MD00G1054100.v1.1

DNA ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0015031
Physical Location & Seq
Forward (+)
10093090 .. 10111659
18570 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1054100.v1.1.491

Sequence Viewer

Length: 4245 bp
ATGGCCTCCTCCTCCTCTTCCGTGACCAGAACCCCAACCCTTGATTCCACCCACCTCTTCCTCGCCGCCCTCGCCGCCTTCTCCCACCAACCCAACCCTCCCCATCCCTCTTTCCCTCCAATTCCCTCCACATTCCCTCACTCCAAACTCATCCCCAAAACCCGTTTCGCCGTCGACGCATTCCGCCACGCCGGCGATCACTCCGTCTCCTACTTCCTCTCCCATTTCCACTCCGATCACTACGGCGGCCTCTCCCCCAATTGGGCTAAAGGCGTCGTCTTTTGTTCTCCGATCACCGCCCGTCTTCTCACTGAGGTGCTCAAGGTCTCTCCTCATTTCGTTGTCGCTCTGCCGCTGGGCGAAGCCGTTGTAATCGATGGGGTGAAGGTTGTGCTGATTGACGCTAATCACTGTCCGGGTGCGGTTCAGTTCTTGTTTAAAATTCCGGATATGAACGGAAAGTTTGAGAGGTATGTTCATACTGGCGATTTTAGGTTTTCGGGTTCAATGAAGTTAGACCCTTTTCTGTGTGAATTTGTGGGTTCCGATGCTGTTTTCTTGGACACTACGTATTGTAATCCGAAATTTGTGTTTCCTTCACAACAAGAGTCTGTAGATTATATTGCTAGTGTGATAGAACGAGTTGGGGGTGAATGTAAAAGTGAAATGAAAAATGTTTTGTTTCTTGTAGCTACTTATGTAATTGGAAAAGAGAAGATTTTGCTTGAAATTGCGCATAGGTGTAATCGTAAGGTGCATGTGGATGCTCGTAAAATGGCAGTTTTTCGTGTTTTGGGTTATGGAGAGAATGGGGTATTTACAGAGGATGAATGTGAAAGTGATGTACATGTTGTTGGGTGGAATGTCTTGGGTGAGACTTGGCCATATTTTAAGCCTAATTTTGTGAAAATGAAGGAGATTATGGCCGAAAGAGGATACTCTAAGGTTGTGGGTTTTGTTCCTACTGGTTGGACTTATGAAGTGAAACGTAATAAGTTTTCGGTTAGGTCGAAGGATTCGTTTGAGATCCATCTTGTACCATATAGTGAACATTCAAACTATGAAGAACTTAGAGAATATGTGAGGTTTTTGAAACCAAAGCGTGTTGTTCCTACAGTGGGCTTGGATGTTGAAAAGCTTGATAGTAAGCACGCCAGTAAAATGCAAAAGCATTTTGCTGGGTTAGTTGATGAAATGGCAAACAAGAAGGAGTTCTTGAAGAGCTTTCGTCTTGGGTCTAAAGAAGTAGCTGACAGTGTAGAAAATGAGTCCAACAAGGGCCCCATTTTGGGGCTCGGTGAGGAGAAAGAGGCACTGTCATATGATAAGGATATAGTAGGAAATAGTATTATCAGTTTAGGCATTGAATTATCATCTTCTCTACAAGAACCTGATCGACAAAATCAAGTGGTGTTAAATGATGAGGAAGCAGAAAAAATCGTCCAAGAACTTCGTGATTGTTTGCCCACATGGGTCACCCATGAGCAGATGCTAGTTTTGATTGGTAGCACGGGAGGGGACATCGTTGAAGCAGTTTCTAATTTCTACGAACGCGAGACAGAATTTCATGAGCAAGTAAATGCTTCTAGAATTGCTGATTCTGTGTCCCAGACTACCTCAACTTGTGACTCTGCATCACTTCCAAAAGCAGGCTCTGATAAGAGCAGTCCATGTGGAAGTACTGACATTCCTTTAAGTCAAGATTATATCTCACTTATGCCAAGGAATATGATTAAAAGTGGAATATCTCCTGGGAAAAGGGCGAGTAAAGTTAACAATAAGGTAAACAAAAAGTTGAAATTAAATTCAAAATTGGATTCAGGCGGGCCGAAGCAACTGGCAATAACAAGGTTTTTTAGTAAAGTCTTACCAAATGTTCCAGAAACTGCGGTGGCTGGTTCATTGCATGAACAAAACCCTAAATATGAAAATTTGCTCAACGATGACACCAAATCATACAGGGATGAGATACATCAGTTTCTTCAGATAATAGATGGTGATGAATCGCTAGAGAGCTATGCTGCCACCATTCTGGAGGAGGCAAATGGAGATATCAATAAAGCGCTAAATATATATTACTCTAATCGTGAAGGAAGGTCCGGTGAGAATGAAGAAGAATTGGTGGTTAATGAAAAATCAGTTCAGTTCCACTGCCACATAAATAATTGCTCTTCCGACAAAGAGAAAACTCAGTCAAGGAAAATGGAACCAACAACTGATTCGTCTGTGCAGGTTTCATTACCAGTGAATGTAAATATAACTTTTGTGTCTCTACCACCAGAAAAATACAATCCTATAGAACATGCTTGCTGGAGGAATGGACAGAATGCTCCATATTTACATCTTGCAAGAACTTTTGACTTACTTGAGGATGAAAAGGGCAAGATAAAAGCTACTTCCATGCTCTGCAACATGTTCAGAAGTTTGCTTGCCTTGTCTCCTGATGACGTGCTGCCTTCAGTGTATTTGTGCACAAATAAGATCGCTGCTGACCATGAAAATGTGGAGCTAAACATCGGTGGAAGTTTGGTCACATCAGCACTGGAAGAAGCATGTGGGACAAGCCGATCCAAAATAAGGGAGATGTACAGTGAGCTGGGTGATCTAGGTGATGTTGCTCAAGCATGCCGGCAGACACAAAAGTTACTTGCCCCTCCTTCACCGTTGTTAATAAAGGATGTATTTTCTGCACTCCAAAAGATAAGTGTACAAACAGGTAGTGGAAGCACCGTTCGAAAGAAAAGCCTCATTTTGAATCTAATGCGCTCTTGTAGAGAGAAAGAGATGAAGTTTCTTGTTAGAACTTTGGTCAGGAATTTACGAATTGGAGCAATGATGAAAACTGTTCTACCTGCTCTGGCTCAAGCTGTTGTCATTAACTCCTCGTATAACTTCAATAATGAAGGAAGAGTAGAAAATATGAAAGATAAGCTTCAGCGTCATTCAGCTGCTGTAGTCGAAGCGTACAATGTTCTGCCCAATTTGGTAGATGCTGGTTTTGGTGATTTGGTCGTTCCTTCCCTCATGGACAAGGGCGTTGGATTTTCTTCGTCAACCTTGGCAATGGTTCCAGGAATACCTATTAAGCCAATGCTTGCAAAGATCACGAATGGTGTTCAGCAAGCACTGAAGCTCTTTGAAAATAAAGCTTTTACATGTGAATACAAATATGATGGTCAGCGTGCCCAAATTCACAAATTGGTTGATGGGTCTCTGCGCGTTTTTTCACGAAATGGGGACGAGTCAACATCAAGATTTCCAGATCTGATTAACATAGTTAATGCATCTTGTAAGCCTGATGCAGAAACTTTTATACTAGATGCGGAGGTAGTTGCAGTCGATAGGAATAATGGTCACAAGCTAATGTCTTTTCAAGAATTGTCGTCACGTGGGAGAGGGGGCAGAGATACATCAATTACATTGGATAGCATAAAGGTTGTCGACATCTGCGTATTTGTCTTTGATATCATGTTTGCCAATGGAAAGCAGTTGCTGGGCTTTCCTCTGCGGCAAAGGCGAACATATTTGAAAGATATGTTTTATGTTGAGAAGCCGGGTTATTTCGAATATGCCAAGGAAATTACAGTTGAAGCAGATGATGCTTGTTTAACCAGTGAAGCCACGTTAGCAAAGATCAGCTGCTTTCTTGAAAATTCATTCCAATCCTCATGCGAAGGGATTATGGTTAAATCTTTAGATGTTGATGCTGGATATTCTCCATCAAAGCGTACAGACACATGGCTAAAGGTCAAGCGAGACTACATGGAGGGATTGAACGACTCTCTTGATTTAGTCCCAATTGGAGCTTGGCATGGAAACGGGAGAAAGGCAGGCTGGTACAGTCCATTTCTCATGGCATGTTATAACCCTGAGACTGAAGATTTCCAAAGCGTCTGCCGTGTCATGTCTGGGTTTTCCGACTCATTTTACATTGAGATGAAAGGTTTCTTTTCGGGAGCCAAGATCTTGTCCAGAAAACCACCTTATTATAAAACCTCTGAGATGCCTGATATGTGGTTTTCTCCGGAACTTGTGTGGGAAATAAGAGGTGCAGACTTTACAGTGTCTCCTGTTCACCAGGCTGCTGTAGGTTTAGTGCATCCATCACGCGGCATTTCAATCAGATTCCCAAGGTACATTCGCACTGTGGCAGATAGAAAACCAGATGAATGTAGCACAGCTGAAGATATAGCTGCAATGTTTCGTTCTCAGACAAGGAAGATGGACGTGGCAGCTGAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000012 GO:0000726 GO:0002237 GO:0003006 GO:0003674 GO:0003824 GO:0003909 GO:0003910 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006266 GO:0006271 GO:0006273 GO:0006281 GO:0006302 GO:0006303 GO:0006304 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009314 GO:0009409 GO:0009411 GO:0009416 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009791 GO:0009845 GO:0009987 GO:0010033 GO:0010154 GO:0010225 GO:0010243 GO:0015074 GO:0016070 GO:0016874 GO:0016886 GO:0022414 GO:0022616 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0033554 GO:0034641 GO:0034645 GO:0035510 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044728 GO:0046483 GO:0046677 GO:0048316 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051103 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0065007 GO:0070013 GO:0070988 GO:0071704 GO:0080111 GO:0090304 GO:0090351 GO:0140097 GO:1901360 GO:1901576 GO:1901652 GO:1901698 GO:1901700 GO:1904975 GO:2000683 GO:2000685
