Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr3

Orthogroup ID

OG0000924

Physical Location & Seq

Genomic Coordinates Forward (+)

44009321 .. 44014734

Gene Structure 5414 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw3G028950.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1956 bp

ATGATGAGCTTGCAAACAATTTGGAGAAAATATGAAATAGCTAGGATGTCTGCCTCCTTGACGAGGGCACTTCTTCTGTTATGCTTGTGTATAGCAGCGGCAACGATGAGAACAGCAGAAGCAGCAGGTGGAGGAGGATACATGAAATATAAGGACCCCAAACAGCCCTTGAATACTAGAATCAATGACCTACTGAGCCGGATGACTTTGGAGGAAAAGATTGGCCAGATGACGCAGATAGAGCGCATTGTTGCTTCAGCTGAGGTCATGAACAAGTACTACATTGGGAGTGTGTTTAGCGGCGCTGTGAGTGTGCCATCAGAAAAAGATTCTGCAGAAACTTGGGTTACAATGGTGAATGATTTTCAAAAGGGTTCTTTATCAACTCGCCTTGGTATTCCAATGATTTATGGTATTGATGCTGTTCATGGCCACAATAATGTCTACAAGGCAACAATCTTTCCTCACAATGTTGGCCTTGGAGCTACGAGGGACCCTGAACTTGTTAAAAGGATTGGAGCTGCAACTGCACTTGAAGTTAGAGCCACAGGCATTTCATACGTCTTTGCACCTTGCATAGCGGTTTGTAGAGATCCAAGATGGGGTCGTTGTTACGAAAGCTACAGTGAAGATCATAAGGTTGTTCAAGCAATGACTCAGATCATACCTGGATTACAAGGAGACATACCACCTAACTCTAGAAAGGGTGTTCCCTTTGTAGCAGCTGGAAATAAAAAGGTTGCAGCCTGTGCAAAGCATTATGTGGGCGATGGTGGAACAACCAAAGGCATCGATGAGGATAACACTGTAATAAACAGGCATGGGTTACTCAGCATTCACATGCCACCCTACTATAACTCAATTATCGAAGGTGTTGCAACCATTATGGTATCTTACTCCAGCTGGAATGGAGTCAAGATGCATGCAAACCGTGATCTGGTCACTGGCTTTCTTAAGAATACTCTCCGTTTCAGGGGTTTTGTCATATCAGATTTCATGGGTATTGACAGAATTACCTCACCAGCTTATGCCAACTACTCATACTCGATTACAGCAGGGATCAATGCTGGCATTGACATGATCATGGTTCCATACAACTATACAGAATTCATTGATGGTCTAACTTTCCAGGCAAAAAATAAAATCATTCCCATGAGCAGAATTGATGATGCAGTGAGGAGAATTTTGCGGGTTAAGTTTATAATGGGGCTATTTGAGAATCCGTTGGCTGATCTCAGCCTCGTCAACCAGCTTGGCAGTCAGGAACACAGAGACTTGGCTCGGGAAGCTGTGAGGCGATCGCTAGTTCTTCTAAAGAATGGTGAATCTGCAGACAAGCCATTGCTGCCCCTTCCAAAGAAGACATCAAAAATACTTGTAGCTGGAAGTCATGCAGACAATCTTGGTTATCAGTGTGGTGGTTGGACCATTGAGTGGCAGGGATTGAGTGGCAACGATCTCACACATGGTACCACAATCCTAACCGGTATAAAGAACACAGTTGATCCAGTAACTGAAGTGGTGTACAAGGAGAATCCTGATGCTGAGTATGTTAAGTCAAATGATTTCTCCTATGCCATCGTTGTCGTTGGAGAATCACCATATGCAGAGACATTTGGTGACAGTTTAACTTTGACTCTCCCTGATGCGGGCCTTAAAACCATTACAAATGTTTGTGGTGCTGTGAAGTGTGTTGTCATCATCATCTCCGGTCGTCCTGTTGTGATCCAACCATACGTTCCCCTGCTAGATGCTCTGGTTGCGGCGTGGCTTCCAGGAAGTGAGGGCCAAGGAGTTGCTGATGTTTTGTTTGGTGACTATGGTTTCACCGGCAAGCTTTCTCGCACTTGGTTTAAAACCGTTGATCAGCTACCTATGAATGTTGGAGATCCACATTATGACCCTCTCTTCCCCTTTGGATTTGGCCTGACAACAACACCCACCAAAGCCAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0000323 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005886 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0008422 GO:0009056 GO:0009057 GO:0009251 GO:0015926 GO:0016020 GO:0016052 GO:0016787 GO:0016798 GO:0042597 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0071704 GO:0071944 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00460 ko00500 ko00940 ko01100 ko01110 map00460 map00500 map00940 map01100 map01110

