FvH4_1g29020

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0003619
Physical Location & Seq
Reverse (-)
21444375 .. 21446539
2165 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g29020.t1

Sequence Viewer

Length: 606 bp
ATGTTCCTCTGCTCTGCATTTTTTTCGTTTGTTTTCTGTGTTGCGTTTTGGAGGCTCGATGGAGAGCTGTGCTTTGATTTAAAGTTACCCTTGTCAACGATGCACTGCCCCTATTGGCTGTCAGTAAGTAACACTGGAGGAGGAAAGCACTGCAAAAAGGTGACCAAAGTGGTGGTTCCGAGTGGTGAAATACGAGAGTTGAATGAACCAGTAAAAGCCGCGGAGGTGATGCTCGAGACCCCGAACTTCTTCCTAGTAAACACCAAGTGTCTCCATATGGGCAGCAGATTTTCTGCTCTCAATGCCGACCATGACTTGGAGATGGGCAGCGTTTACGTCATGTTTCCCATGAAGAGGCTCAACTCCACCGTCACATCATCTGACATGTCCGCCTTGTTCCTCACCGCCGTTGCCACTCCCAAACCCAAACGGCAGCTTTCCGCTCATCGTAACATGAATTGGCAAGAGGCAACAGACAAGCTAAATCTGGATGATATCCAAGAGTTCTCGGCCCCGGAATTCATGCATCGATTATCCATGACTAGGTCCAAGAAGCCATTGTTGGAGACCATAACAGAAGAGACTGTTAGTGTTTGTTCAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

202

Amino Acids

22.74

Weight (kDa)

7.62

Isoelectric Point (pI)

