RchiOBHm_Chr3g0493681

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0003619
Physical Location & Seq
Forward (+)
40978768 .. 40979350
583 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45642

Sequence Viewer

Length: 567 bp
ATGGGCAACTACATTTCTTGCAGTCTGTCAAGTAATACTGGAGGAGGAAAGCACTGCAAAGTGACCAAAGTAGTGGTTCCAAGCGGTGAAGTTCGAGAGATGAATGAACCGGTAAAAGCCGCGGAGGTGATGCTCGACACCCCAAACTACTTCCTGGTAAACTCCAAGTATCTCCATATGGGCAGGAGGTTTTCTGCTCTTACTGCAGACCAAGACTTGGAGATGGGCAGCGTTTACGTCATGTTTCCCATGAAGAGGCTCAACTCTACGGTCACATCTTCTGACATGGCCGCCTTGTTCCTCACCGCCGTTGCCACTCCCAAGGCCATGCACAAGCCCAAACGGCAGCTTTCCGGTGGCAAGGTCAGGGTGCGGCCGGCTGATCATGACGATCACGATCAAACCACCATTAATAAGATGTATTCAGACAAGGGGCCAAAGCTAAATTTGGATGATATCGAAGAGTTCTCGGCCCCAGAGTTCATGCATCGATTATCCATGAGTAGGTCCAAGAAGCCATTGTTGGAGACCATAACAGAAGAGGCTGTTAGTGTTTGTTCAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

188

Amino Acids

20.89

Weight (kDa)

8.72

Isoelectric Point (pI)

