pycom16g02740

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0003619
Physical Location & Seq
Reverse (-)
1764580 .. 1765380
801 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g02740.1

Sequence Viewer

Length: 747 bp
ATGAAACAACCACTAAAATTCAAGGAGCAAATTTTAGAACCAGTTCCACTTGCGACTATAAAACCCCCTCACAACGTTTTCTCACAAACCAACCAACTCTTTAACCAGATTCCAATTCATCGAAGTTTACATAGGATTCTATCTCTCTCTCTCTCTCTCTCTCTAGACTCTCAAATGGGAAACTACATCTCATGCAATTTGGCCACGCCGATGATCAAGAGCTCCAAATCAGCGAGGGTGATCTTCCCCAACGGCGACGTCCGGCAGTTCCGGGAGACGACCAAGGCGGCGGAGCTCATGCTGGAGTGTCCCAACGCCTTCTTGGCCAACTCGACTTCTCTCCACATGGGCCGGAGATTCTCCGCCCTCAGCGCCGATGAGGAGTTGGAGTTCGGGAACATATACATCATGTTTCCAATGAGGAGGCTCAGCTCCATAGTCACGGCGGCTGACATGGCCGTGTTCTTCATGGCGGCTAACTCCGCTACCAAGCGTATATCCGGCTGGAAGGTTAGGATATTGCCGGAGAGTGGTGGCGGAGGAGGAGAAGTGGTGGCGGTGGAGAGTGGAAAAGAAAATCATGGTGATCAAGATCATACTAGAAGGGATCATCAAGGTCCATTGCGGCTGAGCTTGGACGGAGTTGAGGGGTTGCAAGTGGCAGAGTTTCAGTACAGATTAGCTGCGTCTAGGTCAAGGAAGCCGGTGTTGGAAACCATCAAAGAAGAGCCGATTGGTTCCAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

249

Amino Acids

27.56

Weight (kDa)

9.32

Isoelectric Point (pI)

