Rh3AG312400

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0003619
Physical Location & Seq
Forward (+)
39332011 .. 39332772
762 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG312400.1

Sequence Viewer

Length: 438 bp
ATGGGCAGGAGGTTTTCTGCTCTTACTGCAGACCAAGACTTGGAGATGGGCAGCGTTTACGTCATGTTTCCCATGAAGAGGCTCAACTCTACGGTCACATCTTCTGACATGGCCGCCTTGTTCCTCACCGCCGTTGCCACTCCCAAGGCCATGCACAAGCCCAAACGGCAGCTTTCCGGTGGCAAGGTCAGGGTGCGGCCGGCTGATCATGACGATCACGATCAAACCACCATTAATAAGATGTATTCAGACAAGGGGCCAAAGCTAAATTTGGATGATATCGAAGAGTTCTCGGCCCCAGAGTTCATGCATCGATTATCCATGAGTAGGTCCAAGAAGCCATTGTTGGAGACCATAACAGAAGAGGCTGTTAGTGTTTGTTCAAGGAAAATAATCCTCGAAGGCCTCTGGCCCAGAGTCCCAGACCCGGCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

145

Amino Acids

16.23

Weight (kDa)

8.73

Isoelectric Point (pI)

60.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 2 - 121 5.3e-16 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 40
AciI CCGC 3 cut(s) 114, 129, 196
AcoI YGGCCR 2 cut(s) 111, 197
AcsI RAATTY 1 cut(s) 268
AfiI CCNNNNNNNGG 4 cut(s) 40, 78, 327, 427
AgsI TTSAA 1 cut(s) 384
AjuI GAANNNNNNNTTGG 2 cut(s) 329, 361
AluBI AGCT 2 cut(s) 172, 265
AluI AGCT 2 cut(s) 172, 265
Alw26I GTCTC 1 cut(s) 344
AoxI GGCC 7 cut(s) 111, 147, 197, 257, 294, 403, 410
ApeKI GCWGC 3 cut(s) 51, 169, 431
ApoI RAATTY 1 cut(s) 268
AseI ATTAAT 1 cut(s) 234
AspS9I GGNCC 4 cut(s) 257, 295, 330, 411
AsuC2I CCSGG 1 cut(s) 428
AsuHPI GGTGA 1 cut(s) 118
AvaII GGWCC 1 cut(s) 330
BbvI GCAGC 3 cut(s) 63, 181, 418
BccI CCATC 1 cut(s) 40
BceAI ACGGC 2 cut(s) 116, 182
BclI TGATCA 1 cut(s) 205
BcnI CCSGG 1 cut(s) 428
BcoDI GTCTC 1 cut(s) 344
BfmI CTRYAG 1 cut(s) 27
BglI GCCNNNNNGGC 1 cut(s) 166
BisI GCNGC 5 cut(s) 52, 114, 170, 197, 432
BlsI GCNGC 5 cut(s) 53, 115, 171, 198, 433
Bme1390I CCNGG 1 cut(s) 428
Bme18I GGWCC 1 cut(s) 330
BmgT120I GGNCC 4 cut(s) 257, 295, 330, 411
BmiI GGNNCC 2 cut(s) 258, 297
BmrFI CCNGG 1 cut(s) 428
BmsI GCATC 1 cut(s) 319
BpuMI CCSGG 1 cut(s) 428
Bsa29I ATCGAT 1 cut(s) 313
BsaBI GATNNNNATC 1 cut(s) 219
BsaI GGTCTC 1 cut(s) 344
BsaJI CCNNGG 1 cut(s) 144
BsaWI WCCGGW 1 cut(s) 176
Bsc4I CCNNNNNNNGG 4 cut(s) 40, 78, 327, 427
Bse118I RCCGGY 1 cut(s) 199
Bse8I GATNNNNATC 1 cut(s) 219
BseCI ATCGAT 1 cut(s) 313
BseDI CCNNGG 1 cut(s) 144
BseGI GGATG 1 cut(s) 280
BseJI GATNNNNATC 1 cut(s) 219
BseLI CCNNNNNNNGG 4 cut(s) 40, 78, 327, 427
BseX3I CGGCCG 1 cut(s) 197
BseXI GCAGC 3 cut(s) 63, 181, 418
Bsh1285I CGRYCG 1 cut(s) 200
BshFI GGCC 7 cut(s) 113, 149, 199, 259, 296, 405, 412
BshVI ATCGAT 1 cut(s) 313
