Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0000924

Physical Location & Seq

Genomic Coordinates Reverse (-)

21291394 .. 21294362

Gene Structure 2969 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1177900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1893 bp

ATGCCAAAGGCAAGGTCGTTAGTCCTCTTGGTAGGACTTTTGTGCTTGTGCTGCTTGGGAGGCACGGCACAAGCAGAGGCGGAGTACATGGCATACAAGGACCCGAAACAGCCCGTAAATCATCGTATAAAGGACCTTATGGGTCGAATGAAGCTAGCCGAAAAGATTGGTCAGATGACGCAACTGGATCGTGCCAATGTCACAGCTGACATCATGAGGGACTTCTCATTAGGCAGTATACTGAGTGGCGGAGGGAGTGTGCCGCGGCTACAGGCCAGTCCACAGGACTGGATCAAAATGTTCAATGAGTTTCAAAATGGTTCACTTTCAAGCCGGCTTGGAATCCCGATGATTTATGGCATTGATGCGGTTCATGGCCACAATAATGTTTATAGGGCAACAATTTTCCCACATAATGTCGGACTTGGAGCTACTAGGCATGTGCAAAATATTGGTGCTGCAACTGCTCTTGAAGTTAGAGCTACTGGCATCAATTATGTATTTGCACCATGCATTGCGGTCTGCAGAGATCCGAGATGGGGTAGGTGTTACGAGAGTTATAGCGAAGATCCTGCAATAGTAGAACAAATGACTGATATCATACTCGGATTGCAAGGTGAACCTCCAGCTGGTTTAAGGAAGGGCGTTCCTTATGTTGGTGGAAAGGACAAGGTAGCCGCCTGTGCAAAGCACTTTGTTGGAGATGGCGGAACAGTCAAGGGCATCAATGAGAACAACACAGTGATTGATAGGCATGGACTGCTGAGCATTCATATGCCTGGTTACTACCATTCTATCATCAAGGGTGTCTCAACAATCATGGTGTCCTACTCGAGTGTGAACGGGAAGAAGATGCACGCCAATCACGAGTTTGTCACCAAATTTCTTAAGGGTACCCTTAAGTTCAGGGGTTTTGTCATCTCTGACTGGCAGGGTGTTGACAAAATTACCTATCCGCCCCATTCTGATTATCCAAACTCTGTTCTTGCTGGTATTCAAGCTGGCATTGACATGGTCATGGTTCCGTTCAATCACACCGAGTTCATTGATATCGTAACAACCCATGTCAACAACAAATTAATCCCCATGAGCCGTATTGATGATGCCGTCAAGAGAATTTTGCGAGTCAAGTTTGTGATGGGTTTGTTTGAGAACCCGTTGGCTGATCAGAGTTTTGTTGACAAGCTTGGAAGCCAGACTCATAGAGATCTGGCACGCGAAGCAGTCAGGAAGTCGCTTGTGCTGCTGAAGAATGGGGAAAATGCAGATTCACCAGTTCTACCGCTCTCTAAGAAGGCGAAAAAGATCCTAGTTGCTGGAACTCATGCCAACAACTTGGGCTATCAGTGTGGAGGTTGGAGTCTCACGTGGCAAGGACTTACTGGCAACAACAACACTGCTGGAACCACCATCCTAAGCGCCATCACAGCTGCAGTTGATCCCAGCACAGAAATTATGTTCAGCGAAAACCCTGATGCTGATTTTGTCAAGTCTAATAACTTTTCCTATGCCATTGTTGCCGTTGGGGAGCCCCCTTATGCTGAGACCAACGGGGATAGCTTGAATTTGACCATTGCAGAACCAGGTCCAAGCACCATCACCAATGTCTGTGGTGGAGTCAAATGCGTGGTTATCGTTGTCTCTGGCCGTCCTGTTGTGATTGAGCCCTCCGTTTCCTCGATTGATGCGCTTGTTGCTGCATGGCTTCCGGGAACTGAAGGCCATGGAGTGAGTGATGTTCTTTTTGGAGATTATGGATTCACAGGAAAGCTTCCTAGGACATGGTTCAAGACAGTAGATCAACTCCCTATGAATGTTGGTGATGCACATTACGATCCCCTCTTTCCCTTCGGTTTTGGACTTACAACCGATCCAGTTGATCAGTCATCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0000323 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005886 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0008422 GO:0009056 GO:0009057 GO:0009251 GO:0015926 GO:0016020 GO:0016052 GO:0016787 GO:0016798 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0071704 GO:0071944 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00460 ko00500 ko00940 ko01100 ko01110 map00460 map00500 map00940 map01100 map01110