Pfam Domains

Protein Families

Glyco_hydro_3 Glyco_hydro_3_C

Protein Analysis

651

Amino Acids

71.13

Weight (kDa)

8.19

Isoelectric Point (pI)

32.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_3	PF00933	69 - 398	5.2e-67	Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C	PF01915	435 - 644	8.3e-39	Glycosyl hydrolase family 3 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000924)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04885 AT5G04885 AT5G04885
fragaria_vesca	FvH4_1g28352 FvH4_1g28352 FvH4_1g28352 FvH4_4g26180 FvH4_4g26180 FvH4_7g05220 FvH4_7g05220
malus_domestica	MD02G1259600.v1.1 MD07G1060900.v1.1 MD11G1177800.v1.1 MD11G1177900.v1.1 MD14G1004200.v1.1
prunus_persica	Prupe.2G065700_v2.0.a1 Prupe.2G065700_v2.0.a1 Prupe.2G065700_v2.0.a1 Prupe.2G065700_v2.0.a1 Prupe.2G065700_v2.0.a1 Prupe.6G150000_v2.0.a1 Prupe.6G150200_v2.0.a1 Prupe.6G150400_v2.0.a1 Prupe.6G150500_v2.0.a1 Prupe.7G007200_v2.0.a1
pyrus_communis	pycom14g00250
rosa_chinensis	RchiOBHm_Chr1g0331601 RchiOBHm_Chr3g0496681 RchiOBHm_Chr3g0496721 RchiOBHm_Chr4g0433771
rosa_laevigata	RLG00000006732 RLG00000022510 RLG00000022511 RLG00000029727 RLG00000034619
rosa_multiflora	Rmu_sc0005044.1_g000017 Rmu_sc0006176.1_g000006
rosa_roxburghii	Rroxscaffold_4G00319030 Rroxscaffold_5G00375200 Rroxscaffold_6G00388790 Rroxscaffold_6G00388830 Rroxscaffold_6G00388850
rosa_rugosa	Rorug01G0095000 Rorug01G0095100 Rorug01G0095200 Rorug03G0185800 Rorug03G0186000 Rorug03G0285500 Rorug03G0285700 Rorug04G0271300 Rorug05G0248600 Rorug06G0034700
rosa_samantha	Rh1AG118800 Rh1CG113800 Rh3AG330600 Rh3BG366600 Rh3CG363300 Rh3CG363500 Rh4AG326000 Rh4CG349100 Rh5AG325000
rosa_wichuraiana	Rw1G009560 Rw3G028940 Rw3G028950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1202
AarI	CACCTGC	1 cut(s)	116
Acc36I	ACCTGC	1 cut(s)	116
Acc65I	GGTACC	1 cut(s)	1469
AccB1I	GGYRCC	1 cut(s)	1469
AccB7I	CCANNNNNTGG	1 cut(s)	1434
AccI	GTMKAC	1 cut(s)	444
AciI	CCGC	6 cut(s)	98, 300, 581, 1189, 1649, 1763
