Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0000924

Physical Location & Seq

Genomic Coordinates Reverse (-)

46502407 .. 46514174

Gene Structure 11768 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00319030.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1644 bp

ATGATTTATGGCATTGACGCTGTTCATGGACACAATAACGTCTATAACGCTACAATATTTCCGCATAATGTTGGGCTTGGAGCGACCAGGGATCCTGAACTGGCGAAAAGAATTGGTGCTGCAACTGCTCTTGAAGTCCGAGCTACAGGCATTCCTTATGCTTTTGCTCCATGCATTGCGGTATGTAGAGATCCACGATGGGGTCGCTGTTATGAAAGCTACAGCGAGGATCACAAAATTGTGCAAGCAATGACAGAAATTATTCCTGGTTTACAGGGAGATATTCCTGCTAATTCTCGGAAAGGAGTTCCATATGTTGGCGGAAATAAAAAGGTTGCTGCTTGTGCAAAGCATTTTGTGGGTGATGGTGGGACAAGCAAGGGCATTAATGAGAACAACACCGTTATTGACCGACATGATTTGCTTAGCATCCACATGCCTGCTTATTCTGATTCGATTATCAAGGGTGTCTCCACAGTGATGATTTCGTACTCCAGTTGGAATGGGGAGAAGATGCATGCAAACCGTGACCTAGTCACTGGCTTCCTTAAAGAGACCCTTAAGTTTAAGGGTTTTGTTATATCAGACTGGGAGGGAATTGACAGGATTACCTCGCCACCACATTCAAACTACACATACTCTGTCCAAGCTTCAATTCTTGCTGGAATAGACATGGTAATGGTTCCTGTTAACTATAAAGAGTTCATCAATGATCTCACCTACCTGATCAAGACCAATGTTATCCCAATGGATCGTATTGATGATGCTGTCGGAAGGATTTTGCTTGTCAAGTTTACCATGGGTCTTTTTGAGAACCCTTTGGGTGATCTCAGCCTAGTCAATGAGCTTGGAAGCCAGGCTCATAGAGATTTGGCAAGGGAGGCTGTCAGAAAGTCACTTGTGCTTCTGAAGAATGGGAAAAATGGAACCAATCCGGTCCTACCTCTTCCTAAGAAGGTGCCCAAAATCTTGGTTGCTGGCAGTCATGCTGATAATCTGGGTTACCAGTGTGGTGGATGGACAATTGCTTGGCAAGGATTCAGTGGCAACAACTTTACAAGGGGAACTACTGTTCTTGGTGCTATCAAATCAACAGTTGATTCAAGCACAGAAGTTATCTATCATGAGAATCCTGATGGTGAATTTGTTAAGTCCAACAACTTTGAATATGCCATTGTTGTTGTTGGCGAGCACCCTTATGCCGAGACTTATGGAGACAGCATGAACTTAACTATGGCGGATCCTGGCCCAAGTGTCATCACCAGTGTGTGTGAAGCTGTCAAGTGCATTGTTATTATAATTTCTGGCAGACCTGTTGTGATCGAACCATATATTTCCTCAATTGATGCCCTGGTGGCAGCATGGTTGCCAGGAACTGAAGGCCAAGGCATTACTGATGTCCTTTATGGCGAGTATGGATTCAGCGGAAAGCTTTCGAGAACATGGTTCAGAAGTGTAGATCAACTTCCAATGAATGTTGGGGATCCACACTATGATCCACTTTTCCCTTTTGGGTTTGGACTTGAAACTGAGTCGGTTAAGGAGCTCGTAGCAAGGTCAACTTCAGCTGGTGTATTTGGAAGGCCATGCACACTTCTCATTGTCGTTGCTCTAATTCTCAGCTTCAGACAAGAGTTTCTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0000323 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005886 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0008422 GO:0009056 GO:0009057 GO:0009251 GO:0015926 GO:0016020 GO:0016052 GO:0016787 GO:0016798 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0071704 GO:0071944 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00460 ko00500 ko00940 ko01100 ko01110 map00460 map00500 map00940 map01100 map01110

Pfam Domains

Protein Families

Glyco_hydro_3 Glyco_hydro_3_C

Protein Analysis

547

Amino Acids

59.58

Weight (kDa)

5.98

Isoelectric Point (pI)

