RchiOBHm_Chr3g0493951

DNA ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0015031
Physical Location & Seq
Reverse (-)
41518272 .. 41519714
1443 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45663

Sequence Viewer

Length: 1182 bp
ATGACGGCTCTGGATTCTACGGACCTCTTCCTGACTGCCCTAACCGCCTTTTCCCACCCACCCAGACCCATACCCCGTCCCCTTGAACTCTCTCTCCCTCCAATTCCCTCCACATTCCCCCACTCCAAACTCATCCCCAGAACCCGTTTCGCCGTCGATGCATTCCGCTATGCCGGCGATTATTCCGTCTCTTACTTCCTCTCTCACTTCCACTCCGATCACTACGGCGGTCTCTCCCCCAATTGGTCCCAAGGCCTCGTCTTTTGCTCCCCCACCACCGCTCGTCTTCTCACAGACGTCCTCAACGTCTCTTCGCTTTTCGTGGTTCCTCTGCCGCTCGGACAGCCCCTTCTCATCGATGGATGCGAGGTCGTGCTTATTGACGCCAATCATTGCCCGGGCGCCGTTCAGTTCTTGTTCAAAGTTCCCGCCTCCAATGGAGACTTCCAGAGGTATGTTCACACTGGTGATTTTCGGTTTTCTGCTCCGATGAAGCTAGACCCTTTTCTCTGTAACTTTGTGGGTTCAGACGCCGTTTTCTTGGATACAACTTATTGCAATCCCAAATTCGTGTTTCCTTCACAAGAAGAATCTGTTGATTATATTTCTAGCTTGATAGAAAGAGTTGGAGGTGAACATGAAGTTTCTGTGAAAAGTGTTCTGTTCCTTGTTGCTACATATGTGATTGGGAAAGAGAAGATTTTGATAGAAATTGCTCGTAGATGTAAGCGTAAAGTGTATGTGGATGCTCGAAAAATGGCGGTGCTGCGTGTTTTGGGTTACGGAGACAGTGGGGTGTTTACGGAGGATGAATGTGAGAGTGATGTGCATGTTGTTGGGTGGAATGTGTTGGGTGAGACTTGGCCATATTTCAGGCCAAATTTTGTGAAAATGGAGGAGATTATGGTTGAGAGGGGGTATTCCAGGGTTGTAGGGTTTGTCCCTACTGGCTGGACTTATGAAGTCAAGCGTAACAAGTTCTCGGTGAGGTCAAAGGATGCTTTTGAGATCCATCTTGTACCCTACAGTGAGCATTCAAGCTATGATGAACTTAGAGAATACGTCAGGTTTTTGAAACCAAGGCGTGTGATTCCTACAGTGGGCTCCGATGTGGAAAAGATAGACAGTAAACATGCCAGTAAAATGCAAAAGCATTTTGCTGGGTTAATAATTAACCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000012 GO:0000726 GO:0002237 GO:0003006 GO:0003674 GO:0003824 GO:0003909 GO:0003910 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006266 GO:0006271 GO:0006273 GO:0006281 GO:0006302 GO:0006303 GO:0006304 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009314 GO:0009409 GO:0009411 GO:0009416 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009791 GO:0009845 GO:0009987 GO:0010033 GO:0010154 GO:0010225 GO:0010243 GO:0015074 GO:0016070 GO:0016874 GO:0016886 GO:0022414 GO:0022616 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0033554 GO:0034641 GO:0034645 GO:0035510 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044728 GO:0046483 GO:0046677 GO:0048316 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051103 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0065007 GO:0070013 GO:0070988 GO:0071704 GO:0080111 GO:0090304 GO:0090351 GO:0140097 GO:1901360 GO:1901576 GO:1901652 GO:1901698 GO:1901700 GO:1904975 GO:2000683 GO:2000685
KEGG Pathways
Metabolic & Signaling
ko03030 ko03410 ko03420 ko03430 map03030 map03410 map03420 map03430
Pfam Domains
Protein Families
DNA_ligase_A_C DNA_ligase_A_M DNA_ligase_A_N DRMBL

