Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0000924

Physical Location & Seq

Genomic Coordinates Reverse (-)

63754078 .. 63756900

Gene Structure 2823 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG326000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1647 bp

ATGGCTAGAATTCTCATCTTCTTCATGGAGCTTGTTCTATTATGCTTATGCATAGCAACAATGACGACGAAGGCAGAAGCAGAATGCATGATATATAAGGAGCGCAACCAACCCTTAAATACTAGAATCAATGACCTACTGAGCCGGATGACCTTGGAGGAAAAGATTGGCCAAATGACGCAGATAGACCGCAGTATTGCTTCACCTGAGGTGATGTACAAGTACAAGATTGGGAGTGTTTTGAGTGGGAGTGGGAGTGTGCCAGCAAAAAAGGCTTCTGCAGAAACTTGGATTAGAATGGTGAATGATTTTCAAAACGGATGTTTATCGACTCGGCTTGCTATTCCACTGATTTATGGTATTGATGCTATTCATGGCCACAACAATGTCTACAAGGCGACAATCTTTCCTCACAATATTGGACTGGGAGCTACCAGGGACCCTGAACTTGTTAAGAGGATTGGAGCTGCAACCGCGCTTGAAGTTAGAGCTACAGGAATCCATTATACCTTTGCACCTTGTGTAGCGGTTGGTAGAGATCCAAGATGGGGTCGTTGTTACGAAAGCTACAGTGAAGATCCTAAGCTTGTTCAAGCAATGACTGAGATCATACCTGGATTACAAGGAGAGATATCAGCTAACTCTAGAAAGGGTGCTCCCTATGTAGCAGGAAACGATAAGGTTGCAGCTTGTGCAAAGCATTATGTGGGAGATGGTGGAACAATCATGGGCCTCAATGAGAATAACACTGTGATAGATAGACATGGTTTACTGAGCATTCACATGCCACCCTGCTCCAGTGCAATTATCAAGGCTGTTGCAACCATTATGGGAATCAGTGCTGGCATTGATATGGTTATCATGGTTCCATACAACCATACAGAATTCATCAAGGATCTAACTTTCCATGTGAAAAATAAAATCATCCCCATGAGCAGAATTGACGATGCTGTGAAGAGAATTTTGCGGGTTAAGTTTACAATGGGGCTATTTGAGAAACCGTTGGCTGATCTCAGATTGGTCAACCAGCTGCTTGGCAGTCAGACATCAAAAATTCTTGTAGCTGGAAGTCATGCAGATAATCTTGGTTATCAGTGTGGTGGTTGGACCATTGAGTGGCAGGGACTAAGTGGCAACAACCTCACACGAGGTATCACAATTCTAACTGCCATAAGGAACACAGTTGCACCTGAAACCAAAGTAGTGTACAAGGAGAATCCTGATGCCAAGTTTGTGAAGTCAAATAACTTCTCCCATGCCATCGTTATGGTAGGAGAGCCACCCTATGCCGAGGGAGCTGGTGACAACCTAAACTTAACAATCCCTAAATCTGGCCAAAGAGTCATCAGAAATGTTTGCGGTGCAGCAAAATGTGTTGTTTGCCTCATCACCGGCCGTCCTGTAGTGATCAAACCATATCTTTCCATCATAGATACTCTTGTTGCAGCTTGGCTCCCAGGGACTGAAGGCCAAGGAGTTGCTGATGTTTTGTTTGGAGACTATGGCTTCAATGGCAAGCTTCCTCGCACCTGGTTCAGAACCGCTCATCAGCTGCCTATGAATGTTGGAGATTCACATTATGATCCTCTCTCTTCCCTTCTGGATATGGCCTCACTACACAACCCACCAATGCCAACTAGGAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005623 GO:0005886 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0008422 GO:0009056 GO:0009057 GO:0009251 GO:0015926 GO:0016020 GO:0016052 GO:0016787 GO:0016798 GO:0042597 GO:0043170 GO:0044042 GO:0044238 GO:0044464 GO:0071704 GO:0071944 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00460 ko00500 ko00940 ko01100 ko01110 map00460 map00500 map00940 map01100 map01110