26.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_3	PF00933	2 - 261	4.4e-57	Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C	PF01915	300 - 509	1.1e-36	Glycosyl hydrolase family 3 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000924)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04885 AT5G04885 AT5G04885
fragaria_vesca	FvH4_1g28352 FvH4_1g28352 FvH4_1g28352 FvH4_4g26180 FvH4_4g26180 FvH4_7g05220 FvH4_7g05220
malus_domestica	MD02G1259600.v1.1 MD07G1060900.v1.1 MD11G1177800.v1.1 MD11G1177900.v1.1 MD14G1004200.v1.1
prunus_persica	Prupe.2G065700_v2.0.a1 Prupe.2G065700_v2.0.a1 Prupe.2G065700_v2.0.a1 Prupe.2G065700_v2.0.a1 Prupe.2G065700_v2.0.a1 Prupe.6G150000_v2.0.a1 Prupe.6G150200_v2.0.a1 Prupe.6G150400_v2.0.a1 Prupe.6G150500_v2.0.a1 Prupe.7G007200_v2.0.a1
pyrus_communis	pycom14g00250
rosa_chinensis	RchiOBHm_Chr1g0331601 RchiOBHm_Chr3g0496681 RchiOBHm_Chr3g0496721 RchiOBHm_Chr4g0433771
rosa_laevigata	RLG00000006732 RLG00000022510 RLG00000022511 RLG00000029727 RLG00000034619
rosa_multiflora	Rmu_sc0005044.1_g000017 Rmu_sc0006176.1_g000006
rosa_roxburghii	Rroxscaffold_4G00319030 Rroxscaffold_5G00375200 Rroxscaffold_6G00388790 Rroxscaffold_6G00388830 Rroxscaffold_6G00388850
rosa_rugosa	Rorug01G0095000 Rorug01G0095100 Rorug01G0095200 Rorug03G0185800 Rorug03G0186000 Rorug03G0285500 Rorug03G0285700 Rorug04G0271300 Rorug05G0248600 Rorug06G0034700
rosa_samantha	Rh1AG118800 Rh1CG113800 Rh3AG330600 Rh3BG366600 Rh3CG363300 Rh3CG363500 Rh4AG326000 Rh4CG349100 Rh5AG325000
rosa_wichuraiana	Rw1G009560 Rw3G028940 Rw3G028950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1298
AccB1I	GGYRCC	1 cut(s)	958
AccB7I	CCANNNNNTGG	1 cut(s)	317
AciI	CCGC	5 cut(s)	62, 179, 321, 1238, 1425
AcsI	RAATTY	1 cut(s)	1142
AcuI	CTGAAG	4 cut(s)	929, 1398, 1548, 1609
AfaI	GTAC	1 cut(s)	491
AfiI	CCNNNNNNNGG	2 cut(s)	200, 317
AflII	CTTAAG	1 cut(s)	560
AgsI	TTSAA	6 cut(s)	134, 627, 654, 1104, 1166, 1526
AjnI	CCWGG	6 cut(s)	86, 265, 855, 1243, 1350, 1369
AjuI	GAANNNNNNNTTGG	2 cut(s)	639, 671
AleI	CACNNNNGTG	1 cut(s)	1265
AloI	GAACNNNNNNTCC	2 cut(s)	1056, 1088
AluBI	AGCT	9 cut(s)	143, 219, 650, 847, 1277, 1432, 1546, 1568, 1623
AluI	AGCT	9 cut(s)	143, 219, 650, 847, 1277, 1432, 1546, 1568, 1623
Alw21I	GWGCWC	2 cut(s)	1194, 1548
Alw26I	GTCTC	4 cut(s)	475, 548, 1199, 1209
AlwNI	CAGNNNCTG	1 cut(s)	1376
