Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0000924

Physical Location & Seq

Genomic Coordinates Reverse (-)

5692068 .. 5698954

Gene Structure 6887 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1060900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1977 bp

ATGGCTTGGGTTTCGTTACCATTGGTGGGTTTGTTGTTGGTTTGGTGGTGGATGGGTTTTGTAACGGTGAAAGCCGATTACATCAAGTACAAAGATCCATCCCAACCACCGGCTGCTCGAGTTGGAGACCTTCTTAGTAGAATGACTCTTGAAGAAAAGATTGGTCAGATGGTGCAGATTGATAGGAGTGTTGCCTCTGTTGATACTATGAAAACTTACTCCATTGGTAGTGTATTGAGCGGTGGTGGCAGTGCACCACTTCCAGAAGCTAGTGCTGAAGACTGGGTTAACATGATTAATGAATTTCAGAAGGGAGCTCTATCTAGTCGTTTGGGCATTCCAATGATCTATGGCATTGATGCTGTTCATGGACACAATAATGTCTATAATGCTACAATATTTCCGCATAATGTTGGGCTTGGAGCTACCAGGGACCCTGATCTGGTACGAAGAATTGGTTCTGCAACTGCTCTTGAAGCCCGAGCTACTGGGATTCCTTATGTATTTGCTCCATGCATTGCGGTATGTAGAGATCCAAGATGGGGTCGATGTTATGAAAGCTACAGTGAAGATCACAAAATTGTGGAGGAAATGACAGAGATTATTCCAGGTTTGCAGGGAGATATCCCTGCTAATTCTCGTAAAGGAGTGCCATATGTTGGTGGAAAGAAAAAGGTTGCAGCTTGTGCAAAGCACTTTGTCGGTGATGGTGGGACAAGCAGGGGCATTAATGAGAACAACACTGTGACTGACCGGCACGAGTTGCTTAGCATCCACATGCCTGCCTATTCTGATTCGATCATCAAGGGCGTCTCAACCGTGATGATTTCCTACTCCAGTTGGAATGGGGATAAGATGCATGCAAACCGTGATCTAATTACGAACTTCCTCAAGGGCACCCTTAAATTTAAGGGTTTTGTTATATCGGATTGGGAGGGTATTGACAGGATTACCTCGCCACCACATTCAAACTACACATACTCTGTCCAAGCTTCAGTTCTAGCTGGAATTGACATGGTCATGGTCCCTTTCAATTATAAAGAGTTCATTAATGATCTCATTAACCTTGTCAAGACTAACGTGGTTCCAATGGATCGTATTGATGATGCTGTGGGAAGGATTCTACTTGTCAAGTTCACCATGGGTCTCTTTGAGAACCCTTTGGCTGATCTCAGCCTAGTCAATGAGCTTGGAAGCCAGGCTCATAGAGATTTGGCAAGGGAGGCTGTCAGAAAATCCCTTGTGCTTTTGAAGAATGGGAAAAGCAAGAACAATACAGATCCCGTCATACCTCTTCCCAAGAAGGTGTCCAAAATTCTAGTTGCCGGCGGTCATGCTGATAATCTGGGTTACCAATGCGGTGGATGGACAATGGCATGGCAAGGATTCAGTGGCAACAACTATACAAGCGGGACTACGATTCTTGGTGCTATAAACTCAACAGTTGACTCAAGCACAGAAGTTGTCTACAGAGAAAATCCTGATGGCAATTTTGTTAAGTCCAACAACTTTGCATATGCTATTGTTGTGGTTGGTGAGCACCCTTATGCTGAGACTTTGGGAGACAGCACAAATCTAACAATGGCGGAACCTGGCCCTAGTGTCATCAGCAATGTGTGTGAAAGTGTCAAGTGCATTGTTATCTTAATTACTGGCAGACCTATCGTAATTGAACCATACATTTCCTCGATTGATGCTCTGGTGGCAGCATGGTTGCCAGGCTCTGAAGGCCAAGGTATTACTGATGTCCTTTTTGGCGATCATGGATTCAGCGGAAAGCTTGCAAGGACATGGTTCAGAACTGTAGATCAACTCCCAATGAATGTTGGGGATTCACACTATGATCCACTTTTCCCGTTAGGGCATGGACTTGCAACTGAGTCAGTCAAGCAGCTTGTAGCGAGGTCAACCTCAGCTGGTGCTGTTGCAAGGCCATGCATATTTGTCGTCGTGGTTGCTCTAATTCTGAGCTTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0000323 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005886 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0008422 GO:0009056 GO:0009057 GO:0009251 GO:0015926 GO:0016020 GO:0016052 GO:0016787 GO:0016798 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0071704 GO:0071944 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00460 ko00500 ko00940 ko01100 ko01110 map00460 map00500 map00940 map01100 map01110

