Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0000924

Physical Location & Seq

Genomic Coordinates Forward (+)

31289983 .. 31296111

Gene Structure 6129 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1259600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2064 bp

ATGGTTAGAACAAATGATGGATATTGTGTAATTCTGGTTTTTCGTGTTTGGTTTACCAGAGTGAAAAAGGACCCATTTACAATGGCTTGGGTTTCGGTACCAGTCGTGGGTTTGTTTTTGGTTTGGTGGTGGATGGGTTTGGCTACGGTCAAGGCTGATTACATCATGTACAAAGATCCAACCCAACCAGCGGCTGCTCGAGTTGGGGACCTTCTTAGCAGGATGACTCTTGAAGAGAAGATTGGTCAGATGGTGCAGATTGATAGGAGTGTTGCCTCTATTGATACTATGAAAACTTACTTCATAGGTAGTGTATTGAGCGGTGGTGGCAGTACACCACTTCCAGAAGCTAGTGCTGAAGACTGGGTTAACATGATTAATGGATTTCAGAAGGGAGCTCTATCTAGTCGTTTGGGCATTCCTATGATCTATGGCATTGATGCTGTTCATGGACACAATAATGTCTACAATGCTACAATATTTCCGCATAATGTTGGGCTTGGAGCTACCAGGGACCCTGAACTGGTACGAAGAATTGGTTCTGCAACTGCTCTTGAAGCCCGAGCTACGGGGATTCCTTATGTATTTGCTCCTTGCATTGCGGTATGCAGAGATCCAAGATGGGGCCGGTGTTACGAAAGTTACAGCGAAGATCACACAATTGTGCAAGAAATGACGGAGATTATTCCAGGTTTACAGGGAGATATCCCTGCAAATTCTCAGAAAGGAGTGCCATATGTTGGGGGAAATAAAAAGGTTGCAGCTTGTGCAAAGCACTTCGTTGGTGATGGTGGCACAAGCAAGGGCATTAATGAGTACAACACTGTGGTTGACCAACAGGAATTGCTTAGCATCCACATGCCAGCCTATTCTGACTCCATCATCAAGGGTGTCTCAACCGTGATGATTTCCTACTCCAGTTGGAATGGGAAAAAGATGCATGCAAACCGTGATCTAATTACGGACTACCTCAAGGGCACCCTTCAATTTAAGGGTTTTGTTATATCAGACTGGGAGGGTATTGACAGGATTACCTCACCGCCACATTCAAACTACACATACTCTGTCCAAGATTCAGTTCTAGCTGGAATTGACATGGTCATGGTCCCTATCAACTATAAAGAATTCATTAATGATCTCATTAACCTGGTCAAGAATAACGTTGTCCCAATGGATCGTATTGATGATGCTGCGGGAAGGATTCTGCTTGTCAAGTTCACCATGGGTCTTTTTGAGAACCCGTTGGCTGATTTCAGCCTAGTCAATGAGCTTGGAAGCCAGGATCATAGAGATTTGGCAAGGGAGGCTGTCAGAAAATCCGTCGTGCTTTTGAAGAATGGGAAAAACGGGAACATTACAGATCCAGTCATACCTCTTCCCAAGAAGGTGTCTAAAATTCTAGTTGCCGGCAGTCATGCTGATAATCTGGGCTACCAATGTGGTGGATGGACAATTGCATGGCAAGGATTCAGTGGCAACAAATATACATCCGGGACTACGATCCTTGGTGCTATAAAATCAGCAGTCGACTCAAGCACAGAAGTTATCTACAGTGAGAATCCTGATGGTAATTTTGTGAAGTCCAACAACTTTTCATATGCCATTGTTGTGGTTGGCGAGCATCCTTATGCTGAGACTGCGGGAGACAGCCCAAACCTAATGATGGCGGAACCTGGCCCAAGTGTCATCAGCAATGTGTGTGAAAGTGTCAAGTGCATTGTTGTCTTAATAACTGGCAGACCTATTGTAATTGAACCATATATTTCCTCGATTGATGCTCTGGTGGCAGCATGGTTGCCAGGCTCTGAAGGCCAAGGCATTACTGATGTCCTTTTTGGGGATCGTGGATTCAGTGGAAAGCTTCCAAGAACATGGTTCAGAACTGTCGATCAACTCCCAATGAATGTTGGTGATTCACACTATGATCCACTTTTCCCGTTTGGGTTTGGACTGGAAACTGAGTCAGTAAAGGAGCTTGTAACAAGGTCGACCTCAGCTGGTGTTGTTGCAAGGCCATGCATACTTATCGTCCTGGTTGCTCTGATTCTTAGCTTTATAGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0000323 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005886 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0008422 GO:0009056 GO:0009057 GO:0009251 GO:0015926 GO:0016020 GO:0016052 GO:0016787 GO:0016798 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0071704 GO:0071944 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00460 ko00500 ko00940 ko01100 ko01110 map00460 map00500 map00940 map01100 map01110

