 2490
AvaI CYCGRG 1 cut(s) 520
AxyI CCTNAGG 1 cut(s) 2624
BaeGI GKGCMC 2 cut(s) 2690, 4994
BalI TGGCCA 1 cut(s) 2285
BamHI GGATCC 2 cut(s) 2161, 4879
BanI GGYRCC 2 cut(s) 401, 5207
BanII GRGCYC 1 cut(s) 4269
BauI CACGAG 2 cut(s) 798, 5262
BbsI GAAGAC 2 cut(s) 1160, 4514
Bbv12I GWGCWC 3 cut(s) 1318, 1953, 4994
BbvCI CCTCAGC 3 cut(s) 1911, 2253, 4719
BceAI ACGGC 2 cut(s) 1287, 3548
BcgI CGANNNNNNTGC 6 cut(s) 1789, 1823, 4981, 5015, 5363, 5397
BciT130I CCWGG 5 cut(s) 1474, 4014, 4407, 5478, 5492
BciVI GTATCC 7 cut(s) 679, 1479, 1576, 3168, 4247, 4413, 5388
BclI TGATCA 5 cut(s) 300, 891, 3040, 3349, 3871
BcnI CCSGG 1 cut(s) 3130
BcoDI GTCTC 7 cut(s) 10, 281, 1385, 1630, 2100, 2700, 2946
BcuI ACTAGT 1 cut(s) 2150
BfmI CTRYAG 7 cut(s) 102, 619, 2829, 2988, 3558, 3612, 4845
BfrI CTTAAG 1 cut(s) 3989
BfuAI ACCTGC 3 cut(s) 86, 413, 3274
BfuI GTATCC 7 cut(s) 679, 1479, 1576, 3168, 4247, 4413, 5388
BglI GCCNNNNNGGC 2 cut(s) 2282, 2387
BmcAI AGTACT 1 cut(s) 595
Bme1390I CCNGG 6 cut(s) 1474, 3130, 4014, 4407, 5478, 5492
BmeRI GACNNNNNGTC 1 cut(s) 3516
BmeT110I CYCGRG 1 cut(s) 520
BmiI GGNNCC 9 cut(s) 7, 403, 1174, 1175, 2163, 3264, 4357, 4881, 5209
BmrFI CCNGG 6 cut(s) 1474, 3130, 4014, 4407, 5478, 5492
BmrI ACTGGG 1 cut(s) 593
BmuI ACTGGG 1 cut(s) 593
BoxI GACNNNNGTC 1 cut(s) 3803
BpiI GAAGAC 2 cut(s) 1160, 4514
BpmI CTGGAG 5 cut(s) 623, 1519, 2094, 3996, 5648
Bpu10I CCTNAGC 3 cut(s) 1911, 2253, 4719
Bpu14I TTCGAA 2 cut(s) 1143, 2490
BpuEI CTTGAG 8 cut(s) 8, 3005, 3011, 4193, 4694, 4965, 5096, 5527
BpuMI CCSGG 1 cut(s) 3130
Bsa29I ATCGAT 2 cut(s) 542, 5451
BsaAI YACGTR 1 cut(s) 3089
BsaI GGTCTC 1 cut(s) 2946
BsaJI CCNNGG 5 cut(s) 749, 1472, 4596, 5491, 5590
BsaWI WCCGGW 3 cut(s) 8, 174, 1358
BsaXI ACNNNNNCTCC 8 cut(s) 438, 468, 1517, 1547, 2603, 2633, 3654, 3684
Bse1I ACTGG 9 cut(s) 588, 823, 1536, 3319, 3769, 4050, 4111, 4537, 5617
Bse21I CCTNAGG 1 cut(s) 2624
Bse3DI GCAATG 5 cut(s) 984, 1248, 1584, 2870, 5490
BseAI TCCGGA 2 cut(s) 174, 1358
BseBI CCWGG 5 cut(s) 1474, 4014, 4407, 5478, 5492
BseCI ATCGAT 2 cut(s) 542, 5451
BseDI CCNNGG 5 cut(s) 749, 1472, 4596, 5491, 5590
BseMI GCAATG 5 cut(s) 984, 1248, 1584, 2870, 5490
BseNI ACTGG 9 cut(s) 588, 823, 1536, 3319, 3769, 4050, 4111, 4537, 5617
BseRI GAGGAG 2 cut(s) 1895, 2627
BseSI GKGCMC 2 cut(s) 2690, 4994
BseYI CCCAGC 2 cut(s) 675, 4502
BsgI GTGCAG 2 cut(s) 2003, 2355
Bsh1236I CGCG 3 cut(s) 757, 886, 1109