KEGG Pathways
Metabolic & Signaling
ko03030 ko03410 ko03420 ko03430 map03030 map03410 map03420 map03430
Pfam Domains
Protein Families
DNA_ligase_A_C DNA_ligase_A_M DNA_ligase_A_N DRMBL

Protein Analysis

1415

Amino Acids

157.64

Weight (kDa)

6.24

Isoelectric Point (pI)

42.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DRMBL PF07522 271 - 373 1.1e-20 DNA repair metallo-beta-lactamase
DNA_ligase_A_N PF04675 778 - 954 5.2e-42 DNA ligase N terminus
DNA_ligase_A_M PF01068 1030 - 1251 2e-48 ATP dependent DNA ligase domain
DNA_ligase_A_C PF04679 1277 - 1382 3.9e-16 ATP dependent DNA ligase C terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015031)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66730
fragaria_vesca FvH4_1g29000 FvH4_1g29000 FvH4_1g29000
malus_domestica MD00G1054100.v1.1
prunus_persica Prupe.3G150300_v2.0.a1
pyrus_communis pycom17g25560
rosa_chinensis RchiOBHm_Chr3g0493691 RchiOBHm_Chr3g0493951
rosa_laevigata RLG00000022694
rosa_roxburghii Rroxscaffold_6G00391370
rosa_rugosa Rorug03G0263400 Rorug03G0263500 Rorug03G0263600
rosa_samantha Rh3AG312500 Rh3AG313200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 3864, 3990
Acc16I TGCGCA 1 cut(s) 735
Acc36I ACCTGC 2 cut(s) 2221, 2860
AccI GTMKAC 2 cut(s) 174, 3441
AccII CGCG 3 cut(s) 1554, 3219, 4110
AccIII TCCGGA 2 cut(s) 445, 4024
AclWI GGATC 2 cut(s) 1021, 2562
AcoI YGGCCR 2 cut(s) 881, 924
AcsI RAATTY 9 cut(s) 441, 533, 584, 1562, 1804, 1930, 2815, 3189, 3652
AcuI CTGAAG 6 cut(s) 1967, 2442, 2918, 3149, 3897, 4203
AcvI CACGTG 1 cut(s) 3389
AcyI GRCGYC 1 cut(s) 273
AfaI GTAC 9 cut(s) 846, 1038, 1681, 2588, 2709, 2966, 3730, 3839, 4136
AfeI AGCGCT 1 cut(s) 2064
AfiI CCNNNNNNNGG 9 cut(s) 261, 262, 1118, 1288, 1289, 1290, 1649, 2577, 4109
AflIII ACRYGT 3 cut(s) 847, 2412, 3155
AjiI CACGTC 2 cut(s) 2449, 4228
AjnI CCWGG 3 cut(s) 1750, 3070, 4077
AjuI GAANNNNNNNTTGG 6 cut(s) 28, 60, 149, 181, 2203, 2235
AleI CACNNNNGTG 1 cut(s) 314
AloI GAACNNNNNNTCC 2 cut(s) 2715, 2747
Alw21I GWGCWC 2 cut(s) 321, 2474
Alw44I GTGCAC 1 cut(s) 2470
AlwI GGATC 2 cut(s) 1021, 2562
AlwNI CAGNNNCTG 4 cut(s) 1655, 1886, 2951, 3493
Aor13HI TCCGGA 2 cut(s) 445, 4024
Aor51HI AGCGCT 1 cut(s) 2064
AoxI GGCC 6 cut(s) 3, 247, 881, 924, 1279, 1826
ApaI GGGCCC 1 cut(s) 1283
ApaLI GTGCAC 1 cut(s) 2470