AoxI	GGCC	3 cut(s)	1246, 1381, 1583
ApeKI	GCWGC	3 cut(s)	119, 338, 1358
ApoI	RAATTY	1 cut(s)	1142
AseI	ATTAAT	1 cut(s)	387
Asp700I	GAANNNNTTC	2 cut(s)	261, 1432
AspS9I	GGNCC	2 cut(s)	937, 1247
AsuHPI	GGTGA	5 cut(s)	374, 709, 836, 1151, 1252
AvaII	GGWCC	1 cut(s)	937
BaeGI	GKGCMC	1 cut(s)	963
BamHI	GGATCC	3 cut(s)	91, 1240, 1483
BanI	GGYRCC	1 cut(s)	958
BanII	GRGCYC	1 cut(s)	1548
BarI	GAAGNNNNNNTAC	2 cut(s)	1449, 1481
Bbv12I	GWGCWC	2 cut(s)	1194, 1548
BbvI	GCAGC	3 cut(s)	106, 325, 1370
BccI	CCATC	4 cut(s)	192, 359, 1011, 1130
BciT130I	CCWGG	6 cut(s)	88, 267, 857, 1245, 1352, 1371
BclI	TGATCA	1 cut(s)	726
BcoDI	GTCTC	4 cut(s)	475, 548, 1199, 1209
BfaI	CTAG	2 cut(s)	533, 836
BfmI	CTRYAG	3 cut(s)	144, 220, 1640
BfrI	CTTAAG	1 cut(s)	560
BglI	GCCNNNNNGGC	1 cut(s)	1355
BisI	GCNGC	3 cut(s)	120, 339, 1359
BlpI	GCTNAGC	1 cut(s)	425
BlsI	GCNGC	3 cut(s)	121, 340, 1360
Bme1390I	CCNGG	6 cut(s)	88, 267, 857, 1245, 1352, 1371
Bme18I	GGWCC	1 cut(s)	937
BmgT120I	GGNCC	2 cut(s)	937, 1247
BmiI	GGNNCC	6 cut(s)	93, 684, 928, 960, 1242, 1485
BmrFI	CCNGG	6 cut(s)	88, 267, 857, 1245, 1352, 1371
BmrI	ACTGGG	1 cut(s)	598
BmsI	GCATC	4 cut(s)	438, 504, 754, 1336
BmuI	ACTGGG	1 cut(s)	598
BpmI	CTGGAG	1 cut(s)	478
Bpu1102I	GCTNAGC	1 cut(s)	425
BsaI	GGTCTC	1 cut(s)	548
BsaJI	CCNNGG	4 cut(s)	87, 798, 1350, 1384
BsaWI	WCCGGW	1 cut(s)	934
Bsc4I	CCNNNNNNNGG	2 cut(s)	200, 317
Bse1I	ACTGG	6 cut(s)	105, 495, 544, 593, 1006, 1263
Bse3DI	GCAATG	2 cut(s)	174, 255
BseBI	CCWGG	6 cut(s)	88, 267, 857, 1245, 1352, 1371
BseDI	CCNNGG	4 cut(s)	87, 798, 1350, 1384
BseGI	GGATG	2 cut(s)	429, 1022
BseLI	CCNNNNNNNGG	2 cut(s)	200, 317
BseMI	GCAATG	2 cut(s)	174, 255
BseMII	CTCAG	3 cut(s)	844, 1521, 1633
BseNI	ACTGG	6 cut(s)	105, 495, 544, 593, 1006, 1263
BseSI	GKGCMC	1 cut(s)	963
BseXI	GCAGC	3 cut(s)	106, 325, 1370
BshFI	GGCC	3 cut(s)	1248, 1383, 1585
BshNI	GGYRCC	1 cut(s)	958
BsiHKAI	GWGCWC	2 cut(s)	1194, 1548
BsiSI	CCGG	1 cut(s)	935
BslFI	GGGAC	1 cut(s)	385
BslI	CCNNNNNNNGG	2 cut(s)	200, 317
BsmAI	GTCTC	4 cut(s)	475, 548, 1199, 1209
BsmFI	GGGAC	1 cut(s)	385
BsmI	GAATGC	1 cut(s)	150
BsnI	GGCC	3 cut(s)	1248, 1383, 1585
Bso31I	GGTCTC	1 cut(s)	548
Bsp1286I	GDGCHC	3 cut(s)	963, 1194, 1548
Bsp1720I	GCTNAGC	1 cut(s)	425
Bsp19I	CCATGG	1 cut(s)	798
BspACI	CCGC	5 cut(s)	62, 179, 321, 1238, 1425
BspANI	GGCC	3 cut(s)	1248, 1383, 1585
BspCNI	CTCAG	3 cut(s)	843, 1522, 1632
