Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr1

Orthogroup ID

OG0000924

Physical Location & Seq

Genomic Coordinates Forward (+)

21017033 .. 21022542

Gene Structure 5510 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw1G009560.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1992 bp

ATGGCTAGGGTTTCATTTTCGGTGGTAGCTATGTTGCTGCTTTGGTGGTGGGTGGCTATGGCGAAGCTGAAGGTGAAGGCGGAGTACATCAAGTACAAAGATCCAAACCAACCGGCCGCCGCTCGAGTTGGGGACCTTCTTAGCAGAATGACTCTTGAAGAGAAAATTGGTCAGATGGTGCAGATTGATAGAAGTGTTGCCAATGTCAACACCATGAAAACTTACTTCATTGGGAGTGTGTTGAGCGGCGGTGGTAGTGCACCACTTCCAGAGGCCAGTGCCGAGGACTGGGTTAACATGATCAACGGATTTCAGAAGGGGGCTCTAGCTAGCCGTTTGGGGATTCCCATGATTTATGGCATTGACGCTGTTCATGGACACAATAACGTCTTTAATGCTACAATATTTCCGCATAATGTTGGGCTTGGAGCAACCAGGGATCCTGAACTGGCGAAAAGAATTGGTGCTGCAACTGCTCTTGAAGTCCGAGCTACGGGCATTCCTTATGCATTTGCTCCATGCATTGCGGTATGTAGAGATCCACGATGGGGTCGCTGTTATGAAAGCTACAGCGAGGATCACAAAATTGTGCAAGCAATGACAGAAATTATTCCAGGTTTACAGGGAGATATTCCTGCTAATTCTCGGAAAGGAGTTCCATATGTTGGCGGAAATAAAAAGGTTGCTGCTTGTGCAAAGCATTTTGTGGGTGATGGTGGGACAAGCAAGGGCATTAATGAGAACAACACCGTTATTGACCGACATGATTTGCTTAGCATCCACATGCCTGCTTATTCTGATTCGATTATCAAGGGTGTCTCCACAGTGATGATTTCGTACTCCAGTTGGAATGGGGAGAAGATGCATGCAAACCGTGACCTAGTCACTGGCTTCCTTAAAGAGACCCTTAAGTTTAAGGGTTTTGTTATATCAGACTGGGAGGGAATTGACAGGATTACCTCGCCACCACATTCAAACTACACATACTCTGTCCAAGCTTCAATTCTTGCTGGAATAGACATGGTAATGGTTCCTGTTAACTATAAAGAGTTCATCAATGATCTCACCTACCTGATCAAGACCAATGTTATCCCAATGGATCGTATTGATGATGCTGTCGCAAGGATTTTGCTTGTCAAGTTTACCATGGGTCTTTTTGAGAACCCTTTGGGTGATCTCAGCCTAGTCAATGAGCTTGGAAGCCAGGCTCATAGAGATTTGGCAAGGGAGGCTGTCAGAAAGTCACTTGTGCTTCTGAAGAATGGGAAAAATGGAACCAATCCGGTCCTACCTCTTCCTAAGAAGGTGCCCAAAATCTTGGTTGCTGGCAGTCATGCTGATAATCTGGGTTACCAGTGTGGTGGATGGACAATTGCTTGGCAAGGATTCAGCGGCAACAACTTTACAAGGGGAACTACCGTTCTTGGTGCTATCAAATCAACAGTTGATTCAAGCACAGAAGTTATCTATCATGAGAATCCTGATGGTGAATTTGTTAAGTCCAACAACTTTGAATATGCCATTGTTGTTGTTGGCGAGCACCCTTATGCCGAGACTTATGGAGACAGCATGAACTTAACTATGGCGGATCCTGGCCCAAGTGTCATCACCAGTGTGTGTGAAGCTGTCAAGTGCATTGTTATTATAATTTCTGGCAGACCTGTTGTGATCGAACCATATGTTTCCTCAATTGATGCCCTGGTGGCAGCATGGTTGCCAGGAACTGAAGGCCAAGGCATTACTGATGTCCTTTATGGCGAGTATGGATTCAGCGGAAAGCTTTCAAGAACATGGTTCAGAAGTGTAGATCAACTTCCAATGAATGTTGGGGATCCACACTATGATCCACTTTTCCCTTTTGGGTTTGGACTTGAAACTGAGTCAGTTAAGGAGCTCGTAGGAAGGTCAACTTCAGCTGGTGTATTTGGAAGGCCATGCACACTTCTCATTGTCGTTGCTCTAATTCTCAGCTTCAGAGAAGAGTTTCTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0000323 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005886 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0008422 GO:0009056 GO:0009057 GO:0009251 GO:0015926 GO:0016020 GO:0016052 GO:0016787 GO:0016798 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0071704 GO:0071944 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00460 ko00500 ko00940 ko01100 ko01110 map00460 map00500 map00940 map01100 map01110