AclWI	GGATC	5 cut(s)	587, 1067, 1499, 1720, 1883
AcoI	YGGCCR	2 cut(s)	223, 430
AcsI	RAATTY	2 cut(s)	1106, 1182
AcuI	CTGAAG	2 cut(s)	240, 1536
AfaI	GTAC	3 cut(s)	278, 1471, 1526
AfiI	CCNNNNNNNGG	7 cut(s)	63, 602, 937, 973, 1434, 1648, 1649
AflII	CTTAAG	1 cut(s)	953
AgeI	ACCGGT	1 cut(s)	1484
AgsI	TTSAA	4 cut(s)	172, 368, 536, 647
AjnI	CCWGG	3 cut(s)	667, 1128, 1774
AjuI	GAANNNNNNNTTGG	2 cut(s)	1722, 1754
Alw26I	GTCTC	3 cut(s)	675, 1266, 1604
AlwI	GGATC	5 cut(s)	587, 1067, 1499, 1720, 1883
AlwNI	CAGNNNCTG	1 cut(s)	1514
Ama87I	CYCGRG	1 cut(s)	1281
AoxI	GGCC	6 cut(s)	223, 430, 475, 1651, 1786, 1924
ApeKI	GCWGC	6 cut(s)	95, 122, 521, 722, 743, 1345
ApoI	RAATTY	2 cut(s)	1106, 1182
AsiGI	ACCGGT	1 cut(s)	1484
AsiSI	GCGATCGC	1 cut(s)	1301
Asp718I	GGTACC	1 cut(s)	1469
AspLEI	GCGC	2 cut(s)	246, 305
AspS9I	GGNCC	5 cut(s)	154, 493, 1425, 1651, 1786
AsuHPI	GGTGA	7 cut(s)	367, 1011, 1334, 1590, 1631, 1819, 1826
AvaI	CYCGRG	1 cut(s)	1281
AvaII	GGWCC	3 cut(s)	154, 493, 1425
BaeGI	GKGCMC	1 cut(s)	70
BalI	TGGCCA	2 cut(s)	225, 432
BanI	GGYRCC	1 cut(s)	1469
BbsI	GAAGAC	1 cut(s)	1367
BbvCI	CCTCAGC	1 cut(s)	261
BbvI	GCAGC	6 cut(s)	107, 134, 508, 734, 755, 1332
BccI	CCATC	5 cut(s)	325, 594, 764, 1109, 1586
BcgI	CGANNNNNNTGC	2 cut(s)	857, 891
BciT130I	CCWGG	3 cut(s)	669, 1130, 1776
BciVI	GTATCC	1 cut(s)	131
BclI	TGATCA	2 cut(s)	1080, 1864
BcoDI	GTCTC	3 cut(s)	675, 1266, 1604
BfaI	CTAG	6 cut(s)	42, 177, 699, 1304, 1748, 1954
BfmI	CTRYAG	3 cut(s)	333, 622, 1329
BfoI	RGCGCY	1 cut(s)	306
BfrI	CTTAAG	1 cut(s)	953
BfuAI	ACCTGC	1 cut(s)	116
BfuI	GTATCC	1 cut(s)	131
BisI	GCNGC	9 cut(s)	96, 99, 123, 301, 522, 723, 744, 1346, 1764
BlsI	GCNGC	9 cut(s)	97, 100, 124, 302, 523, 724, 745, 1347, 1765
BmcAI	AGTACT	1 cut(s)	278
Bme1390I	CCNGG	3 cut(s)	669, 1130, 1776
Bme18I	GGWCC	3 cut(s)	154, 493, 1425
BmeT110I	CYCGRG	1 cut(s)	1281
BmgT120I	GGNCC	5 cut(s)	154, 493, 1425, 1651, 1786
BmiI	GGNNCC	5 cut(s)	156, 494, 495, 1089, 1471
BmrFI	CCNGG	3 cut(s)	669, 1130, 1776
