MD16G1031400.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0003619
Physical Location & Seq
Reverse (-)
2229450 .. 2230019
570 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1031400.v1.1.491

Sequence Viewer

Length: 570 bp
ATGGGAAACTACATCTCATGCACTTTGGCCACGCCGATGATCAAGAGCTCCAAGTCAGCGAGGGTGATCTTCCCCAACGGCGACGTCCGGCAGTTCCGGGAGACGACCAAGGCGGCGGAGCTCATGCTGGAGTGTCCCAACACCTTCTTGGCCAACTCGACTTCTCTCCACATTGGCAGGAGATTCTCCGCCCTCAGCGCCGACGAGGAGTTGGAGTTTGGGAACGCATACATCATGTTTCCAATGAGGAGGCTCAGCTCCATAGTCACGGCGGCCGACATGGCCGTGTTCTTCATGGCGGCTAACTCCGCTGCCAAGCGTATATCCGGCTGGAAGGTTAGGATATTGCCGGAGAGTGGTTGCGGAGGAGGAGAAGTGGCGGCGGTGGAGAGTGGGAAAGAAAATCATGGTGATAAAGATCATGAAGGTGATCATCAAGGTCCGTTGCGGCTGAGCTTGGACGGAGTTGAGGGGTTGCAAGTGGCAGAGTTTCGGTACAGATTAGCTGCGTCTAGGTCAAGGAAGCCGGTGTTGGAAACCATCAAAGAAGAGCCGATTGGTTCTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

190

Amino Acids

20.65

Weight (kDa)

7.75

Isoelectric Point (pI)