48.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 59 - 242 4.4e-36 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 261
AciI CCGC 9 cut(s) 287, 290, 363, 446, 473, 483, 537, 557, 625
AclI AACGTT 1 cut(s) 75
AclWI GGATC 1 cut(s) 615
AcoI YGGCCR 3 cut(s) 201, 324, 456
AcsI RAATTY 2 cut(s) 17, 30
AcyI GRCGYC 1 cut(s) 258
AfaI GTAC 1 cut(s) 674
AfiI CCNNNNNNNGG 1 cut(s) 530
AgsI TTSAA 1 cut(s) 22
AjuI GAANNNNNNNTTGG 3 cut(s) 692, 717, 724
AloI GAACNNNNNNTCC 2 cut(s) 374, 406
AluBI AGCT 5 cut(s) 222, 295, 432, 633, 683
AluI AGCT 5 cut(s) 222, 295, 432, 633, 683
Alw21I GWGCWC 2 cut(s) 224, 297
Alw26I GTCTC 1 cut(s) 269
AlwI GGATC 1 cut(s) 615
AoxI GGCC 4 cut(s) 201, 324, 349, 456
ApeKI GCWGC 1 cut(s) 683
ApoI RAATTY 2 cut(s) 17, 30
Asp700I GAANNNNTTC 1 cut(s) 42
AspLEI GCGC 1 cut(s) 374
AspS9I GGNCC 2 cut(s) 349, 617
AsuC2I CCSGG 1 cut(s) 272
AsuHPI GGTGA 2 cut(s) 250, 596
AvaII GGWCC 1 cut(s) 617
BalI TGGCCA 2 cut(s) 203, 326
BanII GRGCYC 2 cut(s) 224, 297
Bbv12I GWGCWC 2 cut(s) 224, 297
BbvCI CCTCAGC 1 cut(s) 368
BbvI GCAGC 1 cut(s) 670
BccI CCATC 1 cut(s) 725
BceAI ACGGC 3 cut(s) 268, 443, 459
BclI TGATCA 2 cut(s) 213, 586
BcnI CCSGG 1 cut(s) 272
BcoDI GTCTC 1 cut(s) 269
BfaI CTAG 3 cut(s) 164, 600, 690
BfoI RGCGCY 1 cut(s) 375
BglI GCCNNNNNGGC 1 cut(s) 323
BisI GCNGC 5 cut(s) 288, 447, 474, 626, 684
BlpI GCTNAGC 2 cut(s) 428, 629
BlsI GCNGC 5 cut(s) 289, 448, 475, 627, 685
Bme1390I CCNGG 1 cut(s) 272
Bme18I GGWCC 1 cut(s) 617
BmgT120I GGNCC 2 cut(s) 349, 617
BmiI GGNNCC 1 cut(s) 739
BmrFI CCNGG 1 cut(s) 272
BpmI CTGGAG 1 cut(s) 323
Bpu10I CCTNAGC 1 cut(s) 368
Bpu1102I GCTNAGC 2 cut(s) 428, 629
BpuMI CCSGG 1 cut(s) 272
BsaBI GATNNNNATC 1 cut(s) 591
BsaHI GRCGYC 1 cut(s) 258
BsaJI CCNNGG 1 cut(s) 282
BsaXI ACNNNNNCTCC 6 cut(s) 374, 404, 534, 537, 564, 567
Bsc4I CCNNNNNNNGG 1 cut(s) 530
Bse118I RCCGGY 1 cut(s) 703
Bse1I ACTGG 1 cut(s) 41
Bse3DI GCAATG 1 cut(s) 620
Bse8I GATNNNNATC 1 cut(s) 591
BseDI CCNNGG 1 cut(s) 282
BseJI GATNNNNATC 1 cut(s) 591
BseLI CCNNNNNNNGG 1 cut(s) 530
BseMI GCAATG 1 cut(s) 620
BseMII CTCAG 3 cut(s) 382, 442, 620
BseNI ACTGG 1 cut(s) 41
BseRI GAGGAG 4 cut(s) 395, 436, 555, 558
BseXI GCAGC 1 cut(s) 670
BshFI GGCC 4 cut(s) 203, 326, 351, 458
BsiHKAI GWGCWC 2 cut(s) 224, 297
BsiSI CCGG 6 cut(s) 262, 271, 352, 501, 524, 704
BslFI GGGAC 1 cut(s) 294
BslI CCNNNNNNNGG 1 cut(s) 530
BsmAI GTCTC 1 cut(s) 269
BsmBI CGTCTC 1 cut(s) 269
BsmFI GGGAC 1 cut(s) 294
BsnI GGCC 4 cut(s) 203, 326, 351, 458
Bsp1286I GDGCHC 2 cut(s) 224, 297
Bsp143I GATC 5 cut(s) 213, 240, 586, 592, 607
Bsp1720I GCTNAGC 2 cut(s) 428, 629
BspACI CCGC 9 cut(s) 287, 290, 363, 446, 473, 483, 537, 557, 625
BspANI GGCC 4 cut(s) 203, 326, 351, 458
BspCNI CTCAG 3 cut(s) 381, 441, 621