Protein Analysis

393

Amino Acids

44.46

Weight (kDa)

6.89

Isoelectric Point (pI)

37.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DRMBL PF07522 265 - 368 1.1e-21 DNA repair metallo-beta-lactamase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015031)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66730
fragaria_vesca FvH4_1g29000 FvH4_1g29000 FvH4_1g29000
malus_domestica MD00G1054100.v1.1
prunus_persica Prupe.3G150300_v2.0.a1
pyrus_communis pycom17g25560
rosa_chinensis RchiOBHm_Chr3g0493691 RchiOBHm_Chr3g0493951
rosa_laevigata RLG00000022694
rosa_roxburghii Rroxscaffold_6G00391370
rosa_rugosa Rorug03G0263400 Rorug03G0263500 Rorug03G0263600
rosa_samantha Rh3AG312500 Rh3AG313200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 300
AccB1I GGYRCC 1 cut(s) 401
AccBSI CCGCTC 2 cut(s) 281, 337
AciI CCGC 7 cut(s) 45, 166, 228, 279, 335, 429, 761
AclWI GGATC 1 cut(s) 1003
AcoI YGGCCR 1 cut(s) 863
AcsI RAATTY 2 cut(s) 566, 880
AcyI GRCGYC 4 cut(s) 297, 384, 402, 531
AfaI GTAC 1 cut(s) 1020
AfiI CCNNNNNNNGG 2 cut(s) 243, 1100
AgsI TTSAA 4 cut(s) 86, 421, 1038, 1075
AjnI CCWGG 1 cut(s) 923
AjuI GAANNNNNNNTTGG 2 cut(s) 428, 460
AleI CACNNNNGTG 1 cut(s) 465
AloI GAACNNNNNNTCC 2 cut(s) 78, 110
AluBI AGCT 3 cut(s) 496, 612, 1041
AluI AGCT 3 cut(s) 496, 612, 1041
Alw26I GTCTC 6 cut(s) 193, 236, 313, 435, 780, 851
AlwI GGATC 1 cut(s) 1003
Ama87I CYCGRG 1 cut(s) 397
AoxI GGCC 3 cut(s) 253, 863, 875
ApeKI GCWGC 1 cut(s) 766
ApoI RAATTY 2 cut(s) 566, 880
AspLEI GCGC 1 cut(s) 404
AspS9I GGNCC 2 cut(s) 22, 246
AsuC2I CCSGG 2 cut(s) 398, 399
AsuHPI GGTGA 4 cut(s) 479, 644, 866, 997
AvaI CYCGRG 1 cut(s) 397
AvaII GGWCC 2 cut(s) 22, 246
BalI TGGCCA 1 cut(s) 865
BanI GGYRCC 1 cut(s) 401
BanII GRGCYC 1 cut(s) 1106
BbsI GAAGAC 1 cut(s) 278
BbvI GCAGC 1 cut(s) 753
BccI CCATC 2 cut(s) 353, 1020
BceAI ACGGC 5 cut(s) 21, 137, 241, 389, 518
BciT130I CCWGG 1 cut(s) 925
BciVI GTATCC 1 cut(s) 538
BcnI CCSGG 2 cut(s) 398, 399
BcoDI GTCTC 6 cut(s) 193, 236, 313, 435, 780, 851
BfaI CTAG 2 cut(s) 497, 609
BfmI CTRYAG 2 cut(s) 1024, 1095
BfoI RGCGCY 1 cut(s) 405
BfuI GTATCC 1 cut(s) 538
