MD09G1258400.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0003619
Physical Location & Seq
Reverse (-)
33038772 .. 33039368
597 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1258400.v1.1.491

Sequence Viewer

Length: 597 bp
ATGGGCAACTACATTTCTTGTAGCCTAGCAACCCAAATATTGAGCAAGCATGGGAAAGCAGCAGCCACTAAAGTGATCGTCCCAAGCGGTGAAATCAAACAATTCAATGTCCCCATAAAAGCCGCCGAGCTGATGGTCGACACCCCAAACTTCTTCCTCGTAAACATCAAGTCTCTCCATGTGGGAAGAAGATTTTCGCATCTCAGTGCCGACGAAGAGTTGGATATCGGCAACGTTTACGTCATGTTCCCCATGAAGAGGCTCAACTCCACTGTCACAACAGCTGATATGGGGCCACTGTTCCTCACCGCCGCAGCTGGGTCAGCAAGCAAGCGAGCTTCCGGGAGAAACGTGAGGGTGCGGCGGGCTGATCATCGTCAATCCATGAAGTTTGGTAATGAAGGCGATGAGTGTAAAAGGATTAAGAGTTGGGGGAAAAGTTTGGAGCATGAGGCGGCGGCGCCAAAGCTTAACTTGGATGACATCGAAGATTTCTCGGCGCCGGAGTTTATGCACAGGTTGTCTATGAGTAGGTCAAAGAAGCCATTGCTGGATACCATAGCAGAAGAGCCTGCAGTTTCTGTTTCTTCAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

199

Amino Acids

21.79

Weight (kDa)

9.42

Isoelectric Point (pI)

