Rorug04G0271300

Repressor of RNA polymerase III transcription

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000924
Physical Location & Seq
Reverse (-)
44361810 .. 44364981
3172 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0271300.1

Sequence Viewer

Length: 1134 bp
ATGGTGCTGTTTCCATCTTTCCTAGATGGGTTGGGAAGAACCGTCTCGATCAAGAAGACCAAGAATTGCCATGGTGATGTGGCAAAGGAGACTGCAGAAGCATTGGCGAAGGAAGCAAAAAAGAATGAGCTGGTTTTGAGTTCTTCTGGGATTGTTGACTCTGACAAGTCTAACAATTTTTCTTCTGTTTGCTCCAAGAGAGGCCAAAAGGGTATCAATCAGGATTGCTTAGTCATTTGGGAGGAATTTGGGTGCCAAGAAGACATGATCTTTTGTGGAGTGTTTGATGGGCATGGACAGTGGGGACATCTTGTTAGCAAAAGGGTCAGGCAATCTATGCCTTCTTCTTTGTTGTGCAATTGGCAAGAAACTCTCAGTTTAACATCACTTGACCTGAATTTTAAAATGGAGATGGATCGAAATCTCCATCGATATGATACATGGAAGCAGTCCTTTCTTAAAACATACGCTGCCATTGACCAGGAGCTTAAGCATTCTGCTAAAATCGATTCCTATCGAAGTGGTACTACAGCGTTGACAATTATTAAGCAGGGTGAACTTCTCATCATAGCACATGTTGGCGATTCTCGGGCTGTTTTGGCCTCCACTTCAGAAGATGGGAGCTTGGTACCTATCCAGCTTACAGTCGACTTCAAGCCTAACTTACCTCAGGAGGCTGAGAGAATCAGAAGGTCCAATGGACGAGTGTTTTGTCTACATGATGAACCGGGGACTTATAGGGTGTGGATGCCAAATGGCAAGACACCAGGACTGGCAGTATCAAGAGCTTTTGGTGACTATTGTGTTAAGGACTTTGGGCTTATTTCTGTGCCTGATGTGAGCCAAAGATACATAACCAGCAAAGACCAGTTTGTCATTCTAGCAACAGATGGGGTATGGGATGTTATCTCCAACAAAGAAGCTATACAAATAGTTTCTTCGGCACCAGATAGGAACAAAGCAGCAAAAAGACTGGTGGCATGTGCTGCTCTTGCATGGAAGTCTAAGAGAAGAGGCATTGCAATGGATGACATCTCTGCTATTTGCCTGTTCTTTCACCCTAACTCATCATCGCAACAAACCAACCCTTTGGTCTCTGAATATACTGGTGTGAAGACATCTGGGTGTATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Maf1

Protein Analysis

377

Amino Acids

41.76

Weight (kDa)

8.4

Isoelectric Point (pI)

