Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0000924

Physical Location & Seq

Genomic Coordinates Reverse (-)

48663465 .. 48667629

Gene Structure 4165 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG325000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1617 bp

ATGGGGAGTGTTGTGGCTATCTTTCTGGCAGCAGAAGCAGAATACATGGCATACAAGTACCCAAAGAAGTCGATAAATGCTAGAATCAAGGACCTGATGGGCAGAATGACAGTAGCAGAAAAGGTGGGGCAGATGACGCAGCTGGAATGTGGAAATGCAATAGCTGAGATCATGAGGGACTACTCAATAGGCAGTGTACTGAGTGGCGGAGGAAGCATACCGCAGCCACATGCCACTCCACAGGATTGGATCAAAATGTTCAATGACTTTCAAAACGGTTCATTGTCTAGCCACCTTGGAATCCCTATGATTTATGGCATTGATGTTGTTCATGGCCACAATAATGCTTATAAGGCCACCATTTTCCCTCATAATGGATCCAGAACTTGTGAAGAAAATTGGTTCTGCAACTGCTCTCGAATAAAAAATAACCTCACACCTTATAAACTGCACAACAGGTCTGCAAGATGGGGTAGATGTTATGAGAGTTACAGCGAGGATCCTGAAATCGTAAAACAAATGACTGATATCATACTTGGATTGCAAGGCGAAATACCAGCTGATTCACGGAAGGGTGTTCCATTTGTTGGTGGAAAGGATAAGGTAGCGGCTTGCGCAAAACACTTTGTTGGAGACAGTGGTACGATTAAGGGCATCAATGAGAATAATACAGTGATTGATAAGCATGGATTATTGAACATTCACATGCCTGGTTATTACCATTCTATCATCAAGGGCGTATCCACTGTCATGGTTTCCTACTCCAGCTTGAATGGAACGAAGATGCATGCTAACCACAAGCTCATCGCCCAATTTCTTAAGAACACCCTTAAGTTCAGGGGATTTGTCATCTCTGACTGGCAGGGTACCGACAAACTTACCCATCCAGTTCATTCTGATTACCCAAAATCAGTTCTTGCTGGTATTCAAGCTGGCATTGACATGGTCATGGTTCCCTTTAATCATACTGAGTTCATTGGGATTCTAACCAATCATGTCAACAACAAACTCGTCCCAATGAGTCGTATTGATGATGCAGTTGGGAGAATTTTGAGAGTCAAGTTCACTATGGGTCTGTTTGAGAACCCCTTGGCTGATGAGAGCTTTGTTGACCCTTGGAAGCCAGGAGCCACCATCTTAAGTGCCATCACAGCTGCAGTTGACCCCAGCACCGAAATCATCTTCAGTGAAAGTCCTGGTGCTGATTTTGTCACGGAAAACAACTTCTCCTATGCCATTGTTGTTGTTGGAGAGCTCCCTTATGCGGAGACAAACCGGGACAGTTTAAATTTGACAATTGCACAACAGGGTCCAAGCACCATCACCAATGTATGTGGCAAAGTCAAGAGTGTGGTTGTAGTAATCTCTGGCCGTCCTGTTGTGATCGAACCCTACATTTCATTGATGGATGCTCTTGTGGCTGCATGGCTTCCTGGAACTGAAGGTCAAGGAGTTACAGATGTTCTCTTTGGCGATTATGGATTTACTGGCAAGCTTCCTAGGACTTGGTTCAAGACAGTAGATCAGATCCCTATGAATGTTGGTGACAAACATTATGATCCCCTCTTTCCATTTGGTTTTGGCCTTACAACTCAACCGATTAAGAAGTCAAGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0000323 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005886 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0008422 GO:0009056 GO:0009057 GO:0009251 GO:0015926 GO:0016020 GO:0016052 GO:0016787 GO:0016798 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0071704 GO:0071944 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00460 ko00500 ko00940 ko01100 ko01110 map00460 map00500 map00940 map01100 map01110