Pfam Domains

Protein Families

Glyco_hydro_3 Glyco_hydro_3_C

Protein Analysis

548

Amino Acids

59.88

Weight (kDa)

8.92

Isoelectric Point (pI)

31.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_3	PF00933	51 - 277	1.8e-43	Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C	PF01915	347 - 521	4.8e-29	Glycosyl hydrolase family 3 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000924)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04885 AT5G04885 AT5G04885
fragaria_vesca	FvH4_1g28352 FvH4_1g28352 FvH4_1g28352 FvH4_4g26180 FvH4_4g26180 FvH4_7g05220 FvH4_7g05220
malus_domestica	MD02G1259600.v1.1 MD07G1060900.v1.1 MD11G1177800.v1.1 MD11G1177900.v1.1 MD14G1004200.v1.1
prunus_persica	Prupe.2G065700_v2.0.a1 Prupe.2G065700_v2.0.a1 Prupe.2G065700_v2.0.a1 Prupe.2G065700_v2.0.a1 Prupe.2G065700_v2.0.a1 Prupe.6G150000_v2.0.a1 Prupe.6G150200_v2.0.a1 Prupe.6G150400_v2.0.a1 Prupe.6G150500_v2.0.a1 Prupe.7G007200_v2.0.a1
pyrus_communis	pycom14g00250
rosa_chinensis	RchiOBHm_Chr1g0331601 RchiOBHm_Chr3g0496681 RchiOBHm_Chr3g0496721 RchiOBHm_Chr4g0433771
rosa_laevigata	RLG00000006732 RLG00000022510 RLG00000022511 RLG00000029727 RLG00000034619
rosa_multiflora	Rmu_sc0005044.1_g000017 Rmu_sc0006176.1_g000006
rosa_roxburghii	Rroxscaffold_4G00319030 Rroxscaffold_5G00375200 Rroxscaffold_6G00388790 Rroxscaffold_6G00388830 Rroxscaffold_6G00388850
rosa_rugosa	Rorug01G0095000 Rorug01G0095100 Rorug01G0095200 Rorug03G0185800 Rorug03G0186000 Rorug03G0285500 Rorug03G0285700 Rorug04G0271300 Rorug05G0248600 Rorug06G0034700
rosa_samantha	Rh1AG118800 Rh1CG113800 Rh3AG330600 Rh3BG366600 Rh3CG363300 Rh3CG363500 Rh4AG326000 Rh4CG349100 Rh5AG325000
rosa_wichuraiana	Rw1G009560 Rw3G028940 Rw3G028950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	1116
AccBSI	CCGCTC	1 cut(s)	1544
AccI	GTMKAC	1 cut(s)	390
AccII	CGCG	1 cut(s)	476
AciI	CCGC	6 cut(s)	190, 474, 527, 967, 1359, 1542
AclWI	GGATC	4 cut(s)	533, 572, 903, 1577
AcoI	YGGCCR	4 cut(s)	169, 376, 1333, 1393
AcsI	RAATTY	4 cut(s)	9, 884, 960, 1053
AcuI	CTGAAG	1 cut(s)	1485
AdeI	CACNNNGTG	1 cut(s)	521
AfaI	GTAC	3 cut(s)	218, 224, 1208
AfiI	CCNNNNNNNGG	3 cut(s)	548, 1116, 1331
