Rh3BG348200

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0003619
Physical Location & Seq
Forward (+)
38270483 .. 38271327
845 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG348200.1

Sequence Viewer

Length: 504 bp
ATGAATGAACCGGTAAAAGCCGCGGAGGTGATGCTCGACACCCCAAACTACTTCCTGGTAAACTCCAAGTATCTCCATATGGGCAGGAGGTTTTCTGCTCTTACTGCAGACCAAGACTTGGAGATGGGCAGCGTTTACGTCATGTTTCCCATGAAGAGGCTCAACTCTACAGTCACATCTTCTGACATGGCCGCCTTGTTCCTCACCGCCGTTGCCGCTCCCAGGGCCATGCACAAGCCCAAACGGCAGCTTTCCGGTGGCAAGGTCAGGGTGCGGCCGGCTGATCATGACGATCACAATCAAACCACCATTAATAAGATGAATTCAGACAAGGGGCCAAAGCTAAATTTGGATGATATCGAAGAGTTCTCGGCCCCAGAGTTCATGCATCGATTATCCATGAGTAGGTCCAAGAAGCCATTGTTGGAGACCATAACAGAAGAGGCTGAAAATAATCCTGGAAGGCCTCTGGCCCAGACCCGGCTGCTTGAAAGGTTTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

167

Amino Acids

18.92

Weight (kDa)

8.01

Isoelectric Point (pI)

