Rh3AG312500

DNA ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0015031
Physical Location & Seq
Reverse (-)
39333134 .. 39344266
11133 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG312500.1

Sequence Viewer

Length: 4212 bp
ATGATGTTCTTGATGACGGCTCTAGATTCTACGGACCTCTTCCTGACTGCCCTAACCGCCTTTTCCCACCCACCCAGACCCATACCCCGTCCCCTTGAACTCTCTCTCCCTCCAATTCCCTCCACATTCCCCCACTCCAAACTCATCCCCAGAACCCGTTTCGCCCTCGACGCATTCCGCTATGCCGGCGATTATTCCGTCTCTTACTTCCTCTCTCACTTCCACTCCGATCACTACGGCGGTCTCTCCCCCAATTGGTCCCAAGGCCTCGTCTTTTGCTCCCCCACCACCGCTCGTCTTCTCACAGACGTCCTCAACGTCTCTTCGCTTTTCGTGGTTCCTCTGCCGCTCGGACAGCCCCTTCTCATCGATGGATGCGAGGTCGTGCTTATTGACGCCAATCATTGCCCGGGCGCCGTTCAGTTCTTGTTCAAAGTTCCCGCCTCCAATGGAGACTTCCAGAGGTATGTTCACACTGGTGATTTTCGGTTTTCTGCTCCGATGAAGCTAGACCCTTTTCTCCGTAACTTTGTGGGTTCAGACGCCGTTTTCTTGGATACAACTTATTGCAATCCCAAATTCGTGTTTCCTTCACAAGAAGAATCTGTTGATTATATTTCTAGCTTGATAGAAAGAGTCGGAGGTGAACACGAAGTTTCTATGAAAAGTGTTCTGTTCCTTGTTGCTACATATGTGATTGGGAAAGAGAAGATTTTGATAGAAATTGCTCGTAGATGTAAGCGTAAAGTGTATGTGGATGCTCGAAAAATGGCGGTGCTGCGTGTTTTGGGTTACGGAGACAGTGGGGTGTTTACAGAGGATGAATGTGAGAGTGATGTGCATGTTGTTGGGTGGAATGTGTTGGGTGAGACTTGGCCATATTTCAGGCCAAATTTTGTGAAAATGGAGGAGATTATGGTTGAGAGGGGGTATTCCAGGGTTGTAGGGTTTGTCCCTACTGGCTGGACTTATGAAGTCAAGCGTAACAAGTTCTCGGTGAGGTCAAAGGATGCTTTTGAGATCCATCTTGTACCCTACAGTGAGCATTCAAACTATGATGAACTTAGAGAATACGTCAGGTTTTTGAAACCAAAGCGTGTGATTCCTACAGTGGGCTCCGATGTGGAAAAGATAGACAGTAAACAGGCCAGTAAAATGCAAAAGCATTTTGCTGGGTTAATTGATGAAATGGCCAACAAAAAAGAGTTCCTGCAGGGTTTTCATCGTGGGACTACAGAAGGAGGTGGGAAGGTTGACAGTGATGCCAAAGATTACACCATGGAGGGGCAGGATCAAGAGAAAAAGGCAACACCTTCAGATATTGATACTATGGGAGATACTAATGTAGGCTTTGGTAAGGAATTGTTGTCTCCTCTCCAAGAACCTGATTCACAAACTCCAATATTGTTAACTGATGAAAAAGCAGAGGAAATTATTCAACAACTTCGTGATTGTTTGCCGTCATGGGTCACTCGCCAGCAGATGCTAGATCTGATTGGGAGCTCAGGAGGGGACATTGTTGAAGCAGTCACTAAATTTTACGATCGTGAAACAGAATTTCATGATCAAGGAATTGCCTCTACAAGTGCCGTATCTGTATCCGAGACCAGCTCACTCTGTGACACAGCATCACCGACAAAAGCAGGTTCTGTTCATGCAAATATTGATGTTTCTTCAAGCCAAGCTCATATTTCACCCAACCCAAGAAATATTGTTAAAAGTGGCATTTCCCCTGGAAAAAGAGGGAGGAAGATCAGTAATAAAGTTAACAAGAAAGTAAAGTTACAGTCAAAATTGAATTTGTGTGGGCCAAAACAATCGACAATAACAAGGTTTTTTAGTAAAGTTTTGCCTGACGTTACAGAAAGTGCAGAGATTGGATCAGTAGGTGAACAAAACCCTAAAGATAAAAAATTACCAGATCATGTCACCCAATCATACAAGGATGCAATAGATCAATTTCTTCAGATAATAGATGGCAATGAATCATTAAAAAGCTATGCTGGTACCATTCTTCAAAAGGCAAATGGGGATATTAGTATGGCTGTGGATATACATTACCGTAATAATGAAGGCAGGTCTGATGAGAATGAAGTGGAGCTGGTGGTTGATGCAAAATCAGTTCAGTCCAAATGTGGTATAGATAACTCTTCTTCTGTCCAAAAAAAAATTGAATTAGGGAAAATAGGAGTGGTAGCTGATTTGTCTGTACGGGGGTCATTACCAGATAACGTCGCTGCAACTTCTGTGTCACTACCACCAGAAAAATACAATCCTGTAGATCATGCATGCTGGAGCAATGGACAACATGCTCCATATTTACATCTTGCACGAACCTTTGACTTACTAGAGGATGAAAAAGGCAAGATAAAGGCTACTTCTATGCTCTGCAATATGTTCAGAAGTTTGCTTGCCCTGTCTCCTGATGATGTGCTGCCTTCAGTTTATTTGTGCACCAATAAGATAGCTGCAGACCACGAAAATGTGGAACTGAACATTGGTGGAAGTTTGGTCACAGCAGCACTGGAGGACGCATGTGGGACAAGCAGATCCAAAATCAGGGATATGTACAATGAATTGGGAGATCTTGGTGATGTTGCTCAAGCATGCCGGCAGACACAGACTTTGCTTGCCCCTCCTCCACCACTTCTAATAAAAGATGTATTTTCTGCACTCCGGAAGATAAGTGTACAAACAGGTACTGGAAGCAGTGCTCGAAAGAGAAGCCTGATTGTGAATCTCTTGCGTTCTTGCAGAGAGAAAGAGATGAAGTTCCTTGTTAGAACTTTGGTTCGAAACTTACGTATCGGAGCAATGATGAAAACTGTCCTACCTGCTCTGGCTCAAGCTGTTGTTCTGAATTCCTTCCATAGCTGTAATCACGAAGGAACTATAGAAAGTTTGAAGGACAAGCTTCAGCGCCATTCAGCAGCTGTAGTAGAAGCATACAATGTACTGCCCAATTTGGATTTGGTCATTCCTTCTCTCATGAAAAGAGGCATTGGATTCTCCTCATCAACTTTGTCAATGGTTCCAGGAATACCTATCAAGCCAATGCTTGCAAGAATCACAAATGGTGTTCAGCAAGCACTGAAGCTATTTGAAAATAAAGCTTTTACATGTGAATACAAATATGATGGCCAGCGTGCACAAATTCATAAACTAGTTGATGGGTCCATGCATGTTTTTACAAGAAATGGGGATGAGTCAACTTCGAGATTTCCAGATCTGATTAATATAATCAATCAATCTTGTAAACCCGATGCAGTGACTTTCATACTGGATGCAGAGGTTGTTGGAGTTGATAGGAAGAATGGTTGCAAGCTAATGTCCTTCCAAGAATTATCTTCACGTGGGAGAGGGAGCAGAGATGCATCAATAACGTTGGATAGCATAAAGGTCAACATTTGCGTCTTTGTCTTCGACATCATGTTTGCCAATGGACAACAGTTGTTAAGCTTTCCTCTGCGGAGAAGGCGAAAGTATTTGAAAGAGTTGTTCTACGATGAGAAGTTGGGTTACTTTGAATATGCCAAGGAAATGACAGTTGAGGCAGAGGATGCTTGTTTAACCAGTGAAGCGACATTAACCAAAATAAACTCTTTCCTTGAAAATGCATTTGTTTCCTCTTGTGAAGGGATTATAGTTAAATCTTTAGATGTTGATGCTGGATATTCTCCATCAAAGCGTACAGATACTTGGTTAAAGGTCAAGCGAGACTACATGGAGGGATTGGGCGATACTCTTGATTTAGTCCCAATTGGAGCTTGGCATGGAAACGGGAGAAAGGCAGGCTGGTACAGTCCATTTCTCATGGCATGTTACAATCCTGAAACTGAAGAATTTCAAAGCGTCTGTCGTGTTATGTCTGGGTTTTCAGATTTATTTTATACAGAGATGAAAAGTTTTTATTCTGGGGACAAGATCTTGGCCAAAAAACCTCCTTATTATAGAACAGCTGAGGTGCCTGATTTGTGGTTTTCTCCTGAGCTTGTATGGGAAATAAGAGGTGCAGACTTTACAGTGTCTCCAGTTCACCAGGCTGCTTTAGGTCTAGTTCATCCATCACGTGGCATTTCCATTCGATTCCCAAGATACATTCGCACTGTGACTGATAGAAAACCAGATGAATGTAGCACAGCTTCTGATTTAGCTGAAATGTTTCATTCTCAGACGAGGAAGATGGATGTGGCTTCTGAAGATGGCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000012 GO:0000726 GO:0002237 GO:0003006 GO:0003674 GO:0003824 GO:0003909 GO:0003910 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006266 GO:0006271 GO:0006273 GO:0006281 GO:0006302 GO:0006303 GO:0006304 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009314 GO:0009409 GO:0009411 GO:0009416 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009791 GO:0009845 GO:0009987 GO:0010033 GO:0010154 GO:0010225 GO:0010243 GO:0015074 GO:0016070 GO:0016874 GO:0016886 GO:0022414 GO:0022616 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0033554 GO:0034641 GO:0034645 GO:0035510 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044728 GO:0046483 GO:0046677 GO:0048316 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051103 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0065007 GO:0070013 GO:0070988 GO:0071704 GO:0080111 GO:0090304 GO:0090351 GO:0140097 GO:1901360 GO:1901576 GO:1901652 GO:1901698 GO:1901700 GO:1904975 GO:2000683 GO:2000685
KEGG Pathways
Metabolic & Signaling
ko03030 ko03410 ko03420 ko03430 map03030 map03410 map03420 map03430
Pfam Domains
Protein Families
