MD13G1028700.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0003619
Physical Location & Seq
Reverse (-)
2043542 .. 2044119
578 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1028700.v1.1.491

Sequence Viewer

Length: 534 bp
ATGATCAAGAGCTCCAAATCAGCGAGGGTGATCTTCCCCAACGGCGACGTTCGGCAGTTCCGGGAGATGGTTAAGGCGGCGGAGCTCATGCTGGAGTGTCCCAACACCTTCTTGGCCAACTCGAGGTCTCTCCACATCGGCAGGAGGTTCTCCGCCCTCAGCGCCGACGAGGAGTTGGAGTTTGGGAACGTGTACATCATGTTTCCAATGAGGAGGCTCAGTTCCATAGTCACGGCGGCTGACATGGCGGTGTTCTTCATGGCGGCCAACTCCGCCGCCAAGCGTATATCAGGCGGACAGGTTAGGATCTTGCCGGAGAGCGGCAGTGGAGGAGGAGAAGTGGGCGCGGTGGAAAGTGGGAAAGAAAATCTTGGCGATCAAGATCATGAAGGTGATCATCAAGGTCCATTGCGGTTGAGCTTGGACGGAGTAGAGGGGTTGCAAGTGGCGGAGTTTCAGTACAGATTAGCTGCGTGTAGGTCAAGGAAGCCGGTGTTGGAAACCATCAAAGAAGAGCAGATTTGTTCTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

178

Amino Acids

19.39

Weight (kDa)

6.33

Isoelectric Point (pI)