ApeKI GCWGC 8 cut(s) 2021, 2452, 2486, 2948, 3639, 4082, 4193, 4232
ApoI RAATTY 9 cut(s) 441, 533, 584, 1562, 1804, 1930, 2815, 3189, 3652
Asp700I GAANNNNTTC 2 cut(s) 1211, 3944
AspLEI GCGC 4 cut(s) 736, 2065, 2766, 3219
AspS9I GGNCC 4 cut(s) 1279, 1280, 1826, 2097
AsuC2I CCSGG 2 cut(s) 417, 3555
AsuII TTCGAA 2 cut(s) 2734, 3564
AvaII GGWCC 1 cut(s) 2097
BaeGI GKGCMC 3 cut(s) 1283, 2474, 3187
BaeI ACNNNNGTAYC 2 cut(s) 1961, 1994
BalI TGGCCA 1 cut(s) 883
BanII GRGCYC 2 cut(s) 1283, 1296
BbrPI CACGTG 1 cut(s) 3389
BbsI GAAGAC 1 cut(s) 296
Bbv12I GWGCWC 2 cut(s) 321, 2474
BbvI GCAGC 7 cut(s) 2008, 2439, 2473, 2935, 3626, 4069, 4180
BccI CCATC 9 cut(s) 111, 371, 1038, 1988, 3167, 3200, 3727, 4111, 4216
BceAI ACGGC 4 cut(s) 155, 259, 350, 3882
BciT130I CCWGG 3 cut(s) 1752, 3072, 4079
BciVI GTATCC 1 cut(s) 929
BcnI CCSGG 2 cut(s) 417, 3555
BfaI CTAG 6 cut(s) 627, 1493, 1587, 2009, 2606, 3317
BfmI CTRYAG 5 cut(s) 612, 1113, 2295, 2952, 4086
BfoI RGCGCY 1 cut(s) 2066
BfuAI ACCTGC 2 cut(s) 2221, 2860
BfuI GTATCC 1 cut(s) 929
BglI GCCNNNNNGGC 1 cut(s) 192
BglII AGATCT 2 cut(s) 3262, 3963
BmcAI AGTACT 1 cut(s) 1681
Bme1390I CCNGG 5 cut(s) 417, 1752, 3072, 3555, 4079
Bme18I GGWCC 1 cut(s) 2097
BmgBI CACGTC 2 cut(s) 2449, 4228
BmgT120I GGNCC 4 cut(s) 1279, 1280, 1826, 2097
BmiI GGNNCC 6 cut(s) 544, 1281, 1282, 2208, 3069, 3958
BmrFI CCNGG 5 cut(s) 417, 1752, 3072, 3555, 4079
BpiI GAAGAC 1 cut(s) 296
BpmI CTGGAG 2 cut(s) 2054, 2332
Bpu14I TTCGAA 2 cut(s) 2734, 3564
BpuEI CTTGAG 4 cut(s) 305, 2387, 2604, 2847
BpuMI CCSGG 2 cut(s) 417, 3555
Bsa29I ATCGAT 1 cut(s) 375
BsaAI YACGTR 2 cut(s) 570, 3389
BsaHI GRCGYC 1 cut(s) 273
BsaI GGTCTC 2 cut(s) 331, 3216
BsaJI CCNNGG 5 cut(s) 1721, 1751, 3057, 3573, 4130
BsaWI WCCGGW 3 cut(s) 445, 2099, 4024
BsaXI ACNNNNNCTCC 6 cut(s) 191, 203, 221, 233, 4051, 4081
Bsc4I CCNNNNNNNGG 9 cut(s) 261, 262, 1118, 1288, 1289, 1290, 1649, 2577, 4109
Bse118I RCCGGY 2 cut(s) 191, 2628
Bse1I ACTGG 7 cut(s) 487, 970, 1155, 1842, 2243, 2547, 3612
Bse3DI GCAATG 4 cut(s) 1901, 2838, 3069, 4203
BseAI TCCGGA 2 cut(s) 445, 4024
BseBI CCWGG 3 cut(s) 1752, 3072, 4079
BseCI ATCGAT 1 cut(s) 375
BseDI CCNNGG 5 cut(s) 1721, 1751, 3057, 3573, 4130
BseGI GGATG 9 cut(s) 103, 150, 769, 832, 1132, 1969, 2377, 2683, 4099
BseLI CCNNNNNNNGG 9 cut(s) 261, 262, 1118, 1288, 1289, 1290, 1649, 2577, 4109
BseMI GCAATG 4 cut(s) 1901, 2838, 3069, 4203
BseMII CTCAG 5 cut(s) 303, 2204, 3861, 3990, 4223
BseNI ACTGG 7 cut(s) 487, 970, 1155, 1842, 2243, 2547, 3612
BseRI GAGGAG 5 cut(s) 4, 321, 1316, 2051, 2872
BseSI GKGCMC 3 cut(s) 1283, 2474, 3187
BseXI GCAGC 7 cut(s) 2008, 2439, 2473, 2935, 3626, 4069, 4180