Pfam Domains

Protein Families

Glyco_hydro_3 Glyco_hydro_3_C

Protein Analysis

663

Amino Acids

72.24

Weight (kDa)

6.3

Isoelectric Point (pI)

25.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_3	PF00933	51 - 377	5.2e-68	Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C	PF01915	416 - 625	1.1e-36	Glycosyl hydrolase family 3 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000924)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04885 AT5G04885 AT5G04885
fragaria_vesca	FvH4_1g28352 FvH4_1g28352 FvH4_1g28352 FvH4_4g26180 FvH4_4g26180 FvH4_7g05220 FvH4_7g05220
malus_domestica	MD02G1259600.v1.1 MD07G1060900.v1.1 MD11G1177800.v1.1 MD11G1177900.v1.1 MD14G1004200.v1.1
prunus_persica	Prupe.2G065700_v2.0.a1 Prupe.2G065700_v2.0.a1 Prupe.2G065700_v2.0.a1 Prupe.2G065700_v2.0.a1 Prupe.2G065700_v2.0.a1 Prupe.6G150000_v2.0.a1 Prupe.6G150200_v2.0.a1 Prupe.6G150400_v2.0.a1 Prupe.6G150500_v2.0.a1 Prupe.7G007200_v2.0.a1
pyrus_communis	pycom14g00250
rosa_chinensis	RchiOBHm_Chr1g0331601 RchiOBHm_Chr3g0496681 RchiOBHm_Chr3g0496721 RchiOBHm_Chr4g0433771
rosa_laevigata	RLG00000006732 RLG00000022510 RLG00000022511 RLG00000029727 RLG00000034619
rosa_multiflora	Rmu_sc0005044.1_g000017 Rmu_sc0006176.1_g000006
rosa_roxburghii	Rroxscaffold_4G00319030 Rroxscaffold_5G00375200 Rroxscaffold_6G00388790 Rroxscaffold_6G00388830 Rroxscaffold_6G00388850
rosa_rugosa	Rorug01G0095000 Rorug01G0095100 Rorug01G0095200 Rorug03G0185800 Rorug03G0186000 Rorug03G0285500 Rorug03G0285700 Rorug04G0271300 Rorug05G0248600 Rorug06G0034700
rosa_samantha	Rh1AG118800 Rh1CG113800 Rh3AG330600 Rh3BG366600 Rh3CG363300 Rh3CG363500 Rh4AG326000 Rh4CG349100 Rh5AG325000
rosa_wichuraiana	Rw1G009560 Rw3G028940 Rw3G028950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1646
AccB1I	GGYRCC	1 cut(s)	1306
AccB7I	CCANNNNNTGG	1 cut(s)	665
AccBSI	CCGCTC	2 cut(s)	122, 246
AcoI	YGGCCR	1 cut(s)	114
AcsI	RAATTY	1 cut(s)	1490
AcuI	CTGAAG	5 cut(s)	89, 1277, 1746, 1896, 1957
AfaI	GTAC	3 cut(s)	86, 95, 839
AfiI	CCNNNNNNNGG	4 cut(s)	288, 493, 548, 665
AflII	CTTAAG	1 cut(s)	908
AgsI	TTSAA	8 cut(s)	158, 482, 975, 1002, 1452, 1514, 1785, 1874
AjnI	CCWGG	6 cut(s)	434, 613, 1203, 1591, 1698, 1717
AjuI	GAANNNNNNNTTGG	4 cut(s)	150, 182, 987, 1019
AleI	CACNNNNGTG	1 cut(s)	1613