Pfam Domains

Protein Families

Glyco_hydro_3 Glyco_hydro_3_C

Protein Analysis

659

Amino Acids

71.34

Weight (kDa)

6.22

Isoelectric Point (pI)

25.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_3	PF00933	49 - 375	1.1e-66	Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C	PF01915	415 - 624	3.2e-34	Glycosyl hydrolase family 3 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000924)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04885 AT5G04885 AT5G04885
fragaria_vesca	FvH4_1g28352 FvH4_1g28352 FvH4_1g28352 FvH4_4g26180 FvH4_4g26180 FvH4_7g05220 FvH4_7g05220
malus_domestica	MD02G1259600.v1.1 MD07G1060900.v1.1 MD11G1177800.v1.1 MD11G1177900.v1.1 MD14G1004200.v1.1
prunus_persica	Prupe.2G065700_v2.0.a1 Prupe.2G065700_v2.0.a1 Prupe.2G065700_v2.0.a1 Prupe.2G065700_v2.0.a1 Prupe.2G065700_v2.0.a1 Prupe.6G150000_v2.0.a1 Prupe.6G150200_v2.0.a1 Prupe.6G150400_v2.0.a1 Prupe.6G150500_v2.0.a1 Prupe.7G007200_v2.0.a1
pyrus_communis	pycom14g00250
rosa_chinensis	RchiOBHm_Chr1g0331601 RchiOBHm_Chr3g0496681 RchiOBHm_Chr3g0496721 RchiOBHm_Chr4g0433771
rosa_laevigata	RLG00000006732 RLG00000022510 RLG00000022511 RLG00000029727 RLG00000034619
rosa_multiflora	Rmu_sc0005044.1_g000017 Rmu_sc0006176.1_g000006
rosa_roxburghii	Rroxscaffold_4G00319030 Rroxscaffold_5G00375200 Rroxscaffold_6G00388790 Rroxscaffold_6G00388830 Rroxscaffold_6G00388850
rosa_rugosa	Rorug01G0095000 Rorug01G0095100 Rorug01G0095200 Rorug03G0185800 Rorug03G0186000 Rorug03G0285500 Rorug03G0285700 Rorug04G0271300 Rorug05G0248600 Rorug06G0034700
rosa_samantha	Rh1AG118800 Rh1CG113800 Rh3AG330600 Rh3BG366600 Rh3CG363300 Rh3CG363500 Rh4AG326000 Rh4CG349100 Rh5AG325000
rosa_wichuraiana	Rw1G009560 Rw3G028940 Rw3G028950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1038
AccB1I	GGYRCC	1 cut(s)	896
AccB7I	CCANNNNNTGG	1 cut(s)	659
AccBSI	CCGCTC	1 cut(s)	240
AccI	GTMKAC	1 cut(s)	1467
AciI	CCGC	8 cut(s)	240, 404, 521, 1329, 1359, 1410, 1586, 1773
AclWI	GGATC	5 cut(s)	89, 527, 1101, 1274, 1838
AcsI	RAATTY	3 cut(s)	302, 905, 1314
AcuI	CTGAAG	3 cut(s)	297, 978, 1746
AcyI	GRCGYC	1 cut(s)	810
AfaI	GTAC	2 cut(s)	89, 447
AfiI	CCNNNNNNNGG	5 cut(s)	26, 109, 442, 542, 659
AgsI	TTSAA	6 cut(s)	152, 476, 969, 1033, 1252, 1673
AjnI	CCWGG	5 cut(s)	428, 607, 1197, 1591, 1717