BisI GCNGC 2 cut(s) 335, 767
BlsI GCNGC 2 cut(s) 336, 768
Bme1390I CCNGG 3 cut(s) 398, 399, 925
Bme18I GGWCC 2 cut(s) 22, 246
BmeT110I CYCGRG 1 cut(s) 397
BmgT120I GGNCC 2 cut(s) 22, 246
BmiI GGNNCC 4 cut(s) 248, 327, 403, 1105
BmrFI CCNGG 3 cut(s) 398, 399, 925
BmsI GCATC 4 cut(s) 148, 353, 736, 988
BpiI GAAGAC 1 cut(s) 278
BpuMI CCSGG 2 cut(s) 398, 399
Bsa29I ATCGAT 1 cut(s) 357
BsaHI GRCGYC 4 cut(s) 297, 384, 402, 531
BsaI GGTCTC 1 cut(s) 236
BsaJI CCNNGG 4 cut(s) 250, 397, 924, 1079
BsaXI ACNNNNNCTCC 4 cut(s) 78, 108, 197, 227
Bsc4I CCNNNNNNNGG 2 cut(s) 243, 1100
Bse118I RCCGGY 1 cut(s) 173
Bse1I ACTGG 3 cut(s) 469, 952, 1137
Bse3DI GCAATG 1 cut(s) 391
BseBI CCWGG 1 cut(s) 925
BseCI ATCGAT 1 cut(s) 357
BseDI CCNNGG 4 cut(s) 250, 397, 924, 1079
BseGI GGATG 5 cut(s) 132, 368, 751, 814, 1003
BseLI CCNNNNNNNGG 2 cut(s) 243, 1100
BseMI GCAATG 1 cut(s) 391
BseNI ACTGG 3 cut(s) 469, 952, 1137
BseRI GAGGAG 1 cut(s) 911
BseXI GCAGC 1 cut(s) 753
BseYI CCCAGC 1 cut(s) 1160
BshFI GGCC 3 cut(s) 255, 865, 877
BshNI GGYRCC 1 cut(s) 401
BshVI ATCGAT 1 cut(s) 357
BsiHKCI CYCGRG 1 cut(s) 397
BsiSI CCGG 2 cut(s) 174, 398
BslFI GGGAC 3 cut(s) 63, 232, 926
BslI CCNNNNNNNGG 2 cut(s) 243, 1100
BsmAI GTCTC 6 cut(s) 193, 236, 313, 435, 780, 851
BsmBI CGTCTC 2 cut(s) 193, 313
BsmFI GGGAC 3 cut(s) 63, 232, 926
BsmI GAATGC 2 cut(s) 161, 1033
BsnI GGCC 3 cut(s) 255, 865, 877
Bso31I GGTCTC 1 cut(s) 236
BsoBI CYCGRG 1 cut(s) 397
Bsp1286I GDGCHC 1 cut(s) 1106
Bsp143I GATC 2 cut(s) 217, 1008
BspACI CCGC 7 cut(s) 45, 166, 228, 279, 335, 429, 761
BspANI GGCC 3 cut(s) 255, 865, 877
BspDI ATCGAT 1 cut(s) 357
BspLI GGNNCC 4 cut(s) 248, 327, 403, 1105
BspPI GGATC 1 cut(s) 1003
BspT107I GGYRCC 1 cut(s) 401
BspTNI GGTCTC 1 cut(s) 236
BsrBI CCGCTC 2 cut(s) 281, 337
BsrDI GCAATG 1 cut(s) 391
BsrFI RCCGGY 1 cut(s) 173
BsrI ACTGG 3 cut(s) 469, 952, 1137
BssAI RCCGGY 1 cut(s) 173
BssECI CCNNGG 4 cut(s) 250, 397, 924, 1079
BssMI GATC 2 cut(s) 217, 1008
BssNI GRCGYC 4 cut(s) 297, 384, 402, 531
BssT1I CCWWGG 2 cut(s) 250, 1079
Bst2UI CCWGG 1 cut(s) 925
Bst4CI ACNGT 5 cut(s) 791, 1028, 1099, 1127, 1178