Pfam Domains

Protein Families

Glyco_hydro_3 Glyco_hydro_3_C

Protein Analysis

631

Amino Acids

68.1

Weight (kDa)

7.0

Isoelectric Point (pI)

30.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_3	PF00933	52 - 376	3.5e-61	Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C	PF01915	413 - 622	3.6e-37	Glycosyl hydrolase family 3 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000924)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04885 AT5G04885 AT5G04885
fragaria_vesca	FvH4_1g28352 FvH4_1g28352 FvH4_1g28352 FvH4_4g26180 FvH4_4g26180 FvH4_7g05220 FvH4_7g05220
malus_domestica	MD02G1259600.v1.1 MD07G1060900.v1.1 MD11G1177800.v1.1 MD11G1177900.v1.1 MD14G1004200.v1.1
prunus_persica	Prupe.2G065700_v2.0.a1 Prupe.2G065700_v2.0.a1 Prupe.2G065700_v2.0.a1 Prupe.2G065700_v2.0.a1 Prupe.2G065700_v2.0.a1 Prupe.6G150000_v2.0.a1 Prupe.6G150200_v2.0.a1 Prupe.6G150400_v2.0.a1 Prupe.6G150500_v2.0.a1 Prupe.7G007200_v2.0.a1
pyrus_communis	pycom14g00250
rosa_chinensis	RchiOBHm_Chr1g0331601 RchiOBHm_Chr3g0496681 RchiOBHm_Chr3g0496721 RchiOBHm_Chr4g0433771
rosa_laevigata	RLG00000006732 RLG00000022510 RLG00000022511 RLG00000029727 RLG00000034619
rosa_multiflora	Rmu_sc0005044.1_g000017 Rmu_sc0006176.1_g000006
rosa_roxburghii	Rroxscaffold_4G00319030 Rroxscaffold_5G00375200 Rroxscaffold_6G00388790 Rroxscaffold_6G00388830 Rroxscaffold_6G00388850
rosa_rugosa	Rorug01G0095000 Rorug01G0095100 Rorug01G0095200 Rorug03G0185800 Rorug03G0186000 Rorug03G0285500 Rorug03G0285700 Rorug04G0271300 Rorug05G0248600 Rorug06G0034700
rosa_samantha	Rh1AG118800 Rh1CG113800 Rh3AG330600 Rh3BG366600 Rh3CG363300 Rh3CG363500 Rh4AG326000 Rh4CG349100 Rh5AG325000
rosa_wichuraiana	Rw1G009560 Rw3G028940 Rw3G028950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	893
AccB1I	GGYRCC	1 cut(s)	893
AccBSI	CCGCTC	1 cut(s)	1285
AccI	GTMKAC	1 cut(s)	238
AccII	CGCG	2 cut(s)	265, 1218
AclWI	GGATC	8 cut(s)	195, 299, 524, 563, 1300, 1433, 1829, 1865
AcoI	YGGCCR	2 cut(s)	376, 1645
