AT5G17350

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003619
Physical Location & Seq
Forward (+)
5718498 .. 5719608
1111 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G17350.1

Sequence Viewer

Length: 552 bp
ATGGGAAACTATGTTTCTTCTGCACTAAGCAAAACGTCTTCATCATCATCATCGGCGGCGAAAGTGATTCTCCCCGACGGTGGAGTACGTAACATTCACGCGCCGATGAAAGCTGCCGAGCTTATGATGGAGATTCCAAGCTTCTTCCTCGTCGACGCAAAGTCTTTAAAGATCGGTCGGAAGTTTTGTCCGTTAGCCGCCGATGACGACCTTCAAATCAAAGGCTGCCACGTGTACGTAGCTTTCCCTATGACGCGTGCCACGTCAGCTGCTAATGCTTCGGATTTGGCTCGGTTATTCGTAGCTGCTAAGAAACAGCGCCGCCACCGCGTGGGAAGTGATCATTCTTCCGCCGCCGTGAAACACTGTCATAATAACGGAAGGGTTTCGCCGGACGGGGAAGGAGATGACGTCAAGGTGATTTCAGCGGGATCAAAGCTGAACCTAGAAGATATCGAAGAGTTTTCGGCGGCGGAGTTTATGCATAGGATCTCAGTTTCGAAATCTAAGAAACCAAAATTAGAAACTATAGTTGAAGAATCTTTGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

183

Amino Acids

19.66

Weight (kDa)

8.97

Isoelectric Point (pI)