48.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 4 - 171 3.1e-33 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 321
AccII CGCG 1 cut(s) 347
AclWI GGATC 1 cut(s) 314
AcoI YGGCCR 2 cut(s) 114, 264
AfaI GTAC 2 cut(s) 194, 461
AfiI CCNNNNNNNGG 3 cut(s) 67, 123, 320
AflIII ACRYGT 1 cut(s) 189
AjuI GAANNNNNNNTTGG 2 cut(s) 479, 511
AloI GAACNNNNNNTCC 2 cut(s) 205, 237
AluBI AGCT 4 cut(s) 12, 85, 420, 470
AluI AGCT 4 cut(s) 12, 85, 420, 470
Alw21I GWGCWC 2 cut(s) 14, 87
Alw26I GTCTC 1 cut(s) 132
AlwI GGATC 1 cut(s) 314
Ama87I CYCGRG 1 cut(s) 121
AoxI GGCC 2 cut(s) 114, 264
ApeKI GCWGC 1 cut(s) 470
AspLEI GCGC 2 cut(s) 164, 347
AspS9I GGNCC 1 cut(s) 404
AsuC2I CCSGG 1 cut(s) 62
AsuHPI GGTGA 2 cut(s) 40, 404
AvaI CYCGRG 1 cut(s) 121
AvaII GGWCC 1 cut(s) 404
BalI TGGCCA 1 cut(s) 116
BanII GRGCYC 2 cut(s) 14, 87
Bbv12I GWGCWC 2 cut(s) 14, 87
BbvCI CCTCAGC 1 cut(s) 158
BbvI GCAGC 1 cut(s) 457
BccI CCATC 2 cut(s) 61, 512
BceAI ACGGC 2 cut(s) 58, 249
BclI TGATCA 2 cut(s) 3, 394
BcnI CCSGG 1 cut(s) 62
BcoDI GTCTC 1 cut(s) 132
BfaI CTAG 1 cut(s) 528
BfoI RGCGCY 1 cut(s) 165
BisI GCNGC 6 cut(s) 78, 237, 264, 276, 322, 471
BlsI GCNGC 6 cut(s) 79, 238, 265, 277, 323, 472
Bme1390I CCNGG 1 cut(s) 62
Bme18I GGWCC 1 cut(s) 404
BmeT110I CYCGRG 1 cut(s) 121
BmgT120I GGNCC 1 cut(s) 404
BmrFI CCNGG 1 cut(s) 62
BpmI CTGGAG 1 cut(s) 113
Bpu10I CCTNAGC 1 cut(s) 158
BpuMI CCSGG 1 cut(s) 62
BsaBI GATNNNNATC 1 cut(s) 381
BsaI GGTCTC 1 cut(s) 132
BsaXI ACNNNNNCTCC 8 cut(s) 164, 194, 205, 235, 324, 354, 443, 473
Bsc4I CCNNNNNNNGG 3 cut(s) 67, 123, 320
Bse118I RCCGGY 1 cut(s) 490
Bse3DI GCAATG 1 cut(s) 407
Bse8I GATNNNNATC 1 cut(s) 381
BseJI GATNNNNATC 1 cut(s) 381
BseLI CCNNNNNNNGG 3 cut(s) 67, 123, 320
BseMI GCAATG 1 cut(s) 407
BseMII CTCAG 2 cut(s) 172, 232
BseRI GAGGAG 4 cut(s) 185, 226, 345, 348
BseXI GCAGC 1 cut(s) 457
Bsh1236I CGCG 1 cut(s) 347
BshFI GGCC 2 cut(s) 116, 266
BsiHKAI GWGCWC 2 cut(s) 14, 87
BsiHKCI CYCGRG 1 cut(s) 121
BsiSI CCGG 3 cut(s) 61, 314, 491
BslFI GGGAC 1 cut(s) 84
BslI CCNNNNNNNGG 3 cut(s) 67, 123, 320
BsmAI GTCTC 1 cut(s) 132
BsmFI GGGAC 1 cut(s) 84
BsnI GGCC 2 cut(s) 116, 266
Bso31I GGTCTC 1 cut(s) 132
BsoBI CYCGRG 1 cut(s) 121
Bsp1286I GDGCHC 2 cut(s) 14, 87
Bsp1407I TGTACA 1 cut(s) 192
Bsp143I GATC 6 cut(s) 3, 30, 306, 376, 382, 394
BspANI GGCC 2 cut(s) 116, 266
BspCNI CTCAG 2 cut(s) 171, 231
BspFNI CGCG 1 cut(s) 347
BspHI TCATGA 1 cut(s) 385
BspPI GGATC 1 cut(s) 314
BspQI GCTCTTC 1 cut(s) 507
BspTNI GGTCTC 1 cut(s) 132
BsrBI CCGCTC 1 cut(s) 321