AjuI	GAANNNNNNNTTGG	4 cut(s)	144, 176, 981, 1013
Alw21I	GWGCWC	3 cut(s)	256, 319, 1542
Alw26I	GTCTC	5 cut(s)	120, 817, 1151, 1547, 1557
Alw44I	GTGCAC	1 cut(s)	252
AlwI	GGATC	5 cut(s)	89, 527, 1101, 1274, 1838
AlwNI	CAGNNNCTG	1 cut(s)	1724
Ama87I	CYCGRG	2 cut(s)	117, 480
AoxI	GGCC	3 cut(s)	1594, 1729, 1931
ApaLI	GTGCAC	1 cut(s)	252
ApeKI	GCWGC	4 cut(s)	113, 680, 1706, 1891
ApoI	RAATTY	3 cut(s)	302, 905, 1314
AseI	ATTAAT	3 cut(s)	297, 729, 1050
Asp700I	GAANNNNTTC	1 cut(s)	457
AspS9I	GGNCC	3 cut(s)	433, 1024, 1595
AsuHPI	GGTGA	4 cut(s)	79, 716, 1129, 1547
AvaI	CYCGRG	2 cut(s)	117, 480
AvaII	GGWCC	2 cut(s)	433, 1024
BaeGI	GKGCMC	2 cut(s)	256, 899
BanI	GGYRCC	1 cut(s)	896
BanII	GRGCYC	1 cut(s)	319
BauI	CACGAG	1 cut(s)	758
BbsI	GAAGAC	1 cut(s)	285
Bbv12I	GWGCWC	3 cut(s)	256, 319, 1542
BbvCI	CCTCAGC	1 cut(s)	1912
BbvI	GCAGC	4 cut(s)	100, 692, 1718, 1903
BccI	CCATC	7 cut(s)	46, 106, 163, 534, 701, 1359, 1478
BcgI	CGANNNNNNTGC	4 cut(s)	1645, 1679, 1927, 1961
BciT130I	CCWGG	5 cut(s)	430, 609, 1199, 1593, 1719
BcoDI	GTCTC	5 cut(s)	120, 817, 1151, 1547, 1557
BfaI	CTAG	6 cut(s)	270, 324, 1001, 1178, 1319, 1599
BfmI	CTRYAG	3 cut(s)	562, 1468, 1803
BisI	GCNGC	4 cut(s)	114, 681, 1707, 1892
BlpI	GCTNAGC	1 cut(s)	767
BlsI	GCNGC	4 cut(s)	115, 682, 1708, 1893
Bme1390I	CCNGG	5 cut(s)	430, 609, 1199, 1593, 1719
Bme18I	GGWCC	2 cut(s)	433, 1024
BmeT110I	CYCGRG	2 cut(s)	117, 480
BmgT120I	GGNCC	3 cut(s)	433, 1024, 1595
BmiI	GGNNCC	6 cut(s)	434, 435, 898, 1026, 1086, 1590
BmrFI	CCNGG	5 cut(s)	430, 609, 1199, 1593, 1719
BmrI	ACTGGG	2 cut(s)	292, 498
BmsI	GCATC	5 cut(s)	349, 780, 846, 1096, 1684
BmuI	ACTGGG	2 cut(s)	292, 498
BpiI	GAAGAC	1 cut(s)	285
BpmI	CTGGAG	1 cut(s)	820
Bpu10I	CCTNAGC	1 cut(s)	1912
Bpu1102I	GCTNAGC	1 cut(s)	767
BpuEI	CTTGAG	2 cut(s)	875, 1435
BsaHI	GRCGYC	1 cut(s)	810
BsaI	GGTCTC	2 cut(s)	120, 1151
BsaJI	CCNNGG	3 cut(s)	429, 1140, 1732
Bsc4I	CCNNNNNNNGG	5 cut(s)	26, 109, 442, 542, 659
Bse118I	RCCGGY	3 cut(s)	109, 753, 1325
Bse1I	ACTGG	4 cut(s)	287, 493, 837, 1657
Bse3DI	GCAATG	2 cut(s)	516, 1618
BseBI	CCWGG	5 cut(s)	430, 609, 1199, 1593, 1719
BseDI	CCNNGG	3 cut(s)	429, 1140, 1732
BseGI	GGATG	4 cut(s)	57, 98, 771, 1370
BseLI	CCNNNNNNNGG	5 cut(s)	26, 109, 442, 542, 659
BseMI	GCAATG	2 cut(s)	516, 1618