43.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 179 6.3e-36 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 414
AccB7I CCANNNNNTGG 1 cut(s) 331
AccI GTMKAC 1 cut(s) 153
AccII CGCG 3 cut(s) 101, 256, 330
AciI CCGC 9 cut(s) 56, 198, 322, 328, 351, 354, 428, 470, 473
AclWI GGATC 2 cut(s) 439, 497
AcvI CACGTG 1 cut(s) 232
AcyI GRCGYC 1 cut(s) 411
AdeI CACNNNGTG 1 cut(s) 331
AfaI GTAC 2 cut(s) 87, 236
AfiI CCNNNNNNNGG 2 cut(s) 80, 331
AflIII ACRYGT 2 cut(s) 231, 254
AgsI TTSAA 2 cut(s) 215, 536
AhdI GACNNNNNGTC 1 cut(s) 160
AjiI CACGTC 1 cut(s) 264
AluBI AGCT 7 cut(s) 113, 121, 141, 242, 269, 305, 439
AluI AGCT 7 cut(s) 113, 121, 141, 242, 269, 305, 439
AlwI GGATC 2 cut(s) 439, 497
ApeKI GCWGC 4 cut(s) 113, 225, 269, 305
Asp700I GAANNNNTTC 1 cut(s) 385
AspLEI GCGC 2 cut(s) 103, 321
AsuHPI GGTGA 1 cut(s) 430
AsuII TTCGAA 1 cut(s) 500
BbrPI CACGTG 1 cut(s) 232
BbsI GAAGAC 1 cut(s) 30
BbvI GCAGC 4 cut(s) 100, 212, 256, 292
BccI CCATC 1 cut(s) 121
BceAI ACGGC 1 cut(s) 341
BclI TGATCA 1 cut(s) 340
BfaI CTAG 1 cut(s) 446
BfmI CTRYAG 1 cut(s) 528
BfoI RGCGCY 1 cut(s) 322
BisI GCNGC 9 cut(s) 57, 114, 198, 226, 270, 306, 322, 354, 471
BlsI GCNGC 9 cut(s) 58, 115, 199, 227, 271, 307, 323, 355, 472
BmeRI GACNNNNNGTC 1 cut(s) 160
BmgBI CACGTC 1 cut(s) 264
BpiI GAAGAC 1 cut(s) 30
Bpu14I TTCGAA 1 cut(s) 500
BsaAI YACGTR 3 cut(s) 89, 232, 238
BsaHI GRCGYC 1 cut(s) 411
BsaXI ACNNNNNCTCC 2 cut(s) 396, 426
Bsc4I CCNNNNNNNGG 2 cut(s) 80, 331
BseLI CCNNNNNNNGG 2 cut(s) 80, 331
BseMII CTCAG 1 cut(s) 507
BseXI GCAGC 4 cut(s) 100, 212, 256, 292
BsgI GTGCAG 1 cut(s) 6
Bsh1236I CGCG 3 cut(s) 101, 256, 330
Bsh1285I CGRYCG 1 cut(s) 178
BsiEI CGRYCG 1 cut(s) 178
BsiSI CCGG 1 cut(s) 392
BslI CCNNNNNNNGG 2 cut(s) 80, 331
Bsp119I TTCGAA 1 cut(s) 500
Bsp143I GATC 4 cut(s) 171, 340, 431, 489
BspACI CCGC 9 cut(s) 56, 198, 322, 328, 351, 354, 428, 470, 473
BspCNI CTCAG 1 cut(s) 506
BspFNI CGCG 3 cut(s) 101, 256, 330
BspPI GGATC 2 cut(s) 439, 497
BspT104I TTCGAA 1 cut(s) 500
BssMI GATC 4 cut(s) 171, 340, 431, 489
BssNI GRCGYC 1 cut(s) 411
Bst4CI ACNGT 2 cut(s) 80, 368
Bst6I CTCTTC 1 cut(s) 453
BstACI GRCGYC 1 cut(s) 411
BstBAI YACGTR 3 cut(s) 89, 232, 238
BstBI TTCGAA 1 cut(s) 500
BstC8I GCNNGC 1 cut(s) 258
BstDEI CTNAG 4 cut(s) 26, 309, 493, 507
BstFNI CGCG 3 cut(s) 101, 256, 330
BstH2I RGCGCY 1 cut(s) 322
BstHHI GCGC 2 cut(s) 103, 321
BstKTI GATC 4 cut(s) 174, 343, 434, 492
BstMBI GATC 4 cut(s) 171, 340, 431, 489
BstMCI CGRYCG 1 cut(s) 178
BstMWI GCNNNNNNNGC 3 cut(s) 266, 275, 327
BstSFI CTRYAG 1 cut(s) 528
BstSNI TACGTA 2 cut(s) 89, 238
BstUI CGCG 3 cut(s) 101, 256, 330
BstV1I GCAGC 4 cut(s) 100, 212, 256, 292
BstV2I GAAGAC 1 cut(s) 30
BstX2I RGATCY 1 cut(s) 489
BstYI RGATCY 1 cut(s) 489
BtrI CACGTC 1 cut(s) 264
BtsIMutI CAGTG 1 cut(s) 364
Cac8I GCNNGC 1 cut(s) 258
CfoI GCGC 2 cut(s) 103, 321
CseI GACGC 2 cut(s) 164, 262
Csp6I GTAC 2 cut(s) 86, 235
CviQI GTAC 2 cut(s) 86, 235
DdeI CTNAG 4 cut(s) 26, 309, 493, 507
DpnI GATC 4 cut(s) 173, 342, 433, 491
DpnII GATC 4 cut(s) 171, 340, 431, 489
DraI TTTAAA 1 cut(s) 168
DraIII CACNNNGTG 1 cut(s) 331
DriI GACNNNNNGTC 1 cut(s) 160
Eam1104I CTCTTC 1 cut(s) 453
Eam1105I GACNNNNNGTC 1 cut(s) 160
EarI CTCTTC 1 cut(s) 453
EciI GGCGGA 2 cut(s) 340, 488
Eco105I TACGTA 2 cut(s) 89, 238
Eco32I GATATC 1 cut(s) 454
Eco72I CACGTG 1 cut(s) 232
EcoRV GATATC 1 cut(s) 454
EcoT22I ATGCAT 1 cut(s) 486
FaiI YATR 7 cut(s) 12, 125, 251, 372, 482, 486, 530
