pycom13g02700

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0003619
Physical Location & Seq
Reverse (-)
1864452 .. 1864919
468 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g02700.1

Sequence Viewer

Length: 468 bp
ATGGTTAAGGCGGCGGAGCTCATGCTGGAGTGTCCCAACACCTTCTTGGCCAACTCGAGGTCTCTCCACATCGGTAGGAGGTTCTCCGCCCTCAGCGCCGACGAGGAGTTGGAGTTTGGGAACGTGTACATCATGTTTCCAATGAGGAGGCTCAGTTCCATAGTCACGGCGGCTGACATGGCGGTGTTCTTCATGGCGGCCAACTCCGCCTCCAAGCGTATATCAGGCGGACAGTTTAGGATCTTGCCGGAGAGTGCCAGTGGAGGAGGAGAAATGGGCGCGGTGGAAAGTGGGAAAGAAAATCTTGGTGATCAAGATCATGAAGGTGATCATCAAGGTCCATTGCGGCTGAGCTTGGACGGAGTAGAGGGGTTGCAAGTGGCGGAGTTTCAGTACAGATTAGCTGCGTGTAGGTCAAGGAAACCGGTGTTGGAAACCATCAAAGAAGAGCAGATTTGTTCTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

156

Amino Acids

16.96

Weight (kDa)

5.43

Isoelectric Point (pI)

