RLG00000022695

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0003619
Physical Location & Seq
Reverse (-)
9964643 .. 9966827
2185 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022695

Sequence Viewer

Length: 780 bp
ATGGCTGTCCAACTATTGCTCAACGAACCACCTCATTCGAGATCCACAAGTTTCTCATCTTCCTCATTGTCCAACCCCACCAAGATTGCTAATCGCCCCCATTTTGGTCTCAAGCCATCTATGGTTTTCTCCGTGCTCGCAATTTCAGTCAAGTCGAGATCGTCCCGGCAAGGTGTGATTGCAGTGAAAGCACCCAAACCTAGACGTGCATGGGTTGTCGCCAAGAAGCATCAACGTCTGTCAAGTAATACTGGAGGAGGAAAGCACTGCAAAGTGACCAAAGTAGTGGTTCCAAGCGGTGAAGTTCGAGAGATGAATGAACCGGTAAAAGCCGCGGAGGTGATGCTCGACACCCCAAACTACTTCCTGGTAAACTCCAAGTGTCTCCATATGGGCAGGAGATTTTCTGCTCTTACTGCAGACCAAGACTTGGAGATGGGCAGCGTTTACGTCATGTTTCCCATGAAGAGGCTCAACTCTACAGTCACATCTTCTGACATGGCCGCCTTGTTCCTCACCGCCGTTGCCACTCCCAAGGCCATGCACAAGCCCAAACGGCAGCTTTCCGGTGGCAAGATCAGGGTGCAGCCGGCTGATCATGACGATCACGATCAAACCACCATTAATAAGATGAATTCAGACAAGGGTCCAAAGCTAAATTTGGATGAAATCGAAGAGTTCTTGGCCCCAGAGTTCATGCATCGATTATCCATGAGTAGGTCCAAGAAGCCATTGTTGGAGACCATAACAGAAGAGGCTGTTAGTGTTTGTTCAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

260

Amino Acids

28.62

Weight (kDa)

9.94

Isoelectric Point (pI)