Pfam Domains

Protein Families

Glyco_hydro_3 Glyco_hydro_3_C

Protein Analysis

688

Amino Acids

74.94

Weight (kDa)

5.81

Isoelectric Point (pI)

28.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_3	PF00933	76 - 402	4.1e-66	Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C	PF01915	441 - 652	4.3e-37	Glycosyl hydrolase family 3 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000924)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04885 AT5G04885 AT5G04885
fragaria_vesca	FvH4_1g28352 FvH4_1g28352 FvH4_1g28352 FvH4_4g26180 FvH4_4g26180 FvH4_7g05220 FvH4_7g05220
malus_domestica	MD02G1259600.v1.1 MD07G1060900.v1.1 MD11G1177800.v1.1 MD11G1177900.v1.1 MD14G1004200.v1.1
prunus_persica	Prupe.2G065700_v2.0.a1 Prupe.2G065700_v2.0.a1 Prupe.2G065700_v2.0.a1 Prupe.2G065700_v2.0.a1 Prupe.2G065700_v2.0.a1 Prupe.6G150000_v2.0.a1 Prupe.6G150200_v2.0.a1 Prupe.6G150400_v2.0.a1 Prupe.6G150500_v2.0.a1 Prupe.7G007200_v2.0.a1
pyrus_communis	pycom14g00250
rosa_chinensis	RchiOBHm_Chr1g0331601 RchiOBHm_Chr3g0496681 RchiOBHm_Chr3g0496721 RchiOBHm_Chr4g0433771
rosa_laevigata	RLG00000006732 RLG00000022510 RLG00000022511 RLG00000029727 RLG00000034619
rosa_multiflora	Rmu_sc0005044.1_g000017 Rmu_sc0006176.1_g000006
rosa_roxburghii	Rroxscaffold_4G00319030 Rroxscaffold_5G00375200 Rroxscaffold_6G00388790 Rroxscaffold_6G00388830 Rroxscaffold_6G00388850
rosa_rugosa	Rorug01G0095000 Rorug01G0095100 Rorug01G0095200 Rorug03G0185800 Rorug03G0186000 Rorug03G0285500 Rorug03G0285700 Rorug04G0271300 Rorug05G0248600 Rorug06G0034700
rosa_samantha	Rh1AG118800 Rh1CG113800 Rh3AG330600 Rh3BG366600 Rh3CG363300 Rh3CG363500 Rh4AG326000 Rh4CG349100 Rh5AG325000
rosa_wichuraiana	Rw1G009560 Rw3G028940 Rw3G028950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	97
AccB1I	GGYRCC	2 cut(s)	97, 977
AccB7I	CCANNNNNTGG	1 cut(s)	740
AccBSI	CCGCTC	1 cut(s)	321
AccI	GTMKAC	3 cut(s)	465, 1527, 1988
AciI	CCGC	8 cut(s)	191, 321, 485, 602, 1040, 1193, 1640, 1667
AclI	AACGTT	1 cut(s)	1161
AclWI	GGATC	8 cut(s)	170, 608, 1182, 1290, 1355, 1495, 1848, 1919