AcsI	RAATTY	3 cut(s)	881, 1116, 1564
AcuI	CTGAAG	2 cut(s)	1268, 1737
AcvI	CACGTG	1 cut(s)	1368
AfaI	GTAC	2 cut(s)	86, 895
AfiI	CCNNNNNNNGG	3 cut(s)	539, 629, 656
AflII	CTTAAG	2 cut(s)	887, 899
AgsI	TTSAA	8 cut(s)	304, 314, 330, 473, 998, 1030, 1564, 1789
AjnI	CCWGG	2 cut(s)	778, 1582
Alw26I	GTCTC	4 cut(s)	814, 1367, 1538, 1645
AlwI	GGATC	8 cut(s)	195, 299, 524, 563, 1300, 1433, 1829, 1865
Ama87I	CYCGRG	1 cut(s)	832
AoxI	GGCC	4 cut(s)	273, 376, 1645, 1720
ApeKI	GCWGC	5 cut(s)	51, 458, 1243, 1430, 1697
ApoI	RAATTY	3 cut(s)	881, 1116, 1564
ArsI	GACNNNNNNTTYG	1 cut(s)	31
AseI	ATTAAT	1 cut(s)	1079
Asp718I	GGTACC	1 cut(s)	893
AspA2I	CCTAGG	1 cut(s)	1775
AspLEI	GCGC	2 cut(s)	1421, 1690
AspS9I	GGNCC	3 cut(s)	100, 133, 1586
AsuC2I	CCSGG	1 cut(s)	1710
AsuHPI	GGTGA	5 cut(s)	629, 868, 1263, 1591, 1832
AsuNHI	GCTAGC	1 cut(s)	154
AvaI	CYCGRG	1 cut(s)	832
AvaII	GGWCC	3 cut(s)	100, 133, 1586
AvrII	CCTAGG	1 cut(s)	1775
BalI	TGGCCA	1 cut(s)	378
BanI	GGYRCC	1 cut(s)	893
BanII	GRGCYC	2 cut(s)	1533, 1668
BauI	CACGAG	1 cut(s)	866
BbrPI	CACGTG	1 cut(s)	1368
BbvI	GCAGC	5 cut(s)	38, 445, 1230, 1417, 1684
BccI	CCATC	6 cut(s)	531, 698, 1132, 1418, 1430, 1604
BceAI	ACGGC	5 cut(s)	81, 1077, 1091, 1505, 1632
BcgI	CGANNNNNNTGC	4 cut(s)	170, 204, 554, 588
BciT130I	CCWGG	2 cut(s)	780, 1584
BclI	TGATCA	2 cut(s)	1165, 1879
BcnI	CCSGG	1 cut(s)	1710
BcoDI	GTCTC	4 cut(s)	814, 1367, 1538, 1645
BfaI	CTAG	4 cut(s)	155, 435, 1310, 1776
BfmI	CTRYAG	3 cut(s)	269, 523, 1431
BfoI	RGCGCY	1 cut(s)	1422
BfrI	CTTAAG	2 cut(s)	887, 899
BglII	AGATCT	1 cut(s)	1207
BisI	GCNGC	8 cut(s)	52, 263, 266, 459, 678, 1244, 1431, 1698
BlnI	CCTAGG	1 cut(s)	1775
BlpI	GCTNAGC	1 cut(s)	764
BlsI	GCNGC	8 cut(s)	53, 264, 267, 460, 679, 1245, 1432, 1699
Bme1390I	CCNGG	3 cut(s)	780, 1584, 1710
Bme18I	GGWCC	3 cut(s)	100, 133, 1586
BmeT110I	CYCGRG	1 cut(s)	832
BmgT120I	GGNCC	3 cut(s)	100, 133, 1586
BmiI	GGNNCC	5 cut(s)	102, 895, 1023, 1405, 1530
BmrFI	CCNGG	3 cut(s)	780, 1584, 1710