38.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 73 - 328 1.2e-41 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 872
Acc65I GGTACC 1 cut(s) 628
AccB1I GGYRCC 3 cut(s) 252, 628, 943
AccI GTMKAC 2 cut(s) 648, 715
AclWI GGATC 1 cut(s) 423
AcsI RAATTY 2 cut(s) 245, 397
AcuI CTGAAG 1 cut(s) 594
AfaI GTAC 2 cut(s) 526, 630
AflII CTTAAG 1 cut(s) 488
AflIII ACRYGT 1 cut(s) 574
AgsI TTSAA 1 cut(s) 655
AjnI CCWGG 2 cut(s) 480, 766
AluBI AGCT 6 cut(s) 130, 487, 624, 640, 788, 923
AluI AGCT 6 cut(s) 130, 487, 624, 640, 788, 923
Alw26I GTCTC 3 cut(s) 49, 83, 1099
AlwI GGATC 1 cut(s) 423
Ama87I CYCGRG 1 cut(s) 588
AoxI GGCC 2 cut(s) 202, 600
ApeKI GCWGC 3 cut(s) 470, 962, 986
ApoI RAATTY 2 cut(s) 245, 397
ArsI GACNNNNNNTTYG 2 cut(s) 693, 725
Asp718I GGTACC 1 cut(s) 628
AspS9I GGNCC 1 cut(s) 693
AsuC2I CCSGG 1 cut(s) 729
AsuHPI GGTGA 4 cut(s) 86, 566, 806, 1049
AvaI CYCGRG 1 cut(s) 588
AvaII GGWCC 1 cut(s) 693
AxyI CCTNAGG 1 cut(s) 669
BanI GGYRCC 3 cut(s) 252, 628, 943
BarI GAAGNNNNNNTAC 2 cut(s) 511, 543
BbsI GAAGAC 3 cut(s) 62, 267, 1121
BbvI GCAGC 3 cut(s) 457, 973, 974
BccI CCATC 7 cut(s) 20, 22, 281, 406, 435, 611, 884
BciT130I CCWGG 2 cut(s) 482, 768
BcnI CCSGG 1 cut(s) 729
BcoDI GTCTC 3 cut(s) 49, 83, 1099
BfaI CTAG 2 cut(s) 23, 881
BfmI CTRYAG 2 cut(s) 93, 528
BfrI CTTAAG 1 cut(s) 488
BisI GCNGC 3 cut(s) 471, 963, 987
BlsI GCNGC 3 cut(s) 472, 964, 988
Bme1390I CCNGG 3 cut(s) 482, 729, 768
Bme18I GGWCC 1 cut(s) 693
BmeT110I CYCGRG 1 cut(s) 588
BmgT120I GGNCC 1 cut(s) 693
BmiI GGNNCC 3 cut(s) 254, 630, 945
BmrFI CCNGG 3 cut(s) 482, 729, 768
BmsI GCATC 1 cut(s) 738
BpiI GAAGAC 3 cut(s) 62, 267, 1121
BpuMI CCSGG 1 cut(s) 729
Bsa29I ATCGAT 2 cut(s) 430, 507
BsaBI GATNNNNATC 2 cut(s) 420, 513
BsaI GGTCTC 1 cut(s) 1099
BsaJI CCNNGG 2 cut(s) 70, 728
Bse1I ACTGG 4 cut(s) 777, 868, 978, 1111
Bse21I CCTNAGG 1 cut(s) 669
Bse3DI GCAATG 2 cut(s) 1017, 1029
Bse8I GATNNNNATC 2 cut(s) 420, 513
BseBI CCWGG 2 cut(s) 482, 768
BseCI ATCGAT 2 cut(s) 430, 507
BseDI CCNNGG 2 cut(s) 70, 728
BseGI GGATG 3 cut(s) 753, 907, 1033
BseJI GATNNNNATC 2 cut(s) 420, 513
BseMI GCAATG 2 cut(s) 1017, 1029
BseMII CTCAG 3 cut(s) 388, 669, 683
BseNI ACTGG 4 cut(s) 777, 868, 978, 1111
BseXI GCAGC 3 cut(s) 457, 973, 974
BshFI GGCC 2 cut(s) 204, 602
BshNI GGYRCC 3 cut(s) 252, 628, 943
BshVI ATCGAT 2 cut(s) 430, 507
BsiHKCI CYCGRG 1 cut(s) 588
BsiSI CCGG 1 cut(s) 728
BslFI GGGAC 2 cut(s) 318, 745
BsmAI GTCTC 3 cut(s) 49, 83, 1099
BsmBI CGTCTC 1 cut(s) 49
BsmFI GGGAC 2 cut(s) 318, 745
BsmI GAATGC 1 cut(s) 493
BsnI GGCC 2 cut(s) 204, 602
Bso31I GGTCTC 1 cut(s) 1099
BsoBI CYCGRG 1 cut(s) 588
Bsp143I GATC 3 cut(s) 48, 267, 415
Bsp19I CCATGG 1 cut(s) 70
BspANI GGCC 2 cut(s) 204, 602
BspCNI CTCAG 3 cut(s) 387, 670, 682
BspDI ATCGAT 2 cut(s) 430, 507
BspLI GGNNCC 3 cut(s) 254, 630, 945
BspMAI CTGCAG 1 cut(s) 97