AcsI	RAATTY	3 cut(s)	715, 1124, 1395
AcuI	CTGAAG	2 cut(s)	378, 1827
AfaI	GTAC	5 cut(s)	99, 170, 334, 528, 818
AfiI	CCNNNNNNNGG	8 cut(s)	107, 190, 523, 568, 623, 740, 1663, 1837
AgsI	TTSAA	6 cut(s)	233, 557, 986, 1050, 1333, 1754
AjnI	CCWGG	7 cut(s)	509, 688, 1146, 1278, 1672, 1798, 2031
AjuI	GAANNNNNNNTTGG	4 cut(s)	225, 257, 1062, 1094
AleI	CACNNNNGTG	1 cut(s)	662
Alw21I	GWGCWC	1 cut(s)	400
Alw26I	GTCTC	3 cut(s)	898, 1628, 1638
AlwI	GGATC	8 cut(s)	170, 608, 1182, 1290, 1355, 1495, 1848, 1919
AlwNI	CAGNNNCTG	2 cut(s)	194, 1805
Ama87I	CYCGRG	2 cut(s)	198, 561
AoxI	GGCC	4 cut(s)	625, 1675, 1810, 2012
ApeKI	GCWGC	4 cut(s)	194, 761, 1190, 1787
ApoI	RAATTY	3 cut(s)	715, 1124, 1395
AseI	ATTAAT	3 cut(s)	378, 810, 1131
Asp700I	GAANNNNTTC	1 cut(s)	538
Asp718I	GGTACC	1 cut(s)	97
AspS9I	GGNCC	6 cut(s)	70, 208, 514, 625, 1105, 1676
AsuC2I	CCSGG	1 cut(s)	1492
AsuHPI	GGTGA	4 cut(s)	797, 1029, 1210, 1922
AvaI	CYCGRG	2 cut(s)	198, 561
AvaII	GGWCC	4 cut(s)	70, 208, 514, 1105
BaeGI	GKGCMC	1 cut(s)	980
BanI	GGYRCC	2 cut(s)	97, 977
BanII	GRGCYC	1 cut(s)	400
BbsI	GAAGAC	1 cut(s)	366
Bbv12I	GWGCWC	1 cut(s)	400
BbvCI	CCTCAGC	1 cut(s)	1993
BbvI	GCAGC	4 cut(s)	181, 773, 1177, 1799
BccI	CCATC	9 cut(s)	11, 127, 244, 615, 782, 887, 1440, 1559, 1657
BcgI	CGANNNNNNTGC	2 cut(s)	2008, 2042
BciT130I	CCWGG	7 cut(s)	511, 690, 1148, 1280, 1674, 1800, 2033
BcnI	CCSGG	1 cut(s)	1492
BcoDI	GTCTC	3 cut(s)	898, 1628, 1638
BfaI	CTAG	5 cut(s)	351, 405, 1082, 1259, 1400
BfmI	CTRYAG	1 cut(s)	1549
BisI	GCNGC	5 cut(s)	192, 195, 762, 1191, 1788
BlpI	GCTNAGC	1 cut(s)	848
BlsI	GCNGC	5 cut(s)	193, 196, 763, 1192, 1789
Bme1390I	CCNGG	8 cut(s)	511, 690, 1148, 1280, 1492, 1674, 1800, 2033
Bme18I	GGWCC	4 cut(s)	70, 208, 514, 1105
BmeT110I	CYCGRG	2 cut(s)	198, 561
BmgT120I	GGNCC	6 cut(s)	70, 208, 514, 625, 1105, 1676
BmiI	GGNNCC	9 cut(s)	72, 99, 209, 515, 516, 626, 979, 1107, 1671
BmrFI	CCNGG	8 cut(s)	511, 690, 1148, 1280, 1492, 1674, 1800, 2033
BmrI	ACTGGG	2 cut(s)	373, 1021
BmsI	GCATC	6 cut(s)	430, 861, 927, 1177, 1630, 1765
BmuI	ACTGGG	2 cut(s)	373, 1021
