AT3G03280

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0003619
Physical Location & Seq
Forward (+)
765420 .. 766387
968 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G03280.1

Sequence Viewer

Length: 501 bp
ATGGGCAACTACGTTTCATGCGCTCTAAACAAAACGTCATCGTCACCGTTGGCAAAAGTGATTCTCCCTGACGGTGGAGTGCGTGACATCCACGTGCCGACGAAAGCAGCCGAGCTCATGATGGAGATGCCAAGCTATTTCCTCGTCGACACCAAGTCGGTGAAAGTAGGCCGTAAATTTATCCCACTCGCCGCCGACGACGATCTTGATCTTGGAGGCTGTCATGTGTACGTCGCATTTCCGATGACACGTGCCACTTCTGCAGCCAACGCTTCCGACATGGCTAGGCTCTACCTCACCGGAAAGAAACGCACGAAAAACTGCGGTCATAGGAGAGTGTCTCCGGAAAATGAAGATAATGATGACGTGAGGCTGATCGGACCGAAGCTGAATCTTGAAGACATAGAAGAGTTCTCGGCGGCAGAGTTTATTCATCGGATCTCTGTTTCGAAATCTAAGAAACCACAGTTGGAGACGATAGCTGAAGATCATGTGCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

166

Amino Acids

18.24

Weight (kDa)

6.51

Isoelectric Point (pI)