DNA_ligase_A_C DNA_ligase_A_M DNA_ligase_A_N DRMBL

Protein Analysis

1403

Amino Acids

156.06

Weight (kDa)

6.38

Isoelectric Point (pI)

44.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DRMBL PF07522 269 - 372 2.4e-20 DNA repair metallo-beta-lactamase
DNA_ligase_A_N PF04675 772 - 948 6.9e-43 DNA ligase N terminus
DNA_ligase_A_M PF01068 1018 - 1238 1.3e-46 ATP dependent DNA ligase domain
DNA_ligase_A_C PF04679 1264 - 1371 3.2e-16 ATP dependent DNA ligase C terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015031)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66730
fragaria_vesca FvH4_1g29000 FvH4_1g29000 FvH4_1g29000
malus_domestica MD00G1054100.v1.1
prunus_persica Prupe.3G150300_v2.0.a1
pyrus_communis pycom17g25560
rosa_chinensis RchiOBHm_Chr3g0493691 RchiOBHm_Chr3g0493951
rosa_laevigata RLG00000022694
rosa_roxburghii Rroxscaffold_6G00391370
rosa_rugosa Rorug03G0263400 Rorug03G0263500 Rorug03G0263600
rosa_samantha Rh3AG312500 Rh3AG313200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 312
Acc36I ACCTGC 3 cut(s) 1634, 2067, 2842
Acc65I GGTACC 1 cut(s) 2006
AccB1I GGYRCC 3 cut(s) 413, 2006, 3964
AccB7I CCANNNNNTGG 1 cut(s) 4070
AccBSI CCGCTC 2 cut(s) 293, 349
AccIII TCCGGA 1 cut(s) 2676
AciI CCGC 8 cut(s) 57, 178, 240, 291, 347, 441, 773, 3469
AclI AACGTT 1 cut(s) 3383
AclWI GGATC 4 cut(s) 1015, 1299, 1888, 2544
AcoI YGGCCR 4 cut(s) 875, 1191, 3139, 3930
AcsI RAATTY 8 cut(s) 578, 892, 1535, 1556, 1798, 2860, 3153, 3842
AcuI CTGAAG 6 cut(s) 1299, 1949, 2424, 2900, 3113, 3858
AcvI CACGTG 2 cut(s) 3353, 4070
AcyI GRCGYC 4 cut(s) 309, 396, 414, 543
AdeI CACNNNGTG 1 cut(s) 1619
AfaI GTAC 9 cut(s) 1032, 2008, 2211, 2570, 2691, 2701, 2955, 3691, 3800
AfiI CCNNNNNNNGG 5 cut(s) 255, 1112, 1284, 2559, 4070
AflIII ACRYGT 1 cut(s) 3119
AhlI ACTAGT 1 cut(s) 3163
AjnI CCWGG 4 cut(s) 935, 1732, 3034, 4038
AjuI GAANNNNNNNTTGG 4 cut(s) 440, 472, 2481, 2513
AleI CACNNNNGTG 1 cut(s) 477
AloI GAACNNNNNNTCC 2 cut(s) 90, 122
Alw21I GWGCWC 4 cut(s) 1505, 2456, 2716, 3151
Alw44I GTGCAC 2 cut(s) 2452, 3147
AlwI GGATC 4 cut(s) 1015, 1299, 1888, 2544
AlwNI CAGNNNCTG 4 cut(s) 1649, 2702, 2933, 4145
Ama87I CYCGRG 1 cut(s) 409
Aor13HI TCCGGA 1 cut(s) 2676
AoxI GGCC 8 cut(s) 265, 875, 887, 1146, 1191, 1808, 3139, 3930
ApaLI GTGCAC 2 cut(s) 2452, 3147
ApeKI GCWGC 8 cut(s) 778, 2237, 2434, 2468, 2519, 2930, 4043, 4206
ApoI RAATTY 8 cut(s) 578, 892, 1535, 1556, 1798, 2860, 3153, 3842
ArsI GACNNNNNNTTYG 6 cut(s) 2608, 2640, 3416, 3448, 3841, 3873
AseI ATTAAT 1 cut(s) 3234
Asp700I GAANNNNTTC 4 cut(s) 1434, 2864, 3843, 4161
Asp718I GGTACC 1 cut(s) 2006
AspLEI GCGC 2 cut(s) 416, 2922
AspS9I GGNCC 4 cut(s) 34, 258, 1808, 3174
AsuC2I CCSGG 2 cut(s) 410, 411
AsuII TTCGAA 1 cut(s) 2794
AvaI CYCGRG 1 cut(s) 409
AvaII GGWCC 3 cut(s) 34, 258, 3174