Bst6I CTCTTC 2 cut(s) 32, 316
BstACI GRCGYC 4 cut(s) 297, 384, 402, 531
BstC8I GCNNGC 1 cut(s) 175
BstDEI CTNAG 1 cut(s) 1052
BstF5I GGATG 5 cut(s) 132, 368, 751, 814, 1003
BstH2I RGCGCY 1 cut(s) 405
BstHHI GCGC 1 cut(s) 404
BstKTI GATC 2 cut(s) 220, 1011
BstMAI GTCTC 6 cut(s) 193, 236, 313, 435, 780, 851
BstMBI GATC 2 cut(s) 217, 1008
BstMWI GCNNNNNNNGC 4 cut(s) 44, 158, 174, 343
BstNI CCWGG 1 cut(s) 925
BstNSI RCATGY 2 cut(s) 833, 1136
BstSCI CCNGG 3 cut(s) 396, 397, 923
BstSFI CTRYAG 2 cut(s) 1024, 1095
BstV1I GCAGC 1 cut(s) 753
BstV2I GAAGAC 1 cut(s) 278
BstX2I RGATCY 1 cut(s) 1008
BstYI RGATCY 1 cut(s) 1008
Bsu15I ATCGAT 1 cut(s) 357
BsuI GTATCC 1 cut(s) 538
BsuRI GGCC 3 cut(s) 255, 865, 877
BsuTUI ATCGAT 1 cut(s) 357
BtsCI GGATG 5 cut(s) 132, 368, 751, 814, 1003
BtsIMutI CAGTG 4 cut(s) 462, 796, 1033, 1104
Cac8I GCNNGC 1 cut(s) 175
CfoI GCGC 1 cut(s) 404
Cfr10I RCCGGY 1 cut(s) 173
Cfr13I GGNCC 2 cut(s) 22, 246
Cfr9I CCCGGG 1 cut(s) 397
ClaI ATCGAT 1 cut(s) 357
CseI GACGC 2 cut(s) 392, 539
Csp6I GTAC 1 cut(s) 1019
CviAII CATG 3 cut(s) 638, 830, 1133
CviQI GTAC 1 cut(s) 1019
DdeI CTNAG 1 cut(s) 1052
DinI GGCGCC 1 cut(s) 403
DpnI GATC 2 cut(s) 219, 1010
DpnII GATC 2 cut(s) 217, 1008
EaeI YGGCCR 1 cut(s) 863
Eam1104I CTCTTC 2 cut(s) 32, 316
EarI CTCTTC 2 cut(s) 32, 316
Eco130I CCWWGG 2 cut(s) 250, 1079
Eco147I AGGCCT 1 cut(s) 255
Eco24I GRGCYC 1 cut(s) 1106
Eco31I GGTCTC 1 cut(s) 236
Eco47I GGWCC 2 cut(s) 22, 246
Eco88I CYCGRG 1 cut(s) 397
EcoRII CCWGG 1 cut(s) 923
EcoT14I CCWWGG 2 cut(s) 250, 1079
EcoT22I ATGCAT 1 cut(s) 163
EcoT38I GRGCYC 1 cut(s) 1106
EgeI GGCGCC 1 cut(s) 403
EheI GGCGCC 1 cut(s) 403
ErhI CCWWGG 2 cut(s) 250, 1079
Esp3I CGTCTC 2 cut(s) 193, 313
FaeI CATG 3 cut(s) 641, 833, 1136
FaqI GGGAC 3 cut(s) 63, 232, 926
FatI CATG 3 cut(s) 637, 829, 1132
FauI CCCGC 1 cut(s) 436
FauNDI CATATG 1 cut(s) 679
Fnu4HI GCNGC 2 cut(s) 335, 767
FokI GGATG 5 cut(s) 119, 375, 758, 821, 1010
FriOI GRGCYC 1 cut(s) 1106
Fsp4HI GCNGC 2 cut(s) 335, 767
FspBI CTAG 2 cut(s) 497, 609
GlaI GCGC 1 cut(s) 403
GluI GCNGC 2 cut(s) 335, 767
GsaI CCCAGC 1 cut(s) 1164
HaeII RGCGCY 1 cut(s) 405