51.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 2 - 147 3e-27 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 118
AccBSI CCGCTC 1 cut(s) 218
AccII CGCG 1 cut(s) 23
AciI CCGC 6 cut(s) 21, 23, 192, 207, 216, 274
AcoI YGGCCR 2 cut(s) 189, 275
AcsI RAATTY 2 cut(s) 322, 346
AfiI CCNNNNNNNGG 5 cut(s) 118, 156, 222, 405, 480
AgeI ACCGGT 1 cut(s) 10
AgsI TTSAA 2 cut(s) 491, 500
AjnI CCWGG 3 cut(s) 54, 221, 457
AjuI GAANNNNNNNTTGG 2 cut(s) 407, 439
AluBI AGCT 2 cut(s) 250, 343
AluI AGCT 2 cut(s) 250, 343
Alw26I GTCTC 1 cut(s) 422
AoxI GGCC 7 cut(s) 189, 225, 275, 335, 372, 464, 471
ApeKI GCWGC 3 cut(s) 129, 247, 484
ApoI RAATTY 2 cut(s) 322, 346
AseI ATTAAT 1 cut(s) 312
AsiGI ACCGGT 1 cut(s) 10
AspS9I GGNCC 5 cut(s) 225, 335, 373, 408, 472
AsuC2I CCSGG 1 cut(s) 481
AsuHPI GGTGA 2 cut(s) 40, 196
AvaII GGWCC 1 cut(s) 408
BbvI GCAGC 3 cut(s) 141, 259, 471
BccI CCATC 1 cut(s) 118
BceAI ACGGC 2 cut(s) 194, 260
BciT130I CCWGG 3 cut(s) 56, 223, 459
BclI TGATCA 1 cut(s) 283
BcnI CCSGG 1 cut(s) 481
BcoDI GTCTC 1 cut(s) 422
BfmI CTRYAG 2 cut(s) 105, 168
BglI GCCNNNNNGGC 1 cut(s) 244
BisI GCNGC 7 cut(s) 21, 130, 192, 216, 248, 275, 485
BlsI GCNGC 7 cut(s) 22, 131, 193, 217, 249, 276, 486
Bme1390I CCNGG 4 cut(s) 56, 223, 459, 481
Bme18I GGWCC 1 cut(s) 408
BmgT120I GGNCC 5 cut(s) 225, 335, 373, 408, 472
BmiI GGNNCC 2 cut(s) 336, 375
BmrFI CCNGG 4 cut(s) 56, 223, 459, 481
BmsI GCATC 2 cut(s) 21, 397
BpuMI CCSGG 1 cut(s) 481
Bsa29I ATCGAT 1 cut(s) 391
BsaBI GATNNNNATC 1 cut(s) 297
BsaI GGTCTC 1 cut(s) 422
BsaJI CCNNGG 3 cut(s) 21, 221, 222
BsaWI WCCGGW 2 cut(s) 10, 254
Bsc4I CCNNNNNNNGG 5 cut(s) 118, 156, 222, 405, 480
Bse118I RCCGGY 2 cut(s) 10, 277
Bse8I GATNNNNATC 1 cut(s) 297
BseBI CCWGG 3 cut(s) 56, 223, 459
BseCI ATCGAT 1 cut(s) 391
BseDI CCNNGG 3 cut(s) 21, 221, 222
BseGI GGATG 1 cut(s) 358
BseJI GATNNNNATC 1 cut(s) 297
BseLI CCNNNNNNNGG 5 cut(s) 118, 156, 222, 405, 480
BseX3I CGGCCG 1 cut(s) 275
BseXI GCAGC 3 cut(s) 141, 259, 471
Bsh1236I CGCG 1 cut(s) 23
Bsh1285I CGRYCG 1 cut(s) 278
BshFI GGCC 7 cut(s) 191, 227, 277, 337, 374, 466, 473
BshTI ACCGGT 1 cut(s) 10
BshVI ATCGAT 1 cut(s) 391
BsiEI CGRYCG 1 cut(s) 278
BsiSI CCGG 4 cut(s) 11, 255, 278, 481
BslI CCNNNNNNNGG 5 cut(s) 118, 156, 222, 405, 480
BsmAI GTCTC 1 cut(s) 422
BsnI GGCC 7 cut(s) 191, 227, 277, 337, 374, 466, 473
Bso31I GGTCTC 1 cut(s) 422
Bsp143I GATC 2 cut(s) 283, 292
BspACI CCGC 6 cut(s) 21, 23, 192, 207, 216, 274
BspANI GGCC 7 cut(s) 191, 227, 277, 337, 374, 466, 473
BspDI ATCGAT 1 cut(s) 391
BspFNI CGCG 1 cut(s) 23
BspHI TCATGA 1 cut(s) 286
BspLI GGNNCC 2 cut(s) 336, 375