AloI	GAACNNNNNNTCC	2 cut(s)	1404, 1436
Alw21I	GWGCWC	3 cut(s)	262, 1542, 1896
Alw26I	GTCTC	4 cut(s)	823, 896, 1547, 1557
Alw44I	GTGCAC	1 cut(s)	258
AlwNI	CAGNNNCTG	1 cut(s)	1724
Ama87I	CYCGRG	1 cut(s)	123
AoxI	GGCC	5 cut(s)	114, 273, 1594, 1729, 1931
ApaLI	GTGCAC	1 cut(s)	258
ApeKI	GCWGC	4 cut(s)	37, 467, 686, 1706
ApoI	RAATTY	1 cut(s)	1490
AseI	ATTAAT	1 cut(s)	735
Asp700I	GAANNNNTTC	3 cut(s)	609, 1780, 1983
AspS9I	GGNCC	3 cut(s)	133, 1285, 1595
AsuHPI	GGTGA	6 cut(s)	85, 722, 1057, 1184, 1499, 1600
AsuNHI	GCTAGC	1 cut(s)	329
AvaI	CYCGRG	1 cut(s)	123
AvaII	GGWCC	2 cut(s)	133, 1285
BaeGI	GKGCMC	2 cut(s)	262, 1311
BamHI	GGATCC	3 cut(s)	439, 1588, 1831
BanI	GGYRCC	1 cut(s)	1306
BanII	GRGCYC	2 cut(s)	325, 1896
BarI	GAAGNNNNNNTAC	4 cut(s)	68, 100, 1797, 1829
Bbv12I	GWGCWC	3 cut(s)	262, 1542, 1896
BbvI	GCAGC	4 cut(s)	24, 454, 673, 1718
BccI	CCATC	5 cut(s)	169, 540, 707, 1359, 1478
BceAI	ACGGC	1 cut(s)	318
BciT130I	CCWGG	6 cut(s)	436, 615, 1205, 1593, 1700, 1719
BclI	TGATCA	2 cut(s)	300, 1074
BcoDI	GTCTC	4 cut(s)	823, 896, 1547, 1557
BfaI	CTAG	6 cut(s)	6, 326, 330, 881, 1184, 1990
BfmI	CTRYAG	1 cut(s)	568
BfrI	CTTAAG	1 cut(s)	908
BglI	GCCNNNNNGGC	1 cut(s)	1703
BisI	GCNGC	8 cut(s)	38, 117, 120, 247, 468, 687, 1393, 1707
BlpI	GCTNAGC	1 cut(s)	773
BlsI	GCNGC	8 cut(s)	39, 118, 121, 248, 469, 688, 1394, 1708
Bme1390I	CCNGG	6 cut(s)	436, 615, 1205, 1593, 1700, 1719
Bme18I	GGWCC	2 cut(s)	133, 1285
BmeT110I	CYCGRG	1 cut(s)	123
BmgT120I	GGNCC	3 cut(s)	133, 1285, 1595
BmiI	GGNNCC	7 cut(s)	134, 441, 1032, 1276, 1308, 1590, 1833
BmrFI	CCNGG	6 cut(s)	436, 615, 1205, 1593, 1700, 1719
BmrI	ACTGGG	2 cut(s)	298, 946
BmsI	GCATC	4 cut(s)	786, 852, 1102, 1684
BmtI	GCTAGC	1 cut(s)	333
BmuI	ACTGGG	2 cut(s)	298, 946
BpmI	CTGGAG	1 cut(s)	826
Bpu1102I	GCTNAGC	1 cut(s)	773
BsaI	GGTCTC	1 cut(s)	896
BsaJI	CCNNGG	5 cut(s)	282, 435, 1146, 1698, 1732
BsaWI	WCCGGW	1 cut(s)	1282
Bsc4I	CCNNNNNNNGG	4 cut(s)	288, 493, 548, 665
Bse118I	RCCGGY	1 cut(s)	112
Bse1I	ACTGG	8 cut(s)	276, 293, 453, 843, 892, 941, 1354, 1611
Bse3DI	GCAATG	2 cut(s)	522, 603
BseBI	CCWGG	6 cut(s)	436, 615, 1205, 1593, 1700, 1719
BseDI	CCNNGG	5 cut(s)	282, 435, 1146, 1698, 1732
BseGI	GGATG	2 cut(s)	777, 1370
BseLI	CCNNNNNNNGG	4 cut(s)	288, 493, 548, 665
BseMI	GCAATG	2 cut(s)	522, 603