BsrDI GCAATG 1 cut(s) 407
BsrFI RCCGGY 1 cut(s) 490
BsrGI TGTACA 1 cut(s) 192
BssAI RCCGGY 1 cut(s) 490
BssMI GATC 6 cut(s) 3, 30, 306, 376, 382, 394
Bst6I CTCTTC 1 cut(s) 507
BstAUI TGTACA 1 cut(s) 192
BstDEI CTNAG 2 cut(s) 158, 218
BstFNI CGCG 1 cut(s) 347
BstH2I RGCGCY 1 cut(s) 165
BstHHI GCGC 2 cut(s) 164, 347
BstKTI GATC 6 cut(s) 6, 33, 309, 379, 385, 397
BstMAI GTCTC 1 cut(s) 132
BstMBI GATC 6 cut(s) 3, 30, 306, 376, 382, 394
BstMWI GCNNNNNNNGC 3 cut(s) 161, 245, 272
BstSCI CCNGG 1 cut(s) 60
BstUI CGCG 1 cut(s) 347
BstV1I GCAGC 1 cut(s) 457
BstX2I RGATCY 1 cut(s) 306
BstYI RGATCY 1 cut(s) 306
BsuRI GGCC 2 cut(s) 116, 266
BtsI GCAGTG 1 cut(s) 331
BtsIMutI CAGTG 1 cut(s) 331
CciI TCATGA 1 cut(s) 385
CfoI GCGC 2 cut(s) 164, 347
Cfr10I RCCGGY 1 cut(s) 490
Cfr13I GGNCC 1 cut(s) 404
Csp6I GTAC 2 cut(s) 193, 460
CviAII CATG 5 cut(s) 88, 199, 244, 259, 386
CviJI RGCY 9 cut(s) 12, 85, 116, 217, 239, 266, 420, 470, 490
CviKI_1 RGCY 9 cut(s) 12, 85, 116, 217, 239, 266, 420, 470, 490
CviQI GTAC 2 cut(s) 193, 460
DdeI CTNAG 2 cut(s) 158, 218
DpnI GATC 6 cut(s) 5, 32, 308, 378, 384, 396
DpnII GATC 6 cut(s) 3, 30, 306, 376, 382, 394
EaeI YGGCCR 2 cut(s) 114, 264
Eam1104I CTCTTC 1 cut(s) 507
EarI CTCTTC 1 cut(s) 507
EciI GGCGGA 5 cut(s) 95, 142, 262, 309, 464
Ecl136II GAGCTC 2 cut(s) 12, 85
Eco24I GRGCYC 2 cut(s) 14, 87
Eco31I GGTCTC 1 cut(s) 132
Eco47I GGWCC 1 cut(s) 404
Eco53kI GAGCTC 2 cut(s) 12, 85
Eco88I CYCGRG 1 cut(s) 121
EcoICRI GAGCTC 2 cut(s) 12, 85
EcoT38I GRGCYC 2 cut(s) 14, 87
FaeI CATG 5 cut(s) 91, 202, 247, 262, 389
FaiI YATR 7 cut(s) 89, 200, 227, 245, 260, 287, 387
FalI AAGNNNNNCTT 2 cut(s) 354, 386
FaqI GGGAC 1 cut(s) 84
FatI CATG 5 cut(s) 87, 198, 243, 258, 385
FbaI TGATCA 2 cut(s) 3, 394
Fnu4HI GCNGC 6 cut(s) 78, 237, 264, 276, 322, 471
FriOI GRGCYC 2 cut(s) 14, 87
Fsp4HI GCNGC 6 cut(s) 78, 237, 264, 276, 322, 471
FspBI CTAG 1 cut(s) 528
GlaI GCGC 2 cut(s) 163, 346
GluI GCNGC 6 cut(s) 78, 237, 264, 276, 322, 471
GsuI CTGGAG 1 cut(s) 113
HaeII RGCGCY 1 cut(s) 165
HaeIII GGCC 2 cut(s) 116, 266
HapII CCGG 3 cut(s) 61, 314, 491
HhaI GCGC 2 cut(s) 164, 347
Hin1II CATG 5 cut(s) 91, 202, 247, 262, 389
Hin6I GCGC 2 cut(s) 162, 345
HinP1I GCGC 2 cut(s) 162, 345
HpaII CCGG 3 cut(s) 61, 314, 491
HphI GGTGA 2 cut(s) 40, 404
Hpy166II GTNNAC 1 cut(s) 193
Hpy188III TCNNGA 4 cut(s) 7, 380, 386, 528
Hpy8I GTNNAC 1 cut(s) 193
Hpy99I CGWCG 2 cut(s) 50, 170
HpyAV CCTTC 2 cut(s) 118, 383
HpyCH4IV ACGT 2 cut(s) 48, 189
HpyCH4V TGCA 1 cut(s) 442