BshFI GGCC 9 cut(s) 674, 1326, 1818, 2039, 2285, 3133, 4088, 5019, 5190
BshNI GGYRCC 2 cut(s) 401, 5207
BshVI ATCGAT 2 cut(s) 542, 5451
BsiHKAI GWGCWC 3 cut(s) 1318, 1953, 4994
BsiHKCI CYCGRG 1 cut(s) 520
BsiSI CCGG 4 cut(s) 9, 175, 1359, 3130
BslFI GGGAC 8 cut(s) 19, 770, 1186, 3276, 3716, 3933, 4570, 5025
BsmAI GTCTC 7 cut(s) 10, 281, 1385, 1630, 2100, 2700, 2946
BsmFI GGGAC 8 cut(s) 19, 770, 1186, 3276, 3716, 3933, 4570, 5025
BsmI GAATGC 1 cut(s) 2908
BsnI GGCC 9 cut(s) 674, 1326, 1818, 2039, 2285, 3133, 4088, 5019, 5190
Bso31I GGTCTC 1 cut(s) 2946
BsoBI CYCGRG 1 cut(s) 520
Bsp119I TTCGAA 2 cut(s) 1143, 2490
Bsp1286I GDGCHC 5 cut(s) 1318, 1953, 2690, 4269, 4994
Bsp13I TCCGGA 2 cut(s) 174, 1358
Bsp19I CCATGG 1 cut(s) 749
BspANI GGCC 9 cut(s) 674, 1326, 1818, 2039, 2285, 3133, 4088, 5019, 5190
BspDI ATCGAT 2 cut(s) 542, 5451
BspEI TCCGGA 2 cut(s) 174, 1358
BspFNI CGCG 3 cut(s) 757, 886, 1109
BspHI TCATGA 2 cut(s) 417, 2716
BspLI GGNNCC 9 cut(s) 7, 403, 1174, 1175, 2163, 3264, 4357, 4881, 5209
BspMAI CTGCAG 3 cut(s) 623, 2992, 3562
BspMI ACCTGC 3 cut(s) 86, 413, 3274
BspPI GGATC 7 cut(s) 2156, 2169, 2533, 2714, 4832, 4874, 4887
BspQI GCTCTTC 3 cut(s) 1770, 2800, 4056
BspT104I TTCGAA 2 cut(s) 1143, 2490
BspT107I GGYRCC 2 cut(s) 401, 5207
BspTI CTTAAG 1 cut(s) 3989
BspTNI GGTCTC 1 cut(s) 2946
BsrDI GCAATG 5 cut(s) 984, 1248, 1584, 2870, 5490
BsrI ACTGG 9 cut(s) 588, 823, 1536, 3319, 3769, 4050, 4111, 4537, 5617
BssECI CCNNGG 5 cut(s) 749, 1472, 4596, 5491, 5590
BssSI CACGAG 2 cut(s) 798, 5262
BssT1I CCWWGG 3 cut(s) 749, 4596, 5590
Bst2BI CACGAG 2 cut(s) 798, 5262
Bst2UI CCWGG 5 cut(s) 1474, 4014, 4407, 5478, 5492
Bst6I CTCTTC 6 cut(s) 249, 1770, 2078, 2800, 4056, 4217
BstAFI CTTAAG 1 cut(s) 3989
BstAPI GCANNNNNTGC 3 cut(s) 2378, 2942, 5234
BstBAI YACGTR 1 cut(s) 3089
BstBI TTCGAA 2 cut(s) 1143, 2490
BstC8I GCNNGC 9 cut(s) 645, 1314, 1350, 2947, 3408, 3456, 4422, 4764, 4768
BstDSI CCRYGG 1 cut(s) 749
BstENI CCTNNNNNAGG 3 cut(s) 1436, 1833, 4310
BstFNI CGCG 3 cut(s) 757, 886, 1109
BstHHI GCGC 5 cut(s) 83, 888, 1109, 2178, 3335
BstMAI GTCTC 7 cut(s) 10, 281, 1385, 1630, 2100, 2700, 2946
BstNI CCWGG 5 cut(s) 1474, 4014, 4407, 5478, 5492
BstNSI RCATGY 6 cut(s) 140, 341, 2430, 3410, 4424, 4766
BstPAI GACNNNNGTC 1 cut(s) 3803
BstSCI CCNGG 6 cut(s) 1472, 3128, 4012, 4405, 5476, 5490
BstSFI CTRYAG 7 cut(s) 102, 619, 2829, 2988, 3558, 3612, 4845