Pfam Domains

Protein Families

Glyco_hydro_3 Glyco_hydro_3_C

Protein Analysis

538

Amino Acids

59.03

Weight (kDa)

6.99

Isoelectric Point (pI)

31.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_3	PF00933	37 - 125	1.4e-07	Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3	PF00933	152 - 353	1.3e-34	Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C	PF01915	378 - 530	7.6e-26	Glycosyl hydrolase family 3 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000924)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04885 AT5G04885 AT5G04885
fragaria_vesca	FvH4_1g28352 FvH4_1g28352 FvH4_1g28352 FvH4_4g26180 FvH4_4g26180 FvH4_7g05220 FvH4_7g05220
malus_domestica	MD02G1259600.v1.1 MD07G1060900.v1.1 MD11G1177800.v1.1 MD11G1177900.v1.1 MD14G1004200.v1.1
prunus_persica	Prupe.2G065700_v2.0.a1 Prupe.2G065700_v2.0.a1 Prupe.2G065700_v2.0.a1 Prupe.2G065700_v2.0.a1 Prupe.2G065700_v2.0.a1 Prupe.6G150000_v2.0.a1 Prupe.6G150200_v2.0.a1 Prupe.6G150400_v2.0.a1 Prupe.6G150500_v2.0.a1 Prupe.7G007200_v2.0.a1
pyrus_communis	pycom14g00250
rosa_chinensis	RchiOBHm_Chr1g0331601 RchiOBHm_Chr3g0496681 RchiOBHm_Chr3g0496721 RchiOBHm_Chr4g0433771
rosa_laevigata	RLG00000006732 RLG00000022510 RLG00000022511 RLG00000029727 RLG00000034619
rosa_multiflora	Rmu_sc0005044.1_g000017 Rmu_sc0006176.1_g000006
rosa_roxburghii	Rroxscaffold_4G00319030 Rroxscaffold_5G00375200 Rroxscaffold_6G00388790 Rroxscaffold_6G00388830 Rroxscaffold_6G00388850
rosa_rugosa	Rorug01G0095000 Rorug01G0095100 Rorug01G0095200 Rorug03G0185800 Rorug03G0186000 Rorug03G0285500 Rorug03G0285700 Rorug04G0271300 Rorug05G0248600 Rorug06G0034700
rosa_samantha	Rh1AG118800 Rh1CG113800 Rh3AG330600 Rh3BG366600 Rh3CG363300 Rh3CG363500 Rh4AG326000 Rh4CG349100 Rh5AG325000
rosa_wichuraiana	Rw1G009560 Rw3G028940 Rw3G028950

