Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0000924

Physical Location & Seq

Genomic Coordinates Reverse (-)

476456 .. 480919

Gene Structure 4464 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1004200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1863 bp

ATGGGGCTTCTTCTCTCATGTTTCTTCATAGCTGTAGCAGAAGCAGAACATATTAGATATAAAGACCCCAAACAGCCGCTGAATACTCGAATCAAGGATCTATTGAGCCGGATGACGTTGGAGGAAAAGATTGGCCAAATGGTGCAGATAGACCGCAGTGTTGCTTCGTCTGAGGTGATGAAGAAATACTTCATTGGGAGTATATTAAGCGGGGGTGGGAGCGTTCCAGCACAAAGGGCTTCTCCAGAAGCATGGATTAACATGGTGAATGATTTTCAAAAAGGTTCTTTATCAACCCGGCTTGGAATTCCAATGATTTATGGTATCGATGCAGTTCATGGCCACAACAACGTCTACAAAGCAACAATCTTTCCGCACAATATTGGCCTTGGAGCTACCAGGGACCCCGTACTTGTTAAGAGGATCGGAGCTGCAACTGCACTTGAAGCTAGAGCTACAGGCATTCCATATGTCTTCGCACCTTGTATAGCGGTTTGCAGAGATCCAAGATGGGGTCGATGTTATGAAAGCTACAGTGAAGATCACAAAATTGTTCAAGCAATGACTGAGATCATACCTGGATTACAAGGGGAGTTACCAGCCAACTCTAGAAAGGGCATTCCCTTTGTGGGCGGAAATAAAAAGGTTGCAGCGTGTGCAAAGCATTACGTCGGTGATGGAGGAACAACTAGGGGCATCAACGAGAATAACACTGTGATAAACAGACACGGCTTGCTCAGTATCCACATGCCAGGCTACTATGACTCAATTATCAAAGGGGTTGCAACTATTATGGTATCTTACTCGAGCTGGAATGGAGTAAAGATGCATGCTAATCATGATCTTGTTACTGGCTTCCTTAAGAACACTCTTCATTTCAGGGGTTTTGTCATCTCAGATTGGGAGGGTATTGACAGGATCACGTCTCCACCTCATGCCAACTACTCATATTCAATTCAAGCAGGCGTCAATGCCGGAATTGACATGGTCATGATTCCATACAACTATACGGAATTTATTGACGGTCTAACCTCCCTGGTGAAAAATGGAATCATTCCCATGAGCAGAATTGATGATGCTGTAAAAAGAATTTTGCGGGTTAAGTTCGTCATGGGCTTATTTGAGGAACCGTTGGCTGATCGCAGCCTGGTCGACCAACTTGGGAGTAAGGAACACAGAGAATTAGCTAGGGAAGCTGTGAGGAGATCTCTAGTGCTTCTAAAGAACGGCAAATCTGCTGATGAGCTGTTGCTTCCCCTTCCGAAGAAGGCATCAAAAATACTTGTAGCTGGTAGTCATGCAGATAACCTTGGTTATCAGTGTGGTGGGTGGACCATTGAGTGGCAGGGCTTAAGTGGCAACAATCTCACCGAAGGTACCACAATTCTTAGTGCCATTAAGAACACAGTGGATCCTAAAACCAAAGTAGTGTACAAGGAGAATCCTGATGCTGACTTTGTGAAGTCGAATGATTTCTCATATGCCATCGTTGTGGTAGGGGAACACCCGTACGCAGAGACGTTTGGTGACAGCCTAAACTTGACAATACCGGATCCTGGCCCGAGTGCCATCACAAATGTTTGCGGTTCTGTGAAATGTGTTGTTATCGTCATCTCTGGCCGTCCTGTTGTGATCCAACCATATGTTCCCTTGATGGATGCTCTTGTTGCAGCTTGGCTTCCAGGAACGGAAGGGCAGGGCGTAGCTGATGTTTTATTTGGTGACTATGGCTTCACGGGCAAGCTTTCTCGAACCTGGTTCAAAACCGTTGATCAGCTGCCGATGAATGTAGGAGACGCACATTATGACCCTCTCTTCCCCTTTGGATATGGCCTCACTACAACAACGGCCAATTCCAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0000323 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005886 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0008422 GO:0009056 GO:0009057 GO:0009251 GO:0015926 GO:0016020 GO:0016052 GO:0016787 GO:0016798 GO:0042597 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0071704 GO:0071944 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00460 ko00500 ko00940 ko01100 ko01110 map00460 map00500 map00940 map01100 map01110