HaeIII GGCC 3 cut(s) 255, 865, 877
HapII CCGG 2 cut(s) 174, 398
HgaI GACGC 2 cut(s) 392, 539
HhaI GCGC 1 cut(s) 404
Hin1I GRCGYC 4 cut(s) 297, 384, 402, 531
Hin1II CATG 3 cut(s) 641, 833, 1136
Hin6I GCGC 1 cut(s) 402
HinP1I GCGC 1 cut(s) 402
HinfI GANTC 3 cut(s) 14, 590, 1090
HpaII CCGG 2 cut(s) 174, 398
HphI GGTGA 4 cut(s) 479, 644, 866, 997
Hpy166II GTNNAC 4 cut(s) 460, 635, 801, 1130
Hpy188I TCNGA 5 cut(s) 217, 341, 489, 529, 1108
Hpy188III TCNNGA 3 cut(s) 11, 31, 448
Hpy8I GTNNAC 4 cut(s) 460, 635, 801, 1130
Hpy99I CGWCG 1 cut(s) 158
HpyAV CCTTC 2 cut(s) 359, 588
HpyCH4III ACNGT 5 cut(s) 791, 1028, 1099, 1127, 1178
HpyCH4IV ACGT 3 cut(s) 297, 306, 1062
HpyCH4V TGCA 4 cut(s) 161, 558, 829, 1147
HpyF10VI GCNNNNNNNGC 4 cut(s) 44, 158, 174, 343
HpyF3I CTNAG 1 cut(s) 1052
HpySE526I ACGT 3 cut(s) 297, 306, 1062
Hsp92I GRCGYC 4 cut(s) 297, 384, 402, 531
Hsp92II CATG 3 cut(s) 641, 833, 1136
HspAI GCGC 1 cut(s) 402
KasI GGCGCC 1 cut(s) 401
KroI GCCGGC 1 cut(s) 173
KroNI GCCGGC 1 cut(s) 175
Kzo9I GATC 2 cut(s) 217, 1008
LmnI GCTCC 3 cut(s) 272, 490, 1109
Lsp1109I GCAGC 1 cut(s) 753
LweI GCATC 4 cut(s) 148, 353, 736, 988
MaeI CTAG 2 cut(s) 497, 609
MaeII ACGT 3 cut(s) 297, 306, 1062
MaeIII GTNAC 3 cut(s) 512, 779, 971
MalI GATC 2 cut(s) 219, 1010
MbiI CCGCTC 2 cut(s) 281, 337
MboI GATC 2 cut(s) 217, 1008
MboII GAAGA 5 cut(s) 19, 278, 303, 599, 709
MfeI CAATTG 1 cut(s) 241
MflI RGATCY 1 cut(s) 1008
MhlI GDGCHC 1 cut(s) 1106
MlsI TGGCCA 1 cut(s) 865
MluCI AATT 6 cut(s) 102, 241, 566, 711, 880, 1170
MluNI TGGCCA 1 cut(s) 865
Mly113I GGCGCC 1 cut(s) 402
MmeI TCCRAC 1 cut(s) 607
Mox20I TGGCCA 1 cut(s) 865
Mph1103I ATGCAT 1 cut(s) 163
MroNI GCCGGC 1 cut(s) 173
MscI TGGCCA 1 cut(s) 865
MseI TTAA 3 cut(s) 1166, 1173, 1180
MslI CAYNNNNRTG 1 cut(s) 465
Msp20I TGGCCA 1 cut(s) 865
MspI CCGG 2 cut(s) 174, 398
MspR9I CCNGG 3 cut(s) 398, 399, 925
MunI CAATTG 1 cut(s) 241
Mva1269I GAATGC 2 cut(s) 161, 1033
MvaI CCWGG 1 cut(s) 925
MwoI GCNNNNNNNGC 4 cut(s) 44, 158, 174, 343
NaeI GCCGGC 1 cut(s) 175
NarI GGCGCC 1 cut(s) 402
NciI CCSGG 2 cut(s) 398, 399
NdeI CATATG 1 cut(s) 679