43.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 162 1.1e-30 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 147
AccIII TCCGGA 1 cut(s) 343
AciI CCGC 3 cut(s) 192, 324, 419
AclWI GGATC 1 cut(s) 446
AcsI RAATTY 1 cut(s) 176
AcvI CACGTG 2 cut(s) 94, 251
AfaI GTAC 1 cut(s) 230
AfiI CCNNNNNNNGG 1 cut(s) 74
AflIII ACRYGT 1 cut(s) 248
AgsI TTSAA 1 cut(s) 398
AhdI GACNNNNNGTC 1 cut(s) 154
AjiI CACGTC 1 cut(s) 367
AjuI GAANNNNNNNTTGG 2 cut(s) 452, 484
AluBI AGCT 4 cut(s) 115, 135, 388, 482
AluI AGCT 4 cut(s) 115, 135, 388, 482
Alw21I GWGCWC 1 cut(s) 117
Alw26I GTCTC 2 cut(s) 345, 467
AlwI GGATC 1 cut(s) 446
Aor13HI TCCGGA 1 cut(s) 343
AoxI GGCC 1 cut(s) 169
ApeKI GCWGC 2 cut(s) 107, 263
ApoI RAATTY 1 cut(s) 176
AspLEI GCGC 1 cut(s) 23
AspS9I GGNCC 1 cut(s) 380
AsuHPI GGTGA 3 cut(s) 36, 172, 289
AsuII TTCGAA 1 cut(s) 449
AvaII GGWCC 1 cut(s) 380
BaeGI GKGCMC 1 cut(s) 498
BanII GRGCYC 1 cut(s) 117
BbrPI CACGTG 2 cut(s) 94, 251
BbsI GAAGAC 1 cut(s) 405
Bbv12I GWGCWC 1 cut(s) 117
BbvI GCAGC 2 cut(s) 119, 275
BccI CCATC 1 cut(s) 115
BceAI ACGGC 1 cut(s) 156
BcoDI GTCTC 2 cut(s) 345, 467
BfaI CTAG 1 cut(s) 285
BfmI CTRYAG 1 cut(s) 261
BisI GCNGC 4 cut(s) 108, 192, 264, 420
BlsI GCNGC 4 cut(s) 109, 193, 265, 421
Bme18I GGWCC 1 cut(s) 380
BmeRI GACNNNNNGTC 1 cut(s) 154
BmgBI CACGTC 1 cut(s) 367
BmgT120I GGNCC 1 cut(s) 380
BmsI GCATC 1 cut(s) 117
BpiI GAAGAC 1 cut(s) 405
BplI GAGNNNNNCTC 2 cut(s) 325, 357
Bpu14I TTCGAA 1 cut(s) 449
BsaAI YACGTR 2 cut(s) 94, 251
BsaBI GATNNNNATC 1 cut(s) 207
BsaWI WCCGGW 2 cut(s) 299, 343
Bsc4I CCNNNNNNNGG 1 cut(s) 74
Bse8I GATNNNNATC 1 cut(s) 207
BseAI TCCGGA 1 cut(s) 343
BseGI GGATG 1 cut(s) 87
BseJI GATNNNNATC 1 cut(s) 207
BseLI CCNNNNNNNGG 1 cut(s) 74
BseSI GKGCMC 1 cut(s) 498
BseXI GCAGC 2 cut(s) 119, 275
BshFI GGCC 1 cut(s) 171
BsiHKAI GWGCWC 1 cut(s) 117
BsiSI CCGG 2 cut(s) 300, 344
BslI CCNNNNNNNGG 1 cut(s) 74
BsmAI GTCTC 2 cut(s) 345, 467
BsmBI CGTCTC 1 cut(s) 467
BsnI GGCC 1 cut(s) 171
Bsp119I TTCGAA 1 cut(s) 449
Bsp1286I GDGCHC 2 cut(s) 117, 498
Bsp13I TCCGGA 1 cut(s) 343
Bsp143I GATC 5 cut(s) 202, 208, 375, 438, 487
BspACI CCGC 3 cut(s) 192, 324, 419
BspANI GGCC 1 cut(s) 171
BspEI TCCGGA 1 cut(s) 343
BspHI TCATGA 1 cut(s) 117
BspMAI CTGCAG 1 cut(s) 265
BspPI GGATC 1 cut(s) 446
BspT104I TTCGAA 1 cut(s) 449
BssMI GATC 5 cut(s) 202, 208, 375, 438, 487
Bst4CI ACNGT 3 cut(s) 48, 74, 468
Bst6I CTCTTC 1 cut(s) 402
BstBAI YACGTR 2 cut(s) 94, 251
BstBI TTCGAA 1 cut(s) 449
BstDEI CTNAG 1 cut(s) 456
BstF5I GGATG 1 cut(s) 87
BstHHI GCGC 1 cut(s) 23
BstKTI GATC 5 cut(s) 205, 211, 378, 441, 490
BstMAI GTCTC 2 cut(s) 345, 467
BstMBI GATC 5 cut(s) 202, 208, 375, 438, 487
BstMWI GCNNNNNNNGC 2 cut(s) 260, 269
BstSFI CTRYAG 1 cut(s) 261
BstSLI GKGCMC 1 cut(s) 498
BstV1I GCAGC 2 cut(s) 119, 275
BstV2I GAAGAC 1 cut(s) 405
BstX2I RGATCY 1 cut(s) 438
BstYI RGATCY 1 cut(s) 438
BsuRI GGCC 1 cut(s) 171
BtrI CACGTC 1 cut(s) 367
BtsCI GGATG 1 cut(s) 87
CciI TCATGA 1 cut(s) 117
CfoI GCGC 1 cut(s) 23
Cfr13I GGNCC 1 cut(s) 380
CpoI CGGWCCG 1 cut(s) 380
Csp6I GTAC 1 cut(s) 229
CspI CGGWCCG 1 cut(s) 380
CviAII CATG 5 cut(s) 18, 118, 224, 280, 491
CviQI GTAC 1 cut(s) 229
DdeI CTNAG 1 cut(s) 456
DpnI GATC 5 cut(s) 204, 210, 377, 440, 489
DpnII GATC 5 cut(s) 202, 208, 375, 438, 487
DriI GACNNNNNGTC 1 cut(s) 154
Eam1104I CTCTTC 1 cut(s) 402
Eam1105I GACNNNNNGTC 1 cut(s) 154
EarI CTCTTC 1 cut(s) 402
Ecl136II GAGCTC 1 cut(s) 115
Eco24I GRGCYC 1 cut(s) 117
Eco47I GGWCC 1 cut(s) 380
Eco53kI GAGCTC 1 cut(s) 115