44.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 189 2.1e-36 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 460, 499
AccI GTMKAC 1 cut(s) 138
AciI CCGC 8 cut(s) 87, 123, 309, 312, 361, 364, 455, 458
AclI AACGTT 1 cut(s) 234
AcyI GRCGYC 2 cut(s) 461, 500
AfiI CCNNNNNNNGG 2 cut(s) 258, 318
AgsI TTSAA 2 cut(s) 106, 591
AleI CACNNNNGTG 1 cut(s) 71
AluBI AGCT 5 cut(s) 130, 284, 317, 338, 469
AluI AGCT 5 cut(s) 130, 284, 317, 338, 469
Alw26I GTCTC 1 cut(s) 177
AlwNI CAGNNNCTG 1 cut(s) 581
AoxI GGCC 1 cut(s) 293
ApeKI GCWGC 3 cut(s) 59, 62, 314
Asp700I GAANNNNTTC 1 cut(s) 193
AspLEI GCGC 2 cut(s) 463, 502
AspS9I GGNCC 1 cut(s) 293
AsuC2I CCSGG 1 cut(s) 343
AsuHPI GGTGA 2 cut(s) 101, 298
BanI GGYRCC 2 cut(s) 460, 499
BbvI GCAGC 3 cut(s) 71, 74, 326
BccI CCATC 1 cut(s) 127
BciVI GTATCC 1 cut(s) 547
BclI TGATCA 1 cut(s) 370
BcnI CCSGG 1 cut(s) 343
BcoDI GTCTC 1 cut(s) 177
BfaI CTAG 1 cut(s) 26
BfmI CTRYAG 1 cut(s) 573
BfoI RGCGCY 2 cut(s) 464, 503
BfuI GTATCC 1 cut(s) 547
BisI GCNGC 8 cut(s) 60, 63, 123, 312, 315, 362, 456, 459
BlsI GCNGC 8 cut(s) 61, 64, 124, 313, 316, 363, 457, 460
Bme1390I CCNGG 1 cut(s) 343
BmgT120I GGNCC 1 cut(s) 293
BmiI GGNNCC 3 cut(s) 294, 462, 501
BmrFI CCNGG 1 cut(s) 343
BmsI GCATC 1 cut(s) 208
BpuMI CCSGG 1 cut(s) 343
BsaHI GRCGYC 2 cut(s) 461, 500
Bsc4I CCNNNNNNNGG 2 cut(s) 258, 318
Bse3DI GCAATG 1 cut(s) 545
BseGI GGATG 1 cut(s) 484
BseLI CCNNNNNNNGG 2 cut(s) 258, 318
BseMI GCAATG 1 cut(s) 545
BseMII CTCAG 1 cut(s) 217
BseXI GCAGC 3 cut(s) 71, 74, 326
BseYI CCCAGC 1 cut(s) 317
BshFI GGCC 1 cut(s) 295
BshNI GGYRCC 2 cut(s) 460, 499
BsiSI CCGG 2 cut(s) 342, 503
BslFI GGGAC 2 cut(s) 65, 95
BslI CCNNNNNNNGG 2 cut(s) 258, 318
BsmAI GTCTC 1 cut(s) 177
BsmFI GGGAC 2 cut(s) 65, 95
BsnI GGCC 1 cut(s) 295
Bsp143I GATC 2 cut(s) 75, 370
BspACI CCGC 8 cut(s) 87, 123, 309, 312, 361, 364, 455, 458
BspANI GGCC 1 cut(s) 295
BspCNI CTCAG 1 cut(s) 216
BspLI GGNNCC 3 cut(s) 294, 462, 501
BspMAI CTGCAG 1 cut(s) 577
BspQI GCTCTTC 1 cut(s) 561
BspT107I GGYRCC 2 cut(s) 460, 499
BsrDI GCAATG 1 cut(s) 545
BssMI GATC 2 cut(s) 75, 370
BssNI GRCGYC 2 cut(s) 461, 500
Bst4CI ACNGT 2 cut(s) 274, 300
Bst6I CTCTTC 3 cut(s) 210, 251, 561
BstACI GRCGYC 2 cut(s) 461, 500
BstC8I GCNNGC 6 cut(s) 47, 328, 332, 336, 366, 573
BstDEI CTNAG 1 cut(s) 203
BstF5I GGATG 1 cut(s) 484
BstH2I RGCGCY 2 cut(s) 464, 503
BstHHI GCGC 2 cut(s) 463, 502
BstKTI GATC 2 cut(s) 78, 373
BstMAI GTCTC 1 cut(s) 177
BstMBI GATC 2 cut(s) 75, 370
BstMWI GCNNNNNNNGC 1 cut(s) 323
BstSCI CCNGG 1 cut(s) 341
BstSFI CTRYAG 1 cut(s) 573
BstV1I GCAGC 3 cut(s) 71, 74, 326
BsuI GTATCC 1 cut(s) 547
BsuRI GGCC 1 cut(s) 295
BtgZI GCGATG 1 cut(s) 420
BtsCI GGATG 1 cut(s) 484
BtsIMutI CAGTG 3 cut(s) 211, 270, 296
Cac8I GCNNGC 6 cut(s) 47, 328, 332, 336, 366, 573
CaiI CAGNNNCTG 1 cut(s) 581
CfoI GCGC 2 cut(s) 463, 502
Cfr13I GGNCC 1 cut(s) 293
CviAII CATG 6 cut(s) 50, 179, 244, 253, 385, 449
DdeI CTNAG 1 cut(s) 203
DinI GGCGCC 2 cut(s) 462, 501
DpnI GATC 2 cut(s) 77, 372
DpnII GATC 2 cut(s) 75, 370
Eam1104I CTCTTC 3 cut(s) 210, 251, 561
EarI CTCTTC 3 cut(s) 210, 251, 561
Eco32I GATATC 1 cut(s) 226
EcoRV GATATC 1 cut(s) 226
EgeI GGCGCC 2 cut(s) 462, 501
EheI GGCGCC 2 cut(s) 462, 501
FaeI CATG 6 cut(s) 53, 182, 247, 256, 388, 452
FalI AAGNNNNNCTT 2 cut(s) 458, 490
FaqI GGGAC 2 cut(s) 65, 95
FatI CATG 6 cut(s) 49, 178, 243, 252, 384, 448
FauI CCCGC 1 cut(s) 357
FbaI TGATCA 1 cut(s) 370
FblI GTMKAC 1 cut(s) 138
Fnu4HI GCNGC 8 cut(s) 60, 63, 123, 312, 315, 362, 456, 459
FokI GGATG 1 cut(s) 491
