Prupe.1G321600_v2.0.a1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0003619
Physical Location & Seq
Forward (+)
30795607 .. 30796484
878 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G321600.1

Sequence Viewer

Length: 573 bp
ATGGGAAACTACATCTCATGCACTTTGGCCACGCCTCTGATCAAGAGCTCCAAATCAGCAAGAGTGATCTTCCCAAACAGCGCCGTTCGACAGTTCCGGGAACCGATCAAAGCGGCGGAGCTCATGCTCGAGTGTCCAGGCACCTTCTTAGCAAACTCACAGTCTCTCCACATAGGCCGCAGATTCTCCGCTCTCGGTGCCGACGAGGAGTTGGAGTTTGGCAATGTTTACCTCATGTTCCCGATGAGGAGGCTCAGCTCTATAGTCACGGCCGCAGACATGGCAGTGTTCTTCATGGCGGCCAACTCAGCGGCCAAGCGCATATCTGGCGGCAAGGTCAGGATCTTGCCCGAAAACGGCCGCGAAGGAGGAGAAATGGTAGGAGTGGAGAGCGGGGAAGAAAGTCATGATGATCAAGATCAAGATGGTAAAGGTGATCGAGGTCCACTGAGGTTGAGTTTGGATGGAGTTGAGGGGTTGCAGGTGGCAGAGTTTCAGTATAGGTTAGCTGCCTGTAGGTCAAGGAAGCCTGTATTGGAAACCATCAAAGAAGAGCCGATTTCTTCAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

191

Amino Acids

20.77

Weight (kDa)

6.18

Isoelectric Point (pI)