BseYI CCCAGC 4 cut(s) 355, 1178, 2596, 3493
BsgI GTGCAG 3 cut(s) 2249, 2673, 4071
Bsh1236I CGCG 3 cut(s) 1554, 3219, 4110
BshFI GGCC 6 cut(s) 5, 249, 883, 926, 1281, 1828
BshVI ATCGAT 1 cut(s) 375
BsiHKAI GWGCWC 2 cut(s) 321, 2474
BsiSI CCGG 7 cut(s) 192, 416, 446, 2100, 2629, 3554, 4025
BslFI GGGAC 5 cut(s) 1532, 1591, 2572, 3251, 3779
BslI CCNNNNNNNGG 9 cut(s) 261, 262, 1118, 1288, 1289, 1290, 1649, 2577, 4109
BsmBI CGTCTC 1 cut(s) 211
BsmFI GGGAC 5 cut(s) 1532, 1591, 2572, 3251, 3779
BsmI GAATGC 2 cut(s) 179, 2332
BsnI GGCC 6 cut(s) 5, 249, 883, 926, 1281, 1828
Bso31I GGTCTC 2 cut(s) 331, 3216
Bsp119I TTCGAA 2 cut(s) 2734, 3564
Bsp120I GGGCCC 1 cut(s) 1279
Bsp1286I GDGCHC 5 cut(s) 321, 1283, 1296, 2474, 3187
Bsp13I TCCGGA 2 cut(s) 445, 4024
Bsp1407I TGTACA 3 cut(s) 844, 2586, 2707
BspANI GGCC 6 cut(s) 5, 249, 883, 926, 1281, 1828
BspCNI CTCAG 5 cut(s) 304, 2203, 3862, 3991, 4222
BspDI ATCGAT 1 cut(s) 375
BspEI TCCGGA 2 cut(s) 445, 4024
BspFNI CGCG 3 cut(s) 1554, 3219, 4110
BspHI TCATGA 1 cut(s) 1567
BspLI GGNNCC 6 cut(s) 544, 1281, 1282, 2208, 3069, 3958
BspMI ACCTGC 2 cut(s) 2221, 2860
BspPI GGATC 2 cut(s) 1021, 2562
BspQI GCTCTTC 2 cut(s) 1214, 2176
BspT104I TTCGAA 2 cut(s) 2734, 3564
BspTNI GGTCTC 2 cut(s) 331, 3216
BsrDI GCAATG 4 cut(s) 1901, 2838, 3069, 4203
BsrFI RCCGGY 2 cut(s) 191, 2628
BsrGI TGTACA 3 cut(s) 844, 2586, 2707
BsrI ACTGG 7 cut(s) 487, 970, 1155, 1842, 2243, 2547, 3612
BssAI RCCGGY 2 cut(s) 191, 2628
BssECI CCNNGG 5 cut(s) 1721, 1751, 3057, 3573, 4130
BssNI GRCGYC 1 cut(s) 273
BssT1I CCWWGG 4 cut(s) 1721, 3057, 3573, 4130
Bst2UI CCWGG 3 cut(s) 1752, 3072, 4079
Bst6I CTCTTC 5 cut(s) 22, 62, 1214, 2176, 2902
BstACI GRCGYC 1 cut(s) 273
BstAPI GCANNNNNTGC 1 cut(s) 3599
BstAUI TGTACA 3 cut(s) 844, 2586, 2707
BstBAI YACGTR 2 cut(s) 570, 3389
BstBI TTCGAA 2 cut(s) 2734, 3564
BstDEI CTNAG 7 cut(s) 312, 942, 1070, 2190, 3870, 3999, 4209
BstEII GGTNACC 1 cut(s) 1474
BstF5I GGATG 9 cut(s) 103, 150, 769, 832, 1132, 1969, 2377, 2683, 4099
BstFNI CGCG 3 cut(s) 1554, 3219, 4110
BstH2I RGCGCY 1 cut(s) 2066
BstHHI GCGC 4 cut(s) 736, 2065, 2766, 3219
BstMWI GCNNNNNNNGC 7 cut(s) 71, 74, 176, 192, 3514, 3599, 3626
BstNI CCWGG 3 cut(s) 1752, 3072, 4079
BstNSI RCATGY 8 cut(s) 761, 851, 2306, 2416, 2556, 2628, 3159, 3861
BstPI GGTNACC 1 cut(s) 1474
BstSCI CCNGG 5 cut(s) 415, 1750, 3070, 3553, 4077
BstSFI CTRYAG 5 cut(s) 612, 1113, 2295, 2952, 4086
BstSLI GKGCMC 3 cut(s) 1283, 2474, 3187
BstSNI TACGTA 1 cut(s) 570
BstUI CGCG 3 cut(s) 1554, 3219, 4110
BstV1I GCAGC 7 cut(s) 2008, 2439, 2473, 2935, 3626, 4069, 4180
BstV2I GAAGAC 1 cut(s) 296
BstX2I RGATCY 3 cut(s) 1026, 3262, 3963
