RLG00000006734

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0003619
Physical Location & Seq
Forward (+)
10046361 .. 10046909
549 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006734

Sequence Viewer

Length: 549 bp
ATGGGGAACTACATCTCATGCACTCTAGCCACGCCTCTCATCAAGAGCACCAAGGCGGCGCGTGTGGTCTTTCCTACAGGCGAAGTAAGGCAGTTCCGTGAACCGATCAAGGCCGCCGAGCTCATGCTGGAGTCGCCGAACACGTTCCTAGCCAACTCCAAGTCTCTCCACATCGGCCGCCGATTCTCCGCCCTCGGCGCCGACGAGGAGCTCGAGTTCGGCAACGTCTACATCATGTTCCCGATGAGGCGGCTCAGCTCCATCGTCACCGCCGCCGACATGACCATCTTTTTCATTGCGGCCAACTCCGCCGCCAAGCGGATTTCAGGCGGGAAGGTCCGGATCTTGCCTGAGGCTCAAGCAGCCCAAAACGACGAGGAGGAGGAGAAAAGTCAAAGCGGTGACGGAGAAGGTCAAGGTCCGGCGAGGCTGAGCCTGGACGGAGTTGAGGGTTTGCAAGTGGCGGAGTTCCAGTATAGATTAGCTGCGTGTAGGTCAAGGAAGCCAATGTTGGAGACGATCAAAGAGGAGCCGATTTTTTCAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

183

Amino Acids

20.0

Weight (kDa)

6.63

Isoelectric Point (pI)