BseMII	CTCAG	5 cut(s)	1186, 1542, 1869, 1926, 1958
BseNI	ACTGG	4 cut(s)	287, 493, 837, 1657
BseSI	GKGCMC	2 cut(s)	256, 899
BseXI	GCAGC	4 cut(s)	100, 692, 1718, 1903
BsgI	GTGCAG	1 cut(s)	194
BshFI	GGCC	3 cut(s)	1596, 1731, 1933
BshNI	GGYRCC	1 cut(s)	896
BsiHKAI	GWGCWC	3 cut(s)	256, 319, 1542
BsiHKCI	CYCGRG	2 cut(s)	117, 480
BsiSI	CCGG	3 cut(s)	110, 754, 1326
BslFI	GGGAC	4 cut(s)	446, 727, 1010, 1426
BslI	CCNNNNNNNGG	5 cut(s)	26, 109, 442, 542, 659
BsmAI	GTCTC	5 cut(s)	120, 817, 1151, 1547, 1557
BsmBI	CGTCTC	1 cut(s)	817
BsmFI	GGGAC	4 cut(s)	446, 727, 1010, 1426
BsmI	GAATGC	1 cut(s)	336
BsnI	GGCC	3 cut(s)	1596, 1731, 1933
Bso31I	GGTCTC	2 cut(s)	120, 1151
BsoBI	CYCGRG	2 cut(s)	117, 480
Bsp1286I	GDGCHC	4 cut(s)	256, 319, 899, 1542
Bsp1720I	GCTNAGC	1 cut(s)	767
Bsp19I	CCATGG	1 cut(s)	1140
BspACI	CCGC	8 cut(s)	240, 404, 521, 1329, 1359, 1410, 1586, 1773
BspANI	GGCC	3 cut(s)	1596, 1731, 1933
BspCNI	CTCAG	5 cut(s)	1185, 1543, 1870, 1925, 1959
BspLI	GGNNCC	6 cut(s)	434, 435, 898, 1026, 1086, 1590
BspPI	GGATC	5 cut(s)	89, 527, 1101, 1274, 1838
BspT107I	GGYRCC	1 cut(s)	896
BspTNI	GGTCTC	2 cut(s)	120, 1151
BsrBI	CCGCTC	1 cut(s)	240
BsrDI	GCAATG	2 cut(s)	516, 1618
BsrFI	RCCGGY	3 cut(s)	109, 753, 1325
BsrI	ACTGG	4 cut(s)	287, 493, 837, 1657
BssAI	RCCGGY	3 cut(s)	109, 753, 1325
BssECI	CCNNGG	3 cut(s)	429, 1140, 1732
BssNI	GRCGYC	1 cut(s)	810
BssSI	CACGAG	1 cut(s)	758
BssT1I	CCWWGG	2 cut(s)	1140, 1732
Bst2BI	CACGAG	1 cut(s)	758
Bst2UI	CCWGG	5 cut(s)	430, 609, 1199, 1593, 1719
Bst4CI	ACNGT	7 cut(s)	67, 566, 745, 820, 869, 1444, 1804
Bst6I	CTCTTC	1 cut(s)	1299
BstACI	GRCGYC	1 cut(s)	810
BstAPI	GCANNNNNTGC	2 cut(s)	686, 763
BstC8I	GCNNGC	4 cut(s)	783, 861, 1327, 1782
BstDEI	CTNAG	7 cut(s)	134, 767, 1172, 1551, 1878, 1912, 1967
BstDSI	CCRYGG	1 cut(s)	1140
BstEII	GGTNACC	1 cut(s)	1349
BstF5I	GGATG	4 cut(s)	57, 98, 771, 1370
BstMAI	GTCTC	5 cut(s)	120, 817, 1151, 1547, 1557
BstMWI	GCNNNNNNNGC	7 cut(s)	246, 476, 686, 763, 1223, 1703, 1728
BstNI	CCWGG	5 cut(s)	430, 609, 1199, 1593, 1719
BstNSI	RCATGY	2 cut(s)	781, 863
BstPI	GGTNACC	1 cut(s)	1349
BstSCI	CCNGG	5 cut(s)	428, 607, 1197, 1591, 1717
BstSFI	CTRYAG	3 cut(s)	562, 1468, 1803
BstSLI	GKGCMC	2 cut(s)	256, 899
BstV1I	GCAGC	4 cut(s)	100, 692, 1718, 1903
BstV2I	GAAGAC	1 cut(s)	285