AgsI	TTSAA	4 cut(s)	314, 482, 593, 1510
AjnI	CCWGG	4 cut(s)	434, 613, 1456, 1529
Alw21I	GWGCWC	1 cut(s)	658
Alw26I	GTCTC	1 cut(s)	1491
AlwI	GGATC	4 cut(s)	533, 572, 903, 1577
AlwNI	CAGNNNCTG	1 cut(s)	1463
AoxI	GGCC	7 cut(s)	169, 376, 730, 1333, 1393, 1468, 1608
ApeKI	GCWGC	6 cut(s)	467, 686, 1030, 1364, 1445, 1552
ApoI	RAATTY	4 cut(s)	9, 884, 960, 1053
AspLEI	GCGC	2 cut(s)	105, 478
AspS9I	GGNCC	3 cut(s)	439, 730, 1107
AsuHPI	GGTGA	5 cut(s)	195, 223, 313, 1313, 1381
AvaII	GGWCC	2 cut(s)	439, 1107
AxyI	CCTNAGG	1 cut(s)	207
BaeI	ACNNNNGTAYC	2 cut(s)	1425, 1458
BalI	TGGCCA	3 cut(s)	171, 378, 1335
BauI	CACGAG	1 cut(s)	1146
Bbv12I	GWGCWC	1 cut(s)	658
BbvI	GCAGC	6 cut(s)	454, 698, 1017, 1376, 1457, 1539
BccI	CCATC	4 cut(s)	540, 707, 1268, 1433
BceAI	ACGGC	1 cut(s)	1380
BcgI	CGANNNNNNTGC	2 cut(s)	665, 699
BciT130I	CCWGG	4 cut(s)	436, 615, 1458, 1531
BclI	TGATCA	1 cut(s)	1407
BcoDI	GTCTC	1 cut(s)	1491
BfaI	CTAG	4 cut(s)	6, 123, 645, 1638
BfmI	CTRYAG	4 cut(s)	279, 492, 568, 1401
BisI	GCNGC	6 cut(s)	468, 687, 1031, 1365, 1446, 1553
BlsI	GCNGC	6 cut(s)	469, 688, 1032, 1366, 1447, 1554
Bme1390I	CCNGG	4 cut(s)	436, 615, 1458, 1531
Bme18I	GGWCC	2 cut(s)	439, 1107
BmgT120I	GGNCC	3 cut(s)	439, 730, 1107
BmiI	GGNNCC	4 cut(s)	440, 441, 867, 1454
BmrFI	CCNGG	4 cut(s)	436, 615, 1458, 1531
BmrI	ACTGGG	1 cut(s)	434
BmsI	GCATC	3 cut(s)	355, 937, 1213
BmuI	ACTGGG	1 cut(s)	434
BpmI	CTGGAG	1 cut(s)	781
Bpu10I	CCTNAGC	1 cut(s)	582
BsaBI	GATNNNNATC	1 cut(s)	325
BsaJI	CCNNGG	6 cut(s)	153, 435, 1290, 1456, 1457, 1471
BsaXI	ACNNNNNCTCC	2 cut(s)	241, 271
Bsc4I	CCNNNNNNNGG	3 cut(s)	548, 1116, 1331
Bse118I	RCCGGY	1 cut(s)	1391
Bse1I	ACTGG	2 cut(s)	429, 798
Bse21I	CCTNAGG	1 cut(s)	207
Bse3DI	GCAATG	1 cut(s)	603
Bse8I	GATNNNNATC	1 cut(s)	325
BseBI	CCWGG	4 cut(s)	436, 615, 1458, 1531
BseDI	CCNNGG	6 cut(s)	153, 435, 1290, 1456, 1457, 1471
BseGI	GGATG	3 cut(s)	153, 326, 924
BseJI	GATNNNNATC	1 cut(s)	325
BseLI	CCNNNNNNNGG	3 cut(s)	548, 1116, 1331
BseMI	GCAATG	1 cut(s)	603
BseMII	CTCAG	5 cut(s)	131, 198, 594, 764, 1027
BseNI	ACTGG	2 cut(s)	429, 798
BseX3I	CGGCCG	1 cut(s)	1393
BseXI	GCAGC	6 cut(s)	454, 698, 1017, 1376, 1457, 1539
