MD00G1054000.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0003619
Physical Location & Seq
Forward (+)
10090947 .. 10091552
606 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1054000.v1.1.491

Sequence Viewer

Length: 606 bp
ATGGGCAACTACATTTCTTGCAGCCTAGCAACCCCAATATTGAGCAAGCATGGGAAATCTACAGCCACCAAAGTGATCATCCCAAGCGGCGAAATCAAACAATTCCATGTCCCCATAAAAGCCGCCGAGCTCATGCTCGACACCCCAAACTTCTTCCTCGTAAACACCAAGTCTCTTCGTGTCGGAAGAAGATTTTCACCTCTCAATGCCGACGAAGACTTAGATATCGGTGACGTATACGTCATGTTCCCCATGATAAGGCTCAACTCCACCGTCACAACGGCTGATATGGGGCTACTGTTCCTCAAGGCCACCGCGGCCAGCTCCGCAAGCAAGCGGGCTTCCGGGAGAAACGTGAAGGTGAGGCCGGCTGATGATCCCCATCAGGCCACGAAATTTGCTCATGATGACGGCGATGAGGGCAAGATGATTGAGAGTTGGAGGGAAAGTTCGGATAATGAGGCGGCGATGCCGAAGTTGAATTTGGATGATATAGAAGATTTCTCGGCGCCGGAATTCATGCACAGATTGTCTATGAGTAGGTCAAAGAAGCCATTGCTGGAGACCATAGCAGAAGAGCCTGCAGTTTCTGTTTCTTCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

202

Amino Acids

22.07

Weight (kDa)

6.22

Isoelectric Point (pI)

