pycom09g17340

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0003619
Physical Location & Seq
Reverse (-)
17814495 .. 17815259
765 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g17340.1

Sequence Viewer

Length: 765 bp
ATGCTTTTATTCATATTTTTAGTTGAGAGACGTCTTCATCAATTCCCTTGTATAAATCTTCAACTCCCTCAAACTTCCAAGACCAAAAGTTTCATCTCATTTCGTATACCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCGGCAACATGGGCAACTACATTTCTTGTAGCCTAGCAACCCAAATATTGAGCAAGCATGGAAAAGCAGCAACCACTAAAGTGATCGTCCCAAGCGGTGAAATCAAACAATTCAATGTCCCCATAAAAGCCGCCGAGCTGATGGTCGACACCCCAAACTTCTTCCTCGTAAATATCCAGTCTCTCCATGTGGGAAGAAGATTTTCGCATCTCAGTGCCGACGAAGAGTTGGATATCGGCAACGTTTACGTCATGTTCCCCATGAAGAGGCTCAACTCCACCGTCACAACAGCTGATATGGGGCCACTGTTCCACACCGCCGCAGCTGGGTCAGCAAGCAAGCGAGCTTCCGGGAGAAACGTGAGGGTGCGGCCGGCTTATCATCATCAGCCCATGAAGTTTGGTAATGAAGGCGATGAGTGTAAGAGGATTAAGAGTTGGGGAAAAAGTTTGGAGCATGAGGCGGCGACGCCAAAGCTTAACTTAGATGACATCGAAGATTTCTCGGCGCCGGAGTTTATGCACAGGTTGTCTATGAGTAGGTCAAAGAAGCCATTGCTGGATACCATAGCAGAAGAGCCTGCAGTTTCTGTTTCTTCAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

255

Amino Acids

28.19

Weight (kDa)

9.51

Isoelectric Point (pI)