BsgI	GTGCAG	1 cut(s)	1383
Bsh1236I	CGCG	1 cut(s)	476
Bsh1285I	CGRYCG	1 cut(s)	1396
BshFI	GGCC	7 cut(s)	171, 378, 732, 1335, 1395, 1470, 1610
BsiEI	CGRYCG	1 cut(s)	1396
BsiHKAI	GWGCWC	1 cut(s)	658
BsiSI	CCGG	2 cut(s)	145, 1392
BslFI	GGGAC	3 cut(s)	452, 1137, 1474
BslI	CCNNNNNNNGG	3 cut(s)	548, 1116, 1331
BsmAI	GTCTC	1 cut(s)	1491
BsmFI	GGGAC	3 cut(s)	452, 1137, 1474
BsmI	GAATGC	2 cut(s)	89, 777
BsnI	GGCC	7 cut(s)	171, 378, 732, 1335, 1395, 1470, 1610
Bsp1286I	GDGCHC	1 cut(s)	658
Bsp1407I	TGTACA	2 cut(s)	216, 1206
Bsp143I	GATC	7 cut(s)	538, 577, 606, 895, 1009, 1407, 1582
BspACI	CCGC	6 cut(s)	190, 474, 527, 967, 1359, 1542
BspANI	GGCC	7 cut(s)	171, 378, 732, 1335, 1395, 1470, 1610
BspCNI	CTCAG	5 cut(s)	132, 199, 595, 765, 1026
BspFNI	CGCG	1 cut(s)	476
BspLI	GGNNCC	4 cut(s)	440, 441, 867, 1454
BspMAI	CTGCAG	1 cut(s)	283
BspPI	GGATC	4 cut(s)	533, 572, 903, 1577
BsrBI	CCGCTC	1 cut(s)	1544
BsrDI	GCAATG	1 cut(s)	603
BsrFI	RCCGGY	1 cut(s)	1391
BsrGI	TGTACA	2 cut(s)	216, 1206
BsrI	ACTGG	2 cut(s)	429, 798
BssAI	RCCGGY	1 cut(s)	1391
BssECI	CCNNGG	6 cut(s)	153, 435, 1290, 1456, 1457, 1471
BssMI	GATC	7 cut(s)	538, 577, 606, 895, 1009, 1407, 1582
BssSI	CACGAG	1 cut(s)	1146
BssT1I	CCWWGG	2 cut(s)	153, 1471
Bst2BI	CACGAG	1 cut(s)	1146
Bst2UI	CCWGG	4 cut(s)	436, 615, 1458, 1531
Bst4CI	ACNGT	4 cut(s)	572, 751, 1002, 1183
Bst6I	CTCTTC	2 cut(s)	950, 1597
BstAPI	GCANNNNNTGC	1 cut(s)	692
BstAUI	TGTACA	2 cut(s)	216, 1206
BstC8I	GCNNGC	4 cut(s)	264, 339, 844, 1517
BstDEI	CTNAG	7 cut(s)	140, 207, 582, 603, 773, 1013, 1127
BstF5I	GGATG	3 cut(s)	153, 326, 924
BstFNI	CGCG	1 cut(s)	476
BstHHI	GCGC	2 cut(s)	105, 478
BstKTI	GATC	7 cut(s)	541, 580, 609, 898, 1012, 1410, 1585
BstMAI	GTCTC	1 cut(s)	1491
BstMBI	GATC	7 cut(s)	538, 577, 606, 895, 1009, 1407, 1582
BstMCI	CGRYCG	1 cut(s)	1396
BstMWI	GCNNNNNNNGC	5 cut(s)	272, 473, 692, 1295, 1512
BstNI	CCWGG	4 cut(s)	436, 615, 1458, 1531
BstNSI	RCATGY	1 cut(s)	787
BstSCI	CCNGG	4 cut(s)	434, 613, 1456, 1529
BstSFI	CTRYAG	4 cut(s)	279, 492, 568, 1401
BstUI	CGCG	1 cut(s)	476
BstV1I	GCAGC	6 cut(s)	454, 698, 1017, 1376, 1457, 1539
BstX2I	RGATCY	3 cut(s)	538, 577, 895