BstX2I	RGATCY	3 cut(s)	94, 532, 1279
BstXI	CCANNNNNNTGG	1 cut(s)	1361
BstYI	RGATCY	3 cut(s)	94, 532, 1279
BsuRI	GGCC	3 cut(s)	1596, 1731, 1933
BtgI	CCRYGG	1 cut(s)	1140
BtsCI	GGATG	4 cut(s)	57, 98, 771, 1370
BtsI	GCAGTG	1 cut(s)	256
BtsIMutI	CAGTG	4 cut(s)	256, 571, 741, 1396
Cac8I	GCNNGC	4 cut(s)	783, 861, 1327, 1782
CaiI	CAGNNNCTG	1 cut(s)	1724
Cfr10I	RCCGGY	3 cut(s)	109, 753, 1325
Cfr13I	GGNCC	3 cut(s)	433, 1024, 1595
CseI	GACGC	1 cut(s)	799
Csp6I	GTAC	2 cut(s)	88, 446
CviQI	GTAC	2 cut(s)	88, 446
DdeI	CTNAG	7 cut(s)	134, 767, 1172, 1551, 1878, 1912, 1967
Eam1104I	CTCTTC	1 cut(s)	1299
EarI	CTCTTC	1 cut(s)	1299
EciI	GGCGGA	1 cut(s)	1601
Ecl136II	GAGCTC	1 cut(s)	317
Eco130I	CCWWGG	2 cut(s)	1140, 1732
Eco24I	GRGCYC	1 cut(s)	319
Eco31I	GGTCTC	2 cut(s)	120, 1151
Eco32I	GATATC	1 cut(s)	625
Eco47I	GGWCC	2 cut(s)	433, 1024
Eco53kI	GAGCTC	1 cut(s)	317
Eco57I	CTGAAG	3 cut(s)	297, 978, 1746
Eco88I	CYCGRG	2 cut(s)	117, 480
Eco91I	GGTNACC	1 cut(s)	1349
EcoICRI	GAGCTC	1 cut(s)	317
EcoO109I	RGGNCCY	1 cut(s)	433
EcoO65I	GGTNACC	1 cut(s)	1349
EcoRII	CCWGG	5 cut(s)	428, 607, 1197, 1591, 1717
EcoRV	GATATC	1 cut(s)	625
EcoT14I	CCWWGG	2 cut(s)	1140, 1732
EcoT22I	ATGCAT	3 cut(s)	518, 861, 1940
EcoT38I	GRGCYC	1 cut(s)	319
ErhI	CCWWGG	2 cut(s)	1140, 1732
Esp3I	CGTCTC	1 cut(s)	817
FaqI	GGGAC	4 cut(s)	446, 727, 1010, 1426
FauI	CCCGC	1 cut(s)	1403
FauNDI	CATATG	2 cut(s)	655, 1516
FblI	GTMKAC	1 cut(s)	1467
Fnu4HI	GCNGC	4 cut(s)	114, 681, 1707, 1892
FokI	GGATG	4 cut(s)	64, 85, 758, 1377
FriOI	GRGCYC	1 cut(s)	319
Fsp4HI	GCNGC	4 cut(s)	114, 681, 1707, 1892
FspBI	CTAG	6 cut(s)	270, 324, 1001, 1178, 1319, 1599
GluI	GCNGC	4 cut(s)	114, 681, 1707, 1892
GsuI	CTGGAG	1 cut(s)	820
HaeIII	GGCC	3 cut(s)	1596, 1731, 1933
HapII	CCGG	3 cut(s)	110, 754, 1326
HgaI	GACGC	1 cut(s)	799
Hin1I	GRCGYC	1 cut(s)	810
HincII	GTYRAC	3 cut(s)	289, 1447, 1908
HindII	GTYRAC	3 cut(s)	289, 1447, 1908
HindIII	AAGCTT	2 cut(s)	990, 1778
HpaI	GTTAAC	1 cut(s)	289
HpaII	CCGG	3 cut(s)	110, 754, 1326
HphI	GGTGA	4 cut(s)	79, 716, 1129, 1547
Hpy166II	GTNNAC	6 cut(s)	254, 289, 1137, 1447, 1468, 1908
Hpy188I	TCNGA	8 cut(s)	168, 309, 793, 928, 1232, 1726, 1799, 1968
Hpy188III	TCNNGA	5 cut(s)	149, 263, 473, 1072, 1481
Hpy8I	GTNNAC	6 cut(s)	254, 289, 1137, 1447, 1468, 1908
Hpy99I	CGWCG	1 cut(s)	1952