BspMAI CTGCAG 1 cut(s) 109
BspTNI GGTCTC 1 cut(s) 422
BsrBI CCGCTC 1 cut(s) 218
BsrFI RCCGGY 2 cut(s) 10, 277
BssAI RCCGGY 2 cut(s) 10, 277
BssECI CCNNGG 3 cut(s) 21, 221, 222
BssMI GATC 2 cut(s) 283, 292
Bst2UI CCWGG 3 cut(s) 56, 223, 459
Bst4CI ACNGT 1 cut(s) 172
Bst6I CTCTTC 3 cut(s) 149, 357, 435
BstC8I GCNNGC 1 cut(s) 279
BstDSI CCRYGG 1 cut(s) 21
BstF5I GGATG 1 cut(s) 358
BstFNI CGCG 1 cut(s) 23
BstKTI GATC 2 cut(s) 286, 295
BstMAI GTCTC 1 cut(s) 422
BstMBI GATC 2 cut(s) 283, 292
BstMCI CGRYCG 1 cut(s) 278
BstMWI GCNNNNNNNGC 4 cut(s) 104, 215, 224, 244
BstNI CCWGG 3 cut(s) 56, 223, 459
BstSCI CCNGG 4 cut(s) 54, 221, 457, 479
BstSFI CTRYAG 2 cut(s) 105, 168
BstUI CGCG 1 cut(s) 23
BstV1I GCAGC 3 cut(s) 141, 259, 471
BstZI CGGCCG 1 cut(s) 275
Bsu15I ATCGAT 1 cut(s) 391
BsuRI GGCC 7 cut(s) 191, 227, 277, 337, 374, 466, 473
BsuTUI ATCGAT 1 cut(s) 391
BtgI CCRYGG 1 cut(s) 21
BtsCI GGATG 1 cut(s) 358
Cac8I GCNNGC 1 cut(s) 279
CciI TCATGA 1 cut(s) 286
Cfr10I RCCGGY 2 cut(s) 10, 277
Cfr13I GGNCC 5 cut(s) 225, 335, 373, 408, 472
Cfr42I CCGCGG 1 cut(s) 24
ClaI ATCGAT 1 cut(s) 391
CspAI ACCGGT 1 cut(s) 10
CviAII CATG 7 cut(s) 142, 151, 187, 229, 287, 385, 400
DpnI GATC 2 cut(s) 285, 294
DpnII GATC 2 cut(s) 283, 292
EaeI YGGCCR 2 cut(s) 189, 275
EagI CGGCCG 1 cut(s) 275
Eam1104I CTCTTC 3 cut(s) 149, 357, 435
EarI CTCTTC 3 cut(s) 149, 357, 435
EclXI CGGCCG 1 cut(s) 275
Eco147I AGGCCT 1 cut(s) 466
Eco31I GGTCTC 1 cut(s) 422
Eco32I GATATC 1 cut(s) 358
Eco47I GGWCC 1 cut(s) 408
Eco52I CGGCCG 1 cut(s) 275
EcoRI GAATTC 1 cut(s) 322
EcoRII CCWGG 3 cut(s) 54, 221, 457
EcoRV GATATC 1 cut(s) 358
EcoT22I ATGCAT 1 cut(s) 390
FaeI CATG 7 cut(s) 145, 154, 190, 232, 290, 388, 403
FatI CATG 7 cut(s) 141, 150, 186, 228, 286, 384, 399
FauNDI CATATG 1 cut(s) 78
FbaI TGATCA 1 cut(s) 283
Fnu4HI GCNGC 7 cut(s) 21, 130, 192, 216, 248, 275, 485
FokI GGATG 1 cut(s) 365
Fsp4HI GCNGC 7 cut(s) 21, 130, 192, 216, 248, 275, 485
GluI GCNGC 7 cut(s) 21, 130, 192, 216, 248, 275, 485
HaeIII GGCC 7 cut(s) 191, 227, 277, 337, 374, 466, 473
HapII CCGG 4 cut(s) 11, 255, 278, 481
Hin1II CATG 7 cut(s) 145, 154, 190, 232, 290, 388, 403
HpaII CCGG 4 cut(s) 11, 255, 278, 481
HphI GGTGA 2 cut(s) 40, 196
Hpy166II GTNNAC 2 cut(s) 61, 136
Hpy188I TCNGA 2 cut(s) 184, 328
Hpy188III TCNNGA 1 cut(s) 287
Hpy8I GTNNAC 2 cut(s) 61, 136
HpyAV CCTTC 1 cut(s) 456
HpyCH4III ACNGT 1 cut(s) 172
HpyCH4IV ACGT 1 cut(s) 138
HpyCH4V TGCA 3 cut(s) 107, 232, 388
HpyF10VI GCNNNNNNNGC 4 cut(s) 104, 215, 224, 244
HpySE526I ACGT 1 cut(s) 138
Hsp92II CATG 7 cut(s) 145, 154, 190, 232, 290, 388, 403
KroI GCCGGC 1 cut(s) 277