BpiI	GAAGAC	1 cut(s)	366
BpmI	CTGGAG	1 cut(s)	901
Bpu10I	CCTNAGC	1 cut(s)	1993
Bpu1102I	GCTNAGC	1 cut(s)	848
BpuEI	CTTGAG	2 cut(s)	956, 1516
BpuMI	CCSGG	1 cut(s)	1492
BsaJI	CCNNGG	4 cut(s)	510, 1221, 1504, 1813
Bsc4I	CCNNNNNNNGG	8 cut(s)	107, 190, 523, 568, 623, 740, 1663, 1837
Bse118I	RCCGGY	2 cut(s)	627, 1406
Bse1I	ACTGG	8 cut(s)	101, 368, 528, 918, 1016, 1364, 1738, 1956
Bse3DI	GCAATG	2 cut(s)	597, 1699
BseBI	CCWGG	7 cut(s)	511, 690, 1148, 1280, 1674, 1800, 2033
BseDI	CCNNGG	4 cut(s)	510, 1221, 1504, 1813
BseGI	GGATG	6 cut(s)	138, 228, 852, 1451, 1487, 1621
BseLI	CCNNNNNNNGG	8 cut(s)	107, 190, 523, 568, 623, 740, 1663, 1837
BseMI	GCAATG	2 cut(s)	597, 1699
BseMII	CTCAG	4 cut(s)	734, 1623, 1950, 2007
BseNI	ACTGG	8 cut(s)	101, 368, 528, 918, 1016, 1364, 1738, 1956
BseSI	GKGCMC	1 cut(s)	980
BseXI	GCAGC	4 cut(s)	181, 773, 1177, 1799
BsgI	GTGCAG	1 cut(s)	275
BshFI	GGCC	4 cut(s)	627, 1677, 1812, 2014
BshNI	GGYRCC	2 cut(s)	97, 977
BsiHKAI	GWGCWC	1 cut(s)	400
BsiHKCI	CYCGRG	2 cut(s)	198, 561
BsiSI	CCGG	3 cut(s)	628, 1407, 1491
BslFI	GGGAC	5 cut(s)	221, 527, 1091, 1151, 1507
BslI	CCNNNNNNNGG	8 cut(s)	107, 190, 523, 568, 623, 740, 1663, 1837
BsmAI	GTCTC	3 cut(s)	898, 1628, 1638
BsmFI	GGGAC	5 cut(s)	221, 527, 1091, 1151, 1507
BsmI	GAATGC	1 cut(s)	417
BsnI	GGCC	4 cut(s)	627, 1677, 1812, 2014
BsoBI	CYCGRG	2 cut(s)	198, 561
Bsp1286I	GDGCHC	2 cut(s)	400, 980
Bsp1407I	TGTACA	1 cut(s)	168
Bsp1720I	GCTNAGC	1 cut(s)	848
Bsp19I	CCATGG	1 cut(s)	1221
BspACI	CCGC	8 cut(s)	191, 321, 485, 602, 1040, 1193, 1640, 1667
BspANI	GGCC	4 cut(s)	627, 1677, 1812, 2014
BspCNI	CTCAG	4 cut(s)	733, 1624, 1951, 2006
BspLI	GGNNCC	9 cut(s)	72, 99, 209, 515, 516, 626, 979, 1107, 1671
BspPI	GGATC	8 cut(s)	170, 608, 1182, 1290, 1355, 1495, 1848, 1919
BspT107I	GGYRCC	2 cut(s)	97, 977
BsrBI	CCGCTC	1 cut(s)	321
BsrDI	GCAATG	2 cut(s)	597, 1699
BsrFI	RCCGGY	2 cut(s)	627, 1406
BsrGI	TGTACA	1 cut(s)	168
BsrI	ACTGG	8 cut(s)	101, 368, 528, 918, 1016, 1364, 1738, 1956
BssAI	RCCGGY	2 cut(s)	627, 1406
BssECI	CCNNGG	4 cut(s)	510, 1221, 1504, 1813