51.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 90 - 251 1.1e-33 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 430
AccII CGCG 1 cut(s) 335
AciI CCGC 5 cut(s) 297, 333, 335, 504, 519
AclWI GGATC 1 cut(s) 36
AcoI YGGCCR 1 cut(s) 501
AcsI RAATTY 2 cut(s) 634, 658
AfiI CCNNNNNNNGG 4 cut(s) 104, 430, 468, 717
AgeI ACCGGT 1 cut(s) 322
AgsI TTSAA 1 cut(s) 774
AjiI CACGTC 1 cut(s) 206
AjnI CCWGG 1 cut(s) 366
AjuI GAANNNNNNNTTGG 2 cut(s) 719, 751
AluBI AGCT 2 cut(s) 562, 655
AluI AGCT 2 cut(s) 562, 655
Alw21I GWGCWC 1 cut(s) 138
Alw26I GTCTC 3 cut(s) 113, 389, 734
AlwI GGATC 1 cut(s) 36
AoxI GGCC 3 cut(s) 501, 537, 684
ApeKI GCWGC 3 cut(s) 441, 559, 586
ApoI RAATTY 2 cut(s) 634, 658
AseI ATTAAT 1 cut(s) 624
AsiGI ACCGGT 1 cut(s) 322
AspS9I GGNCC 3 cut(s) 647, 685, 720
AsuC2I CCSGG 1 cut(s) 166
AsuHPI GGTGA 3 cut(s) 311, 352, 508
AvaII GGWCC 2 cut(s) 647, 720
Bbv12I GWGCWC 1 cut(s) 138
BbvI GCAGC 3 cut(s) 453, 571, 598
BccI CCATC 2 cut(s) 124, 430
BceAI ACGGC 2 cut(s) 506, 572
BciT130I CCWGG 1 cut(s) 368
BclI TGATCA 1 cut(s) 595
BcnI CCSGG 1 cut(s) 166
BcoDI GTCTC 3 cut(s) 113, 389, 734
BfaI CTAG 1 cut(s) 201
BfmI CTRYAG 2 cut(s) 417, 480
BglI GCCNNNNNGGC 1 cut(s) 556
BisI GCNGC 5 cut(s) 333, 442, 504, 560, 587
BlsI GCNGC 5 cut(s) 334, 443, 505, 561, 588
Bme1390I CCNGG 2 cut(s) 166, 368
Bme18I GGWCC 2 cut(s) 647, 720
BmgBI CACGTC 1 cut(s) 206
BmgT120I GGNCC 3 cut(s) 647, 685, 720
BmiI GGNNCC 3 cut(s) 291, 648, 687
BmrFI CCNGG 2 cut(s) 166, 368
BmsI GCATC 3 cut(s) 238, 333, 709
BoxI GACNNNNGTC 1 cut(s) 645
BpmI CTGGAG 1 cut(s) 273
BpuEI CTTGAG 1 cut(s) 95
BpuMI CCSGG 1 cut(s) 166
Bsa29I ATCGAT 1 cut(s) 703
BsaBI GATNNNNATC 1 cut(s) 609
BsaI GGTCTC 2 cut(s) 113, 734
BsaJI CCNNGG 2 cut(s) 333, 534
BsaWI WCCGGW 2 cut(s) 322, 566
Bsc4I CCNNNNNNNGG 4 cut(s) 104, 430, 468, 717
Bse118I RCCGGY 2 cut(s) 322, 589
Bse1I ACTGG 1 cut(s) 256
Bse8I GATNNNNATC 1 cut(s) 609
BseBI CCWGG 1 cut(s) 368
BseCI ATCGAT 1 cut(s) 703
BseDI CCNNGG 2 cut(s) 333, 534
BseGI GGATG 1 cut(s) 670
BseJI GATNNNNATC 1 cut(s) 609
BseLI CCNNNNNNNGG 4 cut(s) 104, 430, 468, 717
BseNI ACTGG 1 cut(s) 256
BseRI GAGGAG 1 cut(s) 270
BseXI GCAGC 3 cut(s) 453, 571, 598
BsgI GTGCAG 1 cut(s) 605
Bsh1236I CGCG 1 cut(s) 335
BshFI GGCC 3 cut(s) 503, 539, 686
BshTI ACCGGT 1 cut(s) 322
BshVI ATCGAT 1 cut(s) 703
BsiHKAI GWGCWC 1 cut(s) 138
BsiSI CCGG 4 cut(s) 166, 323, 567, 590
BslFI GGGAC 1 cut(s) 148
BslI CCNNNNNNNGG 4 cut(s) 104, 430, 468, 717
BsmAI GTCTC 3 cut(s) 113, 389, 734
BsmFI GGGAC 1 cut(s) 148
BsnI GGCC 3 cut(s) 503, 539, 686
Bso31I GGTCTC 2 cut(s) 113, 734
Bsp1286I GDGCHC 1 cut(s) 138
Bsp143I GATC 6 cut(s) 41, 158, 576, 595, 604, 610
BspACI CCGC 5 cut(s) 297, 333, 335, 504, 519
BspANI GGCC 3 cut(s) 503, 539, 686
BspDI ATCGAT 1 cut(s) 703
BspFNI CGCG 1 cut(s) 335
BspHI TCATGA 1 cut(s) 598
BspLI GGNNCC 3 cut(s) 291, 648, 687