34.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 49 - 193 4.2e-32 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 316
AccBSI CCGCTC 1 cut(s) 443
AccII CGCG 1 cut(s) 221
AciI CCGC 5 cut(s) 219, 221, 390, 405, 441
AcsI RAATTY 1 cut(s) 518
AdeI CACNNNGTG 1 cut(s) 267
AfiI CCNNNNNNNGG 3 cut(s) 316, 354, 543
AflIII ACRYGT 1 cut(s) 384
AgsI TTSAA 2 cut(s) 202, 600
AjuI GAANNNNNNNTTGG 2 cut(s) 545, 577
AluBI AGCT 3 cut(s) 67, 436, 481
AluI AGCT 3 cut(s) 67, 436, 481
Alw26I GTCTC 4 cut(s) 230, 275, 560, 575
Ama87I CYCGRG 1 cut(s) 233
AoxI GGCC 1 cut(s) 510
ApeKI GCWGC 3 cut(s) 282, 327, 433
ApoI RAATTY 1 cut(s) 518
Asp700I GAANNNNTTC 1 cut(s) 248
AspS9I GGNCC 2 cut(s) 511, 546
AsuC2I CCSGG 1 cut(s) 515
AsuHPI GGTGA 4 cut(s) 172, 197, 238, 394
AvaI CYCGRG 1 cut(s) 233
AvaII GGWCC 1 cut(s) 546
BbvI GCAGC 3 cut(s) 294, 339, 445
BccI CCATC 2 cut(s) 53, 316
BceAI ACGGC 2 cut(s) 392, 446
BcgI CGANNNNNNTGC 2 cut(s) 6, 40
BcnI CCSGG 1 cut(s) 515
BcoDI GTCTC 4 cut(s) 230, 275, 560, 575
BfaI CTAG 2 cut(s) 254, 543
BisI GCNGC 4 cut(s) 219, 283, 328, 434
BlsI GCNGC 4 cut(s) 220, 284, 329, 435
Bme1390I CCNGG 1 cut(s) 515
Bme18I GGWCC 1 cut(s) 546
BmeT110I CYCGRG 1 cut(s) 233
BmgT120I GGNCC 2 cut(s) 511, 546
BmiI GGNNCC 2 cut(s) 177, 513
BmrFI CCNGG 1 cut(s) 515
BmsI GCATC 3 cut(s) 90, 219, 535
BpmI CTGGAG 1 cut(s) 156
BpuMI CCSGG 1 cut(s) 515
Bsa29I ATCGAT 1 cut(s) 529
BsaI GGTCTC 2 cut(s) 230, 560
BsaJI CCNNGG 2 cut(s) 219, 513
Bsc4I CCNNNNNNNGG 3 cut(s) 316, 354, 543
Bse1I ACTGG 2 cut(s) 139, 209
BseCI ATCGAT 1 cut(s) 529
BseDI CCNNGG 2 cut(s) 219, 513
BseGI GGATG 1 cut(s) 496
BseLI CCNNNNNNNGG 3 cut(s) 316, 354, 543
BseNI ACTGG 2 cut(s) 139, 209
BseRI GAGGAG 1 cut(s) 153
BseXI GCAGC 3 cut(s) 294, 339, 445
Bsh1236I CGCG 1 cut(s) 221
BshFI GGCC 1 cut(s) 512
BshVI ATCGAT 1 cut(s) 529
BsiHKCI CYCGRG 1 cut(s) 233
BsiSI CCGG 1 cut(s) 515
BslI CCNNNNNNNGG 3 cut(s) 316, 354, 543
BsmAI GTCTC 4 cut(s) 230, 275, 560, 575
BsnI GGCC 1 cut(s) 512
Bso31I GGTCTC 2 cut(s) 230, 560
BsoBI CYCGRG 1 cut(s) 233
BspACI CCGC 5 cut(s) 219, 221, 390, 405, 441
BspANI GGCC 1 cut(s) 512
BspDI ATCGAT 1 cut(s) 529
BspFNI CGCG 1 cut(s) 221
BspLI GGNNCC 2 cut(s) 177, 513
BspTNI GGTCTC 2 cut(s) 230, 560
BsrBI CCGCTC 1 cut(s) 443
BsrI ACTGG 2 cut(s) 139, 209
BssECI CCNNGG 2 cut(s) 219, 513
Bst4CI ACNGT 2 cut(s) 370, 586
Bst6I CTCTTC 2 cut(s) 347, 573
BstDSI CCRYGG 1 cut(s) 219
BstEII GGTNACC 1 cut(s) 160
BstF5I GGATG 1 cut(s) 496
BstFNI CGCG 1 cut(s) 221
BstMAI GTCTC 4 cut(s) 230, 275, 560, 575
BstMWI GCNNNNNNNGC 1 cut(s) 302
BstNSI RCATGY 1 cut(s) 388
BstPI GGTNACC 1 cut(s) 160
BstSCI CCNGG 1 cut(s) 513
BstUI CGCG 1 cut(s) 221
BstV1I GCAGC 3 cut(s) 294, 339, 445
BstXI CCANNNNNNTGG 1 cut(s) 172
Bsu15I ATCGAT 1 cut(s) 529
BsuRI GGCC 1 cut(s) 512
BsuTUI ATCGAT 1 cut(s) 529
BtgI CCRYGG 1 cut(s) 219
BtsCI GGATG 1 cut(s) 496
BtsI GCAGTG 2 cut(s) 103, 148
BtsIMutI CAGTG 3 cut(s) 103, 132, 148
Cfr13I GGNCC 2 cut(s) 511, 546
Cfr42I CCGCGG 1 cut(s) 222
ClaI ATCGAT 1 cut(s) 529
CviAII CATG 7 cut(s) 311, 340, 349, 385, 454, 523, 538
CviJI RGCY 9 cut(s) 55, 67, 118, 218, 358, 436, 481, 512, 556
CviKI_1 RGCY 9 cut(s) 55, 67, 118, 218, 358, 436, 481, 512, 556
DraI TTTAAA 1 cut(s) 81
DraIII CACNNNGTG 1 cut(s) 267
Eam1104I CTCTTC 2 cut(s) 347, 573
EarI CTCTTC 2 cut(s) 347, 573
EciI GGCGGA 1 cut(s) 379
Eco31I GGTCTC 2 cut(s) 230, 560
Eco32I GATATC 1 cut(s) 496
Eco47I GGWCC 1 cut(s) 546
Eco88I CYCGRG 1 cut(s) 233
Eco91I GGTNACC 1 cut(s) 160