BstSLI GKGCMC 2 cut(s) 2690, 4994
BstUI CGCG 3 cut(s) 757, 886, 1109
BstV2I GAAGAC 2 cut(s) 1160, 4514
BstX2I RGATCY 3 cut(s) 2161, 4837, 4879
BstXI CCANNNNNNTGG 2 cut(s) 2675, 3776
BstYI RGATCY 3 cut(s) 2161, 4837, 4879
Bsu15I ATCGAT 2 cut(s) 542, 5451
Bsu36I CCTNAGG 1 cut(s) 2624
BsuI GTATCC 7 cut(s) 679, 1479, 1576, 3168, 4247, 4413, 5388
BsuRI GGCC 9 cut(s) 674, 1326, 1818, 2039, 2285, 3133, 4088, 5019, 5190
BsuTUI ATCGAT 2 cut(s) 542, 5451
BtgI CCRYGG 1 cut(s) 749
BtgZI GCGATG 1 cut(s) 5581
BtsI GCAGTG 4 cut(s) 628, 2052, 2376, 4953
BveI ACCTGC 3 cut(s) 86, 413, 3274
Cac8I GCNNGC 9 cut(s) 645, 1314, 1350, 2947, 3408, 3456, 4422, 4764, 4768
CaiI CAGNNNCTG 3 cut(s) 370, 3399, 4292
CciI TCATGA 2 cut(s) 417, 2716
CfoI GCGC 5 cut(s) 83, 888, 1109, 2178, 3335
ClaI ATCGAT 2 cut(s) 542, 5451
CseI GACGC 2 cut(s) 763, 3585
CspCI CAANNNNNGTGG 2 cut(s) 3175, 3210
DraI TTTAAA 1 cut(s) 5052
DriI GACNNNNNGTC 1 cut(s) 3516
EaeI YGGCCR 2 cut(s) 2037, 2283
Eam1104I CTCTTC 6 cut(s) 249, 1770, 2078, 2800, 4056, 4217
Eam1105I GACNNNNNGTC 1 cut(s) 3516
EarI CTCTTC 6 cut(s) 249, 1770, 2078, 2800, 4056, 4217
Eco130I CCWWGG 3 cut(s) 749, 4596, 5590
Eco147I AGGCCT 1 cut(s) 4088
Eco24I GRGCYC 1 cut(s) 4269
Eco31I GGTCTC 1 cut(s) 2946
Eco32I GATATC 3 cut(s) 1803, 1921, 4914
Eco57I CTGAAG 1 cut(s) 1818
Eco81I CCTNAGG 1 cut(s) 2624
Eco88I CYCGRG 1 cut(s) 520
EcoNI CCTNNNNNAGG 3 cut(s) 1436, 1833, 4310
EcoO109I RGGNCCY 3 cut(s) 470, 1173, 3263
EcoRII CCWGG 5 cut(s) 1472, 4012, 4405, 5476, 5490
EcoRV GATATC 3 cut(s) 1803, 1921, 4914
EcoT14I CCWWGG 3 cut(s) 749, 4596, 5590
EcoT22I ATGCAT 2 cut(s) 1664, 5239
EcoT38I GRGCYC 1 cut(s) 4269
ErhI CCWWGG 3 cut(s) 749, 4596, 5590
FalI AAGNNNNNCTT 6 cut(s) 1937, 1969, 2763, 2795, 4656, 4688
FaqI GGGAC 8 cut(s) 19, 770, 1186, 3276, 3716, 3933, 4570, 5025
FauI CCCGC 2 cut(s) 1341, 2954
FauNDI CATATG 3 cut(s) 991, 3698, 5155
FbaI TGATCA 5 cut(s) 300, 891, 3040, 3349, 3871
FblI GTMKAC 1 cut(s) 3797
FriOI GRGCYC 1 cut(s) 4269
GlaI GCGC 5 cut(s) 82, 887, 1108, 2177, 3334
GsaI CCCAGC 2 cut(s) 679, 4506
GsuI CTGGAG 5 cut(s) 623, 1519, 2094, 3996, 5648
HaeIII GGCC 9 cut(s) 674, 1326, 1818, 2039, 2285, 3133, 4088, 5019, 5190
HapII CCGG 4 cut(s) 9, 175, 1359, 3130
HgaI GACGC 2 cut(s) 763, 3585
HhaI GCGC 5 cut(s) 83, 888, 1109, 2178, 3335
Hin6I GCGC 5 cut(s) 81, 886, 1107, 2176, 3333
HinP1I GCGC 5 cut(s) 81, 886, 1107, 2176, 3333