BstXI	CCANNNNNNTGG	2 cut(s)	1034, 1267
BstYI	RGATCY	3 cut(s)	538, 577, 895
BstZI	CGGCCG	1 cut(s)	1393
Bsu36I	CCTNAGG	1 cut(s)	207
BsuRI	GGCC	7 cut(s)	171, 378, 732, 1335, 1395, 1470, 1610
BtsCI	GGATG	3 cut(s)	153, 326, 924
BtsIMutI	CAGTG	6 cut(s)	347, 577, 747, 805, 844, 1100
Cac8I	GCNNGC	4 cut(s)	264, 339, 844, 1517
CaiI	CAGNNNCTG	1 cut(s)	1463
CfoI	GCGC	2 cut(s)	105, 478
Cfr10I	RCCGGY	1 cut(s)	1391
Cfr13I	GGNCC	3 cut(s)	439, 730, 1107
CseI	GACGC	1 cut(s)	187
CsiI	ACCWGGT	1 cut(s)	1529
Csp6I	GTAC	3 cut(s)	217, 223, 1207
CviQI	GTAC	3 cut(s)	217, 223, 1207
DdeI	CTNAG	7 cut(s)	140, 207, 582, 603, 773, 1013, 1127
DpnI	GATC	7 cut(s)	540, 579, 608, 897, 1011, 1409, 1584
DpnII	GATC	7 cut(s)	538, 577, 606, 895, 1009, 1407, 1582
DraIII	CACNNNGTG	1 cut(s)	521
EaeI	YGGCCR	4 cut(s)	169, 376, 1333, 1393
EagI	CGGCCG	1 cut(s)	1393
Eam1104I	CTCTTC	2 cut(s)	950, 1597
EarI	CTCTTC	2 cut(s)	950, 1597
EclXI	CGGCCG	1 cut(s)	1393
Eco130I	CCWWGG	2 cut(s)	153, 1471
Eco32I	GATATC	1 cut(s)	633
Eco47I	GGWCC	2 cut(s)	439, 1107
Eco52I	CGGCCG	1 cut(s)	1393
Eco57I	CTGAAG	1 cut(s)	1485
Eco81I	CCTNAGG	1 cut(s)	207
EcoO109I	RGGNCCY	1 cut(s)	439
EcoRI	GAATTC	2 cut(s)	9, 884
EcoRII	CCWGG	4 cut(s)	434, 613, 1456, 1529
EcoRV	GATATC	1 cut(s)	633
EcoT14I	CCWWGG	2 cut(s)	153, 1471
EcoT22I	ATGCAT	2 cut(s)	53, 89
ErhI	CCWWGG	2 cut(s)	153, 1471
FaqI	GGGAC	3 cut(s)	452, 1137, 1474
FauI	CCCGC	1 cut(s)	960
FbaI	TGATCA	1 cut(s)	1407
FblI	GTMKAC	1 cut(s)	390
Fnu4HI	GCNGC	6 cut(s)	468, 687, 1031, 1365, 1446, 1553
FokI	GGATG	3 cut(s)	160, 333, 911
Fsp4HI	GCNGC	6 cut(s)	468, 687, 1031, 1365, 1446, 1553
FspBI	CTAG	4 cut(s)	6, 123, 645, 1638
GlaI	GCGC	2 cut(s)	104, 477
GluI	GCNGC	6 cut(s)	468, 687, 1031, 1365, 1446, 1553
GsuI	CTGGAG	1 cut(s)	781
HaeIII	GGCC	7 cut(s)	171, 378, 732, 1335, 1395, 1470, 1610
HapII	CCGG	2 cut(s)	145, 1392
HgaI	GACGC	1 cut(s)	187
HhaI	GCGC	2 cut(s)	105, 478
Hin6I	GCGC	2 cut(s)	103, 476
HinP1I	GCGC	2 cut(s)	103, 476
HincII	GTYRAC	1 cut(s)	1024
HindII	GTYRAC	1 cut(s)	1024
HindIII	AAGCTT	2 cut(s)	584, 1517
HinfI	GANTC	7 cut(s)	126, 331, 498, 834, 1216, 1341, 1571
HpaII	CCGG	2 cut(s)	145, 1392
HphI	GGTGA	5 cut(s)	195, 223, 313, 1313, 1381