BstXI CCANNNNNNTGG 1 cut(s) 2032
BstYI RGATCY 3 cut(s) 1026, 3262, 3963
Bsu15I ATCGAT 1 cut(s) 375
BsuI GTATCC 1 cut(s) 929
BsuRI GGCC 6 cut(s) 5, 249, 883, 926, 1281, 1828
BsuTUI ATCGAT 1 cut(s) 375
BtrI CACGTC 2 cut(s) 2449, 4228
BtsCI GGATG 9 cut(s) 103, 150, 769, 832, 1132, 1969, 2377, 2683, 4099
BtsI GCAGTG 1 cut(s) 2149
BveI ACCTGC 2 cut(s) 2221, 2860
CaiI CAGNNNCTG 4 cut(s) 1655, 1886, 2951, 3493
CciI TCATGA 1 cut(s) 1567
CfoI GCGC 4 cut(s) 736, 2065, 2766, 3219
Cfr10I RCCGGY 2 cut(s) 191, 2628
Cfr13I GGNCC 4 cut(s) 1279, 1280, 1826, 2097
ClaI ATCGAT 1 cut(s) 375
CseI GACGC 5 cut(s) 185, 262, 410, 2927, 3880
Csp6I GTAC 9 cut(s) 845, 1037, 1680, 2587, 2708, 2965, 3729, 3838, 4135
CviQI GTAC 9 cut(s) 845, 1037, 1680, 2587, 2708, 2965, 3729, 3838, 4135
DdeI CTNAG 7 cut(s) 312, 942, 1070, 2190, 3870, 3999, 4209
DraI TTTAAA 1 cut(s) 439
EaeI YGGCCR 2 cut(s) 881, 924
Eam1104I CTCTTC 5 cut(s) 22, 62, 1214, 2176, 2902
EarI CTCTTC 5 cut(s) 22, 62, 1214, 2176, 2902
EciI GGCGGA 1 cut(s) 173
Eco105I TACGTA 1 cut(s) 570
Eco130I CCWWGG 4 cut(s) 1721, 3057, 3573, 4130
Eco24I GRGCYC 2 cut(s) 1283, 1296
Eco31I GGTCTC 2 cut(s) 331, 3216
Eco32I GATATC 2 cut(s) 2053, 3466
Eco47I GGWCC 1 cut(s) 2097
Eco47III AGCGCT 1 cut(s) 2064
Eco57I CTGAAG 6 cut(s) 1967, 2442, 2918, 3149, 3897, 4203
Eco72I CACGTG 1 cut(s) 3389
Eco91I GGTNACC 1 cut(s) 1474
EcoO109I RGGNCCY 2 cut(s) 1279, 1280
EcoO65I GGTNACC 1 cut(s) 1474
EcoRII CCWGG 3 cut(s) 1750, 3070, 4077
EcoRV GATATC 2 cut(s) 2053, 3466
EcoT14I CCWWGG 4 cut(s) 1721, 3057, 3573, 4130
EcoT22I ATGCAT 1 cut(s) 3286
EcoT38I GRGCYC 2 cut(s) 1283, 1296
ErhI CCWWGG 4 cut(s) 1721, 3057, 3573, 4130
Esp3I CGTCTC 1 cut(s) 211
FalI AAGNNNNNCTT 4 cut(s) 1199, 1231, 2916, 2948
FaqI GGGAC 5 cut(s) 1532, 1591, 2572, 3251, 3779
FauI CCCGC 1 cut(s) 1817
FauNDI CATATG 1 cut(s) 1321
FblI GTMKAC 2 cut(s) 174, 3441
FokI GGATG 9 cut(s) 90, 137, 776, 839, 1139, 1976, 2384, 2690, 4086
FriOI GRGCYC 2 cut(s) 1283, 1296
FspBI CTAG 6 cut(s) 627, 1493, 1587, 2009, 2606, 3317
FspI TGCGCA 1 cut(s) 735
GlaI GCGC 4 cut(s) 735, 2064, 2765, 3218
GsaI CCCAGC 4 cut(s) 359, 1182, 2600, 3497
GsuI CTGGAG 2 cut(s) 2054, 2332
HaeII RGCGCY 1 cut(s) 2066
HaeIII GGCC 6 cut(s) 5, 249, 883, 926, 1281, 1828
HapII CCGG 7 cut(s) 192, 416, 446, 2100, 2629, 3554, 4025
HgaI GACGC 5 cut(s) 185, 262, 410, 2927, 3880
HhaI GCGC 4 cut(s) 736, 2065, 2766, 3219
Hin1I GRCGYC 1 cut(s) 273
Hin6I GCGC 4 cut(s) 734, 2063, 2764, 3217
HinP1I GCGC 4 cut(s) 734, 2063, 2764, 3217
HincII GTYRAC 5 cut(s) 175, 1774, 3054, 3246, 3442
HindII GTYRAC 5 cut(s) 175, 1774, 3054, 3246, 3442