BmsI	GCATC	8 cut(s)	355, 498, 732, 843, 1093, 1465, 1675, 1813
BmtI	GCTAGC	1 cut(s)	158
BpmI	CTGGAG	1 cut(s)	609
Bpu10I	CCTNAGC	1 cut(s)	1415
Bpu1102I	GCTNAGC	1 cut(s)	764
BpuMI	CCSGG	1 cut(s)	1710
BsaAI	YACGTR	1 cut(s)	1368
BsaI	GGTCTC	1 cut(s)	1538
BsaJI	CCNNGG	3 cut(s)	263, 1723, 1775
BsaXI	ACNNNNNCTCC	2 cut(s)	243, 273
Bsc4I	CCNNNNNNNGG	3 cut(s)	539, 629, 656
Bse118I	RCCGGY	1 cut(s)	333
Bse1I	ACTGG	8 cut(s)	189, 276, 293, 490, 932, 1274, 1387, 1874
Bse3DI	GCAATG	2 cut(s)	513, 1572
BseBI	CCWGG	2 cut(s)	780, 1584
BseDI	CCNNGG	3 cut(s)	263, 1723, 1775
BseGI	GGATG	1 cut(s)	1410
BseLI	CCNNNNNNNGG	3 cut(s)	539, 629, 656
BseMI	GCAATG	2 cut(s)	513, 1572
BseMII	CTCAG	3 cut(s)	233, 755, 1533
BseNI	ACTGG	8 cut(s)	189, 276, 293, 490, 932, 1274, 1387, 1874
BseXI	GCAGC	5 cut(s)	38, 445, 1230, 1417, 1684
BseYI	CCCAGC	1 cut(s)	1442
Bsh1236I	CGCG	2 cut(s)	265, 1218
BshFI	GGCC	4 cut(s)	275, 378, 1647, 1722
BshNI	GGYRCC	1 cut(s)	893
BsiHKCI	CYCGRG	1 cut(s)	832
BsiSI	CCGG	2 cut(s)	334, 1709
BslFI	GGGAC	1 cut(s)	233
BslI	CCNNNNNNNGG	3 cut(s)	539, 629, 656
BsmAI	GTCTC	4 cut(s)	814, 1367, 1538, 1645
BsmFI	GGGAC	1 cut(s)	233
BsmI	GAATGC	1 cut(s)	768
BsnI	GGCC	4 cut(s)	275, 378, 1647, 1722
Bso31I	GGTCTC	1 cut(s)	1538
BsoBI	CYCGRG	1 cut(s)	832
Bsp1286I	GDGCHC	2 cut(s)	1533, 1668
Bsp1720I	GCTNAGC	1 cut(s)	764
Bsp19I	CCATGG	1 cut(s)	1723
BspANI	GGCC	4 cut(s)	275, 378, 1647, 1722
BspCNI	CTCAG	3 cut(s)	234, 756, 1534
BspFNI	CGCG	2 cut(s)	265, 1218
BspHI	TCATGA	1 cut(s)	213
BspLI	GGNNCC	5 cut(s)	102, 895, 1023, 1405, 1530
BspMAI	CTGCAG	2 cut(s)	527, 1435
BspOI	GCTAGC	1 cut(s)	158
BspPI	GGATC	8 cut(s)	195, 299, 524, 563, 1300, 1433, 1829, 1865
BspT107I	GGYRCC	1 cut(s)	893
BspTI	CTTAAG	2 cut(s)	887, 899
BspTNI	GGTCTC	1 cut(s)	1538
BsrBI	CCGCTC	1 cut(s)	1285
BsrDI	GCAATG	2 cut(s)	513, 1572
BsrFI	RCCGGY	1 cut(s)	333
BsrI	ACTGG	8 cut(s)	189, 276, 293, 490, 932, 1274, 1387, 1874
BssAI	RCCGGY	1 cut(s)	333
BssECI	CCNNGG	3 cut(s)	263, 1723, 1775
BssNAI	GTATAC	1 cut(s)	239