BspPI GGATC 1 cut(s) 423
BspT107I GGYRCC 3 cut(s) 252, 628, 943
BspTI CTTAAG 1 cut(s) 488
BspTNI GGTCTC 1 cut(s) 1099
BsrDI GCAATG 2 cut(s) 1017, 1029
BsrI ACTGG 4 cut(s) 777, 868, 978, 1111
BssECI CCNNGG 2 cut(s) 70, 728
BssMI GATC 3 cut(s) 48, 267, 415
BssT1I CCWWGG 1 cut(s) 70
Bst2UI CCWGG 2 cut(s) 482, 768
Bst4CI ACNGT 3 cut(s) 43, 300, 646
Bst6I CTCTTC 1 cut(s) 1006
BstAFI CTTAAG 1 cut(s) 488
BstAPI GCANNNNNTGC 2 cut(s) 337, 986
BstDEI CTNAG 5 cut(s) 229, 374, 669, 678, 1005
BstDSI CCRYGG 1 cut(s) 70
BstF5I GGATG 3 cut(s) 753, 907, 1033
BstKTI GATC 3 cut(s) 51, 270, 418
BstMAI GTCTC 3 cut(s) 49, 83, 1099
BstMBI GATC 3 cut(s) 48, 267, 415
BstMWI GCNNNNNNNGC 5 cut(s) 113, 337, 599, 986, 992
BstNI CCWGG 2 cut(s) 482, 768
BstNSI RCATGY 2 cut(s) 578, 984
BstSCI CCNGG 3 cut(s) 480, 727, 766
BstSFI CTRYAG 2 cut(s) 93, 528
BstV1I GCAGC 3 cut(s) 457, 973, 974
BstV2I GAAGAC 3 cut(s) 62, 267, 1121
BstXI CCANNNNNNTGG 1 cut(s) 1090
Bsu15I ATCGAT 2 cut(s) 430, 507
Bsu36I CCTNAGG 1 cut(s) 669
BsuRI GGCC 2 cut(s) 204, 602
BsuTUI ATCGAT 2 cut(s) 430, 507
BtgI CCRYGG 1 cut(s) 70
BtgZI GCGATG 1 cut(s) 1056
BtsCI GGATG 3 cut(s) 753, 907, 1033
BtsIMutI CAGTG 1 cut(s) 305
Cfr13I GGNCC 1 cut(s) 693
ClaI ATCGAT 2 cut(s) 430, 507
Csp6I GTAC 2 cut(s) 525, 629
CviAII CATG 8 cut(s) 71, 265, 293, 441, 575, 719, 981, 996
CviQI GTAC 2 cut(s) 525, 629
DdeI CTNAG 5 cut(s) 229, 374, 669, 678, 1005
DpnI GATC 3 cut(s) 50, 269, 417
DpnII GATC 3 cut(s) 48, 267, 415
DraI TTTAAA 1 cut(s) 403
DrdI GACNNNNNNGTC 1 cut(s) 872
DseDI GACNNNNNNGTC 1 cut(s) 872
Eam1104I CTCTTC 1 cut(s) 1006
EarI CTCTTC 1 cut(s) 1006
Eco130I CCWWGG 1 cut(s) 70
Eco31I GGTCTC 1 cut(s) 1099
Eco47I GGWCC 1 cut(s) 693
Eco57I CTGAAG 1 cut(s) 594
Eco81I CCTNAGG 1 cut(s) 669
Eco88I CYCGRG 1 cut(s) 588
EcoRII CCWGG 2 cut(s) 480, 766
EcoT14I CCWWGG 1 cut(s) 70
ErhI CCWWGG 1 cut(s) 70
Esp3I CGTCTC 1 cut(s) 49
FaeI CATG 8 cut(s) 74, 268, 296, 444, 578, 722, 984, 999
FalI AAGNNNNNCTT 4 cut(s) 437, 469, 647, 679
FaqI GGGAC 2 cut(s) 318, 745
FatI CATG 8 cut(s) 70, 264, 292, 440, 574, 718, 980, 995
FblI GTMKAC 2 cut(s) 648, 715
Fnu4HI GCNGC 3 cut(s) 471, 963, 987
FokI GGATG 3 cut(s) 760, 914, 1040
Fsp4HI GCNGC 3 cut(s) 471, 963, 987
FspBI CTAG 2 cut(s) 23, 881
GluI GCNGC 3 cut(s) 471, 963, 987
HaeIII GGCC 2 cut(s) 204, 602
HapII CCGG 1 cut(s) 728
Hin1II CATG 8 cut(s) 74, 268, 296, 444, 578, 722, 984, 999
HincII GTYRAC 3 cut(s) 157, 537, 649
HindII GTYRAC 3 cut(s) 157, 537, 649
HinfI GANTC 4 cut(s) 158, 509, 584, 684
HpaII CCGG 1 cut(s) 728
HphI GGTGA 4 cut(s) 86, 566, 806, 1049
Hpy166II GTNNAC 5 cut(s) 157, 537, 557, 649, 716
Hpy188I TCNGA 4 cut(s) 163, 613, 689, 1099
Hpy188III TCNNGA 5 cut(s) 46, 52, 221, 671, 783
Hpy8I GTNNAC 5 cut(s) 157, 537, 557, 649, 716
HpyAV CCTTC 3 cut(s) 103, 351, 684
HpyCH4III ACNGT 3 cut(s) 43, 300, 646