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	351, 444
Acc16I	TGCGCA	1 cut(s)	616
Acc65I	GGTACC	1 cut(s)	866
AccB1I	GGYRCC	1 cut(s)	866
AccB7I	CCANNNNNTGG	1 cut(s)	587
AciI	CCGC	4 cut(s)	207, 221, 608, 1265
AclWI	GGATC	7 cut(s)	257, 372, 385, 494, 507, 1522, 1553
AcoI	YGGCCR	2 cut(s)	334, 1369
AcsI	RAATTY	2 cut(s)	1047, 1288
AcuI	CTGAAG	2 cut(s)	1168, 1461
AfaI	GTAC	4 cut(s)	59, 198, 643, 868
AfiI	CCNNNNNNNGG	3 cut(s)	374, 587, 1264
AflII	CTTAAG	3 cut(s)	818, 830, 1138
AgsI	TTSAA	6 cut(s)	262, 272, 697, 772, 929, 1513
AjnI	CCWGG	4 cut(s)	709, 1123, 1195, 1432
Alw21I	GWGCWC	1 cut(s)	1257
Alw26I	GTCTC	2 cut(s)	627, 1262
AlwI	GGATC	7 cut(s)	257, 372, 385, 494, 507, 1522, 1553
AoxI	GGCC	4 cut(s)	334, 354, 1369, 1582
ApeKI	GCWGC	5 cut(s)	29, 139, 223, 1154, 1421
ApoI	RAATTY	2 cut(s)	1047, 1288
Asp718I	GGTACC	1 cut(s)	866
AspA2I	CCTAGG	1 cut(s)	1499
AspLEI	GCGC	1 cut(s)	617
AspS9I	GGNCC	2 cut(s)	91, 1310
AsuC2I	CCSGG	1 cut(s)	1277
AsuHPI	GGTGA	2 cut(s)	1315, 1556
AvaII	GGWCC	2 cut(s)	91, 1310
AvrII	CCTAGG	1 cut(s)	1499
BalI	TGGCCA	1 cut(s)	336
BamHI	GGATCC	2 cut(s)	377, 499
BanI	GGYRCC	1 cut(s)	866
BanII	GRGCYC	1 cut(s)	1257
Bbv12I	GWGCWC	1 cut(s)	1257
BbvI	GCAGC	5 cut(s)	41, 151, 235, 1141, 1408
BccI	CCATC	7 cut(s)	91, 462, 891, 1142, 1154, 1328, 1399
BceAI	ACGGC	1 cut(s)	1356
BciT130I	CCWGG	4 cut(s)	711, 1125, 1197, 1434
BciVI	GTATCC	1 cut(s)	751
BcnI	CCSGG	1 cut(s)	1277
BcoDI	GTCTC	2 cut(s)	627, 1262
BfaI	CTAG	3 cut(s)	81, 288, 1500
BfmI	CTRYAG	1 cut(s)	1155
BfrI	CTTAAG	3 cut(s)	818, 830, 1138
BfuI	GTATCC	1 cut(s)	751
BisI	GCNGC	6 cut(s)	30, 140, 224, 609, 1155, 1422
BlnI	CCTAGG	1 cut(s)	1499
BlsI	GCNGC	6 cut(s)	31, 141, 225, 610, 1156, 1423
Bme1390I	CCNGG	5 cut(s)	711, 1125, 1197, 1277, 1434
Bme18I	GGWCC	2 cut(s)	91, 1310
BmgT120I	GGNCC	2 cut(s)	91, 1310
BmiI	GGNNCC	6 cut(s)	379, 501, 868, 954, 1129, 1311
BmrFI	CCNGG	5 cut(s)	711, 1125, 1197, 1277, 1434
BmsI	GCATC	4 cut(s)	663, 774, 1024, 1399
BpmI	CTGGAG	1 cut(s)	748
BpuMI	CCSGG	1 cut(s)	1277
BsaJI	CCNNGG	4 cut(s)	295, 1089, 1115, 1499
BsaXI	ACNNNNNCTCC	2 cut(s)	1211, 1241
Bsc4I	CCNNNNNNNGG	3 cut(s)	374, 587, 1264
Bse1I	ACTGG	3 cut(s)	863, 887, 1492