49.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 180 8.5e-38 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 217
AccII CGCG 1 cut(s) 122
AciI CCGC 6 cut(s) 84, 120, 122, 291, 306, 373
AcoI YGGCCR 2 cut(s) 288, 374
AcsI RAATTY 1 cut(s) 445
AfiI CCNNNNNNNGG 3 cut(s) 217, 255, 504
AgeI ACCGGT 1 cut(s) 109
AgsI TTSAA 1 cut(s) 561
AjnI CCWGG 1 cut(s) 153
AjuI GAANNNNNNNTTGG 2 cut(s) 506, 538
AluBI AGCT 2 cut(s) 349, 442
AluI AGCT 2 cut(s) 349, 442
Alw26I GTCTC 1 cut(s) 521
AoxI GGCC 5 cut(s) 288, 324, 374, 434, 471
ApeKI GCWGC 2 cut(s) 228, 346
ApoI RAATTY 1 cut(s) 445
AseI ATTAAT 1 cut(s) 411
AsiGI ACCGGT 1 cut(s) 109
AspS9I GGNCC 3 cut(s) 434, 472, 507
AsuHPI GGTGA 3 cut(s) 98, 139, 295
AvaII GGWCC 1 cut(s) 507
BbvI GCAGC 2 cut(s) 240, 358
BccI CCATC 1 cut(s) 217
BceAI ACGGC 2 cut(s) 293, 359
BciT130I CCWGG 1 cut(s) 155
BclI TGATCA 1 cut(s) 382
BcoDI GTCTC 1 cut(s) 521
BfmI CTRYAG 1 cut(s) 204
BglI GCCNNNNNGGC 1 cut(s) 343
BisI GCNGC 5 cut(s) 120, 229, 291, 347, 374
BlsI GCNGC 5 cut(s) 121, 230, 292, 348, 375
Bme1390I CCNGG 1 cut(s) 155
Bme18I GGWCC 1 cut(s) 507
BmgT120I GGNCC 3 cut(s) 434, 472, 507
BmiI GGNNCC 3 cut(s) 78, 435, 474
BmrFI CCNGG 1 cut(s) 155
BmsI GCATC 2 cut(s) 120, 496
BpmI CTGGAG 1 cut(s) 60
Bsa29I ATCGAT 1 cut(s) 490
BsaBI GATNNNNATC 1 cut(s) 396
BsaI GGTCTC 1 cut(s) 521
BsaJI CCNNGG 2 cut(s) 120, 321
BsaWI WCCGGW 2 cut(s) 109, 353
Bsc4I CCNNNNNNNGG 3 cut(s) 217, 255, 504
Bse118I RCCGGY 2 cut(s) 109, 376
Bse1I ACTGG 1 cut(s) 43
Bse8I GATNNNNATC 1 cut(s) 396
BseBI CCWGG 1 cut(s) 155
BseCI ATCGAT 1 cut(s) 490
BseDI CCNNGG 2 cut(s) 120, 321
BseGI GGATG 1 cut(s) 457
BseJI GATNNNNATC 1 cut(s) 396
BseLI CCNNNNNNNGG 3 cut(s) 217, 255, 504
BseNI ACTGG 1 cut(s) 43
BseRI GAGGAG 1 cut(s) 57
BseX3I CGGCCG 1 cut(s) 374
BseXI GCAGC 2 cut(s) 240, 358
Bsh1236I CGCG 1 cut(s) 122
Bsh1285I CGRYCG 1 cut(s) 377
BshFI GGCC 5 cut(s) 290, 326, 376, 436, 473
BshTI ACCGGT 1 cut(s) 109
BshVI ATCGAT 1 cut(s) 490
BsiEI CGRYCG 1 cut(s) 377
BsiSI CCGG 3 cut(s) 110, 354, 377
BslI CCNNNNNNNGG 3 cut(s) 217, 255, 504
BsmAI GTCTC 1 cut(s) 521
BsnI GGCC 5 cut(s) 290, 326, 376, 436, 473
Bso31I GGTCTC 1 cut(s) 521
Bsp143I GATC 3 cut(s) 382, 391, 397
BspACI CCGC 6 cut(s) 84, 120, 122, 291, 306, 373
BspANI GGCC 5 cut(s) 290, 326, 376, 436, 473
BspDI ATCGAT 1 cut(s) 490
BspFNI CGCG 1 cut(s) 122
BspHI TCATGA 1 cut(s) 385
BspLI GGNNCC 3 cut(s) 78, 435, 474
BspMAI CTGCAG 1 cut(s) 208
BspTNI GGTCTC 1 cut(s) 521
BsrFI RCCGGY 2 cut(s) 109, 376
