FvH4_4g26170

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0003619
Physical Location & Seq
Reverse (-)
27680690 .. 27681235
546 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g26170.t1

Sequence Viewer

Length: 546 bp
ATGGGGAACTACATCTCATGCACTCTAGCCACGCCTCTCATCAAGAGCACCAAGGCGGCGCGTGTGATCTTCCCCACCGGCGAAGTACGTCAGTTCCGTGAACCCATCAAGGCCGCCGAGCTCATGCTCGAGTCTCCAAACACGTTTCTAGCCAACTCCAAGTCTCTCCACATCGGCCGCCGCTTCTCCGCCCTCGCCGCCGACGAGGAGCTCGAGTTCGGCAACGTCTACATCATGTTCCCCATGAAACGGCTCAGCTCCATTGTCACCGCCGCCGACATGACCATCTTCTTCATCGCGGCCAACTCCGCCGCCAAGCGTATTTCCGGCGGGAAGGTCCGGATCTTGCCGGAGGCTCAAGCGTTCCAAAACAAGGACGAGGAGAATAATCAAAGCAGTGACCGAGAAGGTGAAGCTCCGGCGAGGCTGAGCTTGGACGGAGTTGAGGGGTTGCAAGTGGCGGAGTTCCAGTATAGGTTAGCTGCGTGTAGGTCAAGAAAGCCATTGTTGGAGACCATCAAAGAGGAGCCCATTTTTTCTAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

182

Amino Acids

20.09

Weight (kDa)

8.58

Isoelectric Point (pI)