BsiEI CGRYCG 1 cut(s) 200
BsiSI CCGG 3 cut(s) 177, 200, 428
BslFI GGGAC 1 cut(s) 404
BslI CCNNNNNNNGG 4 cut(s) 40, 78, 327, 427
BsmAI GTCTC 1 cut(s) 344
BsmFI GGGAC 1 cut(s) 404
BsnI GGCC 7 cut(s) 113, 149, 199, 259, 296, 405, 412
Bso31I GGTCTC 1 cut(s) 344
Bsp143I GATC 3 cut(s) 205, 214, 220
BspACI CCGC 3 cut(s) 114, 129, 196
BspANI GGCC 7 cut(s) 113, 149, 199, 259, 296, 405, 412
BspDI ATCGAT 1 cut(s) 313
BspHI TCATGA 1 cut(s) 208
BspLI GGNNCC 2 cut(s) 258, 297
BspMAI CTGCAG 1 cut(s) 31
BspTNI GGTCTC 1 cut(s) 344
BsrFI RCCGGY 1 cut(s) 199
BssAI RCCGGY 1 cut(s) 199
BssECI CCNNGG 1 cut(s) 144
BssMI GATC 3 cut(s) 205, 214, 220
BssT1I CCWWGG 1 cut(s) 144
Bst4CI ACNGT 1 cut(s) 94
Bst6I CTCTTC 3 cut(s) 71, 279, 357
BstC8I GCNNGC 1 cut(s) 201
BstF5I GGATG 1 cut(s) 280
BstKTI GATC 3 cut(s) 208, 217, 223
BstMAI GTCTC 1 cut(s) 344
BstMBI GATC 3 cut(s) 205, 214, 220
BstMCI CGRYCG 1 cut(s) 200
BstMWI GCNNNNNNNGC 2 cut(s) 26, 166
BstSCI CCNGG 1 cut(s) 426
BstSFI CTRYAG 1 cut(s) 27
BstV1I GCAGC 3 cut(s) 63, 181, 418
BstZI CGGCCG 1 cut(s) 197
Bsu15I ATCGAT 1 cut(s) 313
BsuRI GGCC 7 cut(s) 113, 149, 199, 259, 296, 405, 412
BsuTUI ATCGAT 1 cut(s) 313
BtsCI GGATG 1 cut(s) 280
Cac8I GCNNGC 1 cut(s) 201
CciI TCATGA 1 cut(s) 208
Cfr10I RCCGGY 1 cut(s) 199
Cfr13I GGNCC 4 cut(s) 257, 295, 330, 411
ClaI ATCGAT 1 cut(s) 313
CviAII CATG 7 cut(s) 64, 73, 109, 151, 209, 307, 322
DpnI GATC 3 cut(s) 207, 216, 222
DpnII GATC 3 cut(s) 205, 214, 220
EaeI YGGCCR 2 cut(s) 111, 197
EagI CGGCCG 1 cut(s) 197
Eam1104I CTCTTC 3 cut(s) 71, 279, 357
EarI CTCTTC 3 cut(s) 71, 279, 357
EclXI CGGCCG 1 cut(s) 197
Eco130I CCWWGG 1 cut(s) 144
Eco147I AGGCCT 1 cut(s) 405
Eco31I GGTCTC 1 cut(s) 344
Eco32I GATATC 1 cut(s) 280
Eco47I GGWCC 1 cut(s) 330
Eco52I CGGCCG 1 cut(s) 197
EcoRV GATATC 1 cut(s) 280
EcoT14I CCWWGG 1 cut(s) 144
EcoT22I ATGCAT 1 cut(s) 312
ErhI CCWWGG 1 cut(s) 144
FaeI CATG 7 cut(s) 67, 76, 112, 154, 212, 310, 325
FaiI YATR 8 cut(s) 65, 74, 110, 152, 210, 308, 323, 356
FaqI GGGAC 1 cut(s) 404
FatI CATG 7 cut(s) 63, 72, 108, 150, 208, 306, 321
FbaI TGATCA 1 cut(s) 205
Fnu4HI GCNGC 5 cut(s) 52, 114, 170, 197, 432
FokI GGATG 1 cut(s) 287
Fsp4HI GCNGC 5 cut(s) 52, 114, 170, 197, 432
GluI GCNGC 5 cut(s) 52, 114, 170, 197, 432
HaeIII GGCC 7 cut(s) 113, 149, 199, 259, 296, 405, 412
HapII CCGG 3 cut(s) 177, 200, 428
Hin1II CATG 7 cut(s) 67, 76, 112, 154, 212, 310, 325
HinfI GANTC 1 cut(s) 417
HpaII CCGG 3 cut(s) 177, 200, 428
HphI GGTGA 1 cut(s) 118
Hpy166II GTNNAC 1 cut(s) 58
Hpy188I TCNGA 2 cut(s) 106, 250