Pfam Domains

Protein Families

Glyco_hydro_3 Glyco_hydro_3_C

Protein Analysis

621

Amino Acids

67.61

Weight (kDa)

7.99

Isoelectric Point (pI)

30.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_3	PF00933	39 - 367	1.9e-69	Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C	PF01915	404 - 613	3.4e-37	Glycosyl hydrolase family 3 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000924)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04885 AT5G04885 AT5G04885
fragaria_vesca	FvH4_1g28352 FvH4_1g28352 FvH4_1g28352 FvH4_4g26180 FvH4_4g26180 FvH4_7g05220 FvH4_7g05220
malus_domestica	MD02G1259600.v1.1 MD07G1060900.v1.1 MD11G1177800.v1.1 MD11G1177900.v1.1 MD14G1004200.v1.1
prunus_persica	Prupe.2G065700_v2.0.a1 Prupe.2G065700_v2.0.a1 Prupe.2G065700_v2.0.a1 Prupe.2G065700_v2.0.a1 Prupe.2G065700_v2.0.a1 Prupe.6G150000_v2.0.a1 Prupe.6G150200_v2.0.a1 Prupe.6G150400_v2.0.a1 Prupe.6G150500_v2.0.a1 Prupe.7G007200_v2.0.a1
pyrus_communis	pycom14g00250
rosa_chinensis	RchiOBHm_Chr1g0331601 RchiOBHm_Chr3g0496681 RchiOBHm_Chr3g0496721 RchiOBHm_Chr4g0433771
rosa_laevigata	RLG00000006732 RLG00000022510 RLG00000022511 RLG00000029727 RLG00000034619
rosa_multiflora	Rmu_sc0005044.1_g000017 Rmu_sc0006176.1_g000006
rosa_roxburghii	Rroxscaffold_4G00319030 Rroxscaffold_5G00375200 Rroxscaffold_6G00388790 Rroxscaffold_6G00388830 Rroxscaffold_6G00388850
rosa_rugosa	Rorug01G0095000 Rorug01G0095100 Rorug01G0095200 Rorug03G0185800 Rorug03G0186000 Rorug03G0285500 Rorug03G0285700 Rorug04G0271300 Rorug05G0248600 Rorug06G0034700
rosa_samantha	Rh1AG118800 Rh1CG113800 Rh3AG330600 Rh3BG366600 Rh3CG363300 Rh3CG363500 Rh4AG326000 Rh4CG349100 Rh5AG325000
rosa_wichuraiana	Rw1G009560 Rw3G028940 Rw3G028950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1376
AccB1I	GGYRCC	1 cut(s)	1376
AccB7I	CCANNNNNTGG	1 cut(s)	1341
AccI	GTMKAC	2 cut(s)	354, 1152
AciI	CCGC	8 cut(s)	77, 154, 210, 374, 491, 633, 1096, 1584
AclWI	GGATC	9 cut(s)	105, 431, 497, 926, 1406, 1419, 1547, 1560, 1627
AcoI	YGGCCR	4 cut(s)	133, 340, 1618, 1848
AcsI	RAATTY	3 cut(s)	306, 1013, 1089
AcyI	GRCGYC	1 cut(s)	966
AfaI	GTAC	4 cut(s)	411, 1378, 1433, 1511
AfiI	CCNNNNNNNGG	4 cut(s)	512, 629, 1341, 1556
AflII	CTTAAG	2 cut(s)	860, 1351
AgsI	TTSAA	6 cut(s)	278, 446, 557, 954, 959, 1762
AjiI	CACGTC	1 cut(s)	924