BmsI	GCATC	7 cut(s)	409, 798, 909, 1159, 1531, 1636, 1741
BpiI	GAAGAC	1 cut(s)	1367
BpmI	CTGGAG	1 cut(s)	883
Bpu10I	CCTNAGC	1 cut(s)	261
Bsa29I	ATCGAT	1 cut(s)	792
BsaJI	CCNNGG	3 cut(s)	391, 478, 1789
BsaWI	WCCGGW	2 cut(s)	1484, 1709
Bsc4I	CCNNNNNNNGG	7 cut(s)	63, 602, 937, 973, 1434, 1648, 1649
Bse118I	RCCGGY	2 cut(s)	1484, 1829
Bse1I	ACTGG	2 cut(s)	949, 1508
Bse3DI	GCAATG	2 cut(s)	657, 1340
BseBI	CCWGG	3 cut(s)	669, 1130, 1776
BseCI	ATCGAT	1 cut(s)	792
BseDI	CCNNGG	3 cut(s)	391, 478, 1789
BseGI	GGATG	2 cut(s)	51, 207
BseLI	CCNNNNNNNGG	7 cut(s)	63, 602, 937, 973, 1434, 1648, 1649
BseMI	GCAATG	2 cut(s)	657, 1340
BseMII	CTCAG	6 cut(s)	185, 252, 671, 844, 1249, 1536
BseNI	ACTGG	2 cut(s)	949, 1508
BseRI	GAGGAG	2 cut(s)	147, 1192
BseSI	GKGCMC	1 cut(s)	70
BseXI	GCAGC	6 cut(s)	107, 134, 508, 734, 755, 1332
BsgI	GTGCAG	1 cut(s)	513
Bsh1285I	CGRYCG	2 cut(s)	1301, 1714
BshFI	GGCC	6 cut(s)	225, 432, 477, 1653, 1788, 1926
BshNI	GGYRCC	1 cut(s)	1469
BshTI	ACCGGT	1 cut(s)	1484
BshVI	ATCGAT	1 cut(s)	792
BsiEI	CGRYCG	2 cut(s)	1301, 1714
BsiHKCI	CYCGRG	1 cut(s)	1281
BsiSI	CCGG	4 cut(s)	199, 1485, 1710, 1830
BslFI	GGGAC	1 cut(s)	506
BslI	CCNNNNNNNGG	7 cut(s)	63, 602, 937, 973, 1434, 1648, 1649
BsmAI	GTCTC	3 cut(s)	675, 1266, 1604
BsmFI	GGGAC	1 cut(s)	506
BsmI	GAATGC	1 cut(s)	834
BsnI	GGCC	6 cut(s)	225, 432, 477, 1653, 1788, 1926
BsoBI	CYCGRG	1 cut(s)	1281
Bsp1286I	GDGCHC	1 cut(s)	70
Bsp1407I	TGTACA	1 cut(s)	1524
BspACI	CCGC	6 cut(s)	98, 300, 581, 1189, 1649, 1763
BspANI	GGCC	6 cut(s)	225, 432, 477, 1653, 1788, 1926
BspCNI	CTCAG	6 cut(s)	186, 253, 670, 843, 1248, 1537
BspDI	ATCGAT	1 cut(s)	792
BspHI	TCATGA	1 cut(s)	267
BspLI	GGNNCC	5 cut(s)	156, 494, 495, 1089, 1471
BspMAI	CTGCAG	2 cut(s)	337, 1333
BspMI	ACCTGC	1 cut(s)	116
BspPI	GGATC	5 cut(s)	587, 1067, 1499, 1720, 1883
BspT107I	GGYRCC	1 cut(s)	1469
BspTI	CTTAAG	1 cut(s)	953
BsrDI	GCAATG	2 cut(s)	657, 1340
BsrFI	RCCGGY	2 cut(s)	1484, 1829
BsrGI	TGTACA	1 cut(s)	1524
BsrI	ACTGG	2 cut(s)	949, 1508
BssAI	RCCGGY	2 cut(s)	1484, 1829