50.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 472
Acc36I ACCTGC 1 cut(s) 472
AccB1I GGYRCC 2 cut(s) 140, 197
AccBSI CCGCTC 2 cut(s) 191, 393
AccII CGCG 1 cut(s) 363
AclWI GGATC 1 cut(s) 350
AcoI YGGCCR 5 cut(s) 27, 270, 300, 312, 358
AfiI CCNNNNNNNGG 1 cut(s) 356
AgsI TTSAA 1 cut(s) 567
AjnI CCWGG 1 cut(s) 136
AjuI GAANNNNNNNTTGG 2 cut(s) 518, 550
AluBI AGCT 4 cut(s) 48, 121, 258, 509
AluI AGCT 4 cut(s) 48, 121, 258, 509
Alw21I GWGCWC 2 cut(s) 50, 123
Alw26I GTCTC 1 cut(s) 168
AlwI GGATC 1 cut(s) 350
Ama87I CYCGRG 1 cut(s) 128
AoxI GGCC 6 cut(s) 27, 175, 270, 300, 312, 358
ApeKI GCWGC 1 cut(s) 509
ArsI GACNNNNNNTTYG 2 cut(s) 146, 178
AspLEI GCGC 2 cut(s) 83, 321
AspS9I GGNCC 1 cut(s) 443
AsuC2I CCSGG 1 cut(s) 98
AsuHPI GGTGA 1 cut(s) 446
AvaI CYCGRG 1 cut(s) 128
AvaII GGWCC 1 cut(s) 443
BalI TGGCCA 1 cut(s) 29
BanI GGYRCC 2 cut(s) 140, 197
BanII GRGCYC 2 cut(s) 50, 123
Bbv12I GWGCWC 2 cut(s) 50, 123
BbvI GCAGC 1 cut(s) 496
BccI CCATC 3 cut(s) 419, 458, 551
BceAI ACGGC 3 cut(s) 68, 285, 373
BciT130I CCWGG 1 cut(s) 138
BclI TGATCA 2 cut(s) 39, 412
BcnI CCSGG 1 cut(s) 98
BcoDI GTCTC 1 cut(s) 168
BfmI CTRYAG 2 cut(s) 261, 514
BfoI RGCGCY 1 cut(s) 84
BfuAI ACCTGC 1 cut(s) 472
BisI GCNGC 8 cut(s) 114, 178, 273, 300, 312, 331, 361, 510
BlpI GCTNAGC 1 cut(s) 254
BlsI GCNGC 8 cut(s) 115, 179, 274, 301, 313, 332, 362, 511
Bme1390I CCNGG 2 cut(s) 98, 138
Bme18I GGWCC 1 cut(s) 443
BmeT110I CYCGRG 1 cut(s) 128
BmgT120I GGNCC 1 cut(s) 443
BmiI GGNNCC 3 cut(s) 102, 142, 199
BmrFI CCNGG 2 cut(s) 98, 138
Bpu1102I GCTNAGC 1 cut(s) 254
BpuMI CCSGG 1 cut(s) 98
BsaBI GATNNNNATC 1 cut(s) 417
BsaXI ACNNNNNCTCC 6 cut(s) 150, 180, 200, 230, 363, 393
Bsc4I CCNNNNNNNGG 1 cut(s) 356
Bse3DI GCAATG 1 cut(s) 229
Bse8I GATNNNNATC 1 cut(s) 417
BseBI CCWGG 1 cut(s) 138
BseGI GGATG 1 cut(s) 469
BseJI GATNNNNATC 1 cut(s) 417
BseLI CCNNNNNNNGG 1 cut(s) 356
BseMI GCAATG 1 cut(s) 229
BseMII CTCAG 3 cut(s) 268, 321, 440
BseRI GAGGAG 3 cut(s) 221, 262, 384
BseX3I CGGCCG 2 cut(s) 270, 358
BseXI GCAGC 1 cut(s) 496
Bsh1236I CGCG 1 cut(s) 363
Bsh1285I CGRYCG 2 cut(s) 273, 361
BshFI GGCC 6 cut(s) 29, 177, 272, 302, 314, 360
BshNI GGYRCC 2 cut(s) 140, 197
BsiEI CGRYCG 2 cut(s) 273, 361
BsiHKAI GWGCWC 2 cut(s) 50, 123
BsiHKCI CYCGRG 1 cut(s) 128
BsiSI CCGG 1 cut(s) 97
BslI CCNNNNNNNGG 1 cut(s) 356
BsmAI GTCTC 1 cut(s) 168
BsnI GGCC 6 cut(s) 29, 177, 272, 302, 314, 360
BsoBI CYCGRG 1 cut(s) 128
Bsp1286I GDGCHC 2 cut(s) 50, 123
Bsp143I GATC 7 cut(s) 39, 66, 105, 342, 412, 418, 436
Bsp1720I GCTNAGC 1 cut(s) 254
BspANI GGCC 6 cut(s) 29, 177, 272, 302, 314, 360
BspCNI CTCAG 3 cut(s) 267, 320, 441
BspFNI CGCG 1 cut(s) 363
BspHI TCATGA 1 cut(s) 406
BspLI GGNNCC 3 cut(s) 102, 142, 199