Hpy166II	GTNNAC	5 cut(s)	391, 770, 978, 1024, 1207
Hpy188I	TCNGA	4 cut(s)	1016, 1044, 1349, 1538
Hpy188III	TCNNGA	3 cut(s)	645, 1220, 1601
Hpy8I	GTNNAC	5 cut(s)	391, 770, 978, 1024, 1207
Hpy99I	CGWCG	1 cut(s)	70
HpyAV	CCTTC	3 cut(s)	64, 1460, 1607
HpyCH4III	ACNGT	4 cut(s)	572, 751, 1002, 1183
HpyF10VI	GCNNNNNNNGC	5 cut(s)	272, 473, 692, 1295, 1512
HpyF3I	CTNAG	7 cut(s)	140, 207, 582, 603, 773, 1013, 1127
HspAI	GCGC	2 cut(s)	103, 476
KflI	GGGWCCC	1 cut(s)	439
Ksp22I	TGATCA	1 cut(s)	1407
Kzo9I	GATC	7 cut(s)	538, 577, 606, 895, 1009, 1407, 1582
LmnI	GCTCC	8 cut(s)	28, 100, 428, 464, 661, 800, 1295, 1458
Lsp1109I	GCAGC	6 cut(s)	454, 698, 1017, 1376, 1457, 1539
LweI	GCATC	3 cut(s)	355, 937, 1213
MabI	ACCWGGT	1 cut(s)	1529
MaeI	CTAG	4 cut(s)	6, 123, 645, 1638
MaeIII	GTNAC	2 cut(s)	557, 1301
MalI	GATC	7 cut(s)	540, 579, 608, 897, 1011, 1409, 1584
MbiI	CCGCTC	1 cut(s)	1544
MboI	GATC	7 cut(s)	538, 577, 606, 895, 1009, 1407, 1582
MboII	GAAGA	5 cut(s)	10, 13, 587, 967, 1584
MflI	RGATCY	3 cut(s)	538, 577, 895
MhlI	GDGCHC	1 cut(s)	658
MlsI	TGGCCA	3 cut(s)	171, 378, 1335
MluCI	AATT	7 cut(s)	9, 804, 884, 939, 960, 1053, 1158
MluNI	TGGCCA	3 cut(s)	171, 378, 1335
MlyI	GAGTC	2 cut(s)	325, 1350
MmeI	TCCRAC	2 cut(s)	1085, 1546
Mox20I	TGGCCA	3 cut(s)	171, 378, 1335
Mph1103I	ATGCAT	2 cut(s)	53, 89
MscI	TGGCCA	3 cut(s)	171, 378, 1335
MseI	TTAA	4 cut(s)	116, 453, 972, 1316
MslI	CAYNNNNRTG	5 cut(s)	384, 782, 851, 929, 1265
Msp20I	TGGCCA	3 cut(s)	171, 378, 1335
MspA1I	CMGCKG	2 cut(s)	1030, 1552
MspI	CCGG	2 cut(s)	145, 1392
MspR9I	CCNGG	4 cut(s)	436, 615, 1458, 1531
Mva1269I	GAATGC	2 cut(s)	89, 777
MvaI	CCWGG	4 cut(s)	436, 615, 1458, 1531
MvnI	CGCG	1 cut(s)	476
MwoI	GCNNNNNNNGC	5 cut(s)	272, 473, 692, 1295, 1512
NdeII	GATC	7 cut(s)	538, 577, 606, 895, 1009, 1407, 1582
NlaIV	GGNNCC	4 cut(s)	440, 441, 867, 1454
NmeAIII	GCCGAG	2 cut(s)	313, 1315
NmuCI	GTSAC	1 cut(s)	1301
NsiI	ATGCAT	2 cut(s)	53, 89
NspI	RCATGY	1 cut(s)	787
PasI	CCCWGGG	1 cut(s)	1457
PctI	GAATGC	2 cut(s)	89, 777
PfeI	GAWTC	5 cut(s)	126, 498, 834, 1216, 1571
PflMI	CCANNNNNTGG	1 cut(s)	1116
PkrI	GCNGC	6 cut(s)	469, 688, 1032, 1366, 1447, 1554