46.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 175 2.5e-39 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 228
AccII CGCG 2 cut(s) 61, 299
AccIII TCCGGA 1 cut(s) 339
AclWI GGATC 1 cut(s) 350
AcoI YGGCCR 2 cut(s) 175, 300
AfaI GTAC 1 cut(s) 87
AfiI CCNNNNNNNGG 2 cut(s) 249, 373
AflIII ACRYGT 1 cut(s) 141
AloI GAACNNNNNNTCC 2 cut(s) 200, 232
AluBI AGCT 6 cut(s) 121, 211, 258, 416, 432, 482
AluI AGCT 6 cut(s) 121, 211, 258, 416, 432, 482
Alw21I GWGCWC 3 cut(s) 50, 123, 213
Alw26I GTCTC 3 cut(s) 138, 168, 506
AlwI GGATC 1 cut(s) 350
Ama87I CYCGRG 2 cut(s) 128, 212
Aor13HI TCCGGA 1 cut(s) 339
AoxI GGCC 3 cut(s) 111, 175, 300
ApeKI GCWGC 1 cut(s) 482
AspLEI GCGC 1 cut(s) 61
AspS9I GGNCC 1 cut(s) 337
AsuHPI GGTGA 2 cut(s) 259, 422
AvaI CYCGRG 2 cut(s) 128, 212
AvaII GGWCC 1 cut(s) 337
BanII GRGCYC 3 cut(s) 123, 213, 531
Bbv12I GWGCWC 3 cut(s) 50, 123, 213
BbvI GCAGC 1 cut(s) 469
BccI CCATC 3 cut(s) 113, 293, 524
BceAI ACGGC 1 cut(s) 266
BcoDI GTCTC 3 cut(s) 138, 168, 506
BfaI CTAG 3 cut(s) 26, 149, 540
BisI GCNGC 9 cut(s) 57, 114, 178, 181, 198, 273, 300, 312, 483
BlpI GCTNAGC 2 cut(s) 254, 428
BlsI GCNGC 9 cut(s) 58, 115, 179, 182, 199, 274, 301, 313, 484
Bme18I GGWCC 1 cut(s) 337
BmeT110I CYCGRG 2 cut(s) 128, 212
BmgT120I GGNCC 1 cut(s) 337
BmiI GGNNCC 1 cut(s) 528
Bpu1102I GCTNAGC 2 cut(s) 254, 428
BpuEI CTTGAG 1 cut(s) 342
BsaI GGTCTC 1 cut(s) 506
BsaJI CCNNGG 1 cut(s) 51
BsaWI WCCGGW 1 cut(s) 339
BsaXI ACNNNNNCTCC 4 cut(s) 200, 230, 455, 485
Bsc4I CCNNNNNNNGG 2 cut(s) 249, 373
Bse118I RCCGGY 1 cut(s) 77
Bse1I ACTGG 1 cut(s) 469
BseAI TCCGGA 1 cut(s) 339
BseDI CCNNGG 1 cut(s) 51
BseLI CCNNNNNNNGG 2 cut(s) 249, 373
BseMII CTCAG 2 cut(s) 268, 419
BseNI ACTGG 1 cut(s) 469
BseRI GAGGAG 3 cut(s) 221, 395, 539
BseX3I CGGCCG 1 cut(s) 175
BseXI GCAGC 1 cut(s) 469
Bsh1236I CGCG 2 cut(s) 61, 299
Bsh1285I CGRYCG 1 cut(s) 178
BshFI GGCC 3 cut(s) 113, 177, 302
BsiEI CGRYCG 1 cut(s) 178
BsiHKAI GWGCWC 3 cut(s) 50, 123, 213
BsiHKCI CYCGRG 2 cut(s) 128, 212
BsiSI CCGG 5 cut(s) 78, 327, 340, 350, 419
BslI CCNNNNNNNGG 2 cut(s) 249, 373
BsmAI GTCTC 3 cut(s) 138, 168, 506
BsnI GGCC 3 cut(s) 113, 177, 302
Bso31I GGTCTC 1 cut(s) 506
BsoBI CYCGRG 2 cut(s) 128, 212
Bsp1286I GDGCHC 4 cut(s) 50, 123, 213, 531
Bsp13I TCCGGA 1 cut(s) 339
Bsp143I GATC 2 cut(s) 66, 342
Bsp1720I GCTNAGC 2 cut(s) 254, 428
BspANI GGCC 3 cut(s) 113, 177, 302
BspCNI CTCAG 2 cut(s) 267, 420
BspEI TCCGGA 1 cut(s) 339
BspFNI CGCG 2 cut(s) 61, 299
BspLI GGNNCC 1 cut(s) 528
BspPI GGATC 1 cut(s) 350
BspTNI GGTCTC 1 cut(s) 506
BsrFI RCCGGY 1 cut(s) 77
BsrI ACTGG 1 cut(s) 469
BssAI RCCGGY 1 cut(s) 77
BssECI CCNNGG 1 cut(s) 51
BssMI GATC 2 cut(s) 66, 342
BssT1I CCWWGG 1 cut(s) 51
BstDEI CTNAG 2 cut(s) 254, 428
BstFNI CGCG 2 cut(s) 61, 299
BstHHI GCGC 1 cut(s) 61
BstKTI GATC 2 cut(s) 69, 345
BstMAI GTCTC 3 cut(s) 138, 168, 506
BstMBI GATC 2 cut(s) 66, 342
BstMCI CGRYCG 1 cut(s) 178
BstMWI GCNNNNNNNGC 2 cut(s) 197, 308
BstUI CGCG 2 cut(s) 61, 299
BstV1I GCAGC 1 cut(s) 469
BstX2I RGATCY 1 cut(s) 342
BstYI RGATCY 1 cut(s) 342
BstZI CGGCCG 1 cut(s) 175
BsuRI GGCC 3 cut(s) 113, 177, 302
BtgZI GCGATG 1 cut(s) 280
BtsI GCAGTG 1 cut(s) 403
BtsIMutI CAGTG 1 cut(s) 403
CfoI GCGC 1 cut(s) 61
Cfr10I RCCGGY 1 cut(s) 77
Cfr13I GGNCC 1 cut(s) 337
Csp6I GTAC 1 cut(s) 86
CviAII CATG 5 cut(s) 18, 124, 235, 244, 280
CviQI GTAC 1 cut(s) 86
DdeI CTNAG 2 cut(s) 254, 428
DpnI GATC 2 cut(s) 68, 344
DpnII GATC 2 cut(s) 66, 342
EaeI YGGCCR 2 cut(s) 175, 300
EagI CGGCCG 1 cut(s) 175
EciI GGCGGA 3 cut(s) 178, 298, 476
Ecl136II GAGCTC 2 cut(s) 121, 211
EclXI CGGCCG 1 cut(s) 175
Eco130I CCWWGG 1 cut(s) 51
Eco24I GRGCYC 3 cut(s) 123, 213, 531
