RchiOBHm_Chr4g0433741

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0003619
Physical Location & Seq
Reverse (-)
57754542 .. 57755420
879 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40221

Sequence Viewer

Length: 549 bp
ATGGGGAACTACATCTCATGCACTCTAGCCACGCCTCTCATCAAGAGCACCAAGGCGGCGCGTGTGGTCTTTCCTACCGGCGAAGTAAGGCAGTTCCGTGAACCGATCAAGGCCGCAGAGCTCATGCTGGAGTCGCCGAACACGTTCCTAGCCAACTCCAGGTCTCTCCACATCGGCAGGCGATTCTCCGCCCTCGCCGCCGACGAGGAGCTCGAGTTCGGCAACGTCTACATCATGTTCCCGATGCGGCGGCTCAGCTCCATCGTCACCGCTGCCGACATGACCATCTTTTTCATCGCGGCCAACTCCGCTGCCAAGCGGATTTCCGGCGGGAAGGTCAGGATCTTGCCTGAGGCTCAAGCAGTCCAAAACGACGAGGAGGAGGAGAAAAGTCAAAGCGGTGACGGAGAAGGTCAAGGTCCGGCGAGGCTGAGCCTGGACGGAGTTGAGGGTTTGCAAGTGGCGGAGTTCCAGTATAGATTAGCTGCGTGTAGGTCAAGGAAGCCAATGTTGGAGACGATCAAAGAGGAGCTGATTTTCTCAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

182

Amino Acids

20.09

Weight (kDa)

6.63

Isoelectric Point (pI)