BspMAI CTGCAG 1 cut(s) 421
BspPI GGATC 1 cut(s) 36
BspTNI GGTCTC 2 cut(s) 113, 734
BsrFI RCCGGY 2 cut(s) 322, 589
BsrI ACTGG 1 cut(s) 256
BssAI RCCGGY 2 cut(s) 322, 589
BssECI CCNNGG 2 cut(s) 333, 534
BssMI GATC 6 cut(s) 41, 158, 576, 595, 604, 610
BssT1I CCWWGG 1 cut(s) 534
Bst2UI CCWGG 1 cut(s) 368
Bst4CI ACNGT 1 cut(s) 484
Bst6I CTCTTC 3 cut(s) 461, 669, 747
BstC8I GCNNGC 2 cut(s) 138, 591
BstDSI CCRYGG 1 cut(s) 333
BstF5I GGATG 1 cut(s) 670
BstFNI CGCG 1 cut(s) 335
BstKTI GATC 6 cut(s) 44, 161, 579, 598, 607, 613
BstMAI GTCTC 3 cut(s) 113, 389, 734
BstMBI GATC 6 cut(s) 41, 158, 576, 595, 604, 610
BstMWI GCNNNNNNNGC 3 cut(s) 188, 416, 556
BstNI CCWGG 1 cut(s) 368
BstPAI GACNNNNGTC 1 cut(s) 645
BstSCI CCNGG 2 cut(s) 164, 366
BstSFI CTRYAG 2 cut(s) 417, 480
BstUI CGCG 1 cut(s) 335
BstV1I GCAGC 3 cut(s) 453, 571, 598
BstX2I RGATCY 1 cut(s) 41
BstXI CCANNNNNNTGG 1 cut(s) 286
BstYI RGATCY 1 cut(s) 41
Bsu15I ATCGAT 1 cut(s) 703
BsuRI GGCC 3 cut(s) 503, 539, 686
BsuTUI ATCGAT 1 cut(s) 703
BtgI CCRYGG 1 cut(s) 333
BtrI CACGTC 1 cut(s) 206
BtsCI GGATG 1 cut(s) 670
BtsI GCAGTG 2 cut(s) 189, 265
BtsIMutI CAGTG 2 cut(s) 189, 265
Cac8I GCNNGC 2 cut(s) 138, 591
CciI TCATGA 1 cut(s) 598
Cfr10I RCCGGY 2 cut(s) 322, 589
Cfr13I GGNCC 3 cut(s) 647, 685, 720
Cfr42I CCGCGG 1 cut(s) 336
ClaI ATCGAT 1 cut(s) 703
CspAI ACCGGT 1 cut(s) 322
CspCI CAANNNNNGTGG 2 cut(s) 67, 102
CviAII CATG 8 cut(s) 210, 454, 463, 499, 541, 599, 697, 712
DpnI GATC 6 cut(s) 43, 160, 578, 597, 606, 612
DpnII GATC 6 cut(s) 41, 158, 576, 595, 604, 610
EaeI YGGCCR 1 cut(s) 501
Eam1104I CTCTTC 3 cut(s) 461, 669, 747
EarI CTCTTC 3 cut(s) 461, 669, 747
Eco130I CCWWGG 1 cut(s) 534
Eco31I GGTCTC 2 cut(s) 113, 734
Eco47I GGWCC 2 cut(s) 647, 720
EcoRI GAATTC 1 cut(s) 634
EcoRII CCWGG 1 cut(s) 366
EcoT14I CCWWGG 1 cut(s) 534
EcoT22I ATGCAT 1 cut(s) 702
ErhI CCWWGG 1 cut(s) 534
FaeI CATG 8 cut(s) 213, 457, 466, 502, 544, 602, 700, 715
FaqI GGGAC 1 cut(s) 148
FatI CATG 8 cut(s) 209, 453, 462, 498, 540, 598, 696, 711
FauNDI CATATG 1 cut(s) 390
FbaI TGATCA 1 cut(s) 595
Fnu4HI GCNGC 5 cut(s) 333, 442, 504, 560, 587
FokI GGATG 1 cut(s) 677
Fsp4HI GCNGC 5 cut(s) 333, 442, 504, 560, 587
FspBI CTAG 1 cut(s) 201
GluI GCNGC 5 cut(s) 333, 442, 504, 560, 587
GsuI CTGGAG 1 cut(s) 273
HaeIII GGCC 3 cut(s) 503, 539, 686
HapII CCGG 4 cut(s) 166, 323, 567, 590
Hin1II CATG 8 cut(s) 213, 457, 466, 502, 544, 602, 700, 715
HpaII CCGG 4 cut(s) 166, 323, 567, 590
HphI GGTGA 3 cut(s) 311, 352, 508
Hpy166II GTNNAC 2 cut(s) 373, 448
Hpy188I TCNGA 2 cut(s) 496, 640
Hpy188III TCNNGA 5 cut(s) 39, 156, 308, 599, 608
Hpy8I GTNNAC 2 cut(s) 373, 448
HpyCH4III ACNGT 1 cut(s) 484
HpyCH4IV ACGT 3 cut(s) 205, 235, 450
HpyCH4V TGCA 7 cut(s) 182, 209, 270, 419, 544, 586, 700
HpyF10VI GCNNNNNNNGC 3 cut(s) 188, 416, 556
HpySE526I ACGT 3 cut(s) 205, 235, 450
Hsp92II CATG 8 cut(s) 213, 457, 466, 502, 544, 602, 700, 715