44.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 192 7.5e-36 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 239
AccB1I GGYRCC 1 cut(s) 508
AccI GTMKAC 1 cut(s) 237
AccII CGCG 1 cut(s) 317
AciI CCGC 7 cut(s) 87, 123, 315, 317, 327, 337, 464
AclWI GGATC 1 cut(s) 371
AcoI YGGCCR 1 cut(s) 318
AcsI RAATTY 3 cut(s) 395, 481, 515
AcyI GRCGYC 1 cut(s) 509
AfiI CCNNNNNNNGG 1 cut(s) 258
AgsI TTSAA 2 cut(s) 481, 600
AjuI GAANNNNNNNTTGG 2 cut(s) 467, 499
AleI CACNNNNGTG 1 cut(s) 71
AloI GAACNNNNNNTCC 2 cut(s) 433, 465
AluBI AGCT 2 cut(s) 130, 324
AluI AGCT 2 cut(s) 130, 324
Alw21I GWGCWC 1 cut(s) 132
Alw26I GTCTC 2 cut(s) 177, 557
AlwI GGATC 1 cut(s) 371
AlwNI CAGNNNCTG 1 cut(s) 590
AoxI GGCC 4 cut(s) 309, 318, 365, 387
ApeKI GCWGC 1 cut(s) 21
ApoI RAATTY 3 cut(s) 395, 481, 515
Asp700I GAANNNNTTC 1 cut(s) 193
AspLEI GCGC 1 cut(s) 511
AsuC2I CCSGG 1 cut(s) 346
AsuHPI GGTGA 3 cut(s) 189, 242, 373
BanI GGYRCC 1 cut(s) 508
BanII GRGCYC 1 cut(s) 132
BbsI GAAGAC 1 cut(s) 222
Bbv12I GWGCWC 1 cut(s) 132
BbvI GCAGC 1 cut(s) 33
BccI CCATC 1 cut(s) 390
BceAI ACGGC 2 cut(s) 297, 427
BclI TGATCA 1 cut(s) 75
BcnI CCSGG 1 cut(s) 346
BcoDI GTCTC 2 cut(s) 177, 557
BfaI CTAG 1 cut(s) 26
BfmI CTRYAG 2 cut(s) 60, 582
BfoI RGCGCY 1 cut(s) 512
BglI GCCNNNNNGGC 1 cut(s) 317
BisI GCNGC 5 cut(s) 22, 88, 123, 318, 465
BlsI GCNGC 5 cut(s) 23, 89, 124, 319, 466
Bme1390I CCNGG 1 cut(s) 346
BmiI GGNNCC 1 cut(s) 510
BmrFI CCNGG 1 cut(s) 346
BmsI GCATC 1 cut(s) 459
BpiI GAAGAC 1 cut(s) 222
BpmI CTGGAG 1 cut(s) 581
BpuEI CTTGAG 1 cut(s) 290
BpuMI CCSGG 1 cut(s) 346
BsaBI GATNNNNATC 1 cut(s) 381
BsaHI GRCGYC 1 cut(s) 509
BsaI GGTCTC 1 cut(s) 557
BsaJI CCNNGG 1 cut(s) 315
BsaXI ACNNNNNCTCC 2 cut(s) 433, 463
Bsc4I CCNNNNNNNGG 1 cut(s) 258
Bse118I RCCGGY 1 cut(s) 367
Bse3DI GCAATG 1 cut(s) 554
Bse8I GATNNNNATC 1 cut(s) 381
BseDI CCNNGG 1 cut(s) 315
BseGI GGATG 2 cut(s) 78, 493
BseJI GATNNNNATC 1 cut(s) 381
BseLI CCNNNNNNNGG 1 cut(s) 258
BseMI GCAATG 1 cut(s) 554
BseXI GCAGC 1 cut(s) 33
Bsh1236I CGCG 1 cut(s) 317
BshFI GGCC 4 cut(s) 311, 320, 367, 389
BshNI GGYRCC 1 cut(s) 508
BsiHKAI GWGCWC 1 cut(s) 132
BsiSI CCGG 3 cut(s) 345, 368, 512
BslFI GGGAC 1 cut(s) 95
BslI CCNNNNNNNGG 1 cut(s) 258
BsmAI GTCTC 2 cut(s) 177, 557
BsmFI GGGAC 1 cut(s) 95
BsnI GGCC 4 cut(s) 311, 320, 367, 389
Bso31I GGTCTC 1 cut(s) 557
Bsp1286I GDGCHC 1 cut(s) 132
Bsp143I GATC 2 cut(s) 75, 376
BspACI CCGC 7 cut(s) 87, 123, 315, 317, 327, 337, 464
BspANI GGCC 4 cut(s) 311, 320, 367, 389
BspFNI CGCG 1 cut(s) 317
BspHI TCATGA 1 cut(s) 403
BspLI GGNNCC 1 cut(s) 510
BspMAI CTGCAG 1 cut(s) 586
BspPI GGATC 1 cut(s) 371
BspQI GCTCTTC 1 cut(s) 570
BspT107I GGYRCC 1 cut(s) 508
BspTNI GGTCTC 1 cut(s) 557
BsrDI GCAATG 1 cut(s) 554
BsrFI RCCGGY 1 cut(s) 367
BssAI RCCGGY 1 cut(s) 367
BssECI CCNNGG 1 cut(s) 315
BssMI GATC 2 cut(s) 75, 376