53.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 2 - 149 2.2e-25 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 281
AclWI GGATC 1 cut(s) 248
AcoI YGGCCR 2 cut(s) 48, 198
AfaI GTAC 2 cut(s) 128, 395
AfiI CCNNNNNNNGG 1 cut(s) 57
AflIII ACRYGT 1 cut(s) 123
AgeI ACCGGT 1 cut(s) 424
AjuI GAANNNNNNNTTGG 2 cut(s) 413, 445
AloI GAACNNNNNNTCC 2 cut(s) 139, 171
AluBI AGCT 3 cut(s) 19, 354, 404
AluI AGCT 3 cut(s) 19, 354, 404
Alw21I GWGCWC 1 cut(s) 21
Alw26I GTCTC 1 cut(s) 66
AlwI GGATC 1 cut(s) 248
Ama87I CYCGRG 1 cut(s) 55
AoxI GGCC 2 cut(s) 48, 198
ApeKI GCWGC 1 cut(s) 404
AsiGI ACCGGT 1 cut(s) 424
AspLEI GCGC 2 cut(s) 98, 281
AspS9I GGNCC 1 cut(s) 338
AsuHPI GGTGA 2 cut(s) 320, 338
AvaI CYCGRG 1 cut(s) 55
AvaII GGWCC 1 cut(s) 338
BalI TGGCCA 1 cut(s) 50
BanII GRGCYC 1 cut(s) 21
Bbv12I GWGCWC 1 cut(s) 21
BbvCI CCTCAGC 1 cut(s) 92
BbvI GCAGC 1 cut(s) 391
BccI CCATC 1 cut(s) 446
BceAI ACGGC 1 cut(s) 183
BclI TGATCA 2 cut(s) 310, 328
BcoDI GTCTC 1 cut(s) 66
BfaI CTAG 1 cut(s) 462
BfoI RGCGCY 1 cut(s) 99
BisI GCNGC 5 cut(s) 12, 171, 198, 347, 405
BlpI GCTNAGC 1 cut(s) 350
BlsI GCNGC 5 cut(s) 13, 172, 199, 348, 406
Bme18I GGWCC 1 cut(s) 338
BmeT110I CYCGRG 1 cut(s) 55
BmgT120I GGNCC 1 cut(s) 338
BpmI CTGGAG 1 cut(s) 47
Bpu10I CCTNAGC 1 cut(s) 92
Bpu1102I GCTNAGC 1 cut(s) 350
BsaBI GATNNNNATC 1 cut(s) 315
BsaI GGTCTC 1 cut(s) 66
BsaWI WCCGGW 1 cut(s) 424
BsaXI ACNNNNNCTCC 8 cut(s) 98, 128, 139, 169, 194, 224, 377, 407
Bsc4I CCNNNNNNNGG 1 cut(s) 57
Bse118I RCCGGY 1 cut(s) 424
Bse1I ACTGG 1 cut(s) 258
Bse3DI GCAATG 1 cut(s) 341
Bse8I GATNNNNATC 1 cut(s) 315
BseJI GATNNNNATC 1 cut(s) 315
BseLI CCNNNNNNNGG 1 cut(s) 57
BseMI GCAATG 1 cut(s) 341
BseMII CTCAG 3 cut(s) 106, 166, 341
BseNI ACTGG 1 cut(s) 258
BseRI GAGGAG 4 cut(s) 119, 160, 279, 282
BseXI GCAGC 1 cut(s) 391
Bsh1236I CGCG 1 cut(s) 281
BshFI GGCC 2 cut(s) 50, 200
BshTI ACCGGT 1 cut(s) 424
BsiHKAI GWGCWC 1 cut(s) 21
BsiHKCI CYCGRG 1 cut(s) 55
BsiSI CCGG 2 cut(s) 248, 425
BslFI GGGAC 1 cut(s) 18
BslI CCNNNNNNNGG 1 cut(s) 57
BsmAI GTCTC 1 cut(s) 66
BsmFI GGGAC 1 cut(s) 18
BsnI GGCC 2 cut(s) 50, 200
Bso31I GGTCTC 1 cut(s) 66
BsoBI CYCGRG 1 cut(s) 55
Bsp1286I GDGCHC 1 cut(s) 21
Bsp1407I TGTACA 1 cut(s) 126
Bsp143I GATC 4 cut(s) 240, 310, 316, 328
Bsp1720I GCTNAGC 1 cut(s) 350
BspANI GGCC 2 cut(s) 50, 200
BspCNI CTCAG 3 cut(s) 105, 165, 342
BspFNI CGCG 1 cut(s) 281
BspHI TCATGA 1 cut(s) 319
BspPI GGATC 1 cut(s) 248
BspQI GCTCTTC 1 cut(s) 441
BspTNI GGTCTC 1 cut(s) 66
BsrDI GCAATG 1 cut(s) 341
BsrFI RCCGGY 1 cut(s) 424
BsrGI TGTACA 1 cut(s) 126
BsrI ACTGG 1 cut(s) 258
BssAI RCCGGY 1 cut(s) 424
BssMI GATC 4 cut(s) 240, 310, 316, 328
Bst4CI ACNGT 1 cut(s) 234
Bst6I CTCTTC 1 cut(s) 441
BstAUI TGTACA 1 cut(s) 126
BstDEI CTNAG 3 cut(s) 92, 152, 350
BstFNI CGCG 1 cut(s) 281
BstH2I RGCGCY 1 cut(s) 99
BstHHI GCGC 2 cut(s) 98, 281
BstKTI GATC 4 cut(s) 243, 313, 319, 331
BstMAI GTCTC 1 cut(s) 66
BstMBI GATC 4 cut(s) 240, 310, 316, 328
BstMWI GCNNNNNNNGC 3 cut(s) 95, 179, 206
BstUI CGCG 1 cut(s) 281
BstV1I GCAGC 1 cut(s) 391
BstX2I RGATCY 1 cut(s) 240
BstYI RGATCY 1 cut(s) 240
BsuRI GGCC 2 cut(s) 50, 200
BtsIMutI CAGTG 1 cut(s) 265
CciI TCATGA 1 cut(s) 319
CfoI GCGC 2 cut(s) 98, 281
Cfr10I RCCGGY 1 cut(s) 424
Cfr13I GGNCC 1 cut(s) 338
Csp6I GTAC 2 cut(s) 127, 394
CspAI ACCGGT 1 cut(s) 424
CviAII CATG 5 cut(s) 22, 133, 178, 193, 320
CviJI RGCY 8 cut(s) 19, 50, 151, 173, 200, 349, 354, 404
CviKI_1 RGCY 8 cut(s) 19, 50, 151, 173, 200, 349, 354, 404
CviQI GTAC 2 cut(s) 127, 394
DdeI CTNAG 3 cut(s) 92, 152, 350
DpnI GATC 4 cut(s) 242, 312, 318, 330
DpnII GATC 4 cut(s) 240, 310, 316, 328
EaeI YGGCCR 2 cut(s) 48, 198