AjnI	CCWGG	8 cut(s)	398, 577, 751, 1035, 1146, 1555, 1681, 1754
AjuI	GAANNNNNNNTTGG	2 cut(s)	1629, 1661
Alw26I	GTCTC	3 cut(s)	930, 1511, 1788
AlwI	GGATC	9 cut(s)	105, 431, 497, 926, 1406, 1419, 1547, 1560, 1627
AlwNI	CAGNNNCTG	1 cut(s)	79
Ama87I	CYCGRG	2 cut(s)	805, 1561
AoxI	GGCC	7 cut(s)	133, 340, 385, 1558, 1618, 1831, 1848
ApeKI	GCWGC	5 cut(s)	431, 650, 1143, 1670, 1777
ApoI	RAATTY	3 cut(s)	306, 1013, 1089
Asp700I	GAANNNNTTC	2 cut(s)	188, 1472
Asp718I	GGTACC	1 cut(s)	1376
AspS9I	GGNCC	3 cut(s)	403, 1332, 1559
AsuC2I	CCSGG	1 cut(s)	298
AsuHPI	GGTGA	7 cut(s)	187, 277, 686, 1051, 1360, 1538, 1733
AvaI	CYCGRG	2 cut(s)	805, 1561
AvaII	GGWCC	2 cut(s)	403, 1332
BalI	TGGCCA	2 cut(s)	135, 342
BamHI	GGATCC	2 cut(s)	1411, 1552
BanI	GGYRCC	1 cut(s)	1376
BbsI	GAAGAC	1 cut(s)	466
BbvI	GCAGC	5 cut(s)	418, 662, 1155, 1682, 1764
BccI	CCATC	5 cut(s)	504, 671, 1493, 1577, 1648
BceAI	ACGGC	3 cut(s)	745, 1243, 1605
BcgI	CGANNNNNNTGC	2 cut(s)	1132, 1166
BciT130I	CCWGG	8 cut(s)	400, 579, 753, 1037, 1148, 1557, 1683, 1756
BciVI	GTATCC	1 cut(s)	752
BclI	TGATCA	1 cut(s)	1771
BcnI	CCSGG	1 cut(s)	298
BcoDI	GTCTC	3 cut(s)	930, 1511, 1788
BfaI	CTAG	6 cut(s)	450, 609, 690, 1188, 1211, 1861
BfmI	CTRYAG	3 cut(s)	33, 456, 532
BfrI	CTTAAG	2 cut(s)	860, 1351
BfuI	GTATCC	1 cut(s)	752
BglII	AGATCT	1 cut(s)	1205
BisI	GCNGC	6 cut(s)	77, 432, 651, 1144, 1671, 1778
BlsI	GCNGC	6 cut(s)	78, 433, 652, 1145, 1672, 1779
Bme1390I	CCNGG	9 cut(s)	298, 400, 579, 753, 1037, 1148, 1557, 1683, 1756
Bme18I	GGWCC	2 cut(s)	403, 1332
BmeT110I	CYCGRG	2 cut(s)	805, 1561
BmgBI	CACGTC	1 cut(s)	924
BmgT120I	GGNCC	3 cut(s)	403, 1332, 1559
BmiI	GGNNCC	6 cut(s)	404, 405, 1128, 1378, 1413, 1554
BmrFI	CCNGG	9 cut(s)	298, 400, 579, 753, 1037, 1148, 1557, 1683, 1756
BmsI	GCATC	7 cut(s)	319, 705, 816, 1066, 1280, 1438, 1648
BpiI	GAAGAC	1 cut(s)	466
BplI	GAGNNNNNCTC	2 cut(s)	1192, 1224
BpmI	CTGGAG	1 cut(s)	228
BpuMI	CCSGG	1 cut(s)	298
Bsa29I	ATCGAT	1 cut(s)	327
BsaHI	GRCGYC	1 cut(s)	966
BsaJI	CCNNGG	4 cut(s)	388, 399, 1035, 1309
BsaWI	WCCGGW	1 cut(s)	1549
Bsc4I	CCNNNNNNNGG	4 cut(s)	512, 629, 1341, 1556
Bse1I	ACTGG	1 cut(s)	856
Bse3DI	GCAATG	1 cut(s)	567
BseBI	CCWGG	8 cut(s)	400, 579, 753, 1037, 1148, 1557, 1683, 1756
BseCI	ATCGAT	1 cut(s)	327
BseDI	CCNNGG	4 cut(s)	388, 399, 1035, 1309
BseGI	GGATG	2 cut(s)	117, 1663