BseMII	CTCAG	3 cut(s)	1192, 1869, 1981
BseNI	ACTGG	8 cut(s)	276, 293, 453, 843, 892, 941, 1354, 1611
BseSI	GKGCMC	2 cut(s)	262, 1311
BseX3I	CGGCCG	1 cut(s)	114
BseXI	GCAGC	4 cut(s)	24, 454, 673, 1718
BsgI	GTGCAG	1 cut(s)	200
Bsh1285I	CGRYCG	1 cut(s)	117
BshFI	GGCC	5 cut(s)	116, 275, 1596, 1731, 1933
BshNI	GGYRCC	1 cut(s)	1306
BsiEI	CGRYCG	1 cut(s)	117
BsiHKAI	GWGCWC	3 cut(s)	262, 1542, 1896
BsiHKCI	CYCGRG	1 cut(s)	123
BsiSI	CCGG	2 cut(s)	113, 1283
BslFI	GGGAC	2 cut(s)	146, 733
BslI	CCNNNNNNNGG	4 cut(s)	288, 493, 548, 665
BsmAI	GTCTC	4 cut(s)	823, 896, 1547, 1557
BsmFI	GGGAC	2 cut(s)	146, 733
BsmI	GAATGC	1 cut(s)	498
BsnI	GGCC	5 cut(s)	116, 275, 1596, 1731, 1933
Bso31I	GGTCTC	1 cut(s)	896
BsoBI	CYCGRG	1 cut(s)	123
Bsp1286I	GDGCHC	5 cut(s)	262, 325, 1311, 1542, 1896
Bsp1720I	GCTNAGC	1 cut(s)	773
Bsp19I	CCATGG	1 cut(s)	1146
BspANI	GGCC	5 cut(s)	116, 275, 1596, 1731, 1933
BspCNI	CTCAG	3 cut(s)	1191, 1870, 1980
BspHI	TCATGA	1 cut(s)	1471
BspLI	GGNNCC	7 cut(s)	134, 441, 1032, 1276, 1308, 1590, 1833
BspOI	GCTAGC	1 cut(s)	333
BspT107I	GGYRCC	1 cut(s)	1306
BspTI	CTTAAG	1 cut(s)	908
BspTNI	GGTCTC	1 cut(s)	896
BsrBI	CCGCTC	2 cut(s)	122, 246
BsrDI	GCAATG	2 cut(s)	522, 603
BsrFI	RCCGGY	1 cut(s)	112
BsrI	ACTGG	8 cut(s)	276, 293, 453, 843, 892, 941, 1354, 1611
BssAI	RCCGGY	1 cut(s)	112
BssECI	CCNNGG	5 cut(s)	282, 435, 1146, 1698, 1732
BssT1I	CCWWGG	2 cut(s)	1146, 1732
Bst2UI	CCWGG	6 cut(s)	436, 615, 1205, 1593, 1700, 1719
Bst4CI	ACNGT	5 cut(s)	751, 826, 875, 1420, 1444
Bst6I	CTCTTC	3 cut(s)	153, 1299, 1974
BstAFI	CTTAAG	1 cut(s)	908
BstC8I	GCNNGC	6 cut(s)	331, 594, 789, 867, 1327, 1538
BstDEI	CTNAG	6 cut(s)	140, 773, 1178, 1299, 1878, 1967
BstDSI	CCRYGG	1 cut(s)	1146
BstEII	GGTNACC	1 cut(s)	1349
BstF5I	GGATG	2 cut(s)	777, 1370
BstMAI	GTCTC	4 cut(s)	823, 896, 1547, 1557
BstMCI	CGRYCG	1 cut(s)	117
BstMWI	GCNNNNNNNGC	4 cut(s)	473, 692, 1229, 1703
BstNI	CCWGG	6 cut(s)	436, 615, 1205, 1593, 1700, 1719
BstNSI	RCATGY	2 cut(s)	787, 869
BstPI	GGTNACC	1 cut(s)	1349
BstSCI	CCNGG	6 cut(s)	434, 613, 1203, 1591, 1698, 1717
BstSFI	CTRYAG	1 cut(s)	568
BstSLI	GKGCMC	2 cut(s)	262, 1311
BstV1I	GCAGC	4 cut(s)	24, 454, 673, 1718
BstX2I	RGATCY	5 cut(s)	100, 439, 538, 1588, 1831
BstXI	CCANNNNNNTGG	2 cut(s)	1318, 1361
BstYI	RGATCY	5 cut(s)	100, 439, 538, 1588, 1831