Fsp4HI GCNGC 8 cut(s) 60, 63, 123, 312, 315, 362, 456, 459
FspBI CTAG 1 cut(s) 26
GlaI GCGC 2 cut(s) 462, 501
GluI GCNGC 8 cut(s) 60, 63, 123, 312, 315, 362, 456, 459
GsaI CCCAGC 1 cut(s) 321
HaeII RGCGCY 2 cut(s) 464, 503
HaeIII GGCC 1 cut(s) 295
HapII CCGG 2 cut(s) 342, 503
HhaI GCGC 2 cut(s) 463, 502
Hin1I GRCGYC 2 cut(s) 461, 500
Hin1II CATG 6 cut(s) 53, 182, 247, 256, 388, 452
Hin6I GCGC 2 cut(s) 461, 500
HinP1I GCGC 2 cut(s) 461, 500
HincII GTYRAC 1 cut(s) 139
HindII GTYRAC 1 cut(s) 139
HindIII AAGCTT 1 cut(s) 467
HpaII CCGG 2 cut(s) 342, 503
HphI GGTGA 2 cut(s) 101, 298
Hpy166II GTNNAC 3 cut(s) 139, 163, 238
Hpy8I GTNNAC 3 cut(s) 139, 163, 238
Hpy99I CGWCG 1 cut(s) 215
HpyAV CCTTC 1 cut(s) 395
HpyCH4III ACNGT 2 cut(s) 274, 300
HpyCH4IV ACGT 3 cut(s) 234, 240, 351
HpyCH4V TGCA 2 cut(s) 514, 575
HpyF10VI GCNNNNNNNGC 1 cut(s) 323
HpyF3I CTNAG 1 cut(s) 203
HpySE526I ACGT 3 cut(s) 234, 240, 351
Hsp92I GRCGYC 2 cut(s) 461, 500
Hsp92II CATG 6 cut(s) 53, 182, 247, 256, 388, 452
HspAI GCGC 2 cut(s) 461, 500
KasI GGCGCC 2 cut(s) 460, 499
Ksp22I TGATCA 1 cut(s) 370
Kzo9I GATC 2 cut(s) 75, 370
LguI GCTCTTC 1 cut(s) 561
LmnI GCTCC 1 cut(s) 445
LpnPI CCDG 6 cut(s) 303, 355, 502, 516, 536, 585
Lsp1109I GCAGC 3 cut(s) 71, 74, 326
LweI GCATC 1 cut(s) 208
MaeI CTAG 1 cut(s) 26
MaeII ACGT 3 cut(s) 234, 240, 351
MaeIII GTNAC 1 cut(s) 274
MalI GATC 2 cut(s) 77, 372
MboI GATC 2 cut(s) 75, 370
MboII GAAGA 8 cut(s) 145, 198, 201, 227, 268, 500, 578, 579
MluCI AATT 1 cut(s) 101
Mly113I GGCGCC 2 cut(s) 461, 500
MmeI TCCRAC 1 cut(s) 201
MnlI CCTC 5 cut(s) 167, 252, 314, 348, 445
MroXI GAANNNNTTC 1 cut(s) 193
MseI TTAA 2 cut(s) 423, 471
MslI CAYNNNNRTG 2 cut(s) 71, 204
MspA1I CMGCKG 2 cut(s) 284, 317
MspI CCGG 2 cut(s) 342, 503
MspR9I CCNGG 1 cut(s) 343
MwoI GCNNNNNNNGC 1 cut(s) 323
NarI GGCGCC 2 cut(s) 461, 500
NciI CCSGG 1 cut(s) 343
NdeII GATC 2 cut(s) 75, 370
NlaIII CATG 6 cut(s) 53, 182, 247, 256, 388, 452
NlaIV GGNNCC 3 cut(s) 294, 462, 501
NmeAIII GCCGAG 2 cut(s) 151, 476
NmuCI GTSAC 1 cut(s) 274
OliI CACNNNNGTG 1 cut(s) 71
PciSI GCTCTTC 1 cut(s) 561
PdmI GAANNNNTTC 1 cut(s) 193
PfoI TCCNGGA 1 cut(s) 341
PkrI GCNGC 8 cut(s) 61, 64, 124, 313, 316, 363, 457, 460
PluTI GGCGCC 2 cut(s) 464, 503
Psp1406I AACGTT 1 cut(s) 234
PspFI CCCAGC 1 cut(s) 317
PspN4I GGNNCC 3 cut(s) 294, 462, 501
PspPI GGNCC 1 cut(s) 293
PstI CTGCAG 1 cut(s) 577
PstNI CAGNNNCTG 1 cut(s) 581
PvuII CAGCTG 2 cut(s) 284, 317
RseI CAYNNNNRTG 2 cut(s) 71, 204
SalI GTCGAC 1 cut(s) 137
SapI GCTCTTC 1 cut(s) 561
SaqAI TTAA 2 cut(s) 423, 471
SatI GCNGC 8 cut(s) 60, 63, 123, 312, 315, 362, 456, 459
Sau3AI GATC 2 cut(s) 75, 370
Sau96I GGNCC 1 cut(s) 293
ScrFI CCNGG 1 cut(s) 343
SfaNI GCATC 1 cut(s) 208
SfcI CTRYAG 1 cut(s) 573
SfoI GGCGCC 2 cut(s) 462, 501
SmiMI CAYNNNNRTG 2 cut(s) 71, 204
Sse9I AATT 1 cut(s) 101
SsiI CCGC 8 cut(s) 87, 123, 309, 312, 361, 364, 455, 458
SspDI GGCGCC 2 cut(s) 460, 499
SspI AATATT 1 cut(s) 39
SspMI CTAG 1 cut(s) 26
StyD4I CCNGG 1 cut(s) 341
TaaI ACNGT 2 cut(s) 274, 300
TaiI ACGT 3 cut(s) 237, 243, 354
TaqI TCGA 2 cut(s) 138, 486
TasI AATT 1 cut(s) 101
TauI GCSGC 5 cut(s) 125, 314, 364, 458, 461
Tru1I TTAA 2 cut(s) 423, 471
Tru9I TTAA 2 cut(s) 423, 471
TscAI CASTG 3 cut(s) 211, 277, 303
TseFI GTSAC 1 cut(s) 274
TseI GCWGC 3 cut(s) 59, 62, 314
Tsp45I GTSAC 1 cut(s) 274
TspDTI ATGAA 3 cut(s) 269, 401, 414
TspRI CASTG 3 cut(s) 211, 277, 303
XmiI GTMKAC 1 cut(s) 138
XmnI GAANNNNTTC 1 cut(s) 193
XspI CTAG 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.