BseBI	CCWGG	4 cut(s)	711, 1125, 1197, 1434
BseDI	CCNNGG	4 cut(s)	295, 1089, 1115, 1499
BseGI	GGATG	2 cut(s)	883, 1414
BseLI	CCNNNNNNNGG	3 cut(s)	374, 587, 1264
BseMII	CTCAG	3 cut(s)	156, 191, 960
BseNI	ACTGG	3 cut(s)	863, 887, 1492
BseXI	GCAGC	5 cut(s)	41, 151, 235, 1141, 1408
BseYI	CCCAGC	1 cut(s)	1166
BsgI	GTGCAG	1 cut(s)	434
BshFI	GGCC	4 cut(s)	336, 356, 1371, 1584
BshNI	GGYRCC	1 cut(s)	866
BsiHKAI	GWGCWC	1 cut(s)	1257
BsiSI	CCGG	1 cut(s)	1276
BslFI	GGGAC	3 cut(s)	191, 998, 1292
BslI	CCNNNNNNNGG	3 cut(s)	374, 587, 1264
BsmAI	GTCTC	2 cut(s)	627, 1262
BsmFI	GGGAC	3 cut(s)	191, 998, 1292
BsnI	GGCC	4 cut(s)	336, 356, 1371, 1584
Bsp1286I	GDGCHC	1 cut(s)	1257
Bsp143I	GATC	8 cut(s)	168, 249, 377, 499, 1383, 1522, 1527, 1558
BspACI	CCGC	4 cut(s)	207, 221, 608, 1265
BspANI	GGCC	4 cut(s)	336, 356, 1371, 1584
BspCNI	CTCAG	3 cut(s)	157, 192, 961
BspHI	TCATGA	1 cut(s)	171
BspLI	GGNNCC	6 cut(s)	379, 501, 868, 954, 1129, 1311
BspMAI	CTGCAG	1 cut(s)	1159
BspPI	GGATC	7 cut(s)	257, 372, 385, 494, 507, 1522, 1553
BspT107I	GGYRCC	1 cut(s)	866
BspTI	CTTAAG	3 cut(s)	818, 830, 1138
BsrI	ACTGG	3 cut(s)	863, 887, 1492
BssECI	CCNNGG	4 cut(s)	295, 1089, 1115, 1499
BssMI	GATC	8 cut(s)	168, 249, 377, 499, 1383, 1522, 1527, 1558
BssT1I	CCWWGG	4 cut(s)	295, 1089, 1115, 1499
Bst2UI	CCWGG	4 cut(s)	711, 1125, 1197, 1434
Bst4CI	ACNGT	7 cut(s)	112, 278, 638, 673, 748, 1283, 1519
BstAFI	CTTAAG	3 cut(s)	818, 830, 1138
BstC8I	GCNNGC	4 cut(s)	613, 789, 934, 1493
BstDEI	CTNAG	3 cut(s)	165, 200, 969
BstF5I	GGATG	2 cut(s)	883, 1414
BstHHI	GCGC	1 cut(s)	617
BstKTI	GATC	8 cut(s)	171, 252, 380, 502, 1386, 1525, 1530, 1561
BstMAI	GTCTC	2 cut(s)	627, 1262
BstMBI	GATC	8 cut(s)	168, 249, 377, 499, 1383, 1522, 1527, 1558
BstMWI	GCNNNNNNNGC	7 cut(s)	35, 136, 213, 353, 614, 1151, 1418
BstNI	CCWGG	4 cut(s)	711, 1125, 1197, 1434
BstNSI	RCATGY	3 cut(s)	233, 709, 791
BstSCI	CCNGG	5 cut(s)	709, 1123, 1195, 1275, 1432
BstSFI	CTRYAG	1 cut(s)	1155
BstV1I	GCAGC	5 cut(s)	41, 151, 235, 1141, 1408
BstX2I	RGATCY	3 cut(s)	377, 499, 1527
BstXI	CCANNNNNNTGG	2 cut(s)	246, 751
BstYI	RGATCY	3 cut(s)	377, 499, 1527
BsuI	GTATCC	1 cut(s)	751
BsuRI	GGCC	4 cut(s)	336, 356, 1371, 1584