48.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 176 1e-38 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 197
AccI GTMKAC 1 cut(s) 228
AccII CGCG 1 cut(s) 61
AccIII TCCGGA 1 cut(s) 339
AclWI GGATC 1 cut(s) 350
AcoI YGGCCR 2 cut(s) 175, 300
AcyI GRCGYC 1 cut(s) 198
AfiI CCNNNNNNNGG 1 cut(s) 318
AflIII ACRYGT 1 cut(s) 141
AgsI TTSAA 1 cut(s) 543
AjnI CCWGG 1 cut(s) 435
AjuI GAANNNNNNNTTGG 2 cut(s) 494, 526
AloI GAACNNNNNNTCC 2 cut(s) 200, 232
AluBI AGCT 4 cut(s) 121, 211, 258, 485
AluI AGCT 4 cut(s) 121, 211, 258, 485
Alw21I GWGCWC 3 cut(s) 50, 123, 213
Alw26I GTCTC 2 cut(s) 168, 509
AlwI GGATC 1 cut(s) 350
Ama87I CYCGRG 1 cut(s) 212
Aor13HI TCCGGA 1 cut(s) 339
AoxI GGCC 3 cut(s) 111, 175, 300
ApeKI GCWGC 2 cut(s) 362, 485
Asp700I GAANNNNTTC 1 cut(s) 143
AspLEI GCGC 2 cut(s) 61, 200
AspS9I GGNCC 2 cut(s) 337, 419
AsuHPI GGTGA 2 cut(s) 259, 413
AvaI CYCGRG 1 cut(s) 212
AvaII GGWCC 2 cut(s) 337, 419
AxyI CCTNAGG 1 cut(s) 351
BanI GGYRCC 1 cut(s) 197
BanII GRGCYC 2 cut(s) 123, 213
Bbv12I GWGCWC 3 cut(s) 50, 123, 213
BbvI GCAGC 2 cut(s) 374, 472
BccI CCATC 2 cut(s) 269, 293
BciT130I CCWGG 1 cut(s) 437
BcoDI GTCTC 2 cut(s) 168, 509
BfaI CTAG 2 cut(s) 26, 149
BfmI CTRYAG 1 cut(s) 75
BfoI RGCGCY 1 cut(s) 201
BisI GCNGC 9 cut(s) 57, 114, 178, 251, 273, 300, 312, 363, 486
BlpI GCTNAGC 2 cut(s) 254, 431
BlsI GCNGC 9 cut(s) 58, 115, 179, 252, 274, 301, 313, 364, 487
Bme1390I CCNGG 1 cut(s) 437
Bme18I GGWCC 2 cut(s) 337, 419
BmeT110I CYCGRG 1 cut(s) 212
BmgT120I GGNCC 2 cut(s) 337, 419
BmiI GGNNCC 2 cut(s) 199, 531
BmrFI CCNGG 1 cut(s) 437
BpmI CTGGAG 1 cut(s) 149
Bpu1102I GCTNAGC 2 cut(s) 254, 431
BpuEI CTTGAG 1 cut(s) 342
BsaHI GRCGYC 1 cut(s) 198
BsaJI CCNNGG 2 cut(s) 51, 193
BsaWI WCCGGW 1 cut(s) 339
BsaXI ACNNNNNCTCC 4 cut(s) 200, 230, 458, 488
Bsc4I CCNNNNNNNGG 1 cut(s) 318
Bse1I ACTGG 1 cut(s) 472
Bse21I CCTNAGG 1 cut(s) 351
Bse3DI GCAATG 1 cut(s) 294
BseAI TCCGGA 1 cut(s) 339
BseBI CCWGG 1 cut(s) 437
BseDI CCNNGG 2 cut(s) 51, 193
BseLI CCNNNNNNNGG 1 cut(s) 318
BseMI GCAATG 1 cut(s) 294
BseMII CTCAG 3 cut(s) 268, 342, 422
BseNI ACTGG 1 cut(s) 472
BseRI GAGGAG 5 cut(s) 221, 392, 395, 398, 542
BseX3I CGGCCG 1 cut(s) 175
BseXI GCAGC 2 cut(s) 374, 472
Bsh1236I CGCG 1 cut(s) 61
Bsh1285I CGRYCG 1 cut(s) 178
BshFI GGCC 3 cut(s) 113, 177, 302
BshNI GGYRCC 1 cut(s) 197
BsiEI CGRYCG 1 cut(s) 178
BsiHKAI GWGCWC 3 cut(s) 50, 123, 213
BsiHKCI CYCGRG 1 cut(s) 212
BsiSI CCGG 2 cut(s) 340, 422
BslI CCNNNNNNNGG 1 cut(s) 318
BsmAI GTCTC 2 cut(s) 168, 509
BsmBI CGTCTC 1 cut(s) 509
BsnI GGCC 3 cut(s) 113, 177, 302
BsoBI CYCGRG 1 cut(s) 212
Bsp1286I GDGCHC 3 cut(s) 50, 123, 213
Bsp13I TCCGGA 1 cut(s) 339
Bsp143I GATC 3 cut(s) 105, 342, 519
Bsp1720I GCTNAGC 2 cut(s) 254, 431