BstZI	CGGCCG	1 cut(s)	114
BsuRI	GGCC	5 cut(s)	116, 275, 1596, 1731, 1933
BtgI	CCRYGG	1 cut(s)	1146
BtsCI	GGATG	2 cut(s)	777, 1370
BtsIMutI	CAGTG	5 cut(s)	283, 831, 885, 1361, 1618
Cac8I	GCNNGC	6 cut(s)	331, 594, 789, 867, 1327, 1538
CaiI	CAGNNNCTG	1 cut(s)	1724
CciI	TCATGA	1 cut(s)	1471
Cfr10I	RCCGGY	1 cut(s)	112
Cfr13I	GGNCC	3 cut(s)	133, 1285, 1595
CseI	GACGC	1 cut(s)	374
Csp6I	GTAC	3 cut(s)	85, 94, 838
CviQI	GTAC	3 cut(s)	85, 94, 838
DdeI	CTNAG	6 cut(s)	140, 773, 1178, 1299, 1878, 1967
EaeI	YGGCCR	1 cut(s)	114
EagI	CGGCCG	1 cut(s)	114
Eam1104I	CTCTTC	3 cut(s)	153, 1299, 1974
EarI	CTCTTC	3 cut(s)	153, 1299, 1974
EciI	GGCGGA	3 cut(s)	95, 684, 1601
Ecl136II	GAGCTC	1 cut(s)	1894
EclXI	CGGCCG	1 cut(s)	114
Eco130I	CCWWGG	2 cut(s)	1146, 1732
Eco24I	GRGCYC	2 cut(s)	325, 1896
Eco31I	GGTCTC	1 cut(s)	896
Eco47I	GGWCC	2 cut(s)	133, 1285
Eco52I	CGGCCG	1 cut(s)	114
Eco53kI	GAGCTC	1 cut(s)	1894
Eco57I	CTGAAG	5 cut(s)	89, 1277, 1746, 1896, 1957
Eco88I	CYCGRG	1 cut(s)	123
Eco91I	GGTNACC	1 cut(s)	1349
EcoICRI	GAGCTC	1 cut(s)	1894
EcoO109I	RGGNCCY	1 cut(s)	133
EcoO65I	GGTNACC	1 cut(s)	1349
EcoRII	CCWGG	6 cut(s)	434, 613, 1203, 1591, 1698, 1717
EcoT14I	CCWWGG	2 cut(s)	1146, 1732
EcoT22I	ATGCAT	3 cut(s)	511, 524, 867
EcoT38I	GRGCYC	2 cut(s)	325, 1896
ErhI	CCWWGG	2 cut(s)	1146, 1732
FalI	AAGNNNNNCTT	4 cut(s)	891, 923, 1894, 1926
FaqI	GGGAC	2 cut(s)	146, 733
FauNDI	CATATG	2 cut(s)	661, 1678
FbaI	TGATCA	2 cut(s)	300, 1074
Fnu4HI	GCNGC	8 cut(s)	38, 117, 120, 247, 468, 687, 1393, 1707
FokI	GGATG	2 cut(s)	764, 1377
FriOI	GRGCYC	2 cut(s)	325, 1896
Fsp4HI	GCNGC	8 cut(s)	38, 117, 120, 247, 468, 687, 1393, 1707
FspBI	CTAG	6 cut(s)	6, 326, 330, 881, 1184, 1990
GluI	GCNGC	8 cut(s)	38, 117, 120, 247, 468, 687, 1393, 1707
GsuI	CTGGAG	1 cut(s)	826
HaeIII	GGCC	5 cut(s)	116, 275, 1596, 1731, 1933
HapII	CCGG	2 cut(s)	113, 1283
HgaI	GACGC	1 cut(s)	374
HincII	GTYRAC	4 cut(s)	208, 295, 1039, 1908
HindII	GTYRAC	4 cut(s)	208, 295, 1039, 1908
HindIII	AAGCTT	2 cut(s)	996, 1778
HinfI	GANTC	8 cut(s)	151, 343, 800, 1386, 1448, 1477, 1767, 1880
HpaI	GTTAAC	2 cut(s)	295, 1039
HpaII	CCGG	2 cut(s)	113, 1283
HphI	GGTGA	6 cut(s)	85, 722, 1057, 1184, 1499, 1600
Hpy166II	GTNNAC	7 cut(s)	208, 260, 295, 620, 1039, 1143, 1908
Hpy188III	TCNNGA	8 cut(s)	155, 269, 443, 479, 1078, 1472, 1481, 1785