HindIII AAGCTT 3 cut(s) 1136, 2930, 3147
HpaI GTTAAC 1 cut(s) 1774
HpaII CCGG 7 cut(s) 192, 416, 446, 2100, 2629, 3554, 4025
Hpy99I CGWCG 3 cut(s) 176, 179, 278
HpyCH4IV ACGT 6 cut(s) 569, 988, 2448, 3388, 3623, 4227
HpyF10VI GCNNNNNNNGC 7 cut(s) 71, 74, 176, 192, 3514, 3599, 3626
HpyF3I CTNAG 7 cut(s) 312, 942, 1070, 2190, 3870, 3999, 4209
HpySE526I ACGT 6 cut(s) 569, 988, 2448, 3388, 3623, 4227
Hsp92I GRCGYC 1 cut(s) 273
HspAI GCGC 4 cut(s) 734, 2063, 2764, 3217
Kpn2I TCCGGA 2 cut(s) 445, 4024
KroI GCCGGC 2 cut(s) 191, 2628
KroNI GCCGGC 2 cut(s) 193, 2630
KspAI GTTAAC 1 cut(s) 1774
LguI GCTCTTC 2 cut(s) 1214, 2176
LmnI GCTCC 5 cut(s) 2335, 2506, 2828, 3803, 3956
Lsp1109I GCAGC 7 cut(s) 2008, 2439, 2473, 2935, 3626, 4069, 4180
MaeI CTAG 6 cut(s) 627, 1493, 1587, 2009, 2606, 3317
MaeII ACGT 6 cut(s) 569, 988, 2448, 3388, 3623, 4227
MaeIII GTNAC 7 cut(s) 22, 1474, 1625, 2530, 2643, 3353, 3384
MfeI CAATTG 2 cut(s) 259, 3798
MflI RGATCY 3 cut(s) 1026, 3262, 3963
MhlI GDGCHC 5 cut(s) 321, 1283, 1296, 2474, 3187
MlsI TGGCCA 1 cut(s) 883
MluNI TGGCCA 1 cut(s) 883
MlyI GAGTC 6 cut(s) 617, 1277, 1622, 3251, 3773, 3914
MmeI TCCRAC 5 cut(s) 950, 1296, 2199, 3019, 3942
Mox20I TGGCCA 1 cut(s) 883
Mph1103I ATGCAT 1 cut(s) 3286
MreI CGCCGGCG 1 cut(s) 191
MroI TCCGGA 2 cut(s) 445, 4024
MroNI GCCGGC 2 cut(s) 191, 2628
MroXI GAANNNNTTC 2 cut(s) 1211, 3944
MscI TGGCCA 1 cut(s) 883
MslI CAYNNNNRTG 4 cut(s) 314, 762, 2499, 3935
Msp20I TGGCCA 1 cut(s) 883
MspA1I CMGCKG 5 cut(s) 355, 2948, 3639, 4181, 4235
MspI CCGG 7 cut(s) 192, 416, 446, 2100, 2629, 3554, 4025
MspR9I CCNGG 5 cut(s) 417, 1752, 3072, 3555, 4079
MunI CAATTG 2 cut(s) 259, 3798
Mva1269I GAATGC 2 cut(s) 179, 2332
MvaI CCWGG 3 cut(s) 1752, 3072, 4079
MvnI CGCG 3 cut(s) 1554, 3219, 4110
MwoI GCNNNNNNNGC 7 cut(s) 71, 74, 176, 192, 3514, 3599, 3626
NaeI GCCGGC 2 cut(s) 193, 2630
NciI CCSGG 2 cut(s) 417, 3555
NdeI CATATG 1 cut(s) 1321
NgoMIV GCCGGC 2 cut(s) 191, 2628
NlaIV GGNNCC 6 cut(s) 544, 1281, 1282, 2208, 3069, 3958
NmuCI GTSAC 6 cut(s) 22, 1474, 1625, 2530, 3353, 3384
NsbI TGCGCA 1 cut(s) 735
NsiI ATGCAT 1 cut(s) 3286
NspI RCATGY 8 cut(s) 761, 851, 2306, 2416, 2556, 2628, 3159, 3861
NspV TTCGAA 2 cut(s) 2734, 3564
OliI CACNNNNGTG 1 cut(s) 314
PaeI GCATGC 1 cut(s) 2628
PagI TCATGA 1 cut(s) 1567
PciI ACATGT 3 cut(s) 847, 2412, 3155
PciSI GCTCTTC 2 cut(s) 1214, 2176
PcsI WCGNNNNNNNCGW 3 cut(s) 1007, 1016, 3447
PctI GAATGC 2 cut(s) 179, 2332
PdiI GCCGGC 2 cut(s) 193, 2630
PdmI GAANNNNTTC 2 cut(s) 1211, 3944
PfeI GAWTC 8 cut(s) 44, 1016, 1598, 1817, 2003, 2219, 2755, 4125
PfoI TCCNGGA 1 cut(s) 3070
PleI GAGTC 6 cut(s) 616, 1276, 1622, 3250, 3773, 3914