HpyAV	CCTTC	5 cut(s)	140, 304, 1110, 1297, 1721
HpyCH4III	ACNGT	7 cut(s)	67, 566, 745, 820, 869, 1444, 1804
HpyCH4IV	ACGT	1 cut(s)	1080
HpyF10VI	GCNNNNNNNGC	7 cut(s)	246, 476, 686, 763, 1223, 1703, 1728
HpyF3I	CTNAG	7 cut(s)	134, 767, 1172, 1551, 1878, 1912, 1967
HpySE526I	ACGT	1 cut(s)	1080
Hsp92I	GRCGYC	1 cut(s)	810
KflI	GGGWCCC	1 cut(s)	433
KroI	GCCGGC	1 cut(s)	1325
KroNI	GCCGGC	1 cut(s)	1327
KspAI	GTTAAC	1 cut(s)	289
LmnI	GCTCC	3 cut(s)	314, 422, 514
Lsp1109I	GCAGC	4 cut(s)	100, 692, 1718, 1903
LweI	GCATC	5 cut(s)	349, 780, 846, 1096, 1684
MaeI	CTAG	6 cut(s)	270, 324, 1001, 1178, 1319, 1599
MaeII	ACGT	1 cut(s)	1080
MaeIII	GTNAC	4 cut(s)	15, 61, 745, 1349
MbiI	CCGCTC	1 cut(s)	240
MboII	GAAGA	6 cut(s)	164, 290, 462, 581, 1264, 1286
MflI	RGATCY	3 cut(s)	94, 532, 1279
MhlI	GDGCHC	4 cut(s)	256, 319, 899, 1542
MlyI	GAGTC	3 cut(s)	139, 1442, 1889
MmeI	TCCRAC	3 cut(s)	103, 821, 1527
Mph1103I	ATGCAT	3 cut(s)	518, 861, 1940
MroNI	GCCGGC	1 cut(s)	1325
MroXI	GAANNNNTTC	1 cut(s)	457
MseI	TTAA	9 cut(s)	288, 297, 729, 903, 909, 1050, 1062, 1497, 1646
MslI	CAYNNNNRTG	5 cut(s)	341, 378, 776, 1019, 1545
MspA1I	CMGCKG	2 cut(s)	1773, 1916
MspI	CCGG	3 cut(s)	110, 754, 1326
MspR9I	CCNGG	5 cut(s)	430, 609, 1199, 1593, 1719
Mva1269I	GAATGC	1 cut(s)	336
MvaI	CCWGG	5 cut(s)	430, 609, 1199, 1593, 1719
MwoI	GCNNNNNNNGC	7 cut(s)	246, 476, 686, 763, 1223, 1703, 1728
NaeI	GCCGGC	1 cut(s)	1327
NcoI	CCATGG	1 cut(s)	1140
NdeI	CATATG	2 cut(s)	655, 1516
NgoMIV	GCCGGC	1 cut(s)	1325
NlaIV	GGNNCC	6 cut(s)	434, 435, 898, 1026, 1086, 1590
NmuCI	GTSAC	1 cut(s)	745
NsiI	ATGCAT	3 cut(s)	518, 861, 1940
NspI	RCATGY	2 cut(s)	781, 863
PaeI	GCATGC	1 cut(s)	863
PaeR7I	CTCGAG	1 cut(s)	117
PctI	GAATGC	1 cut(s)	336
PdiI	GCCGGC	1 cut(s)	1327
PdmI	GAANNNNTTC	1 cut(s)	457
PfeI	GAWTC	7 cut(s)	493, 794, 1120, 1386, 1420, 1767, 1832
PflFI	GACNNNGTC	1 cut(s)	1016
PflMI	CCANNNNNTGG	1 cut(s)	659
PkrI	GCNGC	4 cut(s)	115, 682, 1708, 1893
PleI	GAGTC	3 cut(s)	139, 1442, 1888
PpsI	GAGTC	3 cut(s)	139, 1442, 1888
PpuMI	RGGWCCY	1 cut(s)	433
PshBI	ATTAAT	3 cut(s)	297, 729, 1050
PsiI	TTATAA	1 cut(s)	1038
Psp124BI	GAGCTC	1 cut(s)	319
Psp5II	RGGWCCY	1 cut(s)	433
Psp6I	CCWGG	5 cut(s)	428, 607, 1197, 1591, 1717
PspEI	GGTNACC	1 cut(s)	1349
PspGI	CCWGG	5 cut(s)	428, 607, 1197, 1591, 1717