40.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 57 - 245 1.8e-35 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 34
AccB1I GGYRCC 1 cut(s) 667
AccI GTMKAC 2 cut(s) 106, 306
AciI CCGC 6 cut(s) 255, 291, 477, 480, 529, 623
AclI AACGTT 1 cut(s) 402
AcoI YGGCCR 1 cut(s) 530
AcyI GRCGYC 3 cut(s) 31, 629, 668
AfiI CCNNNNNNNGG 2 cut(s) 426, 486
AgsI TTSAA 3 cut(s) 62, 274, 759
AleI CACNNNNGTG 1 cut(s) 239
AluBI AGCT 5 cut(s) 298, 452, 485, 506, 637
AluI AGCT 5 cut(s) 298, 452, 485, 506, 637
Alw26I GTCTC 2 cut(s) 22, 345
AlwNI CAGNNNCTG 1 cut(s) 749
AoxI GGCC 2 cut(s) 461, 530
ApeKI GCWGC 2 cut(s) 227, 482
Asp700I GAANNNNTTC 1 cut(s) 361
AspLEI GCGC 1 cut(s) 670
AspS9I GGNCC 1 cut(s) 461
AsuC2I CCSGG 1 cut(s) 511
AsuHPI GGTGA 1 cut(s) 269
BanI GGYRCC 1 cut(s) 667
BbsI GAAGAC 1 cut(s) 26
BbvI GCAGC 2 cut(s) 239, 494
BccI CCATC 1 cut(s) 295
BciVI GTATCC 1 cut(s) 715
BcnI CCSGG 1 cut(s) 511
BcoDI GTCTC 2 cut(s) 22, 345
BfaI CTAG 1 cut(s) 194
BfmI CTRYAG 1 cut(s) 741
BfoI RGCGCY 1 cut(s) 671
BfuI GTATCC 1 cut(s) 715
BisI GCNGC 6 cut(s) 228, 291, 480, 483, 530, 624
BlsI GCNGC 6 cut(s) 229, 292, 481, 484, 531, 625
Bme1390I CCNGG 1 cut(s) 511
BmgT120I GGNCC 1 cut(s) 461
BmiI GGNNCC 2 cut(s) 462, 669
BmrFI CCNGG 1 cut(s) 511
BmsI GCATC 1 cut(s) 376
BpiI GAAGAC 1 cut(s) 26
BpuMI CCSGG 1 cut(s) 511
BsaHI GRCGYC 3 cut(s) 31, 629, 668
Bsc4I CCNNNNNNNGG 2 cut(s) 426, 486
Bse118I RCCGGY 1 cut(s) 532
Bse1I ACTGG 1 cut(s) 337
Bse3DI GCAATG 1 cut(s) 713
BseLI CCNNNNNNNGG 2 cut(s) 426, 486
BseMI GCAATG 1 cut(s) 713
BseMII CTCAG 1 cut(s) 385
BseNI ACTGG 1 cut(s) 337
BseX3I CGGCCG 1 cut(s) 530
BseXI GCAGC 2 cut(s) 239, 494
BseYI CCCAGC 1 cut(s) 485
Bsh1285I CGRYCG 1 cut(s) 533
BshFI GGCC 2 cut(s) 463, 532
BshNI GGYRCC 1 cut(s) 667
BsiEI CGRYCG 1 cut(s) 533
BsiSI CCGG 3 cut(s) 510, 533, 671
BslFI GGGAC 2 cut(s) 233, 263
BslI CCNNNNNNNGG 2 cut(s) 426, 486
BsmAI GTCTC 2 cut(s) 22, 345
BsmBI CGTCTC 1 cut(s) 22
BsmFI GGGAC 2 cut(s) 233, 263
BsnI GGCC 2 cut(s) 463, 532
Bsp143I GATC 1 cut(s) 243
BspACI CCGC 6 cut(s) 255, 291, 477, 480, 529, 623
BspANI GGCC 2 cut(s) 463, 532
BspCNI CTCAG 1 cut(s) 384
BspLI GGNNCC 2 cut(s) 462, 669
BspMAI CTGCAG 1 cut(s) 745
BspQI GCTCTTC 1 cut(s) 729
BspT107I GGYRCC 1 cut(s) 667
BsrDI GCAATG 1 cut(s) 713
BsrFI RCCGGY 1 cut(s) 532
BsrI ACTGG 1 cut(s) 337
BssAI RCCGGY 1 cut(s) 532
BssMI GATC 1 cut(s) 243
BssNAI GTATAC 1 cut(s) 107
BssNI GRCGYC 3 cut(s) 31, 629, 668
Bst1107I GTATAC 1 cut(s) 107
Bst4CI ACNGT 2 cut(s) 442, 468
Bst6I CTCTTC 3 cut(s) 378, 419, 729