BssT1I	CCWWGG	3 cut(s)	1221, 1504, 1813
Bst2UI	CCWGG	7 cut(s)	511, 690, 1148, 1280, 1674, 1800, 2033
Bst4CI	ACNGT	6 cut(s)	148, 826, 901, 950, 1553, 1885
Bst6I	CTCTTC	2 cut(s)	228, 1380
BstAPI	GCANNNNNTGC	1 cut(s)	767
BstAUI	TGTACA	1 cut(s)	168
BstC8I	GCNNGC	4 cut(s)	864, 942, 1408, 1619
BstDEI	CTNAG	7 cut(s)	215, 720, 848, 1632, 1959, 1993, 2048
BstDSI	CCRYGG	1 cut(s)	1221
BstF5I	GGATG	6 cut(s)	138, 228, 852, 1451, 1487, 1621
BstMAI	GTCTC	3 cut(s)	898, 1628, 1638
BstMWI	GCNNNNNNNGC	7 cut(s)	327, 557, 767, 1304, 1637, 1784, 1809
BstNI	CCWGG	7 cut(s)	511, 690, 1148, 1280, 1674, 1800, 2033
BstNSI	RCATGY	2 cut(s)	862, 944
BstSCI	CCNGG	8 cut(s)	509, 688, 1146, 1278, 1490, 1672, 1798, 2031
BstSFI	CTRYAG	1 cut(s)	1549
BstSLI	GKGCMC	1 cut(s)	980
BstV1I	GCAGC	4 cut(s)	181, 773, 1177, 1799
BstV2I	GAAGAC	1 cut(s)	366
BstX2I	RGATCY	3 cut(s)	175, 613, 1360
BstXI	CCANNNNNNTGG	3 cut(s)	1442, 1609, 1872
BstYI	RGATCY	3 cut(s)	175, 613, 1360
BsuRI	GGCC	4 cut(s)	627, 1677, 1812, 2014
BtgI	CCRYGG	1 cut(s)	1221
BtsCI	GGATG	6 cut(s)	138, 228, 852, 1451, 1487, 1621
BtsIMutI	CAGTG	4 cut(s)	822, 1477, 1558, 1858
Cac8I	GCNNGC	4 cut(s)	864, 942, 1408, 1619
CaiI	CAGNNNCTG	2 cut(s)	194, 1805
Cfr10I	RCCGGY	2 cut(s)	627, 1406
Cfr13I	GGNCC	6 cut(s)	70, 208, 514, 625, 1105, 1676
CsiI	ACCWGGT	1 cut(s)	1146
Csp6I	GTAC	5 cut(s)	98, 169, 333, 527, 817
CviQI	GTAC	5 cut(s)	98, 169, 333, 527, 817
DdeI	CTNAG	7 cut(s)	215, 720, 848, 1632, 1959, 1993, 2048
Eam1104I	CTCTTC	2 cut(s)	228, 1380
EarI	CTCTTC	2 cut(s)	228, 1380
EciI	GGCGGA	1 cut(s)	1682
Ecl136II	GAGCTC	1 cut(s)	398
Eco130I	CCWWGG	3 cut(s)	1221, 1504, 1813
Eco24I	GRGCYC	1 cut(s)	400
Eco32I	GATATC	1 cut(s)	706
Eco47I	GGWCC	4 cut(s)	70, 208, 514, 1105
Eco53kI	GAGCTC	1 cut(s)	398
Eco57I	CTGAAG	2 cut(s)	378, 1827
Eco88I	CYCGRG	2 cut(s)	198, 561
EcoICRI	GAGCTC	1 cut(s)	398
EcoO109I	RGGNCCY	3 cut(s)	70, 208, 514
EcoRI	GAATTC	1 cut(s)	1124
EcoRII	CCWGG	7 cut(s)	509, 688, 1146, 1278, 1672, 1798, 2031
EcoRV	GATATC	1 cut(s)	706
EcoT14I	CCWWGG	3 cut(s)	1221, 1504, 1813