PmaCI CACGTG 1 cut(s) 3389
PmlI CACGTG 1 cut(s) 3389
PpsI GAGTC 6 cut(s) 616, 1276, 1622, 3250, 3773, 3914
Ppu21I YACGTR 2 cut(s) 570, 3389
PscI ACATGT 3 cut(s) 847, 2412, 3155
PsiI TTATAA 2 cut(s) 3864, 3990
Psp6I CCWGG 3 cut(s) 1750, 3070, 4077
PspCI CACGTG 1 cut(s) 3389
PspEI GGTNACC 1 cut(s) 1474
PspFI CCCAGC 4 cut(s) 355, 1178, 2596, 3493
PspGI CCWGG 3 cut(s) 1750, 3070, 4077
PspN4I GGNNCC 6 cut(s) 544, 1281, 1282, 2208, 3069, 3958
PspOMI GGGCCC 1 cut(s) 1279
PspPI GGNCC 4 cut(s) 1279, 1280, 1826, 2097
PstNI CAGNNNCTG 4 cut(s) 1655, 1886, 2951, 3493
PsuI RGATCY 3 cut(s) 1026, 3262, 3963
PvuII CAGCTG 4 cut(s) 2948, 3639, 4181, 4235
RsaI GTAC 9 cut(s) 846, 1038, 1681, 2588, 2709, 2966, 3730, 3839, 4136
RsaNI GTAC 9 cut(s) 845, 1037, 1680, 2587, 2708, 2965, 3729, 3838, 4135
RseI CAYNNNNRTG 4 cut(s) 314, 762, 2499, 3935
SalI GTCGAC 2 cut(s) 173, 3440
SapI GCTCTTC 2 cut(s) 1214, 2176
Sau96I GGNCC 4 cut(s) 1279, 1280, 1826, 2097
ScaI AGTACT 1 cut(s) 1681
SchI GAGTC 6 cut(s) 617, 1277, 1622, 3251, 3773, 3914
ScrFI CCNGG 5 cut(s) 417, 1752, 3072, 3555, 4079
SduI GDGCHC 5 cut(s) 321, 1283, 1296, 2474, 3187
SfcI CTRYAG 5 cut(s) 612, 1113, 2295, 2952, 4086
SfuI TTCGAA 2 cut(s) 2734, 3564
SgrAI CRCCGGYG 1 cut(s) 191
SgrDI CGTCGACG 1 cut(s) 173
SinI GGWCC 1 cut(s) 2097
SmiMI CAYNNNNRTG 4 cut(s) 314, 762, 2499, 3935
SmlI CTYRAG 4 cut(s) 320, 2366, 2619, 2862
SmoI CTYRAG 4 cut(s) 320, 2366, 2619, 2862
SnaBI TACGTA 1 cut(s) 570
SphI GCATGC 1 cut(s) 2628
SspMI CTAG 6 cut(s) 627, 1493, 1587, 2009, 2606, 3317
StyD4I CCNGG 5 cut(s) 415, 1750, 3070, 3553, 4077
StyI CCWWGG 4 cut(s) 1721, 3057, 3573, 4130
TaiI ACGT 6 cut(s) 572, 991, 2451, 3391, 3626, 4230
TaqI TCGA 9 cut(s) 174, 375, 1010, 1396, 2734, 2958, 3339, 3441, 3564
TatI WGTACW 4 cut(s) 844, 1679, 2586, 2707
TauI GCSGC 6 cut(s) 68, 77, 249, 355, 3511, 4113
TfiI GAWTC 8 cut(s) 44, 1016, 1598, 1817, 2003, 2219, 2755, 4125
TseFI GTSAC 6 cut(s) 22, 1474, 1625, 2530, 3353, 3384
TseI GCWGC 8 cut(s) 2021, 2452, 2486, 2948, 3639, 4082, 4193, 4232
Tsp45I GTSAC 6 cut(s) 22, 1474, 1625, 2530, 3353, 3384
TspGWI ACGGA 3 cut(s) 10, 193, 471
VneI GTGCAC 1 cut(s) 2470
VpaK11BI GGWCC 1 cut(s) 2097
XapI RAATTY 9 cut(s) 441, 533, 584, 1562, 1804, 1930, 2815, 3189, 3652
XbaI TCTAGA 1 cut(s) 1586
XceI RCATGY 8 cut(s) 761, 851, 2306, 2416, 2556, 2628, 3159, 3861
XcmI CCANNNNNNNNNTGG 1 cut(s) 3804
XmiI GTMKAC 2 cut(s) 174, 3441
XmnI GAANNNNTTC 2 cut(s) 1211, 3944
XspI CTAG 6 cut(s) 627, 1493, 1587, 2009, 2606, 3317
ZrmI AGTACT 1 cut(s) 1681
Zsp2I ATGCAT 1 cut(s) 3286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.