BspMI ACCTGC 1 cut(s) 472
BspPI GGATC 1 cut(s) 350
BspQI GCTCTTC 1 cut(s) 546
BspT107I GGYRCC 2 cut(s) 140, 197
BsrBI CCGCTC 2 cut(s) 191, 393
BsrDI GCAATG 1 cut(s) 229
BssMI GATC 7 cut(s) 39, 66, 105, 342, 412, 418, 436
Bst2UI CCWGG 1 cut(s) 138
Bst4CI ACNGT 2 cut(s) 93, 162
Bst6I CTCTTC 1 cut(s) 546
BstDEI CTNAG 4 cut(s) 148, 254, 307, 449
BstF5I GGATG 1 cut(s) 469
BstFNI CGCG 1 cut(s) 363
BstH2I RGCGCY 1 cut(s) 84
BstHHI GCGC 2 cut(s) 83, 321
BstKTI GATC 7 cut(s) 42, 69, 108, 345, 415, 421, 439
BstMAI GTCTC 1 cut(s) 168
BstMBI GATC 7 cut(s) 39, 66, 105, 342, 412, 418, 436
BstMCI CGRYCG 2 cut(s) 273, 361
BstMWI GCNNNNNNNGC 4 cut(s) 197, 281, 308, 327
BstNI CCWGG 1 cut(s) 138
BstSCI CCNGG 2 cut(s) 96, 136
BstSFI CTRYAG 2 cut(s) 261, 514
BstUI CGCG 1 cut(s) 363
BstV1I GCAGC 1 cut(s) 496
BstX2I RGATCY 1 cut(s) 342
BstYI RGATCY 1 cut(s) 342
BstZI CGGCCG 2 cut(s) 270, 358
BsuRI GGCC 6 cut(s) 29, 177, 272, 302, 314, 360
BtsCI GGATG 1 cut(s) 469
BtsI GCAGTG 1 cut(s) 291
BtsIMutI CAGTG 2 cut(s) 291, 446
BveI ACCTGC 1 cut(s) 472
CciI TCATGA 1 cut(s) 406
CfoI GCGC 2 cut(s) 83, 321
Cfr13I GGNCC 1 cut(s) 443
CspCI CAANNNNNGTGG 2 cut(s) 435, 470
CviAII CATG 6 cut(s) 18, 124, 235, 280, 295, 407
DdeI CTNAG 4 cut(s) 148, 254, 307, 449
DpnI GATC 7 cut(s) 41, 68, 107, 344, 414, 420, 438
DpnII GATC 7 cut(s) 39, 66, 105, 342, 412, 418, 436
EaeI YGGCCR 5 cut(s) 27, 270, 300, 312, 358
EagI CGGCCG 2 cut(s) 270, 358
Eam1104I CTCTTC 1 cut(s) 546
EarI CTCTTC 1 cut(s) 546
EciI GGCGGA 1 cut(s) 131
Ecl136II GAGCTC 2 cut(s) 48, 121
EclXI CGGCCG 2 cut(s) 270, 358
Eco24I GRGCYC 2 cut(s) 50, 123
Eco47I GGWCC 1 cut(s) 443
Eco52I CGGCCG 2 cut(s) 270, 358
Eco53kI GAGCTC 2 cut(s) 48, 121
Eco88I CYCGRG 1 cut(s) 128
EcoICRI GAGCTC 2 cut(s) 48, 121
EcoRII CCWGG 1 cut(s) 136
EcoT38I GRGCYC 2 cut(s) 50, 123
FaeI CATG 6 cut(s) 21, 127, 238, 283, 298, 410
FatI CATG 6 cut(s) 17, 123, 234, 279, 294, 406
FauI CCCGC 1 cut(s) 386
FbaI TGATCA 2 cut(s) 39, 412
Fnu4HI GCNGC 8 cut(s) 114, 178, 273, 300, 312, 331, 361, 510
FokI GGATG 1 cut(s) 476
FriOI GRGCYC 2 cut(s) 50, 123
Fsp4HI GCNGC 8 cut(s) 114, 178, 273, 300, 312, 331, 361, 510
GlaI GCGC 2 cut(s) 82, 320
GluI GCNGC 8 cut(s) 114, 178, 273, 300, 312, 331, 361, 510
HaeII RGCGCY 1 cut(s) 84
HaeIII GGCC 6 cut(s) 29, 177, 272, 302, 314, 360
HapII CCGG 1 cut(s) 97
HhaI GCGC 2 cut(s) 83, 321
Hin1II CATG 6 cut(s) 21, 127, 238, 283, 298, 410
Hin6I GCGC 2 cut(s) 81, 319
HinP1I GCGC 2 cut(s) 81, 319
HinfI GANTC 1 cut(s) 183
HpaII CCGG 1 cut(s) 97
HphI GGTGA 1 cut(s) 446
Hpy166II GTNNAC 2 cut(s) 229, 446
Hpy188I TCNGA 1 cut(s) 39
Hpy188III TCNNGA 7 cut(s) 43, 241, 340, 407, 416, 422, 567
Hpy8I GTNNAC 2 cut(s) 229, 446
Hpy99I CGWCG 1 cut(s) 206
HpyAV CCTTC 2 cut(s) 154, 359