BseLI	CCNNNNNNNGG	4 cut(s)	512, 629, 1341, 1556
BseMI	GCAATG	1 cut(s)	567
BseMII	CTCAG	4 cut(s)	162, 558, 751, 909
BseNI	ACTGG	1 cut(s)	856
BseRI	GAGGAG	1 cut(s)	1216
BseXI	GCAGC	5 cut(s)	418, 662, 1155, 1682, 1764
BsgI	GTGCAG	2 cut(s)	164, 423
BshFI	GGCC	7 cut(s)	135, 342, 387, 1560, 1620, 1833, 1850
BshNI	GGYRCC	1 cut(s)	1376
BshVI	ATCGAT	1 cut(s)	327
BsiHKCI	CYCGRG	2 cut(s)	805, 1561
BsiSI	CCGG	4 cut(s)	109, 298, 975, 1550
BsiWI	CGTACG	1 cut(s)	1509
BslFI	GGGAC	1 cut(s)	416
BslI	CCNNNNNNNGG	4 cut(s)	512, 629, 1341, 1556
BsmAI	GTCTC	3 cut(s)	930, 1511, 1788
BsmBI	CGTCTC	3 cut(s)	930, 1511, 1788
BsmFI	GGGAC	1 cut(s)	416
BsmI	GAATGC	2 cut(s)	462, 618
BsnI	GGCC	7 cut(s)	135, 342, 387, 1560, 1620, 1833, 1850
BsoBI	CYCGRG	2 cut(s)	805, 1561
Bsp1407I	TGTACA	1 cut(s)	1431
BspACI	CCGC	8 cut(s)	77, 154, 210, 374, 491, 633, 1096, 1584
BspANI	GGCC	7 cut(s)	135, 342, 387, 1560, 1620, 1833, 1850
BspCNI	CTCAG	4 cut(s)	163, 559, 750, 908
BspDI	ATCGAT	1 cut(s)	327
BspHI	TCATGA	2 cut(s)	838, 990
BspLI	GGNNCC	6 cut(s)	404, 405, 1128, 1378, 1413, 1554
BspPI	GGATC	9 cut(s)	105, 431, 497, 926, 1406, 1419, 1547, 1560, 1627
BspT107I	GGYRCC	1 cut(s)	1376
BspTI	CTTAAG	2 cut(s)	860, 1351
BsrDI	GCAATG	1 cut(s)	567
BsrGI	TGTACA	1 cut(s)	1431
BsrI	ACTGG	1 cut(s)	856
BssECI	CCNNGG	4 cut(s)	388, 399, 1035, 1309
BssNI	GRCGYC	1 cut(s)	966
BssT1I	CCWWGG	2 cut(s)	388, 1309
Bst2UI	CCWGG	8 cut(s)	400, 579, 753, 1037, 1148, 1557, 1683, 1756
Bst4CI	ACNGT	6 cut(s)	536, 715, 1025, 1131, 1408, 1768
Bst6I	CTCTTC	2 cut(s)	876, 1820
BstACI	GRCGYC	1 cut(s)	966
BstAFI	CTTAAG	2 cut(s)	860, 1351
BstAPI	GCANNNNNTGC	1 cut(s)	656
BstAUI	TGTACA	1 cut(s)	1431
BstC8I	GCNNGC	4 cut(s)	734, 831, 964, 1742
BstDEI	CTNAG	5 cut(s)	171, 567, 737, 895, 1388
BstF5I	GGATG	2 cut(s)	117, 1663
BstMAI	GTCTC	3 cut(s)	930, 1511, 1788
BstMWI	GCNNNNNNNGC	8 cut(s)	236, 437, 446, 656, 1193, 1356, 1667, 1737
BstNI	CCWGG	8 cut(s)	400, 579, 753, 1037, 1148, 1557, 1683, 1756
BstNSI	RCATGY	2 cut(s)	751, 833
BstSCI	CCNGG	9 cut(s)	296, 398, 577, 751, 1035, 1146, 1555, 1681, 1754
BstSFI	CTRYAG	3 cut(s)	33, 456, 532
BstV1I	GCAGC	5 cut(s)	418, 662, 1155, 1682, 1764
BstV2I	GAAGAC	1 cut(s)	466
BstX2I	RGATCY	5 cut(s)	97, 502, 1205, 1411, 1552
BstXI	CCANNNNNNTGG	2 cut(s)	252, 1492
BstYI	RGATCY	5 cut(s)	97, 502, 1205, 1411, 1552