BssECI	CCNNGG	3 cut(s)	391, 478, 1789
BssT1I	CCWWGG	3 cut(s)	391, 478, 1789
Bst2UI	CCWGG	3 cut(s)	669, 1130, 1776
Bst4CI	ACNGT	6 cut(s)	626, 808, 932, 1501, 1625, 1861
Bst6I	CTCTTC	1 cut(s)	1913
BstAFI	CTTAAG	1 cut(s)	953
BstAPI	GCANNNNNTGC	1 cut(s)	749
BstAUI	TGTACA	1 cut(s)	1524
BstC8I	GCNNGC	5 cut(s)	11, 924, 1069, 1651, 1835
BstDEI	CTNAG	6 cut(s)	194, 261, 657, 830, 1235, 1545
BstENI	CCTNNNNNAGG	1 cut(s)	61
BstF5I	GGATG	2 cut(s)	51, 207
BstH2I	RGCGCY	1 cut(s)	306
BstHHI	GCGC	2 cut(s)	246, 305
BstMAI	GTCTC	3 cut(s)	675, 1266, 1604
BstMCI	CGRYCG	2 cut(s)	1301, 1714
BstMWI	GCNNNNNNNGC	7 cut(s)	122, 241, 527, 749, 1286, 1345, 1760
BstNI	CCWGG	3 cut(s)	669, 1130, 1776
BstNSI	RCATGY	2 cut(s)	844, 926
BstSCI	CCNGG	3 cut(s)	667, 1128, 1774
BstSFI	CTRYAG	3 cut(s)	333, 622, 1329
BstSLI	GKGCMC	1 cut(s)	70
BstV1I	GCAGC	6 cut(s)	107, 134, 508, 734, 755, 1332
BstV2I	GAAGAC	1 cut(s)	1367
BstX2I	RGATCY	2 cut(s)	592, 1888
BstYI	RGATCY	2 cut(s)	592, 1888
Bsu15I	ATCGAT	1 cut(s)	792
BsuI	GTATCC	1 cut(s)	131
BsuRI	GGCC	6 cut(s)	225, 432, 477, 1653, 1788, 1926
BsuTUI	ATCGAT	1 cut(s)	792
BtgZI	GCGATG	1 cut(s)	783
BtsCI	GGATG	2 cut(s)	51, 207
BtsI	GCAGTG	1 cut(s)	1179
BtsIMutI	CAGTG	5 cut(s)	631, 804, 942, 1179, 1418
BveI	ACCTGC	1 cut(s)	116
Cac8I	GCNNGC	5 cut(s)	11, 924, 1069, 1651, 1835
CaiI	CAGNNNCTG	1 cut(s)	1514
CciI	TCATGA	1 cut(s)	267
CfoI	GCGC	2 cut(s)	246, 305
Cfr10I	RCCGGY	2 cut(s)	1484, 1829
Cfr13I	GGNCC	5 cut(s)	154, 493, 1425, 1651, 1786
ClaI	ATCGAT	1 cut(s)	792
CseI	GACGC	1 cut(s)	241
Csp6I	GTAC	3 cut(s)	277, 1470, 1525
CspAI	ACCGGT	1 cut(s)	1484
CviQI	GTAC	3 cut(s)	277, 1470, 1525
DdeI	CTNAG	6 cut(s)	194, 261, 657, 830, 1235, 1545
DraI	TTTAAA	1 cut(s)	1855
EaeI	YGGCCR	2 cut(s)	223, 430
Eam1104I	CTCTTC	1 cut(s)	1913
EarI	CTCTTC	1 cut(s)	1913
Eco130I	CCWWGG	3 cut(s)	391, 478, 1789
Eco47I	GGWCC	3 cut(s)	154, 493, 1425
Eco57I	CTGAAG	2 cut(s)	240, 1536
Eco88I	CYCGRG	1 cut(s)	1281
EcoNI	CCTNNNNNAGG	1 cut(s)	61
EcoO109I	RGGNCCY	2 cut(s)	154, 493