EcoO65I GGTNACC 1 cut(s) 160
EcoRI GAATTC 1 cut(s) 518
EcoRV GATATC 1 cut(s) 496
EcoT22I ATGCAT 1 cut(s) 528
FaeI CATG 7 cut(s) 314, 343, 352, 388, 457, 526, 541
FalI AAGNNNNNCTT 2 cut(s) 74, 106
FatI CATG 7 cut(s) 310, 339, 348, 384, 453, 522, 537
FauNDI CATATG 1 cut(s) 276
Fnu4HI GCNGC 4 cut(s) 219, 283, 328, 434
FokI GGATG 1 cut(s) 503
Fsp4HI GCNGC 4 cut(s) 219, 283, 328, 434
FspBI CTAG 2 cut(s) 254, 543
GluI GCNGC 4 cut(s) 219, 283, 328, 434
GsuI CTGGAG 1 cut(s) 156
HaeIII GGCC 1 cut(s) 512
HapII CCGG 1 cut(s) 515
Hin1II CATG 7 cut(s) 314, 343, 352, 388, 457, 526, 541
HincII GTYRAC 1 cut(s) 96
HindII GTYRAC 1 cut(s) 96
HpaII CCGG 1 cut(s) 515
HphI GGTGA 4 cut(s) 172, 197, 238, 394
Hpy166II GTNNAC 3 cut(s) 96, 259, 334
Hpy188I TCNGA 2 cut(s) 180, 382
Hpy188III TCNNGA 2 cut(s) 235, 488
Hpy8I GTNNAC 3 cut(s) 96, 259, 334
HpyCH4III ACNGT 2 cut(s) 370, 586
HpyCH4IV ACGT 1 cut(s) 336
HpyCH4V TGCA 4 cut(s) 17, 103, 153, 526
HpyF10VI GCNNNNNNNGC 1 cut(s) 302
HpySE526I ACGT 1 cut(s) 336
Hsp92II CATG 7 cut(s) 314, 343, 352, 388, 457, 526, 541
KspI CCGCGG 1 cut(s) 222
LpnPI CCDG 4 cut(s) 120, 222, 473, 528
Lsp1109I GCAGC 3 cut(s) 294, 339, 445
LweI GCATC 3 cut(s) 90, 219, 535
MaeI CTAG 2 cut(s) 254, 543
MaeII ACGT 1 cut(s) 336
MaeIII GTNAC 5 cut(s) 84, 128, 160, 370, 449
MbiI CCGCTC 1 cut(s) 443
MboII GAAGA 3 cut(s) 241, 364, 590
MluCI AATT 2 cut(s) 457, 518
MmeI TCCRAC 1 cut(s) 543
MnlI CCTC 8 cut(s) 17, 45, 131, 134, 217, 348, 410, 460
Mph1103I ATGCAT 1 cut(s) 528
MroXI GAANNNNTTC 1 cut(s) 248
MseI TTAA 1 cut(s) 80
MspA1I CMGCKG 1 cut(s) 221
MspI CCGG 1 cut(s) 515
MspR9I CCNGG 1 cut(s) 515
MvnI CGCG 1 cut(s) 221
MwoI GCNNNNNNNGC 1 cut(s) 302
NciI CCSGG 1 cut(s) 515
NdeI CATATG 1 cut(s) 276
NlaIII CATG 7 cut(s) 314, 343, 352, 388, 457, 526, 541
NlaIV GGNNCC 2 cut(s) 177, 513
NmeAIII GCCGAG 1 cut(s) 488
NmuCI GTSAC 2 cut(s) 160, 370
NsiI ATGCAT 1 cut(s) 528
NspI RCATGY 1 cut(s) 388
PaeR7I CTCGAG 1 cut(s) 233
PciI ACATGT 1 cut(s) 384
PdmI GAANNNNTTC 1 cut(s) 248
PflFI GACNNNGTC 1 cut(s) 544
PflMI CCANNNNNTGG 1 cut(s) 316
PkrI GCNGC 4 cut(s) 220, 284, 329, 435
PscI ACATGT 1 cut(s) 384
PspEI GGTNACC 1 cut(s) 160
PspN4I GGNNCC 2 cut(s) 177, 513
PspPI GGNCC 2 cut(s) 511, 546
PsyI GACNNNGTC 1 cut(s) 544
SacII CCGCGG 1 cut(s) 222
SaqAI TTAA 1 cut(s) 80
SatI GCNGC 4 cut(s) 219, 283, 328, 434
Sau96I GGNCC 2 cut(s) 511, 546
ScrFI CCNGG 1 cut(s) 515
SetI ASST 8 cut(s) 69, 162, 228, 339, 438, 483, 548, 605
SfaNI GCATC 3 cut(s) 90, 219, 535
Sfr274I CTCGAG 1 cut(s) 233
Sfr303I CCGCGG 1 cut(s) 222
SgrBI CCGCGG 1 cut(s) 222
SinI GGWCC 1 cut(s) 546
SlaI CTCGAG 1 cut(s) 233
SmlI CTYRAG 1 cut(s) 233
SmoI CTYRAG 1 cut(s) 233
Sse9I AATT 2 cut(s) 457, 518
SsiI CCGC 5 cut(s) 219, 221, 390, 405, 441
SspMI CTAG 2 cut(s) 254, 543
StyD4I CCNGG 1 cut(s) 513
TaaI ACNGT 2 cut(s) 370, 586
TaiI ACGT 1 cut(s) 339
TaqI TCGA 3 cut(s) 57, 234, 529
TasI AATT 2 cut(s) 457, 518
TauI GCSGC 1 cut(s) 221
Tru1I TTAA 1 cut(s) 80
Tru9I TTAA 1 cut(s) 80
TscAI CASTG 3 cut(s) 110, 139, 155
TseFI GTSAC 2 cut(s) 160, 370
TseI GCWGC 3 cut(s) 282, 327, 433
Tsp45I GTSAC 2 cut(s) 160, 370
TspDTI ATGAA 4 cut(s) 219, 365, 470, 511
TspRI CASTG 3 cut(s) 110, 139, 155
Tth111I GACNNNGTC 1 cut(s) 544
Van91I CCANNNNNTGG 1 cut(s) 316
VpaK11BI GGWCC 1 cut(s) 546
XapI RAATTY 1 cut(s) 518
XceI RCATGY 1 cut(s) 388
XhoI CTCGAG 1 cut(s) 233
XmnI GAANNNNTTC 1 cut(s) 248
XspI CTAG 2 cut(s) 254, 543
Zsp2I ATGCAT 1 cut(s) 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.