BstACI GRCGYC 3 cut(s) 31, 629, 668
BstC8I GCNNGC 6 cut(s) 215, 496, 500, 504, 534, 741
BstDEI CTNAG 2 cut(s) 371, 643
BstH2I RGCGCY 1 cut(s) 671
BstHHI GCGC 1 cut(s) 670
BstKTI GATC 1 cut(s) 246
BstMAI GTCTC 2 cut(s) 22, 345
BstMBI GATC 1 cut(s) 243
BstMCI CGRYCG 1 cut(s) 533
BstMWI GCNNNNNNNGC 2 cut(s) 171, 491
BstSCI CCNGG 1 cut(s) 509
BstSFI CTRYAG 1 cut(s) 741
BstV1I GCAGC 2 cut(s) 239, 494
BstV2I GAAGAC 1 cut(s) 26
BstZ17I GTATAC 1 cut(s) 107
BstZI CGGCCG 1 cut(s) 530
BsuI GTATCC 1 cut(s) 715
BsuRI GGCC 2 cut(s) 463, 532
BtgZI GCGATG 1 cut(s) 588
BtsIMutI CAGTG 2 cut(s) 379, 464
Cac8I GCNNGC 6 cut(s) 215, 496, 500, 504, 534, 741
CaiI CAGNNNCTG 1 cut(s) 749
CfoI GCGC 1 cut(s) 670
Cfr10I RCCGGY 1 cut(s) 532
Cfr13I GGNCC 1 cut(s) 461
CseI GACGC 1 cut(s) 637
CviAII CATG 7 cut(s) 169, 218, 347, 412, 421, 553, 617
DdeI CTNAG 2 cut(s) 371, 643
DinI GGCGCC 1 cut(s) 669
DpnI GATC 1 cut(s) 245
DpnII GATC 1 cut(s) 243
EaeI YGGCCR 1 cut(s) 530
EagI CGGCCG 1 cut(s) 530
Eam1104I CTCTTC 3 cut(s) 378, 419, 729
EarI CTCTTC 3 cut(s) 378, 419, 729
EclXI CGGCCG 1 cut(s) 530
Eco32I GATATC 1 cut(s) 394
Eco52I CGGCCG 1 cut(s) 530
EcoRV GATATC 1 cut(s) 394
EgeI GGCGCC 1 cut(s) 669
EheI GGCGCC 1 cut(s) 669
Esp3I CGTCTC 1 cut(s) 22
FaeI CATG 7 cut(s) 172, 221, 350, 415, 424, 556, 620
FalI AAGNNNNNCTT 2 cut(s) 626, 658
FaqI GGGAC 2 cut(s) 233, 263
FatI CATG 7 cut(s) 168, 217, 346, 411, 420, 552, 616
FblI GTMKAC 2 cut(s) 106, 306
Fnu4HI GCNGC 6 cut(s) 228, 291, 480, 483, 530, 624
Fsp4HI GCNGC 6 cut(s) 228, 291, 480, 483, 530, 624
FspBI CTAG 1 cut(s) 194
GlaI GCGC 1 cut(s) 669
GluI GCNGC 6 cut(s) 228, 291, 480, 483, 530, 624
GsaI CCCAGC 1 cut(s) 489
HaeII RGCGCY 1 cut(s) 671
HaeIII GGCC 2 cut(s) 463, 532
HapII CCGG 3 cut(s) 510, 533, 671
HgaI GACGC 1 cut(s) 637
HhaI GCGC 1 cut(s) 670
Hin1I GRCGYC 3 cut(s) 31, 629, 668
Hin1II CATG 7 cut(s) 172, 221, 350, 415, 424, 556, 620
Hin6I GCGC 1 cut(s) 668
HinP1I GCGC 1 cut(s) 668
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
HindIII AAGCTT 1 cut(s) 635
HpaII CCGG 3 cut(s) 510, 533, 671
HphI GGTGA 1 cut(s) 269
Hpy166II GTNNAC 3 cut(s) 107, 307, 406
Hpy8I GTNNAC 3 cut(s) 107, 307, 406
Hpy99I CGWCG 2 cut(s) 383, 631
HpyAV CCTTC 1 cut(s) 563
HpyCH4III ACNGT 2 cut(s) 442, 468
HpyCH4IV ACGT 4 cut(s) 31, 402, 408, 519
HpyCH4V TGCA 2 cut(s) 682, 743
HpyF10VI GCNNNNNNNGC 2 cut(s) 171, 491
HpyF3I CTNAG 2 cut(s) 371, 643
HpySE526I ACGT 4 cut(s) 31, 402, 408, 519
Hsp92I GRCGYC 3 cut(s) 31, 629, 668
Hsp92II CATG 7 cut(s) 172, 221, 350, 415, 424, 556, 620
HspAI GCGC 1 cut(s) 668