Eco31I GGTCTC 1 cut(s) 506
Eco47I GGWCC 1 cut(s) 337
Eco52I CGGCCG 1 cut(s) 175
Eco53kI GAGCTC 2 cut(s) 121, 211
Eco88I CYCGRG 2 cut(s) 128, 212
EcoICRI GAGCTC 2 cut(s) 121, 211
EcoT14I CCWWGG 1 cut(s) 51
EcoT38I GRGCYC 3 cut(s) 123, 213, 531
ErhI CCWWGG 1 cut(s) 51
FaeI CATG 5 cut(s) 21, 127, 238, 247, 283
FaiI YATR 6 cut(s) 19, 125, 236, 245, 281, 474
FatI CATG 5 cut(s) 17, 123, 234, 243, 279
FauI CCCGC 1 cut(s) 323
FblI GTMKAC 1 cut(s) 228
Fnu4HI GCNGC 9 cut(s) 57, 114, 178, 181, 198, 273, 300, 312, 483
FriOI GRGCYC 3 cut(s) 123, 213, 531
Fsp4HI GCNGC 9 cut(s) 57, 114, 178, 181, 198, 273, 300, 312, 483
FspBI CTAG 3 cut(s) 26, 149, 540
GlaI GCGC 1 cut(s) 60
GluI GCNGC 9 cut(s) 57, 114, 178, 181, 198, 273, 300, 312, 483
HaeIII GGCC 3 cut(s) 113, 177, 302
HapII CCGG 5 cut(s) 78, 327, 340, 350, 419
HhaI GCGC 1 cut(s) 61
Hin1II CATG 5 cut(s) 21, 127, 238, 247, 283
Hin6I GCGC 1 cut(s) 59
HinP1I GCGC 1 cut(s) 59
HinfI GANTC 1 cut(s) 131
HpaII CCGG 5 cut(s) 78, 327, 340, 350, 419
HphI GGTGA 2 cut(s) 259, 422
Hpy166II GTNNAC 2 cut(s) 101, 229
Hpy188III TCNNGA 4 cut(s) 43, 340, 495, 540
Hpy8I GTNNAC 2 cut(s) 101, 229
Hpy99I CGWCG 1 cut(s) 206
HpyAV CCTTC 2 cut(s) 328, 401
HpyCH4IV ACGT 3 cut(s) 88, 143, 225
HpyCH4V TGCA 2 cut(s) 21, 454
HpyF10VI GCNNNNNNNGC 2 cut(s) 197, 308
HpyF3I CTNAG 2 cut(s) 254, 428
HpySE526I ACGT 3 cut(s) 88, 143, 225
Hsp92II CATG 5 cut(s) 21, 127, 238, 247, 283
HspAI GCGC 1 cut(s) 59
Kpn2I TCCGGA 1 cut(s) 339
Kzo9I GATC 2 cut(s) 66, 342
LmnI GCTCC 4 cut(s) 208, 263, 421, 526
LpnPI CCDG 6 cut(s) 91, 340, 353, 363, 432, 482
Lsp1109I GCAGC 1 cut(s) 469
MaeI CTAG 3 cut(s) 26, 149, 540
MaeII ACGT 3 cut(s) 88, 143, 225
MaeIII GTNAC 2 cut(s) 265, 398
MalI GATC 2 cut(s) 68, 344
MboI GATC 2 cut(s) 66, 342
MboII GAAGA 3 cut(s) 61, 280, 283
MflI RGATCY 1 cut(s) 342
MhlI GDGCHC 4 cut(s) 50, 123, 213, 531
MlyI GAGTC 1 cut(s) 140
MmeI TCCRAC 1 cut(s) 489
MnlI CCTC 8 cut(s) 45, 199, 203, 346, 373, 417, 439, 517
MroI TCCGGA 1 cut(s) 339
MspI CCGG 5 cut(s) 78, 327, 340, 350, 419
MvnI CGCG 2 cut(s) 61, 299
MwoI GCNNNNNNNGC 2 cut(s) 197, 308
NdeII GATC 2 cut(s) 66, 342
NlaIII CATG 5 cut(s) 21, 127, 238, 247, 283
NlaIV GGNNCC 1 cut(s) 528
NmeAIII GCCGAG 1 cut(s) 142
NmuCI GTSAC 2 cut(s) 265, 398
PaeR7I CTCGAG 2 cut(s) 128, 212
PcsI WCGNNNNNNNCGW 3 cut(s) 94, 201, 210
PkrI GCNGC 9 cut(s) 58, 115, 179, 182, 199, 274, 301, 313, 484
PleI GAGTC 1 cut(s) 139
PpsI GAGTC 1 cut(s) 139
Psp124BI GAGCTC 2 cut(s) 123, 213
PspN4I GGNNCC 1 cut(s) 528
PspPI GGNCC 1 cut(s) 337
PspXI VCTCGAGB 2 cut(s) 128, 212
PsuI RGATCY 1 cut(s) 342
RsaI GTAC 1 cut(s) 87
RsaNI GTAC 1 cut(s) 86
SacI GAGCTC 2 cut(s) 123, 213
SatI GCNGC 9 cut(s) 57, 114, 178, 181, 198, 273, 300, 312, 483
Sau3AI GATC 2 cut(s) 66, 342
Sau96I GGNCC 1 cut(s) 337
SchI GAGTC 1 cut(s) 140
SduI GDGCHC 4 cut(s) 50, 123, 213, 531
Sfr274I CTCGAG 2 cut(s) 128, 212
SgrAI CRCCGGYG 1 cut(s) 77
SinI GGWCC 1 cut(s) 337
SlaI CTCGAG 2 cut(s) 128, 212
SmlI CTYRAG 3 cut(s) 128, 212, 357
SmoI CTYRAG 3 cut(s) 128, 212, 357
SspMI CTAG 3 cut(s) 26, 149, 540
SstI GAGCTC 2 cut(s) 123, 213
StyI CCWWGG 1 cut(s) 51
TaiI ACGT 3 cut(s) 91, 146, 228
TaqI TCGA 2 cut(s) 129, 213
TaqII GACCGA 1 cut(s) 417
TauI GCSGC 8 cut(s) 59, 116, 180, 183, 200, 275, 302, 314
TscAI CASTG 1 cut(s) 403
TseFI GTSAC 2 cut(s) 265, 398
TseI GCWGC 1 cut(s) 482
Tsp45I GTSAC 2 cut(s) 265, 398
TspDTI ATGAA 2 cut(s) 260, 283
TspGWI ACGGA 2 cut(s) 86, 453
TspRI CASTG 1 cut(s) 403
VpaK11BI GGWCC 1 cut(s) 337
XbaI TCTAGA 1 cut(s) 539
XhoI CTCGAG 2 cut(s) 128, 212
XmiI GTMKAC 1 cut(s) 228
XspI CTAG 3 cut(s) 26, 149, 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.