BsrI ACTGG 1 cut(s) 43
BssAI RCCGGY 2 cut(s) 109, 376
BssECI CCNNGG 2 cut(s) 120, 321
BssMI GATC 3 cut(s) 382, 391, 397
BssT1I CCWWGG 1 cut(s) 321
Bst2UI CCWGG 1 cut(s) 155
Bst4CI ACNGT 1 cut(s) 271
Bst6I CTCTTC 3 cut(s) 248, 456, 534
BstC8I GCNNGC 1 cut(s) 378
BstDSI CCRYGG 1 cut(s) 120
BstF5I GGATG 1 cut(s) 457
BstFNI CGCG 1 cut(s) 122
BstKTI GATC 3 cut(s) 385, 394, 400
BstMAI GTCTC 1 cut(s) 521
BstMBI GATC 3 cut(s) 382, 391, 397
BstMCI CGRYCG 1 cut(s) 377
BstMWI GCNNNNNNNGC 2 cut(s) 203, 343
BstNI CCWGG 1 cut(s) 155
BstSCI CCNGG 1 cut(s) 153
BstSFI CTRYAG 1 cut(s) 204
BstUI CGCG 1 cut(s) 122
BstV1I GCAGC 2 cut(s) 240, 358
BstXI CCANNNNNNTGG 1 cut(s) 73
BstZI CGGCCG 1 cut(s) 374
Bsu15I ATCGAT 1 cut(s) 490
BsuRI GGCC 5 cut(s) 290, 326, 376, 436, 473
BsuTUI ATCGAT 1 cut(s) 490
BtgI CCRYGG 1 cut(s) 120
BtsCI GGATG 1 cut(s) 457
BtsI GCAGTG 1 cut(s) 52
BtsIMutI CAGTG 1 cut(s) 52
Cac8I GCNNGC 1 cut(s) 378
CciI TCATGA 1 cut(s) 385
Cfr10I RCCGGY 2 cut(s) 109, 376
Cfr13I GGNCC 3 cut(s) 434, 472, 507
Cfr42I CCGCGG 1 cut(s) 123
ClaI ATCGAT 1 cut(s) 490
CspAI ACCGGT 1 cut(s) 109
CviAII CATG 7 cut(s) 241, 250, 286, 328, 386, 484, 499
DpnI GATC 3 cut(s) 384, 393, 399
DpnII GATC 3 cut(s) 382, 391, 397
EaeI YGGCCR 2 cut(s) 288, 374
EagI CGGCCG 1 cut(s) 374
Eam1104I CTCTTC 3 cut(s) 248, 456, 534
EarI CTCTTC 3 cut(s) 248, 456, 534
EclXI CGGCCG 1 cut(s) 374
Eco130I CCWWGG 1 cut(s) 321
Eco31I GGTCTC 1 cut(s) 521
Eco32I GATATC 1 cut(s) 457
Eco47I GGWCC 1 cut(s) 507
Eco52I CGGCCG 1 cut(s) 374
EcoRII CCWGG 1 cut(s) 153
EcoRV GATATC 1 cut(s) 457
EcoT14I CCWWGG 1 cut(s) 321
EcoT22I ATGCAT 1 cut(s) 489
ErhI CCWWGG 1 cut(s) 321
FaeI CATG 7 cut(s) 244, 253, 289, 331, 389, 487, 502
FatI CATG 7 cut(s) 240, 249, 285, 327, 385, 483, 498
FauNDI CATATG 1 cut(s) 177
FbaI TGATCA 1 cut(s) 382
Fnu4HI GCNGC 5 cut(s) 120, 229, 291, 347, 374
FokI GGATG 1 cut(s) 464
Fsp4HI GCNGC 5 cut(s) 120, 229, 291, 347, 374
GluI GCNGC 5 cut(s) 120, 229, 291, 347, 374
GsuI CTGGAG 1 cut(s) 60
HaeIII GGCC 5 cut(s) 290, 326, 376, 436, 473
HapII CCGG 3 cut(s) 110, 354, 377
Hin1II CATG 7 cut(s) 244, 253, 289, 331, 389, 487, 502
HpaII CCGG 3 cut(s) 110, 354, 377
HphI GGTGA 3 cut(s) 98, 139, 295
Hpy166II GTNNAC 2 cut(s) 160, 235
Hpy188I TCNGA 2 cut(s) 283, 427
Hpy188III TCNNGA 3 cut(s) 95, 386, 395
Hpy8I GTNNAC 2 cut(s) 160, 235
HpyCH4III ACNGT 1 cut(s) 271
HpyCH4IV ACGT 1 cut(s) 237
HpyCH4V TGCA 5 cut(s) 21, 57, 206, 331, 487
HpyF10VI GCNNNNNNNGC 2 cut(s) 203, 343
HpySE526I ACGT 1 cut(s) 237