NdeII GATC 2 cut(s) 217, 1008
NgoMIV GCCGGC 1 cut(s) 173
NlaIII CATG 3 cut(s) 641, 833, 1136
NlaIV GGNNCC 4 cut(s) 248, 327, 403, 1105
NsiI ATGCAT 1 cut(s) 163
NspI RCATGY 2 cut(s) 833, 1136
OliI CACNNNNGTG 1 cut(s) 465
PceI AGGCCT 1 cut(s) 255
PcsI WCGNNNNNNNCGW 2 cut(s) 303, 363
PctI GAATGC 2 cut(s) 161, 1033
PdiI GCCGGC 1 cut(s) 175
PfeI GAWTC 3 cut(s) 14, 590, 1090
PkrI GCNGC 2 cut(s) 336, 768
PluTI GGCGCC 1 cut(s) 405
Psp6I CCWGG 1 cut(s) 923
PspFI CCCAGC 1 cut(s) 1160
PspGI CCWGG 1 cut(s) 923
PspN4I GGNNCC 4 cut(s) 248, 327, 403, 1105
PspPI GGNCC 2 cut(s) 22, 246
PsuI RGATCY 1 cut(s) 1008
RsaI GTAC 1 cut(s) 1020
RsaNI GTAC 1 cut(s) 1019
RseI CAYNNNNRTG 1 cut(s) 465
SaqAI TTAA 3 cut(s) 1166, 1173, 1180
SatI GCNGC 2 cut(s) 335, 767
Sau3AI GATC 2 cut(s) 217, 1008
Sau96I GGNCC 2 cut(s) 22, 246
ScrFI CCNGG 3 cut(s) 398, 399, 925
SduI GDGCHC 1 cut(s) 1106
SfaNI GCATC 4 cut(s) 148, 353, 736, 988
SfcI CTRYAG 2 cut(s) 1024, 1095
SfoI GGCGCC 1 cut(s) 403
SinI GGWCC 2 cut(s) 22, 246
SmaI CCCGGG 1 cut(s) 399
SmiMI CAYNNNNRTG 1 cut(s) 465
SrfI GCCCGGGC 1 cut(s) 399
Sse9I AATT 6 cut(s) 102, 241, 566, 711, 880, 1170
SseBI AGGCCT 1 cut(s) 255
SsiI CCGC 7 cut(s) 45, 166, 228, 279, 335, 429, 761
SspDI GGCGCC 1 cut(s) 401
SspMI CTAG 2 cut(s) 497, 609
StuI AGGCCT 1 cut(s) 255
StyD4I CCNGG 3 cut(s) 396, 397, 923
StyI CCWWGG 2 cut(s) 250, 1079
TaaI ACNGT 5 cut(s) 791, 1028, 1099, 1127, 1178
TaiI ACGT 3 cut(s) 300, 309, 1065
TaqI TCGA 3 cut(s) 156, 357, 751
TasI AATT 6 cut(s) 102, 241, 566, 711, 880, 1170
TauI GCSGC 1 cut(s) 337
TfiI GAWTC 3 cut(s) 14, 590, 1090
Tru1I TTAA 3 cut(s) 1166, 1173, 1180
Tru9I TTAA 3 cut(s) 1166, 1173, 1180
TscAI CASTG 4 cut(s) 469, 796, 1033, 1104
TseI GCWGC 1 cut(s) 766
TspDTI ATGAA 5 cut(s) 506, 654, 825, 975, 1062
TspGWI ACGGA 4 cut(s) 35, 175, 798, 818
TspMI CCCGGG 1 cut(s) 397
TspRI CASTG 4 cut(s) 469, 796, 1033, 1104
VpaK11BI GGWCC 2 cut(s) 22, 246
XapI RAATTY 2 cut(s) 566, 880
XceI RCATGY 2 cut(s) 833, 1136
XmaI CCCGGG 1 cut(s) 397
XspI CTAG 2 cut(s) 497, 609
ZraI GACGTC 1 cut(s) 298
Zsp2I ATGCAT 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.