Hpy8I	GTNNAC	7 cut(s)	208, 260, 295, 620, 1039, 1143, 1908
HpyAV	CCTTC	8 cut(s)	64, 70, 146, 310, 1297, 1721, 1896, 1923
HpyCH4III	ACNGT	5 cut(s)	751, 826, 875, 1420, 1444
HpyCH4IV	ACGT	1 cut(s)	387
HpyF10VI	GCNNNNNNNGC	4 cut(s)	473, 692, 1229, 1703
HpyF3I	CTNAG	6 cut(s)	140, 773, 1178, 1299, 1878, 1967
HpySE526I	ACGT	1 cut(s)	387
Ksp22I	TGATCA	2 cut(s)	300, 1074
KspAI	GTTAAC	2 cut(s)	295, 1039
LmnI	GCTCC	3 cut(s)	428, 520, 1891
Lsp1109I	GCAGC	4 cut(s)	24, 454, 673, 1718
LweI	GCATC	4 cut(s)	786, 852, 1102, 1684
MaeI	CTAG	6 cut(s)	6, 326, 330, 881, 1184, 1990
MaeII	ACGT	1 cut(s)	387
MaeIII	GTNAC	4 cut(s)	875, 883, 1242, 1349
MbiI	CCGCTC	2 cut(s)	122, 246
MboII	GAAGA	5 cut(s)	170, 871, 1270, 1286, 1991
MfeI	CAATTG	2 cut(s)	1371, 1689
MflI	RGATCY	5 cut(s)	100, 439, 538, 1588, 1831
MhlI	GDGCHC	5 cut(s)	262, 325, 1311, 1542, 1896
MlyI	GAGTC	2 cut(s)	145, 1889
MmeI	TCCRAC	2 cut(s)	827, 1527
MnlI	CCTC	8 cut(s)	265, 277, 568, 934, 970, 1222, 1302, 1696
Mph1103I	ATGCAT	3 cut(s)	511, 524, 867
MroXI	GAANNNNTTC	3 cut(s)	609, 1780, 1983
MslI	CAYNNNNRTG	5 cut(s)	782, 827, 1025, 1545, 1613
MspA1I	CMGCKG	3 cut(s)	1392, 1773, 1916
MspCI	CTTAAG	1 cut(s)	908
MspI	CCGG	2 cut(s)	113, 1283
MspR9I	CCNGG	6 cut(s)	436, 615, 1205, 1593, 1700, 1719
MunI	CAATTG	2 cut(s)	1371, 1689
Mva1269I	GAATGC	1 cut(s)	498
MvaI	CCWGG	6 cut(s)	436, 615, 1205, 1593, 1700, 1719
MwoI	GCNNNNNNNGC	4 cut(s)	473, 692, 1229, 1703
NcoI	CCATGG	1 cut(s)	1146
NdeI	CATATG	2 cut(s)	661, 1678
NheI	GCTAGC	1 cut(s)	329
NlaIV	GGNNCC	7 cut(s)	134, 441, 1032, 1276, 1308, 1590, 1833
NmeAIII	GCCGAG	2 cut(s)	307, 1576
NmuCI	GTSAC	3 cut(s)	875, 883, 1242
NsiI	ATGCAT	3 cut(s)	511, 524, 867
NspI	RCATGY	2 cut(s)	787, 869
OliI	CACNNNNGTG	1 cut(s)	1613
PaeI	GCATGC	1 cut(s)	869
PaeR7I	CTCGAG	1 cut(s)	123
PagI	TCATGA	1 cut(s)	1471
PctI	GAATGC	1 cut(s)	498
PdmI	GAANNNNTTC	3 cut(s)	609, 1780, 1983
PfeI	GAWTC	6 cut(s)	343, 800, 1386, 1448, 1477, 1767
PflFI	GACNNNGTC	1 cut(s)	881
PflMI	CCANNNNNTGG	1 cut(s)	665
PkrI	GCNGC	8 cut(s)	39, 118, 121, 248, 469, 688, 1394, 1708
PleI	GAGTC	2 cut(s)	145, 1888
PpsI	GAGTC	2 cut(s)	145, 1888
PpuMI	RGGWCCY	1 cut(s)	133
PshBI	ATTAAT	1 cut(s)	735
PsiI	TTATAA	1 cut(s)	1646
Psp124BI	GAGCTC	1 cut(s)	1896
Psp5II	RGGWCCY	1 cut(s)	133