KroNI GCCGGC 1 cut(s) 279
Ksp22I TGATCA 1 cut(s) 283
KspI CCGCGG 1 cut(s) 24
Kzo9I GATC 2 cut(s) 283, 292
LmnI GCTCC 1 cut(s) 223
Lsp1109I GCAGC 3 cut(s) 141, 259, 471
LweI GCATC 2 cut(s) 21, 397
MaeII ACGT 1 cut(s) 138
MaeIII GTNAC 1 cut(s) 172
MalI GATC 2 cut(s) 285, 294
MbiI CCGCTC 1 cut(s) 218
MboI GATC 2 cut(s) 283, 292
MboII GAAGA 4 cut(s) 166, 171, 374, 452
MluCI AATT 2 cut(s) 322, 346
MmeI TCCRAC 1 cut(s) 405
MnlI CCTC 6 cut(s) 19, 81, 150, 212, 436, 477
Mph1103I ATGCAT 1 cut(s) 390
MroNI GCCGGC 1 cut(s) 277
MseI TTAA 1 cut(s) 312
MspA1I CMGCKG 1 cut(s) 23
MspI CCGG 4 cut(s) 11, 255, 278, 481
MspR9I CCNGG 4 cut(s) 56, 223, 459, 481
MvaI CCWGG 3 cut(s) 56, 223, 459
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 4 cut(s) 104, 215, 224, 244
NaeI GCCGGC 1 cut(s) 279
NciI CCSGG 1 cut(s) 481
NdeI CATATG 1 cut(s) 78
NdeII GATC 2 cut(s) 283, 292
NgoMIV GCCGGC 1 cut(s) 277
NlaIII CATG 7 cut(s) 145, 154, 190, 232, 290, 388, 403
NlaIV GGNNCC 2 cut(s) 336, 375
NmeAIII GCCGAG 1 cut(s) 350
NmuCI GTSAC 1 cut(s) 172
NsiI ATGCAT 1 cut(s) 390
PagI TCATGA 1 cut(s) 286
PasI CCCWGGG 1 cut(s) 222
PceI AGGCCT 1 cut(s) 466
PdiI GCCGGC 1 cut(s) 279
PflMI CCANNNNNTGG 1 cut(s) 118
PfoI TCCNGGA 1 cut(s) 457
PinAI ACCGGT 1 cut(s) 10
PkrI GCNGC 7 cut(s) 22, 131, 193, 217, 249, 276, 486
PshBI ATTAAT 1 cut(s) 312
Psp6I CCWGG 3 cut(s) 54, 221, 457
PspGI CCWGG 3 cut(s) 54, 221, 457
PspN4I GGNNCC 2 cut(s) 336, 375
PspPI GGNCC 5 cut(s) 225, 335, 373, 408, 472
PstI CTGCAG 1 cut(s) 109
SacII CCGCGG 1 cut(s) 24
SaqAI TTAA 1 cut(s) 312
SatI GCNGC 7 cut(s) 21, 130, 192, 216, 248, 275, 485
Sau3AI GATC 2 cut(s) 283, 292
Sau96I GGNCC 5 cut(s) 225, 335, 373, 408, 472
ScrFI CCNGG 4 cut(s) 56, 223, 459, 481
SetI ASST 8 cut(s) 30, 92, 141, 252, 267, 345, 410, 497
SfaNI GCATC 2 cut(s) 21, 397
SfcI CTRYAG 2 cut(s) 105, 168
Sfr303I CCGCGG 1 cut(s) 24
SgrBI CCGCGG 1 cut(s) 24
SinI GGWCC 1 cut(s) 408
Sse9I AATT 2 cut(s) 322, 346
SseBI AGGCCT 1 cut(s) 466
SsiI CCGC 6 cut(s) 21, 23, 192, 207, 216, 274
StuI AGGCCT 1 cut(s) 466
StyD4I CCNGG 4 cut(s) 54, 221, 457, 479
TaaI ACNGT 1 cut(s) 172
TaiI ACGT 1 cut(s) 141
TaqI TCGA 3 cut(s) 36, 360, 391
TasI AATT 2 cut(s) 322, 346
TauI GCSGC 4 cut(s) 23, 194, 218, 277
Tru1I TTAA 1 cut(s) 312
Tru9I TTAA 1 cut(s) 312
TseFI GTSAC 1 cut(s) 172
TseI GCWGC 3 cut(s) 129, 247, 484
Tsp45I GTSAC 1 cut(s) 172
TspDTI ATGAA 5 cut(s) 17, 21, 167, 335, 373
Van91I CCANNNNNTGG 1 cut(s) 118
VpaK11BI GGWCC 1 cut(s) 408
VspI ATTAAT 1 cut(s) 312
XapI RAATTY 2 cut(s) 322, 346
Zsp2I ATGCAT 1 cut(s) 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.