BtgZI	GCGATG	1 cut(s)	790
BtsCI	GGATG	2 cut(s)	883, 1414
BtsI	GCAGTG	1 cut(s)	199
BtsIMutI	CAGTG	5 cut(s)	199, 643, 678, 744, 1192
Cac8I	GCNNGC	4 cut(s)	613, 789, 934, 1493
CciI	TCATGA	1 cut(s)	171
CfoI	GCGC	1 cut(s)	617
Cfr13I	GGNCC	2 cut(s)	91, 1310
CseI	GACGC	1 cut(s)	145
Csp6I	GTAC	4 cut(s)	58, 197, 642, 867
CspCI	CAANNNNNGTGG	2 cut(s)	1315, 1350
CviQI	GTAC	4 cut(s)	58, 197, 642, 867
DdeI	CTNAG	3 cut(s)	165, 200, 969
DpnI	GATC	8 cut(s)	170, 251, 379, 501, 1385, 1524, 1529, 1560
DpnII	GATC	8 cut(s)	168, 249, 377, 499, 1383, 1522, 1527, 1558
DraI	TTTAAA	1 cut(s)	1287
EaeI	YGGCCR	2 cut(s)	334, 1369
EciI	GGCGGA	1 cut(s)	222
Ecl136II	GAGCTC	1 cut(s)	1255
Eco130I	CCWWGG	4 cut(s)	295, 1089, 1115, 1499
Eco24I	GRGCYC	1 cut(s)	1257
Eco32I	GATATC	1 cut(s)	529
Eco47I	GGWCC	2 cut(s)	91, 1310
Eco53kI	GAGCTC	1 cut(s)	1255
Eco57I	CTGAAG	2 cut(s)	1168, 1461
EcoICRI	GAGCTC	1 cut(s)	1255
EcoO109I	RGGNCCY	1 cut(s)	91
EcoRII	CCWGG	4 cut(s)	709, 1123, 1195, 1432
EcoRV	GATATC	1 cut(s)	529
EcoT14I	CCWWGG	4 cut(s)	295, 1089, 1115, 1499
EcoT22I	ATGCAT	1 cut(s)	789
EcoT38I	GRGCYC	1 cut(s)	1257
ErhI	CCWWGG	4 cut(s)	295, 1089, 1115, 1499
FaqI	GGGAC	3 cut(s)	191, 998, 1292
Fnu4HI	GCNGC	6 cut(s)	30, 140, 224, 609, 1155, 1422
FokI	GGATG	2 cut(s)	870, 1421
FriOI	GRGCYC	1 cut(s)	1257
Fsp4HI	GCNGC	6 cut(s)	30, 140, 224, 609, 1155, 1422
FspBI	CTAG	3 cut(s)	81, 288, 1500
FspI	TGCGCA	1 cut(s)	616
GlaI	GCGC	1 cut(s)	616
GluI	GCNGC	6 cut(s)	30, 140, 224, 609, 1155, 1422
GsaI	CCCAGC	1 cut(s)	1170
GsuI	CTGGAG	1 cut(s)	748
HaeIII	GGCC	4 cut(s)	336, 356, 1371, 1584
HapII	CCGG	1 cut(s)	1276
HgaI	GACGC	1 cut(s)	145
HhaI	GCGC	1 cut(s)	617
Hin6I	GCGC	1 cut(s)	615
HinP1I	GCGC	1 cut(s)	615
HincII	GTYRAC	3 cut(s)	1000, 1111, 1162
HindII	GTYRAC	3 cut(s)	1000, 1111, 1162
HindIII	AAGCTT	1 cut(s)	1493
HinfI	GANTC	6 cut(s)	84, 300, 563, 982, 1021, 1056
HpaII	CCGG	1 cut(s)	1276
HphI	GGTGA	2 cut(s)	1315, 1556
Hpy166II	GTNNAC	5 cut(s)	197, 1000, 1065, 1111, 1162
Hpy188I	TCNGA	3 cut(s)	856, 898, 1527
Hpy188III	TCNNGA	6 cut(s)	172, 381, 417, 503, 1345, 1513
Hpy8I	GTNNAC	5 cut(s)	197, 1000, 1065, 1111, 1162
HpyAV	CCTTC	2 cut(s)	565, 1436
HpyCH4III	ACNGT	7 cut(s)	112, 278, 638, 673, 748, 1283, 1519