HpyCH4III ACNGT 2 cut(s) 93, 162
HpyCH4V TGCA 2 cut(s) 21, 481
HpyF10VI GCNNNNNNNGC 4 cut(s) 197, 281, 308, 327
HpyF3I CTNAG 4 cut(s) 148, 254, 307, 449
Hsp92II CATG 6 cut(s) 21, 127, 238, 283, 298, 410
HspAI GCGC 2 cut(s) 81, 319
Ksp22I TGATCA 2 cut(s) 39, 412
Kzo9I GATC 7 cut(s) 39, 66, 105, 342, 412, 418, 436
LguI GCTCTTC 1 cut(s) 546
LmnI GCTCC 2 cut(s) 53, 118
LpnPI CCDG 8 cut(s) 110, 123, 150, 312, 325, 467, 526, 543
Lsp1109I GCAGC 1 cut(s) 496
MaeIII GTNAC 1 cut(s) 265
MalI GATC 7 cut(s) 41, 68, 107, 344, 414, 420, 438
MbiI CCGCTC 2 cut(s) 191, 393
MboI GATC 7 cut(s) 39, 66, 105, 342, 412, 418, 436
MboII GAAGA 5 cut(s) 61, 283, 410, 555, 563
MflI RGATCY 1 cut(s) 342
MhlI GDGCHC 2 cut(s) 50, 123
MlsI TGGCCA 1 cut(s) 29
MluNI TGGCCA 1 cut(s) 29
MmeI TCCRAC 1 cut(s) 192
MnlI CCTC 9 cut(s) 45, 199, 240, 242, 243, 362, 434, 444, 466
Mox20I TGGCCA 1 cut(s) 29
MscI TGGCCA 1 cut(s) 29
MslI CAYNNNNRTG 1 cut(s) 284
Msp20I TGGCCA 1 cut(s) 29
MspA1I CMGCKG 1 cut(s) 311
MspI CCGG 1 cut(s) 97
MspR9I CCNGG 2 cut(s) 98, 138
MvaI CCWGG 1 cut(s) 138
MvnI CGCG 1 cut(s) 363
MwoI GCNNNNNNNGC 4 cut(s) 197, 281, 308, 327
NciI CCSGG 1 cut(s) 98
NdeII GATC 7 cut(s) 39, 66, 105, 342, 412, 418, 436
NlaIII CATG 6 cut(s) 21, 127, 238, 283, 298, 410
NlaIV GGNNCC 3 cut(s) 102, 142, 199
NmuCI GTSAC 1 cut(s) 265
PaeR7I CTCGAG 1 cut(s) 128
PagI TCATGA 1 cut(s) 406
PaqCI CACCTGC 1 cut(s) 472
PciSI GCTCTTC 1 cut(s) 546
PcsI WCGNNNNNNNCGW 1 cut(s) 201
PfeI GAWTC 1 cut(s) 183
PfoI TCCNGGA 1 cut(s) 96
PkrI GCNGC 8 cut(s) 115, 179, 274, 301, 313, 332, 362, 511
Psp124BI GAGCTC 2 cut(s) 50, 123
Psp6I CCWGG 1 cut(s) 136
PspGI CCWGG 1 cut(s) 136
PspN4I GGNNCC 3 cut(s) 102, 142, 199
PspPI GGNCC 1 cut(s) 443
PspXI VCTCGAGB 1 cut(s) 128
PsuI RGATCY 1 cut(s) 342
RseI CAYNNNNRTG 1 cut(s) 284
SacI GAGCTC 2 cut(s) 50, 123
SapI GCTCTTC 1 cut(s) 546
SatI GCNGC 8 cut(s) 114, 178, 273, 300, 312, 331, 361, 510
Sau3AI GATC 7 cut(s) 39, 66, 105, 342, 412, 418, 436
Sau96I GGNCC 1 cut(s) 443
ScrFI CCNGG 2 cut(s) 98, 138
SduI GDGCHC 2 cut(s) 50, 123
SfcI CTRYAG 2 cut(s) 261, 514
Sfr274I CTCGAG 1 cut(s) 128
SinI GGWCC 1 cut(s) 443
SlaI CTCGAG 1 cut(s) 128
SmiMI CAYNNNNRTG 1 cut(s) 284
SmlI CTYRAG 1 cut(s) 128
SmoI CTYRAG 1 cut(s) 128
SstI GAGCTC 2 cut(s) 50, 123
StyD4I CCNGG 2 cut(s) 96, 136
TaaI ACNGT 2 cut(s) 93, 162
TaqI TCGA 3 cut(s) 88, 129, 439
TauI GCSGC 7 cut(s) 116, 180, 275, 302, 314, 333, 363
TfiI GAWTC 1 cut(s) 183
TscAI CASTG 2 cut(s) 291, 453
TseFI GTSAC 1 cut(s) 265
TseI GCWGC 1 cut(s) 509
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 1 cut(s) 283
TspRI CASTG 2 cut(s) 291, 453
VpaK11BI GGWCC 1 cut(s) 443
XhoI CTCGAG 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.