BaeGI GKGCMC 2 cut(s) 2456, 3151
BaeI ACNNNNGTAYC 4 cut(s) 1329, 1362, 2788, 2821
BalI TGGCCA 4 cut(s) 877, 1193, 3141, 3932
BanI GGYRCC 3 cut(s) 413, 2006, 3964
BanII GRGCYC 2 cut(s) 1118, 1505
BarI GAAGNNNNNNTAC 4 cut(s) 3503, 3535, 4126, 4158
BbrPI CACGTG 2 cut(s) 3353, 4070
BbsI GAAGAC 2 cut(s) 290, 3412
Bbv12I GWGCWC 4 cut(s) 1505, 2456, 2716, 3151
BbvCI CCTCAGC 1 cut(s) 3960
BbvI GCAGC 7 cut(s) 765, 2224, 2421, 2455, 2531, 2942, 4030
BccI CCATC 9 cut(s) 365, 1032, 1970, 3131, 3164, 3688, 4072, 4177, 4196
BceAI ACGGC 6 cut(s) 33, 253, 401, 530, 1444, 1574
BciT130I CCWGG 4 cut(s) 937, 1734, 3036, 4040
BciVI GTATCC 2 cut(s) 550, 1609
BclI TGATCA 1 cut(s) 1564
BcnI CCSGG 2 cut(s) 410, 411
BcuI ACTAGT 1 cut(s) 3163
BfaI CTAG 7 cut(s) 23, 509, 621, 1487, 2348, 3164, 4055
BfmI CTRYAG 8 cut(s) 1036, 1107, 1211, 1233, 2277, 2469, 2892, 2934
BfoI RGCGCY 2 cut(s) 417, 2923
BfuAI ACCTGC 3 cut(s) 1634, 2067, 2842
BfuI GTATCC 2 cut(s) 550, 1609
BglII AGATCT 4 cut(s) 1489, 2584, 3226, 3924
BisI GCNGC 9 cut(s) 347, 779, 2238, 2435, 2469, 2520, 2931, 4044, 4207
BlsI GCNGC 9 cut(s) 348, 780, 2239, 2436, 2470, 2521, 2932, 4045, 4208
Bme1390I CCNGG 6 cut(s) 410, 411, 937, 1734, 3036, 4040
Bme18I GGWCC 3 cut(s) 34, 258, 3174
BmeT110I CYCGRG 1 cut(s) 409
BmgT120I GGNCC 4 cut(s) 34, 258, 1808, 3174
BmiI GGNNCC 8 cut(s) 260, 339, 415, 1117, 2008, 3033, 3175, 3966
BmrFI CCNGG 6 cut(s) 410, 411, 937, 1734, 3036, 4040
BpiI GAAGAC 2 cut(s) 290, 3412
BplI GAGNNNNNCTC 2 cut(s) 1595, 1627
BpmI CTGGAG 3 cut(s) 2314, 2546, 4014
Bpu10I CCTNAGC 3 cut(s) 1504, 3960, 3987
Bpu14I TTCGAA 1 cut(s) 2794
BpuEI CTTGAG 2 cut(s) 2586, 2829
BpuMI CCSGG 2 cut(s) 410, 411
Bsa29I ATCGAT 1 cut(s) 369
BsaAI YACGTR 3 cut(s) 2804, 3353, 4070
BsaHI GRCGYC 4 cut(s) 309, 396, 414, 543
BsaI GGTCTC 2 cut(s) 248, 1598
BsaJI CCNNGG 6 cut(s) 262, 409, 936, 1278, 1732, 3534
BsaWI WCCGGW 1 cut(s) 2676
BsaXI ACNNNNNCTCC 6 cut(s) 90, 120, 209, 239, 4012, 4042
Bsc4I CCNNNNNNNGG 5 cut(s) 255, 1112, 1284, 2559, 4070
Bse118I RCCGGY 2 cut(s) 185, 2610
Bse1I ACTGG 8 cut(s) 481, 964, 1149, 2529, 2707, 3285, 3573, 4031
Bse3DI GCAATG 4 cut(s) 403, 1987, 2305, 2820
BseAI TCCGGA 1 cut(s) 2676
BseBI CCWGG 4 cut(s) 937, 1734, 3036, 4040
BseCI ATCGAT 1 cut(s) 369
BseDI CCNNGG 6 cut(s) 262, 409, 936, 1278, 1732, 3534
BseLI CCNNNNNNNGG 5 cut(s) 255, 1112, 1284, 2559, 4070
BseMI GCAATG 4 cut(s) 403, 1987, 2305, 2820
BseMII CTCAG 4 cut(s) 1518, 3951, 3978, 4184
BseNI ACTGG 8 cut(s) 481, 964, 1149, 2529, 2707, 3285, 3573, 4031
BseRI GAGGAG 4 cut(s) 923, 1362, 2628, 3001
BseSI GKGCMC 2 cut(s) 2456, 3151
BseXI GCAGC 7 cut(s) 765, 2224, 2421, 2455, 2531, 2942, 4030
BseYI CCCAGC 1 cut(s) 1172
BsgI GTGCAG 3 cut(s) 1890, 2655, 4032
Bsh1285I CGRYCG 1 cut(s) 1546
BshFI GGCC 8 cut(s) 267, 877, 889, 1148, 1193, 1810, 3141, 3932