HpyCH4V TGCA 4 cut(s) 95, 357, 995, 1022
HpyF10VI GCNNNNNNNGC 5 cut(s) 113, 337, 599, 986, 992
HpyF3I CTNAG 5 cut(s) 229, 374, 669, 678, 1005
Hsp92II CATG 8 cut(s) 74, 268, 296, 444, 578, 722, 984, 999
KpnI GGTACC 1 cut(s) 632
Kzo9I GATC 3 cut(s) 48, 267, 415
LmnI GCTCC 3 cut(s) 197, 484, 621
Lsp1109I GCAGC 3 cut(s) 457, 973, 974
LweI GCATC 1 cut(s) 738
MaeI CTAG 2 cut(s) 23, 881
MaeIII GTNAC 1 cut(s) 794
MalI GATC 3 cut(s) 50, 269, 417
MboI GATC 3 cut(s) 48, 267, 415
MfeI CAATTG 1 cut(s) 358
MluCI AATT 6 cut(s) 64, 175, 245, 358, 397, 540
MlyI GAGTC 1 cut(s) 152
MmeI TCCRAC 1 cut(s) 936
MnlI CCTC 6 cut(s) 194, 235, 613, 667, 678, 1007
MseI TTAA 6 cut(s) 380, 402, 459, 489, 546, 807
MslI CAYNNNNRTG 4 cut(s) 75, 432, 1022, 1123
MspCI CTTAAG 1 cut(s) 488
MspI CCGG 1 cut(s) 728
MspR9I CCNGG 3 cut(s) 482, 729, 768
MunI CAATTG 1 cut(s) 358
Mva1269I GAATGC 1 cut(s) 493
MvaI CCWGG 2 cut(s) 482, 768
MwoI GCNNNNNNNGC 5 cut(s) 113, 337, 599, 986, 992
NciI CCSGG 1 cut(s) 729
NcoI CCATGG 1 cut(s) 70
NdeII GATC 3 cut(s) 48, 267, 415
NlaIII CATG 8 cut(s) 74, 268, 296, 444, 578, 722, 984, 999
NlaIV GGNNCC 3 cut(s) 254, 630, 945
NmuCI GTSAC 1 cut(s) 794
NspI RCATGY 2 cut(s) 578, 984
PciI ACATGT 1 cut(s) 574
PctI GAATGC 1 cut(s) 493
PfeI GAWTC 3 cut(s) 509, 584, 684
PkrI GCNGC 3 cut(s) 472, 964, 988
PleI GAGTC 1 cut(s) 152
PpsI GAGTC 1 cut(s) 152
PscI ACATGT 1 cut(s) 574
Psp6I CCWGG 2 cut(s) 480, 766
PspGI CCWGG 2 cut(s) 480, 766
PspN4I GGNNCC 3 cut(s) 254, 630, 945
PspPI GGNCC 1 cut(s) 693
PstI CTGCAG 1 cut(s) 97
RsaI GTAC 2 cut(s) 526, 630
RsaNI GTAC 2 cut(s) 525, 629
RseI CAYNNNNRTG 4 cut(s) 75, 432, 1022, 1123
SalI GTCGAC 1 cut(s) 647
SaqAI TTAA 6 cut(s) 380, 402, 459, 489, 546, 807
SatI GCNGC 3 cut(s) 471, 963, 987
Sau3AI GATC 3 cut(s) 48, 267, 415
Sau96I GGNCC 1 cut(s) 693
SchI GAGTC 1 cut(s) 152
ScrFI CCNGG 3 cut(s) 482, 729, 768
SfaNI GCATC 1 cut(s) 738
SfcI CTRYAG 2 cut(s) 93, 528
SinI GGWCC 1 cut(s) 693
SmiMI CAYNNNNRTG 4 cut(s) 75, 432, 1022, 1123
SmlI CTYRAG 1 cut(s) 488
SmoI CTYRAG 1 cut(s) 488
Sse9I AATT 6 cut(s) 64, 175, 245, 358, 397, 540
SspMI CTAG 2 cut(s) 23, 881
StyD4I CCNGG 3 cut(s) 480, 727, 766
StyI CCWWGG 1 cut(s) 70
TaaI ACNGT 3 cut(s) 43, 300, 646
TaqI TCGA 6 cut(s) 47, 418, 430, 507, 517, 648
TasI AATT 6 cut(s) 64, 175, 245, 358, 397, 540
TfiI GAWTC 3 cut(s) 509, 584, 684
Tru1I TTAA 6 cut(s) 380, 402, 459, 489, 546, 807
Tru9I TTAA 6 cut(s) 380, 402, 459, 489, 546, 807
TscAI CASTG 1 cut(s) 305
TseFI GTSAC 1 cut(s) 794
TseI GCWGC 3 cut(s) 470, 962, 986
Tsp45I GTSAC 1 cut(s) 794
TspDTI ATGAA 1 cut(s) 738
TspRI CASTG 1 cut(s) 305
Vha464I CTTAAG 1 cut(s) 488
VpaK11BI GGWCC 1 cut(s) 693
XapI RAATTY 2 cut(s) 245, 397
XceI RCATGY 2 cut(s) 578, 984
XmiI GTMKAC 2 cut(s) 648, 715
XspI CTAG 2 cut(s) 23, 881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.