BssNAI GTATAC 1 cut(s) 238
BssNI GRCGYC 1 cut(s) 509
Bst1107I GTATAC 1 cut(s) 238
Bst4CI ACNGT 2 cut(s) 274, 300
Bst6I CTCTTC 2 cut(s) 180, 570
BstACI GRCGYC 1 cut(s) 509
BstC8I GCNNGC 7 cut(s) 47, 322, 331, 335, 339, 369, 582
BstDEI CTNAG 1 cut(s) 220
BstDSI CCRYGG 1 cut(s) 315
BstF5I GGATG 2 cut(s) 78, 493
BstFNI CGCG 1 cut(s) 317
BstH2I RGCGCY 1 cut(s) 512
BstHHI GCGC 1 cut(s) 511
BstKTI GATC 2 cut(s) 78, 379
BstMAI GTCTC 2 cut(s) 177, 557
BstMBI GATC 2 cut(s) 75, 376
BstMWI GCNNNNNNNGC 4 cut(s) 317, 326, 330, 420
BstSCI CCNGG 1 cut(s) 344
BstSFI CTRYAG 2 cut(s) 60, 582
BstUI CGCG 1 cut(s) 317
BstV1I GCAGC 1 cut(s) 33
BstV2I GAAGAC 1 cut(s) 222
BstZ17I GTATAC 1 cut(s) 238
BsuRI GGCC 4 cut(s) 311, 320, 367, 389
BtgI CCRYGG 1 cut(s) 315
BtgZI GCGATG 2 cut(s) 429, 482
BtsCI GGATG 2 cut(s) 78, 493
Cac8I GCNNGC 7 cut(s) 47, 322, 331, 335, 339, 369, 582
CaiI CAGNNNCTG 1 cut(s) 590
CciI TCATGA 1 cut(s) 403
CfoI GCGC 1 cut(s) 511
Cfr10I RCCGGY 1 cut(s) 367
Cfr42I CCGCGG 1 cut(s) 318
CspCI CAANNNNNGTGG 2 cut(s) 379, 414
CviAII CATG 7 cut(s) 50, 107, 133, 244, 253, 404, 520
DdeI CTNAG 1 cut(s) 220
DinI GGCGCC 1 cut(s) 510
DpnI GATC 2 cut(s) 77, 378
DpnII GATC 2 cut(s) 75, 376
DrdI GACNNNNNNGTC 1 cut(s) 239
DseDI GACNNNNNNGTC 1 cut(s) 239
EaeI YGGCCR 1 cut(s) 318
Eam1104I CTCTTC 2 cut(s) 180, 570
EarI CTCTTC 2 cut(s) 180, 570
Ecl136II GAGCTC 1 cut(s) 130
Eco24I GRGCYC 1 cut(s) 132
Eco31I GGTCTC 1 cut(s) 557
Eco32I GATATC 1 cut(s) 226
Eco53kI GAGCTC 1 cut(s) 130
EcoICRI GAGCTC 1 cut(s) 130
EcoRI GAATTC 1 cut(s) 515
EcoRV GATATC 1 cut(s) 226
EcoT38I GRGCYC 1 cut(s) 132
EgeI GGCGCC 1 cut(s) 510
EheI GGCGCC 1 cut(s) 510
FaeI CATG 7 cut(s) 53, 110, 136, 247, 256, 407, 523
FaqI GGGAC 1 cut(s) 95
FatI CATG 7 cut(s) 49, 106, 132, 243, 252, 403, 519
FauI CCCGC 1 cut(s) 330
FbaI TGATCA 1 cut(s) 75
FblI GTMKAC 1 cut(s) 237
Fnu4HI GCNGC 5 cut(s) 22, 88, 123, 318, 465
FokI GGATG 2 cut(s) 65, 500
FriOI GRGCYC 1 cut(s) 132
Fsp4HI GCNGC 5 cut(s) 22, 88, 123, 318, 465
FspBI CTAG 1 cut(s) 26
GlaI GCGC 1 cut(s) 510
GluI GCNGC 5 cut(s) 22, 88, 123, 318, 465
GsuI CTGGAG 1 cut(s) 581
HaeII RGCGCY 1 cut(s) 512
HaeIII GGCC 4 cut(s) 311, 320, 367, 389
HapII CCGG 3 cut(s) 345, 368, 512
HhaI GCGC 1 cut(s) 511
Hin1I GRCGYC 1 cut(s) 509
Hin1II CATG 7 cut(s) 53, 110, 136, 247, 256, 407, 523
Hin6I GCGC 1 cut(s) 509
HinP1I GCGC 1 cut(s) 509
HpaII CCGG 3 cut(s) 345, 368, 512
HphI GGTGA 3 cut(s) 189, 242, 373
Hpy166II GTNNAC 2 cut(s) 163, 238
Hpy188I TCNGA 2 cut(s) 185, 454
Hpy188III TCNNGA 1 cut(s) 404
Hpy8I GTNNAC 2 cut(s) 163, 238
Hpy99I CGWCG 1 cut(s) 215
HpyAV CCTTC 1 cut(s) 352
HpyCH4III ACNGT 2 cut(s) 274, 300
HpyCH4IV ACGT 3 cut(s) 234, 240, 354
HpyCH4V TGCA 3 cut(s) 21, 523, 584
HpyF10VI GCNNNNNNNGC 4 cut(s) 317, 326, 330, 420
HpyF3I CTNAG 1 cut(s) 220
HpySE526I ACGT 3 cut(s) 234, 240, 354