BshNI GGYRCC 3 cut(s) 413, 2006, 3964
BshVI ATCGAT 1 cut(s) 369
BsiEI CGRYCG 1 cut(s) 1546
BsiHKAI GWGCWC 4 cut(s) 1505, 2456, 2716, 3151
BsiHKCI CYCGRG 1 cut(s) 409
BsiSI CCGG 4 cut(s) 186, 410, 2611, 2677
BslFI GGGAC 8 cut(s) 75, 244, 938, 1243, 1526, 2554, 3740, 3932
BslI CCNNNNNNNGG 5 cut(s) 255, 1112, 1284, 2559, 4070
BsmBI CGTCTC 2 cut(s) 205, 325
BsmFI GGGAC 8 cut(s) 75, 244, 938, 1243, 1526, 2554, 3740, 3932
BsmI GAATGC 2 cut(s) 173, 1045
BsnI GGCC 8 cut(s) 267, 877, 889, 1148, 1193, 1810, 3141, 3932
Bso31I GGTCTC 2 cut(s) 248, 1598
BsoBI CYCGRG 1 cut(s) 409
Bsp119I TTCGAA 1 cut(s) 2794
Bsp1286I GDGCHC 5 cut(s) 1118, 1505, 2456, 2716, 3151
Bsp13I TCCGGA 1 cut(s) 2676
Bsp1407I TGTACA 2 cut(s) 2568, 2689
Bsp19I CCATGG 1 cut(s) 1278
BspACI CCGC 8 cut(s) 57, 178, 240, 291, 347, 441, 773, 3469
BspANI GGCC 8 cut(s) 267, 877, 889, 1148, 1193, 1810, 3141, 3932
BspCNI CTCAG 4 cut(s) 1517, 3952, 3979, 4183
BspDI ATCGAT 1 cut(s) 369
BspEI TCCGGA 1 cut(s) 2676
BspHI TCATGA 2 cut(s) 1561, 2988
BspLI GGNNCC 8 cut(s) 260, 339, 415, 1117, 2008, 3033, 3175, 3966
BspMAI CTGCAG 2 cut(s) 1215, 2473
BspMI ACCTGC 3 cut(s) 1634, 2067, 2842
BspPI GGATC 4 cut(s) 1015, 1299, 1888, 2544
BspT104I TTCGAA 1 cut(s) 2794
BspT107I GGYRCC 3 cut(s) 413, 2006, 3964
BspTNI GGTCTC 2 cut(s) 248, 1598
BsrBI CCGCTC 2 cut(s) 293, 349
BsrDI GCAATG 4 cut(s) 403, 1987, 2305, 2820
BsrFI RCCGGY 2 cut(s) 185, 2610
BsrGI TGTACA 2 cut(s) 2568, 2689
BsrI ACTGG 8 cut(s) 481, 964, 1149, 2529, 2707, 3285, 3573, 4031
BssAI RCCGGY 2 cut(s) 185, 2610
BssECI CCNNGG 6 cut(s) 262, 409, 936, 1278, 1732, 3534
BssNI GRCGYC 4 cut(s) 309, 396, 414, 543
BssT1I CCWWGG 3 cut(s) 262, 1278, 3534
Bst2UI CCWGG 4 cut(s) 937, 1734, 3036, 4040
Bst6I CTCTTC 3 cut(s) 44, 328, 2155
BstACI GRCGYC 4 cut(s) 309, 396, 414, 543
BstAPI GCANNNNNTGC 1 cut(s) 3560
BstAUI TGTACA 2 cut(s) 2568, 2689
BstBAI YACGTR 3 cut(s) 2804, 3353, 4070
BstBI TTCGAA 1 cut(s) 2794
BstDEI CTNAG 5 cut(s) 1064, 1504, 3960, 3987, 4170
BstDSI CCRYGG 1 cut(s) 1278
BstH2I RGCGCY 2 cut(s) 417, 2923
BstHHI GCGC 2 cut(s) 416, 2922
BstMCI CGRYCG 1 cut(s) 1546
BstMWI GCNNNNNNNGC 6 cut(s) 56, 170, 186, 355, 3475, 3560
BstNI CCWGG 4 cut(s) 937, 1734, 3036, 4040
BstNSI RCATGY 8 cut(s) 845, 2292, 2312, 2538, 2610, 3123, 3185, 3822
BstSCI CCNGG 6 cut(s) 408, 409, 935, 1732, 3034, 4038
BstSFI CTRYAG 8 cut(s) 1036, 1107, 1211, 1233, 2277, 2469, 2892, 2934
BstSLI GKGCMC 2 cut(s) 2456, 3151
BstSNI TACGTA 1 cut(s) 2804
BstV1I GCAGC 7 cut(s) 765, 2224, 2421, 2455, 2531, 2942, 4030
BstV2I GAAGAC 2 cut(s) 290, 3412
BstX2I RGATCY 6 cut(s) 1020, 1489, 2549, 2584, 3226, 3924
BstYI RGATCY 6 cut(s) 1020, 1489, 2549, 2584, 3226, 3924
Bsu15I ATCGAT 1 cut(s) 369
BsuI GTATCC 2 cut(s) 550, 1609
BsuRI GGCC 8 cut(s) 267, 877, 889, 1148, 1193, 1810, 3141, 3932
BsuTUI ATCGAT 1 cut(s) 369