43.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 183 1.1e-36 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 87
AcoI YGGCCR 4 cut(s) 27, 150, 273, 282
AcyI GRCGYC 1 cut(s) 84
AfaI GTAC 1 cut(s) 497
AfiI CCNNNNNNNGG 1 cut(s) 356
AjuI GAANNNNNNNTTGG 3 cut(s) 515, 540, 547
AluBI AGCT 5 cut(s) 48, 121, 258, 456, 506
AluI AGCT 5 cut(s) 48, 121, 258, 456, 506
Alw21I GWGCWC 2 cut(s) 50, 123
Alw26I GTCTC 1 cut(s) 95
AoxI GGCC 4 cut(s) 27, 150, 273, 282
ApeKI GCWGC 2 cut(s) 311, 506
AspLEI GCGC 1 cut(s) 200
AspS9I GGNCC 1 cut(s) 440
AsuC2I CCSGG 1 cut(s) 98
AsuHPI GGTGA 3 cut(s) 76, 422, 440
AvaII GGWCC 1 cut(s) 440
BalI TGGCCA 2 cut(s) 29, 152
BanII GRGCYC 2 cut(s) 50, 123
Bbv12I GWGCWC 2 cut(s) 50, 123
BbvCI CCTCAGC 1 cut(s) 194
BbvI GCAGC 2 cut(s) 298, 493
BccI CCATC 1 cut(s) 548
BceAI ACGGC 3 cut(s) 94, 269, 285
BclI TGATCA 2 cut(s) 39, 430
BcnI CCSGG 1 cut(s) 98
BcoDI GTCTC 1 cut(s) 95
BfaI CTAG 2 cut(s) 513, 564
BfoI RGCGCY 1 cut(s) 201
BglI GCCNNNNNGGC 1 cut(s) 281
BisI GCNGC 7 cut(s) 114, 273, 300, 312, 381, 449, 507
BlpI GCTNAGC 2 cut(s) 254, 452
BlsI GCNGC 7 cut(s) 115, 274, 301, 313, 382, 450, 508
Bme1390I CCNGG 1 cut(s) 98
Bme18I GGWCC 1 cut(s) 440
BmgT120I GGNCC 1 cut(s) 440
BmrFI CCNGG 1 cut(s) 98
BpmI CTGGAG 1 cut(s) 149
Bpu10I CCTNAGC 1 cut(s) 194
Bpu1102I GCTNAGC 2 cut(s) 254, 452
BpuMI CCSGG 1 cut(s) 98
BsaBI GATNNNNATC 1 cut(s) 417
BsaHI GRCGYC 1 cut(s) 84
BsaJI CCNNGG 1 cut(s) 108
BsaXI ACNNNNNCTCC 4 cut(s) 200, 230, 360, 390
Bsc4I CCNNNNNNNGG 1 cut(s) 356
Bse118I RCCGGY 1 cut(s) 526
Bse8I GATNNNNATC 1 cut(s) 417
BseDI CCNNGG 1 cut(s) 108
BseJI GATNNNNATC 1 cut(s) 417
BseLI CCNNNNNNNGG 1 cut(s) 356
BseMII CTCAG 3 cut(s) 208, 268, 443
BseRI GAGGAG 4 cut(s) 221, 262, 381, 384
BseX3I CGGCCG 1 cut(s) 273
BseXI GCAGC 2 cut(s) 298, 493
Bsh1285I CGRYCG 1 cut(s) 276
BshFI GGCC 4 cut(s) 29, 152, 275, 284
BsiEI CGRYCG 1 cut(s) 276
BsiHKAI GWGCWC 2 cut(s) 50, 123
BsiSI CCGG 5 cut(s) 88, 97, 327, 350, 527
BslFI GGGAC 1 cut(s) 120
BslI CCNNNNNNNGG 1 cut(s) 356
BsmAI GTCTC 1 cut(s) 95
BsmBI CGTCTC 1 cut(s) 95
BsmFI GGGAC 1 cut(s) 120
BsnI GGCC 4 cut(s) 29, 152, 275, 284
Bsp1286I GDGCHC 2 cut(s) 50, 123
Bsp143I GATC 4 cut(s) 39, 66, 418, 430
Bsp1720I GCTNAGC 2 cut(s) 254, 452
BspANI GGCC 4 cut(s) 29, 152, 275, 284
BspCNI CTCAG 3 cut(s) 207, 267, 444
BspHI TCATGA 1 cut(s) 421
BspQI GCTCTTC 1 cut(s) 543
BsrFI RCCGGY 1 cut(s) 526
BssAI RCCGGY 1 cut(s) 526
BssECI CCNNGG 1 cut(s) 108
BssMI GATC 4 cut(s) 39, 66, 418, 430
BssNI GRCGYC 1 cut(s) 84
BssT1I CCWWGG 1 cut(s) 108
Bst6I CTCTTC 1 cut(s) 543
BstACI GRCGYC 1 cut(s) 84
BstDEI CTNAG 3 cut(s) 194, 254, 452
BstH2I RGCGCY 1 cut(s) 201
BstHHI GCGC 1 cut(s) 200
BstKTI GATC 4 cut(s) 42, 69, 421, 433
BstMAI GTCTC 1 cut(s) 95
BstMBI GATC 4 cut(s) 39, 66, 418, 430
BstMCI CGRYCG 1 cut(s) 276
BstMWI GCNNNNNNNGC 3 cut(s) 197, 281, 308
BstSCI CCNGG 1 cut(s) 96
BstV1I GCAGC 2 cut(s) 298, 493
BstZI CGGCCG 1 cut(s) 273
BsuRI GGCC 4 cut(s) 29, 152, 275, 284
CciI TCATGA 1 cut(s) 421
CfoI GCGC 1 cut(s) 200
Cfr10I RCCGGY 1 cut(s) 526
Cfr13I GGNCC 1 cut(s) 440
CseI GACGC 1 cut(s) 498
Csp6I GTAC 1 cut(s) 496
CviAII CATG 7 cut(s) 18, 124, 235, 280, 295, 407, 422
CviQI GTAC 1 cut(s) 496
DdeI CTNAG 3 cut(s) 194, 254, 452
DpnI GATC 4 cut(s) 41, 68, 420, 432
DpnII GATC 4 cut(s) 39, 66, 418, 430
EaeI YGGCCR 4 cut(s) 27, 150, 273, 282
EagI CGGCCG 1 cut(s) 273
Eam1104I CTCTTC 1 cut(s) 543
EarI CTCTTC 1 cut(s) 543
EciI GGCGGA 2 cut(s) 131, 178
Ecl136II GAGCTC 2 cut(s) 48, 121
EclXI CGGCCG 1 cut(s) 273
Eco130I CCWWGG 1 cut(s) 108
Eco24I GRGCYC 2 cut(s) 50, 123