EcoRI	GAATTC	1 cut(s)	1106
EcoRII	CCWGG	3 cut(s)	667, 1128, 1774
EcoT14I	CCWWGG	3 cut(s)	391, 478, 1789
EcoT22I	ATGCAT	1 cut(s)	924
ErhI	CCWWGG	3 cut(s)	391, 478, 1789
FaqI	GGGAC	1 cut(s)	506
FauI	CCCGC	2 cut(s)	1182, 1642
FauNDI	CATATG	1 cut(s)	1603
FbaI	TGATCA	2 cut(s)	1080, 1864
FblI	GTMKAC	1 cut(s)	444
Fnu4HI	GCNGC	9 cut(s)	96, 99, 123, 301, 522, 723, 744, 1346, 1764
FokI	GGATG	2 cut(s)	58, 214
Fsp4HI	GCNGC	9 cut(s)	96, 99, 123, 301, 522, 723, 744, 1346, 1764
FspBI	CTAG	6 cut(s)	42, 177, 699, 1304, 1748, 1954
GlaI	GCGC	2 cut(s)	245, 304
GluI	GCNGC	9 cut(s)	96, 99, 123, 301, 522, 723, 744, 1346, 1764
GsuI	CTGGAG	1 cut(s)	883
HaeII	RGCGCY	1 cut(s)	306
HaeIII	GGCC	6 cut(s)	225, 432, 477, 1653, 1788, 1926
HapII	CCGG	4 cut(s)	199, 1485, 1710, 1830
HgaI	GACGC	1 cut(s)	241
HhaI	GCGC	2 cut(s)	246, 305
Hin6I	GCGC	2 cut(s)	244, 303
HinP1I	GCGC	2 cut(s)	244, 303
HincII	GTYRAC	1 cut(s)	1246
HindII	GTYRAC	1 cut(s)	1246
HindIII	AAGCTT	1 cut(s)	1835
HinfI	GANTC	9 cut(s)	180, 329, 655, 912, 1219, 1325, 1534, 1595, 1636
HpaII	CCGG	4 cut(s)	199, 1485, 1710, 1830
HphI	GGTGA	7 cut(s)	367, 1011, 1334, 1590, 1631, 1819, 1826
Hpy166II	GTNNAC	3 cut(s)	445, 1246, 1525
Hpy188I	TCNGA	3 cut(s)	322, 660, 991
Hpy188III	TCNNGA	6 cut(s)	268, 699, 916, 1262, 1283, 1538
Hpy8I	GTNNAC	3 cut(s)	445, 1246, 1525
HpyAV	CCTTC	2 cut(s)	863, 1361
HpyCH4III	ACNGT	6 cut(s)	626, 808, 932, 1501, 1625, 1861
HpyCH4IV	ACGT	2 cut(s)	561, 1737
HpyF10VI	GCNNNNNNNGC	7 cut(s)	122, 241, 527, 749, 1286, 1345, 1760
HpyF3I	CTNAG	6 cut(s)	194, 261, 657, 830, 1235, 1545
HpySE526I	ACGT	2 cut(s)	561, 1737
HspAI	GCGC	2 cut(s)	244, 303
KflI	GGGWCCC	1 cut(s)	493
KpnI	GGTACC	1 cut(s)	1473
Ksp22I	TGATCA	2 cut(s)	1080, 1864
LmnI	GCTCC	2 cut(s)	482, 518
Lsp1109I	GCAGC	6 cut(s)	107, 134, 508, 734, 755, 1332
LweI	GCATC	7 cut(s)	409, 798, 909, 1159, 1531, 1636, 1741
MaeI	CTAG	6 cut(s)	42, 177, 699, 1304, 1748, 1954
MaeII	ACGT	2 cut(s)	561, 1737
MaeIII	GTNAC	7 cut(s)	346, 611, 825, 940, 1510, 1619, 1814