Hsp92II CATG 7 cut(s) 244, 253, 289, 331, 389, 487, 502
KroI GCCGGC 1 cut(s) 376
KroNI GCCGGC 1 cut(s) 378
Ksp22I TGATCA 1 cut(s) 382
KspI CCGCGG 1 cut(s) 123
Kzo9I GATC 3 cut(s) 382, 391, 397
LpnPI CCDG 9 cut(s) 24, 123, 140, 167, 169, 352, 367, 390, 489
Lsp1109I GCAGC 2 cut(s) 240, 358
LweI GCATC 2 cut(s) 120, 496
MaeII ACGT 1 cut(s) 237
MaeIII GTNAC 2 cut(s) 61, 271
MalI GATC 3 cut(s) 384, 393, 399
MboI GATC 3 cut(s) 382, 391, 397
MboII GAAGA 4 cut(s) 265, 270, 473, 551
MluCI AATT 1 cut(s) 445
MmeI TCCRAC 1 cut(s) 504
MnlI CCTC 7 cut(s) 35, 38, 118, 180, 249, 311, 535
Mph1103I ATGCAT 1 cut(s) 489
MroNI GCCGGC 1 cut(s) 376
MseI TTAA 1 cut(s) 411
MspA1I CMGCKG 1 cut(s) 122
MspI CCGG 3 cut(s) 110, 354, 377
MspR9I CCNGG 1 cut(s) 155
MvaI CCWGG 1 cut(s) 155
MvnI CGCG 1 cut(s) 122
MwoI GCNNNNNNNGC 2 cut(s) 203, 343
NaeI GCCGGC 1 cut(s) 378
NdeI CATATG 1 cut(s) 177
NdeII GATC 3 cut(s) 382, 391, 397
NgoMIV GCCGGC 1 cut(s) 376
NlaIII CATG 7 cut(s) 244, 253, 289, 331, 389, 487, 502
NlaIV GGNNCC 3 cut(s) 78, 435, 474
NmeAIII GCCGAG 1 cut(s) 449
NmuCI GTSAC 2 cut(s) 61, 271
NsiI ATGCAT 1 cut(s) 489
PagI TCATGA 1 cut(s) 385
PdiI GCCGGC 1 cut(s) 378
PflMI CCANNNNNTGG 1 cut(s) 217
PinAI ACCGGT 1 cut(s) 109
PkrI GCNGC 5 cut(s) 121, 230, 292, 348, 375
PshBI ATTAAT 1 cut(s) 411
Psp6I CCWGG 1 cut(s) 153
PspGI CCWGG 1 cut(s) 153
PspN4I GGNNCC 3 cut(s) 78, 435, 474
PspPI GGNCC 3 cut(s) 434, 472, 507
PstI CTGCAG 1 cut(s) 208
SacII CCGCGG 1 cut(s) 123
SaqAI TTAA 1 cut(s) 411
SatI GCNGC 5 cut(s) 120, 229, 291, 347, 374
Sau3AI GATC 3 cut(s) 382, 391, 397
Sau96I GGNCC 3 cut(s) 434, 472, 507
ScrFI CCNGG 1 cut(s) 155
SetI ASST 8 cut(s) 129, 191, 240, 351, 366, 444, 509, 566
SfaNI GCATC 2 cut(s) 120, 496
SfcI CTRYAG 1 cut(s) 204
Sfr303I CCGCGG 1 cut(s) 123
SgrBI CCGCGG 1 cut(s) 123
SinI GGWCC 1 cut(s) 507
Sse9I AATT 1 cut(s) 445
SsiI CCGC 6 cut(s) 84, 120, 122, 291, 306, 373
StyD4I CCNGG 1 cut(s) 153
StyI CCWWGG 1 cut(s) 321
TaaI ACNGT 1 cut(s) 271
TaiI ACGT 1 cut(s) 240
TaqI TCGA 4 cut(s) 94, 135, 459, 490
TasI AATT 1 cut(s) 445
TauI GCSGC 3 cut(s) 122, 293, 376
Tru1I TTAA 1 cut(s) 411
Tru9I TTAA 1 cut(s) 411
TscAI CASTG 1 cut(s) 59
TseFI GTSAC 2 cut(s) 61, 271
TseI GCWGC 2 cut(s) 228, 346
Tsp45I GTSAC 2 cut(s) 61, 271
TspDTI ATGAA 4 cut(s) 116, 120, 266, 472
TspRI CASTG 1 cut(s) 59
Van91I CCANNNNNTGG 1 cut(s) 217
VpaK11BI GGWCC 1 cut(s) 507
VspI ATTAAT 1 cut(s) 411
XapI RAATTY 1 cut(s) 445
Zsp2I ATGCAT 1 cut(s) 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.