HpyF10VI	GCNNNNNNNGC	7 cut(s)	35, 136, 213, 353, 614, 1151, 1418
HpyF3I	CTNAG	3 cut(s)	165, 200, 969
HspAI	GCGC	1 cut(s)	615
KpnI	GGTACC	1 cut(s)	870
Kzo9I	GATC	8 cut(s)	168, 249, 377, 499, 1383, 1522, 1527, 1558
LmnI	GCTCC	2 cut(s)	1127, 1260
Lsp1109I	GCAGC	5 cut(s)	41, 151, 235, 1141, 1408
LweI	GCATC	4 cut(s)	663, 774, 1024, 1399
MaeI	CTAG	3 cut(s)	81, 288, 1500
MaeIII	GTNAC	4 cut(s)	488, 1210, 1453, 1544
MalI	GATC	8 cut(s)	170, 251, 379, 501, 1385, 1524, 1529, 1560
MboI	GATC	8 cut(s)	168, 249, 377, 499, 1383, 1522, 1527, 1558
MboII	GAAGA	3 cut(s)	404, 793, 1174
MfeI	CAATTG	1 cut(s)	1296
MflI	RGATCY	3 cut(s)	377, 499, 1527
MhlI	GDGCHC	1 cut(s)	1257
MlsI	TGGCCA	1 cut(s)	336
MluCI	AATT	5 cut(s)	397, 812, 1047, 1288, 1296
MluNI	TGGCCA	1 cut(s)	336
MlyI	GAGTC	2 cut(s)	1030, 1065
MmeI	TCCRAC	2 cut(s)	610, 1228
MnlI	CCTC	6 cut(s)	168, 203, 378, 443, 490, 1574
Mox20I	TGGCCA	1 cut(s)	336
Mph1103I	ATGCAT	1 cut(s)	789
MscI	TGGCCA	1 cut(s)	336
MseI	TTAA	7 cut(s)	648, 819, 831, 960, 1139, 1286, 1602
MslI	CAYNNNNRTG	5 cut(s)	342, 704, 749, 941, 947
Msp20I	TGGCCA	1 cut(s)	336
MspA1I	CMGCKG	3 cut(s)	142, 560, 1154
MspCI	CTTAAG	3 cut(s)	818, 830, 1138
MspI	CCGG	1 cut(s)	1276
MspR9I	CCNGG	5 cut(s)	711, 1125, 1197, 1277, 1434
MunI	CAATTG	1 cut(s)	1296
MvaI	CCWGG	4 cut(s)	711, 1125, 1197, 1434
MwoI	GCNNNNNNNGC	7 cut(s)	35, 136, 213, 353, 614, 1151, 1418
NciI	CCSGG	1 cut(s)	1277
NdeII	GATC	8 cut(s)	168, 249, 377, 499, 1383, 1522, 1527, 1558
NlaIV	GGNNCC	6 cut(s)	379, 501, 868, 954, 1129, 1311
NmuCI	GTSAC	2 cut(s)	1210, 1544
NsbI	TGCGCA	1 cut(s)	616
NsiI	ATGCAT	1 cut(s)	789
NspI	RCATGY	3 cut(s)	233, 709, 791
PaeI	GCATGC	1 cut(s)	791
PagI	TCATGA	1 cut(s)	171
PfeI	GAWTC	4 cut(s)	84, 300, 563, 982
PflFI	GACNNNGTC	1 cut(s)	944
PflMI	CCANNNNNTGG	1 cut(s)	587
PfoI	TCCNGGA	1 cut(s)	1432
PkrI	GCNGC	6 cut(s)	31, 141, 225, 610, 1156, 1423
PleI	GAGTC	2 cut(s)	1029, 1064
PpsI	GAGTC	2 cut(s)	1029, 1064
PpuMI	RGGWCCY	1 cut(s)	91
PsiI	TTATAA	2 cut(s)	351, 444
Psp124BI	GAGCTC	1 cut(s)	1257
Psp5II	RGGWCCY	1 cut(s)	91
Psp6I	CCWGG	4 cut(s)	709, 1123, 1195, 1432
PspFI	CCCAGC	1 cut(s)	1166
PspGI	CCWGG	4 cut(s)	709, 1123, 1195, 1432