PleI	GAGTC	2 cut(s)	325, 1349
PpsI	GAGTC	2 cut(s)	325, 1349
PpuMI	RGGWCCY	1 cut(s)	439
Psp5II	RGGWCCY	1 cut(s)	439
Psp6I	CCWGG	4 cut(s)	434, 613, 1456, 1529
PspGI	CCWGG	4 cut(s)	434, 613, 1456, 1529
PspN4I	GGNNCC	4 cut(s)	440, 441, 867, 1454
PspPI	GGNCC	3 cut(s)	439, 730, 1107
PspPPI	RGGWCCY	1 cut(s)	439
PstI	CTGCAG	1 cut(s)	283
PstNI	CAGNNNCTG	1 cut(s)	1463
PsuI	RGATCY	3 cut(s)	538, 577, 895
PvuII	CAGCTG	2 cut(s)	1030, 1552
RsaI	GTAC	3 cut(s)	218, 224, 1208
RsaNI	GTAC	3 cut(s)	217, 223, 1207
RseI	CAYNNNNRTG	5 cut(s)	384, 782, 851, 929, 1265
SaqAI	TTAA	4 cut(s)	116, 453, 972, 1316
SatI	GCNGC	6 cut(s)	468, 687, 1031, 1365, 1446, 1553
Sau3AI	GATC	7 cut(s)	538, 577, 606, 895, 1009, 1407, 1582
Sau96I	GGNCC	3 cut(s)	439, 730, 1107
SchI	GAGTC	2 cut(s)	325, 1350
ScrFI	CCNGG	4 cut(s)	436, 615, 1458, 1531
SduI	GDGCHC	1 cut(s)	658
SexAI	ACCWGGT	1 cut(s)	1529
SfaNI	GCATC	3 cut(s)	355, 937, 1213
SfcI	CTRYAG	4 cut(s)	279, 492, 568, 1401
SinI	GGWCC	2 cut(s)	439, 1107
SmiMI	CAYNNNNRTG	5 cut(s)	384, 782, 851, 929, 1265
Sse9I	AATT	7 cut(s)	9, 804, 884, 939, 960, 1053, 1158
SsiI	CCGC	6 cut(s)	190, 474, 527, 967, 1359, 1542
SspI	AATATT	1 cut(s)	418
SspMI	CTAG	4 cut(s)	6, 123, 645, 1638
StyD4I	CCNGG	4 cut(s)	434, 613, 1456, 1529
StyI	CCWWGG	2 cut(s)	153, 1471
TaaI	ACNGT	4 cut(s)	572, 751, 1002, 1183
TaqI	TCGA	1 cut(s)	329
TasI	AATT	7 cut(s)	9, 804, 884, 939, 960, 1053, 1158
TatI	WGTACW	3 cut(s)	216, 222, 1206
TfiI	GAWTC	5 cut(s)	126, 498, 834, 1216, 1571
Tru1I	TTAA	4 cut(s)	116, 453, 972, 1316
Tru9I	TTAA	4 cut(s)	116, 453, 972, 1316
TscAI	CASTG	6 cut(s)	354, 577, 754, 805, 844, 1100
TseFI	GTSAC	1 cut(s)	1301
TseI	GCWGC	6 cut(s)	467, 686, 1030, 1364, 1445, 1552
Tsp45I	GTSAC	1 cut(s)	1301
TspDTI	ATGAA	4 cut(s)	13, 362, 877, 1574
TspGWI	ACGGA	1 cut(s)	333
TspRI	CASTG	6 cut(s)	354, 577, 754, 805, 844, 1100
Van91I	CCANNNNNTGG	1 cut(s)	1116
VpaK11BI	GGWCC	2 cut(s)	439, 1107
XapI	RAATTY	4 cut(s)	9, 884, 960, 1053
XbaI	TCTAGA	1 cut(s)	644
XceI	RCATGY	1 cut(s)	787
XmiI	GTMKAC	1 cut(s)	390
XspI	CTAG	4 cut(s)	6, 123, 645, 1638
Zsp2I	ATGCAT	2 cut(s)	53, 89

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.