Psp6I	CCWGG	6 cut(s)	434, 613, 1203, 1591, 1698, 1717
PspEI	GGTNACC	1 cut(s)	1349
PspGI	CCWGG	6 cut(s)	434, 613, 1203, 1591, 1698, 1717
PspN4I	GGNNCC	7 cut(s)	134, 441, 1032, 1276, 1308, 1590, 1833
PspPI	GGNCC	3 cut(s)	133, 1285, 1595
PspPPI	RGGWCCY	1 cut(s)	133
PspXI	VCTCGAGB	1 cut(s)	123
PstNI	CAGNNNCTG	1 cut(s)	1724
PsuI	RGATCY	5 cut(s)	100, 439, 538, 1588, 1831
PsyI	GACNNNGTC	1 cut(s)	881
PvuII	CAGCTG	1 cut(s)	1916
RsaI	GTAC	3 cut(s)	86, 95, 839
RsaNI	GTAC	3 cut(s)	85, 94, 838
RseI	CAYNNNNRTG	5 cut(s)	782, 827, 1025, 1545, 1613
SacI	GAGCTC	1 cut(s)	1896
SatI	GCNGC	8 cut(s)	38, 117, 120, 247, 468, 687, 1393, 1707
Sau96I	GGNCC	3 cut(s)	133, 1285, 1595
SchI	GAGTC	2 cut(s)	145, 1889
ScrFI	CCNGG	6 cut(s)	436, 615, 1205, 1593, 1700, 1719
SduI	GDGCHC	5 cut(s)	262, 325, 1311, 1542, 1896
SfaNI	GCATC	4 cut(s)	786, 852, 1102, 1684
SfcI	CTRYAG	1 cut(s)	568
Sfr274I	CTCGAG	1 cut(s)	123
SinI	GGWCC	2 cut(s)	133, 1285
SlaI	CTCGAG	1 cut(s)	123
SmiMI	CAYNNNNRTG	5 cut(s)	782, 827, 1025, 1545, 1613
SmlI	CTYRAG	2 cut(s)	123, 908
SmoI	CTYRAG	2 cut(s)	123, 908
SphI	GCATGC	1 cut(s)	869
SspI	AATATT	1 cut(s)	405
SspMI	CTAG	6 cut(s)	6, 326, 330, 881, 1184, 1990
SstI	GAGCTC	1 cut(s)	1896
StyD4I	CCNGG	6 cut(s)	434, 613, 1203, 1591, 1698, 1717
StyI	CCWWGG	2 cut(s)	1146, 1732
TaaI	ACNGT	5 cut(s)	751, 826, 875, 1420, 1444
TaiI	ACGT	1 cut(s)	390
TaqI	TCGA	3 cut(s)	124, 803, 1671
TaqII	GACCGA	1 cut(s)	774
TatI	WGTACW	2 cut(s)	84, 93
TauI	GCSGC	4 cut(s)	119, 122, 249, 1395
TfiI	GAWTC	6 cut(s)	343, 800, 1386, 1448, 1477, 1767
TscAI	CASTG	5 cut(s)	283, 831, 892, 1361, 1618
TseFI	GTSAC	3 cut(s)	875, 883, 1242
TseI	GCWGC	4 cut(s)	37, 467, 686, 1706
Tsp45I	GTSAC	3 cut(s)	875, 883, 1242
TspDTI	ATGAA	7 cut(s)	217, 230, 362, 576, 1042, 1586, 1835
TspGWI	ACGGA	1 cut(s)	321
TspRI	CASTG	5 cut(s)	283, 831, 892, 1361, 1618
Tth111I	GACNNNGTC	1 cut(s)	881
Van91I	CCANNNNNTGG	1 cut(s)	665
Vha464I	CTTAAG	1 cut(s)	908
VneI	GTGCAC	1 cut(s)	258
VpaK11BI	GGWCC	2 cut(s)	133, 1285
VspI	ATTAAT	1 cut(s)	735
XapI	RAATTY	1 cut(s)	1490
XceI	RCATGY	2 cut(s)	787, 869
XhoI	CTCGAG	1 cut(s)	123
XmnI	GAANNNNTTC	3 cut(s)	609, 1780, 1983
XspI	CTAG	6 cut(s)	6, 326, 330, 881, 1184, 1990
Zsp2I	ATGCAT	3 cut(s)	511, 524, 867

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.