KroI GCCGGC 1 cut(s) 589
KroNI GCCGGC 1 cut(s) 591
Ksp22I TGATCA 1 cut(s) 595
KspI CCGCGG 1 cut(s) 336
Kzo9I GATC 6 cut(s) 41, 158, 576, 595, 604, 610
Lsp1109I GCAGC 3 cut(s) 453, 571, 598
LweI GCATC 3 cut(s) 238, 333, 709
MaeI CTAG 1 cut(s) 201
MaeII ACGT 3 cut(s) 205, 235, 450
MaeIII GTNAC 2 cut(s) 274, 484
MalI GATC 6 cut(s) 43, 160, 578, 597, 606, 612
MboI GATC 6 cut(s) 41, 158, 576, 595, 604, 610
MboII GAAGA 5 cut(s) 51, 478, 483, 686, 764
MflI RGATCY 1 cut(s) 41
MhlI GDGCHC 1 cut(s) 138
MluCI AATT 3 cut(s) 141, 634, 658
MmeI TCCRAC 3 cut(s) 34, 96, 717
MnlI CCTC 8 cut(s) 42, 73, 248, 251, 331, 462, 524, 748
Mph1103I ATGCAT 1 cut(s) 702
MroNI GCCGGC 1 cut(s) 589
MseI TTAA 1 cut(s) 624
MspA1I CMGCKG 1 cut(s) 335
MspI CCGG 4 cut(s) 166, 323, 567, 590
MspR9I CCNGG 2 cut(s) 166, 368
MvaI CCWGG 1 cut(s) 368
MvnI CGCG 1 cut(s) 335
MwoI GCNNNNNNNGC 3 cut(s) 188, 416, 556
NaeI GCCGGC 1 cut(s) 591
NciI CCSGG 1 cut(s) 166
NdeI CATATG 1 cut(s) 390
NdeII GATC 6 cut(s) 41, 158, 576, 595, 604, 610
NgoMIV GCCGGC 1 cut(s) 589
NlaIII CATG 8 cut(s) 213, 457, 466, 502, 544, 602, 700, 715
NlaIV GGNNCC 3 cut(s) 291, 648, 687
NmuCI GTSAC 2 cut(s) 274, 484
NsiI ATGCAT 1 cut(s) 702
PagI TCATGA 1 cut(s) 598
PdiI GCCGGC 1 cut(s) 591
PflMI CCANNNNNTGG 1 cut(s) 430
PinAI ACCGGT 1 cut(s) 322
PkrI GCNGC 5 cut(s) 334, 443, 505, 561, 588
PshAI GACNNNNGTC 1 cut(s) 645
PshBI ATTAAT 1 cut(s) 624
Psp6I CCWGG 1 cut(s) 366
PspGI CCWGG 1 cut(s) 366
PspN4I GGNNCC 3 cut(s) 291, 648, 687
PspPI GGNCC 3 cut(s) 647, 685, 720
PstI CTGCAG 1 cut(s) 421
PsuI RGATCY 1 cut(s) 41
SacII CCGCGG 1 cut(s) 336
SaqAI TTAA 1 cut(s) 624
SatI GCNGC 5 cut(s) 333, 442, 504, 560, 587
Sau3AI GATC 6 cut(s) 41, 158, 576, 595, 604, 610
Sau96I GGNCC 3 cut(s) 647, 685, 720
ScrFI CCNGG 2 cut(s) 166, 368
SduI GDGCHC 1 cut(s) 138
SfaNI GCATC 3 cut(s) 238, 333, 709
SfcI CTRYAG 2 cut(s) 417, 480
Sfr303I CCGCGG 1 cut(s) 336
SgrBI CCGCGG 1 cut(s) 336
SinI GGWCC 2 cut(s) 647, 720
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
Sse9I AATT 3 cut(s) 141, 634, 658
SsiI CCGC 5 cut(s) 297, 333, 335, 504, 519
SspMI CTAG 1 cut(s) 201
StyD4I CCNGG 2 cut(s) 164, 366
StyI CCWWGG 1 cut(s) 534
TaaI ACNGT 1 cut(s) 484
TaiI ACGT 3 cut(s) 208, 238, 453
TaqI TCGA 6 cut(s) 38, 155, 307, 348, 672, 703
TasI AATT 3 cut(s) 141, 634, 658
TauI GCSGC 2 cut(s) 335, 506
Tru1I TTAA 1 cut(s) 624
Tru9I TTAA 1 cut(s) 624
TscAI CASTG 2 cut(s) 189, 272
TseFI GTSAC 2 cut(s) 274, 484
TseI GCWGC 3 cut(s) 441, 559, 586
Tsp45I GTSAC 2 cut(s) 274, 484
TspDTI ATGAA 6 cut(s) 329, 333, 479, 647, 681, 685
TspGWI ACGGA 1 cut(s) 121
TspRI CASTG 2 cut(s) 189, 272
Van91I CCANNNNNTGG 1 cut(s) 430
VpaK11BI GGWCC 2 cut(s) 647, 720
VspI ATTAAT 1 cut(s) 624
XapI RAATTY 2 cut(s) 634, 658
XspI CTAG 1 cut(s) 201
Zsp2I ATGCAT 1 cut(s) 702
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.