EcoT22I	ATGCAT	2 cut(s)	942, 2021
EcoT38I	GRGCYC	1 cut(s)	400
ErhI	CCWWGG	3 cut(s)	1221, 1504, 1813
FaqI	GGGAC	5 cut(s)	221, 527, 1091, 1151, 1507
FauI	CCCGC	2 cut(s)	1186, 1633
FauNDI	CATATG	2 cut(s)	736, 1597
FblI	GTMKAC	3 cut(s)	465, 1527, 1988
Fnu4HI	GCNGC	5 cut(s)	192, 195, 762, 1191, 1788
FokI	GGATG	6 cut(s)	145, 235, 839, 1458, 1474, 1608
FriOI	GRGCYC	1 cut(s)	400
Fsp4HI	GCNGC	5 cut(s)	192, 195, 762, 1191, 1788
FspBI	CTAG	5 cut(s)	351, 405, 1082, 1259, 1400
GluI	GCNGC	5 cut(s)	192, 195, 762, 1191, 1788
GsuI	CTGGAG	1 cut(s)	901
HaeIII	GGCC	4 cut(s)	627, 1677, 1812, 2014
HapII	CCGG	3 cut(s)	628, 1407, 1491
HincII	GTYRAC	4 cut(s)	370, 832, 1528, 1989
HindII	GTYRAC	4 cut(s)	370, 832, 1528, 1989
HindIII	AAGCTT	1 cut(s)	1859
HpaI	GTTAAC	1 cut(s)	370
HpaII	CCGG	3 cut(s)	628, 1407, 1491
HphI	GGTGA	4 cut(s)	797, 1029, 1210, 1922
Hpy166II	GTNNAC	9 cut(s)	54, 335, 370, 466, 695, 832, 1218, 1528, 1989
Hpy188I	TCNGA	9 cut(s)	249, 390, 723, 874, 1009, 1313, 1807, 1880, 2043
Hpy188III	TCNNGA	5 cut(s)	230, 344, 554, 1153, 1562
Hpy8I	GTNNAC	9 cut(s)	54, 335, 370, 466, 695, 832, 1218, 1528, 1989
Hpy99I	CGWCG	1 cut(s)	1325
HpyAV	CCTTC	6 cut(s)	221, 385, 992, 1191, 1378, 1802
HpyCH4III	ACNGT	6 cut(s)	148, 826, 901, 950, 1553, 1885
HpyCH4IV	ACGT	1 cut(s)	1161
HpyF10VI	GCNNNNNNNGC	7 cut(s)	327, 557, 767, 1304, 1637, 1784, 1809
HpyF3I	CTNAG	7 cut(s)	215, 720, 848, 1632, 1959, 1993, 2048
HpySE526I	ACGT	1 cut(s)	1161
KflI	GGGWCCC	1 cut(s)	514
KpnI	GGTACC	1 cut(s)	101
KroI	GCCGGC	1 cut(s)	1406
KroNI	GCCGGC	1 cut(s)	1408
KspAI	GTTAAC	1 cut(s)	370
LmnI	GCTCC	4 cut(s)	395, 503, 595, 1972
Lsp1109I	GCAGC	4 cut(s)	181, 773, 1177, 1799
LweI	GCATC	6 cut(s)	430, 861, 927, 1177, 1630, 1765
MabI	ACCWGGT	1 cut(s)	1146
MaeI	CTAG	5 cut(s)	351, 405, 1082, 1259, 1400
MaeII	ACGT	1 cut(s)	1161
MaeIII	GTNAC	3 cut(s)	632, 641, 1978
MbiI	CCGCTC	1 cut(s)	321
MboII	GAAGA	7 cut(s)	245, 250, 371, 543, 662, 1345, 1367
MfeI	CAATTG	2 cut(s)	660, 1452
MflI	RGATCY	3 cut(s)	175, 613, 1360
MhlI	GDGCHC	2 cut(s)	400, 980
MlyI	GAGTC	4 cut(s)	220, 869, 1523, 1970