Hpy188III TCNNGA 2 cut(s) 209, 218
Hpy8I GTNNAC 1 cut(s) 58
HpyAV CCTTC 1 cut(s) 395
HpyCH4III ACNGT 1 cut(s) 94
HpyCH4IV ACGT 1 cut(s) 60
HpyCH4V TGCA 3 cut(s) 29, 154, 310
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 166
HpySE526I ACGT 1 cut(s) 60
Hsp92II CATG 7 cut(s) 67, 76, 112, 154, 212, 310, 325
KroI GCCGGC 1 cut(s) 199
KroNI GCCGGC 1 cut(s) 201
Ksp22I TGATCA 1 cut(s) 205
Kzo9I GATC 3 cut(s) 205, 214, 220
LpnPI CCDG 6 cut(s) 175, 190, 213, 312, 394, 427
Lsp1109I GCAGC 3 cut(s) 63, 181, 418
LweI GCATC 1 cut(s) 319
MaeII ACGT 1 cut(s) 60
MaeIII GTNAC 1 cut(s) 94
MalI GATC 3 cut(s) 207, 216, 222
MboI GATC 3 cut(s) 205, 214, 220
MboII GAAGA 4 cut(s) 88, 93, 296, 374
MluCI AATT 1 cut(s) 268
MlyI GAGTC 1 cut(s) 426
MmeI TCCRAC 1 cut(s) 327
MnlI CCTC 6 cut(s) 3, 72, 134, 358, 407, 416
Mph1103I ATGCAT 1 cut(s) 312
MroNI GCCGGC 1 cut(s) 199
MseI TTAA 1 cut(s) 234
MspI CCGG 3 cut(s) 177, 200, 428
MspR9I CCNGG 1 cut(s) 428
MwoI GCNNNNNNNGC 2 cut(s) 26, 166
NaeI GCCGGC 1 cut(s) 201
NciI CCSGG 1 cut(s) 428
NdeII GATC 3 cut(s) 205, 214, 220
NgoMIV GCCGGC 1 cut(s) 199
NlaIII CATG 7 cut(s) 67, 76, 112, 154, 212, 310, 325
NlaIV GGNNCC 2 cut(s) 258, 297
NmeAIII GCCGAG 1 cut(s) 272
NmuCI GTSAC 1 cut(s) 94
NsiI ATGCAT 1 cut(s) 312
PagI TCATGA 1 cut(s) 208
PceI AGGCCT 1 cut(s) 405
PdiI GCCGGC 1 cut(s) 201
PflMI CCANNNNNTGG 1 cut(s) 40
PkrI GCNGC 5 cut(s) 53, 115, 171, 198, 433
PleI GAGTC 1 cut(s) 425
PpsI GAGTC 1 cut(s) 425
PshBI ATTAAT 1 cut(s) 234
PspN4I GGNNCC 2 cut(s) 258, 297
PspPI GGNCC 4 cut(s) 257, 295, 330, 411
PstI CTGCAG 1 cut(s) 31
SaqAI TTAA 1 cut(s) 234
SatI GCNGC 5 cut(s) 52, 114, 170, 197, 432
Sau3AI GATC 3 cut(s) 205, 214, 220
Sau96I GGNCC 4 cut(s) 257, 295, 330, 411
SchI GAGTC 1 cut(s) 426
ScrFI CCNGG 1 cut(s) 428
SetI ASST 6 cut(s) 14, 63, 174, 189, 267, 332
SfaNI GCATC 1 cut(s) 319
SfcI CTRYAG 1 cut(s) 27
SinI GGWCC 1 cut(s) 330
Sse9I AATT 1 cut(s) 268
SseBI AGGCCT 1 cut(s) 405
SsiI CCGC 3 cut(s) 114, 129, 196
StuI AGGCCT 1 cut(s) 405
StyD4I CCNGG 1 cut(s) 426
StyI CCWWGG 1 cut(s) 144
TaaI ACNGT 1 cut(s) 94
TaiI ACGT 1 cut(s) 63
TaqI TCGA 3 cut(s) 282, 313, 399
TasI AATT 1 cut(s) 268
TauI GCSGC 2 cut(s) 116, 199
Tru1I TTAA 1 cut(s) 234
Tru9I TTAA 1 cut(s) 234
TseFI GTSAC 1 cut(s) 94
TseI GCWGC 3 cut(s) 51, 169, 431
Tsp45I GTSAC 1 cut(s) 94
TspDTI ATGAA 2 cut(s) 89, 295
Van91I CCANNNNNTGG 1 cut(s) 40
VpaK11BI GGWCC 1 cut(s) 330
VspI ATTAAT 1 cut(s) 234
XapI RAATTY 1 cut(s) 268
Zsp2I ATGCAT 1 cut(s) 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.