Bsu15I	ATCGAT	1 cut(s)	327
BsuI	GTATCC	1 cut(s)	752
BsuRI	GGCC	7 cut(s)	135, 342, 387, 1560, 1620, 1833, 1850
BsuTUI	ATCGAT	1 cut(s)	327
BtrI	CACGTC	1 cut(s)	924
BtsCI	GGATG	2 cut(s)	117, 1663
BtsI	GCAGTG	1 cut(s)	163
BtsIMutI	CAGTG	5 cut(s)	163, 541, 711, 1325, 1413
Cac8I	GCNNGC	4 cut(s)	734, 831, 964, 1742
CaiI	CAGNNNCTG	1 cut(s)	79
CciI	TCATGA	2 cut(s)	838, 990
Cfr13I	GGNCC	3 cut(s)	403, 1332, 1559
ClaI	ATCGAT	1 cut(s)	327
CseI	GACGC	2 cut(s)	955, 1805
CsiI	ACCWGGT	1 cut(s)	1754
Csp6I	GTAC	4 cut(s)	410, 1377, 1432, 1510
CviQI	GTAC	4 cut(s)	410, 1377, 1432, 1510
DdeI	CTNAG	5 cut(s)	171, 567, 737, 895, 1388
EaeI	YGGCCR	4 cut(s)	133, 340, 1618, 1848
Eam1104I	CTCTTC	2 cut(s)	876, 1820
EarI	CTCTTC	2 cut(s)	876, 1820
EciI	GGCGGA	1 cut(s)	648
Eco130I	CCWWGG	2 cut(s)	388, 1309
Eco47I	GGWCC	2 cut(s)	403, 1332
Eco88I	CYCGRG	2 cut(s)	805, 1561
EcoO109I	RGGNCCY	1 cut(s)	403
EcoRI	GAATTC	1 cut(s)	306
EcoRII	CCWGG	8 cut(s)	398, 577, 751, 1035, 1146, 1555, 1681, 1754
EcoT14I	CCWWGG	2 cut(s)	388, 1309
EcoT22I	ATGCAT	1 cut(s)	831
ErhI	CCWWGG	2 cut(s)	388, 1309
Esp3I	CGTCTC	3 cut(s)	930, 1511, 1788
FalI	AAGNNNNNCTT	2 cut(s)	173, 205
FaqI	GGGAC	1 cut(s)	416
FauI	CCCGC	2 cut(s)	203, 1089
FauNDI	CATATG	3 cut(s)	469, 1480, 1642
FbaI	TGATCA	1 cut(s)	1771
FblI	GTMKAC	2 cut(s)	354, 1152
Fnu4HI	GCNGC	6 cut(s)	77, 432, 651, 1144, 1671, 1778
FokI	GGATG	2 cut(s)	124, 1670
Fsp4HI	GCNGC	6 cut(s)	77, 432, 651, 1144, 1671, 1778
FspBI	CTAG	6 cut(s)	450, 609, 690, 1188, 1211, 1861
GluI	GCNGC	6 cut(s)	77, 432, 651, 1144, 1671, 1778
GsuI	CTGGAG	1 cut(s)	228
HaeIII	GGCC	7 cut(s)	135, 342, 387, 1560, 1620, 1833, 1850
HapII	CCGG	4 cut(s)	109, 298, 975, 1550
HgaI	GACGC	2 cut(s)	955, 1805
Hin1I	GRCGYC	1 cut(s)	966
HincII	GTYRAC	1 cut(s)	1153
HindII	GTYRAC	1 cut(s)	1153
HindIII	AAGCTT	1 cut(s)	1742
HinfI	GANTC	5 cut(s)	90, 764, 994, 1050, 1441
HpaII	CCGG	4 cut(s)	109, 298, 975, 1550
HphI	GGTGA	7 cut(s)	187, 277, 686, 1051, 1360, 1538, 1733
Hpy166II	GTNNAC	4 cut(s)	355, 1153, 1332, 1432
Hpy188I	TCNGA	4 cut(s)	172, 428, 898, 1263
Hpy188III	TCNNGA	6 cut(s)	245, 609, 839, 991, 1445, 1749
Hpy8I	GTNNAC	4 cut(s)	355, 1153, 1332, 1432
Hpy99I	CGWCG	1 cut(s)	674
HpyAV	CCTTC	4 cut(s)	1261, 1268, 1367, 1685
HpyCH4III	ACNGT	6 cut(s)	536, 715, 1025, 1131, 1408, 1768
HpyCH4IV	ACGT	5 cut(s)	116, 351, 669, 923, 1520