HincII GTYRAC 7 cut(s) 1723, 2023, 2895, 3521, 3798, 4530, 4610
HindII GTYRAC 7 cut(s) 1723, 2023, 2895, 3521, 3798, 4530, 4610
HindIII AAGCTT 2 cut(s) 2462, 5123
HpaII CCGG 4 cut(s) 9, 175, 1359, 3130
HphI GGTGA 9 cut(s) 56, 67, 146, 1257, 1729, 1954, 3488, 4547, 5369
Hpy99I CGWCG 1 cut(s) 3970
HpyCH4IV ACGT 7 cut(s) 306, 496, 1625, 3088, 3625, 3857, 4057
HpySE526I ACGT 7 cut(s) 306, 496, 1625, 3088, 3625, 3857, 4057
HspAI GCGC 5 cut(s) 81, 886, 1107, 2176, 3333
KflI GGGWCCC 1 cut(s) 1173
Kpn2I TCCGGA 2 cut(s) 174, 1358
KpnI GGTACC 1 cut(s) 405
Ksp22I TGATCA 5 cut(s) 300, 891, 3040, 3349, 3871
LguI GCTCTTC 3 cut(s) 1770, 2800, 4056
LmnI GCTCC 6 cut(s) 367, 446, 1335, 1781, 1948, 4499
MaeII ACGT 7 cut(s) 306, 496, 1625, 3088, 3625, 3857, 4057
MfeI CAATTG 5 cut(s) 2964, 3237, 3831, 4821, 4884
MflI RGATCY 3 cut(s) 2161, 4837, 4879
MhlI GDGCHC 5 cut(s) 1318, 1953, 2690, 4269, 4994
MlsI TGGCCA 1 cut(s) 2285
MluNI TGGCCA 1 cut(s) 2285
MlyI GAGTC 7 cut(s) 647, 1232, 2702, 3056, 3915, 4029, 5210
MmeI TCCRAC 6 cut(s) 213, 818, 1313, 2422, 3152, 4993
Mox20I TGGCCA 1 cut(s) 2285
Mph1103I ATGCAT 2 cut(s) 1664, 5239
MroI TCCGGA 2 cut(s) 174, 1358
MroXI GAANNNNTTC 2 cut(s) 1992, 3998
MscI TGGCCA 1 cut(s) 2285
MslI CAYNNNNRTG 5 cut(s) 1554, 2334, 3697, 3929, 4322
Msp20I TGGCCA 1 cut(s) 2285
MspA1I CMGCKG 2 cut(s) 618, 1160
MspCI CTTAAG 1 cut(s) 3989
MspI CCGG 4 cut(s) 9, 175, 1359, 3130
MspR9I CCNGG 6 cut(s) 1474, 3130, 4014, 4407, 5478, 5492
MunI CAATTG 5 cut(s) 2964, 3237, 3831, 4821, 4884
Mva1269I GAATGC 1 cut(s) 2908
MvaI CCWGG 5 cut(s) 1474, 4014, 4407, 5478, 5492
MvnI CGCG 3 cut(s) 757, 886, 1109
NciI CCSGG 1 cut(s) 3130
NcoI CCATGG 1 cut(s) 749
NdeI CATATG 3 cut(s) 991, 3698, 5155
NlaIV GGNNCC 9 cut(s) 7, 403, 1174, 1175, 2163, 3264, 4357, 4881, 5209
NmeAIII GCCGAG 1 cut(s) 5156
NmuCI GTSAC 7 cut(s) 62, 73, 1061, 1556, 2599, 3475, 4294
NsiI ATGCAT 2 cut(s) 1664, 5239
NspI RCATGY 6 cut(s) 140, 341, 2430, 3410, 4424, 4766
NspV TTCGAA 2 cut(s) 1143, 2490
OliI CACNNNNGTG 1 cut(s) 4322
PaeI GCATGC 3 cut(s) 3410, 4424, 4766
PagI TCATGA 2 cut(s) 417, 2716
PaqCI CACCTGC 1 cut(s) 86
PceI AGGCCT 1 cut(s) 4088
PciI ACATGT 2 cut(s) 136, 2426
PciSI GCTCTTC 3 cut(s) 1770, 2800, 4056
PctI GAATGC 1 cut(s) 2908
PdmI GAANNNNTTC 2 cut(s) 1992, 3998
PflFI GACNNNGTC 1 cut(s) 292
PfoI TCCNGGA 2 cut(s) 4012, 4405
PleI GAGTC 7 cut(s) 647, 1231, 2701, 3055, 3915, 4029, 5210
PpsI GAGTC 7 cut(s) 647, 1231, 2701, 3055, 3915, 4029, 5210