BtgI CCRYGG 1 cut(s) 1278
BtsI GCAGTG 2 cut(s) 2716, 3273
BveI ACCTGC 3 cut(s) 1634, 2067, 2842
CaiI CAGNNNCTG 4 cut(s) 1649, 2702, 2933, 4145
CciI TCATGA 2 cut(s) 1561, 2988
CfoI GCGC 2 cut(s) 416, 2922
Cfr10I RCCGGY 2 cut(s) 185, 2610
Cfr13I GGNCC 4 cut(s) 34, 258, 1808, 3174
Cfr9I CCCGGG 1 cut(s) 409
ClaI ATCGAT 1 cut(s) 369
CseI GACGC 6 cut(s) 179, 404, 551, 2540, 3400, 3841
Csp6I GTAC 9 cut(s) 1031, 2007, 2210, 2569, 2690, 2700, 2954, 3690, 3799
CviQI GTAC 9 cut(s) 1031, 2007, 2210, 2569, 2690, 2700, 2954, 3690, 3799
DdeI CTNAG 5 cut(s) 1064, 1504, 3960, 3987, 4170
DinI GGCGCC 1 cut(s) 415
DraIII CACNNNGTG 1 cut(s) 1619
EaeI YGGCCR 4 cut(s) 875, 1191, 3139, 3930
Eam1104I CTCTTC 3 cut(s) 44, 328, 2155
EarI CTCTTC 3 cut(s) 44, 328, 2155
Ecl136II GAGCTC 1 cut(s) 1503
Eco105I TACGTA 1 cut(s) 2804
Eco130I CCWWGG 3 cut(s) 262, 1278, 3534
Eco147I AGGCCT 1 cut(s) 267
Eco24I GRGCYC 2 cut(s) 1118, 1505
Eco31I GGTCTC 2 cut(s) 248, 1598
Eco47I GGWCC 3 cut(s) 34, 258, 3174
Eco53kI GAGCTC 1 cut(s) 1503
Eco57I CTGAAG 6 cut(s) 1299, 1949, 2424, 2900, 3113, 3858
Eco72I CACGTG 2 cut(s) 3353, 4070
Eco88I CYCGRG 1 cut(s) 409
EcoICRI GAGCTC 1 cut(s) 1503
EcoRI GAATTC 1 cut(s) 2860
EcoRII CCWGG 4 cut(s) 935, 1732, 3034, 4038
EcoT14I CCWWGG 3 cut(s) 262, 1278, 3534
EcoT22I ATGCAT 4 cut(s) 2290, 3183, 3376, 3619
EcoT38I GRGCYC 2 cut(s) 1118, 1505
EgeI GGCGCC 1 cut(s) 415
EheI GGCGCC 1 cut(s) 415
ErhI CCWWGG 3 cut(s) 262, 1278, 3534
Esp3I CGTCTC 2 cut(s) 205, 325
FaqI GGGAC 8 cut(s) 75, 244, 938, 1243, 1526, 2554, 3740, 3932
FauI CCCGC 1 cut(s) 448
FauNDI CATATG 1 cut(s) 691
FbaI TGATCA 1 cut(s) 1564
Fnu4HI GCNGC 9 cut(s) 347, 779, 2238, 2435, 2469, 2520, 2931, 4044, 4207
FriOI GRGCYC 2 cut(s) 1118, 1505
Fsp4HI GCNGC 9 cut(s) 347, 779, 2238, 2435, 2469, 2520, 2931, 4044, 4207
FspBI CTAG 7 cut(s) 23, 509, 621, 1487, 2348, 3164, 4055
GlaI GCGC 2 cut(s) 415, 2921
GluI GCNGC 9 cut(s) 347, 779, 2238, 2435, 2469, 2520, 2931, 4044, 4207
GsaI CCCAGC 1 cut(s) 1176
GsuI CTGGAG 3 cut(s) 2314, 2546, 4014
HaeII RGCGCY 2 cut(s) 417, 2923
HaeIII GGCC 8 cut(s) 267, 877, 889, 1148, 1193, 1810, 3141, 3932
HapII CCGG 4 cut(s) 186, 410, 2611, 2677
HgaI GACGC 6 cut(s) 179, 404, 551, 2540, 3400, 3841
HhaI GCGC 2 cut(s) 416, 2922
Hin1I GRCGYC 4 cut(s) 309, 396, 414, 543
Hin6I GCGC 2 cut(s) 414, 2920
HinP1I GCGC 2 cut(s) 414, 2920
HincII GTYRAC 5 cut(s) 1255, 1410, 1768, 3210, 3403
HindII GTYRAC 5 cut(s) 1255, 1410, 1768, 3210, 3403
HindIII AAGCTT 3 cut(s) 2912, 3111, 3457
HpaI GTTAAC 2 cut(s) 1410, 1768
HpaII CCGG 4 cut(s) 186, 410, 2611, 2677
Hpy99I CGWCG 2 cut(s) 173, 2237
HpyCH4IV ACGT 9 cut(s) 309, 318, 1074, 1857, 2232, 2803, 3352, 3383, 4069
HpyF10VI GCNNNNNNNGC 6 cut(s) 56, 170, 186, 355, 3475, 3560
HpyF3I CTNAG 5 cut(s) 1064, 1504, 3960, 3987, 4170
HpySE526I ACGT 9 cut(s) 309, 318, 1074, 1857, 2232, 2803, 3352, 3383, 4069