BssSI	CACGAG	1 cut(s)	866
BssT1I	CCWWGG	2 cut(s)	1723, 1775
Bst1107I	GTATAC	1 cut(s)	239
Bst2BI	CACGAG	1 cut(s)	866
Bst2UI	CCWGG	2 cut(s)	780, 1584
Bst4CI	ACNGT	3 cut(s)	715, 742, 1795
BstAFI	CTTAAG	2 cut(s)	887, 899
BstAPI	GCANNNNNTGC	1 cut(s)	760
BstBAI	YACGTR	1 cut(s)	1368
BstC8I	GCNNGC	5 cut(s)	156, 335, 858, 1003, 1216
BstDEI	CTNAG	6 cut(s)	242, 764, 1290, 1415, 1542, 1890
BstDSI	CCRYGG	2 cut(s)	263, 1723
BstF5I	GGATG	1 cut(s)	1410
BstFNI	CGCG	2 cut(s)	265, 1218
BstH2I	RGCGCY	1 cut(s)	1422
BstHHI	GCGC	2 cut(s)	1421, 1690
BstMAI	GTCTC	4 cut(s)	814, 1367, 1538, 1645
BstNI	CCWGG	2 cut(s)	780, 1584
BstNSI	RCATGY	1 cut(s)	443
BstSCI	CCNGG	3 cut(s)	778, 1582, 1708
BstSFI	CTRYAG	3 cut(s)	269, 523, 1431
BstUI	CGCG	2 cut(s)	265, 1218
BstV1I	GCAGC	5 cut(s)	38, 445, 1230, 1417, 1684
BstX2I	RGATCY	4 cut(s)	529, 568, 1207, 1305
BstXI	CCANNNNNNTGG	2 cut(s)	288, 1336
BstYI	RGATCY	4 cut(s)	529, 568, 1207, 1305
BstZ17I	GTATAC	1 cut(s)	239
BsuRI	GGCC	4 cut(s)	275, 378, 1647, 1722
BtgI	CCRYGG	2 cut(s)	263, 1723
BtsCI	GGATG	1 cut(s)	1410
BtsI	GCAGTG	1 cut(s)	1395
BtsIMutI	CAGTG	3 cut(s)	747, 1352, 1395
Cac8I	GCNNGC	5 cut(s)	156, 335, 858, 1003, 1216
CciI	TCATGA	1 cut(s)	213
CfoI	GCGC	2 cut(s)	1421, 1690
Cfr10I	RCCGGY	1 cut(s)	333
Cfr13I	GGNCC	3 cut(s)	100, 133, 1586
Cfr42I	CCGCGG	1 cut(s)	266
CseI	GACGC	1 cut(s)	187
CsiI	ACCWGGT	1 cut(s)	1582
Csp6I	GTAC	2 cut(s)	85, 894
CspCI	CAANNNNNGTGG	2 cut(s)	1591, 1626
CviQI	GTAC	2 cut(s)	85, 894
DdeI	CTNAG	6 cut(s)	242, 764, 1290, 1415, 1542, 1890
EaeI	YGGCCR	2 cut(s)	376, 1645
EciI	GGCGGA	4 cut(s)	95, 264, 723, 945
Eco130I	CCWWGG	2 cut(s)	1723, 1775
Eco24I	GRGCYC	2 cut(s)	1533, 1668
Eco31I	GGTCTC	1 cut(s)	1538
Eco32I	GATATC	2 cut(s)	598, 1051
Eco47I	GGWCC	3 cut(s)	100, 133, 1586
Eco57I	CTGAAG	2 cut(s)	1268, 1737
Eco72I	CACGTG	1 cut(s)	1368
Eco88I	CYCGRG	1 cut(s)	832
EcoO109I	RGGNCCY	2 cut(s)	100, 133
EcoRII	CCWGG	2 cut(s)	778, 1582
EcoRV	GATATC	2 cut(s)	598, 1051
EcoT14I	CCWWGG	2 cut(s)	1723, 1775