PspN4I	GGNNCC	6 cut(s)	434, 435, 898, 1026, 1086, 1590
PspPI	GGNCC	3 cut(s)	433, 1024, 1595
PspPPI	RGGWCCY	1 cut(s)	433
PspXI	VCTCGAGB	1 cut(s)	117
PstNI	CAGNNNCTG	1 cut(s)	1724
PsuI	RGATCY	3 cut(s)	94, 532, 1279
PsyI	GACNNNGTC	1 cut(s)	1016
PvuII	CAGCTG	1 cut(s)	1916
RsaI	GTAC	2 cut(s)	89, 447
RsaNI	GTAC	2 cut(s)	88, 446
RseI	CAYNNNNRTG	5 cut(s)	341, 378, 776, 1019, 1545
SacI	GAGCTC	1 cut(s)	319
SaqAI	TTAA	9 cut(s)	288, 297, 729, 903, 909, 1050, 1062, 1497, 1646
SatI	GCNGC	4 cut(s)	114, 681, 1707, 1892
Sau96I	GGNCC	3 cut(s)	433, 1024, 1595
SchI	GAGTC	3 cut(s)	139, 1442, 1889
ScrFI	CCNGG	5 cut(s)	430, 609, 1199, 1593, 1719
SduI	GDGCHC	4 cut(s)	256, 319, 899, 1542
SfaNI	GCATC	5 cut(s)	349, 780, 846, 1096, 1684
SfcI	CTRYAG	3 cut(s)	562, 1468, 1803
Sfr274I	CTCGAG	1 cut(s)	117
SinI	GGWCC	2 cut(s)	433, 1024
SlaI	CTCGAG	1 cut(s)	117
SmiMI	CAYNNNNRTG	5 cut(s)	341, 378, 776, 1019, 1545
SmlI	CTYRAG	3 cut(s)	117, 890, 1450
SmoI	CTYRAG	3 cut(s)	117, 890, 1450
SphI	GCATGC	1 cut(s)	863
SsiI	CCGC	8 cut(s)	240, 404, 521, 1329, 1359, 1410, 1586, 1773
SspI	AATATT	1 cut(s)	399
SspMI	CTAG	6 cut(s)	270, 324, 1001, 1178, 1319, 1599
SstI	GAGCTC	1 cut(s)	319
StyD4I	CCNGG	5 cut(s)	428, 607, 1197, 1591, 1717
StyI	CCWWGG	2 cut(s)	1140, 1732
TaaI	ACNGT	7 cut(s)	67, 566, 745, 820, 869, 1444, 1804
TaiI	ACGT	1 cut(s)	1083
TaqI	TCGA	4 cut(s)	118, 547, 797, 1688
TatI	WGTACW	1 cut(s)	87
TfiI	GAWTC	7 cut(s)	493, 794, 1120, 1386, 1420, 1767, 1832
Tru1I	TTAA	9 cut(s)	288, 297, 729, 903, 909, 1050, 1062, 1497, 1646
Tru9I	TTAA	9 cut(s)	288, 297, 729, 903, 909, 1050, 1062, 1497, 1646
TscAI	CASTG	4 cut(s)	256, 571, 748, 1396
TseFI	GTSAC	1 cut(s)	745
TseI	GCWGC	4 cut(s)	113, 680, 1706, 1891
Tsp45I	GTSAC	1 cut(s)	745
TspDTI	ATGAA	6 cut(s)	224, 315, 356, 570, 1036, 1835
TspRI	CASTG	4 cut(s)	256, 571, 748, 1396
Tth111I	GACNNNGTC	1 cut(s)	1016
Van91I	CCANNNNNTGG	1 cut(s)	659
VneI	GTGCAC	1 cut(s)	252
VpaK11BI	GGWCC	2 cut(s)	433, 1024
VspI	ATTAAT	3 cut(s)	297, 729, 1050
XapI	RAATTY	3 cut(s)	302, 905, 1314
XceI	RCATGY	2 cut(s)	781, 863
XhoI	CTCGAG	1 cut(s)	117
XmiI	GTMKAC	1 cut(s)	1467
XmnI	GAANNNNTTC	1 cut(s)	457
XspI	CTAG	6 cut(s)	270, 324, 1001, 1178, 1319, 1599
Zsp2I	ATGCAT	3 cut(s)	518, 861, 1940

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.