BspANI GGCC 3 cut(s) 113, 177, 302
BspCNI CTCAG 3 cut(s) 267, 343, 423
BspEI TCCGGA 1 cut(s) 339
BspFNI CGCG 1 cut(s) 61
BspLI GGNNCC 2 cut(s) 199, 531
BspPI GGATC 1 cut(s) 350
BspT107I GGYRCC 1 cut(s) 197
BsrDI GCAATG 1 cut(s) 294
BsrI ACTGG 1 cut(s) 472
BssECI CCNNGG 2 cut(s) 51, 193
BssMI GATC 3 cut(s) 105, 342, 519
BssNI GRCGYC 1 cut(s) 198
BssT1I CCWWGG 1 cut(s) 51
Bst2UI CCWGG 1 cut(s) 437
BstACI GRCGYC 1 cut(s) 198
BstDEI CTNAG 3 cut(s) 254, 351, 431
BstFNI CGCG 1 cut(s) 61
BstH2I RGCGCY 1 cut(s) 201
BstHHI GCGC 2 cut(s) 61, 200
BstKTI GATC 3 cut(s) 108, 345, 522
BstMAI GTCTC 2 cut(s) 168, 509
BstMBI GATC 3 cut(s) 105, 342, 519
BstMCI CGRYCG 1 cut(s) 178
BstMWI GCNNNNNNNGC 4 cut(s) 133, 197, 308, 362
BstNI CCWGG 1 cut(s) 437
BstSCI CCNGG 1 cut(s) 435
BstSFI CTRYAG 1 cut(s) 75
BstUI CGCG 1 cut(s) 61
BstV1I GCAGC 2 cut(s) 374, 472
BstX2I RGATCY 1 cut(s) 342
BstYI RGATCY 1 cut(s) 342
BstZI CGGCCG 1 cut(s) 175
Bsu36I CCTNAGG 1 cut(s) 351
BsuRI GGCC 3 cut(s) 113, 177, 302
CfoI GCGC 2 cut(s) 61, 200
Cfr13I GGNCC 2 cut(s) 337, 419
CviAII CATG 4 cut(s) 18, 124, 235, 280
DdeI CTNAG 3 cut(s) 254, 351, 431
DinI GGCGCC 1 cut(s) 199
DpnI GATC 3 cut(s) 107, 344, 521
DpnII GATC 3 cut(s) 105, 342, 519
EaeI YGGCCR 2 cut(s) 175, 300
EagI CGGCCG 1 cut(s) 175
EciI GGCGGA 3 cut(s) 178, 298, 479
Ecl136II GAGCTC 2 cut(s) 121, 211
EclXI CGGCCG 1 cut(s) 175
Eco130I CCWWGG 1 cut(s) 51
Eco24I GRGCYC 2 cut(s) 123, 213
Eco47I GGWCC 2 cut(s) 337, 419
Eco52I CGGCCG 1 cut(s) 175
Eco53kI GAGCTC 2 cut(s) 121, 211
Eco81I CCTNAGG 1 cut(s) 351
Eco88I CYCGRG 1 cut(s) 212
EcoICRI GAGCTC 2 cut(s) 121, 211
EcoRII CCWGG 1 cut(s) 435
EcoT14I CCWWGG 1 cut(s) 51
EcoT38I GRGCYC 2 cut(s) 123, 213
EgeI GGCGCC 1 cut(s) 199
EheI GGCGCC 1 cut(s) 199
ErhI CCWWGG 1 cut(s) 51
Esp3I CGTCTC 1 cut(s) 509
FaeI CATG 4 cut(s) 21, 127, 238, 283
FaiI YATR 5 cut(s) 19, 125, 236, 281, 477
FatI CATG 4 cut(s) 17, 123, 234, 279
FauI CCCGC 1 cut(s) 323
FblI GTMKAC 1 cut(s) 228
Fnu4HI GCNGC 9 cut(s) 57, 114, 178, 251, 273, 300, 312, 363, 486
FriOI GRGCYC 2 cut(s) 123, 213
Fsp4HI GCNGC 9 cut(s) 57, 114, 178, 251, 273, 300, 312, 363, 486
FspBI CTAG 2 cut(s) 26, 149
GlaI GCGC 2 cut(s) 60, 199
GluI GCNGC 9 cut(s) 57, 114, 178, 251, 273, 300, 312, 363, 486
GsuI CTGGAG 1 cut(s) 149
HaeII RGCGCY 1 cut(s) 201
HaeIII GGCC 3 cut(s) 113, 177, 302
HapII CCGG 2 cut(s) 340, 422
HhaI GCGC 2 cut(s) 61, 200
Hin1I GRCGYC 1 cut(s) 198
Hin1II CATG 4 cut(s) 21, 127, 238, 283
Hin6I GCGC 2 cut(s) 59, 198
HinP1I GCGC 2 cut(s) 59, 198
HinfI GANTC 2 cut(s) 131, 183
HpaII CCGG 2 cut(s) 340, 422
HphI GGTGA 2 cut(s) 259, 413
Hpy166II GTNNAC 2 cut(s) 101, 229
Hpy188III TCNNGA 4 cut(s) 43, 241, 340, 543
Hpy8I GTNNAC 2 cut(s) 101, 229
Hpy99I CGWCG 2 cut(s) 206, 377