EcoT22I	ATGCAT	1 cut(s)	515
EcoT38I	GRGCYC	2 cut(s)	1533, 1668
ErhI	CCWWGG	2 cut(s)	1723, 1775
FaqI	GGGAC	1 cut(s)	233
FauNDI	CATATG	1 cut(s)	774
FbaI	TGATCA	2 cut(s)	1165, 1879
FblI	GTMKAC	1 cut(s)	238
Fnu4HI	GCNGC	8 cut(s)	52, 263, 266, 459, 678, 1244, 1431, 1698
FokI	GGATG	1 cut(s)	1397
FriOI	GRGCYC	2 cut(s)	1533, 1668
Fsp4HI	GCNGC	8 cut(s)	52, 263, 266, 459, 678, 1244, 1431, 1698
FspBI	CTAG	4 cut(s)	155, 435, 1310, 1776
GlaI	GCGC	2 cut(s)	1420, 1689
GluI	GCNGC	8 cut(s)	52, 263, 266, 459, 678, 1244, 1431, 1698
GsaI	CCCAGC	1 cut(s)	1446
GsuI	CTGGAG	1 cut(s)	609
HaeII	RGCGCY	1 cut(s)	1422
HaeIII	GGCC	4 cut(s)	275, 378, 1647, 1722
HapII	CCGG	2 cut(s)	334, 1709
HgaI	GACGC	1 cut(s)	187
HhaI	GCGC	2 cut(s)	1421, 1690
Hin6I	GCGC	2 cut(s)	1419, 1688
HinP1I	GCGC	2 cut(s)	1419, 1688
HincII	GTYRAC	3 cut(s)	940, 1069, 1180
HindII	GTYRAC	3 cut(s)	940, 1069, 1180
HindIII	AAGCTT	2 cut(s)	1184, 1769
HinfI	GANTC	7 cut(s)	342, 1125, 1198, 1268, 1360, 1617, 1758
HpaII	CCGG	2 cut(s)	334, 1709
HphI	GGTGA	5 cut(s)	629, 868, 1263, 1591, 1832
Hpy166II	GTNNAC	8 cut(s)	239, 281, 323, 620, 841, 940, 1069, 1180
Hpy188I	TCNGA	7 cut(s)	174, 422, 534, 608, 925, 967, 1170
Hpy188III	TCNNGA	7 cut(s)	214, 346, 470, 866, 1111, 1228, 1789
Hpy8I	GTNNAC	8 cut(s)	239, 281, 323, 620, 841, 940, 1069, 1180
HpyAV	CCTTC	4 cut(s)	634, 1288, 1712, 1858
HpyCH4III	ACNGT	3 cut(s)	715, 742, 1795
HpyCH4IV	ACGT	1 cut(s)	1367
HpyF3I	CTNAG	6 cut(s)	242, 764, 1290, 1415, 1542, 1890
HpySE526I	ACGT	1 cut(s)	1367
HspAI	GCGC	2 cut(s)	1419, 1688
KpnI	GGTACC	1 cut(s)	897
KroI	GCCGGC	1 cut(s)	333
KroNI	GCCGGC	1 cut(s)	335
Ksp22I	TGATCA	2 cut(s)	1165, 1879
KspI	CCGCGG	1 cut(s)	266
LmnI	GCTCC	2 cut(s)	428, 1528
Lsp1109I	GCAGC	5 cut(s)	38, 445, 1230, 1417, 1684
LweI	GCATC	8 cut(s)	355, 498, 732, 843, 1093, 1465, 1675, 1813
MabI	ACCWGGT	1 cut(s)	1582
MaeI	CTAG	4 cut(s)	155, 435, 1310, 1776
MaeII	ACGT	1 cut(s)	1367
MaeIII	GTNAC	5 cut(s)	199, 548, 782, 874, 1054
MbiI	CCGCTC	1 cut(s)	1285