MmeI	TCCRAC	3 cut(s)	203, 902, 1608
MnlI	CCTC	8 cut(s)	286, 980, 1009, 1045, 1297, 1383, 1777, 2002
Mph1103I	ATGCAT	2 cut(s)	942, 2021
MroNI	GCCGGC	1 cut(s)	1406
MroXI	GAANNNNTTC	1 cut(s)	538
MseI	TTAA	7 cut(s)	369, 378, 810, 990, 1131, 1143, 1727
MslI	CAYNNNNRTG	7 cut(s)	422, 459, 662, 857, 1100, 1607, 1626
MspA1I	CMGCKG	2 cut(s)	191, 1997
MspI	CCGG	3 cut(s)	628, 1407, 1491
MspR9I	CCNGG	8 cut(s)	511, 690, 1148, 1280, 1492, 1674, 1800, 2033
MunI	CAATTG	2 cut(s)	660, 1452
Mva1269I	GAATGC	1 cut(s)	417
MvaI	CCWGG	7 cut(s)	511, 690, 1148, 1280, 1674, 1800, 2033
MwoI	GCNNNNNNNGC	7 cut(s)	327, 557, 767, 1304, 1637, 1784, 1809
NaeI	GCCGGC	1 cut(s)	1408
NciI	CCSGG	1 cut(s)	1492
NcoI	CCATGG	1 cut(s)	1221
NdeI	CATATG	2 cut(s)	736, 1597
NgoMIV	GCCGGC	1 cut(s)	1406
NlaIV	GGNNCC	9 cut(s)	72, 99, 209, 515, 516, 626, 979, 1107, 1671
NsiI	ATGCAT	2 cut(s)	942, 2021
NspI	RCATGY	2 cut(s)	862, 944
OliI	CACNNNNGTG	1 cut(s)	662
PaeI	GCATGC	1 cut(s)	944
PaeR7I	CTCGAG	1 cut(s)	198
PctI	GAATGC	1 cut(s)	417
PdiI	GCCGGC	1 cut(s)	1408
PdmI	GAANNNNTTC	1 cut(s)	538
PfeI	GAWTC	8 cut(s)	574, 1073, 1201, 1467, 1558, 1848, 1913, 2044
PflFI	GACNNNGTC	1 cut(s)	1097
PflMI	CCANNNNNTGG	1 cut(s)	740
PfoI	TCCNGGA	1 cut(s)	1490
PkrI	GCNGC	5 cut(s)	193, 196, 763, 1192, 1789
PleI	GAGTC	4 cut(s)	220, 869, 1523, 1969
PpsI	GAGTC	4 cut(s)	220, 869, 1523, 1969
PpuMI	RGGWCCY	3 cut(s)	70, 208, 514
PshBI	ATTAAT	3 cut(s)	378, 810, 1131
Psp124BI	GAGCTC	1 cut(s)	400
Psp1406I	AACGTT	1 cut(s)	1161
Psp5II	RGGWCCY	3 cut(s)	70, 208, 514
Psp6I	CCWGG	7 cut(s)	509, 688, 1146, 1278, 1672, 1798, 2031
PspGI	CCWGG	7 cut(s)	509, 688, 1146, 1278, 1672, 1798, 2031
PspN4I	GGNNCC	9 cut(s)	72, 99, 209, 515, 516, 626, 979, 1107, 1671
PspPI	GGNCC	6 cut(s)	70, 208, 514, 625, 1105, 1676
PspPPI	RGGWCCY	3 cut(s)	70, 208, 514
PspXI	VCTCGAGB	1 cut(s)	198
PstNI	CAGNNNCTG	2 cut(s)	194, 1805
PsuI	RGATCY	3 cut(s)	175, 613, 1360
PsyI	GACNNNGTC	1 cut(s)	1097
PvuII	CAGCTG	1 cut(s)	1997
RsaI	GTAC	5 cut(s)	99, 170, 334, 528, 818
RsaNI	GTAC	5 cut(s)	98, 169, 333, 527, 817