MboII	GAAGA	5 cut(s)	65, 641, 1301, 1372, 1900
MflI	RGATCY	2 cut(s)	592, 1888
MhlI	GDGCHC	1 cut(s)	70
MlsI	TGGCCA	2 cut(s)	225, 432
MluCI	AATT	6 cut(s)	18, 861, 1011, 1106, 1161, 1182
MluNI	TGGCCA	2 cut(s)	225, 432
MlyI	GAGTC	3 cut(s)	649, 921, 1630
MmeI	TCCRAC	4 cut(s)	1403, 1570, 1753, 1864
Mox20I	TGGCCA	2 cut(s)	225, 432
Mph1103I	ATGCAT	1 cut(s)	924
MscI	TGGCCA	2 cut(s)	225, 432
MseI	TTAA	7 cut(s)	507, 954, 1194, 1554, 1628, 1656, 1854
MslI	CAYNNNNRTG	5 cut(s)	438, 839, 1076, 1082, 1151
Msp20I	TGGCCA	2 cut(s)	225, 432
MspA1I	CMGCKG	4 cut(s)	98, 260, 725, 903
MspCI	CTTAAG	1 cut(s)	953
MspI	CCGG	4 cut(s)	199, 1485, 1710, 1830
MspR9I	CCNGG	3 cut(s)	669, 1130, 1776
Mva1269I	GAATGC	1 cut(s)	834
MvaI	CCWGG	3 cut(s)	669, 1130, 1776
MwoI	GCNNNNNNNGC	7 cut(s)	122, 241, 527, 749, 1286, 1345, 1760
NdeI	CATATG	1 cut(s)	1603
NlaIV	GGNNCC	5 cut(s)	156, 494, 495, 1089, 1471
NmuCI	GTSAC	3 cut(s)	940, 1619, 1814
NsiI	ATGCAT	1 cut(s)	924
NspI	RCATGY	2 cut(s)	844, 926
PaeI	GCATGC	1 cut(s)	926
PagI	TCATGA	1 cut(s)	267
PaqCI	CACCTGC	1 cut(s)	116
PctI	GAATGC	1 cut(s)	834
PfeI	GAWTC	6 cut(s)	180, 329, 1219, 1325, 1534, 1595
PflMI	CCANNNNNTGG	1 cut(s)	1434
PfoI	TCCNGGA	1 cut(s)	1774
PinAI	ACCGGT	1 cut(s)	1484
PkrI	GCNGC	9 cut(s)	97, 100, 124, 302, 523, 724, 745, 1347, 1765
Ple19I	CGATCG	1 cut(s)	1301
PleI	GAGTC	3 cut(s)	649, 920, 1630
PpsI	GAGTC	3 cut(s)	649, 920, 1630
PpuMI	RGGWCCY	2 cut(s)	154, 493
PsiI	TTATAA	1 cut(s)	1202
Psp5II	RGGWCCY	2 cut(s)	154, 493
Psp6I	CCWGG	3 cut(s)	667, 1128, 1774
PspGI	CCWGG	3 cut(s)	667, 1128, 1774
PspN4I	GGNNCC	5 cut(s)	156, 494, 495, 1089, 1471
PspPI	GGNCC	5 cut(s)	154, 493, 1425, 1651, 1786
PspPPI	RGGWCCY	2 cut(s)	154, 493
PsrI	GAACNNNNNNTAC	2 cut(s)	263, 295
PstI	CTGCAG	2 cut(s)	337, 1333
PstNI	CAGNNNCTG	1 cut(s)	1514
PsuI	RGATCY	2 cut(s)	592, 1888
PvuI	CGATCG	1 cut(s)	1301
PvuII	CAGCTG	3 cut(s)	260, 725, 903
RgaI	GCGATCGC	1 cut(s)	1301
RsaI	GTAC	3 cut(s)	278, 1471, 1526
RsaNI	GTAC	3 cut(s)	277, 1470, 1525