BspHI	TCATGA	1 cut(s)	1123
BspLI	GGNNCC	6 cut(s)	93, 684, 928, 960, 1242, 1485
BspT107I	GGYRCC	1 cut(s)	958
BspTI	CTTAAG	1 cut(s)	560
BspTNI	GGTCTC	1 cut(s)	548
BsrDI	GCAATG	2 cut(s)	174, 255
BsrI	ACTGG	6 cut(s)	105, 495, 544, 593, 1006, 1263
BssECI	CCNNGG	4 cut(s)	87, 798, 1350, 1384
BssT1I	CCWWGG	2 cut(s)	798, 1384
Bst2UI	CCWGG	6 cut(s)	88, 267, 857, 1245, 1352, 1371
Bst4CI	ACNGT	5 cut(s)	403, 478, 527, 1072, 1096
Bst6I	CTCTTC	1 cut(s)	951
BstAFI	CTTAAG	1 cut(s)	560
BstC8I	GCNNGC	5 cut(s)	246, 441, 519, 979, 1190
BstDEI	CTNAG	5 cut(s)	425, 830, 951, 1530, 1619
BstDSI	CCRYGG	1 cut(s)	798
BstEII	GGTNACC	1 cut(s)	1001
BstF5I	GGATG	2 cut(s)	429, 1022
BstMAI	GTCTC	4 cut(s)	475, 548, 1199, 1209
BstMWI	GCNNNNNNNGC	4 cut(s)	125, 344, 881, 1355
BstNI	CCWGG	6 cut(s)	88, 267, 857, 1245, 1352, 1371
BstNSI	RCATGY	2 cut(s)	439, 521
BstPI	GGTNACC	1 cut(s)	1001
BstSCI	CCNGG	6 cut(s)	86, 265, 855, 1243, 1350, 1369
BstSFI	CTRYAG	3 cut(s)	144, 220, 1640
BstSLI	GKGCMC	1 cut(s)	963
BstV1I	GCAGC	3 cut(s)	106, 325, 1370
BstX2I	RGATCY	4 cut(s)	91, 190, 1240, 1483
BstXI	CCANNNNNNTGG	2 cut(s)	970, 1013
BstYI	RGATCY	4 cut(s)	91, 190, 1240, 1483
BsuRI	GGCC	3 cut(s)	1248, 1383, 1585
BtgI	CCRYGG	1 cut(s)	798
BtsCI	GGATG	2 cut(s)	429, 1022
BtsIMutI	CAGTG	5 cut(s)	483, 537, 1013, 1048, 1270
Cac8I	GCNNGC	5 cut(s)	246, 441, 519, 979, 1190
CaiI	CAGNNNCTG	1 cut(s)	1376
CciI	TCATGA	1 cut(s)	1123
Cfr13I	GGNCC	2 cut(s)	937, 1247
CseI	GACGC	1 cut(s)	26
Csp6I	GTAC	1 cut(s)	490
CviQI	GTAC	1 cut(s)	490
DdeI	CTNAG	5 cut(s)	425, 830, 951, 1530, 1619
Eam1104I	CTCTTC	1 cut(s)	951
EarI	CTCTTC	1 cut(s)	951
EciI	GGCGGA	2 cut(s)	336, 1253
Ecl136II	GAGCTC	1 cut(s)	1546
Eco130I	CCWWGG	2 cut(s)	798, 1384
Eco24I	GRGCYC	1 cut(s)	1548
Eco31I	GGTCTC	1 cut(s)	548
Eco47I	GGWCC	1 cut(s)	937
Eco53kI	GAGCTC	1 cut(s)	1546
Eco57I	CTGAAG	4 cut(s)	929, 1398, 1548, 1609
Eco91I	GGTNACC	1 cut(s)	1001
EcoICRI	GAGCTC	1 cut(s)	1546
EcoO65I	GGTNACC	1 cut(s)	1001
EcoRII	CCWGG	6 cut(s)	86, 265, 855, 1243, 1350, 1369
EcoT14I	CCWWGG	2 cut(s)	798, 1384
EcoT22I	ATGCAT	2 cut(s)	176, 519
EcoT38I	GRGCYC	1 cut(s)	1548
ErhI	CCWWGG	2 cut(s)	798, 1384
FalI	AAGNNNNNCTT	4 cut(s)	543, 575, 1546, 1578
FaqI	GGGAC	1 cut(s)	385
FauNDI	CATATG	1 cut(s)	313
FbaI	TGATCA	1 cut(s)	726
Fnu4HI	GCNGC	3 cut(s)	120, 339, 1359
FokI	GGATG	2 cut(s)	416, 1029
FriOI	GRGCYC	1 cut(s)	1548