HpyAV CCTTC 2 cut(s) 328, 404
HpyCH4IV ACGT 2 cut(s) 143, 225
HpyCH4V TGCA 2 cut(s) 21, 457
HpyF10VI GCNNNNNNNGC 4 cut(s) 133, 197, 308, 362
HpyF3I CTNAG 3 cut(s) 254, 351, 431
HpySE526I ACGT 2 cut(s) 143, 225
Hsp92I GRCGYC 1 cut(s) 198
Hsp92II CATG 4 cut(s) 21, 127, 238, 283
HspAI GCGC 2 cut(s) 59, 198
KasI GGCGCC 1 cut(s) 197
Kpn2I TCCGGA 1 cut(s) 339
Kzo9I GATC 3 cut(s) 105, 342, 519
LmnI GCTCC 3 cut(s) 208, 263, 529
LpnPI CCDG 9 cut(s) 63, 113, 312, 353, 363, 422, 435, 449, 485
Lsp1109I GCAGC 2 cut(s) 374, 472
MaeI CTAG 2 cut(s) 26, 149
MaeII ACGT 2 cut(s) 143, 225
MaeIII GTNAC 2 cut(s) 265, 401
MalI GATC 3 cut(s) 107, 344, 521
MboI GATC 3 cut(s) 105, 342, 519
MflI RGATCY 1 cut(s) 342
MhlI GDGCHC 3 cut(s) 50, 123, 213
Mly113I GGCGCC 1 cut(s) 198
MlyI GAGTC 1 cut(s) 140
MmeI TCCRAC 1 cut(s) 492
MroI TCCGGA 1 cut(s) 339
MroXI GAANNNNTTC 1 cut(s) 143
MspI CCGG 2 cut(s) 340, 422
MspR9I CCNGG 1 cut(s) 437
MvaI CCWGG 1 cut(s) 437
MvnI CGCG 1 cut(s) 61
MwoI GCNNNNNNNGC 4 cut(s) 133, 197, 308, 362
NarI GGCGCC 1 cut(s) 198
NdeII GATC 3 cut(s) 105, 342, 519
NlaIII CATG 4 cut(s) 21, 127, 238, 283
NlaIV GGNNCC 2 cut(s) 199, 531
NmeAIII GCCGAG 2 cut(s) 142, 174
NmuCI GTSAC 2 cut(s) 265, 401
PaeR7I CTCGAG 1 cut(s) 212
PcsI WCGNNNNNNNCGW 3 cut(s) 140, 201, 210
PdmI GAANNNNTTC 1 cut(s) 143
PfeI GAWTC 1 cut(s) 183
PkrI GCNGC 9 cut(s) 58, 115, 179, 252, 274, 301, 313, 364, 487
PleI GAGTC 1 cut(s) 139
PluTI GGCGCC 1 cut(s) 201
PpsI GAGTC 1 cut(s) 139
Psp124BI GAGCTC 2 cut(s) 123, 213
Psp6I CCWGG 1 cut(s) 435
PspGI CCWGG 1 cut(s) 435
PspN4I GGNNCC 2 cut(s) 199, 531
PspPI GGNCC 2 cut(s) 337, 419
PspXI VCTCGAGB 1 cut(s) 212
PsuI RGATCY 1 cut(s) 342
SacI GAGCTC 2 cut(s) 123, 213
SatI GCNGC 9 cut(s) 57, 114, 178, 251, 273, 300, 312, 363, 486
Sau3AI GATC 3 cut(s) 105, 342, 519
Sau96I GGNCC 2 cut(s) 337, 419
SchI GAGTC 1 cut(s) 140
ScrFI CCNGG 1 cut(s) 437
SduI GDGCHC 3 cut(s) 50, 123, 213
SfcI CTRYAG 1 cut(s) 75
SfoI GGCGCC 1 cut(s) 199
Sfr274I CTCGAG 1 cut(s) 212
SinI GGWCC 2 cut(s) 337, 419
SlaI CTCGAG 1 cut(s) 212
SmlI CTYRAG 2 cut(s) 212, 357
SmoI CTYRAG 2 cut(s) 212, 357
SspDI GGCGCC 1 cut(s) 197
SspMI CTAG 2 cut(s) 26, 149
SstI GAGCTC 2 cut(s) 123, 213
StyD4I CCNGG 1 cut(s) 435
StyI CCWWGG 1 cut(s) 51
TaiI ACGT 2 cut(s) 146, 228
TaqI TCGA 1 cut(s) 213
TauI GCSGC 7 cut(s) 59, 116, 180, 253, 275, 302, 314
TfiI GAWTC 1 cut(s) 183
TseFI GTSAC 2 cut(s) 265, 401
TseI GCWGC 2 cut(s) 362, 485
Tsp45I GTSAC 2 cut(s) 265, 401
TspDTI ATGAA 1 cut(s) 283
TspGWI ACGGA 3 cut(s) 86, 420, 456
VpaK11BI GGWCC 2 cut(s) 337, 419
XhoI CTCGAG 1 cut(s) 212
XmiI GTMKAC 1 cut(s) 228
XmnI GAANNNNTTC 1 cut(s) 143
XspI CTAG 2 cut(s) 26, 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.