Hsp92I GRCGYC 4 cut(s) 309, 396, 414, 543
HspAI GCGC 2 cut(s) 414, 2920
KasI GGCGCC 1 cut(s) 413
Kpn2I TCCGGA 1 cut(s) 2676
KpnI GGTACC 1 cut(s) 2010
KroI GCCGGC 2 cut(s) 185, 2610
KroNI GCCGGC 2 cut(s) 187, 2612
Ksp22I TGATCA 1 cut(s) 1564
KspAI GTTAAC 2 cut(s) 1410, 1768
Lsp1109I GCAGC 7 cut(s) 765, 2224, 2421, 2455, 2531, 2942, 4030
MaeI CTAG 7 cut(s) 23, 509, 621, 1487, 2348, 3164, 4055
MaeII ACGT 9 cut(s) 309, 318, 1074, 1857, 2232, 2803, 3352, 3383, 4069
MbiI CCGCTC 2 cut(s) 293, 349
MfeI CAATTG 2 cut(s) 253, 3759
MflI RGATCY 6 cut(s) 1020, 1489, 2549, 2584, 3226, 3924
MhlI GDGCHC 5 cut(s) 1118, 1505, 2456, 2716, 3151
MlsI TGGCCA 4 cut(s) 877, 1193, 3141, 3932
MluNI TGGCCA 4 cut(s) 877, 1193, 3141, 3932
Mly113I GGCGCC 1 cut(s) 414
MlyI GAGTC 2 cut(s) 645, 3215
MmeI TCCRAC 3 cut(s) 619, 3277, 3366
Mox20I TGGCCA 4 cut(s) 877, 1193, 3141, 3932
Mph1103I ATGCAT 4 cut(s) 2290, 3183, 3376, 3619
MroI TCCGGA 1 cut(s) 2676
MroNI GCCGGC 2 cut(s) 185, 2610
MroXI GAANNNNTTC 4 cut(s) 1434, 2864, 3843, 4161
MscI TGGCCA 4 cut(s) 877, 1193, 3141, 3932
MslI CAYNNNNRTG 2 cut(s) 477, 2481
Msp20I TGGCCA 4 cut(s) 877, 1193, 3141, 3932
MspA1I CMGCKG 3 cut(s) 2933, 3959, 4209
MspI CCGG 4 cut(s) 186, 410, 2611, 2677
MspR9I CCNGG 6 cut(s) 410, 411, 937, 1734, 3036, 4040
MunI CAATTG 2 cut(s) 253, 3759
Mva1269I GAATGC 2 cut(s) 173, 1045
MvaI CCWGG 4 cut(s) 937, 1734, 3036, 4040
MwoI GCNNNNNNNGC 6 cut(s) 56, 170, 186, 355, 3475, 3560
NaeI GCCGGC 2 cut(s) 187, 2612
NarI GGCGCC 1 cut(s) 414
NciI CCSGG 2 cut(s) 410, 411
NcoI CCATGG 1 cut(s) 1278
NdeI CATATG 1 cut(s) 691
NgoMIV GCCGGC 2 cut(s) 185, 2610
NlaIV GGNNCC 8 cut(s) 260, 339, 415, 1117, 2008, 3033, 3175, 3966
NmuCI GTSAC 8 cut(s) 1468, 1528, 1619, 1927, 2250, 2512, 3268, 4108
NsiI ATGCAT 4 cut(s) 2290, 3183, 3376, 3619
NspI RCATGY 8 cut(s) 845, 2292, 2312, 2538, 2610, 3123, 3185, 3822
NspV TTCGAA 1 cut(s) 2794
OliI CACNNNNGTG 1 cut(s) 477
PaeI GCATGC 2 cut(s) 2292, 2610
PagI TCATGA 2 cut(s) 1561, 2988
PceI AGGCCT 1 cut(s) 267
PciI ACATGT 1 cut(s) 3119
PcsI WCGNNNNNNNCGW 3 cut(s) 315, 375, 2800
PctI GAATGC 2 cut(s) 173, 1045
PdiI GCCGGC 2 cut(s) 187, 2612
PdmI GAANNNNTTC 4 cut(s) 1434, 2864, 3843, 4161
PfeI GAWTC 9 cut(s) 26, 602, 1102, 1388, 1985, 2737, 3006, 3066, 4086
PflMI CCANNNNNTGG 1 cut(s) 4070
PfoI TCCNGGA 1 cut(s) 3034
PkrI GCNGC 9 cut(s) 348, 780, 2239, 2436, 2470, 2521, 2932, 4045, 4208
Ple19I CGATCG 1 cut(s) 1546
PleI GAGTC 2 cut(s) 644, 3214
PluTI GGCGCC 1 cut(s) 417
PmaCI CACGTG 2 cut(s) 3353, 4070
PmlI CACGTG 2 cut(s) 3353, 4070
PpsI GAGTC 2 cut(s) 644, 3214
Ppu21I YACGTR 3 cut(s) 2804, 3353, 4070
PscI ACATGT 1 cut(s) 3119
PshBI ATTAAT 1 cut(s) 3234
Psp124BI GAGCTC 1 cut(s) 1505
Psp1406I AACGTT 1 cut(s) 3383
Psp6I CCWGG 4 cut(s) 935, 1732, 3034, 4038