MboII	GAAGA	4 cut(s)	578, 859, 862, 1261
MflI	RGATCY	4 cut(s)	529, 568, 1207, 1305
MhlI	GDGCHC	2 cut(s)	1533, 1668
MlsI	TGGCCA	1 cut(s)	378
MluCI	AATT	8 cut(s)	402, 493, 881, 945, 1076, 1116, 1452, 1564
MluNI	TGGCCA	1 cut(s)	378
MlyI	GAGTC	4 cut(s)	1134, 1192, 1369, 1626
MmeI	TCCRAC	3 cut(s)	400, 679, 1337
Mox20I	TGGCCA	1 cut(s)	378
Mph1103I	ATGCAT	1 cut(s)	515
MroNI	GCCGGC	1 cut(s)	333
MscI	TGGCCA	1 cut(s)	378
MseI	TTAA	4 cut(s)	635, 888, 900, 1079
MslI	CAYNNNNRTG	4 cut(s)	384, 773, 1010, 1016
Msp20I	TGGCCA	1 cut(s)	378
MspA1I	CMGCKG	4 cut(s)	206, 265, 629, 1430
MspCI	CTTAAG	2 cut(s)	887, 899
MspI	CCGG	2 cut(s)	334, 1709
MspR9I	CCNGG	3 cut(s)	780, 1584, 1710
Mva1269I	GAATGC	1 cut(s)	768
MvaI	CCWGG	2 cut(s)	780, 1584
MvnI	CGCG	2 cut(s)	265, 1218
NaeI	GCCGGC	1 cut(s)	335
NciI	CCSGG	1 cut(s)	1710
NcoI	CCATGG	1 cut(s)	1723
NdeI	CATATG	1 cut(s)	774
NgoMIV	GCCGGC	1 cut(s)	333
NheI	GCTAGC	1 cut(s)	154
NlaIV	GGNNCC	5 cut(s)	102, 895, 1023, 1405, 1530
NmuCI	GTSAC	2 cut(s)	199, 874
NsiI	ATGCAT	1 cut(s)	515
NspI	RCATGY	1 cut(s)	443
PaeR7I	CTCGAG	1 cut(s)	832
PagI	TCATGA	1 cut(s)	213
PcsI	WCGNNNNNNNCGW	1 cut(s)	864
PctI	GAATGC	1 cut(s)	768
PdiI	GCCGGC	1 cut(s)	335
PfeI	GAWTC	3 cut(s)	342, 1268, 1758
PflFI	GACNNNGTC	1 cut(s)	1013
PfoI	TCCNGGA	1 cut(s)	1708
PkrI	GCNGC	8 cut(s)	53, 264, 267, 460, 679, 1245, 1432, 1699
PleI	GAGTC	4 cut(s)	1133, 1192, 1368, 1625
PmaCI	CACGTG	1 cut(s)	1368
PmlI	CACGTG	1 cut(s)	1368
PpsI	GAGTC	4 cut(s)	1133, 1192, 1368, 1625
Ppu21I	YACGTR	1 cut(s)	1368
PpuMI	RGGWCCY	2 cut(s)	100, 133
PshBI	ATTAAT	1 cut(s)	1079
Psp5II	RGGWCCY	2 cut(s)	100, 133
Psp6I	CCWGG	2 cut(s)	778, 1582
PspCI	CACGTG	1 cut(s)	1368
PspFI	CCCAGC	1 cut(s)	1442
PspGI	CCWGG	2 cut(s)	778, 1582
PspN4I	GGNNCC	5 cut(s)	102, 895, 1023, 1405, 1530
PspPI	GGNCC	3 cut(s)	100, 133, 1586
PspPPI	RGGWCCY	2 cut(s)	100, 133
PspXI	VCTCGAGB	1 cut(s)	832
PstI	CTGCAG	2 cut(s)	527, 1435
PsuI	RGATCY	4 cut(s)	529, 568, 1207, 1305
PsyI	GACNNNGTC	1 cut(s)	1013
PvuII	CAGCTG	3 cut(s)	206, 629, 1430