51.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 176 5.9e-39 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 228
AccII CGCG 2 cut(s) 61, 299
AclWI GGATC 1 cut(s) 350
AcoI YGGCCR 1 cut(s) 300
AfiI CCNNNNNNNGG 1 cut(s) 159
AflIII ACRYGT 1 cut(s) 141
AjnI CCWGG 2 cut(s) 158, 435
AjuI GAANNNNNNNTTGG 2 cut(s) 494, 526
AloI GAACNNNNNNTCC 2 cut(s) 200, 232
AluBI AGCT 5 cut(s) 121, 211, 258, 485, 532
AluI AGCT 5 cut(s) 121, 211, 258, 485, 532
Alw21I GWGCWC 3 cut(s) 50, 123, 213
Alw26I GTCTC 2 cut(s) 168, 509
AlwI GGATC 1 cut(s) 350
Ama87I CYCGRG 1 cut(s) 212
AoxI GGCC 2 cut(s) 111, 300
ApeKI GCWGC 3 cut(s) 272, 311, 485
Asp700I GAANNNNTTC 1 cut(s) 143
AspLEI GCGC 1 cut(s) 61
AspS9I GGNCC 1 cut(s) 419
AsuHPI GGTGA 2 cut(s) 259, 413
AvaI CYCGRG 1 cut(s) 212
AvaII GGWCC 1 cut(s) 419
AxyI CCTNAGG 1 cut(s) 351
BanII GRGCYC 2 cut(s) 123, 213
Bbv12I GWGCWC 3 cut(s) 50, 123, 213
BbvI GCAGC 3 cut(s) 259, 298, 472
BccI CCATC 2 cut(s) 269, 293
BciT130I CCWGG 2 cut(s) 160, 437
BcoDI GTCTC 2 cut(s) 168, 509
BfaI CTAG 2 cut(s) 26, 149
BisI GCNGC 9 cut(s) 57, 114, 198, 248, 251, 273, 300, 312, 486
BlpI GCTNAGC 2 cut(s) 254, 431
BlsI GCNGC 9 cut(s) 58, 115, 199, 249, 252, 274, 301, 313, 487
Bme1390I CCNGG 2 cut(s) 160, 437
Bme18I GGWCC 1 cut(s) 419
BmeT110I CYCGRG 1 cut(s) 212
BmgT120I GGNCC 1 cut(s) 419
BmrFI CCNGG 2 cut(s) 160, 437
BmsI GCATC 1 cut(s) 234
BpmI CTGGAG 2 cut(s) 142, 149
Bpu1102I GCTNAGC 2 cut(s) 254, 431
BpuEI CTTGAG 2 cut(s) 342, 526
BsaI GGTCTC 1 cut(s) 168
BsaJI CCNNGG 1 cut(s) 51
BsaXI ACNNNNNCTCC 4 cut(s) 200, 230, 458, 488
Bsc4I CCNNNNNNNGG 1 cut(s) 159
Bse118I RCCGGY 1 cut(s) 77
Bse1I ACTGG 1 cut(s) 472
Bse21I CCTNAGG 1 cut(s) 351
BseBI CCWGG 2 cut(s) 160, 437
BseDI CCNNGG 1 cut(s) 51
BseLI CCNNNNNNNGG 1 cut(s) 159
BseMII CTCAG 3 cut(s) 268, 342, 422
BseNI ACTGG 1 cut(s) 472
BseRI GAGGAG 5 cut(s) 221, 392, 395, 398, 542
BseXI GCAGC 3 cut(s) 259, 298, 472
Bsh1236I CGCG 2 cut(s) 61, 299
BshFI GGCC 2 cut(s) 113, 302
BsiHKAI GWGCWC 3 cut(s) 50, 123, 213
BsiHKCI CYCGRG 1 cut(s) 212
BsiSI CCGG 3 cut(s) 78, 327, 422
BslI CCNNNNNNNGG 1 cut(s) 159
BsmAI GTCTC 2 cut(s) 168, 509
BsmBI CGTCTC 1 cut(s) 509
BsnI GGCC 2 cut(s) 113, 302
Bso31I GGTCTC 1 cut(s) 168
BsoBI CYCGRG 1 cut(s) 212
Bsp1286I GDGCHC 3 cut(s) 50, 123, 213
Bsp143I GATC 3 cut(s) 105, 342, 519
Bsp1720I GCTNAGC 2 cut(s) 254, 431
BspANI GGCC 2 cut(s) 113, 302
BspCNI CTCAG 3 cut(s) 267, 343, 423
BspFNI CGCG 2 cut(s) 61, 299
BspPI GGATC 1 cut(s) 350
BspTNI GGTCTC 1 cut(s) 168
BsrFI RCCGGY 1 cut(s) 77
BsrI ACTGG 1 cut(s) 472
BssAI RCCGGY 1 cut(s) 77
BssECI CCNNGG 1 cut(s) 51
BssMI GATC 3 cut(s) 105, 342, 519
BssT1I CCWWGG 1 cut(s) 51
Bst2UI CCWGG 2 cut(s) 160, 437
BstC8I GCNNGC 1 cut(s) 179
BstDEI CTNAG 3 cut(s) 254, 351, 431
BstFNI CGCG 2 cut(s) 61, 299
BstHHI GCGC 1 cut(s) 61
BstKTI GATC 3 cut(s) 108, 345, 522
BstMAI GTCTC 2 cut(s) 168, 509
BstMBI GATC 3 cut(s) 105, 342, 519
BstMWI GCNNNNNNNGC 3 cut(s) 133, 197, 308
BstNI CCWGG 2 cut(s) 160, 437
BstSCI CCNGG 2 cut(s) 158, 435
BstUI CGCG 2 cut(s) 61, 299
BstV1I GCAGC 3 cut(s) 259, 298, 472
BstX2I RGATCY 1 cut(s) 342
BstYI RGATCY 1 cut(s) 342
Bsu36I CCTNAGG 1 cut(s) 351
BsuRI GGCC 2 cut(s) 113, 302
BtgZI GCGATG 1 cut(s) 280
Cac8I GCNNGC 1 cut(s) 179
CfoI GCGC 1 cut(s) 61
Cfr10I RCCGGY 1 cut(s) 77
Cfr13I GGNCC 1 cut(s) 419
CviAII CATG 4 cut(s) 18, 124, 235, 280
DdeI CTNAG 3 cut(s) 254, 351, 431
DpnI GATC 3 cut(s) 107, 344, 521
DpnII GATC 3 cut(s) 105, 342, 519
EaeI YGGCCR 1 cut(s) 300
EciI GGCGGA 2 cut(s) 178, 479
Ecl136II GAGCTC 2 cut(s) 121, 211
Eco130I CCWWGG 1 cut(s) 51
Eco24I GRGCYC 2 cut(s) 123, 213