HpyF10VI	GCNNNNNNNGC	8 cut(s)	236, 437, 446, 656, 1193, 1356, 1667, 1737
HpyF3I	CTNAG	5 cut(s)	171, 567, 737, 895, 1388
HpySE526I	ACGT	5 cut(s)	116, 351, 669, 923, 1520
Hsp92I	GRCGYC	1 cut(s)	966
KflI	GGGWCCC	1 cut(s)	403
KpnI	GGTACC	1 cut(s)	1380
Ksp22I	TGATCA	1 cut(s)	1771
LmnI	GCTCC	3 cut(s)	219, 392, 428
Lsp1109I	GCAGC	5 cut(s)	418, 662, 1155, 1682, 1764
LweI	GCATC	7 cut(s)	319, 705, 816, 1066, 1280, 1438, 1648
MabI	ACCWGGT	1 cut(s)	1754
MaeI	CTAG	6 cut(s)	450, 609, 690, 1188, 1211, 1861
MaeII	ACGT	5 cut(s)	116, 351, 669, 923, 1520
MaeIII	GTNAC	4 cut(s)	594, 847, 1526, 1721
MboII	GAAGA	7 cut(s)	16, 193, 466, 551, 863, 1276, 1807
MflI	RGATCY	5 cut(s)	97, 502, 1205, 1411, 1552
MlsI	TGGCCA	2 cut(s)	135, 342
MluNI	TGGCCA	2 cut(s)	135, 342
MlyI	GAGTC	1 cut(s)	758
MmeI	TCCRAC	2 cut(s)	99, 1660
Mox20I	TGGCCA	2 cut(s)	135, 342
Mph1103I	ATGCAT	1 cut(s)	831
MroXI	GAANNNNTTC	2 cut(s)	188, 1472
MscI	TGGCCA	2 cut(s)	135, 342
MseI	TTAA	7 cut(s)	206, 258, 417, 861, 1101, 1352, 1398
MslI	CAYNNNNRTG	3 cut(s)	989, 1058, 1490
Msp20I	TGGCCA	2 cut(s)	135, 342
MspA1I	CMGCKG	2 cut(s)	79, 1777
MspCI	CTTAAG	2 cut(s)	860, 1351
MspI	CCGG	4 cut(s)	109, 298, 975, 1550
MspR9I	CCNGG	9 cut(s)	298, 400, 579, 753, 1037, 1148, 1557, 1683, 1756
Mva1269I	GAATGC	2 cut(s)	462, 618
MvaI	CCWGG	8 cut(s)	400, 579, 753, 1037, 1148, 1557, 1683, 1756
MwoI	GCNNNNNNNGC	8 cut(s)	236, 437, 446, 656, 1193, 1356, 1667, 1737
NciI	CCSGG	1 cut(s)	298
NdeI	CATATG	3 cut(s)	469, 1480, 1642
NlaIV	GGNNCC	6 cut(s)	404, 405, 1128, 1378, 1413, 1554
NmuCI	GTSAC	2 cut(s)	1526, 1721
NsiI	ATGCAT	1 cut(s)	831
NspI	RCATGY	2 cut(s)	751, 833
PaeI	GCATGC	1 cut(s)	833
PaeR7I	CTCGAG	1 cut(s)	805
PagI	TCATGA	2 cut(s)	838, 990
PctI	GAATGC	2 cut(s)	462, 618
PdmI	GAANNNNTTC	2 cut(s)	188, 1472
PfeI	GAWTC	4 cut(s)	90, 994, 1050, 1441
Pfl23II	CGTACG	1 cut(s)	1509
PflFI	GACNNNGTC	1 cut(s)	986
PflMI	CCANNNNNTGG	1 cut(s)	1341
PfoI	TCCNGGA	1 cut(s)	1681
PkrI	GCNGC	6 cut(s)	78, 433, 652, 1145, 1672, 1779
PleI	GAGTC	1 cut(s)	758
PpsI	GAGTC	1 cut(s)	758
PpuMI	RGGWCCY	1 cut(s)	403
Psp5II	RGGWCCY	1 cut(s)	403
Psp6I	CCWGG	8 cut(s)	398, 577, 751, 1035, 1146, 1555, 1681, 1754
PspGI	CCWGG	8 cut(s)	398, 577, 751, 1035, 1146, 1555, 1681, 1754
PspLI	CGTACG	1 cut(s)	1509
PspN4I	GGNNCC	6 cut(s)	404, 405, 1128, 1378, 1413, 1554