Hsp92I GRCGYC 1 cut(s) 509
Hsp92II CATG 7 cut(s) 53, 110, 136, 247, 256, 407, 523
HspAI GCGC 1 cut(s) 509
KasI GGCGCC 1 cut(s) 508
KroI GCCGGC 1 cut(s) 367
KroNI GCCGGC 1 cut(s) 369
Ksp22I TGATCA 1 cut(s) 75
KspI CCGCGG 1 cut(s) 318
Kzo9I GATC 2 cut(s) 75, 376
LguI GCTCTTC 1 cut(s) 570
LmnI GCTCC 1 cut(s) 329
LpnPI CCDG 7 cut(s) 334, 358, 371, 381, 525, 545, 594
Lsp1109I GCAGC 1 cut(s) 33
LweI GCATC 1 cut(s) 459
MaeI CTAG 1 cut(s) 26
MaeII ACGT 3 cut(s) 234, 240, 354
MaeIII GTNAC 2 cut(s) 230, 274
MalI GATC 2 cut(s) 77, 378
MboI GATC 2 cut(s) 75, 376
MboII GAAGA 8 cut(s) 145, 167, 198, 201, 227, 509, 587, 588
MhlI GDGCHC 1 cut(s) 132
MluCI AATT 4 cut(s) 101, 395, 481, 515
Mly113I GGCGCC 1 cut(s) 509
MmeI TCCRAC 2 cut(s) 163, 419
MnlI CCTC 7 cut(s) 167, 210, 314, 357, 412, 435, 454
MroNI GCCGGC 1 cut(s) 367
MroXI GAANNNNTTC 1 cut(s) 193
MslI CAYNNNNRTG 1 cut(s) 71
MspA1I CMGCKG 1 cut(s) 317
MspI CCGG 3 cut(s) 345, 368, 512
MspR9I CCNGG 1 cut(s) 346
MvnI CGCG 1 cut(s) 317
MwoI GCNNNNNNNGC 4 cut(s) 317, 326, 330, 420
NaeI GCCGGC 1 cut(s) 369
NarI GGCGCC 1 cut(s) 509
NciI CCSGG 1 cut(s) 346
NdeII GATC 2 cut(s) 75, 376
NgoMIV GCCGGC 1 cut(s) 367
NlaIII CATG 7 cut(s) 53, 110, 136, 247, 256, 407, 523
NlaIV GGNNCC 1 cut(s) 510
NmeAIII GCCGAG 2 cut(s) 151, 485
NmuCI GTSAC 2 cut(s) 230, 274
OliI CACNNNNGTG 1 cut(s) 71
PagI TCATGA 1 cut(s) 403
PciSI GCTCTTC 1 cut(s) 570
PdiI GCCGGC 1 cut(s) 369
PdmI GAANNNNTTC 1 cut(s) 193
PfoI TCCNGGA 1 cut(s) 344
PkrI GCNGC 5 cut(s) 23, 89, 124, 319, 466
PluTI GGCGCC 1 cut(s) 512
Psp124BI GAGCTC 1 cut(s) 132
PspN4I GGNNCC 1 cut(s) 510
PstI CTGCAG 1 cut(s) 586
PstNI CAGNNNCTG 1 cut(s) 590
RseI CAYNNNNRTG 1 cut(s) 71
SacI GAGCTC 1 cut(s) 132
SacII CCGCGG 1 cut(s) 318
SapI GCTCTTC 1 cut(s) 570
SatI GCNGC 5 cut(s) 22, 88, 123, 318, 465
Sau3AI GATC 2 cut(s) 75, 376
ScrFI CCNGG 1 cut(s) 346
SduI GDGCHC 1 cut(s) 132
SetI ASST 8 cut(s) 132, 202, 237, 243, 326, 357, 363, 545
SfaNI GCATC 1 cut(s) 459
SfcI CTRYAG 2 cut(s) 60, 582
SfiI GGCCNNNNNGGCC 1 cut(s) 317
SfoI GGCGCC 1 cut(s) 510
Sfr303I CCGCGG 1 cut(s) 318
SgrBI CCGCGG 1 cut(s) 318
SmiMI CAYNNNNRTG 1 cut(s) 71
SmlI CTYRAG 1 cut(s) 305
SmoI CTYRAG 1 cut(s) 305
Sse9I AATT 4 cut(s) 101, 395, 481, 515
SsiI CCGC 7 cut(s) 87, 123, 315, 317, 327, 337, 464
SspDI GGCGCC 1 cut(s) 508
SspI AATATT 1 cut(s) 39
SspMI CTAG 1 cut(s) 26
SstI GAGCTC 1 cut(s) 132
StyD4I CCNGG 1 cut(s) 344
TaaI ACNGT 2 cut(s) 274, 300
TaiI ACGT 3 cut(s) 237, 243, 357
TaqI TCGA 1 cut(s) 138
TasI AATT 4 cut(s) 101, 395, 481, 515
TauI GCSGC 4 cut(s) 90, 125, 320, 467
TseFI GTSAC 2 cut(s) 230, 274
TseI GCWGC 1 cut(s) 21
Tsp45I GTSAC 2 cut(s) 230, 274
TspDTI ATGAA 1 cut(s) 508
XapI RAATTY 3 cut(s) 395, 481, 515
XmiI GTMKAC 1 cut(s) 237
XmnI GAANNNNTTC 1 cut(s) 193
XspI CTAG 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.