RseI	CAYNNNNRTG	5 cut(s)	438, 839, 1076, 1082, 1151
SaqAI	TTAA	7 cut(s)	507, 954, 1194, 1554, 1628, 1656, 1854
SatI	GCNGC	9 cut(s)	96, 99, 123, 301, 522, 723, 744, 1346, 1764
Sau96I	GGNCC	5 cut(s)	154, 493, 1425, 1651, 1786
ScaI	AGTACT	1 cut(s)	278
SchI	GAGTC	3 cut(s)	649, 921, 1630
ScrFI	CCNGG	3 cut(s)	669, 1130, 1776
SduI	GDGCHC	1 cut(s)	70
SfaAI	GCGATCGC	1 cut(s)	1301
SfaNI	GCATC	7 cut(s)	409, 798, 909, 1159, 1531, 1636, 1741
SfcI	CTRYAG	3 cut(s)	333, 622, 1329
SgfI	GCGATCGC	1 cut(s)	1301
SinI	GGWCC	3 cut(s)	154, 493, 1425
SmiMI	CAYNNNNRTG	5 cut(s)	438, 839, 1076, 1082, 1151
SmlI	CTYRAG	1 cut(s)	953
SmoI	CTYRAG	1 cut(s)	953
SphI	GCATGC	1 cut(s)	926
Sse9I	AATT	6 cut(s)	18, 861, 1011, 1106, 1161, 1182
SsiI	CCGC	6 cut(s)	98, 300, 581, 1189, 1649, 1763
SspMI	CTAG	6 cut(s)	42, 177, 699, 1304, 1748, 1954
StyD4I	CCNGG	3 cut(s)	667, 1128, 1774
StyI	CCWWGG	3 cut(s)	391, 478, 1789
TaaI	ACNGT	6 cut(s)	626, 808, 932, 1501, 1625, 1861
TaiI	ACGT	2 cut(s)	564, 1740
TaqI	TCGA	3 cut(s)	792, 867, 1046
TasI	AATT	6 cut(s)	18, 861, 1011, 1106, 1161, 1182
TatI	WGTACW	2 cut(s)	276, 1524
TauI	GCSGC	3 cut(s)	101, 303, 1766
TfiI	GAWTC	6 cut(s)	180, 329, 1219, 1325, 1534, 1595
Tru1I	TTAA	7 cut(s)	507, 954, 1194, 1554, 1628, 1656, 1854
Tru9I	TTAA	7 cut(s)	507, 954, 1194, 1554, 1628, 1656, 1854
TscAI	CASTG	5 cut(s)	631, 811, 949, 1179, 1418
TseFI	GTSAC	3 cut(s)	940, 1619, 1814
TseI	GCWGC	6 cut(s)	95, 122, 521, 722, 743, 1345
Tsp45I	GTSAC	3 cut(s)	940, 1619, 1814
TspDTI	ATGAA	8 cut(s)	48, 158, 284, 416, 546, 985, 1099, 1892
TspGWI	ACGGA	2 cut(s)	956, 1212
TspRI	CASTG	5 cut(s)	631, 811, 949, 1179, 1418
Van91I	CCANNNNNTGG	1 cut(s)	1434
Vha464I	CTTAAG	1 cut(s)	953
VpaK11BI	GGWCC	3 cut(s)	154, 493, 1425
XagI	CCTNNNNNAGG	1 cut(s)	61
XapI	RAATTY	2 cut(s)	1106, 1182
XbaI	TCTAGA	1 cut(s)	698
XceI	RCATGY	2 cut(s)	844, 926
XmiI	GTMKAC	1 cut(s)	444
XspI	CTAG	6 cut(s)	42, 177, 699, 1304, 1748, 1954
ZrmI	AGTACT	1 cut(s)	278
Zsp2I	ATGCAT	1 cut(s)	924

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.