HpyF10VI GCNNNNNNNGC 3 cut(s) 161, 245, 272
HpyF3I CTNAG 2 cut(s) 158, 218
HpySE526I ACGT 2 cut(s) 48, 189
Hsp92II CATG 5 cut(s) 91, 202, 247, 262, 389
HspAI GCGC 2 cut(s) 162, 345
Ksp22I TGATCA 2 cut(s) 3, 394
Kzo9I GATC 6 cut(s) 3, 30, 306, 376, 382, 394
LguI GCTCTTC 1 cut(s) 507
LmnI GCTCC 2 cut(s) 17, 82
LpnPI CCDG 7 cut(s) 74, 77, 127, 276, 284, 327, 504
Lsp1109I GCAGC 1 cut(s) 457
MaeI CTAG 1 cut(s) 528
MaeII ACGT 2 cut(s) 48, 189
MaeIII GTNAC 1 cut(s) 229
MalI GATC 6 cut(s) 5, 32, 308, 378, 384, 396
MbiI CCGCTC 1 cut(s) 321
MboI GATC 6 cut(s) 3, 30, 306, 376, 382, 394
MboII GAAGA 3 cut(s) 25, 247, 524
MflI RGATCY 1 cut(s) 306
MhlI GDGCHC 2 cut(s) 14, 87
MlsI TGGCCA 1 cut(s) 116
MluNI TGGCCA 1 cut(s) 116
MmeI TCCRAC 2 cut(s) 156, 477
Mox20I TGGCCA 1 cut(s) 116
MscI TGGCCA 1 cut(s) 116
MseI TTAA 1 cut(s) 72
MslI CAYNNNNRTG 2 cut(s) 248, 390
Msp20I TGGCCA 1 cut(s) 116
MspI CCGG 3 cut(s) 61, 314, 491
MspR9I CCNGG 1 cut(s) 62
MvnI CGCG 1 cut(s) 347
MwoI GCNNNNNNNGC 3 cut(s) 161, 245, 272
NciI CCSGG 1 cut(s) 62
NdeII GATC 6 cut(s) 3, 30, 306, 376, 382, 394
NlaIII CATG 5 cut(s) 91, 202, 247, 262, 389
NmuCI GTSAC 1 cut(s) 229
PaeR7I CTCGAG 1 cut(s) 121
PagI TCATGA 1 cut(s) 385
PciSI GCTCTTC 1 cut(s) 507
PfoI TCCNGGA 1 cut(s) 60
PkrI GCNGC 6 cut(s) 79, 238, 265, 277, 323, 472
Psp124BI GAGCTC 2 cut(s) 14, 87
PspPI GGNCC 1 cut(s) 404
PspXI VCTCGAGB 1 cut(s) 121
PsuI RGATCY 1 cut(s) 306
RsaI GTAC 2 cut(s) 194, 461
RsaNI GTAC 2 cut(s) 193, 460
RseI CAYNNNNRTG 2 cut(s) 248, 390
SacI GAGCTC 2 cut(s) 14, 87
SapI GCTCTTC 1 cut(s) 507
SaqAI TTAA 1 cut(s) 72
SatI GCNGC 6 cut(s) 78, 237, 264, 276, 322, 471
Sau3AI GATC 6 cut(s) 3, 30, 306, 376, 382, 394
Sau96I GGNCC 1 cut(s) 404
ScrFI CCNGG 1 cut(s) 62
SduI GDGCHC 2 cut(s) 14, 87
Sfr274I CTCGAG 1 cut(s) 121
SinI GGWCC 1 cut(s) 404
SlaI CTCGAG 1 cut(s) 121
SmiMI CAYNNNNRTG 2 cut(s) 248, 390
SmlI CTYRAG 1 cut(s) 121
SmoI CTYRAG 1 cut(s) 121
SspMI CTAG 1 cut(s) 528
SstI GAGCTC 2 cut(s) 14, 87
StyD4I CCNGG 1 cut(s) 60
TaiI ACGT 2 cut(s) 51, 192
TaqI TCGA 1 cut(s) 122
TatI WGTACW 2 cut(s) 192, 459
TauI GCSGC 5 cut(s) 80, 239, 266, 278, 324
Tru1I TTAA 1 cut(s) 72
Tru9I TTAA 1 cut(s) 72
TscAI CASTG 1 cut(s) 331
TseFI GTSAC 1 cut(s) 229
TseI GCWGC 1 cut(s) 470
Tsp45I GTSAC 1 cut(s) 229
TspDTI ATGAA 2 cut(s) 247, 402
TspGWI ACGGA 1 cut(s) 441
TspRI CASTG 1 cut(s) 331
VpaK11BI GGWCC 1 cut(s) 404
XbaI TCTAGA 1 cut(s) 527
XcmI CCANNNNNNNNNTGG 1 cut(s) 109
XhoI CTCGAG 1 cut(s) 121
XspI CTAG 1 cut(s) 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.