Eam1104I CTCTTC 1 cut(s) 441
EarI CTCTTC 1 cut(s) 441
EciI GGCGGA 5 cut(s) 29, 76, 196, 243, 398
Ecl136II GAGCTC 1 cut(s) 19
Eco24I GRGCYC 1 cut(s) 21
Eco31I GGTCTC 1 cut(s) 66
Eco47I GGWCC 1 cut(s) 338
Eco53kI GAGCTC 1 cut(s) 19
Eco88I CYCGRG 1 cut(s) 55
EcoICRI GAGCTC 1 cut(s) 19
EcoT38I GRGCYC 1 cut(s) 21
FaeI CATG 5 cut(s) 25, 136, 181, 196, 323
FaiI YATR 7 cut(s) 23, 134, 161, 179, 194, 221, 321
FalI AAGNNNNNCTT 2 cut(s) 288, 320
FaqI GGGAC 1 cut(s) 18
FatI CATG 5 cut(s) 21, 132, 177, 192, 319
FbaI TGATCA 2 cut(s) 310, 328
Fnu4HI GCNGC 5 cut(s) 12, 171, 198, 347, 405
FriOI GRGCYC 1 cut(s) 21
Fsp4HI GCNGC 5 cut(s) 12, 171, 198, 347, 405
FspBI CTAG 1 cut(s) 462
GlaI GCGC 2 cut(s) 97, 280
GluI GCNGC 5 cut(s) 12, 171, 198, 347, 405
GsuI CTGGAG 1 cut(s) 47
HaeII RGCGCY 1 cut(s) 99
HaeIII GGCC 2 cut(s) 50, 200
HapII CCGG 2 cut(s) 248, 425
HhaI GCGC 2 cut(s) 98, 281
Hin1II CATG 5 cut(s) 25, 136, 181, 196, 323
Hin6I GCGC 2 cut(s) 96, 279
HinP1I GCGC 2 cut(s) 96, 279
HpaII CCGG 2 cut(s) 248, 425
HphI GGTGA 2 cut(s) 320, 338
Hpy166II GTNNAC 1 cut(s) 127
Hpy188III TCNNGA 3 cut(s) 314, 320, 462
Hpy8I GTNNAC 1 cut(s) 127
Hpy99I CGWCG 1 cut(s) 104
HpyAV CCTTC 2 cut(s) 52, 317
HpyCH4III ACNGT 1 cut(s) 234
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 1 cut(s) 376
HpyF10VI GCNNNNNNNGC 3 cut(s) 95, 179, 206
HpyF3I CTNAG 3 cut(s) 92, 152, 350
HpySE526I ACGT 1 cut(s) 123
Hsp92II CATG 5 cut(s) 25, 136, 181, 196, 323
HspAI GCGC 2 cut(s) 96, 279
Ksp22I TGATCA 2 cut(s) 310, 328
Kzo9I GATC 4 cut(s) 240, 310, 316, 328
LguI GCTCTTC 1 cut(s) 441
LmnI GCTCC 1 cut(s) 16
LpnPI CCDG 5 cut(s) 11, 210, 261, 271, 438
Lsp1109I GCAGC 1 cut(s) 391
MaeI CTAG 1 cut(s) 462
MaeII ACGT 1 cut(s) 123
MaeIII GTNAC 1 cut(s) 163
MalI GATC 4 cut(s) 242, 312, 318, 330
MboI GATC 4 cut(s) 240, 310, 316, 328
MboII GAAGA 2 cut(s) 181, 458
MflI RGATCY 1 cut(s) 240
MhlI GDGCHC 1 cut(s) 21
MlsI TGGCCA 1 cut(s) 50
MluNI TGGCCA 1 cut(s) 50
MmeI TCCRAC 2 cut(s) 90, 411
Mox20I TGGCCA 1 cut(s) 50
MscI TGGCCA 1 cut(s) 50
MseI TTAA 1 cut(s) 6
MslI CAYNNNNRTG 2 cut(s) 182, 324
Msp20I TGGCCA 1 cut(s) 50
MspI CCGG 2 cut(s) 248, 425
MvnI CGCG 1 cut(s) 281
MwoI GCNNNNNNNGC 3 cut(s) 95, 179, 206
NdeII GATC 4 cut(s) 240, 310, 316, 328
NlaIII CATG 5 cut(s) 25, 136, 181, 196, 323
NmuCI GTSAC 1 cut(s) 163
PaeR7I CTCGAG 1 cut(s) 55
PagI TCATGA 1 cut(s) 319
PciSI GCTCTTC 1 cut(s) 441
PinAI ACCGGT 1 cut(s) 424
PkrI GCNGC 5 cut(s) 13, 172, 199, 348, 406
Psp124BI GAGCTC 1 cut(s) 21
PspPI GGNCC 1 cut(s) 338
PspXI VCTCGAGB 1 cut(s) 55
PsuI RGATCY 1 cut(s) 240
RsaI GTAC 2 cut(s) 128, 395
RsaNI GTAC 2 cut(s) 127, 394
RseI CAYNNNNRTG 2 cut(s) 182, 324
SacI GAGCTC 1 cut(s) 21
SapI GCTCTTC 1 cut(s) 441
SaqAI TTAA 1 cut(s) 6
SatI GCNGC 5 cut(s) 12, 171, 198, 347, 405
Sau3AI GATC 4 cut(s) 240, 310, 316, 328
Sau96I GGNCC 1 cut(s) 338
SduI GDGCHC 1 cut(s) 21
Sfr274I CTCGAG 1 cut(s) 55
SinI GGWCC 1 cut(s) 338
SlaI CTCGAG 1 cut(s) 55
SmiMI CAYNNNNRTG 2 cut(s) 182, 324
SmlI CTYRAG 1 cut(s) 55
SmoI CTYRAG 1 cut(s) 55
SspMI CTAG 1 cut(s) 462
SstI GAGCTC 1 cut(s) 21
TaaI ACNGT 1 cut(s) 234
TaiI ACGT 1 cut(s) 126
TaqI TCGA 1 cut(s) 56
TatI WGTACW 2 cut(s) 126, 393
TauI GCSGC 4 cut(s) 14, 173, 200, 349
Tru1I TTAA 1 cut(s) 6
Tru9I TTAA 1 cut(s) 6
TscAI CASTG 1 cut(s) 265
TseFI GTSAC 1 cut(s) 163
TseI GCWGC 1 cut(s) 404
Tsp45I GTSAC 1 cut(s) 163
TspDTI ATGAA 2 cut(s) 181, 336
TspGWI ACGGA 1 cut(s) 375
TspRI CASTG 1 cut(s) 265
VpaK11BI GGWCC 1 cut(s) 338
XbaI TCTAGA 1 cut(s) 461
XcmI CCANNNNNNNNNTGG 1 cut(s) 43
XhoI CTCGAG 1 cut(s) 55
XspI CTAG 1 cut(s) 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.