BspLI GGNNCC 1 cut(s) 739
BspPI GGATC 1 cut(s) 615
BspQI GCTCTTC 1 cut(s) 720
BsrDI GCAATG 1 cut(s) 620
BsrFI RCCGGY 1 cut(s) 703
BsrI ACTGG 1 cut(s) 41
BssAI RCCGGY 1 cut(s) 703
BssECI CCNNGG 1 cut(s) 282
BssMI GATC 5 cut(s) 213, 240, 586, 592, 607
BssNI GRCGYC 1 cut(s) 258
BssT1I CCWWGG 1 cut(s) 282
Bst6I CTCTTC 1 cut(s) 720
BstACI GRCGYC 1 cut(s) 258
BstDEI CTNAG 3 cut(s) 368, 428, 629
BstH2I RGCGCY 1 cut(s) 375
BstHHI GCGC 1 cut(s) 374
BstKTI GATC 5 cut(s) 216, 243, 589, 595, 610
BstMAI GTCTC 1 cut(s) 269
BstMBI GATC 5 cut(s) 213, 240, 586, 592, 607
BstMWI GCNNNNNNNGC 4 cut(s) 323, 371, 455, 482
BstSCI CCNGG 1 cut(s) 270
BstV1I GCAGC 1 cut(s) 670
BsuRI GGCC 4 cut(s) 203, 326, 351, 458
CfoI GCGC 1 cut(s) 374
Cfr10I RCCGGY 1 cut(s) 703
Cfr13I GGNCC 2 cut(s) 349, 617
CseI GACGC 1 cut(s) 675
Csp6I GTAC 1 cut(s) 673
CviAII CATG 7 cut(s) 192, 298, 346, 409, 454, 469, 581
CviQI GTAC 1 cut(s) 673
DdeI CTNAG 3 cut(s) 368, 428, 629
DpnI GATC 5 cut(s) 215, 242, 588, 594, 609
DpnII GATC 5 cut(s) 213, 240, 586, 592, 607
EaeI YGGCCR 3 cut(s) 201, 324, 456
Eam1104I CTCTTC 1 cut(s) 720
EarI CTCTTC 1 cut(s) 720
EciI GGCGGA 3 cut(s) 305, 352, 552
Ecl136II GAGCTC 2 cut(s) 222, 295
Eco130I CCWWGG 1 cut(s) 282
Eco24I GRGCYC 2 cut(s) 224, 297
Eco47I GGWCC 1 cut(s) 617
Eco53kI GAGCTC 2 cut(s) 222, 295
EcoICRI GAGCTC 2 cut(s) 222, 295
EcoT14I CCWWGG 1 cut(s) 282
EcoT38I GRGCYC 2 cut(s) 224, 297
ErhI CCWWGG 1 cut(s) 282
Esp3I CGTCTC 1 cut(s) 269
FaeI CATG 7 cut(s) 195, 301, 349, 412, 457, 472, 584
FaqI GGGAC 1 cut(s) 294
FatI CATG 7 cut(s) 191, 297, 345, 408, 453, 468, 580
FbaI TGATCA 2 cut(s) 213, 586
Fnu4HI GCNGC 5 cut(s) 288, 447, 474, 626, 684
FriOI GRGCYC 2 cut(s) 224, 297
Fsp4HI GCNGC 5 cut(s) 288, 447, 474, 626, 684
FspBI CTAG 3 cut(s) 164, 600, 690
GlaI GCGC 1 cut(s) 373
GluI GCNGC 5 cut(s) 288, 447, 474, 626, 684
GsuI CTGGAG 1 cut(s) 323
HaeII RGCGCY 1 cut(s) 375
HaeIII GGCC 4 cut(s) 203, 326, 351, 458
HapII CCGG 6 cut(s) 262, 271, 352, 501, 524, 704
HgaI GACGC 1 cut(s) 675
HhaI GCGC 1 cut(s) 374
Hin1I GRCGYC 1 cut(s) 258
Hin1II CATG 7 cut(s) 195, 301, 349, 412, 457, 472, 584
Hin6I GCGC 1 cut(s) 372
HinP1I GCGC 1 cut(s) 372
HinfI GANTC 4 cut(s) 109, 136, 167, 357
HpaII CCGG 6 cut(s) 262, 271, 352, 501, 524, 704
HphI GGTGA 2 cut(s) 250, 596
Hpy166II GTNNAC 1 cut(s) 128
Hpy188III TCNNGA 5 cut(s) 164, 217, 394, 590, 741
Hpy8I GTNNAC 1 cut(s) 128
Hpy99I CGWCG 1 cut(s) 260
HpyAV CCTTC 3 cut(s) 328, 502, 597
HpyCH4IV ACGT 2 cut(s) 75, 258
HpyCH4V TGCA 2 cut(s) 195, 655
HpyF10VI GCNNNNNNNGC 4 cut(s) 323, 371, 455, 482
HpyF3I CTNAG 3 cut(s) 368, 428, 629
HpySE526I ACGT 2 cut(s) 75, 258
Hsp92I GRCGYC 1 cut(s) 258
Hsp92II CATG 7 cut(s) 195, 301, 349, 412, 457, 472, 584