RsaI	GTAC	2 cut(s)	86, 895
RsaNI	GTAC	2 cut(s)	85, 894
RseI	CAYNNNNRTG	4 cut(s)	384, 773, 1010, 1016
SacII	CCGCGG	1 cut(s)	266
SaqAI	TTAA	4 cut(s)	635, 888, 900, 1079
SatI	GCNGC	8 cut(s)	52, 263, 266, 459, 678, 1244, 1431, 1698
Sau96I	GGNCC	3 cut(s)	100, 133, 1586
SchI	GAGTC	4 cut(s)	1134, 1192, 1369, 1626
ScrFI	CCNGG	3 cut(s)	780, 1584, 1710
SduI	GDGCHC	2 cut(s)	1533, 1668
SexAI	ACCWGGT	1 cut(s)	1582
SfaNI	GCATC	8 cut(s)	355, 498, 732, 843, 1093, 1465, 1675, 1813
SfcI	CTRYAG	3 cut(s)	269, 523, 1431
Sfr274I	CTCGAG	1 cut(s)	832
Sfr303I	CCGCGG	1 cut(s)	266
SgrBI	CCGCGG	1 cut(s)	266
SinI	GGWCC	3 cut(s)	100, 133, 1586
SlaI	CTCGAG	1 cut(s)	832
SmiMI	CAYNNNNRTG	4 cut(s)	384, 773, 1010, 1016
SmlI	CTYRAG	3 cut(s)	832, 887, 899
SmoI	CTYRAG	3 cut(s)	832, 887, 899
Sse9I	AATT	8 cut(s)	402, 493, 881, 945, 1076, 1116, 1452, 1564
SspI	AATATT	1 cut(s)	451
SspMI	CTAG	4 cut(s)	155, 435, 1310, 1776
StyD4I	CCNGG	3 cut(s)	778, 1582, 1708
StyI	CCWWGG	2 cut(s)	1723, 1775
TaaI	ACNGT	3 cut(s)	715, 742, 1795
TaiI	ACGT	1 cut(s)	1370
TaqI	TCGA	3 cut(s)	145, 833, 1679
TasI	AATT	8 cut(s)	402, 493, 881, 945, 1076, 1116, 1452, 1564
TatI	WGTACW	1 cut(s)	84
TauI	GCSGC	3 cut(s)	265, 268, 680
TfiI	GAWTC	3 cut(s)	342, 1268, 1758
Tru1I	TTAA	4 cut(s)	635, 888, 900, 1079
Tru9I	TTAA	4 cut(s)	635, 888, 900, 1079
TscAI	CASTG	3 cut(s)	747, 1352, 1402
TseFI	GTSAC	2 cut(s)	199, 874
TseI	GCWGC	5 cut(s)	51, 458, 1243, 1430, 1697
Tsp45I	GTSAC	2 cut(s)	199, 874
TspDTI	ATGAA	5 cut(s)	164, 362, 761, 1033, 1826
TspGWI	ACGGA	2 cut(s)	1014, 1660
TspRI	CASTG	3 cut(s)	747, 1352, 1402
Tth111I	GACNNNGTC	1 cut(s)	1013
Vha464I	CTTAAG	2 cut(s)	887, 899
VpaK11BI	GGWCC	3 cut(s)	100, 133, 1586
VspI	ATTAAT	1 cut(s)	1079
XapI	RAATTY	3 cut(s)	881, 1116, 1564
XceI	RCATGY	1 cut(s)	443
XhoI	CTCGAG	1 cut(s)	832
XmaJI	CCTAGG	1 cut(s)	1775
XmiI	GTMKAC	1 cut(s)	238
XspI	CTAG	4 cut(s)	155, 435, 1310, 1776
Zsp2I	ATGCAT	1 cut(s)	515

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.