PspPI	GGNCC	3 cut(s)	403, 1332, 1559
PspPPI	RGGWCCY	1 cut(s)	403
PspXI	VCTCGAGB	1 cut(s)	805
PstNI	CAGNNNCTG	1 cut(s)	79
PsuI	RGATCY	5 cut(s)	97, 502, 1205, 1411, 1552
PsyI	GACNNNGTC	1 cut(s)	986
PvuII	CAGCTG	1 cut(s)	1777
RsaI	GTAC	4 cut(s)	411, 1378, 1433, 1511
RsaNI	GTAC	4 cut(s)	410, 1377, 1432, 1510
RseI	CAYNNNNRTG	3 cut(s)	989, 1058, 1490
SalI	GTCGAC	1 cut(s)	1151
SaqAI	TTAA	7 cut(s)	206, 258, 417, 861, 1101, 1352, 1398
SatI	GCNGC	6 cut(s)	77, 432, 651, 1144, 1671, 1778
Sau96I	GGNCC	3 cut(s)	403, 1332, 1559
SchI	GAGTC	1 cut(s)	758
ScrFI	CCNGG	9 cut(s)	298, 400, 579, 753, 1037, 1148, 1557, 1683, 1756
SexAI	ACCWGGT	1 cut(s)	1754
SfaNI	GCATC	7 cut(s)	319, 705, 816, 1066, 1280, 1438, 1648
SfcI	CTRYAG	3 cut(s)	33, 456, 532
Sfr274I	CTCGAG	1 cut(s)	805
SinI	GGWCC	2 cut(s)	403, 1332
SlaI	CTCGAG	1 cut(s)	805
SmiMI	CAYNNNNRTG	3 cut(s)	989, 1058, 1490
SmlI	CTYRAG	3 cut(s)	805, 860, 1351
SmoI	CTYRAG	3 cut(s)	805, 860, 1351
SphI	GCATGC	1 cut(s)	833
SsiI	CCGC	8 cut(s)	77, 154, 210, 374, 491, 633, 1096, 1584
SspI	AATATT	1 cut(s)	382
SspMI	CTAG	6 cut(s)	450, 609, 690, 1188, 1211, 1861
StyD4I	CCNGG	9 cut(s)	296, 398, 577, 751, 1035, 1146, 1555, 1681, 1754
StyI	CCWWGG	2 cut(s)	388, 1309
TaaI	ACNGT	6 cut(s)	536, 715, 1025, 1131, 1408, 1768
TaiI	ACGT	5 cut(s)	119, 354, 672, 926, 1523
TaqI	TCGA	7 cut(s)	88, 327, 517, 806, 1152, 1466, 1750
TatI	WGTACW	1 cut(s)	1431
TauI	GCSGC	1 cut(s)	79
TfiI	GAWTC	4 cut(s)	90, 994, 1050, 1441
Tru1I	TTAA	7 cut(s)	206, 258, 417, 861, 1101, 1352, 1398
Tru9I	TTAA	7 cut(s)	206, 258, 417, 861, 1101, 1352, 1398
TscAI	CASTG	5 cut(s)	163, 541, 718, 1325, 1413
TseFI	GTSAC	2 cut(s)	1526, 1721
TseI	GCWGC	5 cut(s)	431, 650, 1143, 1670, 1777
Tsp45I	GTSAC	2 cut(s)	1526, 1721
TspDTI	ATGAA	7 cut(s)	16, 181, 194, 326, 540, 863, 1799
TspGWI	ACGGA	2 cut(s)	1025, 1703
TspRI	CASTG	5 cut(s)	163, 541, 718, 1325, 1413
Tth111I	GACNNNGTC	1 cut(s)	986
Van91I	CCANNNNNTGG	1 cut(s)	1341
Vha464I	CTTAAG	2 cut(s)	860, 1351
VpaK11BI	GGWCC	2 cut(s)	403, 1332
XapI	RAATTY	3 cut(s)	306, 1013, 1089
XbaI	TCTAGA	1 cut(s)	608
XceI	RCATGY	2 cut(s)	751, 833
XhoI	CTCGAG	1 cut(s)	805
XmiI	GTMKAC	2 cut(s)	354, 1152
XmnI	GAANNNNTTC	2 cut(s)	188, 1472
XspI	CTAG	6 cut(s)	450, 609, 690, 1188, 1211, 1861
Zsp2I	ATGCAT	1 cut(s)	831

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.