HspAI GCGC 1 cut(s) 372
Ksp22I TGATCA 2 cut(s) 213, 586
Kzo9I GATC 5 cut(s) 213, 240, 586, 592, 607
LguI GCTCTTC 1 cut(s) 720
LmnI GCTCC 4 cut(s) 25, 227, 292, 437
Lsp1109I GCAGC 1 cut(s) 670
MaeI CTAG 3 cut(s) 164, 600, 690
MaeII ACGT 2 cut(s) 75, 258
MaeIII GTNAC 1 cut(s) 439
MalI GATC 5 cut(s) 215, 242, 588, 594, 609
MboI GATC 5 cut(s) 213, 240, 586, 592, 607
MboII GAAGA 3 cut(s) 235, 457, 737
MhlI GDGCHC 2 cut(s) 224, 297
MlsI TGGCCA 2 cut(s) 203, 326
MluCI AATT 4 cut(s) 17, 30, 114, 196
MluNI TGGCCA 2 cut(s) 203, 326
MlyI GAGTC 1 cut(s) 161
MmeI TCCRAC 2 cut(s) 366, 690
MnlI CCTC 9 cut(s) 78, 228, 373, 377, 414, 417, 533, 536, 640
Mox20I TGGCCA 2 cut(s) 203, 326
MroXI GAANNNNTTC 1 cut(s) 42
MscI TGGCCA 2 cut(s) 203, 326
MseI TTAA 1 cut(s) 102
MslI CAYNNNNRTG 2 cut(s) 209, 458
Msp20I TGGCCA 2 cut(s) 203, 326
MspI CCGG 6 cut(s) 262, 271, 352, 501, 524, 704
MspR9I CCNGG 1 cut(s) 272
MwoI GCNNNNNNNGC 4 cut(s) 323, 371, 455, 482
NciI CCSGG 1 cut(s) 272
NdeII GATC 5 cut(s) 213, 240, 586, 592, 607
NlaIII CATG 7 cut(s) 195, 301, 349, 412, 457, 472, 584
NlaIV GGNNCC 1 cut(s) 739
NmuCI GTSAC 1 cut(s) 439
PciSI GCTCTTC 1 cut(s) 720
PdmI GAANNNNTTC 1 cut(s) 42
PfeI GAWTC 3 cut(s) 109, 136, 357
PfoI TCCNGGA 1 cut(s) 270
PkrI GCNGC 5 cut(s) 289, 448, 475, 627, 685
PleI GAGTC 1 cut(s) 161
PpsI GAGTC 1 cut(s) 161
Psp124BI GAGCTC 2 cut(s) 224, 297
Psp1406I AACGTT 1 cut(s) 75
PspN4I GGNNCC 1 cut(s) 739
PspPI GGNCC 2 cut(s) 349, 617
RsaI GTAC 1 cut(s) 674
RsaNI GTAC 1 cut(s) 673
RseI CAYNNNNRTG 2 cut(s) 209, 458
SacI GAGCTC 2 cut(s) 224, 297
SapI GCTCTTC 1 cut(s) 720
SaqAI TTAA 1 cut(s) 102
SatI GCNGC 5 cut(s) 288, 447, 474, 626, 684
Sau3AI GATC 5 cut(s) 213, 240, 586, 592, 607
Sau96I GGNCC 2 cut(s) 349, 617
SchI GAGTC 1 cut(s) 161
ScrFI CCNGG 1 cut(s) 272
SduI GDGCHC 2 cut(s) 224, 297
SinI GGWCC 1 cut(s) 617
SmiMI CAYNNNNRTG 2 cut(s) 209, 458
Sse9I AATT 4 cut(s) 17, 30, 114, 196
SsiI CCGC 9 cut(s) 287, 290, 363, 446, 473, 483, 537, 557, 625
SspMI CTAG 3 cut(s) 164, 600, 690
SstI GAGCTC 2 cut(s) 224, 297
StyD4I CCNGG 1 cut(s) 270
StyI CCWWGG 1 cut(s) 282
TaiI ACGT 2 cut(s) 78, 261
TaqI TCGA 2 cut(s) 121, 332
TasI AATT 4 cut(s) 17, 30, 114, 196
TatI WGTACW 1 cut(s) 672
TauI GCSGC 4 cut(s) 290, 449, 476, 628
TfiI GAWTC 3 cut(s) 109, 136, 357
Tru1I TTAA 1 cut(s) 102
Tru9I TTAA 1 cut(s) 102
TseFI GTSAC 1 cut(s) 439
TseI GCWGC 1 cut(s) 683
Tsp45I GTSAC 1 cut(s) 439
TspDTI ATGAA 3 cut(s) 17, 107, 457
TspGWI ACGGA 1 cut(s) 654
VpaK11BI GGWCC 1 cut(s) 617
XapI RAATTY 2 cut(s) 17, 30
XbaI TCTAGA 1 cut(s) 163
XcmI CCANNNNNNNNNTGG 1 cut(s) 319
XmnI GAANNNNTTC 1 cut(s) 42
XspI CTAG 3 cut(s) 164, 600, 690
ZraI GACGTC 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.