Fsp4HI	GCNGC	3 cut(s)	120, 339, 1359
FspBI	CTAG	2 cut(s)	533, 836
GluI	GCNGC	3 cut(s)	120, 339, 1359
GsuI	CTGGAG	1 cut(s)	478
HaeIII	GGCC	3 cut(s)	1248, 1383, 1585
HapII	CCGG	1 cut(s)	935
HgaI	GACGC	1 cut(s)	26
HincII	GTYRAC	2 cut(s)	691, 1560
HindII	GTYRAC	2 cut(s)	691, 1560
HindIII	AAGCTT	2 cut(s)	648, 1430
HinfI	GANTC	6 cut(s)	452, 1038, 1100, 1129, 1419, 1532
HpaI	GTTAAC	1 cut(s)	691
HpaII	CCGG	1 cut(s)	935
HphI	GGTGA	5 cut(s)	374, 709, 836, 1151, 1252
Hpy166II	GTNNAC	4 cut(s)	272, 691, 795, 1560
Hpy188I	TCNGA	9 cut(s)	140, 300, 451, 586, 773, 890, 909, 1451, 1628
Hpy188III	TCNNGA	6 cut(s)	95, 131, 730, 1124, 1133, 1437
Hpy8I	GTNNAC	4 cut(s)	272, 691, 795, 1560
HpyAV	CCTTC	4 cut(s)	768, 949, 1373, 1575
HpyCH4III	ACNGT	5 cut(s)	403, 478, 527, 1072, 1096
HpyCH4IV	ACGT	1 cut(s)	39
HpyCH4V	TGCA	8 cut(s)	122, 174, 244, 347, 517, 521, 1287, 1590
HpyF10VI	GCNNNNNNNGC	4 cut(s)	125, 344, 881, 1355
HpyF3I	CTNAG	5 cut(s)	425, 830, 951, 1530, 1619
HpySE526I	ACGT	1 cut(s)	39
Ksp22I	TGATCA	1 cut(s)	726
KspAI	GTTAAC	1 cut(s)	691
LmnI	GCTCC	3 cut(s)	80, 172, 1543
Lsp1109I	GCAGC	3 cut(s)	106, 325, 1370
LweI	GCATC	4 cut(s)	438, 504, 754, 1336
MaeI	CTAG	2 cut(s)	533, 836
MaeII	ACGT	1 cut(s)	39
MaeIII	GTNAC	4 cut(s)	527, 535, 894, 1001
MboII	GAAGA	3 cut(s)	523, 922, 938
MfeI	CAATTG	2 cut(s)	1023, 1341
MflI	RGATCY	4 cut(s)	91, 190, 1240, 1483
MhlI	GDGCHC	3 cut(s)	963, 1194, 1548
MlyI	GAGTC	1 cut(s)	1541
MmeI	TCCRAC	3 cut(s)	479, 751, 1179
MnlI	CCTC	6 cut(s)	220, 586, 622, 874, 954, 1348
Mph1103I	ATGCAT	2 cut(s)	176, 519
MroXI	GAANNNNTTC	2 cut(s)	261, 1432
MseI	TTAA	8 cut(s)	387, 549, 561, 567, 690, 1149, 1229, 1539
MslI	CAYNNNNRTG	5 cut(s)	434, 479, 677, 1197, 1265
MspA1I	CMGCKG	2 cut(s)	1425, 1568
MspCI	CTTAAG	1 cut(s)	560
MspI	CCGG	1 cut(s)	935
MspR9I	CCNGG	6 cut(s)	88, 267, 857, 1245, 1352, 1371
MunI	CAATTG	2 cut(s)	1023, 1341
Mva1269I	GAATGC	1 cut(s)	150
MvaI	CCWGG	6 cut(s)	88, 267, 857, 1245, 1352, 1371
MwoI	GCNNNNNNNGC	4 cut(s)	125, 344, 881, 1355
NcoI	CCATGG	1 cut(s)	798
NdeI	CATATG	1 cut(s)	313
NlaIV	GGNNCC	6 cut(s)	93, 684, 928, 960, 1242, 1485
NmeAIII	GCCGAG	1 cut(s)	1228
NmuCI	GTSAC	3 cut(s)	527, 535, 894
NsiI	ATGCAT	2 cut(s)	176, 519
NspI	RCATGY	2 cut(s)	439, 521
OliI	CACNNNNGTG	1 cut(s)	1265
PaeI	GCATGC	1 cut(s)	521
PagI	TCATGA	1 cut(s)	1123
PctI	GAATGC	1 cut(s)	150
PdmI	GAANNNNTTC	2 cut(s)	261, 1432