Ppu21I YACGTR 1 cut(s) 3089
PpuMI RGGWCCY 3 cut(s) 470, 1173, 3263
PscI ACATGT 2 cut(s) 136, 2426
PshAI GACNNNNGTC 1 cut(s) 3803
PshBI ATTAAT 1 cut(s) 2192
PsiI TTATAA 4 cut(s) 662, 2132, 2769, 3959
Psp1406I AACGTT 1 cut(s) 1625
Psp5II RGGWCCY 3 cut(s) 470, 1173, 3263
Psp6I CCWGG 5 cut(s) 1472, 4012, 4405, 5476, 5490
PspFI CCCAGC 2 cut(s) 675, 4502
PspGI CCWGG 5 cut(s) 1472, 4012, 4405, 5476, 5490
PspN4I GGNNCC 9 cut(s) 7, 403, 1174, 1175, 2163, 3264, 4357, 4881, 5209
PspPPI RGGWCCY 3 cut(s) 470, 1173, 3263
PstI CTGCAG 3 cut(s) 623, 2992, 3562
PstNI CAGNNNCTG 3 cut(s) 370, 3399, 4292
PsuI RGATCY 3 cut(s) 2161, 4837, 4879
PsyI GACNNNGTC 1 cut(s) 292
PvuII CAGCTG 1 cut(s) 618
RseI CAYNNNNRTG 5 cut(s) 1554, 2334, 3697, 3929, 4322
SalI GTCGAC 1 cut(s) 3796
SapI GCTCTTC 3 cut(s) 1770, 2800, 4056
ScaI AGTACT 1 cut(s) 595
SchI GAGTC 7 cut(s) 647, 1232, 2702, 3056, 3915, 4029, 5210
ScrFI CCNGG 6 cut(s) 1474, 3130, 4014, 4407, 5478, 5492
SduI GDGCHC 5 cut(s) 1318, 1953, 2690, 4269, 4994
SfcI CTRYAG 7 cut(s) 102, 619, 2829, 2988, 3558, 3612, 4845
SfuI TTCGAA 2 cut(s) 1143, 2490
SmiMI CAYNNNNRTG 5 cut(s) 1554, 2334, 3697, 3929, 4322
SmlI CTYRAG 9 cut(s) 23, 3020, 3026, 3989, 4208, 4673, 4980, 5075, 5506
SmoI CTYRAG 9 cut(s) 23, 3020, 3026, 3989, 4208, 4673, 4980, 5075, 5506
SpeI ACTAGT 1 cut(s) 2150
SphI GCATGC 3 cut(s) 3410, 4424, 4766
SseBI AGGCCT 1 cut(s) 4088
SspI AATATT 5 cut(s) 1516, 1864, 1962, 4652, 5409
StuI AGGCCT 1 cut(s) 4088
StyD4I CCNGG 6 cut(s) 1472, 3128, 4012, 4405, 5476, 5490
StyI CCWWGG 3 cut(s) 749, 4596, 5590
TaiI ACGT 7 cut(s) 309, 499, 1628, 3091, 3628, 3860, 4060
TatI WGTACW 4 cut(s) 593, 850, 1410, 5604
TauI GCSGC 2 cut(s) 674, 5320
TseFI GTSAC 7 cut(s) 62, 73, 1061, 1556, 2599, 3475, 4294
Tsp45I GTSAC 7 cut(s) 62, 73, 1061, 1556, 2599, 3475, 4294
TspGWI ACGGA 8 cut(s) 756, 767, 1903, 3658, 3837, 4047, 4565, 5590
Tth111I GACNNNGTC 1 cut(s) 292
Vha464I CTTAAG 1 cut(s) 3989
VneI GTGCAC 1 cut(s) 4990
VspI ATTAAT 1 cut(s) 2192
XagI CCTNNNNNAGG 3 cut(s) 1436, 1833, 4310
XapI RAATTY 6 cut(s) 57, 320, 1366, 3203, 3287, 3979
XbaI TCTAGA 1 cut(s) 344
XceI RCATGY 6 cut(s) 140, 341, 2430, 3410, 4424, 4766
XcmI CCANNNNNNNNNTGG 3 cut(s) 2383, 3326, 5498
XmiI GTMKAC 1 cut(s) 3797
XmnI GAANNNNTTC 2 cut(s) 1992, 3998
ZrmI AGTACT 1 cut(s) 595
Zsp2I ATGCAT 2 cut(s) 1664, 5239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.