PspCI CACGTG 2 cut(s) 3353, 4070
PspFI CCCAGC 1 cut(s) 1172
PspGI CCWGG 4 cut(s) 935, 1732, 3034, 4038
PspN4I GGNNCC 8 cut(s) 260, 339, 415, 1117, 2008, 3033, 3175, 3966
PspPI GGNCC 4 cut(s) 34, 258, 1808, 3174
PstI CTGCAG 2 cut(s) 1215, 2473
PstNI CAGNNNCTG 4 cut(s) 1649, 2702, 2933, 4145
PsuI RGATCY 6 cut(s) 1020, 1489, 2549, 2584, 3226, 3924
PvuI CGATCG 1 cut(s) 1546
PvuII CAGCTG 3 cut(s) 2933, 3959, 4209
RsaI GTAC 9 cut(s) 1032, 2008, 2211, 2570, 2691, 2701, 2955, 3691, 3800
RsaNI GTAC 9 cut(s) 1031, 2007, 2210, 2569, 2690, 2700, 2954, 3690, 3799
RseI CAYNNNNRTG 2 cut(s) 477, 2481
SacI GAGCTC 1 cut(s) 1505
SatI GCNGC 9 cut(s) 347, 779, 2238, 2435, 2469, 2520, 2931, 4044, 4207
Sau96I GGNCC 4 cut(s) 34, 258, 1808, 3174
SbfI CCTGCAGG 1 cut(s) 1215
SchI GAGTC 2 cut(s) 645, 3215
ScrFI CCNGG 6 cut(s) 410, 411, 937, 1734, 3036, 4040
SdaI CCTGCAGG 1 cut(s) 1215
SduI GDGCHC 5 cut(s) 1118, 1505, 2456, 2716, 3151
SfcI CTRYAG 8 cut(s) 1036, 1107, 1211, 1233, 2277, 2469, 2892, 2934
SfoI GGCGCC 1 cut(s) 415
SfuI TTCGAA 1 cut(s) 2794
SinI GGWCC 3 cut(s) 34, 258, 3174
SmaI CCCGGG 1 cut(s) 411
SmiMI CAYNNNNRTG 2 cut(s) 477, 2481
SmlI CTYRAG 2 cut(s) 2601, 2844
SmoI CTYRAG 2 cut(s) 2601, 2844
SnaBI TACGTA 1 cut(s) 2804
SpeI ACTAGT 1 cut(s) 3163
SphI GCATGC 2 cut(s) 2292, 2610
SrfI GCCCGGGC 1 cut(s) 411
Sse8387I CCTGCAGG 1 cut(s) 1215
SseBI AGGCCT 1 cut(s) 267
SsiI CCGC 8 cut(s) 57, 178, 240, 291, 347, 441, 773, 3469
SspDI GGCGCC 1 cut(s) 413
SspI AATATT 3 cut(s) 1404, 1663, 1711
SspMI CTAG 7 cut(s) 23, 509, 621, 1487, 2348, 3164, 4055
SstI GAGCTC 1 cut(s) 1505
StuI AGGCCT 1 cut(s) 267
StyD4I CCNGG 6 cut(s) 408, 409, 935, 1732, 3034, 4038
StyI CCWWGG 3 cut(s) 262, 1278, 3534
TaiI ACGT 9 cut(s) 312, 321, 1077, 1860, 2235, 2806, 3355, 3386, 4072
TaqI TCGA 9 cut(s) 168, 369, 763, 1820, 2716, 2794, 3215, 3423, 4084
TatI WGTACW 3 cut(s) 2568, 2689, 2953
TauI GCSGC 1 cut(s) 349
TfiI GAWTC 9 cut(s) 26, 602, 1102, 1388, 1985, 2737, 3006, 3066, 4086
TseFI GTSAC 8 cut(s) 1468, 1528, 1619, 1927, 2250, 2512, 3268, 4108
TseI GCWGC 8 cut(s) 778, 2237, 2434, 2468, 2519, 2930, 4043, 4206
Tsp45I GTSAC 8 cut(s) 1468, 1528, 1619, 1927, 2250, 2512, 3268, 4108
TspGWI ACGGA 4 cut(s) 47, 187, 512, 810
TspMI CCCGGG 1 cut(s) 409
Van91I CCANNNNNTGG 1 cut(s) 4070
VneI GTGCAC 2 cut(s) 2452, 3147
VpaK11BI GGWCC 3 cut(s) 34, 258, 3174
VspI ATTAAT 1 cut(s) 3234
XapI RAATTY 8 cut(s) 578, 892, 1535, 1556, 1798, 2860, 3153, 3842
XbaI TCTAGA 1 cut(s) 22
XceI RCATGY 8 cut(s) 845, 2292, 2312, 2538, 2610, 3123, 3185, 3822
XcmI CCANNNNNNNNNTGG 2 cut(s) 2968, 3765
XmaI CCCGGG 1 cut(s) 409
XmnI GAANNNNTTC 4 cut(s) 1434, 2864, 3843, 4161
XspI CTAG 7 cut(s) 23, 509, 621, 1487, 2348, 3164, 4055
ZraI GACGTC 1 cut(s) 310
Zsp2I ATGCAT 4 cut(s) 2290, 3183, 3376, 3619
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.