PspN4I	GGNNCC	6 cut(s)	379, 501, 868, 954, 1129, 1311
PspPI	GGNCC	2 cut(s)	91, 1310
PspPPI	RGGWCCY	1 cut(s)	91
PstI	CTGCAG	1 cut(s)	1159
PsuI	RGATCY	3 cut(s)	377, 499, 1527
PsyI	GACNNNGTC	1 cut(s)	944
PvuII	CAGCTG	3 cut(s)	142, 560, 1154
RsaI	GTAC	4 cut(s)	59, 198, 643, 868
RsaNI	GTAC	4 cut(s)	58, 197, 642, 867
RseI	CAYNNNNRTG	5 cut(s)	342, 704, 749, 941, 947
SacI	GAGCTC	1 cut(s)	1257
SaqAI	TTAA	7 cut(s)	648, 819, 831, 960, 1139, 1286, 1602
SatI	GCNGC	6 cut(s)	30, 140, 224, 609, 1155, 1422
Sau3AI	GATC	8 cut(s)	168, 249, 377, 499, 1383, 1522, 1527, 1558
Sau96I	GGNCC	2 cut(s)	91, 1310
SchI	GAGTC	2 cut(s)	1030, 1065
ScrFI	CCNGG	5 cut(s)	711, 1125, 1197, 1277, 1434
SduI	GDGCHC	1 cut(s)	1257
SfaNI	GCATC	4 cut(s)	663, 774, 1024, 1399
SfcI	CTRYAG	1 cut(s)	1155
SinI	GGWCC	2 cut(s)	91, 1310
SmiMI	CAYNNNNRTG	5 cut(s)	342, 704, 749, 941, 947
SmlI	CTYRAG	3 cut(s)	818, 830, 1138
SmoI	CTYRAG	3 cut(s)	818, 830, 1138
SphI	GCATGC	1 cut(s)	791
Sse9I	AATT	5 cut(s)	397, 812, 1047, 1288, 1296
SsiI	CCGC	4 cut(s)	207, 221, 608, 1265
SspMI	CTAG	3 cut(s)	81, 288, 1500
SstI	GAGCTC	1 cut(s)	1257
StyD4I	CCNGG	5 cut(s)	709, 1123, 1195, 1275, 1432
StyI	CCWWGG	4 cut(s)	295, 1089, 1115, 1499
TaaI	ACNGT	7 cut(s)	112, 278, 638, 673, 748, 1283, 1519
TaqI	TCGA	3 cut(s)	71, 418, 1386
TasI	AATT	5 cut(s)	397, 812, 1047, 1288, 1296
TatI	WGTACW	1 cut(s)	196
TauI	GCSGC	1 cut(s)	611
TfiI	GAWTC	4 cut(s)	84, 300, 563, 982
Tru1I	TTAA	7 cut(s)	648, 819, 831, 960, 1139, 1286, 1602
Tru9I	TTAA	7 cut(s)	648, 819, 831, 960, 1139, 1286, 1602
TscAI	CASTG	5 cut(s)	199, 643, 678, 751, 1192
TseFI	GTSAC	2 cut(s)	1210, 1544
TseI	GCWGC	5 cut(s)	29, 139, 223, 1154, 1421
Tsp45I	GTSAC	2 cut(s)	1210, 1544
TspDTI	ATGAA	6 cut(s)	270, 320, 881, 964, 1389, 1550
TspGWI	ACGGA	2 cut(s)	583, 1229
TspRI	CASTG	5 cut(s)	199, 643, 678, 751, 1192
Tth111I	GACNNNGTC	1 cut(s)	944
Van91I	CCANNNNNTGG	1 cut(s)	587
Vha464I	CTTAAG	3 cut(s)	818, 830, 1138
VpaK11BI	GGWCC	2 cut(s)	91, 1310
XapI	RAATTY	2 cut(s)	1047, 1288
XceI	RCATGY	3 cut(s)	233, 709, 791
XmaJI	CCTAGG	1 cut(s)	1499
XspI	CTAG	3 cut(s)	81, 288, 1500
Zsp2I	ATGCAT	1 cut(s)	789

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.