Eco72I CACGTG 2 cut(s) 94, 251
EcoICRI GAGCTC 1 cut(s) 115
EcoT38I GRGCYC 1 cut(s) 117
Esp3I CGTCTC 1 cut(s) 467
FaeI CATG 5 cut(s) 21, 121, 227, 283, 494
FaiI YATR 7 cut(s) 19, 119, 225, 281, 330, 404, 492
FatI CATG 5 cut(s) 17, 117, 223, 279, 490
FblI GTMKAC 1 cut(s) 147
Fnu4HI GCNGC 4 cut(s) 108, 192, 264, 420
FokI GGATG 1 cut(s) 74
FriOI GRGCYC 1 cut(s) 117
Fsp4HI GCNGC 4 cut(s) 108, 192, 264, 420
FspBI CTAG 1 cut(s) 285
GlaI GCGC 1 cut(s) 22
GluI GCNGC 4 cut(s) 108, 192, 264, 420
HaeIII GGCC 1 cut(s) 171
HapII CCGG 2 cut(s) 300, 344
HhaI GCGC 1 cut(s) 23
Hin1II CATG 5 cut(s) 21, 121, 227, 283, 494
Hin6I GCGC 1 cut(s) 21
HinP1I GCGC 1 cut(s) 21
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HinfI GANTC 2 cut(s) 61, 391
HpaII CCGG 2 cut(s) 300, 344
HphI GGTGA 3 cut(s) 36, 172, 289
Hpy166II GTNNAC 2 cut(s) 148, 229
Hpy188I TCNGA 4 cut(s) 243, 277, 380, 438
Hpy188III TCNNGA 4 cut(s) 118, 206, 344, 395
Hpy8I GTNNAC 2 cut(s) 148, 229
Hpy99I CGWCG 5 cut(s) 103, 149, 200, 203, 236
HpyCH4III ACNGT 3 cut(s) 48, 74, 468
HpyCH4IV ACGT 6 cut(s) 12, 35, 93, 231, 250, 366
HpyCH4V TGCA 1 cut(s) 263
HpyF10VI GCNNNNNNNGC 2 cut(s) 260, 269
HpyF3I CTNAG 1 cut(s) 456
HpySE526I ACGT 6 cut(s) 12, 35, 93, 231, 250, 366
Hsp92II CATG 5 cut(s) 21, 121, 227, 283, 494
HspAI GCGC 1 cut(s) 21
Kpn2I TCCGGA 1 cut(s) 343
Kzo9I GATC 5 cut(s) 202, 208, 375, 438, 487
LpnPI CCDG 3 cut(s) 81, 313, 357
Lsp1109I GCAGC 2 cut(s) 119, 275
LweI GCATC 1 cut(s) 117
MaeI CTAG 1 cut(s) 285
MaeII ACGT 6 cut(s) 12, 35, 93, 231, 250, 366
MaeIII GTNAC 2 cut(s) 42, 83
MalI GATC 5 cut(s) 204, 210, 377, 440, 489
MboI GATC 5 cut(s) 202, 208, 375, 438, 487
MboII GAAGA 4 cut(s) 365, 410, 419, 497
MflI RGATCY 1 cut(s) 438
MhlI GDGCHC 2 cut(s) 117, 498
MluCI AATT 1 cut(s) 176
MmeI TCCRAC 2 cut(s) 300, 450
MnlI CCTC 4 cut(s) 152, 209, 305, 363
MroI TCCGGA 1 cut(s) 343
MslI CAYNNNNRTG 1 cut(s) 92
MspI CCGG 2 cut(s) 300, 344
MwoI GCNNNNNNNGC 2 cut(s) 260, 269
NdeII GATC 5 cut(s) 202, 208, 375, 438, 487
NlaIII CATG 5 cut(s) 21, 121, 227, 283, 494
NmeAIII GCCGAG 2 cut(s) 136, 395
NmuCI GTSAC 2 cut(s) 42, 83
NspV TTCGAA 1 cut(s) 449
PagI TCATGA 1 cut(s) 117
PcsI WCGNNNNNNNCGW 1 cut(s) 195
PfeI GAWTC 2 cut(s) 61, 391
PkrI GCNGC 4 cut(s) 109, 193, 265, 421
PmaCI CACGTG 2 cut(s) 94, 251
PmlI CACGTG 2 cut(s) 94, 251
Ppu21I YACGTR 2 cut(s) 94, 251
Psp124BI GAGCTC 1 cut(s) 117
PspCI CACGTG 2 cut(s) 94, 251
PspPI GGNCC 1 cut(s) 380
PstI CTGCAG 1 cut(s) 265
PsuI RGATCY 1 cut(s) 438
RsaI GTAC 1 cut(s) 230
RsaNI GTAC 1 cut(s) 229
RseI CAYNNNNRTG 1 cut(s) 92
Rsr2I CGGWCCG 1 cut(s) 380
RsrII CGGWCCG 1 cut(s) 380
SacI GAGCTC 1 cut(s) 117
SalI GTCGAC 1 cut(s) 146
SatI GCNGC 4 cut(s) 108, 192, 264, 420
Sau3AI GATC 5 cut(s) 202, 208, 375, 438, 487
Sau96I GGNCC 1 cut(s) 380
SduI GDGCHC 2 cut(s) 117, 498
SfaNI GCATC 1 cut(s) 117
SfcI CTRYAG 1 cut(s) 261
SfuI TTCGAA 1 cut(s) 449
SinI GGWCC 1 cut(s) 380
SmiMI CAYNNNNRTG 1 cut(s) 92
Sse9I AATT 1 cut(s) 176
SsiI CCGC 3 cut(s) 192, 324, 419
SspMI CTAG 1 cut(s) 285
SstI GAGCTC 1 cut(s) 117
TaaI ACNGT 3 cut(s) 48, 74, 468
TaiI ACGT 6 cut(s) 15, 38, 96, 234, 253, 369
TaqI TCGA 2 cut(s) 147, 449
TaqII GACCGA 1 cut(s) 397
TasI AATT 1 cut(s) 176
TauI GCSGC 2 cut(s) 194, 422
TfiI GAWTC 2 cut(s) 61, 391
TseFI GTSAC 2 cut(s) 42, 83
TseI GCWGC 2 cut(s) 107, 263
Tsp45I GTSAC 2 cut(s) 42, 83
TspDTI ATGAA 3 cut(s) 6, 366, 422
VpaK11BI GGWCC 1 cut(s) 380
XapI RAATTY 1 cut(s) 176
XmiI GTMKAC 1 cut(s) 147
XspI CTAG 1 cut(s) 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.