PfeI	GAWTC	5 cut(s)	452, 1038, 1100, 1129, 1419
PflFI	GACNNNGTC	1 cut(s)	533
PflMI	CCANNNNNTGG	1 cut(s)	317
PkrI	GCNGC	3 cut(s)	121, 340, 1360
PleI	GAGTC	1 cut(s)	1540
PpsI	GAGTC	1 cut(s)	1540
PshBI	ATTAAT	1 cut(s)	387
PsiI	TTATAA	1 cut(s)	1298
Psp124BI	GAGCTC	1 cut(s)	1548
Psp6I	CCWGG	6 cut(s)	86, 265, 855, 1243, 1350, 1369
PspEI	GGTNACC	1 cut(s)	1001
PspGI	CCWGG	6 cut(s)	86, 265, 855, 1243, 1350, 1369
PspN4I	GGNNCC	6 cut(s)	93, 684, 928, 960, 1242, 1485
PspPI	GGNCC	2 cut(s)	937, 1247
PstNI	CAGNNNCTG	1 cut(s)	1376
PsuI	RGATCY	4 cut(s)	91, 190, 1240, 1483
PsyI	GACNNNGTC	1 cut(s)	533
PvuII	CAGCTG	1 cut(s)	1568
RsaI	GTAC	1 cut(s)	491
RsaNI	GTAC	1 cut(s)	490
RseI	CAYNNNNRTG	5 cut(s)	434, 479, 677, 1197, 1265
SacI	GAGCTC	1 cut(s)	1548
SaqAI	TTAA	8 cut(s)	387, 549, 561, 567, 690, 1149, 1229, 1539
SatI	GCNGC	3 cut(s)	120, 339, 1359
Sau96I	GGNCC	2 cut(s)	937, 1247
SchI	GAGTC	1 cut(s)	1541
ScrFI	CCNGG	6 cut(s)	88, 267, 857, 1245, 1352, 1371
SduI	GDGCHC	3 cut(s)	963, 1194, 1548
SfaNI	GCATC	4 cut(s)	438, 504, 754, 1336
SfcI	CTRYAG	3 cut(s)	144, 220, 1640
SinI	GGWCC	1 cut(s)	937
SmiMI	CAYNNNNRTG	5 cut(s)	434, 479, 677, 1197, 1265
SmlI	CTYRAG	1 cut(s)	560
SmoI	CTYRAG	1 cut(s)	560
SphI	GCATGC	1 cut(s)	521
SsiI	CCGC	5 cut(s)	62, 179, 321, 1238, 1425
SspI	AATATT	1 cut(s)	57
SspMI	CTAG	2 cut(s)	533, 836
SstI	GAGCTC	1 cut(s)	1548
StyD4I	CCNGG	6 cut(s)	86, 265, 855, 1243, 1350, 1369
StyI	CCWWGG	2 cut(s)	798, 1384
TaaI	ACNGT	5 cut(s)	403, 478, 527, 1072, 1096
TaiI	ACGT	1 cut(s)	42
TaqI	TCGA	3 cut(s)	455, 1323, 1436
TaqII	GACCGA	1 cut(s)	426
TfiI	GAWTC	5 cut(s)	452, 1038, 1100, 1129, 1419
Tru1I	TTAA	8 cut(s)	387, 549, 561, 567, 690, 1149, 1229, 1539
Tru9I	TTAA	8 cut(s)	387, 549, 561, 567, 690, 1149, 1229, 1539
TscAI	CASTG	5 cut(s)	483, 544, 1013, 1048, 1270
TseFI	GTSAC	3 cut(s)	527, 535, 894
TseI	GCWGC	3 cut(s)	119, 338, 1358
Tsp45I	GTSAC	3 cut(s)	527, 535, 894
TspDTI	ATGAA	5 cut(s)	14, 228, 694, 1238, 1487
TspRI	CASTG	5 cut(s)	483, 544, 1013, 1048, 1270
Tth111I	GACNNNGTC	1 cut(s)	533
Van91I	CCANNNNNTGG	1 cut(s)	317
Vha464I	CTTAAG	1 cut(s)	560
VpaK11BI	GGWCC	1 cut(s)	937
VspI	ATTAAT	1 cut(s)	387
XapI	RAATTY	1 cut(s)	1142
XceI	RCATGY	2 cut(s)	439, 521
XmnI	GAANNNNTTC	2 cut(s)	261, 1432
XspI	CTAG	2 cut(s)	533, 836
Zsp2I	ATGCAT	2 cut(s)	176, 519

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.