RseI	CAYNNNNRTG	7 cut(s)	422, 459, 662, 857, 1100, 1607, 1626
SacI	GAGCTC	1 cut(s)	400
SalI	GTCGAC	2 cut(s)	1526, 1987
SaqAI	TTAA	7 cut(s)	369, 378, 810, 990, 1131, 1143, 1727
SatI	GCNGC	5 cut(s)	192, 195, 762, 1191, 1788
Sau96I	GGNCC	6 cut(s)	70, 208, 514, 625, 1105, 1676
SchI	GAGTC	4 cut(s)	220, 869, 1523, 1970
ScrFI	CCNGG	8 cut(s)	511, 690, 1148, 1280, 1492, 1674, 1800, 2033
SduI	GDGCHC	2 cut(s)	400, 980
SexAI	ACCWGGT	1 cut(s)	1146
SfaNI	GCATC	6 cut(s)	430, 861, 927, 1177, 1630, 1765
SfcI	CTRYAG	1 cut(s)	1549
Sfr274I	CTCGAG	1 cut(s)	198
SinI	GGWCC	4 cut(s)	70, 208, 514, 1105
SlaI	CTCGAG	1 cut(s)	198
SmiMI	CAYNNNNRTG	7 cut(s)	422, 459, 662, 857, 1100, 1607, 1626
SmlI	CTYRAG	3 cut(s)	198, 971, 1531
SmoI	CTYRAG	3 cut(s)	198, 971, 1531
SphI	GCATGC	1 cut(s)	944
SsiI	CCGC	8 cut(s)	191, 321, 485, 602, 1040, 1193, 1640, 1667
SspI	AATATT	1 cut(s)	480
SspMI	CTAG	5 cut(s)	351, 405, 1082, 1259, 1400
SstI	GAGCTC	1 cut(s)	400
StyD4I	CCNGG	8 cut(s)	509, 688, 1146, 1278, 1490, 1672, 1798, 2031
StyI	CCWWGG	3 cut(s)	1221, 1504, 1813
TaaI	ACNGT	6 cut(s)	148, 826, 901, 950, 1553, 1885
TaiI	ACGT	1 cut(s)	1164
TaqI	TCGA	5 cut(s)	199, 1527, 1769, 1887, 1988
TatI	WGTACW	3 cut(s)	168, 332, 816
TauI	GCSGC	1 cut(s)	194
TfiI	GAWTC	8 cut(s)	574, 1073, 1201, 1467, 1558, 1848, 1913, 2044
Tru1I	TTAA	7 cut(s)	369, 378, 810, 990, 1131, 1143, 1727
Tru9I	TTAA	7 cut(s)	369, 378, 810, 990, 1131, 1143, 1727
TscAI	CASTG	4 cut(s)	829, 1477, 1558, 1858
TseI	GCWGC	4 cut(s)	194, 761, 1190, 1787
TspDTI	ATGAA	6 cut(s)	292, 305, 437, 1117, 1584, 1916
TspGWI	ACGGA	3 cut(s)	692, 977, 1309
TspRI	CASTG	4 cut(s)	829, 1477, 1558, 1858
Tth111I	GACNNNGTC	1 cut(s)	1097
Van91I	CCANNNNNTGG	1 cut(s)	740
VpaK11BI	GGWCC	4 cut(s)	70, 208, 514, 1105
VspI	ATTAAT	3 cut(s)	378, 810, 1131
XapI	RAATTY	3 cut(s)	715, 1124, 1395
XceI	RCATGY	2 cut(s)	862, 944
XhoI	CTCGAG	1 cut(s)	198
XmiI	GTMKAC	3 cut(s)	465, 1527, 1988
XmnI	GAANNNNTTC	1 cut(s)	538
XspI	CTAG	5 cut(s)	351, 405, 1082, 1259, 1400
Zsp2I	ATGCAT	2 cut(s)	942, 2021

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.