KasI GGCGCC 1 cut(s) 667
KroI GCCGGC 1 cut(s) 532
KroNI GCCGGC 1 cut(s) 534
Kzo9I GATC 1 cut(s) 243
LguI GCTCTTC 1 cut(s) 729
LmnI GCTCC 1 cut(s) 613
LpnPI CCDG 8 cut(s) 350, 471, 523, 546, 670, 684, 704, 753
Lsp1109I GCAGC 2 cut(s) 239, 494
LweI GCATC 1 cut(s) 376
MaeI CTAG 1 cut(s) 194
MaeII ACGT 4 cut(s) 31, 402, 408, 519
MaeIII GTNAC 1 cut(s) 442
MalI GATC 1 cut(s) 245
MboI GATC 1 cut(s) 243
MluCI AATT 2 cut(s) 41, 269
Mly113I GGCGCC 1 cut(s) 668
MmeI TCCRAC 1 cut(s) 369
MnlI CCTC 7 cut(s) 78, 120, 335, 420, 516, 579, 613
MroNI GCCGGC 1 cut(s) 532
MroXI GAANNNNTTC 1 cut(s) 361
MseI TTAA 2 cut(s) 591, 639
MslI CAYNNNNRTG 2 cut(s) 239, 372
MspA1I CMGCKG 2 cut(s) 452, 485
MspI CCGG 3 cut(s) 510, 533, 671
MspR9I CCNGG 1 cut(s) 511
MwoI GCNNNNNNNGC 2 cut(s) 171, 491
NaeI GCCGGC 1 cut(s) 534
NarI GGCGCC 1 cut(s) 668
NciI CCSGG 1 cut(s) 511
NdeII GATC 1 cut(s) 243
NgoMIV GCCGGC 1 cut(s) 532
NlaIII CATG 7 cut(s) 172, 221, 350, 415, 424, 556, 620
NlaIV GGNNCC 2 cut(s) 462, 669
NmeAIII GCCGAG 3 cut(s) 141, 319, 644
NmuCI GTSAC 1 cut(s) 442
OliI CACNNNNGTG 1 cut(s) 239
PciSI GCTCTTC 1 cut(s) 729
PdiI GCCGGC 1 cut(s) 534
PdmI GAANNNNTTC 1 cut(s) 361
PfoI TCCNGGA 1 cut(s) 509
PkrI GCNGC 6 cut(s) 229, 292, 481, 484, 531, 625
PluTI GGCGCC 1 cut(s) 671
Psp1406I AACGTT 1 cut(s) 402
PspFI CCCAGC 1 cut(s) 485
PspN4I GGNNCC 2 cut(s) 462, 669
PspPI GGNCC 1 cut(s) 461
PstI CTGCAG 1 cut(s) 745
PstNI CAGNNNCTG 1 cut(s) 749
PvuII CAGCTG 2 cut(s) 452, 485
RseI CAYNNNNRTG 2 cut(s) 239, 372
SalI GTCGAC 1 cut(s) 305
SapI GCTCTTC 1 cut(s) 729
SaqAI TTAA 2 cut(s) 591, 639
SatI GCNGC 6 cut(s) 228, 291, 480, 483, 530, 624
Sau3AI GATC 1 cut(s) 243
Sau96I GGNCC 1 cut(s) 461
ScrFI CCNGG 1 cut(s) 511
SfaNI GCATC 1 cut(s) 376
SfcI CTRYAG 1 cut(s) 741
SfoI GGCGCC 1 cut(s) 669
SmiMI CAYNNNNRTG 2 cut(s) 239, 372
Sse9I AATT 2 cut(s) 41, 269
SsiI CCGC 6 cut(s) 255, 291, 477, 480, 529, 623
SspDI GGCGCC 1 cut(s) 667
SspI AATATT 1 cut(s) 207
SspMI CTAG 1 cut(s) 194
StyD4I CCNGG 1 cut(s) 509
TaaI ACNGT 2 cut(s) 442, 468
TaiI ACGT 4 cut(s) 34, 405, 411, 522
TaqI TCGA 2 cut(s) 306, 654
TasI AATT 2 cut(s) 41, 269
TauI GCSGC 4 cut(s) 293, 482, 532, 626
Tru1I TTAA 2 cut(s) 591, 639
Tru9I TTAA 2 cut(s) 591, 639
TscAI CASTG 2 cut(s) 379, 471
TseFI GTSAC 1 cut(s) 442
TseI GCWGC 2 cut(s) 227, 482
Tsp45I GTSAC 1 cut(s) 442
TspDTI ATGAA 5 cut(s) 26, 82, 437, 569, 582
TspRI CASTG 2 cut(s) 379, 471
XmiI GTMKAC 2 cut(s) 106, 306
XmnI GAANNNNTTC 1 cut(s) 361
XspI CTAG 1 cut(s) 194
ZraI GACGTC 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.