Eco31I GGTCTC 1 cut(s) 168
Eco47I GGWCC 1 cut(s) 419
Eco53kI GAGCTC 2 cut(s) 121, 211
Eco81I CCTNAGG 1 cut(s) 351
Eco88I CYCGRG 1 cut(s) 212
EcoICRI GAGCTC 2 cut(s) 121, 211
EcoRII CCWGG 2 cut(s) 158, 435
EcoT14I CCWWGG 1 cut(s) 51
EcoT38I GRGCYC 2 cut(s) 123, 213
ErhI CCWWGG 1 cut(s) 51
Esp3I CGTCTC 1 cut(s) 509
FaeI CATG 4 cut(s) 21, 127, 238, 283
FaiI YATR 5 cut(s) 19, 125, 236, 281, 477
FatI CATG 4 cut(s) 17, 123, 234, 279
FauI CCCGC 1 cut(s) 323
FblI GTMKAC 1 cut(s) 228
Fnu4HI GCNGC 9 cut(s) 57, 114, 198, 248, 251, 273, 300, 312, 486
FriOI GRGCYC 2 cut(s) 123, 213
Fsp4HI GCNGC 9 cut(s) 57, 114, 198, 248, 251, 273, 300, 312, 486
FspBI CTAG 2 cut(s) 26, 149
GlaI GCGC 1 cut(s) 60
GluI GCNGC 9 cut(s) 57, 114, 198, 248, 251, 273, 300, 312, 486
GsuI CTGGAG 2 cut(s) 142, 149
HaeIII GGCC 2 cut(s) 113, 302
HapII CCGG 3 cut(s) 78, 327, 422
HhaI GCGC 1 cut(s) 61
Hin1II CATG 4 cut(s) 21, 127, 238, 283
Hin6I GCGC 1 cut(s) 59
HinP1I GCGC 1 cut(s) 59
HinfI GANTC 2 cut(s) 131, 183
HpaII CCGG 3 cut(s) 78, 327, 422
HphI GGTGA 2 cut(s) 259, 413
Hpy166II GTNNAC 2 cut(s) 101, 229
Hpy188III TCNNGA 4 cut(s) 43, 241, 340, 543
Hpy8I GTNNAC 2 cut(s) 101, 229
Hpy99I CGWCG 2 cut(s) 206, 377
HpyAV CCTTC 2 cut(s) 328, 404
HpyCH4IV ACGT 2 cut(s) 143, 225
HpyCH4V TGCA 2 cut(s) 21, 457
HpyF10VI GCNNNNNNNGC 3 cut(s) 133, 197, 308
HpyF3I CTNAG 3 cut(s) 254, 351, 431
HpySE526I ACGT 2 cut(s) 143, 225
Hsp92II CATG 4 cut(s) 21, 127, 238, 283
HspAI GCGC 1 cut(s) 59
Kzo9I GATC 3 cut(s) 105, 342, 519
LmnI GCTCC 3 cut(s) 208, 263, 529
Lsp1109I GCAGC 3 cut(s) 259, 298, 472
LweI GCATC 1 cut(s) 234
MaeI CTAG 2 cut(s) 26, 149
MaeII ACGT 2 cut(s) 143, 225
MaeIII GTNAC 2 cut(s) 265, 401
MalI GATC 3 cut(s) 107, 344, 521
MboI GATC 3 cut(s) 105, 342, 519
MflI RGATCY 1 cut(s) 342
MhlI GDGCHC 3 cut(s) 50, 123, 213
MlyI GAGTC 1 cut(s) 140
MmeI TCCRAC 1 cut(s) 492
MroXI GAANNNNTTC 1 cut(s) 143
MspA1I CMGCKG 2 cut(s) 272, 311
MspI CCGG 3 cut(s) 78, 327, 422
MspR9I CCNGG 2 cut(s) 160, 437
MvaI CCWGG 2 cut(s) 160, 437
MvnI CGCG 2 cut(s) 61, 299
MwoI GCNNNNNNNGC 3 cut(s) 133, 197, 308
NdeII GATC 3 cut(s) 105, 342, 519
NlaIII CATG 4 cut(s) 21, 127, 238, 283
NmuCI GTSAC 2 cut(s) 265, 401
PaeR7I CTCGAG 1 cut(s) 212
PcsI WCGNNNNNNNCGW 3 cut(s) 140, 201, 210
PdmI GAANNNNTTC 1 cut(s) 143
PfeI GAWTC 1 cut(s) 183
PkrI GCNGC 9 cut(s) 58, 115, 199, 249, 252, 274, 301, 313, 487
PleI GAGTC 1 cut(s) 139
PpsI GAGTC 1 cut(s) 139
Psp124BI GAGCTC 2 cut(s) 123, 213
Psp6I CCWGG 2 cut(s) 158, 435
PspGI CCWGG 2 cut(s) 158, 435
PspPI GGNCC 1 cut(s) 419
PspXI VCTCGAGB 1 cut(s) 212
PsuI RGATCY 1 cut(s) 342
SacI GAGCTC 2 cut(s) 123, 213
SatI GCNGC 9 cut(s) 57, 114, 198, 248, 251, 273, 300, 312, 486
Sau3AI GATC 3 cut(s) 105, 342, 519
Sau96I GGNCC 1 cut(s) 419
SchI GAGTC 1 cut(s) 140
ScrFI CCNGG 2 cut(s) 160, 437
SduI GDGCHC 3 cut(s) 50, 123, 213
SfaNI GCATC 1 cut(s) 234
Sfr274I CTCGAG 1 cut(s) 212
SinI GGWCC 1 cut(s) 419
SlaI CTCGAG 1 cut(s) 212
SmlI CTYRAG 3 cut(s) 212, 357, 541
SmoI CTYRAG 3 cut(s) 212, 357, 541
SspMI CTAG 2 cut(s) 26, 149
SstI GAGCTC 2 cut(s) 123, 213
StyD4I CCNGG 2 cut(s) 158, 435
StyI CCWWGG 1 cut(s) 51
TaiI ACGT 2 cut(s) 146, 228
TaqI TCGA 1 cut(s) 213
TauI GCSGC 6 cut(s) 59, 116, 200, 250, 253, 302
TfiI GAWTC 1 cut(s) 183
TseFI GTSAC 2 cut(s) 265, 401
TseI GCWGC 3 cut(s) 272, 311, 485
Tsp45I GTSAC 2 cut(s) 265, 401
TspDTI ATGAA 1 cut(s) 283
TspGWI ACGGA 3 cut(s) 86, 420, 456
VpaK11BI GGWCC 1 cut(s) 419
XhoI CTCGAG 1 cut(s) 212
XmiI GTMKAC 1 cut(s) 228
XmnI GAANNNNTTC 1 cut(s) 143
XspI CTAG 2 cut(s) 26, 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.