Eco47I GGWCC 1 cut(s) 440
Eco52I CGGCCG 1 cut(s) 273
Eco53kI GAGCTC 2 cut(s) 48, 121
EcoICRI GAGCTC 2 cut(s) 48, 121
EcoT14I CCWWGG 1 cut(s) 108
EcoT38I GRGCYC 2 cut(s) 50, 123
ErhI CCWWGG 1 cut(s) 108
Esp3I CGTCTC 1 cut(s) 95
FaeI CATG 7 cut(s) 21, 127, 238, 283, 298, 410, 425
FaqI GGGAC 1 cut(s) 120
FatI CATG 7 cut(s) 17, 123, 234, 279, 294, 406, 421
FbaI TGATCA 2 cut(s) 39, 430
Fnu4HI GCNGC 7 cut(s) 114, 273, 300, 312, 381, 449, 507
FriOI GRGCYC 2 cut(s) 50, 123
Fsp4HI GCNGC 7 cut(s) 114, 273, 300, 312, 381, 449, 507
FspBI CTAG 2 cut(s) 513, 564
GlaI GCGC 1 cut(s) 199
GluI GCNGC 7 cut(s) 114, 273, 300, 312, 381, 449, 507
GsuI CTGGAG 1 cut(s) 149
HaeII RGCGCY 1 cut(s) 201
HaeIII GGCC 4 cut(s) 29, 152, 275, 284
HapII CCGG 5 cut(s) 88, 97, 327, 350, 527
HgaI GACGC 1 cut(s) 498
HhaI GCGC 1 cut(s) 200
Hin1I GRCGYC 1 cut(s) 84
Hin1II CATG 7 cut(s) 21, 127, 238, 283, 298, 410, 425
Hin6I GCGC 1 cut(s) 198
HinP1I GCGC 1 cut(s) 198
HinfI GANTC 1 cut(s) 183
HpaII CCGG 5 cut(s) 88, 97, 327, 350, 527
HphI GGTGA 3 cut(s) 76, 422, 440
Hpy188III TCNNGA 3 cut(s) 43, 422, 564
Hpy99I CGWCG 2 cut(s) 86, 206
HpyAV CCTTC 3 cut(s) 154, 328, 419
HpyCH4IV ACGT 1 cut(s) 84
HpyCH4V TGCA 2 cut(s) 21, 478
HpyF10VI GCNNNNNNNGC 3 cut(s) 197, 281, 308
HpyF3I CTNAG 3 cut(s) 194, 254, 452
HpySE526I ACGT 1 cut(s) 84
Hsp92I GRCGYC 1 cut(s) 84
Hsp92II CATG 7 cut(s) 21, 127, 238, 283, 298, 410, 425
HspAI GCGC 1 cut(s) 198
Ksp22I TGATCA 2 cut(s) 39, 430
Kzo9I GATC 4 cut(s) 39, 66, 418, 430
LguI GCTCTTC 1 cut(s) 543
LmnI GCTCC 3 cut(s) 53, 118, 263
LpnPI CCDG 8 cut(s) 101, 110, 113, 163, 316, 340, 363, 540
Lsp1109I GCAGC 2 cut(s) 298, 493
MaeI CTAG 2 cut(s) 513, 564
MaeII ACGT 1 cut(s) 84
MaeIII GTNAC 1 cut(s) 265
MalI GATC 4 cut(s) 41, 68, 420, 432
MboI GATC 4 cut(s) 39, 66, 418, 430
MboII GAAGA 3 cut(s) 61, 283, 560
MhlI GDGCHC 2 cut(s) 50, 123
MlsI TGGCCA 2 cut(s) 29, 152
MluNI TGGCCA 2 cut(s) 29, 152
MmeI TCCRAC 2 cut(s) 192, 513
MnlI CCTC 8 cut(s) 54, 199, 203, 240, 243, 359, 362, 463
Mox20I TGGCCA 2 cut(s) 29, 152
MscI TGGCCA 2 cut(s) 29, 152
MslI CAYNNNNRTG 3 cut(s) 35, 284, 426
Msp20I TGGCCA 2 cut(s) 29, 152
MspA1I CMGCKG 1 cut(s) 311
MspI CCGG 5 cut(s) 88, 97, 327, 350, 527
MspR9I CCNGG 1 cut(s) 98
MwoI GCNNNNNNNGC 3 cut(s) 197, 281, 308
NciI CCSGG 1 cut(s) 98
NdeII GATC 4 cut(s) 39, 66, 418, 430
NlaIII CATG 7 cut(s) 21, 127, 238, 283, 298, 410, 425
NmuCI GTSAC 1 cut(s) 265
PagI TCATGA 1 cut(s) 421
PciSI GCTCTTC 1 cut(s) 543
PfeI GAWTC 1 cut(s) 183
PfoI TCCNGGA 1 cut(s) 96
PkrI GCNGC 7 cut(s) 115, 274, 301, 313, 382, 450, 508
Psp124BI GAGCTC 2 cut(s) 50, 123
PspPI GGNCC 1 cut(s) 440
RsaI GTAC 1 cut(s) 497
RsaNI GTAC 1 cut(s) 496
RseI CAYNNNNRTG 3 cut(s) 35, 284, 426
SacI GAGCTC 2 cut(s) 50, 123
SapI GCTCTTC 1 cut(s) 543
SatI GCNGC 7 cut(s) 114, 273, 300, 312, 381, 449, 507
Sau3AI GATC 4 cut(s) 39, 66, 418, 430
Sau96I GGNCC 1 cut(s) 440
ScrFI CCNGG 1 cut(s) 98
SduI GDGCHC 2 cut(s) 50, 123
SfiI GGCCNNNNNGGCC 1 cut(s) 281
SinI GGWCC 1 cut(s) 440
SmiMI CAYNNNNRTG 3 cut(s) 35, 284, 426
SspMI CTAG 2 cut(s) 513, 564
SstI GAGCTC 2 cut(s) 50, 123
StyD4I CCNGG 1 cut(s) 96
StyI CCWWGG 1 cut(s) 108
TaiI ACGT 1 cut(s) 87
TaqI TCGA 1 cut(s) 158
TauI GCSGC 5 cut(s) 116, 275, 302, 383, 451
TfiI GAWTC 1 cut(s) 183
TseFI GTSAC 1 cut(s) 265
TseI GCWGC 2 cut(s) 311, 506
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 2 cut(s) 283, 438
TspGWI ACGGA 2 cut(s) 432, 477
VpaK11BI GGWCC 1 cut(s) 440
XbaI TCTAGA 1 cut(s) 563
XcmI CCANNNNNNNNNTGG 1 cut(s) 145
XspI CTAG 2 cut(s) 513, 564
ZraI GACGTC 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.