FauI CCCGC 1 cut(s) 421
FbaI TGATCA 1 cut(s) 340
FblI GTMKAC 1 cut(s) 153
Fnu4HI GCNGC 9 cut(s) 57, 114, 198, 226, 270, 306, 322, 354, 471
Fsp4HI GCNGC 9 cut(s) 57, 114, 198, 226, 270, 306, 322, 354, 471
FspBI CTAG 1 cut(s) 446
GlaI GCGC 2 cut(s) 102, 320
GluI GCNGC 9 cut(s) 57, 114, 198, 226, 270, 306, 322, 354, 471
HaeII RGCGCY 1 cut(s) 322
HapII CCGG 1 cut(s) 392
HgaI GACGC 2 cut(s) 164, 262
HhaI GCGC 2 cut(s) 103, 321
Hin1I GRCGYC 1 cut(s) 411
Hin6I GCGC 2 cut(s) 101, 319
HinP1I GCGC 2 cut(s) 101, 319
HincII GTYRAC 1 cut(s) 154
HindII GTYRAC 1 cut(s) 154
HindIII AAGCTT 1 cut(s) 139
HinfI GANTC 3 cut(s) 67, 133, 539
HpaII CCGG 1 cut(s) 392
HphI GGTGA 1 cut(s) 430
Hpy166II GTNNAC 2 cut(s) 154, 235
Hpy188I TCNGA 2 cut(s) 180, 283
Hpy8I GTNNAC 2 cut(s) 154, 235
Hpy99I CGWCG 3 cut(s) 80, 155, 158
HpyAV CCTTC 3 cut(s) 221, 375, 395
HpyCH4III ACNGT 2 cut(s) 80, 368
HpyCH4IV ACGT 6 cut(s) 35, 88, 231, 237, 263, 411
HpyCH4V TGCA 2 cut(s) 23, 484
HpyF10VI GCNNNNNNNGC 3 cut(s) 266, 275, 327
HpyF3I CTNAG 4 cut(s) 26, 309, 493, 507
HpySE526I ACGT 6 cut(s) 35, 88, 231, 237, 263, 411
Hsp92I GRCGYC 1 cut(s) 411
HspAI GCGC 2 cut(s) 101, 319
Ksp22I TGATCA 1 cut(s) 340
Kzo9I GATC 4 cut(s) 171, 340, 431, 489
LpnPI CCDG 1 cut(s) 405
Lsp1109I GCAGC 4 cut(s) 100, 212, 256, 292
MaeI CTAG 1 cut(s) 446
MaeII ACGT 6 cut(s) 35, 88, 231, 237, 263, 411
MaeIII GTNAC 1 cut(s) 89
MalI GATC 4 cut(s) 173, 342, 433, 491
MboI GATC 4 cut(s) 171, 340, 431, 489
MboII GAAGA 7 cut(s) 9, 30, 136, 339, 461, 470, 548
MflI RGATCY 1 cut(s) 489
MluCI AATT 1 cut(s) 518
MluI ACGCGT 1 cut(s) 254
MmeI TCCRAC 1 cut(s) 158
MnlI CCTC 1 cut(s) 158
Mph1103I ATGCAT 1 cut(s) 486
MroXI GAANNNNTTC 1 cut(s) 385
MseI TTAA 2 cut(s) 167, 550
MspA1I CMGCKG 2 cut(s) 269, 428
MspI CCGG 1 cut(s) 392
MvnI CGCG 3 cut(s) 101, 256, 330
MwoI GCNNNNNNNGC 3 cut(s) 266, 275, 327
NdeII GATC 4 cut(s) 171, 340, 431, 489
NmeAIII GCCGAG 1 cut(s) 142
NsiI ATGCAT 1 cut(s) 486
NspV TTCGAA 1 cut(s) 500
PcsI WCGNNNNNNNCGW 1 cut(s) 260
PdmI GAANNNNTTC 1 cut(s) 385
PfeI GAWTC 3 cut(s) 67, 133, 539
PflMI CCANNNNNTGG 1 cut(s) 331
PkrI GCNGC 9 cut(s) 58, 115, 199, 227, 271, 307, 323, 355, 472
PmaCI CACGTG 1 cut(s) 232
PmlI CACGTG 1 cut(s) 232
Ppu21I YACGTR 3 cut(s) 89, 232, 238
PspCI CACGTG 1 cut(s) 232
PsuI RGATCY 1 cut(s) 489
PvuII CAGCTG 1 cut(s) 269
RsaI GTAC 2 cut(s) 87, 236
RsaNI GTAC 2 cut(s) 86, 235
SalI GTCGAC 1 cut(s) 152
SaqAI TTAA 2 cut(s) 167, 550
SatI GCNGC 9 cut(s) 57, 114, 198, 226, 270, 306, 322, 354, 471
Sau3AI GATC 4 cut(s) 171, 340, 431, 489
SfcI CTRYAG 1 cut(s) 528
SfuI TTCGAA 1 cut(s) 500
SgrDI CGTCGACG 1 cut(s) 152
SnaBI TACGTA 2 cut(s) 89, 238
Sse9I AATT 1 cut(s) 518
SsiI CCGC 9 cut(s) 56, 198, 322, 328, 351, 354, 428, 470, 473
SspMI CTAG 1 cut(s) 446
TaaI ACNGT 2 cut(s) 80, 368
TaiI ACGT 6 cut(s) 38, 91, 234, 240, 266, 414
TaqI TCGA 3 cut(s) 153, 456, 500
TaqII GACCGA 1 cut(s) 164
TasI AATT 1 cut(s) 518
TauI GCSGC 5 cut(s) 59, 200, 324, 356, 473
TfiI GAWTC 3 cut(s) 67, 133, 539
Tru1I TTAA 2 cut(s) 167, 550
Tru9I TTAA 2 cut(s) 167, 550
TscAI CASTG 1 cut(s) 371
TseI GCWGC 4 cut(s) 113, 225, 269, 305
TspDTI ATGAA 2 cut(s) 30, 122
TspGWI ACGGA 2 cut(s) 180, 393
TspRI CASTG 1 cut(s) 371
Van91I CCANNNNNTGG 1 cut(s) 331
XmiI GTMKAC 1 cut(s) 153
XmnI GAANNNNTTC 1 cut(s) 385
XspI CTAG 1 cut(s) 446
ZraI GACGTC 1 cut(s) 412
Zsp2I ATGCAT 1 cut(s) 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.